Cp4.1LG01g23640 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g23640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptioncytochrome P450 724B1
LocationCp4.1LG01: 19515974 .. 19519398 (+)
RNA-Seq ExpressionCp4.1LG01g23640
SyntenyCp4.1LG01g23640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATGGGACCAAACCATTGGCTTAAGCTCAGTTCATATATGCCATGGAAGCTCCTGCAAGTTTCAGCCCTCTTACTATTGCTTTGAGCTTAGCCATGGCAGCAGTCATCCTCTGTCAGTTGCTGCTGAAACTCTTTGCAGCTGCAACGCCAAGCCCTAGCTTGCCTCCTGGCTGCATGGGCTTCCCCTTCGTTGGAGAGACTCTCAGCTTCCTCAAGCCCCATCATTCTAACTCCATTGGGACCTTCCTCCAGCGTCACTGTTCCAGGTAAATTCTAGTGTGAGATCCCACATTGGTTAGGAAGAGAACGAAGTATTCTTTATAAGCGTGTGGAACCTTTCTAACAAATGCGTTTTAAAAACCTTAAAGGAAAGTCCAAGGAGGAAAATATTTGTTGAGTCCTGAAGCGGGATGGATTGTGAGATCCTATACCGGTTGGGGAGGAGAACAAAACATTCTTTATAAGGGTGTGGAAACCTCTCTAGCAAGTGCGTTTTAAAAACCTTGAGAGAAAATCCAAGGAGGAAAATATATGTTAGCGATGAACTTAAGTTTTTACACATGGTATCAGAACCAAACTCAAACACTGGACATGTGCTAGCAAGGAAGCTGAGTCCCAAAGGGAGGGGCATGAACTTGAGTTTTTACAAATGATATCAGAACCAGACATTGGATGATGTGCTAGCAAGGAGGCTGAGCTCCGAAGGGGGTGGATTGTGAGATCTCATACCGGTTGAAGAGGAGAACAAAACATTCTTTATAAGGGTGTGGAAACCTGCATTTTAAAACCTTGTGGGAAAGTCCATAGAGTCGGGGTGGATTGTGAGATCCCACATCGGTTGAGGAGGAGAACGAAACATTGTTTATAAGAGTGCGGAAACCTCTCCCAAAGAGTTGGAGTGGATTGTGAGATCCCACATTGATTGAGAAAGAGAGCGAAACATTATTTATAATGGAGTGGAAACTCTCCTTAACACGTTTTAAAATATTGTGGGAAAGCCCAAAAAGAGGACAATATTTACTAGCCGGCTTGTGTACTTACACCGTTGGCATTGTGTATAGGTTTGGGAAGGTTTTCAAGTCTCATCTATTTGGTTCGCGGGCGATAGTGTCATGTGATCGAGAGCTTAACATGTTCATTCTTCAAAACGACGACAAACTTTTCAAAGTGAGTTATCCCAAGGCCATGCATGGCATTCTCGGCAGTAATTCTTTGATCATCGCCGCCGGAGACACCCACCGGAAGCTCAGAAACGTCGTCGTTAGCTTTGTCAGCTGGTGCAAGTCCAACCCCACTTTCCTTCACTGCGTGGACAAGCTCTCTGTCTCTTTAATGGACTCGTGGAGATCCCAAACTCGTGTGCTTTTCTGCAAAGAAGTGAAAATGGTAAAAATAAATAAAAAATACAAATAAATTATGACATAAAAATTGAATCTTTCTACAAAATTTTGAATTATTATTTTTTTATTACAGTTTGCTTTGAGTGTTATGGTGAAGGAATTGTTGGGCATTGACGCGAACGAGTCGATCGGGTCGAGAATATTGGACGAGTTCGAAACTTATATGAAAGGCTTTGTTTCTTTACCTCTGAATCTCCCTGGAACTCCATACTATAAAGCTGTTAAGGTTGTTCTCAATCTCTTTCTTCTATATCCAATATATTTCTTCAAATTTTGATTCGTACATCTAACCGTATAAAACTGCTACATGATTCAGGCCCGTGCGAAGCTTTCGGGCATCGTGAAAGAGATTATGAGAGAGAGGAAAAAGAAGGGATTAGTGGTAGGGGTGGAAAAGGAAGGGGATGATCATGAGAACTTTCTAGACGTGATAATGTCAAATTGGAAGCTTGATGAGGAAGAGATTGTGAGCGTTGTGTTGGACATATTGCTCGGAGGATACGAGACAACAGCTACTCTTATGGGATTGATTGTCTACTTTCTTGCACATTCACCTCCCAATGTTCTTGCAAACTTAAAGGTATCTAAAATCATGAATACGATCGTTCAATTCTAATGTTGTTACAAACAGTCCAAACTCAGCGTTAGTAGATATCTCCGCAATACCTCTCAAGACGTTTAAAACGCGTATATTAGGGAGAGGTTTCCACAACCTTAACGATATAGGATCTCACAATTTCCCTCTTTTCGAGGCTCAGTGTCGACACTTGTTCCCTTCTCCAATCGATGTGGGACCCTCCAATCTACCTCCCTTCGAGGGCCCAGCATCCTTGTTGGCACACCGCCTCATGTTCATCTCCTTTCAAGTCTCAGTCTCCTCACTGACAAATCGCGCCTGGTGGTGTCTGGCTCTGATACCATTTGTAATAGTCAAAGCTCACTACTCGCACAAATTGTCCTTCTTGGGCTTTCCTTTTTGAGCTTCCACTCAAAGTTTTTAAAACGCATACGTTAGGGAGTGATTTCCACACCCTTATAAAAAAAATGACTCGTTCTCCTCCCCAATCGTTGTGGGACCTCACACGTATATTTTAAATTCTCATGCATCTAACGAAGTCGTTAAAACGATGATAATAAGATGATGATGCAGGAAGAACATGAAGCAATAAGAAATGGCAAAGGGAAGGGAGAGTATCTGAATTTAGAAGATTACAAGCAAATGGAGTTCACATGCAATGTAAAAACACAACTATGAGAGCTTTTTAAAGTGCTTTTTTCTTTCTTTTTCTAAACCAATATTAGATTCATAACTCTTAAACAGGTGATATATGAAGCCATGAGATGTGGAAATGTGGTCAAATTCTTACATAGAGAAGCCATTAAAGATGTCAAATTCAAAGGTAAAATTCCTAATCCTAATTTTTAATATAATTACTTGCAGCAGCAGCAGCACCAGCAGCAGCTATTGAATTTGATTTATGGGACAGACATTTTCATTCCTTCGGGATGGAAAGTTCATCCAGTCTTCTCAGCCATTCATCTGGATCCAACCCTTCACCCATATCCTCAACAGTTCAATCCATGGAGATGGAGGGATGATAAAGAAATGAGCAAGAAGGTGACGCCCTTTGGTGGAGGCCCAAGGCTCTGTCCTGGGATTGAGCTTGCCAAGCTAGAGATAGCTTTCTTCCTCCATCATCTTGTCCTGAATTATAGGTCAGTGTTTAATTTATACACTTCCATTCAAAACAAGCGCAGATGAATATGGCTTTTTTTTTCTTTTTGGTGAGTTCTTAGGTGGAAGACAAGCGCAGATGAATGTCCACTGGCTTACCCATATGTTGAATTTAAAAGGGACTTATTGCTTGAGATTGAGCCCTGTAGTTGCTGAAATCAAAGGCTGATTAATTAAGGGAATTCTATTGTTCTAAGCTTTTGTATGCTATGATATATTATATATATATATATATCAAATTAGTTAATACTTGTTTGTTATTTCTACGCAACGTCGGGCGGAGAGAAGA

mRNA sequence

AAATGGGACCAAACCATTGGCTTAAGCTCAGTTCATATATGCCATGGAAGCTCCTGCAAGTTTCAGCCCTCTTACTATTGCTTTGAGCTTAGCCATGGCAGCAGTCATCCTCTGTCAGTTGCTGCTGAAACTCTTTGCAGCTGCAACGCCAAGCCCTAGCTTGCCTCCTGGCTGCATGGGCTTCCCCTTCGTTGGAGAGACTCTCAGCTTCCTCAAGCCCCATCATTCTAACTCCATTGGGACCTTCCTCCAGCGTCACTGTTCCAGGTTTGGGAAGGTTTTCAAGTCTCATCTATTTGGTTCGCGGGCGATAGTGTCATGTGATCGAGAGCTTAACATGTTCATTCTTCAAAACGACGACAAACTTTTCAAAGTGAGTTATCCCAAGGCCATGCATGGCATTCTCGGCAGTAATTCTTTGATCATCGCCGCCGGAGACACCCACCGGAAGCTCAGAAACGTCGTCGTTAGCTTTGTCAGCTGGTGCAAGTCCAACCCCACTTTCCTTCACTGCGTGGACAAGCTCTCTGTCTCTTTAATGGACTCGTGGAGATCCCAAACTCGTGTGCTTTTCTGCAAAGAAGTGAAAATGTTTGCTTTGAGTGTTATGGTGAAGGAATTGTTGGGCATTGACGCGAACGAGTCGATCGGGTCGAGAATATTGGACGAGTTCGAAACTTATATGAAAGGCTTTGTTTCTTTACCTCTGAATCTCCCTGGAACTCCATACTATAAAGCTGTTAAGGCCCGTGCGAAGCTTTCGGGCATCGTGAAAGAGATTATGAGAGAGAGGAAAAAGAAGGGATTAGTGGTAGGGGTGGAAAAGGAAGGGGATGATCATGAGAACTTTCTAGACGTGATAATGTCAAATTGGAAGCTTGATGAGGAAGAGATTGAAGAACATGAAGCAATAAGAAATGGCAAAGGGAAGGGAGAGTATCTGAATTTAGAAGATTACAAGCAAATGGAGTTCACATGCAATTTCAATCCATGGAGATGGAGGGATGATAAAGAAATGAGCAAGAAGGTGACGCCCTTTGGTGGAGGCCCAAGGCTCTGTCCTGGGATTGAGCTTGCCAAGCTAGAGATAGCTTTCTTCCTCCATCATCTTGTCCTGAATTATAGGTGGAAGACAAGCGCAGATGAATGTCCACTGGCTTACCCATATGTTGAATTTAAAAGGGACTTATTGCTTGAGATTGAGCCCTGTAGTTGCTGAAATCAAAGGCTGATTAATTAAGGGAATTCTATTGTTCTAAGCTTTTGTATGCTATGATATATTATATATATATATATATCAAATTAGTTAATACTTGTTTGTTATTTCTACGCAACGTCGGGCGGAGAGAAGA

Coding sequence (CDS)

ATGGAAGCTCCTGCAAGTTTCAGCCCTCTTACTATTGCTTTGAGCTTAGCCATGGCAGCAGTCATCCTCTGTCAGTTGCTGCTGAAACTCTTTGCAGCTGCAACGCCAAGCCCTAGCTTGCCTCCTGGCTGCATGGGCTTCCCCTTCGTTGGAGAGACTCTCAGCTTCCTCAAGCCCCATCATTCTAACTCCATTGGGACCTTCCTCCAGCGTCACTGTTCCAGGTTTGGGAAGGTTTTCAAGTCTCATCTATTTGGTTCGCGGGCGATAGTGTCATGTGATCGAGAGCTTAACATGTTCATTCTTCAAAACGACGACAAACTTTTCAAAGTGAGTTATCCCAAGGCCATGCATGGCATTCTCGGCAGTAATTCTTTGATCATCGCCGCCGGAGACACCCACCGGAAGCTCAGAAACGTCGTCGTTAGCTTTGTCAGCTGGTGCAAGTCCAACCCCACTTTCCTTCACTGCGTGGACAAGCTCTCTGTCTCTTTAATGGACTCGTGGAGATCCCAAACTCGTGTGCTTTTCTGCAAAGAAGTGAAAATGTTTGCTTTGAGTGTTATGGTGAAGGAATTGTTGGGCATTGACGCGAACGAGTCGATCGGGTCGAGAATATTGGACGAGTTCGAAACTTATATGAAAGGCTTTGTTTCTTTACCTCTGAATCTCCCTGGAACTCCATACTATAAAGCTGTTAAGGCCCGTGCGAAGCTTTCGGGCATCGTGAAAGAGATTATGAGAGAGAGGAAAAAGAAGGGATTAGTGGTAGGGGTGGAAAAGGAAGGGGATGATCATGAGAACTTTCTAGACGTGATAATGTCAAATTGGAAGCTTGATGAGGAAGAGATTGAAGAACATGAAGCAATAAGAAATGGCAAAGGGAAGGGAGAGTATCTGAATTTAGAAGATTACAAGCAAATGGAGTTCACATGCAATTTCAATCCATGGAGATGGAGGGATGATAAAGAAATGAGCAAGAAGGTGACGCCCTTTGGTGGAGGCCCAAGGCTCTGTCCTGGGATTGAGCTTGCCAAGCTAGAGATAGCTTTCTTCCTCCATCATCTTGTCCTGAATTATAGGTGGAAGACAAGCGCAGATGAATGTCCACTGGCTTACCCATATGTTGAATTTAAAAGGGACTTATTGCTTGAGATTGAGCCCTGTAGTTGCTGA

Protein sequence

MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIEEHEAIRNGKGKGEYLNLEDYKQMEFTCNFNPWRWRDDKEMSKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADECPLAYPYVEFKRDLLLEIEPCSC
Homology
BLAST of Cp4.1LG01g23640 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.0e-93
Identity = 191/480 (39.79%), Postives = 273/480 (56.88%), Query Frame = 0

Query: 12  IALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQR 71
           +  +L +   +L  L+L  F     +P  P G  G+P +GETL FL PH SN++G+FL+ 
Sbjct: 7   VLAALVILLALLLTLVLSHFLPLLLNPKAPKGSFGWPLLGETLRFLSPHASNTLGSFLED 66

Query: 72  HCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIAAG 131
           HCSR+G+VFKSHLF +  IVSCD+ELN FILQN+++LF+ SYP+ +HGILG +S+++  G
Sbjct: 67  HCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNEERLFQCSYPRPIHGILGKSSMLVVLG 126

Query: 132 DTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTR-------VLFCKEVKMF 191
           + H++LRN+ ++ V+  K  P++L  ++K+++ ++ SW  +++       + FC+E + F
Sbjct: 127 EDHKRLRNLALALVTSTKLKPSYLGDIEKIALHIVGSWHGKSKDKGMVNVIAFCEEARKF 186

Query: 192 ALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVK 251
           A SV+VK++LG+   E + + IL++F  +MKG +S PL +PGTPY KAV+ARA++S  VK
Sbjct: 187 AFSVIVKQVLGLSPEEPVTAMILEDFLAFMKGLISFPLYIPGTPYAKAVQARARISSTVK 246

Query: 252 EIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEE--------------------- 311
            I+ ER+      G   +GD    FLDV++S+ +L +EE                     
Sbjct: 247 GIIEERRN----AGSSNKGD----FLDVLLSSNELSDEEKVSFVLDSLLGGYETTSLLIS 306

Query: 312 ----------------IEEHEAIRNGKGKGEYLNLEDYKQMEFT---------C------ 371
                             EHE IR+ K K E+L+ EDYK+ME+T         C      
Sbjct: 307 MVVYFLGQSAQDLELVKREHEGIRSKKEKDEFLSSEDYKKMEYTQHVINEALRCGNIVKF 366

Query: 372 ----------------------------------------NFNPWRWRDDKE-MSKKVTP 391
                                                    F P RW    +  SKK TP
Sbjct: 367 VHRKALKDVRYKEYLIPSGWKVLPVFSAVHLNPLLHGNAQQFQPCRWEGASQGTSKKFTP 426

BLAST of Cp4.1LG01g23640 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.8e-65
Identity = 147/444 (33.11%), Postives = 225/444 (50.68%), Query Frame = 0

Query: 39  SLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELN 98
           +LPPG  G+P VGET  +L+ H + S+G F+++H +R+GK+++S LFG R +VS D  LN
Sbjct: 53  NLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLN 112

Query: 99  MFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCV 158
            +ILQN+ +LF+ SYP+++ GILG  S+++  GD HR++R + ++F+S  +     L  V
Sbjct: 113 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEV 172

Query: 159 DKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFV 218
           ++ ++ ++ +W   +      + K F  ++M K ++ +D  E    R+  E+ T+MKG V
Sbjct: 173 ERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVV 232

Query: 219 SLPLNLPGTPYYKAVKARAKLSGIVKEIMRERKKKGLVVGVEKEGDD------------H 278
           S PLNLPGTPY+KA+K+RA + G+++  M ER +K        E DD             
Sbjct: 233 SAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSK 292

Query: 279 ENFLDVIMSNW----------------------KLDEEEIEEHEAI-RNGKGKGE-YLNL 338
           E  LD+++S                        K  +E  EEH  I R  + +GE  L+ 
Sbjct: 293 EQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIARRQRLRGECKLSW 352

Query: 339 EDYKQMEFT-C------------------------------------------------- 387
           EDYK+M FT C                                                 
Sbjct: 353 EDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSSL 412

BLAST of Cp4.1LG01g23640 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 9.3e-63
Identity = 161/501 (32.14%), Postives = 245/501 (48.90%), Query Frame = 0

Query: 10  LTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFL 69
           L + L  ++ +++L  +LLK     T   +LPPG  G+PF+GET+ +LKP+ + ++G F+
Sbjct: 10  LPLLLLPSLLSLLLFLILLKRRNRKTRF-NLPPGKSGWPFLGETIGYLKPYTATTLGDFM 69

Query: 70  QRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIA 129
           Q+H S++GK+++S+LFG   IVS D  LN FILQN+ +LF+ SYP+++ GILG  S+++ 
Sbjct: 70  QQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVL 129

Query: 130 AGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMFALSVM 189
            GD HR +R++ ++F+S  +     L  V++ ++ ++DSW+  +      E K F  ++M
Sbjct: 130 VGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLM 189

Query: 190 VKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVKEIMRE 249
            K ++ +D  E    ++  E+ T+MKG VS PLNLPGT Y+KA+++RA +   ++  M E
Sbjct: 190 AKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEE 249

Query: 250 RK-------KKGLVVGVEKEGD----DH----------------------ENFLDVIMSN 309
           RK       ++   V  E E +    DH                      E  LD+I+S 
Sbjct: 250 RKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSL 309

Query: 310 W----------------------KLDEEEIEEH-EAIRNGKGKGE-YLNLEDYKQMEFT- 369
                                  K  EE  EEH E  R  K  GE  LN +DYK+M+FT 
Sbjct: 310 LFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQ 369

Query: 370 C------------------------------------------------------NFNPW 387
           C                                                       FNPW
Sbjct: 370 CVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPW 429

BLAST of Cp4.1LG01g23640 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 9.4e-47
Identity = 121/422 (28.67%), Postives = 190/422 (45.02%), Query Frame = 0

Query: 40  LPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSRF-GKVFKSHLFGSRAIVSCDRELN 99
           LPPG  G+P +GET S+ +   SN    F+     R+   VF SHLFGS+A++S D + N
Sbjct: 45  LPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQFN 104

Query: 100 MFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCV 159
            ++LQN+ + F+  YPKA+  ++G   L+   GD  RKL  + V+ + + +    F+  +
Sbjct: 105 KYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEI 164

Query: 160 DKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFV 219
             L  S +D W  +  +    E     L++M K+LL +  ++   + I + F  Y    +
Sbjct: 165 QNLVHSTLDRWVDKKEIALQNECHQMVLNLMAKQLLDLSPSKET-NEICELFVDYTNAVI 224

Query: 220 SLPLNLPGTPYYKAVKARAKLSGIVKEIMRERK-------KKGLVVGVEKEGDDHENFLD 279
           ++P+ +PG+ Y K +KAR  L   +  +++ER+       K  L   +E++    E   D
Sbjct: 225 AIPIKIPGSTYAKGLKARELLIRKISNMIKERRDHPHIVHKDLLTKLLEEDSISDEIICD 284

Query: 280 VIM----------------------SNWKLDEEEIEEHEAIRNGKGKGEYLNLEDYKQME 339
            I+                      +  K   +  EEH+AI   KG  + L  +DYK M+
Sbjct: 285 FILFLLFAGHETSSRAMTFAIKFLTTCPKALTQMKEEHDAILKAKGGHKKLEWDDYKSMK 344

Query: 340 FT-------------------------------------------------------CNF 375
           FT                                                         F
Sbjct: 345 FTQCVINETLRLGNFGPGVFRETKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNEALTF 404

BLAST of Cp4.1LG01g23640 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.3e-44
Identity = 129/450 (28.67%), Postives = 197/450 (43.78%), Query Frame = 0

Query: 16  LAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSR 75
           L + + I    LL L         LPPG +G P +GET   +  + + +   F+    +R
Sbjct: 7   LLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVAR 66

Query: 76  FGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHR 135
           +G VF +HLFG   I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  H+
Sbjct: 67  YGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHK 126

Query: 136 KLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLG 195
           ++ ++ +SF +        +  +D+L    +DSW S  RVL  +E K     + VK+L+ 
Sbjct: 127 RMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQLMS 186

Query: 196 IDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVKEIMRER----- 255
            D  E   S +  E+   ++GF SLPL L  T Y KA++AR K++  +  ++ +R     
Sbjct: 187 FDPGEWSES-LRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEE 246

Query: 256 ----KKKGLVVGV--EKEGDDHENFLDVIMSNWKLDEEEI-------------------- 315
               +KK ++  +    +G   E  +D +++      E                      
Sbjct: 247 EGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQ 306

Query: 316 --EEHEAIRNGKGKGEYLNLEDYKQMEFT-C----------------------------- 375
             EEHE IR  K     L   DYK M FT C                             
Sbjct: 307 LKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYK 366

BLAST of Cp4.1LG01g23640 vs. NCBI nr
Match: XP_023550585.1 (cytochrome P450 724B1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 754 bits (1947), Expect = 5.76e-273
Identity = 391/472 (82.84%), Postives = 391/472 (82.84%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI                
Sbjct: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHEAIRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPNVLANLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNQQHQQQLLN 360

Query: 361 ----------------------------------FNPWRWRDDKEMSKKVTPFGGGPRLC 391
                                             FNPWRWRDDKEMSKKVTPFGGGPRLC
Sbjct: 361 LIYGTDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSKKVTPFGGGPRLC 420

BLAST of Cp4.1LG01g23640 vs. NCBI nr
Match: XP_023550580.1 (cytochrome P450 724B1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749 bits (1935), Expect = 6.05e-271
Identity = 391/484 (80.79%), Postives = 391/484 (80.79%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI                
Sbjct: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHEAIRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPNVLANLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCG 360

Query: 361 ----------------------------------------------FNPWRWRDDKEMSK 391
                                                         FNPWRWRDDKEMSK
Sbjct: 361 NVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSK 420

BLAST of Cp4.1LG01g23640 vs. NCBI nr
Match: XP_022922169.1 (cytochrome P450 724B1 [Cucurbita moschata])

HSP 1 Score: 735 bits (1898), Expect = 2.61e-265
Identity = 384/484 (79.34%), Postives = 387/484 (79.96%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQRHCSRFGKVFKSHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGI+ANESIGSRILDEFETYMKGFVSLPLNL GTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKE+MRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEE                 
Sbjct: 241 GIVKEMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHE IRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCG 360

Query: 361 ----------------------------------------------FNPWRWRDDKEMSK 391
                                                         FNPWRWRDDKEMSK
Sbjct: 361 NVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSK 420

BLAST of Cp4.1LG01g23640 vs. NCBI nr
Match: XP_022990088.1 (cytochrome P450 724B1 [Cucurbita maxima])

HSP 1 Score: 723 bits (1865), Expect = 2.77e-260
Identity = 376/484 (77.69%), Postives = 384/484 (79.34%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPL+IALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLSIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQ HCSRFG VFKSHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLH VDKLSVSLM+SWR QTRV FCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGI+ANES+G RILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKE+M+ERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI                
Sbjct: 241 GIVKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHEAIRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCG 360

Query: 361 ----------------------------------------------FNPWRWRDDKEMSK 391
                                                         FNPWRWRDDKEMSK
Sbjct: 361 NVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSK 420

BLAST of Cp4.1LG01g23640 vs. NCBI nr
Match: XP_023550591.1 (cytochrome P450 724B1 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706 bits (1823), Expect = 1.51e-254
Identity = 368/443 (83.07%), Postives = 372/443 (83.97%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIEE---------HEAIR 300
           GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI           +E   
Sbjct: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTA 300

Query: 301 NGKGKGEYL----------NLEDYKQME-------------------------------- 360
              G   Y           NL+   Q +                                
Sbjct: 301 TLMGLIVYFLAHSPPNVLANLKQQHQQQLLNLIYGTDIFIPSGWKVHPVFSAIHLDPTLH 360

Query: 361 -FTCNFNPWRWRDDKEMSKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADE 391
            +   FNPWRWRDDKEMSKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADE
Sbjct: 361 PYPQQFNPWRWRDDKEMSKKVTPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADE 420

BLAST of Cp4.1LG01g23640 vs. ExPASy TrEMBL
Match: A0A6J1E2M5 (cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 1.26e-265
Identity = 384/484 (79.34%), Postives = 387/484 (79.96%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQRHCSRFGKVFKSHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGI+ANESIGSRILDEFETYMKGFVSLPLNL GTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKE+MRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEE                 
Sbjct: 241 GIVKEMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHE IRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCG 360

Query: 361 ----------------------------------------------FNPWRWRDDKEMSK 391
                                                         FNPWRWRDDKEMSK
Sbjct: 361 NVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSK 420

BLAST of Cp4.1LG01g23640 vs. ExPASy TrEMBL
Match: A0A6J1JS89 (cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1)

HSP 1 Score: 723 bits (1865), Expect = 1.34e-260
Identity = 376/484 (77.69%), Postives = 384/484 (79.34%), Query Frame = 0

Query: 1   MEAPASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60
           MEAPASFSPL+IALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH
Sbjct: 1   MEAPASFSPLSIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPH 60

Query: 61  HSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120
           HSNSIGTFLQ HCSRFG VFKSHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGI
Sbjct: 61  HSNSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGI 120

Query: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKE 180
           LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLH VDKLSVSLM+SWR QTRV FCKE
Sbjct: 121 LGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKE 180

Query: 181 VKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240
           VKMFALSVMVKELLGI+ANES+G RILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS
Sbjct: 181 VKMFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLS 240

Query: 241 GIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI---------------- 300
           GIVKE+M+ERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI                
Sbjct: 241 GIVKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTA 300

Query: 301 ----------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN--------- 360
                                 EEHEAIRNGKGKGEYLNLEDYKQMEFTCN         
Sbjct: 301 TLMGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCG 360

Query: 361 ----------------------------------------------FNPWRWRDDKEMSK 391
                                                         FNPWRWRDDKEMSK
Sbjct: 361 NVVKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSK 420

BLAST of Cp4.1LG01g23640 vs. ExPASy TrEMBL
Match: A0A6J1BWX9 (cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1)

HSP 1 Score: 570 bits (1468), Expect = 3.50e-200
Identity = 307/486 (63.17%), Postives = 339/486 (69.75%), Query Frame = 0

Query: 3   APASFSPLTI---ALSLAMAAVILCQLL-LKLFAAATPSPSLPPGCMGFPFVGETLSFLK 62
           A AS SP TI   ALSLAMAA+I   LL L +  AA   P+LPPG MGFPF+G+TL  LK
Sbjct: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65

Query: 63  PHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMH 122
           PH SNSIGTFL  HCSR+GKVFKSHLFG  AIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125

Query: 123 GILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFC 182
           GILG+NSLIIAAGDTHRKLRNVVVSF+ WCKS P+FLHCV  L++SLMDSWRSQ +V FC
Sbjct: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185

Query: 183 KEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAK 242
           KEVKMFALS+MVKELLGI+A E+IGS+IL+EFE YMKGFVSLP+NLPGT Y  AVKAR +
Sbjct: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245

Query: 243 LSGIVKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEI-------------- 302
           LS +VKE+MRERK++G+VVG +      E+FL VIMSNWKL++EEI              
Sbjct: 246 LSCMVKEMMRERKRRGMVVGAD------EDFLQVIMSNWKLNDEEIVSVVLDILLGGYET 305

Query: 303 ------------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN------- 362
                                   EEHEAIR  K  GE LNLEDYKQMEFT N       
Sbjct: 306 TATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMR 365

Query: 363 ------------------------------------------------FNPWRWRDDKEM 391
                                                           FNPWRW DDKEM
Sbjct: 366 CGNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDKEM 425

BLAST of Cp4.1LG01g23640 vs. ExPASy TrEMBL
Match: A0A1S3C8C0 (cytochrome P450 724B1 OS=Cucumis melo OX=3656 GN=LOC103498124 PE=3 SV=1)

HSP 1 Score: 530 bits (1365), Expect = 1.66e-184
Identity = 288/480 (60.00%), Postives = 330/480 (68.75%), Query Frame = 0

Query: 6   SFSPLTIALSLAM--AAVILCQLLLK-LFAAATPSPSLPPGCMGFPFVGETLSFLKPHHS 65
           + SP+ I L LA+  A  I+  LLLK LF  +  +P+LPPG MG PFVGETLSFL PHHS
Sbjct: 7   NLSPMIIILVLALSFAISIIFHLLLKFLFVISKQNPNLPPGSMGLPFVGETLSFLHPHHS 66

Query: 66  NSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 125
           NSIG+FLQ H SR+GK+FKSHLFG  AIVSCDRELN F+LQNDDK FKVSYPKAMH ILG
Sbjct: 67  NSIGSFLQHHFSRYGKIFKSHLFGRPAIVSCDRELNNFVLQNDDKFFKVSYPKAMHDILG 126

Query: 126 SNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVK 185
            NSLIIAAGDTHRKLR+V+VSF+S CK+ P FLH +  LSVSL+DSWRS+T+V F KE+K
Sbjct: 127 KNSLIIAAGDTHRKLRSVIVSFISGCKTRPIFLHSLHNLSVSLIDSWRSRTQVSFVKEMK 186

Query: 186 MFALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGI 245
           MFALS+M+KE+ GIDA ESIGS+I +EFETYM GFVSLPLNLPGTPY+KAVKAR +LS I
Sbjct: 187 MFALSLMLKEVFGIDAKESIGSKIFEEFETYMIGFVSLPLNLPGTPYFKAVKARGRLSTI 246

Query: 246 VKEIMRERKKKGLVVGVEKEGDDHENFLDVIMSN-WKLDEEEI----------------- 305
           VKE+MRER+K+ L+ G     +++E+FL V+MSN WKL +EEI                 
Sbjct: 247 VKEMMRERRKRELLGG----NNNNEDFLQVLMSNNWKLSDEEIVSVALDILLGSYETTAT 306

Query: 306 ---------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN---------- 365
                                EEH+AIRNGKG GE L+LEDYKQMEFT N          
Sbjct: 307 LLGLIVYFLVHSPPKILAKLKEEHQAIRNGKGNGECLDLEDYKQMEFTYNVTYEAMRCGN 366

Query: 366 ---------------------------------------------FNPWRWRDDKEMSKK 388
                                                        FNPWRW DDKEM+KK
Sbjct: 367 VVKFLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPWRWNDDKEMNKK 426

BLAST of Cp4.1LG01g23640 vs. ExPASy TrEMBL
Match: A0A0A0KNY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 6.66e-179
Identity = 281/479 (58.66%), Postives = 324/479 (67.64%), Query Frame = 0

Query: 6   SFSPLTI-ALSLAMAAVILCQLLLKLFAAATP-SPSLPPGCMGFPFVGETLSFLKPHHSN 65
           + SP+ I  L+L+ A  I+  LLLKLF   +  +P+LPPG MG PFVGETLSFL PHHSN
Sbjct: 7   NLSPMIIIVLALSFAISIIFHLLLKLFLVTSKKNPNLPPGSMGLPFVGETLSFLNPHHSN 66

Query: 66  SIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGS 125
           SIGTFLQ H SR+GK+FKS LFG  AIVSCDRELN F+LQNDDKLFKVSYPKAMH ILG+
Sbjct: 67  SIGTFLQHHFSRYGKIFKSRLFGRPAIVSCDRELNYFVLQNDDKLFKVSYPKAMHNILGT 126

Query: 126 NSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKM 185
           NSL+I+AGDTHRKLR+V+VSF++ CK+ P FLH +  LSVSL DSWRSQT V F KE+KM
Sbjct: 127 NSLLISAGDTHRKLRSVIVSFITRCKTRPNFLHSLHNLSVSLTDSWRSQTHVSFIKEMKM 186

Query: 186 FALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIV 245
           FALS+MVKE+ GI+A E IG++I +EFET+M GFVSLPLN PGTPY+KAVKAR +LS IV
Sbjct: 187 FALSLMVKEVFGIEAKELIGTKIFEEFETFMIGFVSLPLNFPGTPYFKAVKARGRLSTIV 246

Query: 246 KEIMRERKKKGLVVGVEKEGDDHENFLDVIMSN-WKLDEEEI------------------ 305
           KE++RER+K+GLV G   + D    FL V+MSN WKL +EEI                  
Sbjct: 247 KEMIRERRKRGLVGGNNNDDD----FLQVLMSNNWKLSDEEIVSVVLDIMLGSYETTATL 306

Query: 306 --------------------EEHEAIRNGKGKGEYLNLEDYKQMEFTCN----------- 365
                               EEH+AIRNGK KGE LNLEDYK+MEFT N           
Sbjct: 307 LGLIVYFLAHSPPNILAKLKEEHQAIRNGKRKGECLNLEDYKRMEFTFNVAYEAMRCGNV 366

Query: 366 --------------------------------------------FNPWRWRDDKEMSKKV 388
                                                       FNP RW DDKEM+KKV
Sbjct: 367 VKFLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPSRWSDDKEMNKKV 426

BLAST of Cp4.1LG01g23640 vs. TAIR 10
Match: AT5G14400.1 (cytochrome P450, family 724, subfamily A, polypeptide 1 )

HSP 1 Score: 292.0 bits (746), Expect = 7.3e-79
Identity = 166/370 (44.86%), Postives = 210/370 (56.76%), Query Frame = 0

Query: 99  MFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCV 158
           MFILQN+ KLF   YPKAMH ILG  SL++A G+ HRKL+NV++SF++  KS P FLHC 
Sbjct: 1   MFILQNEGKLFTSDYPKAMHDILGKYSLLLATGEIHRKLKNVIISFINLTKSKPDFLHCA 60

Query: 159 DKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFV 218
           + LS+S++ SW++   V F KEVK+F LSVMV +LL I   +     +L +F +YMKGF+
Sbjct: 61  ENLSISILKSWKNCREVEFHKEVKIFTLSVMVNQLLSIKPEDPARLYVLQDFLSYMKGFI 120

Query: 219 SLPLNLPGTPYYKAVKARAKLSGIVKEIMRERKKKGLVVGVEKEGDD------HENFLDV 278
           SLP+ LPGT Y  A+KAR +LS  V  +++ER         E+E +D       E+FLD 
Sbjct: 121 SLPIPLPGTGYTNAIKARKRLSARVMGMIKER---------EREEEDMNNAIREEDFLDS 180

Query: 279 IMSNWKLDEEE-------------------------------------IEEHEAIRNGKG 338
           I+SN  L+ EE                                      EEH AIR  KG
Sbjct: 181 IISNEDLNYEEKVSIVLDILLGGFETSATTLSLVVYFLAKSPNLLHKLKEEHAAIRAKKG 240

Query: 339 KGEYLNLEDYKQMEFT-------------------------------------CNFNPWR 389
            GE LN EDY++MEFT                                       FNP R
Sbjct: 241 DGELLNWEDYQKMEFTQCVISEALRCEYVIPKGWKVFPIFTAVHLDPSLHENPFEFNPMR 300

BLAST of Cp4.1LG01g23640 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 6.6e-64
Identity = 161/501 (32.14%), Postives = 245/501 (48.90%), Query Frame = 0

Query: 10  LTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFL 69
           L + L  ++ +++L  +LLK     T   +LPPG  G+PF+GET+ +LKP+ + ++G F+
Sbjct: 10  LPLLLLPSLLSLLLFLILLKRRNRKTRF-NLPPGKSGWPFLGETIGYLKPYTATTLGDFM 69

Query: 70  QRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIA 129
           Q+H S++GK+++S+LFG   IVS D  LN FILQN+ +LF+ SYP+++ GILG  S+++ 
Sbjct: 70  QQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVL 129

Query: 130 AGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMFALSVM 189
            GD HR +R++ ++F+S  +     L  V++ ++ ++DSW+  +      E K F  ++M
Sbjct: 130 VGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLM 189

Query: 190 VKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVKEIMRE 249
            K ++ +D  E    ++  E+ T+MKG VS PLNLPGT Y+KA+++RA +   ++  M E
Sbjct: 190 AKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEE 249

Query: 250 RK-------KKGLVVGVEKEGD----DH----------------------ENFLDVIMSN 309
           RK       ++   V  E E +    DH                      E  LD+I+S 
Sbjct: 250 RKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSL 309

Query: 310 W----------------------KLDEEEIEEH-EAIRNGKGKGE-YLNLEDYKQMEFT- 369
                                  K  EE  EEH E  R  K  GE  LN +DYK+M+FT 
Sbjct: 310 LFAGHETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQ 369

Query: 370 C------------------------------------------------------NFNPW 387
           C                                                       FNPW
Sbjct: 370 CVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPW 429

BLAST of Cp4.1LG01g23640 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-45
Identity = 129/450 (28.67%), Postives = 197/450 (43.78%), Query Frame = 0

Query: 16  LAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSR 75
           L + + I    LL L         LPPG +G P +GET   +  + + +   F+    +R
Sbjct: 7   LLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVAR 66

Query: 76  FGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHR 135
           +G VF +HLFG   I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  H+
Sbjct: 67  YGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHK 126

Query: 136 KLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLG 195
           ++ ++ +SF +        +  +D+L    +DSW S  RVL  +E K     + VK+L+ 
Sbjct: 127 RMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQLMS 186

Query: 196 IDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVKEIMRER----- 255
            D  E   S +  E+   ++GF SLPL L  T Y KA++AR K++  +  ++ +R     
Sbjct: 187 FDPGEWSES-LRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEE 246

Query: 256 ----KKKGLVVGV--EKEGDDHENFLDVIMSNWKLDEEEI-------------------- 315
               +KK ++  +    +G   E  +D +++      E                      
Sbjct: 247 EGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQ 306

Query: 316 --EEHEAIRNGKGKGEYLNLEDYKQMEFT-C----------------------------- 375
             EEHE IR  K     L   DYK M FT C                             
Sbjct: 307 LKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYK 366

BLAST of Cp4.1LG01g23640 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-43
Identity = 122/443 (27.54%), Postives = 198/443 (44.70%), Query Frame = 0

Query: 40  LPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNM 99
           +P G +G+P +GETL+F+   +S+   TF+ +  S +GKVFK+++ G+  I+S D E+N 
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 100 FILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVD 159
            +LQN    F  +YPK++  +LG NS++   G   ++L  ++ +F+            ++
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 160 KLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGFVS 219
              V  + SW     V    E+K     ++VK L+     E +    L EFE ++KG + 
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKL-EFEEFIKGLIC 248

Query: 220 LPLNLPGTPYYKAVKARAKLSGIVKEIMRERK--------KKGLVVGVEKEGDDHE---- 279
           +P+  PGT  YK++KA+ +L  +VK+++ ER+           +V  + ++G D E    
Sbjct: 249 IPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQ 308

Query: 280 --NFLDVIMSNWKLDEEE-----------------------IEEHEAIRNGKGK-GEYLN 339
             +F+   +    +  EE                       +EE+  ++  K + GE   
Sbjct: 309 PSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYK 368

Query: 340 LEDYKQMEFTCN------------------------------------------------ 387
             DY  + FT N                                                
Sbjct: 369 WTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDED 428

BLAST of Cp4.1LG01g23640 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 172.9 bits (437), Expect = 4.9e-43
Identity = 118/445 (26.52%), Postives = 196/445 (44.04%), Query Frame = 0

Query: 38  PSLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQRHCSRFGKVFKSHLFGSRAIVSCDREL 97
           P  P G +G+P +GET+ F+   +S+   +F+ +    +G+VFKSH+FG+  IVS D E+
Sbjct: 49  PKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEV 108

Query: 98  NMFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHC 157
           N  +LQ+D   F   YPK +  ++G +S+++  G  HR+   +V SF+         +  
Sbjct: 109 NRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRD 168

Query: 158 VDKLSVSLMDSWRSQTRVLFCKEVKMFALSVMVKELLGIDANESIGSRILDEFETYMKGF 217
           + K     MD W     VL     K  A  V+ K L+ ++  E +   +  EFE ++ G 
Sbjct: 169 MHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDL-EELKREFENFISGL 228

Query: 218 VSLPLNLPGTPYYKAVKARAKLSGIVKEI----MRERKK--------KGLVVGVEKEGDD 277
           +SLP+N PGT  +++++A+  +   V+ I    +R+ K         K +V  + K+  +
Sbjct: 229 MSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSE 288

Query: 278 H-------ENFLDVIM---------------------SNWKLDEEEIEEHEAIRNGKGKG 337
           H        N +D+++                     +   L  EE  + ++++   G+ 
Sbjct: 289 HLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEP 348

Query: 338 EYLNLEDYKQMEFT---------------------------------------------- 388
            Y N  DY  + FT                                              
Sbjct: 349 LYWN--DYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVH 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6F4F51.0e-9339.79Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 ... [more]
Q5CCK35.8e-6533.11Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV... [more]
O649899.3e-6332.14Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Q50EK69.4e-4728.67Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1[more]
Q425694.3e-4428.67Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023550585.15.76e-27382.84cytochrome P450 724B1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023550580.16.05e-27180.79cytochrome P450 724B1 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022922169.12.61e-26579.34cytochrome P450 724B1 [Cucurbita moschata][more]
XP_022990088.12.77e-26077.69cytochrome P450 724B1 [Cucurbita maxima][more]
XP_023550591.11.51e-25483.07cytochrome P450 724B1 isoform X3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1E2M51.26e-26579.34cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1[more]
A0A6J1JS891.34e-26077.69cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1[more]
A0A6J1BWX93.50e-20063.17cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1[more]
A0A1S3C8C01.66e-18460.00cytochrome P450 724B1 OS=Cucumis melo OX=3656 GN=LOC103498124 PE=3 SV=1[more]
A0A0A0KNY16.66e-17958.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14400.17.3e-7944.86cytochrome P450, family 724, subfamily A, polypeptide 1 [more]
AT3G50660.16.6e-6432.14Cytochrome P450 superfamily protein [more]
AT5G05690.13.1e-4528.67Cytochrome P450 superfamily protein [more]
AT4G36380.11.3e-4327.54Cytochrome P450 superfamily protein [more]
AT3G13730.14.9e-4326.52cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 339..362
score: 30.82
coord: 73..92
score: 22.19
coord: 329..339
score: 49.55
IPR001128Cytochrome P450PFAMPF00067p450coord: 313..368
e-value: 7.2E-12
score: 44.8
coord: 41..284
e-value: 2.4E-5
score: 23.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 311..379
e-value: 8.5E-15
score: 56.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..293
e-value: 1.8E-43
score: 150.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..369
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 44..283
coord: 285..313
coord: 313..388
NoneNo IPR availablePANTHERPTHR24286:SF159CYTOCHROME P450, FAMILY 724, SUBFAMILY A, POLYPEPTIDE 1coord: 44..283
coord: 313..388
NoneNo IPR availablePANTHERPTHR24286:SF159CYTOCHROME P450, FAMILY 724, SUBFAMILY A, POLYPEPTIDE 1coord: 285..313
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 332..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g23640.1Cp4.1LG01g23640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901615 organic hydroxy compound metabolic process
biological_process GO:0065008 regulation of biological quality
biological_process GO:0008202 steroid metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen