Cp4.1LG01g23380 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g23380
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionChaperone protein DnaJ
LocationCp4.1LG01: 19622373 .. 19625717 (-)
RNA-Seq ExpressionCp4.1LG01g23380
SyntenyCp4.1LG01g23380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTGTTTTTCTTTTCTTTTTATGGCATTAGAATATAAATTTTATTAAAATTATTTATAGTATAAATTTGATTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNAGAATAAAACAAAAATTGAGATACTCACGGAAGAGAATATTCCGCTAAAGGCGGAAATAATTTTCCAATATAAAAGCGACTTGGTGGCACTTCAGAAATATCTCGAATCCCGAGAAATATCTCATCCATTTCGTACTCTTTGATCTTCATTCCTCCACTGGACTGTCGATTGGCGGCGAAGTTGCAGTTTCATTCGTCCTCATGGCCATCTCCATTGTTCCATCTATTCAACGCTCGGTGCCAATGGCTTCTTCATCTTTTGCGCATCCTAATGAACCTAAGCATATCATAAGGTAATTTTCTTTACAGAGATCGATCATATTGTCAAGAGTTCTATCTTCTTCATCTTTTTAGTCTTTTATTTTGGAGTGGAAGAGATCTTTTACATTTGACGAGCTTTGCGGCTCCAATTTTCTTCTATATAATACTTCTGACGAACAATTTTGGCCTGAAGGAAGCCGAGATGGACGGCGATTAGATGCAGCGCAAGAGAAAGAGCGAACCAGACGAACTATTACGAGCTGCTTGGAGTTTCTGCAGATTCAAATGCTAAAGAAATCAAAGAAGCTTACAGAAAGTTACAGAAGAAATTTCATCCGGATATTGCAGGCCAAGAGGTTGTTCATTTGTACCAGAATTCATTGTAAACAAACTACCTTCTGATGATTTTTTTAAAATTGCCAGGGCCACGAGCGTACGTTGAGATTGAACGAGGCTTATCGAGTTCTGATGAGAGAGGATCAAAGGAAGGAATATGATTCTTCCATGGGTGAGACGAGGGTGAGATTTGGGAATTATAACCCTGGATTAGGTTACAGCTCCTGGAAAGGACCTCTGCGATCTCAGGCTTTGTTTGTGGACGGAAACGCCTGCATTGGTATGGTTGTGTTCATCCTTTACTATTCATTTTGTTAATGAACTCTGCCAAGTAACAATATTGCGAAAAGATCTCGAAGCCTGGAGAGACTTTTGTCGATTATGCAGTATTGCGAAAAGATCTCGAAGCCTGGAGAGACTTTTGTCGATTATGCAGTTTGATTTTGTGGCGACATATACGCATGATTATGATTGGATTTGGATGTTCTTATTGTTGTACTCGAGTTTCTTGATGTCTAAAATTTTTGTGAATGTTGACTTAGAACACGATCTTTTTAGCTTAGATTCATCACTAGTTCGACTTAAACTCGCCAAGTTACATGGATAAGAAATAGTAAAAAGGATTGGAACTCGTTATTATTTATCTGAAACCACTTTCAAATATGAAGATGAGAAGCTTGCATAATTTCTCACAGGTTGCAGGGAATGTGTACACCATGCAAGTAATACATTCACCATGGACGAGACTCTTGGTCGTGCACGAGTTAAGGTTCAGTATGGAGACAATGATCAGAAAATTCAGGTAAAACGGGGGAAAATGTAAATTACGAGTAGAAAAAGAACACTAAACAGCTTCGCTTCATTCTTACAGGTTTCAGTGGAATCATGTCCCGTAAATTGCATATATTGGGTAGAAAAGGAGGAACTGGCTGTGTTGGAGTATCTGATGCAGCCACTGCCCAAGGAAGAATATGGCGTGTTTGGAGGCGGCTGGGAAATTCCGGCCAATGTCTTCATGGCGGCCAAAGCCTTCACCCAGCAGCTGAAACGACAGAAACAGGACACAAAATTTGGTGAGTTATGAAGCTATTCTCAACTGGCTTTTAGTTCAATTCGATCACTTATGTGAAGATTTACAGGAAAGGAAACGGTTGAAGAAACCCCAGCTCAAGCAGAGGCTCGAGCTAATGCGAGCATGAAAATACAGATGGAGAAGATCAATGGAATTTGGAATTGGGTAAAACAAGTTTTTGGGACACACAGGTAGATTCATGAAAAGCTATGAATTTCAATCACTATATCCAATAGTTTGTTTGGAAAACTGTTCTCAAAACCTTTCTGAGAACTAATATTATTGTGCATATTGTAGTCATTTGTGAAAATAGAATGAAGATCAAGCTTACAAACTTAGATACCAAACAAAGATAGATTCTAAAGCCTTCAGTAGTCACTGATATACAAACCCATCCAATCCTCACCACCGAACACGTTCACTCGAAACGAAACTACGTCGTTTCATCCCAAGTCCCTACTTTCATAGACTCATTAATTAAATCTGCATTTGCCTGTTCCAGCACAAACTTCACATTCATCTGCAACTCCAGCAGCATGACTCAAGCAGTGATTCATTCTATCATTCACAACATCCTGCCAGTGCAAACAGAACACCTCACATTTCAATACTAACAAATACAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNGTTTCTTCACTATACTGGGAAAAGAAATCTACTTGAGAAATTCCTACCACAAGCTTTTCATGAAGACCAAGAGGAGCAGTTACTATGTATGATATTCCTGGGTGGTCCTTTGCTGCCTCAGCTGTTAAGGAAGGAATATCCTACAAAATTCCAGCCATTGCTATCAATCCACTTACTGAAAGAGTCATTTCATCAAATCAACTGCCTTACGCAAGTTACCTGTTGCCAATGTCGACCGGGGAAGAGGAAGAACGGGCTTATGATGATGCGATTGGCCCCTTGTTCAACGCACGATTTGAATGCATCTCTTATCGAAGGTTTAGCCAGCTCCTGTGGTTTTTAGAGCAAAGTAAAAGAGAATTCATTACAGCCCCACAAGAAAAATGCAGTTAAGAAGAACATACCATGTGAGCAGGCTCCACAATAGGGTAACCAGTGTTATCCCTAAACATAGCAACGAACTCATCTGAAATTCACAATGAAATTAGATTGCGTAGAATTTCTAATCTCACCCAAGTTCCAAAACCCTAACTTACTCAGCATGAGGTTAGATTCCTGCCGCCGGGAGCCATGGTCGACGATAATGACTGCATCTCCAGCACCAATCCGATCTCGGCTCCGACTCGAGCCTCCATTATCGACACTCATGCGGAATCGCGAGGTGGAATTTCCGAATCTCAAGTGCCGTCCGATAGGAATGGCAGATTTCTGAGATGTGCTGATTGTGTATGTTCGTCTTCTAGTGTCGCCGGACGAGAAACTTCAATGGCGAAACGAACGGCCATTAGAGGCAGATTAAGCAAATTGAAAAGGGCAAAATTAGAGGGAAGATTTTGCAGACCTCTTCACAGTGATTTGAGGAGTGATGGATAA

mRNA sequence

ATGGCATTAAATATCTCGAATCCCGAGAAATATCTCATCCATTTCGTACTCTTTGATCTTCATTCCTCCACTGGACTGTCGATTGGCGGCGAAGTTGCAGTTTCATTCGTCCTCATGGCCATCTCCATTGTTCCATCTATTCAACGCTCGGTGCCAATGGCTTCTTCATCTTTTGCGCATCCTAATGAACCTAAGCATATCATAAGGAAGCCGAGATGGACGGCGATTAGATGCAGCGCAAGAGAAAGAGCGAACCAGACGAACTATTACGAGCTGCTTGGAGTTTCTGCAGATTCAAATGCTAAAGAAATCAAAGAAGCTTACAGAAAGTTACAGAAGAAATTTCATCCGGATATTGCAGGCCAAGAGGGCCACGAGCGTACGTTGAGATTGAACGAGGCTTATCGAGTTCTGATGAGAGAGGATCAAAGGAAGGAATATGATTCTTCCATGGGTGAGACGAGGGTGAGATTTGGGAATTATAACCCTGGATTAGGTTACAGCTCCTGGAAAGGACCTCTGCGATCTCAGGCTTTGTTTGTGGACGGAAACGCCTGCATTGGTTGCAGGGAATGTGTACACCATGCAAGTAATACATTCACCATGGACGAGACTCTTGGTCGTGCACGAGTTAAGGTTCAGTATGGAGACAATGATCAGAAAATTCAGGTTTCAGTGGAATCATGTCCCGTAAATTGCATATATTGGGTAGAAAAGGAGGAACTGGCTGTGTTGGAGTATCTGATGCAGCCACTGCCCAAGGAAGAATATGGCGTGTTTGGAGGCGGCTGGGAAATTCCGGCCAATGTCTTCATGGCGGCCAAAGCCTTCACCCAGCAGCTGAAACGACAGAAACAGGACACAAAATTTGGAAAGGAAACGGTTGAAGAAACCCCAGCTCAAGCAGAGGCTCGAGCTAATGCGAGCATGAAAATACAGATGGAGAAGATCAATGGAATTTGGAATTGGGTAAAACAAGTTTTTGGGACACACAGACCAAGAGGAGCAGTTACTATGTATGATATTCCTGGGTGGTCCTTTGCTGCCTCAGCTGTTAAGGAAGGAATATCCTACAAAATTCCAGCCATTGCTATCAATCCACTTACTGAAAGAGTCATTTCATCAAATCAACTGCCTTACGCAAGTTACCTGTTGCCAATGTCGACCGGGGAAGAGGAAGAACGGGCTTATGATGATGCGATTGGCCCCTTGTTCAACGCACGATTTGAATGCATCTCTTATCGAAGATTCCTGCCGCCGGGAGCCATGGTCGACGATAATGACTGCATCTCCAGCACCAATCCGATCTCGGCTCCGACTCGAGCCTCCATTATCGACACTCATGCGGAATCGCGAGGTGGAATTTCCGAATCTCAAGTGCCGTCCGATAGGAATGGCAGATTTCTGAGATGTGCTGATTGTGTATGTTCGTCTTCTAGTGTCGCCGGACGAGAAACTTCAATGGCGAAACGAACGGCCATTAGAGGCAGATTAAGCAAATTGAAAAGGGCAAAATTAGAGGGAAGATTTTGCAGACCTCTTCACAGTGATTTGAGGAGTGATGGATAA

Coding sequence (CDS)

ATGGCATTAAATATCTCGAATCCCGAGAAATATCTCATCCATTTCGTACTCTTTGATCTTCATTCCTCCACTGGACTGTCGATTGGCGGCGAAGTTGCAGTTTCATTCGTCCTCATGGCCATCTCCATTGTTCCATCTATTCAACGCTCGGTGCCAATGGCTTCTTCATCTTTTGCGCATCCTAATGAACCTAAGCATATCATAAGGAAGCCGAGATGGACGGCGATTAGATGCAGCGCAAGAGAAAGAGCGAACCAGACGAACTATTACGAGCTGCTTGGAGTTTCTGCAGATTCAAATGCTAAAGAAATCAAAGAAGCTTACAGAAAGTTACAGAAGAAATTTCATCCGGATATTGCAGGCCAAGAGGGCCACGAGCGTACGTTGAGATTGAACGAGGCTTATCGAGTTCTGATGAGAGAGGATCAAAGGAAGGAATATGATTCTTCCATGGGTGAGACGAGGGTGAGATTTGGGAATTATAACCCTGGATTAGGTTACAGCTCCTGGAAAGGACCTCTGCGATCTCAGGCTTTGTTTGTGGACGGAAACGCCTGCATTGGTTGCAGGGAATGTGTACACCATGCAAGTAATACATTCACCATGGACGAGACTCTTGGTCGTGCACGAGTTAAGGTTCAGTATGGAGACAATGATCAGAAAATTCAGGTTTCAGTGGAATCATGTCCCGTAAATTGCATATATTGGGTAGAAAAGGAGGAACTGGCTGTGTTGGAGTATCTGATGCAGCCACTGCCCAAGGAAGAATATGGCGTGTTTGGAGGCGGCTGGGAAATTCCGGCCAATGTCTTCATGGCGGCCAAAGCCTTCACCCAGCAGCTGAAACGACAGAAACAGGACACAAAATTTGGAAAGGAAACGGTTGAAGAAACCCCAGCTCAAGCAGAGGCTCGAGCTAATGCGAGCATGAAAATACAGATGGAGAAGATCAATGGAATTTGGAATTGGGTAAAACAAGTTTTTGGGACACACAGACCAAGAGGAGCAGTTACTATGTATGATATTCCTGGGTGGTCCTTTGCTGCCTCAGCTGTTAAGGAAGGAATATCCTACAAAATTCCAGCCATTGCTATCAATCCACTTACTGAAAGAGTCATTTCATCAAATCAACTGCCTTACGCAAGTTACCTGTTGCCAATGTCGACCGGGGAAGAGGAAGAACGGGCTTATGATGATGCGATTGGCCCCTTGTTCAACGCACGATTTGAATGCATCTCTTATCGAAGATTCCTGCCGCCGGGAGCCATGGTCGACGATAATGACTGCATCTCCAGCACCAATCCGATCTCGGCTCCGACTCGAGCCTCCATTATCGACACTCATGCGGAATCGCGAGGTGGAATTTCCGAATCTCAAGTGCCGTCCGATAGGAATGGCAGATTTCTGAGATGTGCTGATTGTGTATGTTCGTCTTCTAGTGTCGCCGGACGAGAAACTTCAATGGCGAAACGAACGGCCATTAGAGGCAGATTAAGCAAATTGAAAAGGGCAAAATTAGAGGGAAGATTTTGCAGACCTCTTCACAGTGATTTGAGGAGTGATGGATAA

Protein sequence

MALNISNPEKYLIHFVLFDLHSSTGLSIGGEVAVSFVLMAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRFGNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFTQQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHRPRGAVTMYDIPGWSFAASAVKEGISYKIPAIAINPLTERVISSNQLPYASYLLPMSTGEEEERAYDDAIGPLFNARFECISYRRFLPPGAMVDDNDCISSTNPISAPTRASIIDTHAESRGGISESQVPSDRNGRFLRCADCVCSSSSVAGRETSMAKRTAIRGRLSKLKRAKLEGRFCRPLHSDLRSDG
Homology
BLAST of Cp4.1LG01g23380 vs. ExPASy Swiss-Prot
Match: Q9FMX6 (Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC76 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.1e-34
Identity = 95/238 (39.92%), Postives = 130/238 (54.62%), Query Frame = 0

Query: 90  YELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDS 149
           Y+LLG+   S+  +IK AYR LQK+ HPDIAG  GH+  + LNEAY++L     R+ YD 
Sbjct: 52  YDLLGIDRSSDKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDK 111

Query: 150 SMGETRVRFGNYNPGLGYSSWKGPLRSQ-ALFVDGNACIGCRECVHHASNTFTMDETLGR 209
              +     G Y     YS W GP   Q A FVD   C+GC +C   A  TF ++   GR
Sbjct: 112 EQAKLEELRG-YTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGR 171

Query: 210 ARVKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLM--QPLPKEEYGVFGGGWEI 269
           ARV  Q+ D + KI+ ++E+CPV+CI  VE+ +LA LE+LM  QP      GV     E 
Sbjct: 172 ARVVAQWADPESKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGER 231

Query: 270 PANVFMAAKAFTQQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWV 325
            +NVF+  K F      Q++  K    T +ET +Q E + +A     +E I  I NW+
Sbjct: 232 VSNVFVDVKKF------QERYAKAMSRTTKET-SQREVQISA-----VEAIRSISNWL 276

BLAST of Cp4.1LG01g23380 vs. ExPASy Swiss-Prot
Match: Q75VW3 (Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) OX=608538 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-11
Identity = 43/94 (45.74%), Postives = 59/94 (62.77%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQE-GHERTLRLNEAYRVLMREDQRKE 147
           +YY++LGVS D+ A+EIK+AYR+L K++HPDI+  E   E+   +NEAY +L  E++RKE
Sbjct: 5   DYYQILGVSKDATAEEIKKAYRRLAKEYHPDISADENASEKFKEINEAYHILSDEERRKE 64

Query: 148 YD---SSMGETRVR-FGNYNPGLGYSSWKGPLRS 177
           YD    S  E   R F  Y      S W+G  RS
Sbjct: 65  YDRILKSGDEKSYRDFMEYIQDFLESIWQGMRRS 98

BLAST of Cp4.1LG01g23380 vs. ExPASy Swiss-Prot
Match: B9DNJ9 (Chaperone protein DnaJ OS=Staphylococcus carnosus (strain TM300) OX=396513 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.5e-10
Identity = 38/81 (46.91%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLR-LNEAYRVLMREDQRKE 147
           +YYE+LGVS D++  EIK+AYRKL KK+HPDI  +EG E   + ++EAY VL  E++R  
Sbjct: 5   DYYEVLGVSKDASKDEIKKAYRKLSKKYHPDINQEEGAEEKFKEISEAYEVLSDENKRAN 64

Query: 148 YDSSMGETRVRFGNYNPGLGY 168
           YD        +FG+  P  G+
Sbjct: 65  YD--------QFGHDGPQGGF 77

BLAST of Cp4.1LG01g23380 vs. ExPASy Swiss-Prot
Match: Q8G6C6 (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 4.2e-10
Identity = 36/85 (42.35%), Postives = 49/85 (57.65%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEY 147
           +YYE LGV   ++  EIK+AYRKL +K+HPDIAG E  ++   +N AY VL   D+R+ Y
Sbjct: 3   DYYETLGVERGASDDEIKKAYRKLSRKYHPDIAGPEFEDKFKEVNNAYDVLSNPDKRRMY 62

Query: 148 DSSMGETRVRFGNYNPGLGYSSWKG 173
           DS +          NP  G   + G
Sbjct: 63  DSGVDPN-------NPNAGAGGFSG 80

BLAST of Cp4.1LG01g23380 vs. ExPASy Swiss-Prot
Match: Q6A997 (Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) OX=267747 GN=dnaJ1 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 35/82 (42.68%), Postives = 50/82 (60.98%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEY 147
           +YYE+LGVS D++A EIK+AYR+   K HPD+AG    E   ++ EAY +L    +R  +
Sbjct: 4   DYYEILGVSHDASADEIKKAYRRKAMKLHPDVAGPGSEEEFKKVQEAYEILQDPQKRAVF 63

Query: 148 DSSMGETRVRFGNYNPGLGYSS 170
           D   G+   R G +  G G+SS
Sbjct: 64  DRG-GDPNARMGGFGEG-GFSS 83

BLAST of Cp4.1LG01g23380 vs. NCBI nr
Match: XP_023548490.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597 bits (1540), Expect = 1.13e-211
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF
Sbjct: 61  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 332
           QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR
Sbjct: 241 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 294

BLAST of Cp4.1LG01g23380 vs. NCBI nr
Match: XP_022989943.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 576 bits (1484), Expect = 3.98e-203
Identity = 283/292 (96.92%), Postives = 287/292 (98.29%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSV MASSSFAHPNEPKHIIRKPRWTA+RCSARERAN+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVSMASSSFAHPNEPKHIIRKPRWTAVRCSARERANETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMRED+RKEYDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDRRKEYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEGYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGT 330
           QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEK NGIWNWVKQV GT
Sbjct: 241 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKFNGIWNWVKQVLGT 292

BLAST of Cp4.1LG01g23380 vs. NCBI nr
Match: XP_022962168.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 575 bits (1483), Expect = 5.25e-203
Identity = 284/294 (96.60%), Postives = 287/294 (97.62%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSVPMASSSFAHPNE K I+RKPRWTAIRCSARER N+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVPMASSSFAHPNESKLIMRKPRWTAIRCSARERGNETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRK YDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKNYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEGYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 332
           QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIW WVKQVFGTHR
Sbjct: 241 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWKWVKQVFGTHR 294

BLAST of Cp4.1LG01g23380 vs. NCBI nr
Match: KAG7033102.1 (Chaperone protein dnaJ C76, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 572 bits (1474), Expect = 1.23e-201
Identity = 282/294 (95.92%), Postives = 286/294 (97.28%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSVPMAS SFAHPNE K I+RKPRWTAIRCSARERAN+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVPMASPSFAHPNESKLIMRKPRWTAIRCSARERANETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRK YDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKNYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGP RSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPQRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKERYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 332
           QQLKRQKQDTKFG ETVEETPAQAEARANASMKIQMEKI+GIWNWVKQVFGTHR
Sbjct: 241 QQLKRQKQDTKFGNETVEETPAQAEARANASMKIQMEKIDGIWNWVKQVFGTHR 294

BLAST of Cp4.1LG01g23380 vs. NCBI nr
Match: KAG6602425.1 (Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 571 bits (1472), Expect = 2.47e-201
Identity = 282/294 (95.92%), Postives = 286/294 (97.28%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSVPMAS SFAHPNE K I+RKPRWTAIRCSARERAN+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVPMASPSFAHPNESKLIMRKPRWTAIRCSARERANETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRK YDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKNYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGP RSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPQRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEGYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 332
           QQLKRQKQDTKFG ETVEETPAQAEARANASMKIQMEKI+GIWNWVKQVFGTHR
Sbjct: 241 QQLKRQKQDTKFGNETVEETPAQAEARANASMKIQMEKIDGIWNWVKQVFGTHR 294

BLAST of Cp4.1LG01g23380 vs. ExPASy TrEMBL
Match: A0A6J1JRT4 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111486979 PE=4 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 1.93e-203
Identity = 283/292 (96.92%), Postives = 287/292 (98.29%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSV MASSSFAHPNEPKHIIRKPRWTA+RCSARERAN+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVSMASSSFAHPNEPKHIIRKPRWTAVRCSARERANETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMRED+RKEYDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDRRKEYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEGYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGT 330
           QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEK NGIWNWVKQV GT
Sbjct: 241 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKFNGIWNWVKQVLGT 292

BLAST of Cp4.1LG01g23380 vs. ExPASy TrEMBL
Match: A0A6J1HE12 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462699 PE=4 SV=1)

HSP 1 Score: 575 bits (1483), Expect = 2.54e-203
Identity = 284/294 (96.60%), Postives = 287/294 (97.62%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAISIVPSIQRSVPMASSSFAHPNE K I+RKPRWTAIRCSARER N+TNYYELLGVSAD
Sbjct: 1   MAISIVPSIQRSVPMASSSFAHPNESKLIMRKPRWTAIRCSARERGNETNYYELLGVSAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           SNA+EIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRK YDSSMGE RVRF
Sbjct: 61  SNAQEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKNYDSSMGEMRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN
Sbjct: 121 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKE YGVFGGGWEIPANVFMAAKAFT
Sbjct: 181 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEGYGVFGGGWEIPANVFMAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVFGTHR 332
           QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIW WVKQVFGTHR
Sbjct: 241 QQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWKWVKQVFGTHR 294

BLAST of Cp4.1LG01g23380 vs. ExPASy TrEMBL
Match: A0A6J1C255 (chaperone protein dnaJ C76, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006725 PE=4 SV=1)

HSP 1 Score: 502 bits (1292), Expect = 3.32e-174
Identity = 246/293 (83.96%), Postives = 268/293 (91.47%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPNEPKHIIRKPRWTAIRCSARERANQTNYYELLGVSAD 98
           MAIS VPS+Q S+ MAS S AH NE  HI+R+ R TAIRCSARER+N+ NYYE+LGV AD
Sbjct: 1   MAISTVPSMQHSMAMASPSIAHSNEHNHILRRQRRTAIRCSARERSNEMNYYEVLGVPAD 60

Query: 99  SNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 158
           S  +EIKEAYRKLQKK+HPDIAGQEGHE TLRLNEAYRVLMREDQRKEYDSSMGETRVRF
Sbjct: 61  SKTQEIKEAYRKLQKKYHPDIAGQEGHEHTLRLNEAYRVLMREDQRKEYDSSMGETRVRF 120

Query: 159 GNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDN 218
           GNY+PGLGYSSWKGPLR QALFVDGNACIGCRECVHHASNTF MDETLGRARV+VQYGDN
Sbjct: 121 GNYSPGLGYSSWKGPLRPQALFVDGNACIGCRECVHHASNTFAMDETLGRARVQVQYGDN 180

Query: 219 DQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFT 278
           D+KIQVSVESCPVNCIYWV++EELAVLEYLMQPLPKE YGVFGGGWEIPANVF AAKAFT
Sbjct: 181 DKKIQVSVESCPVNCIYWVDREELAVLEYLMQPLPKEGYGVFGGGWEIPANVFAAAKAFT 240

Query: 279 QQLKRQKQDTKFGKETVEE--TPAQAEARANASMKIQMEKINGIWNWVKQVFG 329
           ++L+RQK DTK+G+ETVEE  TPAQA+ARANASM+IQ EK  G+WNWVKQVFG
Sbjct: 241 KRLERQKHDTKYGRETVEERETPAQAQARANASMEIQREKFYGVWNWVKQVFG 293

BLAST of Cp4.1LG01g23380 vs. ExPASy TrEMBL
Match: A0A0A0KUF2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G523160 PE=4 SV=1)

HSP 1 Score: 471 bits (1212), Expect = 4.75e-162
Identity = 238/298 (79.87%), Postives = 260/298 (87.25%), Query Frame = 0

Query: 39  MAISIVPSIQRSVP--MASSSFAHPNEP-KHIIR-----KPRWTAIRCSARERANQTNYY 98
           MAISIVPSI  S+P   +S S  HP +  KHI+R     + R T+IRCSA ER N+ NYY
Sbjct: 1   MAISIVPSIHHSMPPIASSPSLPHPKDHNKHILRILVGRRQRLTSIRCSASERVNEMNYY 60

Query: 99  ELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSS 158
           ELLGVSADSN +EIKEAYRKLQKKFHPDIAGQEGHE TLRLNEAY VLMRED+RK+YD+ 
Sbjct: 61  ELLGVSADSNTQEIKEAYRKLQKKFHPDIAGQEGHEYTLRLNEAYGVLMREDRRKQYDTY 120

Query: 159 MGETRVRFGNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRAR 218
            G  RVRFGN+  GLGYSSW GPLR QALFVDGNACIGCRECVH+ASNTFTMDETLGRAR
Sbjct: 121 TGGMRVRFGNFGTGLGYSSWNGPLRPQALFVDGNACIGCRECVHNASNTFTMDETLGRAR 180

Query: 219 VKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANV 278
           VKVQYGDND+KIQVSVESCPVNCIYWVEKEEL VLEYLMQPLPK+ YGVFGGGWEIPANV
Sbjct: 181 VKVQYGDNDKKIQVSVESCPVNCIYWVEKEELEVLEYLMQPLPKKGYGVFGGGWEIPANV 240

Query: 279 FMAAKAFTQQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWVKQVF 328
           FMAAKAFT+QL+RQ+QDTK+G ETV ETPAQAEARANA+MKIQ EK +G+WNWVKQVF
Sbjct: 241 FMAAKAFTKQLERQEQDTKYGAETVGETPAQAEARANATMKIQREKFHGVWNWVKQVF 298

BLAST of Cp4.1LG01g23380 vs. ExPASy TrEMBL
Match: A0A5A7T7Y1 (Chaperone protein DnaJ-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00530 PE=4 SV=1)

HSP 1 Score: 466 bits (1199), Expect = 2.16e-159
Identity = 241/340 (70.88%), Postives = 262/340 (77.06%), Query Frame = 0

Query: 39  MAISIVPSIQRSVPMASSSFAHPN---EPKHIIRKPRWTAIRCS---------------- 98
           MAISIVP +  S+P  +SS +HP+     KHI+R+ RWTAIRCS                
Sbjct: 1   MAISIVPCVHNSMPPIASSPSHPHPKGHSKHILRRQRWTAIRCSTSERANEMNYYAMPLM 60

Query: 99  -------------------------------ARERANQTNYYELLGVSADSNAKEIKEAY 158
                                          A ERAN+ NYYELLGVSADSNA+EIK AY
Sbjct: 61  TSTPSLAHHNDHNKYISRILVGRKQTAIRCSAGERANEMNYYELLGVSADSNAQEIKAAY 120

Query: 159 RKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDSSMGETRVRFGNYNPGLGYS 218
           RKLQKKFHPDIAGQEGHE TLRLNEAYRVLMRED+RK+YD+  G  RVRFGN+  GLGYS
Sbjct: 121 RKLQKKFHPDIAGQEGHEYTLRLNEAYRVLMREDRRKQYDTYSGGMRVRFGNFGTGLGYS 180

Query: 219 SWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDETLGRARVKVQYGDNDQKIQVSVES 278
           SW GPLR QALFVDGNACIGCRECVH+ASNTFTMD+TLGRARVKVQYGDNDQKIQVSVES
Sbjct: 181 SWNGPLRPQALFVDGNACIGCRECVHNASNTFTMDDTLGRARVKVQYGDNDQKIQVSVES 240

Query: 279 CPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWEIPANVFMAAKAFTQQLKRQKQDT 328
           CPVNCIYWVEKEEL VLEYLMQPLPK+ YGVFGGGWEIPANVFMAAKAFT+QLKRQ+QD 
Sbjct: 241 CPVNCIYWVEKEELEVLEYLMQPLPKKGYGVFGGGWEIPANVFMAAKAFTKQLKRQEQDK 300

BLAST of Cp4.1LG01g23380 vs. TAIR 10
Match: AT5G23240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 149.4 bits (376), Expect = 7.8e-36
Identity = 95/238 (39.92%), Postives = 130/238 (54.62%), Query Frame = 0

Query: 90  YELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERTLRLNEAYRVLMREDQRKEYDS 149
           Y+LLG+   S+  +IK AYR LQK+ HPDIAG  GH+  + LNEAY++L     R+ YD 
Sbjct: 52  YDLLGIDRSSDKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDK 111

Query: 150 SMGETRVRFGNYNPGLGYSSWKGPLRSQ-ALFVDGNACIGCRECVHHASNTFTMDETLGR 209
              +     G Y     YS W GP   Q A FVD   C+GC +C   A  TF ++   GR
Sbjct: 112 EQAKLEELRG-YTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGR 171

Query: 210 ARVKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLM--QPLPKEEYGVFGGGWEI 269
           ARV  Q+ D + KI+ ++E+CPV+CI  VE+ +LA LE+LM  QP      GV     E 
Sbjct: 172 ARVVAQWADPESKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGER 231

Query: 270 PANVFMAAKAFTQQLKRQKQDTKFGKETVEETPAQAEARANASMKIQMEKINGIWNWV 325
            +NVF+  K F      Q++  K    T +ET +Q E + +A     +E I  I NW+
Sbjct: 232 VSNVFVDVKKF------QERYAKAMSRTTKET-SQREVQISA-----VEAIRSISNWL 276

BLAST of Cp4.1LG01g23380 vs. TAIR 10
Match: AT2G42750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 96.7 bits (239), Expect = 6.0e-20
Identity = 68/220 (30.91%), Postives = 112/220 (50.91%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEGHERT--LRLNEAYRVLMREDQRK 147
           +YY +LG+  D+  +EIK+AY    K  HPD++G +       + +N+ Y +L    QR 
Sbjct: 76  DYYAVLGLLPDATQEEIKKAYYNCMKSCHPDLSGNDPETTNFCMFINDIYEILSDPVQRM 135

Query: 148 EYDSSMGETRVRFGNYNPGLGYSSWKGPLRSQALFVDGNACIGCRECVHHASNTFTMDET 207
            YD   G T       NP L  S+ +       +FVD  ACIGC+ C + A + F ++E 
Sbjct: 136 VYDEIHGYTVTAI---NPFLDDSTPR-----DHVFVDEFACIGCKNCANVAPDIFQIEED 195

Query: 208 LGRARVKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEYGVFGGGWE 267
            GRAR   Q G N   +Q +VE+CPV+CI+     +L++LE  M+ + +    +   G  
Sbjct: 196 FGRARACNQRG-NPDLVQQAVETCPVDCIHQTSAAQLSLLEDEMRRVERVNVALMLSGMG 255

Query: 268 IPA-NVFMAAKAFTQ----QLKRQKQDTKFGKETVEETPA 301
             A +VF  A++  +    ++  Q +     ++  +ETP+
Sbjct: 256 SGAVDVFRMARSRWEKRQAKVLNQARSRMMKRKNTDETPS 286

BLAST of Cp4.1LG01g23380 vs. TAIR 10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 7.3e-10
Identity = 47/173 (27.17%), Postives = 79/173 (45.66%), Query Frame = 0

Query: 90  YELLGVSADSNAKEIKEAYRKLQKKFHPDI--AGQEGHERTLRLNEAYRVLMREDQRKEY 149
           Y +LGV    ++ E+K A+R   K++HPD+   G        R+ +AY +L    + +  
Sbjct: 46  YSILGVEPSCSSSELKAAFRAKVKQYHPDVNKDGSNSDIMIRRIIQAYEMLTNYSRSEII 105

Query: 150 DSSMGETRVRFGNYNPGLGYSSWKGPLRSQALFVDGNACIGCR---ECVHHASNTFTMDE 209
           +   GE    F +               +  +FV+   C+G R    C   AS+ F+ D 
Sbjct: 106 E---GECLDPFDH-----------PECEALDVFVNEVLCVGKRCSYPCFETASHVFSCDS 165

Query: 210 TLGRARVKVQYGDNDQKIQVSVESCPVNCIYWVEKEELAVLEYLMQPLPKEEY 258
           + G AR   Q    D ++Q +V  CP NCI++V   +  +LE L+  +  + Y
Sbjct: 166 S-GTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPSQRIILEELLDSVVDKPY 203

BLAST of Cp4.1LG01g23380 vs. TAIR 10
Match: AT4G09350.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 4.0e-08
Identity = 27/69 (39.13%), Postives = 45/69 (65.22%), Query Frame = 0

Query: 88  NYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAG---QEGHERTLRLNEAYRVLMREDQR 147
           ++Y+ LGVS D++ +EIK AYR+L K++HPD      +   E+ ++L E Y VL  E+ R
Sbjct: 106 SHYQFLGVSTDADLEEIKSAYRRLSKEYHPDTTSLPLKTASEKFMKLREVYNVLSDEETR 165

Query: 148 KEYDSSMGE 154
           + YD ++ +
Sbjct: 166 RFYDWTLAQ 174

BLAST of Cp4.1LG01g23380 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.5e-07
Identity = 32/75 (42.67%), Postives = 45/75 (60.00%), Query Frame = 0

Query: 84  ANQTNYYELLGVSADSNAKEIKEAYRKLQKKFHPDIAGQEG-HERTLRLNEAYRVLMRED 143
           A   +YY  LGVS  +N KEIK AYR+L +++HPD+  + G  E+   ++ AY VL  E 
Sbjct: 71  ATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGATEKFKEISAAYEVLSDEQ 130

Query: 144 QRKEYDSSMGETRVR 158
           +R  YD   GE  V+
Sbjct: 131 KRALYD-QYGEAGVK 144

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMX61.1e-3439.92Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC... [more]
Q75VW31.3e-1145.74Chaperone protein DnaJ OS=Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12... [more]
B9DNJ92.5e-1046.91Chaperone protein DnaJ OS=Staphylococcus carnosus (strain TM300) OX=396513 GN=dn... [more]
Q8G6C64.2e-1042.35Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=... [more]
Q6A9971.2e-0942.68Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) O... [more]
Match NameE-valueIdentityDescription
XP_023548490.11.13e-211100.00chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022989943.13.98e-20396.92chaperone protein dnaJ C76, chloroplastic-like [Cucurbita maxima][more]
XP_022962168.15.25e-20396.60chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata][more]
KAG7033102.11.23e-20195.92Chaperone protein dnaJ C76, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
KAG6602425.12.47e-20195.92Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1JRT41.93e-20396.92chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HE122.54e-20396.60chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1C2553.32e-17483.96chaperone protein dnaJ C76, chloroplastic-like OS=Momordica charantia OX=3673 GN... [more]
A0A0A0KUF24.75e-16279.87J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G523160 PE=4 SV=1[more]
A0A5A7T7Y12.16e-15970.88Chaperone protein DnaJ-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT5G23240.17.8e-3639.92DNAJ heat shock N-terminal domain-containing protein [more]
AT2G42750.16.0e-2030.91DNAJ heat shock N-terminal domain-containing protein [more]
AT3G05345.17.3e-1027.17Chaperone DnaJ-domain superfamily protein [more]
AT4G09350.14.0e-0839.13Chaperone DnaJ-domain superfamily protein [more]
AT1G80030.11.5e-0742.67Molecular chaperone Hsp40/DnaJ family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 90..108
score: 48.23
coord: 108..123
score: 45.36
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 87..143
e-value: 4.2E-18
score: 76.1
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 88..148
e-value: 1.2E-16
score: 60.5
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 88..151
score: 17.588341
IPR001623DnaJ domainCDDcd06257DnaJcoord: 88..138
e-value: 1.55705E-15
score: 68.7261
NoneNo IPR availablePFAMPF13370Fer4_13coord: 181..235
e-value: 5.3E-10
score: 39.7
NoneNo IPR availableGENE3D3.30.70.20coord: 177..239
e-value: 8.6E-17
score: 63.1
NoneNo IPR availablePANTHERPTHR45295:SF43FE-4S FERREDOXINcoord: 65..331
NoneNo IPR availablePANTHERPTHR45295CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 65..331
NoneNo IPR availableSUPERFAMILY548624Fe-4S ferredoxinscoord: 179..238
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 75..160
e-value: 6.0E-21
score: 76.4
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 84..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g23380.1Cp4.1LG01g23380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding