Cp4.1LG01g22250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g22250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCytochrome b561 domain-containing protein
LocationCp4.1LG01: 20393378 .. 20397545 (+)
RNA-Seq ExpressionCp4.1LG01g22250
SyntenyCp4.1LG01g22250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATCCAAAAGTCAACGTAAAGCAAAACCTTTTTTTTTTTTTCTTTGAAATATTCTATACTAAAATTAAGCCTAAATTTCCATTCCATCTCACCAATGGCGATGAGTGATTCGAGATCTTAGGGAGAAATCAACGTCTGAAATTTCATTCGATCAGTGTTTTTCCTTAATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTATCTCATCAGCTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGGTTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATCTCAGATAACAAGGATCTCATCTTCAATGTAATTAGCAATTTCCTCTATATTCACAAATTATTTTGCTCTTGCGTGTGTGTTTTTTTTCCCTCTTGTTTCCGATTTCTGAATTGATGCGCATGTTCGATGGGTCTTGAGTTAATTTAGTTTTCCCTGGTCTTTCTGCTTCGGATTTTGGATTTGGATGTTTGAAATTTGAGCACCATTTGGCTTGTCACCAGCAGAAATGTTTGGAATGAAATTAACGGTGCTCACTACTTGCTCACCATTGTTAGTTCTTCTCAGCTTCAGCCTGAAATGGCTGTTTAATGTACTCCTGTAGTTTTGAATTGTATGCTCTAAACCCATTGATGAAGCGTAGAGTAATTGGGTCTATTCTTTCTGGGGTTTGGAATTTTCATGACAAATTTAGAGATGAATTTTGAATGGCTGATCATGTCTTGGGGGCAAAAGAAGTCATATGCATATAGACACCTTTTACTGTCAGCCATCTATGATCTGCATTTCTTATCAGTTTCAAAAAGATGGGAGATAATGCAAAATAGAATTCATTCTTTACATGTGGCTCATATCAAGGTCAATAGAATTTTTTGTTATTGACACAGCTTAGCTCGAGTAACATTTGGCTATCTACTATTATTTCTTGTGCCTCTTATATGAATCTAGTAACAAGCATGGTTCGTTCTTAGTATCTAAACTATTTTTTAATGCATATCTTCGTATAATTGTTTATTTTATATTTGATGGCAATCCTAGTGGAGAGTGTTTATTTGAGATCCCACATCGATTGGGGAGGAGAATGAAGCATTCTTTATAAGGGTATGGAAACCTCTCCCTAGCAGACGCATACGCATTTTAAAAACTTTGAGGGGAAGTCTGAAGAGAACAATATCTACTAGCAGTGGGCTTGGGCTGTTACAGTTTGTGTACTCCCGTTCGCTCCCATTCTGGGACTCTACTTAAATAATGCATCTGATGGTCTTGTAGTTTATAAGTTTGGTCTTGTAGTGGATAGAAACCAAGGATATGAGCATAATATTGAAATGGTATTGTCATGAAACTTTTGGATCTAATGATGTCACGCGAGGGAAACATGATTATTATTTTTGCTGTTGATATATTCTATATGCTTTACATAATAGAAACTTTAATTTTTAATTCTTTTTGTCCTTTTATTTTCCTGTTTATTTGGTGAAGTTGCTCCTGTTTTGTAGGTCCATCCTGTTCTTATGGTGATTGGACTTATACTCCTAAATGGTGAAGGTAAATATCTTTTTTCTTTGGACTTAAATTATAGTTTGAAGCACTAGTTTACACACGTTTGGATTCTTCTTCCATTTCGGTTTTGATGCGATGTTTTGTATTTGACTATTTGAATGCATATGTAGGCTGTGAAAATATAATGCTACTATGTTAAAAGTGAGAAATCACTTTTTTATGAGAAACAAACTGCATTTGATAGGAGATATGAAATTACAAAAAAATAAGGGGGGAGGAAAGCCAAATCACATTTGATATAAATAAGCGTTTTTGTGGGCTACGGGTGGAGGGTTATGAGATCGTCACTCAATCCTGGGGAAAGAACTCCCTGAGCCTTAGCTGGAACCTAAACCAAAGCTAAGCTTGTAGGCTCGTAGAGAAAGTCATTTTACAGGTATACCCACGGATCTATGTTCCTGAACAAGCCAAGAAAATTATGAAAACCTCTTTCAGTTGGCACAAAGATAAGAAGGTTGTAAAAAAAAAAAAAGGCTCTTACTAAAGAGCAAGTATTCTTTTGCCTTTCTCTAAAGAGAAATAACAGAGTTGGAGAGTTGGAACTAGTAGATTGTAATTTAAGTGGCTCTTTTGATCTAGAACATTCAGTGTGCTCAATTTTTTCTTCCCTGGGTGGTGGGGTGTAGAGTTGTATGAGAGAGATTGGTACTGTTGTTACTAGTTACTACTGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGAGAAAACTGGGATAATGGGCAAGGATCTTAACAGTATAAAACATTTGCTAGGCTTTGTCATGTAGGTCTTAGGTTTTGTACAGGAAACGTGTTATTTTACGAAACGTGCTGGAGGTCAGGTTTAGAATCATGTATGAAATATGTATCCTGAAATCATTCACAAACTTGAGTTAAAGTTAATTCTCCTACCATTCTGGTGCGTTCAATGGTTCTGTTCACCGTGCTTAGATGTATTGATGCAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTTCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGGTTCGTAAATTGCAATTTCTGCTGGTGTGATGGCATGATTTACTTTTGAGCCCGCTTGTTGAGCTATAACTCATTAGGATGACGCCATTTTAACAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCCGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTCAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAGTAGCAAAGGCGACATTTTCAGGGGGCCAGCTGAATAGGATAAAGAAGCCAATGGAAATGGAACAAGGATTTTTGTTGCGTGACGGATCTTTCCTGCTGTAAACATATCATAATCGAACTTGCCTTTATTTTTGACTCGAGACAGTTAGATTCTTCCATAACACAAAAATCACATCATAGGTTGAGAAGCAAACAGATTCTTTAGTTGAGAATAAGATGTTATATGTTGTCTTTTCACGTGGAGCTATACTCATTTTGGCAAAATTTAAGCTTTGATTTTGAGCAGATCGAAGCTAAGAAAATTTATAGTACAATGATATTACACCTTCAATTTTCATTAAAAAAAAAACGTTAACACATACGTGCAACGTTATTTTATTTGATGGATGTCATACCATTCAAATACTAGCAAAATATCAATATTGAAAAAAAAAAAAAATCCAAACCAAGTTTAGAAAAGTTCATATCTAGTAGTTCGGTCAATTTCAAAAAAGTTAAATATTTTAGTACTGGAAGTTTAGAAACATTCAAAATCAGCCAACACTTATAGCAAGAAAGCTATAGTTCACTATTTATAAGAAATCAGATAAAAAGTTGGGATAGCAAACAGATCATAGAACTATGAAAGCTAAAAGTTCAACTGATAAGAAGAAAACATGTCATGACCAACAAACAAACAGATGTGCCAATAAAAAGAGGGGTAAACTATGGCAATGATATGGCTGTCTGATGGACTGTCTTCTGGATGTAGCGGCCATTTGATTACATGAAACTTGGGCAAACAGGTCCTTTGGAAAGACCAAGGCGGACTTCCATTCCATGGTGCATGTCCAATGGCCTCAAAGATTCAGACTCCCTTGGGTCTGCAAATAACATTGACATAGGAAGTTGTAAATAAACACCAGCAGAGACTATTATCTAAATGGGTATATATAGACTCATGAAAACACGGCGATATAGGACTAGAA

mRNA sequence

TAATCCAAAAGTCAACGTAAAGCAAAACCTTTTTTTTTTTTTCTTTGAAATATTCTATACTAAAATTAAGCCTAAATTTCCATTCCATCTCACCAATGGCGATGAGTGATTCGAGATCTTAGGGAGAAATCAACGTCTGAAATTTCATTCGATCAGTGTTTTTCCTTAATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTATCTCATCAGCTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGGTTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATCTCAGATAACAAGGATCTCATCTTCAATGTCCATCCTGTTCTTATGGTGATTGGACTTATACTCCTAAATGGTGAAGTTAATTCTCCTACCATTCTGGTGCGTTCAATGGTTCTGTTCACCGTGCTTAGATGTATTGATGCAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTTCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCCGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTCAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAGTAGCAAAGGCGACATTTTCAGGGGGCCAGCTGAATAGGATAAAGAAGCCAATGGAAATGGAACAAGGATTTTTGTTGCGTGACGGATCTTTCCTGCTGTAAACATATCATAATCGAACTTGCCTTTATTTTTGACTCGAGACAGTTAGATTCTTCCATAACACAAAAATCACATCATAGGTTGAGAAGCAAACAGATTCTTTAGTTGAGAATAAGATGTTATATGTTGTCTTTTCACGTGGAGCTATACTCATTTTGGCAAAATTTAAGCTTTGATTTTGAGCAGATCGAAGCTAAGAAAATTTATAGTACAATGATATTACACCTTCAATTTTCATTAAAAAAAAAACGTTAACACATACGTGCAACGTTATTTTATTTGATGGATGTCATACCATTCAAATACTAGCAAAATATCAATATTGAAAAAAAAAAAAAATCCAAACCAAGTTTAGAAAAGTTCATATCTAGTAGTTCGGTCAATTTCAAAAAAGTTAAATATTTTAGTACTGGAAGTTTAGAAACATTCAAAATCAGCCAACACTTATAGCAAGAAAGCTATAGTTCACTATTTATAAGAAATCAGATAAAAAGTTGGGATAGCAAACAGATCATAGAACTATGAAAGCTAAAAGTTCAACTGATAAGAAGAAAACATGTCATGACCAACAAACAAACAGATGTGCCAATAAAAAGAGGGGTAAACTATGGCAATGATATGGCTGTCTGATGGACTGTCTTCTGGATGTAGCGGCCATTTGATTACATGAAACTTGGGCAAACAGGTCCTTTGGAAAGACCAAGGCGGACTTCCATTCCATGGTGCATGTCCAATGGCCTCAAAGATTCAGACTCCCTTGGGTCTGCAAATAACATTGACATAGGAAGTTGTAAATAAACACCAGCAGAGACTATTATCTAAATGGGTATATATAGACTCATGAAAACACGGCGATATAGGACTAGAA

Coding sequence (CDS)

ATGCAGCTGAAATTGGCTTCGAATTTGCCCACGATTTCCTCCATTGAAGATCCTAATACTCCAATTCTTTCCCCCACTATCTCATCAGCTGAAGAACCAATGGCGGTTCCCGCGGTTCAGTTCCCGATCTTCATGGCCGTGAGAGTGCTCGGGATGGTTGTGGCAACTCTCGTCTTCATTTGGACCTTGCACTTCCGTGGAGGATTGGCGCTTATCTCAGATAACAAGGATCTCATCTTCAATGTCCATCCTGTTCTTATGGTGATTGGACTTATACTCCTAAATGGTGAAGTTAATTCTCCTACCATTCTGGTGCGTTCAATGGTTCTGTTCACCGTGCTTAGATGTATTGATGCAGCAATGTTGGCATACAAGACAGTCTCAGGAACCAAAAATTTCAGAAAACTAGTTCATCTTGCAGTACAGTTTGTTGCATTCTGTTTAAGCCTGATTGGCATATGGGCTGCCCTGAAGTTTCACAACGACAAAGGAATCGAGAACTTTTACAGTTTGCATTCATGGTTGGGTCTAGTTTCGATCTTTCTGTTCGGCTTCCAGTGGACTGCAGGATTTGTAACCTTTTGGTACCCAGGCGGCTCCAGAAACAGCCGATCGACTCTATTGCCATGGCATGTTTTCTTTGGTGTTTACATATATGGCCTTTCCATTGCTACAGCTGTTACTGGCTTGTTAGAAAAAGCTACATTTCTTCAAGTCAGCAAGGTGATTTTACGCTATTCCAACGAGGCGTTGCTGTTAAATTCGTTGGGTCTCCTCATTGTTCTTCTGGGTGGTCTTGTAATTCTCGCGGTTATCGCTCCATCGAGTAGCAAAGGCGACATTTTCAGGGGGCCAGCTGAATAG

Protein sequence

MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE
Homology
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BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match: Q9SWS1 (Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB561B PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.7e-85
Identity = 160/249 (64.26%), Postives = 190/249 (76.31%), Query Frame = 0 

Query: 34  MAVPAV-QFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLI 93
           MAVP +  FPIFM VRVLG ++A LV  WT+H+RGGLAL SDNKD IFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 94  LLNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIG 153
           L NGE                      AMLAYK+V GTKN +KLVHL +Q  AF LSLIG
Sbjct: 61  LFNGE----------------------AMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIG 120

Query: 154 IWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHV 213
           +WAALKFH DKGIENFYSLHSWLGL  +FLF FQW AGFVT+WYPGGSRNSR++L+PWHV
Sbjct: 121 VWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHV 180

Query: 214 FFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVI 273
           F G+ IY L++ TA TG+LEK TFLQV++VI RYS EA+L+N++G+LI++LGG VIL V+
Sbjct: 181 FLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVV 227

Query: 274 APSSSKGDI 282
            P S K  +
Sbjct: 241 TPVSGKDQV 227

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BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 3.1e-47
Identity = 104/235 (44.26%), Postives = 141/235 (60.00%), Query Frame = 0 

Query: 42  PIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSP 101
           P       L +  A +V +W + FRGGLA  +DNK+LIFNVHPVLM+IG I+L  E    
Sbjct: 11  PFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSE---- 70

Query: 102 TILVRSMVLFTVLRCIDAAMLAYKTVSG-TKNFRKLVHLAVQFVAFCLSLIGIWAALKFH 161
                             A++ YK       +  KL+HL +  +A  L  +GI+ A KFH
Sbjct: 71  ------------------AIMIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFH 130

Query: 162 NDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYG 221
           N+ GI N YSLHSWLG+ +I L+G QW  GFV F+YPG + + R   LPWHV FG+++Y 
Sbjct: 131 NESGIANLYSLHSWLGIGTISLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYV 190

Query: 222 LSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS 276
           L++ATA  GLLEK TFLQ S  + +Y  EA L+N  GL++ L G  V++A +AP+
Sbjct: 191 LTLATAELGLLEKLTFLQ-SSGLDKYGAEAFLVNFTGLVVALFGAAVVVAAVAPA 222

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BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.5e-46
Identity = 101/235 (42.98%), Postives = 142/235 (60.43%), Query Frame = 0 

Query: 42  PIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSP 101
           P       L +  AT+V +W +HFRGGLA  + NK+LIFNVHPVLM+IG I+L  E    
Sbjct: 9   PFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSE---- 68

Query: 102 TILVRSMVLFTVLRCIDAAMLAYKTVSGTK-NFRKLVHLAVQFVAFCLSLIGIWAALKFH 161
                             A++ YK +   K +  KL+HL +  +A     +GI+ A KFH
Sbjct: 69  ------------------AIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFH 128

Query: 162 NDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYG 221
           N+ GI N YSLHSWLG+ +I L+G QW  GFV F++P  S + R  +LPWH+ FG+++Y 
Sbjct: 129 NESGIANLYSLHSWLGIGTICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYI 188

Query: 222 LSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS 276
           L++ATA  G LEK TFLQ S  + +Y  EA L+N   L++VL G  V++A ++P+
Sbjct: 189 LALATAELGFLEKLTFLQ-SSGLDKYGAEAFLVNFTALIVVLFGASVVVAAVSPA 220

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BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 99/234 (42.31%), Postives = 144/234 (61.54%), Query Frame = 0 

Query: 42  PIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSP 101
           P   A   L +  A +V +W++ FRGGLA+ S NK+LIFNVHPVLM+IG +++ GE    
Sbjct: 11  PFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGE---- 70

Query: 102 TILVRSMVLFTVLRCIDAAMLAYKTV-SGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFH 161
                             A++ Y+ + +   +  KL+HL +  +A  L  +GI+ A K H
Sbjct: 71  ------------------AIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNH 130

Query: 162 NDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYG 221
           N+ GI N YSLHSW+G+ +I L+G QW  GFVTF++PG + N +  +LPWHV FG+++Y 
Sbjct: 131 NESGIANLYSLHSWIGIGTITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYI 190

Query: 222 LSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAP 275
           L++A A  G LEK TFL+ S  + +Y  EA L+N   L++VL G  V++A IAP
Sbjct: 191 LALANAELGFLEKLTFLE-SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 98/234 (41.88%), Postives = 143/234 (61.11%), Query Frame = 0 

Query: 42  PIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSP 101
           P   A   L +  A +V +W ++FRGGLA+ + NK+LIFNVHPVLM+IG I++ GE    
Sbjct: 11  PFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGE---- 70

Query: 102 TILVRSMVLFTVLRCIDAAMLAYKTV-SGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFH 161
                             A++ Y+ + +      KL+HL +  +A  L  +GI+ A K H
Sbjct: 71  ------------------AIMVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNH 130

Query: 162 NDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYG 221
           N+ GI N YSLHSW+G+ +I L+G QW  GFVTF++PG + N +  +LPWH+ FG+++Y 
Sbjct: 131 NESGIANLYSLHSWIGIGTITLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYI 190

Query: 222 LSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIAP 275
           L++A A  G LEK TFL+ S  + +Y  EA L+N   L++VL G  V++A IAP
Sbjct: 191 LALANAELGFLEKLTFLE-SSGLDKYGTEAFLVNFTALVVVLFGASVVVAAIAP 221

[Close]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match: XP_023541096.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497 bits (1279), Expect = 2.50e-176
Identity = 265/287 (92.33%), Postives = 265/287 (92.33%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match: XP_022989930.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita maxima])

HSP 1 Score: 493 bits (1269), Expect = 8.34e-175
Identity = 264/287 (91.99%), Postives = 264/287 (91.99%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTISSAEE MAVPAVQFPIFMAVRVLGMVVATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEELMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match: XP_022956458.1 (probable transmembrane ascorbate ferrireductase 2 [Cucurbita moschata])

HSP 1 Score: 490 bits (1262), Expect = 9.72e-174
Identity = 260/287 (90.59%), Postives = 263/287 (91.64%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTISS EEPMAVPAVQFPIFMAVRVLGMVVATLVF+
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFM 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match: KAG7033242.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 489 bits (1260), Expect = 1.89e-173
Identity = 260/286 (90.91%), Postives = 262/286 (91.61%), Query Frame = 0 

Query: 2   QLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFIW 61
           QLKLASNLPTISSIEDPNTPILSPTISS EEPMAVPAVQFPIFMAVRVLGMVVATLVFIW
Sbjct: 1   QLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFIW 60

Query: 62  TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAAM 121
           TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      AM
Sbjct: 61  TLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------AM 120

Query: 122 LAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIF 181
           LAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIF
Sbjct: 121 LAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSIF 180

Query: 182 LFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSK 241
           LFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSK
Sbjct: 181 LFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVSK 240

Query: 242 VILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           VILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 VILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 264

[Close]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match: KAG6602565.1 (putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488 bits (1257), Expect = 5.62e-173
Identity = 259/287 (90.24%), Postives = 262/287 (91.29%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPT SS EEPMAVPAVQFPIFMAVRVLGM+VATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTNSSTEEPMAVPAVQFPIFMAVRVLGMIVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match: A0A6J1JNQ1 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111486970 PE=4 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 4.04e-175
Identity = 264/287 (91.99%), Postives = 264/287 (91.99%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTISSAEE MAVPAVQFPIFMAVRVLGMVVATLVFI
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEELMAVPAVQFPIFMAVRVLGMVVATLVFI 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match: A0A6J1GWW7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita moschata OX=3662 GN=LOC111458185 PE=4 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 4.71e-174
Identity = 260/287 (90.59%), Postives = 263/287 (91.64%), Query Frame = 0 

Query: 1   MQLKLASNLPTISSIEDPNTPILSPTISSAEEPMAVPAVQFPIFMAVRVLGMVVATLVFI 60
           MQLKLASNLPTISSIEDPNTPILSPTISS EEPMAVPAVQFPIFMAVRVLGMVVATLVF+
Sbjct: 1   MQLKLASNLPTISSIEDPNTPILSPTISSTEEPMAVPAVQFPIFMAVRVLGMVVATLVFM 60

Query: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTILVRSMVLFTVLRCIDAA 120
           WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE                      A
Sbjct: 61  WTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGE----------------------A 120

Query: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180
           MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI
Sbjct: 121 MLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKGIENFYSLHSWLGLVSI 180

Query: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240
           FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS
Sbjct: 181 FLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIATAVTGLLEKATFLQVS 240

Query: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSSSKGDIFRGPAE 287
           KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPS+ KGDIFRGP+E
Sbjct: 241 KVILRYSNEALLLNSLGLLIVLLGGLVILAVIAPSNGKGDIFRGPSE 265

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match: A0A6J1BZR9 (probable transmembrane ascorbate ferrireductase 2 OS=Momordica charantia OX=3673 GN=LOC111006724 PE=4 SV=1)

HSP 1 Score: 403 bits (1036), Expect = 3.81e-140
Identity = 207/254 (81.50%), Postives = 221/254 (87.01%), Query Frame = 0 

Query: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVP VQFPIFMAVRVLGM+VATLV IWT HFRGGLALISDNKDLIFNVHPVLMVIGL+L
Sbjct: 1   MAVPVVQFPIFMAVRVLGMIVATLVIIWTSHFRGGLALISDNKDLIFNVHPVLMVIGLVL 60

Query: 94  LNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGI 153
           LNGE                      AMLAYKT SGTKNFRKLVHL++QFVAFCLSLIG+
Sbjct: 61  LNGE----------------------AMLAYKTASGTKNFRKLVHLSLQFVAFCLSLIGV 120

Query: 154 WAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVF 213
           WAALKFHNDKGI+NFYSLHSWLGL+S+FLFG QWTAGFVTFWYPGGSRNSR+TLLPWHVF
Sbjct: 121 WAALKFHNDKGIDNFYSLHSWLGLLSVFLFGIQWTAGFVTFWYPGGSRNSRATLLPWHVF 180

Query: 214 FGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIA 273
           FGVY YGLSIATA TGLLEKATFLQ SKVILRYSNEALL+NSLG+L+VLLGGLVILAVIA
Sbjct: 181 FGVYTYGLSIATAATGLLEKATFLQTSKVILRYSNEALLINSLGILLVLLGGLVILAVIA 232

Query: 274 PSSSKGDIFRGPAE 287
           P + KG+I RGPAE
Sbjct: 241 PPNGKGEILRGPAE 232

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match: A0A6J1K1L7 (probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN=LOC111489314 PE=4 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 2.56e-138
Identity = 207/254 (81.50%), Postives = 222/254 (87.40%), Query Frame = 0 

Query: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVPAVQFPIFMAVRVLGMVVA LVFIWTLHFRGGLAL SDNKDLIFNVHPVLMVIGLIL
Sbjct: 1   MAVPAVQFPIFMAVRVLGMVVAALVFIWTLHFRGGLALSSDNKDLIFNVHPVLMVIGLIL 60

Query: 94  LNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGI 153
           LNGE                      AMLAYKTVSGTKNF+KLVHLA+QFVA  LSLIGI
Sbjct: 61  LNGE----------------------AMLAYKTVSGTKNFKKLVHLALQFVALFLSLIGI 120

Query: 154 WAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVF 213
           WAALKFHNDKGI+NFYSLHSWLGLVS+FLFG QW AGFVTFWYPGGSRNSR+TLLPWHVF
Sbjct: 121 WAALKFHNDKGIDNFYSLHSWLGLVSVFLFGIQWGAGFVTFWYPGGSRNSRATLLPWHVF 180

Query: 214 FGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIA 273
           FGVYIYGL IAT VTGLLEKATFLQ SKVILRYSNEALL+NSLG+++V+LGGL+ILAVIA
Sbjct: 181 FGVYIYGLCIATTVTGLLEKATFLQTSKVILRYSNEALLINSLGIVVVVLGGLIILAVIA 232

Query: 274 PSSSKGDIFRGPAE 287
            S++KGD++RGPAE
Sbjct: 241 RSNNKGDVYRGPAE 232

[Close]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match: Q3LGX4 (Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 5.16e-138
Identity = 209/254 (82.28%), Postives = 223/254 (87.80%), Query Frame = 0 

Query: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAVP VQFPIFMAVRVLGM+VA L+FIWTLHFRGGLALIS+NKD IFNVHPVLMVIGLIL
Sbjct: 1   MAVPLVQFPIFMAVRVLGMLVAALLFIWTLHFRGGLALISENKDHIFNVHPVLMVIGLIL 60

Query: 94  LNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGI 153
           LNGE                      AMLAYKTVSGTKNF+KLVHLA+QFVAF LSLIGI
Sbjct: 61  LNGE----------------------AMLAYKTVSGTKNFKKLVHLALQFVAFFLSLIGI 120

Query: 154 WAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVF 213
           WAALKFHNDKGI+NFYSLHSWLGLVSIFLFG QW+AGFVTFWYPGGSRNSR+TLLPWHVF
Sbjct: 121 WAALKFHNDKGIDNFYSLHSWLGLVSIFLFGIQWSAGFVTFWYPGGSRNSRATLLPWHVF 180

Query: 214 FGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIA 273
           FG+YIYGLSIAT VTGLLEKATFLQ SKVILRYSNEALL+NSLG+LIVLLGGLVILAVIA
Sbjct: 181 FGIYIYGLSIATTVTGLLEKATFLQTSKVILRYSNEALLINSLGVLIVLLGGLVILAVIA 232

Query: 274 PSSSKGDIFRGPAE 287
           PS++KGD+ RG AE
Sbjct: 241 PSNAKGDVNRGLAE 232

[Close]
BLAST of Cp4.1LG01g22250 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 316.2 bits (809), Expect = 2.6e-86
Identity = 160/249 (64.26%), Postives = 190/249 (76.31%), Query Frame = 0 

Query: 34  MAVPAV-QFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLI 93
           MAVP +  FPIFM VRVLG ++A LV  WT+H+RGGLAL SDNKD IFNVHPV+MVIGLI
Sbjct: 1   MAVPVLGGFPIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLI 60

Query: 94  LLNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIG 153
           L NGE                      AMLAYK+V GTKN +KLVHL +Q  AF LSLIG
Sbjct: 61  LFNGE----------------------AMLAYKSVQGTKNLKKLVHLTLQLTAFILSLIG 120

Query: 154 IWAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHV 213
           +WAALKFH DKGIENFYSLHSWLGL  +FLF FQW AGFVT+WYPGGSRNSR++L+PWHV
Sbjct: 121 VWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSRASLMPWHV 180

Query: 214 FFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVI 273
           F G+ IY L++ TA TG+LEK TFLQV++VI RYS EA+L+N++G+LI++LGG VIL V+
Sbjct: 181 FLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILGGFVILGVV 227

Query: 274 APSSSKGDI 282
            P S K  +
Sbjct: 241 TPVSGKDQV 227

[Close]
BLAST of Cp4.1LG01g22250 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 180.6 bits (457), Expect = 1.7e-45
Identity = 97/244 (39.75%), Postives = 143/244 (58.61%), Query Frame = 0 

Query: 34  MAVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLIL 93
           MAV      +      L ++ A +V +W++ +RGGLA  + NK+LIFN+HPVLM+IG I+
Sbjct: 1   MAVRINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFII 60

Query: 94  LNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGI 153
           L GE                      A+++YK++   K  +KL+HL +  +A  L + GI
Sbjct: 61  LGGE----------------------AIISYKSLPLEKPVKKLIHLILHAIALALGIFGI 120

Query: 154 WAALKFHNDKGIENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVF 213
            AA K HN+  I N YSLHSW+G+  I L+GFQW   F+ F++PGGS N +S LLPWH  
Sbjct: 121 CAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAM 180

Query: 214 FGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVIA 273
            G+++Y L++  A  G LEK TFL+ +  + +Y +EA L+N   ++ +L G  V+L   A
Sbjct: 181 LGLFVYILAVGNAALGFLEKLTFLE-NGGLDKYGSEAFLINFTAIITILFGAFVVLTASA 221

Query: 274 PSSS 278
            S S
Sbjct: 241 ESPS 221

[Close]
BLAST of Cp4.1LG01g22250 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 101/242 (41.74%), Postives = 144/242 (59.50%), Query Frame = 0 

Query: 35  AVPAVQFPIFMAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFN-VHPVLMVIGLIL 94
           +V   +  + +  R+ G+VVA  V  W        AL   N  L ++ +HP+LMVIG IL
Sbjct: 3   SVDPSRLSLVLFARLSGLVVAVSVLYW--------ALFLPNLGLSYSTLHPLLMVIGFIL 62

Query: 95  LNGEVNSPTILVRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGI 154
           ++GE                      A+L ++ + G++  +K VHL +Q +A   ++ GI
Sbjct: 63  VSGE----------------------AILIHRWLPGSRKTKKAVHLWLQGMALASAVFGI 122

Query: 155 WAALKFHNDKGI-ENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHV 214
           W   KFH  +G+  NFYSLHSW+GL+S+ LF  QW  GF++FW+ G  R +R+T LPWHV
Sbjct: 123 WT--KFHYQRGVFANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTTRTTFLPWHV 182

Query: 215 FFGVYIYGLSIATAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAVI 274
           F G+Y YGL+IATA TGLLEK TFLQ  + + R  +E++ +N LGL + LLG +VI A I
Sbjct: 183 FLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALLGCIVITAAI 212

[Close]
BLAST of Cp4.1LG01g22250 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-30
Identity = 77/227 (33.92%), Postives = 116/227 (51.10%), Query Frame = 0 

Query: 45  MAVRVLGMVVATLVFIWTLHFRGGLALISDNKDLIFNVHPVLMVIGLILLNGEVNSPTIL 104
           +     G++   L+ IW LH+R G+   SDN   + NVHP LM  G + L G+       
Sbjct: 19  LVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQ------- 78

Query: 105 VRSMVLFTVLRCIDAAMLAYKTVSGTKNFRKLVHLAVQFVAFCLSLIGIWAALKFHNDKG 164
                          AM+ YKT   +   +K+VH  +  +   L ++GI AA +FH+   
Sbjct: 79  ---------------AMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVN 138

Query: 165 IENFYSLHSWLGLVSIFLFGFQWTAGFVTFWYPGGSRNSRSTLLPWHVFFGVYIYGLSIA 224
           +++  SLHSW+GL +  L G QW  G  TF  P  S  +R+ ++PWHV  G  +  + I 
Sbjct: 139 LKDMVSLHSWIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIV 198

Query: 225 TAVTGLLEKATFLQVSKVILRYSNEALLLNSLGLLIVLLGGLVILAV 272
            A+TGL+++AT L  S      + E+ L+N LGL I+L G  V  +V
Sbjct: 199 AALTGLMQRATMLGQST-----NAESRLINFLGLAILLFGVSVDFSV 218

The following BLAST results are available for this feature:
BLAST of Cp4.1LG01g22250 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SWS13.7e-8564.26Transmembrane ascorbate ferrireductase 2 OS=Arabidopsis thaliana OX=3702 GN=CYB5... [more]
Q7XMK33.1e-4744.26Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa... [more]
A3A9H61.5e-4642.98Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa... [more]
Q6I6812.6e-4642.31Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z... [more]
C4IYS82.6e-4641.88Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2... [more]
BLAST of Cp4.1LG01g22250 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023541096.12.50e-17692.33probable transmembrane ascorbate ferrireductase 2 [Cucurbita pepo subsp. pepo][more]
XP_022989930.18.34e-17591.99probable transmembrane ascorbate ferrireductase 2 [Cucurbita maxima][more]
XP_022956458.19.72e-17490.59probable transmembrane ascorbate ferrireductase 2 [Cucurbita moschata][more]
KAG7033242.11.89e-17390.91putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosper... [more]
KAG6602565.15.62e-17390.24putative transmembrane ascorbate ferrireductase 2, partial [Cucurbita argyrosper... [more]
BLAST of Cp4.1LG01g22250 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JNQ14.04e-17591.99probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1GWW74.71e-17490.59probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1BZR93.81e-14081.50probable transmembrane ascorbate ferrireductase 2 OS=Momordica charantia OX=3673... [more]
A0A6J1K1L72.56e-13881.50probable transmembrane ascorbate ferrireductase 2 OS=Cucurbita maxima OX=3661 GN... [more]
Q3LGX45.16e-13882.28Cytochrome b561 OS=Citrullus lanatus OX=3654 GN=CLb561B PE=2 SV=1[more]
BLAST of Cp4.1LG01g22250 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G38630.12.6e-8664.26cytochrome B561-1 [more]
AT4G25570.11.7e-4539.75Cytochrome b561/ferric reductase transmembrane protein family [more]
AT1G26100.11.2e-4141.74Cytochrome b561/ferric reductase transmembrane protein family [more]
AT1G14730.12.1e-3033.92Cytochrome b561/ferric reductase transmembrane protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006593Cytochrome b561/ferric reductase transmembraneSMARTSM00665561_7coord: 82..230
e-value: 1.1E-40
score: 151.2
IPR006593Cytochrome b561/ferric reductase transmembranePFAMPF03188Cytochrom_B561coord: 110..236
e-value: 6.2E-38
score: 130.1
IPR006593Cytochrome b561/ferric reductase transmembranePROSITEPS50939CYTOCHROME_B561coord: 46..272
score: 40.050629
NoneNo IPR availableGENE3D1.20.120.1770coord: 34..284
e-value: 8.0E-85
score: 285.6
NoneNo IPR availablePANTHERPTHR10106:SF49B561, PUTATIVE-RELATEDcoord: 101..283
coord: 34..98
NoneNo IPR availableCDDcd08766Cyt_b561_ACYB-1_likecoord: 73..238
e-value: 5.61292E-73
score: 218.715
IPR043205Cytochrome b561/Cytochrome b reductase 1-likePANTHERPTHR10106CYTOCHROME B561-RELATEDcoord: 101..283
coord: 34..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g22250.1Cp4.1LG01g22250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity