Cp4.1LG01g22200 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g22200
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING/U-box superfamily protein
LocationCp4.1LG01: 20309288 .. 20310583 (+)
RNA-Seq ExpressionCp4.1LG01g22200
SyntenyCp4.1LG01g22200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTTTCTCTATTCCTAAACTTCATCTTCAACCTAACATGCCTCTTCCTGATCAACCCCGCTCCGATTCTCCTTCTCCGGTGCCGTCGATCACATCCTTCCGCCGCGACGAATTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCGCTCCTCCCGACGATTCCCTCCGTTTTGAGACCGATTGGTTCTCCGACACGGATTCCGACTCCGAACGCGATGTCAGTTGCTTGGTGACTGATCTTTTTGAGAATCATCGCTTCACCGATCATTTACTCGCTCTCAGCAATCACGATCGTGATCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCAAACGACTTGGGCATTTCGGGGTTTGAAGATAGCGGTGACATTGAGTCTAACTATACTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGATTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAACAGTGACCTTCGGGTTGTTGATGTAGATTCGGACTCGGATTATGATAATGGAATTATCGGTGCTCTGCAATTTGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCAGATAACTTCAGCTTTTGTTTTCAGCAGAGGAATTCGTACGAGGATTTTGAATGGGAGGAGGTTGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTGGTGGAGGGAGCCGAGGAATTGACCCTTGCATCTGGTTTTTCCAATGATGAAGAATCAGGAGAAGAGGCGGGCCGATGGGAAATTTTATTGGTAATGGACGACATTGGGAGCAATACGAATCGGGAACGGGATAATGATGCTGAGGCGTACGTAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAACGCTTTGAAGGGTTGTCCTCCAGCAGCTAAGAGTGCTATGGAGAATCTTCCTCTGGTTGAGTTAAAAACAGAGAATATACTGACAGAGGAAGTGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCTTGCTGCCACTATTACCATGATGACTGCATTGTGCAATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCCACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAGAGGGCCAGTGGTGGCCAGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAA

mRNA sequence

ATGGCTTTTTTCTCTATTCCTAAACTTCATCTTCAACCTAACATGCCTCTTCCTGATCAACCCCGCTCCGATTCTCCTTCTCCGGTGCCGTCGATCACATCCTTCCGCCGCGACGAATTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCGCTCCTCCCGACGATTCCCTCCGTTTTGAGACCGATTGGTTCTCCGACACGGATTCCGACTCCGAACGCGATGTCAGTTGCTTGGTGACTGATCTTTTTGAGAATCATCGCTTCACCGATCATTTACTCGCTCTCAGCAATCACGATCGTGATCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCAAACGACTTGGGCATTTCGGGGTTTGAAGATAGCGGTGACATTGAGTCTAACTATACTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGATTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAACAGTGACCTTCGGGTTGTTGATGTAGATTCGGACTCGGATTATGATAATGGAATTATCGGTGCTCTGCAATTTGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCAGATAACTTCAGCTTTTGTTTTCAGCAGAGGAATTCGTACGAGGATTTTGAATGGGAGGAGGTTGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTGGTGGAGGGAGCCGAGGAATTGACCCTTGCATCTGGTTTTTCCAATGATGAAGAATCAGGAGAAGAGGCGGGCCGATGGGAAATTTTATTGGTAATGGACGACATTGGGAGCAATACGAATCGGGAACGGGATAATGATGCTGAGGCGTACGTAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAACGCTTTGAAGGGTTGTCCTCCAGCAGCTAAGAGTGCTATGGAGAATCTTCCTCTGGTTGAGTTAAAAACAGAGAATATACTGACAGAGGAAGTGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCTTGCTGCCACTATTACCATGATGACTGCATTGTGCAATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCCACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAGAGGGCCAGTGGTGGCCAGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAA

Coding sequence (CDS)

ATGGCTTTTTTCTCTATTCCTAAACTTCATCTTCAACCTAACATGCCTCTTCCTGATCAACCCCGCTCCGATTCTCCTTCTCCGGTGCCGTCGATCACATCCTTCCGCCGCGACGAATTCCCCAACCCCATGGATTTCAACCCTTTTGCCACCGTCCCCTCCATCGTCGCTCCTCCCGACGATTCCCTCCGTTTTGAGACCGATTGGTTCTCCGACACGGATTCCGACTCCGAACGCGATGTCAGTTGCTTGGTGACTGATCTTTTTGAGAATCATCGCTTCACCGATCATTTACTCGCTCTCAGCAATCACGATCGTGATCTGGATTCTGGGCCGGACCCATTTACGCATTCTGCAAACGACTTGGGCATTTCGGGGTTTGAAGATAGCGGTGACATTGAGTCTAACTATACTGAGGAGCTGTGGTCGACGTTTGCTGTGGAATCTGATAGCCGGGATTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAACAGTGACCTTCGGGTTGTTGATGTAGATTCGGACTCGGATTATGATAATGGAATTATCGGTGCTCTGCAATTTGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTTGAAAGTCCGAATCCTCTCTCAGATAACTTCAGCTTTTGTTTTCAGCAGAGGAATTCGTACGAGGATTTTGAATGGGAGGAGGTTGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTGGTGGAGGGAGCCGAGGAATTGACCCTTGCATCTGGTTTTTCCAATGATGAAGAATCAGGAGAAGAGGCGGGCCGATGGGAAATTTTATTGGTAATGGACGACATTGGGAGCAATACGAATCGGGAACGGGATAATGATGCTGAGGCGTACGTAGCCGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAACGCTTTGAAGGGTTGTCCTCCAGCAGCTAAGAGTGCTATGGAGAATCTTCCTCTGGTTGAGTTAAAAACAGAGAATATACTGACAGAGGAAGTGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCTTGCTGCCACTATTACCATGATGACTGCATTGTGCAATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCCACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAGAGGGCCAGTGGTGGCCAGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAA

Protein sequence

MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPDDSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSANDLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSDYDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQDDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTDLQVRYNFELIA
Homology
BLAST of Cp4.1LG01g22200 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.0e-25
Identity = 60/126 (47.62%), Postives = 78/126 (61.90%), Query Frame = 0

Query: 297 ADQDDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAV 356
           A+  DY      + L  Q  EN+    G PPA+KSA+E LPLV + T++ L  E   CAV
Sbjct: 168 ANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNI-TKSNLNSEFNQCAV 227

Query: 357 CKDKFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYER--RKSQRAS 416
           C D F    + +++PC H YH DC++ WL + N+CPVCR+ELPTDDPDYER  R +Q  S
Sbjct: 228 CMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 287

Query: 417 GGQQTD 421
           GG   D
Sbjct: 288 GGNDGD 292

BLAST of Cp4.1LG01g22200 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.9e-25
Identity = 55/119 (46.22%), Postives = 78/119 (65.55%), Query Frame = 0

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DY +    + L  Q  EN+    G PPA+KSA++ LP V++ T+++L  E+  CAVC D+
Sbjct: 164 DYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV-TKDMLKSEMNQCAVCMDE 223

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRK--SQRASGGQ 418
           F     V+++PC H +H DC++ WL + N+CPVCR+ELPTDDPDYE R   SQ +  GQ
Sbjct: 224 FEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQ 281

BLAST of Cp4.1LG01g22200 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.2e-23
Identity = 81/226 (35.84%), Postives = 110/226 (48.67%), Query Frame = 0

Query: 212 DNFSFCFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELTLASGFSNDEESGEEAGRW 271
           D F      RN  +D E E+ ++  +E E      +  E LT+     NDEE  E+  R 
Sbjct: 105 DFFRLELNSRNEIDDDEDEDEDDGDEEEE------DEEENLTV-----NDEEDEEDDLRR 164

Query: 272 EILLVMDDIGSNTNRER------------DN------DAEAYVADQDDYM-YAAEYDTLF 331
                +    S T R R            DN      +++ Y  +  DY+  AA Y+ L 
Sbjct: 165 RNRFPLTTTQSRTGRNRILDWAEILMGIEDNSIEFRMESDRYAGNPADYIDDAAGYEALL 224

Query: 332 GQFVENE----NALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVR 391
               E +       +G PPAAKSA+E L   E+ +     E V++CAVCKD   M E  +
Sbjct: 225 QNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSE--GEMVMVCAVCKDGMVMGETGK 284

Query: 392 KLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRAS 415
           KLPC H YH DCIV WL  RN+CPVCR++L TDD +YE  + +R S
Sbjct: 285 KLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTS 317

BLAST of Cp4.1LG01g22200 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-21
Identity = 47/108 (43.52%), Postives = 71/108 (65.74%), Query Frame = 0

Query: 301 DYMYAAEYDTLFGQFVENE----NALKGC--PPAAKSAMENLPLVELKTENILTEEVVIC 360
           +++  + +D L  Q  + E      L+ C  PPA+KSA+E LPL+E+   ++L++    C
Sbjct: 142 EFLLGSGFDRLLDQISQIELNTNRNLRSCEHPPASKSAIEALPLIEIDPTHLLSDSQSHC 201

Query: 361 AVCKDKFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDD 403
           AVCK+ F ++   R++PC H YH DCI+ WL IRN+CPVCR+ELP +D
Sbjct: 202 AVCKENFVLKSSAREMPCNHIYHPDCILPWLAIRNSCPVCRHELPAED 249

BLAST of Cp4.1LG01g22200 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-18
Identity = 43/78 (55.13%), Postives = 59/78 (75.64%), Query Frame = 0

Query: 324 GCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCHYYHDDCIVQ 383
           G PPA+KSA+E+LP VE+   +I +E    CAVC + F  E + R++PC H +HDDCIV 
Sbjct: 171 GNPPASKSAIESLPRVEISDCHIGSE--ANCAVCTEIFETETEAREMPCKHLFHDDCIVP 230

Query: 384 WLNIRNTCPVCRYELPTD 402
           WL+IRN+CPVCR+ELP++
Sbjct: 231 WLSIRNSCPVCRFELPSE 246

BLAST of Cp4.1LG01g22200 vs. NCBI nr
Match: XP_023526783.1 (uncharacterized protein LOC111790174 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. NCBI nr
Match: XP_022921923.1 (uncharacterized protein LOC111430034 [Cucurbita moschata])

HSP 1 Score: 856 bits (2212), Expect = 2.57e-313
Identity = 424/431 (98.38%), Postives = 427/431 (99.07%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHD DLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVESDSR SEVDVSMQVADSINSDLRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           Y+NGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELT+ASGFSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. NCBI nr
Match: KAG7033230.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852 bits (2202), Expect = 8.58e-312
Identity = 423/431 (98.14%), Postives = 425/431 (98.61%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDE PNPMDFNPFATVPSIVAPPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEVPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSER VSCLVTDLFENHRFTDHLLALSNHD DLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERHVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVESDSR SEVDVSMQVADSINSDLRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELT+ASGFSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. NCBI nr
Match: XP_022989863.1 (uncharacterized protein LOC111486927 [Cucurbita maxima])

HSP 1 Score: 845 bits (2184), Expect = 4.76e-309
Identity = 419/431 (97.22%), Postives = 421/431 (97.68%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIV PPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVTPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLL LSNHD DLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLTLSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVES SR SEVDVSMQVADSINSDLRVVDVDSD+D
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESGSRVSEVDVSMQVADSINSDLRVVDVDSDTD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELTLAS FSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTLASDFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYD LFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDALFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQR SGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRTSGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. NCBI nr
Match: KAG6602550.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 813 bits (2100), Expect = 7.19e-296
Identity = 403/411 (98.05%), Postives = 406/411 (98.78%), Query Frame = 0

Query: 15  MPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPDDSLRFETDWFSDTD 74
           MPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPDDSLRFETDWFSDTD
Sbjct: 1   MPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPDDSLRFETDWFSDTD 60

Query: 75  SDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSANDLGISGFEDSGDIE 134
           SDSER VSCLVTDLFENHRFTDHLLALSNHD DLDSGPDPFTHSANDLGISGFEDSGDIE
Sbjct: 61  SDSERHVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSANDLGISGFEDSGDIE 120

Query: 135 SNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSDYDNGIIGALQFVTG 194
           SNYTEELWSTFAVESDSR SEVDVSMQVADSINSDLRVVDVDSDSDYDNGIIGALQFVTG
Sbjct: 121 SNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSDYDNGIIGALQFVTG 180

Query: 195 DDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELTL 254
           DDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELT+
Sbjct: 181 DDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELTI 240

Query: 255 ASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQDDYMYAAEYDTLFGQ 314
           ASGFSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQDDYMYAAEYDTLFGQ
Sbjct: 241 ASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQDDYMYAAEYDTLFGQ 300

Query: 315 FVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCH 374
           FVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCH
Sbjct: 301 FVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCH 360

Query: 375 YYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTDLQVRY 425
           YYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTDLQVR+
Sbjct: 361 YYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTDLQVRF 411

BLAST of Cp4.1LG01g22200 vs. ExPASy TrEMBL
Match: A0A6J1E557 (uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC111430034 PE=4 SV=1)

HSP 1 Score: 856 bits (2212), Expect = 1.24e-313
Identity = 424/431 (98.38%), Postives = 427/431 (99.07%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHD DLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVESDSR SEVDVSMQVADSINSDLRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           Y+NGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELT+ASGFSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. ExPASy TrEMBL
Match: A0A6J1JRJ8 (uncharacterized protein LOC111486927 OS=Cucurbita maxima OX=3661 GN=LOC111486927 PE=4 SV=1)

HSP 1 Score: 845 bits (2184), Expect = 2.30e-309
Identity = 419/431 (97.22%), Postives = 421/431 (97.68%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIV PPD
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVTPPD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLL LSNHD DLDSGPDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLTLSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFEDSGDIESNYTEELWSTFAVES SR SEVDVSMQVADSINSDLRVVDVDSD+D
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESGSRVSEVDVSMQVADSINSDLRVVDVDSDTD 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240
           YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300
           ISSVVVEGAEELTLAS FSNDEESGEEAGRWEILLVMDDIGSNTN ERDNDAEAYVADQD
Sbjct: 241 ISSVVVEGAEELTLASDFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360
           DYMYAAEYD LFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK
Sbjct: 301 DYMYAAEYDALFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQR SGGQQTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRTSGGQQTD 420

Query: 421 LQVRYNFELIA 431
           LQVRYNFELIA
Sbjct: 421 LQVRYNFELIA 431

BLAST of Cp4.1LG01g22200 vs. ExPASy TrEMBL
Match: A0A6J1JWL4 (uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 5.05e-256
Identity = 354/432 (81.94%), Postives = 385/432 (89.12%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           M F SIPKL+LQPN+ LPDQ  SDSPSPVPS+ S RRD+ PN MDFNPFAT+ SIV   D
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DS  F+TDWFSD DS+SERDVSC VTDLFEN RFTDHL A S++DR LDSGPDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFED+G+IES+Y EELWS FA+ESDSR SEVDVSM VADS  S LR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRNSYEDFEWEEVEERVDER 240
            DNG+IGALQ VTGDDGEND NWVE+PNPLSD+FSFCFQ QR  YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQ 300
           +  S VV+GAEEL+LASGFSN+EESGEEAGRWEILLVM++I  NTN ERDNDAEAY+ADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKG PPAAKS++ENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQT 420
           KFSMEEKVRKLPC HYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRA GG QT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVRYNFELIA 431
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Cp4.1LG01g22200 vs. ExPASy TrEMBL
Match: A0A6J1FE59 (uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444905 PE=4 SV=1)

HSP 1 Score: 710 bits (1832), Expect = 1.02e-255
Identity = 353/432 (81.71%), Postives = 385/432 (89.12%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           M F SIPKL+LQPN+ LPDQ  SDSPSPVPS+ S RRD+ PN MDFNPFAT+ SIV   D
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DS  F+TDWFSD DS+SERDVSC VTDLFEN RFTDHL A S++DR LDSGPDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFED+G+IES+Y EELWS FA+ESDSR SEVDVSM VADS  S LR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRNSYEDFEWEEVEERVDER 240
            DNG+IGALQ VTGDDGEND NWVE+PNPLSD+FSFCFQ QR  YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQ 300
           +  S VV+GAEEL+LASGFSN+EE+GEEAGRWEILLVM+DI  NTN ERDNDAEAY+ADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEEAGEEAGRWEILLVMNDIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKG PPAAKS++ENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQT 420
           KFSMEEKVRKLPC HYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKS+RA GG QT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSRRAGGGLQT 420

Query: 421 DLQVRYNFELIA 431
           DL+VRYNFELIA
Sbjct: 421 DLRVRYNFELIA 432

BLAST of Cp4.1LG01g22200 vs. ExPASy TrEMBL
Match: A0A6J1JYD4 (uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 699 bits (1804), Expect = 1.94e-251
Identity = 348/428 (81.31%), Postives = 380/428 (88.79%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60
           M F SIPKL+LQPN+ LPDQ  SDSPSPVPS+ S RRD+ PN MDFNPFAT+ SIV   D
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120
           DS  F+TDWFSD DS+SERDVSC VTDLFEN RFTDHL A S++DR LDSGPDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180
           DLGISGFED+G+IES+Y EELWS FA+ESDSR SEVDVSM VADS  S LR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRNSYEDFEWEEVEERVDER 240
            DNG+IGALQ VTGDDGEND NWVE+PNPLSD+FSFCFQ QR  YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQ 300
           +  S VV+GAEEL+LASGFSN+EESGEEAGRWEILLVM++I  NTN ERDNDAEAY+ADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKG PPAAKS++ENLPLVELKTEN+LTEE+V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQT 420
           KFSMEEKVRKLPC HYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRA GG QT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVRYNF 427
           DL+VR+ F
Sbjct: 421 DLRVRFPF 428

BLAST of Cp4.1LG01g22200 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.4e-43
Identity = 112/327 (34.25%), Postives = 174/327 (53.21%), Query Frame = 0

Query: 109 DSGPDP-------FTHSANDLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDV--- 168
           DS PDP       F   ++D+ ++ + +SG + +     +W  +  + D  + E+ +   
Sbjct: 69  DSEPDPNSCPIDFFDRDSSDVDVAEYLESGGLTAG-DYNIWGFYDPKEDEEEEEIVLGTS 128

Query: 169 --SMQVADSINSDLRVVDVDSDSDYDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSF 228
              +Q  DS    LRV  +DSDSD ++G+   +   +  +  ND   VE    L   +  
Sbjct: 129 GSDLQPGDSGEQGLRVTGIDSDSDCEDGVFDFISEDSSGNRGNDSGRVEVGTGLPPVWDH 188

Query: 229 CFQQRNSYEDFEWEEVEERVDEREISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLV 288
            F +     D EWEEV+  ++    S    E  +E    S  S D+E  +    W++LL 
Sbjct: 189 LFGEGTVLADEEWEEVQNAINWTAFSG--PEDEDEEDELSSLSRDDEEEDHELDWQVLLT 248

Query: 289 MDDIGSNTNRER-------DNDAEAYV----ADQDDYMYAAEY--DTLFGQFVENENALK 348
           ++++ +   +         D D + Y+     D+ D  ++  Y  D + GQ  ++E  ++
Sbjct: 249 VNNVVNYIEQAEGIMLNPDDIDPDYYLYLSGLDEFDENHSGHYDADAILGQMFDDETGIR 308

Query: 349 GCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCHYYHDDCIVQ 408
           G PPAAKS +++LP+VEL  E  L +   +CAVCKD+  +EEKVR+LPC H+YH +CI+ 
Sbjct: 309 GNPPAAKSVIQDLPVVELAVEE-LDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECIIP 368

Query: 409 WLNIRNTCPVCRYELPTDDPDYERRKS 411
           WL IRNTCPVCRYELPTDD +YER KS
Sbjct: 369 WLGIRNTCPVCRYELPTDDLEYERHKS 391

BLAST of Cp4.1LG01g22200 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 143.3 bits (360), Expect = 4.6e-34
Identity = 129/392 (32.91%), Postives = 187/392 (47.70%), Query Frame = 0

Query: 54  SIVAPPDDSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPD 113
           S V PP D      D F +  +D  R  +C V             +   N D  ++ G +
Sbjct: 12  SQVTPPHDDSNIHGD-FDNEATDESRSETCSV-------------VIEGNCDMVIELGLE 71

Query: 114 PFTHSANDLGISGFEDSGDIESNYTEELWSTFAVESDSRD---SEVDVSMQVAD--SINS 173
             +    +LG S  + S D +             E D  D      D  ++  D  S  +
Sbjct: 72  SGSVIVENLGDSDIDVSNDFD-------------EPDDDDFFVGRTDFGLEFRDIASSGN 131

Query: 174 DLRVVDVDSDSDYDNGI----------IGALQFVTGDDGENDVNWVESPNPLSDNFSFCF 233
           D+R++ V+S SD D+G+          +        DD E + + V    PL  +     
Sbjct: 132 DIRLITVESGSDDDDGVENERELWGIDLNEEDVYVNDDDEYEDDDVSVTIPLCWDSLQLE 191

Query: 234 QQRNSYEDFEWEEVEER-----VDEREISSVVVE---GAEELTLASGFSNDE-----ESG 293
            +  + ++F+WEEV         DEREI S   +     E L  AS   + E     E  
Sbjct: 192 DREVTADEFDWEEVGGGGGGGVDDEREIRSGFAQIDMNDESLISASPIISLEGLVTRERA 251

Query: 294 EEAGR--WEILLVMDDIGSNTNRERDNDAEAYV-ADQDDYMYAAEYDTLFGQFVENENAL 353
           E +G   WE+LL   +     N + DN  E Y+  D DDY+   +YD LF QF + E ++
Sbjct: 252 EGSGNLGWEVLL---NHTLEINFDVDN-RELYIGGDHDDYV--QDYDMLFEQFADAEVSV 311

Query: 354 KGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDKFSMEEKVRKLPCCHYYHDDCIV 413
            G PP +KS + NLP+V L+ EN   +  ++CAVCKD+ ++  K  +LPC H YH +CIV
Sbjct: 312 IGLPPTSKSFLNNLPVVLLEGEND-DDGGLVCAVCKDEMNIGNKAVQLPCNHKYHSECIV 369

Query: 414 QWLNIRNTCPVCRYELPTDDPDYERRKSQRAS 415
            WL +RNTCPVCRYELPTDD +YE+RK+QR +
Sbjct: 372 PWLKVRNTCPVCRYELPTDDAEYEQRKTQRTT 369

BLAST of Cp4.1LG01g22200 vs. TAIR 10
Match: AT5G15820.1 (RING/U-box superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 5.3e-30
Identity = 106/295 (35.93%), Postives = 145/295 (49.15%), Query Frame = 0

Query: 129 DSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDV----DSDSDYDNG 188
           DSG  +S Y E  +  F    D    EVD   +V  S N  +         D D D   G
Sbjct: 68  DSGS-DSMYAEPEFIDF---FDRESYEVDTVREVCVSSNQRVSTPGYFNIWDQDVDLGLG 127

Query: 189 I-IGALQ---FVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVD-ER 248
           I +G+      + GD G   V       P+  N  F  +     E  EWE     +   +
Sbjct: 128 IGLGSRSGSGQLPGDSGGVGVEVGRGVTPVEYNL-FGEEAMVVDEVLEWENFNNAIHLVQ 187

Query: 249 EISSVVVEGAEELTLASGFSNDEESGEEAG-----RWEILLVMDDIGSNTNRERDNDAEA 308
           E +   +EG EE         DE   E A       WEILL  +      +   D D E 
Sbjct: 188 EPAYASMEGEEE------EEEDEVVMEFAASIYSDAWEILLYDN---MTNSAPMDLDVEV 247

Query: 309 YVADQDDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVIC 368
           ++   D Y    +Y+ + GQ  +NE  +KG PPA+KS ++ LP VEL  E  L+   ++C
Sbjct: 248 WLDSVDGYA-PMDYNAIIGQMFDNETGIKGTPPASKSVVDGLPDVELTIEE-LSSVSIVC 307

Query: 369 AVCKDKFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRK 410
           A+CKD+   +EKV++LPC HYYH +CI+ WL IRNTCPVCR+ELPTDD +YER++
Sbjct: 308 AICKDEVVFKEKVKRLPCKHYYHGECIIPWLGIRNTCPVCRHELPTDDLEYERKR 346

BLAST of Cp4.1LG01g22200 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 2.7e-26
Identity = 84/258 (32.56%), Postives = 135/258 (52.33%), Query Frame = 0

Query: 168 SDLRVVDVDSDSDYDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRN----- 227
           S++  +D++ + +Y N           DD +ND    +     S     C+         
Sbjct: 182 SEIWGIDLNEEDEYVN-----------DDDDND----DEEEDASVTIPLCWDSLQLEDLG 241

Query: 228 -SYEDFEWEEVEERVDEREISSVVVEGAE----ELTLASGFSND-----EESGEEAGRWE 287
            + ED EWEEV+   DERE+ SV+ E  +     +++A+  S +     E  G     WE
Sbjct: 242 INNEDCEWEEVDSD-DEREVLSVLAEADDNNSVSVSVAATISLEDLAISERRGSSNLGWE 301

Query: 288 ILLVMDDIGSNTNRERDNDAEAYVADQD-------DYMYAAEYDTLFGQFVENENALKGC 347
           +LL    +  N + + +++ E Y+ D D       DY++  EY+ LF   + +     G 
Sbjct: 302 VLLNSRSLEFNLD-DAESNLELYIGDIDHEEEDYEDYLHTTEYEMLFEAEISSG---IGK 361

Query: 348 PPAAKSAMENLPLVELKTENIL--TEEVVICAVCKDKFSMEEKVRKLPCCHYYHDDCIVQ 402
           PPA+KS ++NL +  L  E+++   ++ V CAVCK++  + ++V +LPC H YH +CIV 
Sbjct: 362 PPASKSFIKNLKVSPLSNEDVMENDDDAVCCAVCKEEMIVGKEVAELPCRHKYHSECIVP 419

BLAST of Cp4.1LG01g22200 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 4.6e-26
Identity = 93/299 (31.10%), Postives = 147/299 (49.16%), Query Frame = 0

Query: 115 FTHSANDLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVD 174
           F+ S +D     F++ G+ ESN +  L+     ++D+    +  S    +S  S  R  D
Sbjct: 139 FSESESD----DFDNFGETESNASFSLYRFPHADNDT----ISFSAYGGESDASTDRHAD 198

Query: 175 VDSDSDYDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEE--V 234
           +               FV  DD  +D+++    +P+    +   Q  +  ED EWEE   
Sbjct: 199 I---------------FVQPDD-RSDIDFDTDIDPMRAGLN---QWNSDEEDREWEEGAG 258

Query: 235 EERVDEREISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSN--TNRERDN 294
              V      + +   +E  +  + F + E   E + R  I+     +  N  T  E D 
Sbjct: 259 PSGVAGTRYRNYLASPSESYSSMTRFDSPEL--ERSFRQRIIERRHSLSRNIFTGLE-DL 318

Query: 295 DAEAYVADQDDYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEE 354
           D   Y A+  DY+    +D L  Q  E++N+ +G PPA+ S + NLP V +  E+++  +
Sbjct: 319 DFSPYAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHVM--K 378

Query: 355 VVICAVCKDKFSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRK 410
            ++CA+CK+ FS+  +  +LPC H YH  CIV WL+ RN+CP+CRYELPTDD DYE  K
Sbjct: 379 GLVCAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEEGK 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0CH301.0e-2547.62E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN72.9e-2546.22E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9SPL21.2e-2335.84E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
O222834.4e-2143.52Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T51.2e-1855.13E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023526783.10.0100.00uncharacterized protein LOC111790174 [Cucurbita pepo subsp. pepo][more]
XP_022921923.12.57e-31398.38uncharacterized protein LOC111430034 [Cucurbita moschata][more]
KAG7033230.18.58e-31298.14E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022989863.14.76e-30997.22uncharacterized protein LOC111486927 [Cucurbita maxima][more]
KAG6602550.17.19e-29698.05E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1E5571.24e-31398.38uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC1114300... [more]
A0A6J1JRJ82.30e-30997.22uncharacterized protein LOC111486927 OS=Cucurbita maxima OX=3661 GN=LOC111486927... [more]
A0A6J1JWL45.05e-25681.94uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FE591.02e-25581.71uncharacterized protein LOC111444905 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JYD41.94e-25181.31uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G02340.12.4e-4334.25RING/U-box superfamily protein [more]
AT5G08139.14.6e-3432.91RING/U-box superfamily protein [more]
AT5G15820.15.3e-3035.93RING/U-box superfamily protein [more]
AT5G60820.12.7e-2632.56RING/U-box superfamily protein [more]
AT5G01980.14.6e-2631.10RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 354..394
e-value: 1.3E-6
score: 38.0
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 354..395
e-value: 5.9E-11
score: 42.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 354..395
score: 12.326995
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 314..401
e-value: 6.1E-20
score: 72.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..42
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 115..415
NoneNo IPR availablePANTHERPTHR15710:SF108E3 UBIQUITIN-PROTEIN LIGASE CIP8-LIKE ISOFORM X1coord: 115..415
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 354..395
e-value: 1.82574E-21
score: 84.6768
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 335..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g22200.1Cp4.1LG01g22200.1mRNA