Cp4.1LG01g22130 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g22130 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.3e-38
Identity = 104/330 (31.52%), Postives = 168/330 (50.91%), Query Frame = 0

Query: 100 SDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGIC 159
           S G++  +SY     G  +F++ ++P     K  V   HGYG   ++ F+        IC
Sbjct: 34  SQGVRNSKSYFETPNG-KLFTQSFLPLDGEIKGTVYMSHGYGSDTSWMFQ-------KIC 93

Query: 160 ISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALP 219
           +S      +  GY VF+ D  G G S+G+  ++   +K+    +  +  V+ +  +  LP
Sbjct: 94  MS-----FSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLP 153

Query: 220 SFLFGQSLGGAVALKVHLK-QPHSWTGAVLVAPMCKIADDMVPPWA---LAQILIGVAKF 279
           +FLFG+S+GG V L ++ + +P +WTG +  AP+  I +DM P  A      +L G+A  
Sbjct: 154 AFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLA-- 213

Query: 280 LPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELE 339
              +  +P   +   A +D +  ++ A N   Y  KPR+ T  E+LR TQ ++    +  
Sbjct: 214 -DTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQENFGK-- 273

Query: 340 NLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKD 399
                               V++P+   HG AD VT P+ SK LYEKASS+DK++K+Y+ 
Sbjct: 274 --------------------VTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEG 325

Query: 400 AYHSLLEGEPDEIIIQVFDDIISWLNEHCK 426
            YHSL++GEPDE    V  D+  W++E  K
Sbjct: 334 MYHSLIQGEPDENAEIVLKDMREWIDEKVK 325

BLAST of Cp4.1LG01g22130 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 1.3e-20
Identity = 87/332 (26.20%), Postives = 150/332 (45.18%), Query Frame = 0

Query: 97  KTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFC 156
           +TP      +  + VN+ G  +F + W P T  PKA++   HG G+  +  +E + R+  
Sbjct: 9   RTPQSIPYQDLPHLVNADGQYLFCRYWKP-TGTPKALIFVSHGAGEH-SGRYEELARMLM 68

Query: 157 GICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFS 216
           G+ +             VF+ D+ G G SEG    +  F   V DV++H   +++  ++ 
Sbjct: 69  GLDLL------------VFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQK--DYP 128

Query: 217 ALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAK-- 276
            LP FL G S+GGA+A+    ++P  + G VL++P+      +  P +     +  AK  
Sbjct: 129 GLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLV-----LANPESATTFKVLAAKVL 188

Query: 277 --FLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQT--AVEMLRTTQEIERR 336
              LP   L P     +++       E+  YN      +  L+    +++L     +ER 
Sbjct: 189 NLVLPNLSLGP----IDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERA 248

Query: 337 LKELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSI 396
           L +L                      ++P L+L G AD + D   +  L E A S DK++
Sbjct: 249 LPKL----------------------TVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTL 292

Query: 397 KLYKDAYHSLLEGEPDEIIIQVFDDIISWLNE 423
           K+Y+ AYH +L  E  E+   VF +I  W+++
Sbjct: 309 KIYEGAYH-VLHKELPEVTNSVFHEINMWVSQ 292

BLAST of Cp4.1LG01g22130 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.6e-20
Identity = 83/329 (25.23%), Postives = 150/329 (45.59%), Query Frame = 0

Query: 97  KTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFC 156
           +TP +    +  + VN+ G  +F + W P    PKA++   HG G+ C  + E       
Sbjct: 9   RTPQNVPYQDLPHLVNADGQYLFCRYWKPSGT-PKALIFVSHGAGEHCGRYDE------- 68

Query: 157 GICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFS 216
                 +A  L      VF+ D+ G G SEG    +  F   V DV++H   +++  ++ 
Sbjct: 69  ------LAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQK--DYP 128

Query: 217 ALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFL 276
            +P FL G S+GGA+++ V  ++P  ++G VL++P+      +  P + + + +  AK L
Sbjct: 129 DVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLV-----LANPESASTLKVLAAKLL 188

Query: 277 PKYKLVPQKNLA--EAAFRDMKHRELTAYNVIAYQHKPRLQT--AVEMLRTTQEIERRLK 336
               ++P   L   +++       E+  YN      +  L+    +++L     +ER + 
Sbjct: 189 --NFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMP 248

Query: 337 ELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKL 396
            L                      +LP L+L G AD + D   +  L E + S DK++K+
Sbjct: 249 RL----------------------TLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKM 291

Query: 397 YKDAYHSLLEGEPDEIIIQVFDDIISWLN 422
           Y+ AYH +L  E  E+   V  ++ SW++
Sbjct: 309 YEGAYH-VLHRELPEVTNSVLHEVNSWVS 291

BLAST of Cp4.1LG01g22130 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 8.1e-20
Identity = 80/329 (24.32%), Postives = 152/329 (46.20%), Query Frame = 0

Query: 97  KTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFC 156
           +TP +    +  + VN+ G  +F + W P    PKA++   HG G+ C  + E       
Sbjct: 9   RTPQNVPYQDLPHLVNADGQYLFCRYWKPSGT-PKALIFVSHGAGEHCGRYDE------- 68

Query: 157 GICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFS 216
                 +A+ L      VF+ D+ G G SEG    +  F   V D+++H + V++  ++ 
Sbjct: 69  ------LAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQK--DYP 128

Query: 217 ALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFL 276
            +P FL G S+GGA+++    ++P  ++G +L++P+      +  P + + + +  AK L
Sbjct: 129 EVPVFLLGHSMGGAISILAAAERPTHFSGMILISPLI-----LANPESASTLKVLAAKLL 188

Query: 277 PKYKLVPQKNLA--EAAFRDMKHRELTAYN--VIAYQHKPRLQTAVEMLRTTQEIERRLK 336
               ++P  +L   +++       E+  YN   +      ++   +++L     +ER + 
Sbjct: 189 --NFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMP 248

Query: 337 ELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKL 396
            L                      +LP L+L G AD + D   +  L E + S DK++K+
Sbjct: 249 RL----------------------TLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKM 291

Query: 397 YKDAYHSLLEGEPDEIIIQVFDDIISWLN 422
           Y+ AYH +L  E  E+   V  +I +W++
Sbjct: 309 YEGAYH-VLHKELPEVTNSVLHEINTWVS 291

BLAST of Cp4.1LG01g22130 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.0e-15
Identity = 77/313 (24.60%), Postives = 133/313 (42.49%), Query Frame = 0

Query: 115 GLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGV 174
           G+ I    W P+T  P+ +V   HGY +       G Y          +A++   +G  V
Sbjct: 16  GVRIVYDVWTPDT-DPRGVVVLAHGYAE-----HAGRYH--------HVAQRFGAAGLLV 75

Query: 175 FSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALK 234
           +++D+ G G S G    +    + V+D       +  N +   LP  + G S+GG +   
Sbjct: 76  YALDHRGHGRSGGKRVHLRDLSEFVED-FRTLVGIAAN-DHPTLPRIVLGHSMGGGIVFA 135

Query: 235 VHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKL-VPQKNL-AEAAF 294
              + P  ++  VL  P     D + P      +L+ VAK L K    +P +NL A+A  
Sbjct: 136 YGARYPGEYSAMVLSGPAVNAHDGVSP------VLVAVAKVLGKLAPGIPVENLDADAVS 195

Query: 295 RDMKHRELTAYNVIAYQHKPRLQTAV--EMLRTTQEIERRLKELENLFVTPWLLDIPFNP 354
           RD +   + AY      H  +L   +   ++   Q + +R   L                
Sbjct: 196 RDPE--VVAAYKADPMVHHGKLPAGIARALIGLGQSMPQRAAAL---------------- 255

Query: 355 LFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIII 414
                 + PLL++HG+ D +   + S+ L ++ +S D  +K+Y   YH +   EP++ + 
Sbjct: 256 ------TAPLLVVHGDKDRLIPVAGSRLLVDRVASEDVHLKVYPGLYHEVF-NEPEQKL- 279

Query: 415 QVFDDIISWLNEH 424
            V DD+ SW+  H
Sbjct: 316 -VLDDVTSWIVSH 279

BLAST of Cp4.1LG01g22130 vs. NCBI nr
Match: XP_023532059.1 (caffeoylshikimate esterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1973), Expect = 1.18e-277
Identity = 391/425 (92.00%), Postives = 391/425 (92.00%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS
Sbjct: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 391

Query: 421 NEHCK 425
           NEHCK
Sbjct: 421 NEHCK 391

BLAST of Cp4.1LG01g22130 vs. NCBI nr
Match: XP_022990122.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 757 bits (1955), Expect = 6.53e-275
Identity = 387/425 (91.06%), Postives = 389/425 (91.53%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           M VRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKG SRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MTVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGYSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           D+LLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS
Sbjct: 61  DKLLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAY+HKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYKHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 391

Query: 421 NEHCK 425
           NEHCK
Sbjct: 421 NEHCK 391

BLAST of Cp4.1LG01g22130 vs. NCBI nr
Match: XP_022954732.1 (caffeoylshikimate esterase [Cucurbita moschata])

HSP 1 Score: 754 bits (1948), Expect = 7.33e-274
Identity = 386/424 (91.04%), Postives = 388/424 (91.51%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKG SRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGYSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLK EESYEVNSRGLSIFS
Sbjct: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKTEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           H+WTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HAWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAY+HKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYKHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 390

Query: 421 NEHC 424
           NEHC
Sbjct: 421 NEHC 390

BLAST of Cp4.1LG01g22130 vs. NCBI nr
Match: KAG6602575.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 753 bits (1943), Expect = 1.41e-272
Identity = 386/425 (90.82%), Postives = 388/425 (91.29%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKG SRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGYSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLK EESYEVNSRGLSIFS
Sbjct: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKTEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           H+WTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HAWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAY+HKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYKHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 391

Query: 421 NEHCK 425
           NEH K
Sbjct: 421 NEHWK 391

BLAST of Cp4.1LG01g22130 vs. NCBI nr
Match: XP_038890284.1 (caffeoylshikimate esterase isoform X1 [Benincasa hispida])

HSP 1 Score: 639 bits (1647), Expect = 6.92e-228
Identity = 324/403 (80.40%), Postives = 346/403 (85.86%), Query Frame = 0

Query: 23  ARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVNDELLKLLDANMDDVSTRRRVRE 82
           A  IANTRSQ   KG  RGK S R A+MAP+ KL GV+DEL KLLDANMD V+TRRR RE
Sbjct: 30  ATTIANTRSQCIDKGGLRGKRSFRNAIMAPRKKLQGVDDELQKLLDANMDGVATRRRARE 89

Query: 83  AFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGD 142
           AFK IQLG+DHILFKTPSDGLKMEE+YEVNSRGLSIFSK WIPETVRPKAMVCYCHGYGD
Sbjct: 90  AFKNIQLGVDHILFKTPSDGLKMEETYEVNSRGLSIFSKSWIPETVRPKAMVCYCHGYGD 149

Query: 143 TCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDV 202
           TCTFFFEGI            ARKLALSGYGVFSMDYPGFGLSEGLHGFIP+FD+IVDDV
Sbjct: 150 TCTFFFEGI------------ARKLALSGYGVFSMDYPGFGLSEGLHGFIPNFDRIVDDV 209

Query: 203 IEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPP 262
           IEHYSKVKENP +SALPSFLFGQSLGGAVALKVHLKQP SW+GAVLVAPMCKIADDMVPP
Sbjct: 210 IEHYSKVKENPAYSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPP 269

Query: 263 WALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLR 322
           WALAQ+LIGV+KFLPKYKLVPQK+LAEAAFRD+K+RELTAYNVIAY+HKPRLQTAVEML+
Sbjct: 270 WALAQVLIGVSKFLPKYKLVPQKDLAEAAFRDLKYRELTAYNVIAYKHKPRLQTAVEMLK 329

Query: 323 TTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEK 382
           TTQEIERRLKE                      +SLPLLILHGEAD VTDPSVSK LYEK
Sbjct: 330 TTQEIERRLKE----------------------ISLPLLILHGEADIVTDPSVSKLLYEK 389

Query: 383 ASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWLNEHCK 425
           ASSSDK IKLYKDAYHSLLEGEPDE+I++VFDDIISWL+E CK
Sbjct: 390 ASSSDKKIKLYKDAYHSLLEGEPDEVILEVFDDIISWLDERCK 398

BLAST of Cp4.1LG01g22130 vs. ExPASy TrEMBL
Match: A0A6J1JHR7 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111487110 PE=4 SV=1)

HSP 1 Score: 757 bits (1955), Expect = 3.16e-275
Identity = 387/425 (91.06%), Postives = 389/425 (91.53%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           M VRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKG SRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MTVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGYSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           D+LLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS
Sbjct: 61  DKLLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAY+HKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYKHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 391

Query: 421 NEHCK 425
           NEHCK
Sbjct: 421 NEHCK 391

BLAST of Cp4.1LG01g22130 vs. ExPASy TrEMBL
Match: A0A6J1GRR0 (caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111456899 PE=4 SV=1)

HSP 1 Score: 754 bits (1948), Expect = 3.55e-274
Identity = 386/424 (91.04%), Postives = 388/424 (91.51%), Query Frame = 0

Query: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVN 60
           MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKG SRGKSSSRKAVMAPKMKLHGVN
Sbjct: 1   MAVRPSPSSLEHFTFHTRPTITARAIANTRSQYSYKGYSRGKSSSRKAVMAPKMKLHGVN 60

Query: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFS 120
           DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLK EESYEVNSRGLSIFS
Sbjct: 61  DELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKTEESYEVNSRGLSIFS 120

Query: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYP 180
           KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI            ARKLALSGYGVFSMDYP
Sbjct: 121 KCWIPETVRPKAMVCYCHGYGDTCTFFFEGI------------ARKLALSGYGVFSMDYP 180

Query: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240
           GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP
Sbjct: 181 GFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQP 240

Query: 241 HSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300
           H+WTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL
Sbjct: 241 HAWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHREL 300

Query: 301 TAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPL 360
           TAYNVIAY+HKPRLQTAVEMLRTTQEIERRLKE                      VSLPL
Sbjct: 301 TAYNVIAYKHKPRLQTAVEMLRTTQEIERRLKE----------------------VSLPL 360

Query: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 420
           LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL
Sbjct: 361 LILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWL 390

Query: 421 NEHC 424
           NEHC
Sbjct: 421 NEHC 390

BLAST of Cp4.1LG01g22130 vs. ExPASy TrEMBL
Match: A0A1S3CBH4 (caffeoylshikimate esterase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498599 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 8.58e-222
Identity = 316/407 (77.64%), Postives = 342/407 (84.03%), Query Frame = 0

Query: 19  PTITARAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVNDELLKLLDANMDDVSTRR 78
           P I    ++N RS+Y  KG  RGK S RK +MAP+ KL GV+DEL KLLDANMD V+ RR
Sbjct: 27  PKIARTPVSNPRSEYIDKGGLRGKWSLRKVIMAPRKKLQGVDDELQKLLDANMDGVAARR 86

Query: 79  RVREAFKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCH 138
           R REAFK IQLGIDHILFKTPSDGLKMEE+YEVNSRGLSIFSK WIPETVRPKAMV YCH
Sbjct: 87  RAREAFKGIQLGIDHILFKTPSDGLKMEETYEVNSRGLSIFSKSWIPETVRPKAMVYYCH 146

Query: 139 GYGDTCTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKI 198
           GYGDTCTFFFEGI            ARKLA+SGYGVFSMDYPGFGLSEGLHGFIPSFD+I
Sbjct: 147 GYGDTCTFFFEGI------------ARKLAMSGYGVFSMDYPGFGLSEGLHGFIPSFDRI 206

Query: 199 VDDVIEHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADD 258
           VDDVIE YSKVKENP FSALPSFLFGQSLGGAVALKVHLKQP SW+GAVLVAPMCKIADD
Sbjct: 207 VDDVIERYSKVKENPAFSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADD 266

Query: 259 MVPPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAV 318
           MVPPWALAQ+LIG++KFLPKYKLVPQK+LAE AFRD+K+RELTAYNVIAY+ KPRLQTAV
Sbjct: 267 MVPPWALAQVLIGISKFLPKYKLVPQKDLAELAFRDLKYRELTAYNVIAYKDKPRLQTAV 326

Query: 319 EMLRTTQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKA 378
           EML+TTQEIERRLKE                      +SLPLLILHGEADTVTDPSVSK 
Sbjct: 327 EMLKTTQEIERRLKE----------------------ISLPLLILHGEADTVTDPSVSKV 386

Query: 379 LYEKASSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWLNEHCK 425
           LYEKASSSDK IKLYKDAYHSLLEGEPDE+I++VF+DI +WL+E CK
Sbjct: 387 LYEKASSSDKKIKLYKDAYHSLLEGEPDEVILEVFNDIFTWLDERCK 399

BLAST of Cp4.1LG01g22130 vs. ExPASy TrEMBL
Match: A0A0A0KSP3 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G644590 PE=4 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 1.22e-214
Identity = 301/376 (80.05%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 50  MAPKMKLHGVNDELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESY 109
           M P+ KL GV+DELLKLLDANMD V+ RRR REAFK IQLG+DHILFKTPSDG K EE+Y
Sbjct: 1   MPPRKKLQGVDDELLKLLDANMDGVAARRRAREAFKRIQLGVDHILFKTPSDGFKTEETY 60

Query: 110 EVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLAL 169
           EVNSRGLSIFSKCWIPETVRPKAMV YCHGYGDTCTFFFEGI            ARKLAL
Sbjct: 61  EVNSRGLSIFSKCWIPETVRPKAMVYYCHGYGDTCTFFFEGI------------ARKLAL 120

Query: 170 SGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGG 229
           SGYGVFSMDYPGFGLSEGLHGFIPSFD+IVDDVIE YSKVKENP FSALPSFLFGQSLGG
Sbjct: 121 SGYGVFSMDYPGFGLSEGLHGFIPSFDRIVDDVIERYSKVKENPAFSALPSFLFGQSLGG 180

Query: 230 AVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAE 289
           AV+LKVHLKQP SW+GAVLVAPMCKIADDMVPPWA+AQ+LIGV+KFLPKYKLVPQK+LAE
Sbjct: 181 AVSLKVHLKQPRSWSGAVLVAPMCKIADDMVPPWAVAQVLIGVSKFLPKYKLVPQKDLAE 240

Query: 290 AAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFN 349
            AFRD+K+RELTAYNVIAY+ KPRLQTAVEML+TTQEIERRLKE                
Sbjct: 241 VAFRDLKYRELTAYNVIAYKDKPRLQTAVEMLKTTQEIERRLKE---------------- 300

Query: 350 PLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEII 409
                 +SLPLLILHGEADTVTDPSVSK LYEKASSSDK IKLYKDAYHSLLEGEPDE+I
Sbjct: 301 ------ISLPLLILHGEADTVTDPSVSKVLYEKASSSDKKIKLYKDAYHSLLEGEPDEVI 342

Query: 410 IQVFDDIISWLNEHCK 425
           ++VF+DII+WL+E CK
Sbjct: 361 LEVFNDIITWLDERCK 342

BLAST of Cp4.1LG01g22130 vs. ExPASy TrEMBL
Match: A0A6J1BXY1 (caffeoylshikimate esterase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006719 PE=4 SV=1)

HSP 1 Score: 603 bits (1556), Expect = 1.90e-214
Identity = 300/402 (74.63%), Postives = 340/402 (84.58%), Query Frame = 0

Query: 24  RAIANTRSQYSYKGSSRGKSSSRKAVMAPKMKLHGVNDELLKLLDANMDDVSTRRRVREA 83
           R  ++ ++QYS KG SRGK S RKAVMAP+ KL G++DEL KL DANMDDVS RRR REA
Sbjct: 25  RTKSSIQTQYSDKGGSRGKRSYRKAVMAPRKKLPGISDELQKLADANMDDVSARRRAREA 84

Query: 84  FKEIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDT 143
           FK IQLGIDH+LFKTP DGL+++ESYEVNSRGL IFSK WIPET RPKAMVCYCHGYGDT
Sbjct: 85  FKGIQLGIDHMLFKTPCDGLRIKESYEVNSRGLEIFSKSWIPETTRPKAMVCYCHGYGDT 144

Query: 144 CTFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVI 203
           CTFFFEGI            ARKLA+SGYGVF+MDYPGFGLSEGLHGFIP F+++VDDVI
Sbjct: 145 CTFFFEGI------------ARKLAMSGYGVFAMDYPGFGLSEGLHGFIPCFERLVDDVI 204

Query: 204 EHYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPW 263
           EHYSKVKENPEFSALPSF+FGQSLGGAVAL++HL QP SW GAVLVAPMCKIADDMVPPW
Sbjct: 205 EHYSKVKENPEFSALPSFIFGQSLGGAVALRIHLNQPRSWKGAVLVAPMCKIADDMVPPW 264

Query: 264 ALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRT 323
           ALAQ+LIGV+KFLPKYKLVPQ++LAEAAFRD+K+RELTA+NVIAY+HKPRL+TAVEML+T
Sbjct: 265 ALAQVLIGVSKFLPKYKLVPQRDLAEAAFRDLKYRELTAFNVIAYKHKPRLRTAVEMLKT 324

Query: 324 TQEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKA 383
           T+E+ RRL+E                      VSLP+LILHGEAD VTD +VSKALYEKA
Sbjct: 325 TEELGRRLRE----------------------VSLPILILHGEADIVTDVTVSKALYEKA 384

Query: 384 SSSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWLNEHCK 425
           SS+DK +KLY+ AYH+LLEGEPDE+I QVFDDIISWL+EHCK
Sbjct: 385 SSTDKKLKLYEGAYHALLEGEPDEMITQVFDDIISWLDEHCK 392

BLAST of Cp4.1LG01g22130 vs. TAIR 10
Match: AT5G16120.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.6e-140
Identity = 244/378 (64.55%), Postives = 299/378 (79.10%), Query Frame = 0

Query: 46  RKAVMAPKMKLHGVNDELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKM 105
           R+  MAP   L   N+EL +L + N+D+   RRRVR++ K+IQL +DHILFKTP +G+K 
Sbjct: 15  RRKSMAPTATLDN-NEELRRLREVNIDEAPGRRRVRDSLKDIQLNLDHILFKTPENGIKT 74

Query: 106 EESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIAR 165
           +ES+EVNSRG+ IFSK W+PE  +P+A+VC+CHGYGDTCTFFFE            GIAR
Sbjct: 75  KESFEVNSRGVEIFSKSWLPEASKPRALVCFCHGYGDTCTFFFE------------GIAR 134

Query: 166 KLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQ 225
           +LALSGYGVF+MDYPGFGLSEGLHG+IPSFD +V DVIEHYS +K NPEFS+LPSFLFGQ
Sbjct: 135 RLALSGYGVFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQ 194

Query: 226 SLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQK 285
           S+GGAV+LK+HLKQP++W GAVL+APMCKIADD+VPP  L QILIG+A  LPK+KLVPQK
Sbjct: 195 SMGGAVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQK 254

Query: 286 NLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLD 345
           +LAEA FRD++ R++T YN+I Y  KPRL+TAVEMLRTTQ+IE++L+E            
Sbjct: 255 DLAEAGFRDIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQE------------ 314

Query: 346 IPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEP 405
                     VSLP+LILHGEADTVTDPSVS+ LYEKA S DK I LY++AYHSLLEGEP
Sbjct: 315 ----------VSLPILILHGEADTVTDPSVSRELYEKAKSPDKKIVLYENAYHSLLEGEP 357

Query: 406 DEIIIQVFDDIISWLNEH 424
           D++I++V  DIISWLN+H
Sbjct: 375 DDMILRVLSDIISWLNDH 357

BLAST of Cp4.1LG01g22130 vs. TAIR 10
Match: AT5G16120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 495.7 bits (1275), Expect = 3.6e-140
Identity = 243/374 (64.97%), Postives = 297/374 (79.41%), Query Frame = 0

Query: 50  MAPKMKLHGVNDELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGLKMEESY 109
           MAP   L   N+EL +L + N+D+   RRRVR++ K+IQL +DHILFKTP +G+K +ES+
Sbjct: 1   MAPTATLDN-NEELRRLREVNIDEAPGRRRVRDSLKDIQLNLDHILFKTPENGIKTKESF 60

Query: 110 EVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIARKLAL 169
           EVNSRG+ IFSK W+PE  +P+A+VC+CHGYGDTCTFFFE            GIAR+LAL
Sbjct: 61  EVNSRGVEIFSKSWLPEASKPRALVCFCHGYGDTCTFFFE------------GIARRLAL 120

Query: 170 SGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQSLGG 229
           SGYGVF+MDYPGFGLSEGLHG+IPSFD +V DVIEHYS +K NPEFS+LPSFLFGQS+GG
Sbjct: 121 SGYGVFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQSMGG 180

Query: 230 AVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQKNLAE 289
           AV+LK+HLKQP++W GAVL+APMCKIADD+VPP  L QILIG+A  LPK+KLVPQK+LAE
Sbjct: 181 AVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQKDLAE 240

Query: 290 AAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLDIPFN 349
           A FRD++ R++T YN+I Y  KPRL+TAVEMLRTTQ+IE++L+E                
Sbjct: 241 AGFRDIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQE---------------- 300

Query: 350 PLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEPDEII 409
                 VSLP+LILHGEADTVTDPSVS+ LYEKA S DK I LY++AYHSLLEGEPD++I
Sbjct: 301 ------VSLPILILHGEADTVTDPSVSRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMI 339

Query: 410 IQVFDDIISWLNEH 424
           ++V  DIISWLN+H
Sbjct: 361 LRVLSDIISWLNDH 339

BLAST of Cp4.1LG01g22130 vs. TAIR 10
Match: AT1G77420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 6.0e-95
Identity = 182/397 (45.84%), Postives = 254/397 (63.98%), Query Frame = 0

Query: 29  TRSQYSYKGSSRGKSSSRKA---VMAPKMKLHGVNDELLKLLDANMDDVSTRRRVREAFK 88
           T S + Y+  S    + R A   V A +  + GV+DEL  +   N+D    RR  R AF 
Sbjct: 14  TLSPFRYRYGSSLAITRRFAPAIVCAKRSPIDGVSDELNLIASQNLDQAPARRLARSAFV 73

Query: 89  EIQLGIDHILFKTPSDGLKMEESYEVNSRGLSIFSKCWIPET-VRPKAMVCYCHGYGDTC 148
           ++QL +DH LFK    G++ EE YE NS+G  IF K W+P++    KA VC+CHGYG TC
Sbjct: 74  DLQLQLDHCLFKKAPSGIRTEEWYERNSKGEDIFCKSWLPKSGDEIKAAVCFCHGYGSTC 133

Query: 149 TFFFEGIYRLFCGICISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIE 208
           TFFF+            GIA+++A  GYGV+++D+PGFGLS+GLHG IPSFD + D+ IE
Sbjct: 134 TFFFD------------GIAKQIAGFGYGVYAIDHPGFGLSDGLHGHIPSFDDLADNAIE 193

Query: 209 HYSKVKENPEFSALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWA 268
            ++K+K   E   LP FL GQS+GGAVALK+HLK+P +W G +LVAPMCKI++D+ PP  
Sbjct: 194 QFTKMKGRSELRNLPRFLLGQSMGGAVALKIHLKEPQAWDGLILVAPMCKISEDVKPPPL 253

Query: 269 LAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTT 328
           + + LI ++   PK KL P+++L++  FRD+  R+L  Y+VI Y  + RL+TAVE+L  T
Sbjct: 254 VLKTLILMSTLFPKAKLFPKRDLSDFFFRDLSKRKLCEYDVICYDDQTRLKTAVELLNAT 313

Query: 329 QEIERRLKELENLFVTPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKAS 388
           ++IE ++ +                      VSLPLLILHG+ D VTDP+VSK L++ A 
Sbjct: 314 RDIEMQVDK----------------------VSLPLLILHGDTDKVTDPTVSKFLHKHAV 373

Query: 389 SSDKSIKLYKDAYHSLLEGEPDEIIIQVFDDIISWLN 422
           S DK++KLY   YH +LEG+ DE I  V +DI++WL+
Sbjct: 374 SQDKTLKLYPGGYHCILEGDTDENIFTVINDIVAWLD 376

BLAST of Cp4.1LG01g22130 vs. TAIR 10
Match: AT3G62860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-67
Identity = 136/316 (43.04%), Postives = 189/316 (59.81%), Query Frame = 0

Query: 106 EESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGICISGIAR 165
           EE Y  NSR + +F+  W+P +  P+A+V  CHGYG  C+ F        CGI       
Sbjct: 9   EEEYIKNSRDVELFACRWLPSS-SPRALVFLCHGYGMECSSFMRE-----CGI------- 68

Query: 166 KLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALPSFLFGQ 225
           +LA +GY VF MDY G G S+G   +I  F  IV+D  ++Y+ +    E+     FL+G+
Sbjct: 69  RLASAGYAVFGMDYEGHGRSKGARCYIKKFSNIVNDCFDYYTSISAQEEYKEKGRFLYGE 128

Query: 226 SLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKYKLVPQK 285
           S+GGAVAL +H K P  W GA+LVAPMCKI++ + P   +  +L  V   +PK+K+VP K
Sbjct: 129 SMGGAVALLLHKKDPSFWNGALLVAPMCKISEKVKPHPVVINLLTRVEDIIPKWKIVPTK 188

Query: 286 NLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFVTPWLLD 345
           ++ +AAF+D   RE    N + YQ KPRL+TA+EMLRT+ ++E  L E            
Sbjct: 189 DVIDAAFKDPVKREEIRNNKLIYQDKPRLKTALEMLRTSMDLEDTLHE------------ 248

Query: 346 IPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLEGEP 405
                     ++LP  +LHGEAD VTDP +SKAL+EKAS+ DK+IKLY   +H L  GEP
Sbjct: 249 ----------ITLPFFVLHGEADIVTDPEISKALFEKASTRDKTIKLYPGMWHGLTSGEP 289

Query: 406 DEIIIQVFDDIISWLN 422
           D  +  VF DI++WL+
Sbjct: 309 DANVDLVFADIVNWLD 289

BLAST of Cp4.1LG01g22130 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-67
Identity = 133/323 (41.18%), Postives = 193/323 (59.75%), Query Frame = 0

Query: 100 SDGLKMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIYRLFCGIC 159
           ++ +K EES+  N+RG+ +F+  W+P    PKA+V  CHGY             + C I 
Sbjct: 5   TENIKYEESFIKNTRGMKLFTCKWVPAKQEPKALVFICHGYA------------MECSIT 64

Query: 160 ISGIARKLALSGYGVFSMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVKENPEFSALP 219
           ++  AR+L  +G+ V+ +DY G G S+GL  ++P+FD +VDDV  HY+ + E  E     
Sbjct: 65  MNSTARRLVKAGFAVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKM 124

Query: 220 SFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMVPPWALAQILIGVAKFLPKY 279
            FL G+S+GGAV L +H K+P  W GAVLVAPMCKIA++M P   +  IL  ++  +P +
Sbjct: 125 RFLLGESMGGAVLLLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSW 184

Query: 280 KLVPQKNLAEAAFRDMKHRELTAYNVIAYQHKPRLQTAVEMLRTTQEIERRLKELENLFV 339
           K++P +++ E AF+  + R+    N   Y+ +PRL+TA E+LR + ++E+RL E      
Sbjct: 185 KIIPGQDIIETAFKQPEIRKQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNE------ 244

Query: 340 TPWLLDIPFNPLFDTLVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHS 399
                           VSLP ++LHGE D VTD +VS+ LYE ASSSDK+ KLY   +H 
Sbjct: 245 ----------------VSLPFIVLHGEDDKVTDKAVSRQLYEVASSSDKTFKLYPGMWHG 293

Query: 400 LLEGEPDEIIIQVFDDIISWLNE 423
           LL GE  E I  VF DII WL++
Sbjct: 305 LLYGETPENIETVFADIIGWLDK 293