Cp4.1LG01g15400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g15400
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPectin lyase-like superfamily protein
LocationCp4.1LG01: 9199188 .. 9201919 (-)
RNA-Seq ExpressionCp4.1LG01g15400
SyntenyCp4.1LG01g15400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGTTTCGTATGTTATTATTTATGAGTATTTGGAGACTTTTTCTCCACATTTTAAATGTACAGAAACTATTTTCACCATGCATAGAAGAAGCTTCAGAGTTGAGATTGCTCAGAAAAAAGAAATTGTGAAATGAGCCCAAAACAAAGAGAGAGGGACGGGGGAGAAACAAAACAAAACGCCTCAATGGCGGTGTTGAATTTGGTGTGCTGGTTTTAGAGTAAAGCAAAGAAACTTCAAGTTTTTCTCTTCTACTTTACGTGTAGGCATATTGGGAAATGGCGACCTCCCCTTAATCTGCTTTCAAGCAAAGCCATATTGCCCACTAAAATGGCTACTTTATTTTCCCTTATAAATTCTAACCTCTTTCATTGCATCATTGTATTAACACAAATTACTCTACTAATCTCTTCTACCAAGTCGACTGAAAATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGATCATTGTCTATGGAGTTTCAGGGAGTATCGTGCCTGATGGCAACATTGACATGGTTAAAGAACTCGAAAGTTTCGACATTGAAGAAGAGGACGACAGTGCGGTGGACACATATGCGGTACCGGTGTGGAGAAGTGAGCACGGGAGCAAGATTCTGATAAATGTGGATAGTTTTGACGCGGTTGGAGATGGAGTTACAGATGACACTATGGTACATAGATATTTCTGTTCGGAAATATGGATTTTGCAGAGACAATACTGAACTGAAGTGTTTATTTTGCAATCAGGCTTTCAGGAAGGCATGGGAGACTGCTTGCAGCTCTTCAAATTCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACTAAGTTTAAAGGGCCATGCAAACATGGTATGGTAATCCAGGTAAGTCATAGGATTATAAGGATTCTTTTCCCCCAAACATATAAGGAGGGAAGATCATGGTTGAAAGTTAAGAGTTGACATTGCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAATTGGGATCCAAAACTTCCCCGTCAATGGCTGGAGTTTACAAAACTAAGTGCAGCTACCTTTCAAGGGGATGGAATTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGGTAAATTCTTGAACCATCTCTTAGCTTGTTTCTCTAGCTATGTTATTGAAATCTTAATCATTTCTGGTTTTCTGGTTGCTTTTTTTCCAGCCTTGCAAAGGAGCACCAACAGTAAGAACAAAACTACTTTCCTCCCAGTGGAAACCATGATTTTTCCTTGTGTAACACATTCTGATTTGAGGGATTTCTTGCAGGCATTAACCATAGAGTCGAGTTCAAATATAAGAGTAAAAGGCCTAACCATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTAAAACTGTTCGAGTTTCGGAGGTAAGAGTGTCAGCTCCAGGAGATAGCCCCAACACCGACGGGATCCACATCACGCAATCAACTAATGTCGTAGTTGAAAACTGCAAGATTTCAACAGGTTTTCTTCATTCTGACATTCATCATGTTACCTTTAACACCACTATTTCACCCTCTAACAGGTTATATGCAGGTGATGACTGCATCTCCATTGTCAATGCTAGCTCTGGCATCAAGATGAAGAGAATATCATGTGGACCAGGACATGGGATCAGGTATCTGCAAATGTTTGCATTTATTTCAAGCACCAAAACCATATCAATGTCTTAAGAATTCCTTTCTCTTTTTGTTTGTGTTCCTTAGCATTGGAAGTTTAGGGAAAGACAACTCCACTGGTATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACCTGGCAGGTTAGAATCACATCTTTAACCACCATGTTGGTACTTACTTTTACCAAGAACAAGGGGAAACTCTGTCTGTGCATATGGTACAGGGAGGTTCGGGATATGTTCGCGCCGTTCGGTTCGAAAATGTGAGAATGGAAAATGTTGAGAACCCCATTATCATCGATCAGTTCTACTGTGACCATACTACTTGTGAACCTCAGGTATTACCCTCAAAATGCAGAATTTAGTTTGTCCATTATCCATCATGGAGTCCAATACACTATTTTCTGTCAGGCATCTGCCGTGAAAATAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAGTTCGCCTGCAGCGACAGCTTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAGACCTACTGCCACGCCGCTCAAGGCTTTGGATATGGCGTGGTGCATCCATCAGCAGACTGCTTAAGTTCCAGCGACAATGACATTGCAGCTTCAGATGAGACCCAAGTTCCGGAGCTGGAGGCAGTGCAAATCCCTACTGAGCGGCACATAGTTCACACTGAGCTCTGACCTTCATGTCCTGTCCATCTAAACTAAACTCAGCAAATATTTGTAGCATATATACATAGCACTCACAATTATACCTACCCTTTTGGAATCTAAATAGAAATATAGACTACCAAGTAAATGATTGTAATATTTCTTGAAGAAAGCATACATATTTTGAATTTATAGTAATGGTTTTTTAATACCAGAGTTCATTGCATATGGTTTATGGCCTTCCAGTTCTAAGTATATATAATACACACAAATGC

mRNA sequence

TGGGTTTCGTATGTTATTATTTATGAGTATTTGGAGACTTTTTCTCCACATTTTAAATGTACAGAAACTATTTTCACCATGCATAGAAGAAGCTTCAGAGTTGAGATTGCTCAGAAAAAAGAAATTGTGAAATGAGCCCAAAACAAAGAGAGAGGGACGGGGGAGAAACAAAACAAAACGCCTCAATGGCGGTGTTGAATTTGGTGTGCTGGTTTTAGAGTAAAGCAAAGAAACTTCAAGTTTTTCTCTTCTACTTTACGTGTAGGCATATTGGGAAATGGCGACCTCCCCTTAATCTGCTTTCAAGCAAAGCCATATTGCCCACTAAAATGGCTACTTTATTTTCCCTTATAAATTCTAACCTCTTTCATTGCATCATTGTATTAACACAAATTACTCTACTAATCTCTTCTACCAAGTCGACTGAAAATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGATCATTGTCTATGGAGTTTCAGGGAGTATCGTGCCTGATGGCAACATTGACATGGTTAAAGAACTCGAAAGTTTCGACATTGAAGAAGAGGACGACAGTGCGGTGGACACATATGCGGTACCGGTGTGGAGAAGTGAGCACGGGAGCAAGATTCTGATAAATGTGGATAGTTTTGACGCGGTTGGAGATGGAGTTACAGATGACACTATGGCTTTCAGGAAGGCATGGGAGACTGCTTGCAGCTCTTCAAATTCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACTAAGTTTAAAGGGCCATGCAAACATGGTATGGTAATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAATTGGGATCCAAAACTTCCCCGTCAATGGCTGGAGTTTACAAAACTAAGTGCAGCTACCTTTCAAGGGGATGGAATTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGCCTTGCAAAGGAGCACCAACAGCATTAACCATAGAGTCGAGTTCAAATATAAGAGTAAAAGGCCTAACCATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTAAAACTGTTCGAGTTTCGGAGGTAAGAGTGTCAGCTCCAGGAGATAGCCCCAACACCGACGGGATCCACATCACGCAATCAACTAATGTCGTAGTTGAAAACTGCAAGATTTCAACAGGGAAAGACAACTCCACTGGTATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACCTGGCAGGGAGGTTCGGGATATGTTCGCGCCGTTCGGTTCGAAAATGTGAGAATGGAAAATGTTGAGAACCCCATTATCATCGATCAGTTCTACTGTGACCATACTACTTGTGAACCTCAGGCATCTGCCGTGAAAATAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAGTTCGCCTGCAGCGACAGCTTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAGACCTACTGCCACGCCGCTCAAGGCTTTGGATATGGCGTGGTGCATCCATCAGCAGACTGCTTAAGTTCCAGCGACAATGACATTGCAGCTTCAGATGAGACCCAAGTTCCGGAGCTGGAGGCAGTGCAAATCCCTACTGAGCGGCACATAGTTCACACTGAGCTCTGACCTTCATGTCCTGTCCATCTAAACTAAACTCAGCAAATATTTGTAGCATATATACATAGCACTCACAATTATACCTACCCTTTTGGAATCTAAATAGAAATATAGACTACCAAGTAAATGATTGTAATATTTCTTGAAGAAAGCATACATATTTTGAATTTATAGTAATGGTTTTTTAATACCAGAGTTCATTGCATATGGTTTATGGCCTTCCAGTTCTAAGTATATATAATACACACAAATGC

Coding sequence (CDS)

ATGGATAAATTCTTCCTGCTTTCCCTTCTTGGTATGCTGATCATTGTCTATGGAGTTTCAGGGAGTATCGTGCCTGATGGCAACATTGACATGGTTAAAGAACTCGAAAGTTTCGACATTGAAGAAGAGGACGACAGTGCGGTGGACACATATGCGGTACCGGTGTGGAGAAGTGAGCACGGGAGCAAGATTCTGATAAATGTGGATAGTTTTGACGCGGTTGGAGATGGAGTTACAGATGACACTATGGCTTTCAGGAAGGCATGGGAGACTGCTTGCAGCTCTTCAAATTCAGTTCTTTTGGTGCCAAAAGAAGGGAGATATCTGGTGAATGCAACTAAGTTTAAAGGGCCATGCAAACATGGTATGGTAATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGAATTGGGATCCAAAACTTCCCCGTCAATGGCTGGAGTTTACAAAACTAAGTGCAGCTACCTTTCAAGGGGATGGAATTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGTAAAAAGAACAAGTCCAGGCCTTGCAAAGGAGCACCAACAGCATTAACCATAGAGTCGAGTTCAAATATAAGAGTAAAAGGCCTAACCATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTAAAACTGTTCGAGTTTCGGAGGTAAGAGTGTCAGCTCCAGGAGATAGCCCCAACACCGACGGGATCCACATCACGCAATCAACTAATGTCGTAGTTGAAAACTGCAAGATTTCAACAGGGAAAGACAACTCCACTGGTATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACCTGGCAGGGAGGTTCGGGATATGTTCGCGCCGTTCGGTTCGAAAATGTGAGAATGGAAAATGTTGAGAACCCCATTATCATCGATCAGTTCTACTGTGACCATACTACTTGTGAACCTCAGGCATCTGCCGTGAAAATAAGCCAAATCATGTACCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAGTTCGCCTGCAGCGACAGCTTTCCATGCAGCAACATAATTCTAAGCAATGTCAATTTGGAGAAGACGGATGGAACAGTGGAGACCTACTGCCACGCCGCTCAAGGCTTTGGATATGGCGTGGTGCATCCATCAGCAGACTGCTTAAGTTCCAGCGACAATGACATTGCAGCTTCAGATGAGACCCAAGTTCCGGAGCTGGAGGCAGTGCAAATCCCTACTGAGCGGCACATAGTTCACACTGAGCTCTGA

Protein sequence

MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEHGSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCKHGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKNKSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGIHITQSTNVVVENCKISTGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEPQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL
Homology
BLAST of Cp4.1LG01g15400 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.0e-86
Identity = 169/392 (43.11%), Postives = 235/392 (59.95%), Query Frame = 0

Query: 62  SKILINVDSFDAVGDGVTDDTMAFRKAWETACSSS-NSVLLVPKEGRYLVNATKFKGPCK 121
           S+ L++V +F A G+GVTDDT AF  AW+TACSS   + +LVP+    L+      GPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 122 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 181
             + +QI GTI+AP++P+ W+    R+WL F  LS  T +G G ++G G++WW  SCK N
Sbjct: 109 ARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHN 168

Query: 182 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 241
            S PC+GAPTALT     N+RV+ L + +SQQMH  +   + V +S ++V AP  SPNTD
Sbjct: 169 HSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTD 228

Query: 242 GIHITQSTNVVVENCKISTGKD------NSTGI--------------------------V 301
           GIHI+ S  +V++N  +STG D      NST I                          V
Sbjct: 229 GIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEV 288

Query: 302 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCD-HTTCE 361
             + +DTA + +T NGVRIKTWQGGSG V  + F N++M NV NPIIIDQ+YCD    C 
Sbjct: 289 RDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCA 348

Query: 362 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKT--DGTVETYCH 418
            Q SA+ I  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C 
Sbjct: 349 NQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 408

BLAST of Cp4.1LG01g15400 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.1e-79
Identity = 166/367 (45.23%), Postives = 216/367 (58.86%), Query Frame = 0

Query: 66  INVDSFDAVGDGVTDDTMAFRKAWETACSS-SNSVLLVPKEGRYLVNATKFKGPCKHGMV 125
           +NVD F A GDG  DDT AF KAW+ ACSS S++VLLVPK+  YLV    F GPCK G+ 
Sbjct: 90  VNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKK-NYLVRPISFSGPCKSGLT 149

Query: 126 IQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKNKSRP 185
           +QI GTI A D+  ++  K  R WL F  +     +G G I+G+G+ WW  SCK NK+ P
Sbjct: 150 MQIYGTIEASDDRSDY-RKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALP 209

Query: 186 CKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGIHI 245
           CK APTALT   S ++ VK L I+N+QQ+H        V+ S + V+AP +SPNTDGIH+
Sbjct: 210 CKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHV 269

Query: 246 TQSTNVVVENCKISTGKD--------------------------------NSTGIVTKVV 305
           T + N+ + +C I TG D                                NS   V+ VV
Sbjct: 270 TGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVV 329

Query: 306 LDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCEPQAS 365
           ++ A L  TTNGVRIKTWQGGSG    ++F+NV M NVENPIIIDQ YCD    C+ Q+S
Sbjct: 330 VNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSS 389

Query: 366 AVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDG-TVETYCHAAQGF 398
           AV++  ++Y+NI GT  S  A+ F CS  FPC  I+L +V+LE   G   +  C+  +  
Sbjct: 390 AVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELS 449

BLAST of Cp4.1LG01g15400 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.1e-77
Identity = 160/362 (44.20%), Postives = 207/362 (57.18%), Query Frame = 0

Query: 54  PVWRSEHGSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNAT 113
           PV   E      I+VD F A GDG TDDT AF KAW+ AC SS SVL+VP+   YL+   
Sbjct: 73  PVGGPETSPDTDISVDDFGARGDG-TDDTKAFEKAWKDAC-SSGSVLIVPENKNYLLKQI 132

Query: 114 KFKGPCKHGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWW 173
            F GPCK  + ++I GTI A  +  +W     ++W+EF  +S  T +G G  +G+GE WW
Sbjct: 133 TFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW 192

Query: 174 AESCKKNKSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAP 233
             SCK+ KS PCK APTALT  S  N+ V  L+I++SQ+MH    + + V  S + V+AP
Sbjct: 193 DSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAP 252

Query: 234 GDSPNTDGIHITQSTNVVVENCKISTGKD------------------------------- 293
             SPNTDGIHIT +  + V N  I TG D                               
Sbjct: 253 EHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGD 312

Query: 294 -NSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYC 353
            NS   V+ V++D   L +TTNG+RIKTWQGGSG  + ++F+N+ M NV NPIIIDQ+YC
Sbjct: 313 RNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYC 372

Query: 354 D-HTTCEPQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTV 383
           D    C  Q SAVK+S + Y NI GT+ S+ A+KF CS S PC   I+ N+NL    G  
Sbjct: 373 DSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKE 432

BLAST of Cp4.1LG01g15400 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.2e-76
Identity = 153/365 (41.92%), Postives = 213/365 (58.36%), Query Frame = 0

Query: 66  INVDSFDAVGDGVTDDTMAFRKAWETACSSSN-SVLLVPKEGRYLVNATKFKGPCKHGMV 125
           ++V +F A GDG TDDT AF+KAW+ ACS++  +  LVPK   YL+ +T+F+GPCK    
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRN 127

Query: 126 IQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQG--DGIIDGSGEKWWAESCKKNKS 185
            QI GT+ A  +  ++  K    WL    ++  +  G   GII+G+G+ WW  SCK +KS
Sbjct: 128 FQILGTLSASTKRSDYKDK--NHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS 187

Query: 186 RPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGI 245
           +PC  APTALT+ +  N+ VK L ++N+QQ+   I +   V VS V ++APGDSPNTDGI
Sbjct: 188 KPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGI 247

Query: 246 HITQSTNVVVENCKIST--------------------------------GKDNSTGIVTK 305
           HIT + N+ V N  I T                                G DNS   V+ 
Sbjct: 248 HITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSG 307

Query: 306 VVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEPQA 365
           + +D A   E+ NGVRIKT+QGGSG  + ++F+N+RMENV+NPIIIDQ YCD   CE Q 
Sbjct: 308 INVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCEDQE 367

Query: 366 SAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLE-----------KTDGT 385
           SAV++  ++Y+NISGT+ +  A+   CS+ +PC  I+L NV ++           K  GT
Sbjct: 368 SAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKNQGT 427

BLAST of Cp4.1LG01g15400 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 2.6e-74
Identity = 154/370 (41.62%), Postives = 214/370 (57.84%), Query Frame = 0

Query: 66  INVDSFDAVGDGVTDDTMAFRKAWETACSSSNSV-LLVPKEGRYLVNATKFKGPCKHGMV 125
           ++V  F A GDG TDDT AF  AW+ ACSS+ +V LLVPK   YL+ + +  GPC   + 
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 127

Query: 126 IQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQG--DGIIDGSGEKWWAESCKKNKS 185
           +QI GT+ A  +    D K   +W+ F  ++  +  G   G++DG+GE WW  SCK+NK+
Sbjct: 128 VQIFGTLSASQKRS--DYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKA 187

Query: 186 RPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGI 245
           +PC  APTALT  +S ++ VK L ++N+QQ+   I +   V+VS V V+AP DSPNTDGI
Sbjct: 188 KPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGI 247

Query: 246 HITQSTNVVVENCKIST--------------------------------GKDNSTGIVTK 305
           HIT + N+ V    I T                                G DNS   V+ 
Sbjct: 248 HITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSG 307

Query: 306 VVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEPQA 365
           V +D A L  T NGVRIKT+QGGSG    + F+N++M+NV+NPIIIDQ YCD + C  + 
Sbjct: 308 VTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEK 367

Query: 366 SAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGF 401
           SAV++  ++YR+ISGT+ S+NA+ F CS ++PC  I+L  VN++    T    C  A   
Sbjct: 368 SAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKGGKAT----CTNANVV 427

BLAST of Cp4.1LG01g15400 vs. NCBI nr
Match: KAG6601508.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 870 bits (2248), Expect = 0.0
Identity = 432/436 (99.08%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLLSLLGMLIIVYGVSGSI+PDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIMPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
           HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKL+AATFQGDGIIDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAV 300
           GIHITQSTNVVVENCKISTGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAV
Sbjct: 241 GIHITQSTNVVVENCKISTGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAV 300

Query: 301 RFENVRMENVENPIIIDQFYCDHTTCEPQASAVKISQIMYRNISGTTTSKNAMKFACSDS 360
           RFENVRMENVENPIIIDQFYCDHTTCEPQASAVKISQIMYRNISGTTTSKNAMKFACSDS
Sbjct: 301 RFENVRMENVENPIIIDQFYCDHTTCEPQASAVKISQIMYRNISGTTTSKNAMKFACSDS 360

Query: 361 FPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDNDIAASDETQVPEL 420
           FPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDNDIAASDETQVPEL
Sbjct: 361 FPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLSSSDNDIAASDETQVPEL 420

Query: 421 EAVQIPTERHIVHTEL 436
           EAVQ+PTERHIVHTEL
Sbjct: 421 EAVQLPTERHIVHTEL 436

BLAST of Cp4.1LG01g15400 vs. NCBI nr
Match: XP_022977184.1 (probable polygalacturonase At1g80170 [Cucurbita maxima])

HSP 1 Score: 852 bits (2202), Expect = 4.28e-311
Identity = 433/468 (92.52%), Postives = 435/468 (92.95%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLLSLLGMLIIVYGVSGSIVPDG+IDMVKELESFDIEEEDDSAVDTYAVPVWRSEH
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
           HGMVIQIEGTIVAP EPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPGEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVVENCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360

Query: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420
           QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ
Sbjct: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420

Query: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQ+PTERHIVHTEL
Sbjct: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQLPTERHIVHTEL 468

BLAST of Cp4.1LG01g15400 vs. NCBI nr
Match: XP_022956901.1 (probable polygalacturonase At1g80170 [Cucurbita moschata])

HSP 1 Score: 851 bits (2199), Expect = 1.22e-310
Identity = 431/468 (92.09%), Postives = 435/468 (92.95%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLLSLLGMLIIVYGVSGSIVPDG+IDMVKELESFDIEEEDDSAVDTYAVP WRSEH
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPAWRSEH 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
           HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKL+AATFQGDGIIDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVVENCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360

Query: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420
           QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ
Sbjct: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420

Query: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQ+PTERHIVHTEL
Sbjct: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQLPTERHIVHTEL 468

BLAST of Cp4.1LG01g15400 vs. NCBI nr
Match: XP_011655619.1 (probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus])

HSP 1 Score: 758 bits (1958), Expect = 6.59e-274
Identity = 385/469 (82.09%), Postives = 408/469 (86.99%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDK FLLSLLG+ II YGVSG+ V D +IDM+KELESFDIEEEDD  V +Y VPVWRSE 
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKIL+NVDSFDA+GDGVTDDTMAFRKAWETACS+S SVLLVPKE  YLVNAT FKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHF IARS+TVR++ VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVV+NCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360
           TKVVLDTA+LRETTNGVRIKTWQGGSGYVRAVRFENVRME+VENPIIIDQFYCD  TTCE
Sbjct: 301 TKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE 360

Query: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SAVKISQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 SQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           QGFGYGVVHPSADCL+SSDN+ A+SD+TQV ELE VQ+ TE+HIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL 469

BLAST of Cp4.1LG01g15400 vs. NCBI nr
Match: XP_008446196.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 759 bits (1960), Expect = 1.29e-273
Identity = 385/469 (82.09%), Postives = 408/469 (86.99%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDK FLLSLLG+LII YGVSG+ V D +IDM++ELESFDIEEEDD  V +Y VP WRSE 
Sbjct: 38  MDKLFLLSLLGLLIIAYGVSGTFVSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSER 97

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKIL+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKE RYLVNAT FKGPCK
Sbjct: 98  GSKILMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCK 157

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 158 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTA TI+SSSNIRVKGLTIQNSQQMHF IARS+TVR++EVRVSAPGDSPNTD
Sbjct: 218 KSRPCKGAPTAFTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTD 277

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVV+N KISTG                                KDNSTGIV
Sbjct: 278 GIHITQSTNVVVQNSKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 337

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRME+VENPIIIDQFYCD  TTCE
Sbjct: 338 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 397

Query: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SAVKISQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 398 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 457

Query: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           QGFGYG+VHPSADCL+SSDN+ AA D+TQV ELE VQ+PTE+HIVHTEL
Sbjct: 458 QGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIVHTEL 506

BLAST of Cp4.1LG01g15400 vs. ExPASy TrEMBL
Match: A0A6J1IQQ8 (probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111477357 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 2.07e-311
Identity = 433/468 (92.52%), Postives = 435/468 (92.95%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLLSLLGMLIIVYGVSGSIVPDG+IDMVKELESFDIEEEDDSAVDTYAVPVWRSEH
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
           HGMVIQIEGTIVAP EPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPGEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVVENCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360

Query: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420
           QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ
Sbjct: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420

Query: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQ+PTERHIVHTEL
Sbjct: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQLPTERHIVHTEL 468

BLAST of Cp4.1LG01g15400 vs. ExPASy TrEMBL
Match: A0A6J1GXR9 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111458447 PE=3 SV=1)

HSP 1 Score: 851 bits (2199), Expect = 5.93e-311
Identity = 431/468 (92.09%), Postives = 435/468 (92.95%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLLSLLGMLIIVYGVSGSIVPDG+IDMVKELESFDIEEEDDSAVDTYAVP WRSEH
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPAWRSEH 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
           HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKL+AATFQGDGIIDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVVENCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEP 360

Query: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420
           QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ
Sbjct: 361 QASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQ 420

Query: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQ+PTERHIVHTEL
Sbjct: 421 GFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQLPTERHIVHTEL 468

BLAST of Cp4.1LG01g15400 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 3.19e-274
Identity = 385/469 (82.09%), Postives = 408/469 (86.99%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDK FLLSLLG+ II YGVSG+ V D +IDM+KELESFDIEEEDD  V +Y VPVWRSE 
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKIL+NVDSFDA+GDGVTDDTMAFRKAWETACS+S SVLLVPKE  YLVNAT FKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHF IARS+TVR++ VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVV+NCKISTG                                KDNSTGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360
           TKVVLDTA+LRETTNGVRIKTWQGGSGYVRAVRFENVRME+VENPIIIDQFYCD  TTCE
Sbjct: 301 TKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE 360

Query: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SAVKISQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 SQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           QGFGYGVVHPSADCL+SSDN+ A+SD+TQV ELE VQ+ TE+HIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL 469

BLAST of Cp4.1LG01g15400 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 6.25e-274
Identity = 385/469 (82.09%), Postives = 408/469 (86.99%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDK FLLSLLG+LII YGVSG+ V D +IDM++ELESFDIEEEDD  V +Y VP WRSE 
Sbjct: 38  MDKLFLLSLLGLLIIAYGVSGTFVSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSER 97

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GSKIL+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKE RYLVNAT FKGPCK
Sbjct: 98  GSKILMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCK 157

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 158 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTA TI+SSSNIRVKGLTIQNSQQMHF IARS+TVR++EVRVSAPGDSPNTD
Sbjct: 218 KSRPCKGAPTAFTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTD 277

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQSTNVVV+N KISTG                                KDNSTGIV
Sbjct: 278 GIHITQSTNVVVQNSKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 337

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRME+VENPIIIDQFYCD  TTCE
Sbjct: 338 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 397

Query: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SAVKISQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 398 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 457

Query: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           QGFGYG+VHPSADCL+SSDN+ AA D+TQV ELE VQ+PTE+HIVHTEL
Sbjct: 458 QGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIVHTEL 506

BLAST of Cp4.1LG01g15400 vs. ExPASy TrEMBL
Match: A0A6J1FZ30 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111449225 PE=3 SV=1)

HSP 1 Score: 741 bits (1912), Expect = 2.40e-267
Identity = 370/469 (78.89%), Postives = 403/469 (85.93%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSEH 60
           MDKFFLL LL +LII YGVSGSI P+ +IDMVKELE+FDIEE+DD+ VDTYA+PVW+SE 
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120
           GS+ L+NVDSFDAVGDG+TDDTMAFRKAWETACS++ SVLLVPKE RYLVNAT FKGPC 
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 180
            GM+IQIEGTI+APDEPE WDPKLPRQWLEFTKL+AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240
           KSRPCKGAPTALTI+SSSNIRVKGLTIQNSQQMHF IARS+TVR++EVRVS+PGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVENCKISTG--------------------------------KDNSTGIV 300
           GIHITQS NVVV+NCKISTG                                KDNS GIV
Sbjct: 241 GIHITQSMNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360
           +KVVLDTAFLRETTNGVRIKTWQGGSGYVRA+RFENVRM++VENPIIIDQFYCD    CE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAIRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SA+K+SQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 436
           QGFGYGVVHPSADCL+SSDND AA D+TQ        +PTE+HIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQTQ--------LPTEQHIVHTEL 461

BLAST of Cp4.1LG01g15400 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 548.9 bits (1413), Expect = 3.7e-156
Identity = 275/441 (62.36%), Postives = 331/441 (75.06%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLII-VYGVSGSIVPDGNIDMVKELESFDIEEEDDSAVDTYAVPVWRSE 60
           MDK F+LSLLG+L++  YG +G +V   ++D++ ELE+FD+  +DD        P + S 
Sbjct: 2   MDKLFILSLLGLLLVTAYGAAGKMVYT-DLDILDELENFDVLVDDDDDTKLLDWPSFTSR 61

Query: 61  HGSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPC 120
           H  K L+NVD+F A GDGV+DDT AF  AW  ACS+S SV LVP+  RYLVNATKF GPC
Sbjct: 62  HSGKNLVNVDTFGAAGDGVSDDTQAFVSAWSKACSTSKSVFLVPEGRRYLVNATKFNGPC 121

Query: 121 KHGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKK 180
           +  ++IQI+GTIVAPDEP NWD K  R WLEF+KL    FQG G+IDGSG KWWA SCKK
Sbjct: 122 EQKLIIQIDGTIVAPDEPSNWDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKK 181

Query: 181 NKSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNT 240
           NKS PCK APTALTIESSS ++V GLTIQNSQQM+FIIARS +VRVS+V VS+PGDSPNT
Sbjct: 182 NKSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNT 241

Query: 241 DGIHITQSTNVVVENCKIST--------------------------------GKDNSTGI 300
           DGIHIT STNV++++CKI T                                GKDN+TGI
Sbjct: 242 DGIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGI 301

Query: 301 VTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTC 360
           VT+VVLDTA LRETTNG+RIKT+QGGSGYV+ +RF NV M++V NPI+IDQFYCD  TTC
Sbjct: 302 VTQVVLDTALLRETTNGLRIKTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTC 361

Query: 361 EPQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHA 408
           + Q SAVKISQIMYRNI+GTT S  A+KFACSD+ PCS+I+L+NVNLE  DG VE YC++
Sbjct: 362 QNQTSAVKISQIMYRNITGTTKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVEAYCNS 421

BLAST of Cp4.1LG01g15400 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-146
Identity = 264/471 (56.05%), Postives = 335/471 (71.13%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIV-YGVSGSIVPDGNIDMVKELESFDIEEED-DSAVDTYAVPVWRS 60
           M K F++SL  +L++  YG +G ++    +D++ +LE+ D+ E+D +  V  +    + S
Sbjct: 1   MIKPFIISLFALLMVASYGATGKML-FKELDILGKLENLDVPEDDIEDDVTFFDFSSFTS 60

Query: 61  EHGSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGP 120
           ++  K L+NVDSF+A GDGV+DDT AF +AW  ACS+ NSVLLVP+   YLVNATKF GP
Sbjct: 61  QYSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFDGP 120

Query: 121 CKHGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCK 180
           C+  ++IQI+GTI+APDEP  WDPK PR WL+F+KL    FQG+G+IDGSG KWWA SCK
Sbjct: 121 CQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCK 180

Query: 181 KNKSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPN 240
           KNKS PC GAPTALTI SSSN+ V+GLTI+NSQQMH II RS TVR+S V V++PGDSPN
Sbjct: 181 KNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPN 240

Query: 241 TDGIHITQSTNVVVENCKISTGKD--------------------------------NSTG 300
           TDGIHIT ST+VVV++ KISTG D                                +S G
Sbjct: 241 TDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHSKG 300

Query: 301 IVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TT 360
            VT VVL+TAFL+ TTNG+RIKTWQGG+GYV+ VRFENV M++V NPIIIDQFYCD  +T
Sbjct: 301 TVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPST 360

Query: 361 CEPQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCH 420
           C+ Q SAV IS+IMYRNI+GTT S  A+ F CSD+ PCS+I+L+N+NLE  DG VE YC+
Sbjct: 361 CQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVEAYCN 420

Query: 421 AAQGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQIPTERHIVHTEL 437
           +A+GFGYGVVHPSADCL S D+   + ++T     E + +  E    H EL
Sbjct: 421 SAEGFGYGVVHPSADCLYSHDD--KSLNQTDQYLAETIMVEKETENAHDEL 468

BLAST of Cp4.1LG01g15400 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 7.3e-88
Identity = 169/392 (43.11%), Postives = 235/392 (59.95%), Query Frame = 0

Query: 62  SKILINVDSFDAVGDGVTDDTMAFRKAWETACSSS-NSVLLVPKEGRYLVNATKFKGPCK 121
           S+ L++V +F A G+GVTDDT AF  AW+TACSS   + +LVP+    L+      GPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 122 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQGDGIIDGSGEKWWAESCKKN 181
             + +QI GTI+AP++P+ W+    R+WL F  LS  T +G G ++G G++WW  SCK N
Sbjct: 109 ARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHN 168

Query: 182 KSRPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 241
            S PC+GAPTALT     N+RV+ L + +SQQMH  +   + V +S ++V AP  SPNTD
Sbjct: 169 HSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTD 228

Query: 242 GIHITQSTNVVVENCKISTGKD------NSTGI--------------------------V 301
           GIHI+ S  +V++N  +STG D      NST I                          V
Sbjct: 229 GIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEV 288

Query: 302 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCD-HTTCE 361
             + +DTA + +T NGVRIKTWQGGSG V  + F N++M NV NPIIIDQ+YCD    C 
Sbjct: 289 RDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCA 348

Query: 362 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKT--DGTVETYCH 418
            Q SA+ I  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  E++C 
Sbjct: 349 NQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 408

BLAST of Cp4.1LG01g15400 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.5e-77
Identity = 153/365 (41.92%), Postives = 213/365 (58.36%), Query Frame = 0

Query: 66  INVDSFDAVGDGVTDDTMAFRKAWETACSSSN-SVLLVPKEGRYLVNATKFKGPCKHGMV 125
           ++V +F A GDG TDDT AF+KAW+ ACS++  +  LVPK   YL+ +T+F+GPCK    
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRN 127

Query: 126 IQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQG--DGIIDGSGEKWWAESCKKNKS 185
            QI GT+ A  +  ++  K    WL    ++  +  G   GII+G+G+ WW  SCK +KS
Sbjct: 128 FQILGTLSASTKRSDYKDK--NHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKS 187

Query: 186 RPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGI 245
           +PC  APTALT+ +  N+ VK L ++N+QQ+   I +   V VS V ++APGDSPNTDGI
Sbjct: 188 KPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGI 247

Query: 246 HITQSTNVVVENCKIST--------------------------------GKDNSTGIVTK 305
           HIT + N+ V N  I T                                G DNS   V+ 
Sbjct: 248 HITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSG 307

Query: 306 VVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEPQA 365
           + +D A   E+ NGVRIKT+QGGSG  + ++F+N+RMENV+NPIIIDQ YCD   CE Q 
Sbjct: 308 INVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDKCEDQE 367

Query: 366 SAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLE-----------KTDGT 385
           SAV++  ++Y+NISGT+ +  A+   CS+ +PC  I+L NV ++           K  GT
Sbjct: 368 SAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKNQGT 427

BLAST of Cp4.1LG01g15400 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-75
Identity = 154/370 (41.62%), Postives = 214/370 (57.84%), Query Frame = 0

Query: 66  INVDSFDAVGDGVTDDTMAFRKAWETACSSSNSV-LLVPKEGRYLVNATKFKGPCKHGMV 125
           ++V  F A GDG TDDT AF  AW+ ACSS+ +V LLVPK   YL+ + +  GPC   + 
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 127

Query: 126 IQIEGTIVAPDEPENWDPKLPRQWLEFTKLSAATFQG--DGIIDGSGEKWWAESCKKNKS 185
           +QI GT+ A  +    D K   +W+ F  ++  +  G   G++DG+GE WW  SCK+NK+
Sbjct: 128 VQIFGTLSASQKRS--DYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKA 187

Query: 186 RPCKGAPTALTIESSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTDGI 245
           +PC  APTALT  +S ++ VK L ++N+QQ+   I +   V+VS V V+AP DSPNTDGI
Sbjct: 188 KPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGI 247

Query: 246 HITQSTNVVVENCKIST--------------------------------GKDNSTGIVTK 305
           HIT + N+ V    I T                                G DNS   V+ 
Sbjct: 248 HITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSG 307

Query: 306 VVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDHTTCEPQA 365
           V +D A L  T NGVRIKT+QGGSG    + F+N++M+NV+NPIIIDQ YCD + C  + 
Sbjct: 308 VTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEK 367

Query: 366 SAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAAQGF 401
           SAV++  ++YR+ISGT+ S+NA+ F CS ++PC  I+L  VN++    T    C  A   
Sbjct: 368 SAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKGGKAT----CTNANVV 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AJ51.0e-8643.11Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353366.1e-7945.23Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q020961.1e-7744.20Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
O231472.2e-7641.92Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Q8RY292.6e-7441.62Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KAG6601508.10.099.08putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022977184.14.28e-31192.52probable polygalacturonase At1g80170 [Cucurbita maxima][more]
XP_022956901.11.22e-31092.09probable polygalacturonase At1g80170 [Cucurbita moschata][more]
XP_011655619.16.59e-27482.09probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus][more]
XP_008446196.11.29e-27382.09PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1IQQ82.07e-31192.52probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111477357... [more]
A0A6J1GXR95.93e-31192.09probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114584... [more]
A0A0A0KQ653.19e-27482.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1[more]
A0A1S3BF586.25e-27482.09probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=... [more]
A0A6J1FZ302.40e-26778.89probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114492... [more]
Match NameE-valueIdentityDescription
AT1G23460.13.7e-15662.36Pectin lyase-like superfamily protein [more]
AT1G70500.17.7e-14656.05Pectin lyase-like superfamily protein [more]
AT1G80170.17.3e-8843.11Pectin lyase-like superfamily protein [more]
AT3G57510.11.5e-7741.92Pectin lyase-like superfamily protein [more]
AT2G41850.11.9e-7541.62polygalacturonase abscission zone A. thaliana [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 363..384
e-value: 7500.0
score: 1.0
coord: 220..246
e-value: 140.0
score: 11.3
coord: 247..289
e-value: 120.0
score: 11.5
coord: 297..318
e-value: 3800.0
score: 3.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 54..434
e-value: 1.6E-98
score: 332.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 97..263
e-value: 5.9E-38
score: 130.7
coord: 267..384
e-value: 7.3E-33
score: 114.0
NoneNo IPR availablePANTHERPTHR31375:SF205BNAA08G20470D PROTEINcoord: 258..414
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..262
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 258..414
NoneNo IPR availablePANTHERPTHR31375:SF205BNAA08G20470D PROTEINcoord: 2..262
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 64..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g15400.1Cp4.1LG01g15400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0004650 polygalacturonase activity