Cp4.1LG01g13110 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g13110
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWAT1-related protein
LocationCp4.1LG01: 8444814 .. 8447411 (+)
RNA-Seq ExpressionCp4.1LG01g13110
SyntenyCp4.1LG01g13110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATGGAAGTTAAGAAGCAAATATGGGGTGTGGTTCCATTTTTGGCGATGGTGAGCTTGGAAGGCTGCACCATTGCATTAACCATAATGGCCAAAACAGCCATTACTTATGGAATGACCACTTTTGTATTTGTTGTGTACACAAACGCTGTTGCTTCCATTGTTCTTCTTCCTTATTCCTTTATCTTTCATTACAATGAGAGGTTCCAATCCCATTTTAACTCCTTGGTTTACTTGATTAAGCAACGCCACTCTTAAAGTTCTTAACTTTAATTTGTTGGTTGGTTCTTGCAGGACGGAGTTTCAACAATCTTTGTTCTCCTTTCCTCTTTTCCTCCGAGTCTTTCTCTTGGGTTTAATAGGGTTTGTCCTCTCTCTCGCCTATAATTCACCTATGTTTTGATACCATTATGTATTGAACTCATTATATTGTGTGTAGGATATGTGTATCTCAAAACCTAGCATTTTTGGGACTAAGCTACAGCTCTCCTATTGTTGTGTGCGCAATGGGTCTTATGCTTCCGGCCATTTCCTTCGTACTTTCGATCATTCTCGGGTAATCTTTTGAAATATATATGTGAGATTCTATGTTCGTTGGAGAGGGTAATGAAGCATTCTTTATAAGAGTGTAGAAACCTCTCCTTAATGGATGCGTTTTAAAACCTTGGGAGAAAGCCCAAAAAGAACAATATTTACTGAAGGTGAGTTTGAGCTGTTACAAATGGTATCAGAGCTAGACATTGGGGTAGTGTGCCGGCGAGGATGTTACACATCAGTTGGATTGGGGAACGAAACACTTCTTATGAGGGTGTAGAAACCTCTTTGTACTGGGCTTGAACTGTTACAAATGGTATTAGAGACAGACAACATGCAGTGTGCCAGCTAGGACGTTGGTTCACAAGGAGGGTGGATTGTGAGATCCCACATCGATTGAAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTGGATTGTGAGATCCCACATCGATTAAAGAAGAGAACGAAACATTCTTTCTAAGGGTGTAGAAATCTTTCTCTAACGGATACGTTTTAAAACCTTGAGGGGAAGCCCGAAAAAGAAAGTCTAAAGAGGACAATAAGGCTTGGATTATTACAAATGATATTAGAGCTAGACACTGGGCGGTGTGCCAATGAGGACGCTGGGCCTACAAGGGGGTAGATTGTGAGATCCCACATCGATTGAAGAGTAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCTCTCTCATGTATATGTTTTAAAACCTTGAGGGGAAGCCCGAAAAAGAAAGTCCAAAGAGAACAATAGGGCTTAGACGGTTACAAATGATATTAGAGCTAGACACCGGGCGGTGTGCCAATGAGGACGTTAGGCCTATAAGGGAGGTGGATTGTGAGATCCCACATCGGTTGGAGAACGAAACAAAACATTTCTTGTGTGGAAAGTCTCTCTAACAAACGCGTTTTAAAGTCTTGGATATTGTGATTGTTATTATATGGACTAATATGGTTTGAGATGATTCAGGAGGACAAAAATCAACTGGAAAAGCCCCAGCTTCATTACCAAAGTAGTAGGGACAATGATATCAGTTTTAGGAGCAACATCAGAGGAGCTTTACATAGGCCCAGCAGTGCGTCAACATCCATCCTCCTCTTCACATTTACAATTCAAACACAAGCTTCTAGTCTTCACCTCCACAACAGATCGCTGGATCATGGGTGGCCTCTTACTAGCAGCTGCTTCCTTATGCGTCTCAATATGGAACATCATTCAGGTTCATACGAACAACATTACACAATCCCATGATGTTTTTTTTTGCATTAAACTTGACACCCTTTTGTGTTCTCCTCCTTTTGGTCAGCTGGGAGTAATAAAACAATACCCTCAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCACCTTACTCTCTGCAGCAGTGGGCTTCTTTGTAGTCAACGATTCAAGCGCATGGACAATCAAATCCAGCTTCGACCTCTTTCTCATTATTGCAACAGTAAGAAACACATAATTAACTCAATAGAGATGTTGTTTTAGACTGTTTCTGAATGAAAAAGATGAACTTTGTAGGGAATTTTCAGCAGTTTGATTCGAAACCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCTTTTGGCATTCTTTTTGCTACCTTCTTTGGCGTCACCTTCTTTGGAGACACTTTTCACTATGGAAGGTACTAACTTTTTTCCTCGGCCCAACGCTAGTAAATATTGTAATCTTTTAAACTTTACTTTAAAGCTTTAAAATACGTCTGCTATGGAAAGGTTTCCACATTTTTATAAAGGGTGTTTCATTCTCCTTCCCAACGAATGAATATTATAATTAGATTAACCTTTTAAGCGGGAATGATTGGCAGTGTGATGGCAGCATTTATAGCTGGAATGGGATATTTGACAGTAATGTGGGGGCAAATCATCGAAGATAATGGAGTGGGTGAAGACAAAGATGACAAGAATAATGACCATTTGTCTTCTGCCAAACTTCCACTTCTGGACGAAGAATCAAAAGTG

mRNA sequence

ACATGGAAGTTAAGAAGCAAATATGGGGTGTGGTTCCATTTTTGGCGATGGTGAGCTTGGAAGGCTGCACCATTGCATTAACCATAATGGCCAAAACAGCCATTACTTATGGAATGACCACTTTTGTATTTGTTGTGTACACAAACGCTGTTGCTTCCATTGACGGAGTTTCAACAATCTTTGTTCTCCTTTCCTCTTTTCCTCCGAGTCTTTCTCTTGGGTTTAATAGGGATATGTGTATCTCAAAACCTAGCATTTTTGGGACTAAGCTACAGCTCTCCTATTGTTGTGTGCGCAATGGGAGGACAAAAATCAACTGGAAAAGCCCCAGCTTCATTACCAAAGTAGTAGGGACAATGATATCAGTTTTAGGAGCAACATCAGAGGAGCTTTACATAGGCCCAGCAGTGCGTCAACATCCATCCTCCTCTTCACATTTACAATTCAAACACAAGCTTCTAGTCTTCACCTCCACAACAGATCGCTGGATCATGGGTGGCCTCTTACTAGCAGCTGCTTCCTTATGCGTCTCAATATGGAACATCATTCAGGTTCATACGAACAACATTACACAATCCCATGATGTTTTTTTTTGCATTAAACTTGACACCCTTTTGTGTTCTCCTCCTTTTGGTCAGCTGGGAGTAATAAAACAATACCCTCAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCACCTTACTCTCTGCAGCAGTGGGCTTCTTTGTAGTCAACGATTCAAGCGCATGGACAATCAAATCCAGCTTCGACCTCTTTCTCATTATTGCAACACAGTTTGATTCGAAACCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCTTTTGGCATTCTTTTTGCTACCTTCTTTGGCTAATGTGGGGGCAAATCATCGAAGATAATGGAGTGGGTGAAGACAAAGATGACAAGAATAATGACCATTTGTCTTCTGCCAAACTTCCACTTCTGGACGAAGAATCAAAAGTG

Coding sequence (CDS)

ATGGAAGTTAAGAAGCAAATATGGGGTGTGGTTCCATTTTTGGCGATGGTGAGCTTGGAAGGCTGCACCATTGCATTAACCATAATGGCCAAAACAGCCATTACTTATGGAATGACCACTTTTGTATTTGTTGTGTACACAAACGCTGTTGCTTCCATTGACGGAGTTTCAACAATCTTTGTTCTCCTTTCCTCTTTTCCTCCGAGTCTTTCTCTTGGGTTTAATAGGGATATGTGTATCTCAAAACCTAGCATTTTTGGGACTAAGCTACAGCTCTCCTATTGTTGTGTGCGCAATGGGAGGACAAAAATCAACTGGAAAAGCCCCAGCTTCATTACCAAAGTAGTAGGGACAATGATATCAGTTTTAGGAGCAACATCAGAGGAGCTTTACATAGGCCCAGCAGTGCGTCAACATCCATCCTCCTCTTCACATTTACAATTCAAACACAAGCTTCTAGTCTTCACCTCCACAACAGATCGCTGGATCATGGGTGGCCTCTTACTAGCAGCTGCTTCCTTATGCGTCTCAATATGGAACATCATTCAGGTTCATACGAACAACATTACACAATCCCATGATGTTTTTTTTTGCATTAAACTTGACACCCTTTTGTGTTCTCCTCCTTTTGGTCAGCTGGGAGTAATAAAACAATACCCTCAAGTCATGAAAGTTGCCTCCTTTTATAGTTTGGTGGGCACCTTACTCTCTGCAGCAGTGGGCTTCTTTGTAGTCAACGATTCAAGCGCATGGACAATCAAATCCAGCTTCGACCTCTTTCTCATTATTGCAACACAGTTTGATTCGAAACCGAGTGCAAATATGGTGCATGCAAATGAAAGGGCCATATTACGTGCCCATGTTCAAGCCTTTTGGCATTCTTTTTGCTACCTTCTTTGGCTAATGTGGGGGCAAATCATCGAAGATAATGGAGTGGGTGAAGACAAAGATGACAAGAATAATGACCATTTGTCTTCTGCCAAACTTCCACTTCTGGACGAAGAATCAAAAGTG

Protein sequence

MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQLSYCCVRNGRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQFDSKPSANMVHANERAILRAHVQAFWHSFCYLLWLMWGQIIEDNGVGEDKDDKNNDHLSSAKLPLLDEESKV
Homology
BLAST of Cp4.1LG01g13110 vs. ExPASy Swiss-Prot
Match: F4I5D5 (WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.2e-35
Identity = 109/294 (37.07%), Postives = 154/294 (52.38%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVK +   +VPF+AM  +E CTIALTIMAKTA+T GM+ FVFVVYTNA  SI  +   F
Sbjct: 1   MEVKVRRDELVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSF 60

Query: 61  VL---------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQ--LSYCC---------- 120
           +          + S+P  +    LGF         +  G +    +  C           
Sbjct: 61  LFHRNERTEQSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSF 120

Query: 121 ---VRNGRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSH--LQFKHK 180
              +  GR+K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   K
Sbjct: 121 LLSIILGRSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPK 180

Query: 181 LLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFG 240
           LLV+ +  D W +G + LA A   VS++N++Q                            
Sbjct: 181 LLVYYNLPDNWFLGCIFLAVAVFSVSLFNVVQT--------------------------- 240

Query: 241 QLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT 266
             G +K+YP VMKVASFYS+VGT+       F+  D SAW I+ +FDL+LIIAT
Sbjct: 241 --GTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERDLSAWKIQPNFDLYLIIAT 265

BLAST of Cp4.1LG01g13110 vs. ExPASy Swiss-Prot
Match: Q9ZUI8 (WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.8e-31
Identity = 101/289 (34.95%), Postives = 134/289 (46.37%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI------------DGVS 69
           +VPF+ M  +E CTIALTI+AKTA+T GM+ FVF+VYTNA+ S+            +   
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 70  TIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQ------------------LSYCCVRN 129
             F+   S      LGF         +  G                      LS    + 
Sbjct: 71  EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALGKE 130

Query: 130 GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKL---LVFT 189
           G   + W S     +V+GT+I   GA  E +Y+GP +R  P SS    F   +   L F 
Sbjct: 131 G--GLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 190 STTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVI 249
             +D W +G LLLA A+L +SIWNIIQ+ T                             +
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDT-----------------------------V 250

Query: 250 KQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT 266
           ++YPQVMKV S YSL GTL  A    F+  D SAW +K + DL+LIIAT
Sbjct: 251 QKYPQVMKVVSAYSLAGTLQCAIFSAFMEPDLSAWELKLNMDLYLIIAT 268

BLAST of Cp4.1LG01g13110 vs. ExPASy Swiss-Prot
Match: F4JK59 (WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.9e-12
Identity = 80/305 (26.23%), Postives = 124/305 (40.66%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVLLSSFPPS 69
           VVPF AM+++E  T+  +I+ K A   G + +VFV Y        G + + +LL     S
Sbjct: 15  VVPFTAMIAIECTTVGSSILYKAATLRGFSFYVFVFYAYV-----GATLVLLLL-----S 74

Query: 70  LSLGFNRDMCISKPSIFGTKLQLSYCCVRN---GRTKINWKSP-------------SFI- 129
           L  G +R +  +K S+F     L+   + +   G   I + SP             +FI 
Sbjct: 75  LIFGRSRSLPTAKSSLFFKIFLLALLGLTSRVAGCKGIEYSSPTLSSAISNLTPAFTFIL 134

Query: 130 -----------------TKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLV- 189
                             K++GT++S+ GA    LY GP                KLLV 
Sbjct: 135 AIFFRMEQVMLRSSATQAKIIGTIVSISGALVIVLYKGP----------------KLLVA 194

Query: 190 --FTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQ 249
             FTS    WI+GGLLL    L +S+W I+Q H                           
Sbjct: 195 ASFTSFESSWIIGGLLLGLQFLLLSVWFILQTH--------------------------- 254

Query: 250 LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQFDSKPS 278
             +++ YP+ + V   Y+L  TL+S  V   V  D ++W +K  F L  +I +       
Sbjct: 255 --IMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLKPGFSLASVIYSGLFDTSL 264

BLAST of Cp4.1LG01g13110 vs. ExPASy Swiss-Prot
Match: F4KHA8 (WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 78/288 (27.08%), Postives = 119/288 (41.32%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVLLSSFPPS 69
           VVPF AMV++E  T+    + K A   G++ +VFV YT  VA++       VLL   P S
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATL-------VLL---PLS 78

Query: 70  LSLGFNRDMCISKPSIFGTKLQLS---YCCVRNGRTKINWKSPSFIT------------- 129
           L  G ++ +  +K  +F     L+   +  +  G   I + SP+  +             
Sbjct: 79  LIFGRSKRLPSAKTPVFFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLTPAFTFTL 138

Query: 130 ------------------KVVGTMISVLGATSEELYIGPAVRQH----PSSSSHLQFKHK 189
                             K++GT++S+ GA    LY GP V       P S +   ++H 
Sbjct: 139 AVIFRMEQIVLRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQH- 198

Query: 190 LLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFG 249
               TS    WI+GGLLLA   L VS+W I+Q                            
Sbjct: 199 ---LTSFDSSWIIGGLLLATQYLLVSVWYILQTR-------------------------- 258

Query: 250 QLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDL 260
              V++ YP+ + V   Y+L  TL+SA V  F   D +++ +K    L
Sbjct: 259 ---VMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSL 263

BLAST of Cp4.1LG01g13110 vs. ExPASy Swiss-Prot
Match: Q56X95 (WAT1-related protein At3g28130 OS=Arabidopsis thaliana OX=3702 GN=At3g28130 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.6e-10
Identity = 71/273 (26.01%), Postives = 118/273 (43.22%), Query Frame = 0

Query: 15  AMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGV-STIFVLLSSFPPSLSLG 74
           AM++ E   +A+  + K A + G+ ++ F++Y+  + SI  + S IF   S   PSLSL 
Sbjct: 16  AMLATETGNVAMNTLFKAATSKGLNSYTFLIYSYLIGSIVLLPSHIFSYRSRSLPSLSLS 75

Query: 75  FNRDMCI------------------SKPSIFGTKLQLS-----YCCVRNGRTKINWKSPS 134
               + +                  S P++      ++        +     K ++K  S
Sbjct: 76  ILCKIGVLGLLGSTYLITGFIGIEYSNPTLASAISNINPAITFILAIIFRMEKASFKEKS 135

Query: 135 FITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLA 194
            + K+VGT++S++GA    LY GP V   PSS    Q +  LL  +S+   WI+GG LLA
Sbjct: 136 SVAKMVGTIVSLVGALVVVLYHGPRVFT-PSSPPFPQLRQLLLPLSSSNSDWIIGGCLLA 195

Query: 195 AASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVIKQYPQVMKVASFYS 254
                V +  I+Q H                             ++K YP    V+ FY 
Sbjct: 196 IKDTLVPVAFILQAH-----------------------------IMKLYPAPFTVSFFYF 255

Query: 255 LVGTLLSAAVGFFV-VNDSSAWTIKSSFDLFLI 263
           L+ ++L++ +G     N+ S W I   FD+ L+
Sbjct: 256 LIASILTSLIGIVAEKNNPSIWII--HFDITLV 256

BLAST of Cp4.1LG01g13110 vs. NCBI nr
Match: KAG7032149.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466 bits (1199), Expect = 2.96e-161
Identity = 281/424 (66.27%), Postives = 285/424 (67.22%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI------- 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI       
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSF 60

Query: 61  ---------------DGVSTIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQLSYCCVR 120
                          DGVSTIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQLSYCCVR
Sbjct: 61  IFHYNERFQSHFNSLDGVSTIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQLSYCCVR 120

Query: 121 NG-----------------------------------RTKINWKSPSFITKVVGTMISVL 180
           NG                                   RTKINWKSPSFITKVVGTMISV+
Sbjct: 121 NGSYASGHFLPTFDHSRKSLCSGLKLLQMVLETDNMRRTKINWKSPSFITKVVGTMISVI 180

Query: 181 GATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ 240
           GATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNI+Q
Sbjct: 181 GATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQ 240

Query: 241 VHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFF 300
                                        LGVIKQYPQVMKVASFYSLVGTLLSAAVGFF
Sbjct: 241 -----------------------------LGVIKQYPQVMKVASFYSLVGTLLSAAVGFF 300

Query: 301 VVNDSSAWTIKSSFDLFLIIATQFDSKPSANMVHA--------------NERAILRAH-- 338
           VVNDSSAWTIKSSFDLFLIIAT   S    N V                    IL A   
Sbjct: 301 VVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 360

BLAST of Cp4.1LG01g13110 vs. NCBI nr
Match: XP_023531064.1 (WAT1-related protein At1g70260-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393 bits (1009), Expect = 8.03e-133
Identity = 254/400 (63.50%), Postives = 261/400 (65.25%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +   F
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSF 60

Query: 61  VL-----------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           +            L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFVLSIILGRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. NCBI nr
Match: KAG6601366.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389 bits (998), Expect = 3.75e-131
Identity = 251/400 (62.75%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +   F
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSF 60

Query: 61  VL-----------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           +            L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISV+GATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFLLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNI+Q                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. NCBI nr
Match: XP_022957168.1 (WAT1-related protein At1g70260-like [Cucurbita moschata])

HSP 1 Score: 389 bits (998), Expect = 3.75e-131
Identity = 251/400 (62.75%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +   F
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSF 60

Query: 61  VL-----------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           +            L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISV+GATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNI+Q                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. NCBI nr
Match: XP_022975833.1 (WAT1-related protein At1g70260-like [Cucurbita maxima])

HSP 1 Score: 387 bits (994), Expect = 1.52e-130
Identity = 254/400 (63.50%), Postives = 261/400 (65.25%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGV--ST 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +  S 
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSL 60

Query: 61  IFVL---------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           IF           L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISV+GATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFVLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATLFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. ExPASy TrEMBL
Match: A0A6J1GZG2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 1.81e-131
Identity = 251/400 (62.75%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +   F
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSF 60

Query: 61  VL-----------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           +            L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISV+GATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNI+Q                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. ExPASy TrEMBL
Match: A0A6J1IKD8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1)

HSP 1 Score: 387 bits (994), Expect = 7.34e-131
Identity = 254/400 (63.50%), Postives = 261/400 (65.25%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGV--ST 60
           MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI  +  S 
Sbjct: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYSL 60

Query: 61  IFVL---------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-- 120
           IF           L SFP  L    LG    +C+S+   F   L LSY      C     
Sbjct: 61  IFHYNERTEFQQSLFSFPLFLRVFLLGL-IGICVSQNLAF---LGLSYSSPIVVCAMGLM 120

Query: 121 ------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQ 180
                       GRTKINWKSPSFITKVVGTMISV+GATSEELYIGPAVRQHPSSSSHLQ
Sbjct: 121 LPAISFVLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQ 180

Query: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCS 240
           FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ                        
Sbjct: 181 FKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQ------------------------ 240

Query: 241 PPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQF 300
                LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT  
Sbjct: 241 -----LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATGI 300

Query: 301 DSKPSANMVHA--------------NERAILRAH---VQAFWHSFCY------------L 338
            S    N V                    IL A    V  F  +F Y             
Sbjct: 301 FSSLIRNRVQIWCMQMKGPYYVPMFKPFGILFATLFGVTFFGDTFHYGSVMAAFIAGMGY 360

BLAST of Cp4.1LG01g13110 vs. ExPASy TrEMBL
Match: A0A1S3BEK3 (WAT1-related protein At1g70260 OS=Cucumis melo OX=3656 GN=LOC103489028 PE=4 SV=1)

HSP 1 Score: 303 bits (777), Expect = 6.80e-98
Identity = 207/402 (51.49%), Postives = 238/402 (59.20%), Query Frame = 0

Query: 1   MEVK--KQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVST 60
           MEVK  + +  VVPFLAMV++EGCTIALTI+AKTAITYGM+TFVFVVYTNAVAS+  +  
Sbjct: 3   MEVKGGRILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPY 62

Query: 61  IFVL-----------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN 120
            F+            L SFP  L    LG    +CIS+   F   L LSY      C   
Sbjct: 63  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLT-GICISQNLAF---LGLSYSSPIVVCAMG 122

Query: 121 --------------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSH 180
                         G+TKI W++P+FITKVVGT+ISV+GAT EELY+GP VRQHPSSS+H
Sbjct: 123 LMLPSISFLLSILLGKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHPSSSTH 182

Query: 181 LQFKHKLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLL 240
           LQFK KLLVFTSTTDRWI GGLLLAAA+LCVSIWNIIQ                      
Sbjct: 183 LQFKQKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQ---------------------- 242

Query: 241 CSPPFGQLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT 300
                  LGV+KQY QVMKV SFYS+VGT +SA V +FVVNDSSAWTIKSSFDL+LIIAT
Sbjct: 243 -------LGVVKQYSQVMKVGSFYSIVGTFMSAIVAYFVVNDSSAWTIKSSFDLYLIIAT 302

Query: 301 QFDSKPSANMVHANERAILRAHVQAFWHSFCYL--------------------------- 335
              S    N V          H    +  F  L                           
Sbjct: 303 GTFSGLIRNRVQIWCMQKKGPHYVPMFKPFGILFATFFGATFFGDTFHYGSVMAAFIAGM 362

BLAST of Cp4.1LG01g13110 vs. ExPASy TrEMBL
Match: A0A6J1FLY1 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447017 PE=3 SV=1)

HSP 1 Score: 303 bits (776), Expect = 7.01e-98
Identity = 209/395 (52.91%), Postives = 237/395 (60.00%), Query Frame = 0

Query: 7   IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVAS--IDGVSTIFVL-- 66
           +WGVVPFLAMVSLEGCTI LTI+AKTAITYG  TFVFVVYTNAVAS  +   S +F L  
Sbjct: 9   LWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFHLND 68

Query: 67  -------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN-------- 126
                  L S P  L    LGF   +C+S+   F   L LSY      CV          
Sbjct: 69  RTDYQQSLFSLPLLLRIFLLGF-IGICVSQNLAF---LGLSYSSPIVVCVMGLMLPAISF 128

Query: 127 ------GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLL 186
                  RTKINWKSPSFITKVVGT+ISV+GATSEELY+GP VRQHPSSS HLQFK KLL
Sbjct: 129 ILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLL 188

Query: 187 VFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQL 246
           VFTSTTDRWIMGGLLLAAA+LCVSIWNIIQ                             L
Sbjct: 189 VFTSTTDRWIMGGLLLAAATLCVSIWNIIQ-----------------------------L 248

Query: 247 GVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQFDSKPSA 306
           GV+KQY QVMKVASFYSLVG+L+SAA+ FF+V+D +AW IKS FD+FLIIAT   S    
Sbjct: 249 GVVKQYSQVMKVASFYSLVGSLMSAAMAFFIVDDLNAWIIKSKFDIFLIIATGVFSGLIR 308

Query: 307 NMVHA--------------NERAILRAH----------------VQAFWHSFCYLLWLMW 338
           N V                    IL A                 + AF     YL  +MW
Sbjct: 309 NRVQIWCMQMKGPYYVPMFKPFGILFATFFGSTFFGDTFHYGSVMAAFIAGMGYLT-VMW 365

BLAST of Cp4.1LG01g13110 vs. ExPASy TrEMBL
Match: A0A5D3CUM5 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00810 PE=4 SV=1)

HSP 1 Score: 302 bits (774), Expect = 1.32e-97
Identity = 203/391 (51.92%), Postives = 232/391 (59.34%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVL------- 69
           VVPFLAMV++EGCTIALTI+AKTAITYGM+TFVFVVYTNAVAS+  +   F+        
Sbjct: 2   VVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASVILLPYSFIFHYNQRLE 61

Query: 70  ----LSSFPPSLS---LGFNRDMCISKPSIFGTKLQLSYC-----CVRN----------- 129
               L SFP  L    LG    +CIS+   F   L LSY      C              
Sbjct: 62  FQQSLFSFPLLLRVFLLGLT-GICISQNLAF---LGLSYSSPIVVCAMGLMLPSISFLLS 121

Query: 130 ---GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFT 189
              G+TKI W++P+FITKVVGT+ISV+GAT EELY+GP VRQHPSSS+HLQFK KLLVFT
Sbjct: 122 ILLGKTKIEWENPNFITKVVGTVISVIGATCEELYLGPTVRQHPSSSTHLQFKQKLLVFT 181

Query: 190 STTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVI 249
           STTDRWI GGLLLAAA+LCVSIWNIIQ                             LGV+
Sbjct: 182 STTDRWIFGGLLLAAATLCVSIWNIIQ-----------------------------LGVV 241

Query: 250 KQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQFDSKPSANMV 309
           KQY QVMKV SFYS+VGT +SA V +FVVNDSSAWTIKSSFDL+LIIAT   S    N V
Sbjct: 242 KQYSQVMKVGSFYSIVGTFMSAIVAYFVVNDSSAWTIKSSFDLYLIIATGTFSGLIRNRV 301

Query: 310 HANERAILRAHVQAFWHSFCYL-----------------------------LWLMWGQII 335
                     H    +  F  L                             L +MWGQ+ 
Sbjct: 302 QIWCMQKKGPHYVPMFKPFGILFATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQVN 359

BLAST of Cp4.1LG01g13110 vs. TAIR 10
Match: AT1G70260.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 150.6 bits (379), Expect = 2.3e-36
Identity = 109/294 (37.07%), Postives = 154/294 (52.38%), Query Frame = 0

Query: 1   MEVKKQIWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIF 60
           MEVK +   +VPF+AM  +E CTIALTIMAKTA+T GM+ FVFVVYTNA  SI  +   F
Sbjct: 1   MEVKVRRDELVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSF 60

Query: 61  VL---------LSSFPPSLS---LGFNRDMCISKPSIFGTKLQ--LSYCC---------- 120
           +          + S+P  +    LGF         +  G +    +  C           
Sbjct: 61  LFHRNERTEQSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSF 120

Query: 121 ---VRNGRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSH--LQFKHK 180
              +  GR+K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   K
Sbjct: 121 LLSIILGRSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPK 180

Query: 181 LLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFG 240
           LLV+ +  D W +G + LA A   VS++N++Q                            
Sbjct: 181 LLVYYNLPDNWFLGCIFLAVAVFSVSLFNVVQT--------------------------- 240

Query: 241 QLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT 266
             G +K+YP VMKVASFYS+VGT+       F+  D SAW I+ +FDL+LIIAT
Sbjct: 241 --GTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERDLSAWKIQPNFDLYLIIAT 265

BLAST of Cp4.1LG01g13110 vs. TAIR 10
Match: AT1G60050.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 136.7 bits (343), Expect = 3.4e-32
Identity = 101/289 (34.95%), Postives = 134/289 (46.37%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASI------------DGVS 69
           +VPF+ M  +E CTIALTI+AKTA+T GM+ FVF+VYTNA+ S+            +   
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 70  TIFVLLSSFPPSLSLGFNRDMCISKPSIFGTKLQ------------------LSYCCVRN 129
             F+   S      LGF         +  G                      LS    + 
Sbjct: 71  EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALGKE 130

Query: 130 GRTKINWKSPSFITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKL---LVFT 189
           G   + W S     +V+GT+I   GA  E +Y+GP +R  P SS    F   +   L F 
Sbjct: 131 G--GLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 190 STTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVI 249
             +D W +G LLLA A+L +SIWNIIQ+ T                             +
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDT-----------------------------V 250

Query: 250 KQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIAT 266
           ++YPQVMKV S YSL GTL  A    F+  D SAW +K + DL+LIIAT
Sbjct: 251 QKYPQVMKVVSAYSLAGTLQCAIFSAFMEPDLSAWELKLNMDLYLIIAT 268

BLAST of Cp4.1LG01g13110 vs. TAIR 10
Match: AT4G15540.1 (EamA-like transporter family )

HSP 1 Score: 74.3 bits (181), Expect = 2.1e-13
Identity = 80/305 (26.23%), Postives = 124/305 (40.66%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVLLSSFPPS 69
           VVPF AM+++E  T+  +I+ K A   G + +VFV Y        G + + +LL     S
Sbjct: 15  VVPFTAMIAIECTTVGSSILYKAATLRGFSFYVFVFYAYV-----GATLVLLLL-----S 74

Query: 70  LSLGFNRDMCISKPSIFGTKLQLSYCCVRN---GRTKINWKSP-------------SFI- 129
           L  G +R +  +K S+F     L+   + +   G   I + SP             +FI 
Sbjct: 75  LIFGRSRSLPTAKSSLFFKIFLLALLGLTSRVAGCKGIEYSSPTLSSAISNLTPAFTFIL 134

Query: 130 -----------------TKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLV- 189
                             K++GT++S+ GA    LY GP                KLLV 
Sbjct: 135 AIFFRMEQVMLRSSATQAKIIGTIVSISGALVIVLYKGP----------------KLLVA 194

Query: 190 --FTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQ 249
             FTS    WI+GGLLL    L +S+W I+Q H                           
Sbjct: 195 ASFTSFESSWIIGGLLLGLQFLLLSVWFILQTH--------------------------- 254

Query: 250 LGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDLFLIIATQFDSKPS 278
             +++ YP+ + V   Y+L  TL+S  V   V  D ++W +K  F L  +I +       
Sbjct: 255 --IMEIYPEEIAVVFCYNLCATLISGTVCLLVEKDLNSWQLKPGFSLASVIYSGLFDTSL 264

BLAST of Cp4.1LG01g13110 vs. TAIR 10
Match: AT5G40230.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-12
Identity = 78/288 (27.08%), Postives = 119/288 (41.32%), Query Frame = 0

Query: 10  VVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGVSTIFVLLSSFPPS 69
           VVPF AMV++E  T+    + K A   G++ +VFV YT  VA++       VLL   P S
Sbjct: 19  VVPFTAMVAVECVTVGSNTLFKAATLRGLSFYVFVFYTYVVATL-------VLL---PLS 78

Query: 70  LSLGFNRDMCISKPSIFGTKLQLS---YCCVRNGRTKINWKSPSFIT------------- 129
           L  G ++ +  +K  +F     L+   +  +  G   I + SP+  +             
Sbjct: 79  LIFGRSKRLPSAKTPVFFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLTPAFTFTL 138

Query: 130 ------------------KVVGTMISVLGATSEELYIGPAVRQH----PSSSSHLQFKHK 189
                             K++GT++S+ GA    LY GP V       P S +   ++H 
Sbjct: 139 AVIFRMEQIVLRSSATQAKIIGTIVSISGALVVILYKGPKVLTDASLTPPSPTISLYQH- 198

Query: 190 LLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFG 249
               TS    WI+GGLLLA   L VS+W I+Q                            
Sbjct: 199 ---LTSFDSSWIIGGLLLATQYLLVSVWYILQTR-------------------------- 258

Query: 250 QLGVIKQYPQVMKVASFYSLVGTLLSAAVGFFVVNDSSAWTIKSSFDL 260
              V++ YP+ + V   Y+L  TL+SA V  F   D +++ +K    L
Sbjct: 259 ---VMELYPEEITVVFLYNLCATLISAPVCLFAEKDLNSFILKPGVSL 263

BLAST of Cp4.1LG01g13110 vs. TAIR 10
Match: AT3G28130.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.3e-11
Identity = 71/273 (26.01%), Postives = 118/273 (43.22%), Query Frame = 0

Query: 15  AMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIDGV-STIFVLLSSFPPSLSLG 74
           AM++ E   +A+  + K A + G+ ++ F++Y+  + SI  + S IF   S   PSLSL 
Sbjct: 16  AMLATETGNVAMNTLFKAATSKGLNSYTFLIYSYLIGSIVLLPSHIFSYRSRSLPSLSLS 75

Query: 75  FNRDMCI------------------SKPSIFGTKLQLS-----YCCVRNGRTKINWKSPS 134
               + +                  S P++      ++        +     K ++K  S
Sbjct: 76  ILCKIGVLGLLGSTYLITGFIGIEYSNPTLASAISNINPAITFILAIIFRMEKASFKEKS 135

Query: 135 FITKVVGTMISVLGATSEELYIGPAVRQHPSSSSHLQFKHKLLVFTSTTDRWIMGGLLLA 194
            + K+VGT++S++GA    LY GP V   PSS    Q +  LL  +S+   WI+GG LLA
Sbjct: 136 SVAKMVGTIVSLVGALVVVLYHGPRVFT-PSSPPFPQLRQLLLPLSSSNSDWIIGGCLLA 195

Query: 195 AASLCVSIWNIIQVHTNNITQSHDVFFCIKLDTLLCSPPFGQLGVIKQYPQVMKVASFYS 254
                V +  I+Q H                             ++K YP    V+ FY 
Sbjct: 196 IKDTLVPVAFILQAH-----------------------------IMKLYPAPFTVSFFYF 255

Query: 255 LVGTLLSAAVGFFV-VNDSSAWTIKSSFDLFLI 263
           L+ ++L++ +G     N+ S W I   FD+ L+
Sbjct: 256 LIASILTSLIGIVAEKNNPSIWII--HFDITLV 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I5D53.2e-3537.07WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2... [more]
Q9ZUI84.8e-3134.95WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3... [more]
F4JK592.9e-1226.23WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2... [more]
F4KHA82.5e-1127.08WAT1-related protein At5g40230 OS=Arabidopsis thaliana OX=3702 GN=At5g40230 PE=3... [more]
Q56X954.6e-1026.01WAT1-related protein At3g28130 OS=Arabidopsis thaliana OX=3702 GN=At3g28130 PE=2... [more]
Match NameE-valueIdentityDescription
KAG7032149.12.96e-16166.27WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023531064.18.03e-13363.50WAT1-related protein At1g70260-like [Cucurbita pepo subsp. pepo][more]
KAG6601366.13.75e-13162.75WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022957168.13.75e-13162.75WAT1-related protein At1g70260-like [Cucurbita moschata][more]
XP_022975833.11.52e-13063.50WAT1-related protein At1g70260-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GZG21.81e-13162.75WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1[more]
A0A6J1IKD87.34e-13163.50WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1[more]
A0A1S3BEK36.80e-9851.49WAT1-related protein At1g70260 OS=Cucumis melo OX=3656 GN=LOC103489028 PE=4 SV=1[more]
A0A6J1FLY17.01e-9852.91WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447017 PE=3 SV=1[more]
A0A5D3CUM51.32e-9751.92WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G... [more]
Match NameE-valueIdentityDescription
AT1G70260.12.3e-3637.07nodulin MtN21 /EamA-like transporter family protein [more]
AT1G60050.13.4e-3234.95Nodulin MtN21 /EamA-like transporter family protein [more]
AT4G15540.12.1e-1326.23EamA-like transporter family [more]
AT5G40230.11.7e-1227.08nodulin MtN21 /EamA-like transporter family protein [more]
AT3G28130.23.3e-1126.01nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 317..338
NoneNo IPR availablePANTHERPTHR31218:SF61WAT1-RELATED PROTEINcoord: 9..55
coord: 100..268
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..55
coord: 100..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g13110.1Cp4.1LG01g13110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity