Homology
BLAST of Cp4.1LG01g06240 vs. ExPASy Swiss-Prot
Match:
Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)
HSP 1 Score: 1544.3 bits (3997), Expect = 0.0e+00
Identity = 902/1778 (50.73%), Postives = 1128/1778 (63.44%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCL----SEEFVAPNKSYKMCCECDMSFSKTSLV 60
MGIPD SL DLI+KVRSWI+ S+ L ++F KMC +C +K
Sbjct: 1 MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60
Query: 61 YLCQSCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEK 120
Y C SC C++C ES + K C+ C D +R K
Sbjct: 61 YCCLSCGSCWCKSCSDTEESKM----------------KLCREC-DAEVR-----ELRVK 120
Query: 121 VYSSVSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLF 180
Y V PR SP+ P S TES + L LE +DC
Sbjct: 121 SYDKVHPRDSPDPP---------SSLATES---ESLASSLEIRDC--------------- 180
Query: 181 NDHPSPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEE 240
+ S+ P+ +E++ G+ SPSSD ++DSSDIES S+S R+E FS
Sbjct: 181 ---RNMASIRCYPSRGEEEEARYCGKQLLSPSSDNYQDSSDIESGSVSARHELFSCKSSA 240
Query: 241 -SSPSASPSRSDYT--SNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPS 300
SSP SP R++++ H P + E +L +D+ P VL
Sbjct: 241 GSSPHDSPLRNNFSPLGRFVQHAKDLRSP--TVCSFDNHQEQLL--ADNLVKPGQGVLEQ 300
Query: 301 TQNQDE--NAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASR 360
+++E + LDFENN RIW+PPPP+DE+D+ E+N+F YDDEDDDIGDS FS S
Sbjct: 301 EDHEEEEDKLQQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSS 360
Query: 361 SLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGD-SVEDWVDVITTIA 420
S SS P K+ L E + EPLR +V HFRALVA+LL+GE++ D S +W+D++T +A
Sbjct: 361 SFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALA 420
Query: 421 WQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNA 480
WQAANFV+PDT GGSMDPG+Y+K+KCVASG+ ES LI+G+VC+KNI HKRM SQ+KN
Sbjct: 421 WQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNP 480
Query: 481 RLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEY 540
R++LL GSLEY V+ LASFNTLL QEN+H+K II+KIESLRPNVLLVEKS SS A++Y
Sbjct: 481 RVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQY 540
Query: 541 LLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQ 600
LL KEISLVLNVKR LL+RIARCTGA L PS+D +S +RLGHCELFR ERV E++ NQ
Sbjct: 541 LLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQ 600
Query: 601 -NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDE 660
N+K S+TLM+FEGCP+RLGCTV+L+G CREELKKVKHV+QYAVFAAYHLSLETSFL DE
Sbjct: 601 SNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADE 660
Query: 661 GASLPKMP-KRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHH 720
GASLPK+ K+ V S D +S T + +A E+ PM H
Sbjct: 661 GASLPKIRLKQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPEH 720
Query: 721 LDNNNF--------LLSTGFEEEPSIVDTFPGIFNDH----SPSDVGLN----PSLSHYE 780
+ + E E DT G F ++ S S LN P+L
Sbjct: 721 EVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSSNQLNDLHEPTLCLSS 780
Query: 781 ELKDGETVPFDVRSLSESELAETLERRREKSGGIT---EFQKSESFDENDLSSECFSAAD 840
E+ + T + E R E+ + + + ESF E+D+SSE FSAAD
Sbjct: 781 EIPETPT---------QQPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDVSSEYFSAAD 840
Query: 841 THQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESA 900
+HQSILVSFSS CVLK +VCERSRLLRIKFYG FDKPLG++L DDLF +TSSCRSCKE
Sbjct: 841 SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900
Query: 901 EAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSD 960
+AHV CY+HQ GNLTINVRRLPS+KLPGE+DG+IWMWHRCL+CA +DGVPPAT RV+MSD
Sbjct: 901 DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960
Query: 961 AAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTL 1020
AAWGLSFGKFLELSFSNHATANRVA+CGHSLQRDCLRFYGFG+MVAFFRYS I+IL+V L
Sbjct: 961 AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020
Query: 1021 PPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQS 1080
PPS+LEFNSH QQEWI EA+E++GKM ++Y EISD+++RME+KS L E S++ ++ S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080
Query: 1081 HVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYS 1140
++ L + + KE+ +Y L+P + E +L S+DILELNRLRR+L+I H WDH+LY
Sbjct: 1081 RIIGLIDQLVKEKDEYDDALQP-IFEENLQIQGSLDILELNRLRRALMIGAHAWDHQLYL 1140
Query: 1141 LDSLLKKISNPKADVSSPLKRVLKRDSAQSDDKIEFGCNENIHESCLVHKSAGNDLQSMQ 1200
L+S LKK S K + + D + D +++ G +E
Sbjct: 1141 LNSQLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDER------------------- 1200
Query: 1201 KEEPTAVECSKSTLHQVCREEEHTDEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSV 1260
E+ HTD E E IPS ++LSERID AW GS Q++
Sbjct: 1201 ------------------DEQSHTDSEANGDNKDPENIPSPGTSLSERIDSAWLGSFQNL 1260
Query: 1261 TKPQAVSTLQAQEPPDGATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPS 1320
K + ++ + N LRR L P+RV SFDSAIR QERI+KGL PS
Sbjct: 1261 EKAETIAETEGFSAV-----------NSSLRR-LARPIRVQSFDSAIRFQERIQKGLPPS 1320
Query: 1321 TFQLSTIRSFHASADYNSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFI-SASHVP 1380
+ LST+RSFHAS +Y +M+RDPVS++ R +SQ+LP E QKL+L++ S P++I SAS +
Sbjct: 1321 SLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLIVGSAPTYISSASQMA 1380
Query: 1381 EGVRLLLPQTGNIDAVIAVYDNEPTSIIAYALNSREYDDWVANK-FTDNEGGRNVPRIIE 1440
+G R+L+PQ G D V+ VYD++P S+++YA+NS+EY +W+ NK + N+
Sbjct: 1381 DGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEYKEWIVNKGLASSSSSSNLNN--R 1440
Query: 1441 DSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDPKKSPHLRISFTDESSTSG- 1500
+S PS S W+S ++++DYI + YGS D +KSPHL ISF+D +S+S
Sbjct: 1441 ESEPSAFSTWRSL-SMDVDYIQHAVYGSS---------QDDRKSPHLTISFSDRASSSST 1500
Query: 1501 ---GKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS----------- 1560
GKV FSVTCYFA QFD LRK CCP VDFVRSLSRC++WSAQGGKS
Sbjct: 1501 ATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDER 1560
Query: 1561 -----------------------------------------------------GKEVKMD 1620
GKE KMD
Sbjct: 1561 FIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMD 1620
Query: 1621 LMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKVLLDQNLLEALRTKPIFLGSKAKR 1678
LMVMENLF+ R IS++YDLKGS+RSRYNP+T+GADKVLLD NLLE LRT+PIFLGSKAKR
Sbjct: 1621 LMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKR 1648
BLAST of Cp4.1LG01g06240 vs. ExPASy Swiss-Prot
Match:
Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)
HSP 1 Score: 1048.5 bits (2710), Expect = 8.0e-305
Identity = 690/1828 (37.75%), Postives = 995/1828 (54.43%), Query Frame = 0
Query: 1 MGIPDS---SLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVY 60
MG DS + S+++ ++SW+ W + + +S +F P++S ++C ECD F+ + +
Sbjct: 1 MGTRDSNNRTFSEIVGLIKSWLPWRSEPAT-VSRDFWMPDQSCRVCYECDCQFTLINRRH 60
Query: 61 LCQSCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKV 120
C+ C + C C S ++ E + + C +C + +GG S
Sbjct: 61 HCRHCGRVFCGKCTA--NSIPFAPSDLRTPREDWERIRVCNYCFRQWEQGDGGPHVSNIT 120
Query: 121 YSSVSPRYSPEAPSPSYRGTYKSPKKTESIP------------DDRLCRYLESQDCGYSP 180
S SP + S + S S+P D + S + +
Sbjct: 121 ELSTSPSETSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTK 180
Query: 181 YAASNGLLSLFNDHPSPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSIST 240
S F + N DD D +Y +DIE+S
Sbjct: 181 QGKETSRRSSFIATDVEDPSRFALNSIRSDDEYD----------EYGAYQTDIETSHSPR 240
Query: 241 RNEFFSSHFEESSPSASPSRSDYTSNKADHVVQSVDP--GQRLNLLPQEAETILNRSDDH 300
NE++ AD S P Q L L +E + +H
Sbjct: 241 ANEYYGPMEYNGMGIDDVPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEH 300
Query: 301 --KHPFSSVLPSTQNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIG 360
+ + P+ + D+ E +DFENN +W PP P++E DE E+ F DE+D+ G
Sbjct: 301 DGRDECEASSPADISDDQVVEP-VDFENNGLLWVPPEPENEEDERESALF---DEEDNEG 360
Query: 361 DSGAMFSASRSLSSM----FPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK-SGG 420
D+ + R +S + + EE+K+ ++ +V GHFRAL+AQLLQ E+I S
Sbjct: 361 DASGEWGYLRPSTSFGSGEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDE 420
Query: 421 DSVEDWVDVITTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTK 480
+ E W+++IT+++W+AAN ++PD S+ G MDPG Y+KVKC+ASG +S ++KGVVC K
Sbjct: 421 EGKESWLEIITSLSWEAANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKK 480
Query: 481 NIKHKRMTSQFKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNV 540
N+ ++RM+++ + ARLL+LGG LEY VS+ L+SF+TLL QE DHLK ++KI + RPN+
Sbjct: 481 NVVNRRMSTKIEKARLLILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNI 540
Query: 541 LLVEKSVSSRAKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELF 600
LLVEKSVS A+EYLLAK+ISLVLN+KRPLL+RIARCTGA + PS+DH+S +LG+CE F
Sbjct: 541 LLVEKSVSRFAQEYLLAKDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENF 600
Query: 601 RLERVTEEYGTVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFA 660
R++R EE+G+ Q KK+ KTLM+FE CPK LG T+LL+G +ELKKVKHVVQY VFA
Sbjct: 601 RVDRYPEEHGSTGQVGKKVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFA 660
Query: 661 AYHLSLETSFLVDEGASLPKMPKRS---LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS 720
AYHL+LETSFL DEGAS P++P S +++ D S+S ++ V
Sbjct: 661 AYHLALETSFLADEGAS-PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTML 720
Query: 721 LSAELEESKIYPMYHHLDNNNFL----------LSTGFEEEPSIVDTFPGIFNDHSPSDV 780
AE + + P+ L L +G++ I +F +F+ H+ S
Sbjct: 721 SCAEPQRANSVPVSELLSTTTNLSIQKDIPPIPYGSGWQAR-EINPSF--VFSRHNISLN 780
Query: 781 GLNPSLSHYEELKDGETVPFDVRS-------------------------LSESELAETLE 840
+ + G +VP D + S++ ++
Sbjct: 781 LPDRVIESRNSDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIM 840
Query: 841 RRREKSGG---ITEFQKSESFDENDLSSECFSAADT-HQSILVSFSSHCVLKGTVCERSR 900
+ +G I + Q +E E E F + + HQSILVS SS V KGTVCERS
Sbjct: 841 VENQDNGSELTIAQQQNNEKPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSH 900
Query: 901 LLRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSI 960
L RIK+YG FDKPLG+FL D LF Q+ CRSC+ +EAHV CYTH+QG+LTI+V++L
Sbjct: 901 LFRIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDY 960
Query: 961 KLPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRV 1020
LPGE++G+IWMWHRCL+C +++G PPAT RV+MSDAAWGLSFGKFLELSFSNHA A+RV
Sbjct: 961 LLPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRV 1020
Query: 1021 ATCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEII 1080
A CGHSL RDCLRFYGFG+MVA FRY+TID+ SV LPPS+L FN + Q+WI +E E+I
Sbjct: 1021 ACCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFN-YENQDWIQRETDEVI 1080
Query: 1081 GKMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFM 1140
+ E L++E+ + + ++ +K + + +L+E++QKE++++ ++ +
Sbjct: 1081 ERAELLFSEVLNAISQIAEKG------------FRRRIGELEEVLQKEKAEFEENMQKIL 1140
Query: 1141 MEPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLK 1200
G VDILEL R+ R LL ++ WDHRL + +L K ++ K L
Sbjct: 1141 HREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKREENEKPPLA 1200
Query: 1201 RDSAQSDDKIEFGCNENIHESCLVHKSAGN------DLQSMQKEEPTAVECSKSTLHQVC 1260
+ +Q+ ++ G N + S + G+ L ++QKE T S L+Q
Sbjct: 1201 K--SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADT-----NSDLYQ-- 1260
Query: 1261 REEEHTDEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGA 1320
+++ ++ + +P L ++D + S+ + +TL A +
Sbjct: 1261 ------EKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQ 1320
Query: 1321 TK-QMSQNDNPQLRRVLMS------PVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFH 1380
T ++ N+ L MS P+ + Q+ K +P + L + +++
Sbjct: 1321 TSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPS-KNYE 1380
Query: 1381 ASADYNSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQ 1440
S D S L P + R+ ++ +QKL+ +P +IS+ E G RLLLP
Sbjct: 1381 NSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLP- 1440
Query: 1441 TGNIDAVIAVYDNEPTSIIAYALNSREYDDWVA---NKFTDNEGGRNVPRIIEDSRPSTL 1500
G D V+ VYD+EPTS+IAYAL S EY + N+PR ++D +
Sbjct: 1441 VGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDD---TIF 1500
Query: 1501 SAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDP---KKSPHLRISFTDESSTSGGKVNF 1560
+S G+++ + + S T DP K+ H R+S+ ++ + GKV +
Sbjct: 1501 DPSRSNGSVDESILSISSSRS-------TSLLDPLSYTKALHARVSYGEDGTL--GKVKY 1560
Query: 1561 SVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGG--------------------- 1620
+VTCY+AK+F+ LR C P ++++RSLSRC+KW AQGG
Sbjct: 1561 TVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTK 1620
Query: 1621 -------------------------------------------KSGKEVKMDLMVMENLF 1676
KSGKE KMD+++MENL
Sbjct: 1621 TELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLL 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy Swiss-Prot
Match:
Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)
HSP 1 Score: 1040.4 bits (2689), Expect = 2.2e-302
Identity = 704/1824 (38.60%), Postives = 1002/1824 (54.93%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
M D ++ V+SWI +SS +S +F P++S +C ECD F+ + + C+
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPRKSESS-NMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
C + C C S SD K +E + + C +C + E G + S
Sbjct: 61 LCGRVFCAKC--AANSIPSPSDETKDSHEEPERIRVCNYCYK---QWEQGIVPPDNGASI 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAA---SNGLLSLFN 180
+S +S SPS R + + + D S +P A+ S+ + S +
Sbjct: 121 ISLHFS---SSPSARSVASTTSNSSNCTIDSTAG--PSPRPKMNPRASRRVSSNMDSEKS 180
Query: 181 DHPSPISVHHSPNWSDEDDGEDSGRHFF---SPSSDYFRDSSDIESS----SISTRNEFF 240
+ + S S ++ D D+ FF S SD D D S S + N+++
Sbjct: 181 EQQNAKSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQSYAQGNDYY 240
Query: 241 SS-HFEESSPSASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSS 300
+ + +E + K + + P Q L+ L E ++++ ++ ++
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTET---IDKTRQQENGWND 300
Query: 301 VLPSTQNQDENAE-TLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFS 360
V + +E+ E ++DFE++ +W PP P++E DE E D ++ D GD G +
Sbjct: 301 VKEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRP 360
Query: 361 ASRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK--SGGDSVEDWVDVI 420
++ F +K +++ ++ +V+GHFRALVAQLL+ +++ + GD E W+D+I
Sbjct: 361 SNSFNEKDFHSK----DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDE-EGWLDII 420
Query: 421 TTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQ 480
T+++W+AA ++PDTS+ G MDPG Y+KVKC+ G ES ++KGVVC KN+ H+RMTS+
Sbjct: 421 TSLSWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSK 480
Query: 481 FKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSR 540
+ RLL+LGG+LEY +S+ L+SF+TLL QE DHLK ++KI+S P++LLVEKSVS
Sbjct: 481 IEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRF 540
Query: 541 AKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYG 600
A+EYLLAK+ISLVLN+KR LLERI+RCTGA + PSID ++ +LG+C+LF +E+ E +
Sbjct: 541 AQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHV 600
Query: 601 TVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSF 660
+ Q KKM+KTLMFF+GCPK LGCT+LLKG +ELKKVKHV+QY VFAAYHL+LETSF
Sbjct: 601 SPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSF 660
Query: 661 LVDEGASLPKMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS------------- 720
L DEGAS+ ++P ++ + PD N + G+ VSS
Sbjct: 661 LADEGASIHELP-----LQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEP 720
Query: 721 ------LSAELEESKIYPMYHHLDNNNFL---------LSTGFEEEPSIVDTFPGIFNDH 780
L+ SK + LD N+ + L T + + P +
Sbjct: 721 HKANGDLTGNFTSSKTH-FQGKLDGNDRIDPSERLLHNLDTVYCKPPETI---------- 780
Query: 781 SPSDVGLNPS-----LSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGITEFQKS 840
+ D GL P+ LS + E + + V S + ++ + G F +
Sbjct: 781 TSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQ 840
Query: 841 ESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLC 900
E + + + +A HQSILVS S+ CV KG+VCER+ LLRIK+YG FDKPLG+FL
Sbjct: 841 EQMESS--KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLR 900
Query: 901 DDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKC 960
D+LF Q C SC AEAH+ CYTH+QG+LTI+V++LP + LPG+ +G+IWMWHRCLKC
Sbjct: 901 DNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKC 960
Query: 961 AQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGS 1020
+I+G PPAT R++MSDAAWGLSFGKFLELSFSNHA A+RVA CGHSL RDCLRFYGFG
Sbjct: 961 PRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGR 1020
Query: 1021 MVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQ 1080
MVA FRY++I+I +VTLPP+ L FN + QEW+ KE+ E+I K E L+ E+ + + ++
Sbjct: 1021 MVACFRYASINIYAVTLPPAKLYFN-YENQEWLQKESKEVIKKAEVLFNEVQEALSQISA 1080
Query: 1081 KSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRL 1140
K+ + + S ++++ + +L L+++ + +Y L+ M+ G ++DIL +N+L
Sbjct: 1081 KTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQ-MLNVVKDGQPTIDILLINKL 1140
Query: 1141 RRSLLIHTHYWDHRLYSLDSLLKK--ISNPKADVSSPLKRVLKRDSAQSDDKIE------ 1200
RR ++ ++ WD L ++++ + PK + R + + SD+K++
Sbjct: 1141 RRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKL-SDEKVKSIPTHV 1200
Query: 1201 FGCNENI-----HESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDE---- 1260
CN+++ +E+CL + D + E S CR E E
Sbjct: 1201 AICNDSLLQDADYETCLNQGKSFADTSG----KFAIPEDVGSDRPPDCRMEFDPSEGGKD 1260
Query: 1261 ---EMTAVIASIEGIPSYEST-LSERIDCAWSG----SEQSVTKP--QAVSTLQAQEPPD 1320
E + V+ +++T LS+ +D AW G SE + +P +A ST Q P
Sbjct: 1261 NFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP-- 1320
Query: 1321 GATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADY 1380
+R+ +S + + H + QL + SF+ S +
Sbjct: 1321 --------------------DLRLLGSESELNFKGGPTNDEHTTQVQLPS-PSFYYSLNK 1380
Query: 1381 NSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQTGNID 1440
N L +R H ++ P ++S+ E G RLLLP G D
Sbjct: 1381 NYSLN------SRKH------------IMAEDRPVYVSSYRELEWRSGARLLLP-LGCND 1440
Query: 1441 AVIAVYDNEPTSIIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGA 1500
V+ VYD+EPTSIIAYAL S EY ++ G + R DS S S + S
Sbjct: 1441 LVLPVYDDEPTSIIAYALTSSEYKAQMS--------GSDKSRDRLDSGGS-FSLFDSVNL 1500
Query: 1501 LNLDYIH------YGNYGSEDPTTLRTLFSD-PKKSPHLRISFTDESSTSGGKVNFSVTC 1560
L+L+ + + S D + L S K H RISFTDE GKV +SVTC
Sbjct: 1501 LSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPP--GKVKYSVTC 1560
Query: 1561 YFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS----------------------- 1620
Y+AK+F+ LR CCP DF+RSL RCRKW AQGGKS
Sbjct: 1561 YYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELE 1620
Query: 1621 -----------------------------------------GKEVKMDLMVMENLFFMRN 1676
GKE KMD++VMENL F RN
Sbjct: 1621 SFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRN 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy Swiss-Prot
Match:
Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)
HSP 1 Score: 626.3 bits (1614), Expect = 9.8e-178
Identity = 469/1486 (31.56%), Postives = 740/1486 (49.80%), Query Frame = 0
Query: 293 STQNQDENAETLLDFENND---RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSA 352
S +N EN + L D E++ +W PP P++ DE++ FA DD+D G S
Sbjct: 72 SKENSVENVQFLSDREDDSDDVPVWEPPEPENPEDEVD-GVFADDDDDCCDGSKWNKASL 131
Query: 353 SRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVED---WVDVI 412
LS K+ ++EEN+ + F+ +V+QL IKS G S+E+ W +++
Sbjct: 132 LGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQL-----IKSAGFSIEESGYWFEIV 191
Query: 413 TTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQ 472
+ W+AA+ ++P G S+DP +YIKVKC+A+G +S + KG+V K+ K M ++
Sbjct: 192 ARLCWEAASMLKPAID-GKSVDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATK 251
Query: 473 FKNARLLLLGGSLEYHEVSDHLASFNTL--LHQENDHL----KTIISKIESLRPNVLLVE 532
+++ R++L+ G L + ++ F++L ++Q+N++L K ++ IE+ +P+V+LVE
Sbjct: 252 YEHPRIMLVEGVLGH-----PISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVE 311
Query: 533 KSVSSRAKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLER 592
KSVS ++ +L K ++LV ++K L+RI+RC G+ + S+D +S +L HC+ FR+E+
Sbjct: 312 KSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEK 371
Query: 593 VTEEYGTVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHL 652
+ EE+ + +KK +KTLMF EGCP RLGCT+LLKG E LKKVK VVQY+ AYHL
Sbjct: 372 IVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHL 431
Query: 653 SLETSFLVDEGASLPKMPKRS-----LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLS 712
LE SFL D + + + +E+ S S +P + S + VS G+ ++
Sbjct: 432 MLEASFLADRHTMFSTIFAKEATSCVVEIENFSPSPSPRESPSEAVDIPVSNGFDEQTIQ 491
Query: 713 AELE---------ESKIYPMYHHLDNNNFLLSTGFEEEPSIVDTFPGIFNDHSPSDVGLN 772
E ES ++ H + N ++ TGF + + + G + V ++
Sbjct: 492 INGEADGEKVGTWESDGDHVFSH-EPYNPVIFTGFSSLSARLSKYLGFVQNPESVPVSVD 551
Query: 773 PSLSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGI--TEFQKSESFDENDLSSE 832
+S L S+ ESE +T E+ +K + E + S D+ D S+
Sbjct: 552 TDVSTTSNLD----------SIRESE-EDTAEKNEDKQPLLLDPELPVNSSSDDGDNKSQ 611
Query: 833 CFSAADT---HQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLCDDLFAQTS 892
+ ++ QSILV S L+G +C++ IKFY FD PL KFL D+F Q +
Sbjct: 612 TENDIESTLESQSILVLVSKRNALRGIMCDQRHFSHIKFYKHFDVPLEKFL-RDMFNQRN 671
Query: 893 SCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIK-LPGEEDGRIWMWHRCLKCAQIDGVP 952
C++C E EAH+ Y HQ LTI ++R+P K L GE G+IWMW RC KC +
Sbjct: 672 LCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASR 731
Query: 953 PATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGSMVAFFRY 1012
+T RV++S AA LSFGKFLELSFS NR ++CGHS D L F+G GSMVA Y
Sbjct: 732 KSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSY 791
Query: 1013 STIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQKSKLSMQ 1072
S + +V+LPP LE + ++ W+ KE + K SL+ + + + R+ + S
Sbjct: 792 SQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLFEDAAGFLKRLRSQFTNSDL 851
Query: 1073 ESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRLRRSLLIH 1132
+ ++ S++ +EL++ ER + +K + + +S +L LNR+R LL+
Sbjct: 852 RYQRARKLLSNI---EELLKHERCIFEENIKNSFDKAKTIDDVSHRLLRLNRMRWELLLQ 911
Query: 1133 THYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSDDKIEFGCNEN-IHESCLVH 1192
W++RL SL +L P +D + ++ LK S + E N+N + +S
Sbjct: 912 ALIWNYRLQSL--VLSDRLLPSSDETKIYEQGLKTVSEAGMTRYE---NDNKVSDS---G 971
Query: 1193 KSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAVIASIEGIPSYESTLSERI 1252
+ G D ++ ++ S Q+ D E + +S P S ++
Sbjct: 972 SNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVPEDNESQTLCSS---SPDTTSPINNHF 1031
Query: 1253 DCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRM 1312
D + + S +A ++ + ++S ++ P + +D +
Sbjct: 1032 DTHLAVNVHSTNGQEADKSIPV--TGESLDDEVSTSNGP----------HILGWDEWFWL 1091
Query: 1313 Q-ERIRKGLHPSTFQLSTIRSFHASADY-NSMLRDPVSSLTRAHSQVLPFETQKLNLLLS 1372
E +R S + + + +Y N+ ++ + ++ +Q++ E+ +L + L
Sbjct: 1092 PFEELR-----SKRIVDIEKEYLLKFEYVNNFTQENLQTV----NQIITEESSRLRISLR 1151
Query: 1373 STPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYALNSREYDDWVANKFTDN 1432
D +++ Y++E +S+IA AL +
Sbjct: 1152 DD-----------------------DFIVSDYEDELSSLIACAL----------AHLNNE 1211
Query: 1433 EGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDPKKSPHLRI 1492
E + + R I S L Q + D + SE L TL P +
Sbjct: 1212 ESKKPLSRCIHGSLQGFLDNNQDSKQTDRD---VSRFSSESTNRLETL-------PPPEV 1271
Query: 1493 SFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS---- 1552
T S S GK +S+ +A F LRK+CC +D++ SLSRC+ W A+GGKS
Sbjct: 1272 LVTFGSVKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVF 1331
Query: 1553 ------------------------------------------------------------ 1612
Sbjct: 1332 AKTLDDRFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKG 1391
Query: 1613 -GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYN-PDTTGADKVLLDQNLLEALRTKP 1672
GKE++ DLMVMENL F R +++ YDLKG+ +R+ G D VLLDQN + + P
Sbjct: 1392 GGKEIRHDLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSP 1451
Query: 1673 IFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLGIIDFMRQYTWDKQL 1677
+++ +K+NL+RA++NDT FL SI+VMDYSLLVGVD+E ELV GIID++RQYTWDKQL
Sbjct: 1452 LYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQL 1451
BLAST of Cp4.1LG01g06240 vs. ExPASy Swiss-Prot
Match:
P34756 (1-phosphatidylinositol 3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FAB1 PE=1 SV=3)
HSP 1 Score: 352.1 bits (902), Expect = 3.6e-95
Identity = 373/1580 (23.61%), Postives = 643/1580 (40.70%), Query Frame = 0
Query: 363 LHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPDTS 422
L +E K L + H AL+ QLL ++I ++++W+ ++ + +
Sbjct: 780 LKKEKKRELNEVSLLHMHALLKQLLNDQEI----SNLQEWITLLDGALRKVLRTILNARD 839
Query: 423 RGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLEYH 482
Y+K+K ++ G P+ S I GVV +K + K M KN R+LL+ LEY
Sbjct: 840 LNTLDFRQTYVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQ 899
Query: 483 EVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVLNV 542
+ ++H S ++ QE ++L ++S+++SL P+++ V +VS A E L I + N+
Sbjct: 900 KNNNHFLSIESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNM 959
Query: 543 KRPLLERIARCTGASLTPSIDHMSIS-RLGHCELFRLERVTEEYGTVNQNKKMSKTLMFF 602
K ++ERIA+ T A + S+D ++ + ++G CE F ++ YG + SKT F
Sbjct: 960 KPQVIERIAKLTEADIAISVDKLATNIKMGECETFEVKSYI--YGNI------SKTYTFL 1019
Query: 603 EGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRSL 662
GC LG T+LL+G E L+K+K V ++ V+A + L LE+SF D L
Sbjct: 1020 RGCNPELGGTILLRGDSLENLRKIKQVSEFMVYAIFSLKLESSFFNDNFIQL-------- 1079
Query: 663 SVEDNSSSGNPDVTMSSNCLVDVSTGY------KVSSLSAELEESKIYPMYHHLDNNNFL 722
S + + + + + V GY K ++ + + +P+ FL
Sbjct: 1080 -------STDVYLKRAESKKLQVFEGYFADFLIKFNNRILTVSPTVDFPI-------PFL 1139
Query: 723 LSTGFEEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGET---VPFDVRSLSESEL 782
L E +++ I S SD+ L+ + L+ T + ++ L E E+
Sbjct: 1140 LEKARGLEKKLIER---INQYESESDLDRQTQLNMLQGLESTITKKHLGNLIKFLHEMEI 1199
Query: 783 AETLERRREKSGGITEFQK-SESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCER 842
E LE EFQK S ++ + SS+ +HQSI V +S T C
Sbjct: 1200 -ENLE---------LEFQKRSRQWEVSYSSSQNLLGTGSHQSITVLYSMVSTKTATPCVG 1259
Query: 843 SRLLRIKFYGRFDKPLGKFLCDDLFAQTSSC-RSCKESAEAHVQCYTHQQGNLTINVRRL 902
+++ I ++ D +G+F+ + + C + C H + Y H G + + + +
Sbjct: 1260 PQIVTIDYFWDSDISIGQFIENVVGTARYPCQQGCNGLYLDHYRSYVHGSGKVDVLIEKF 1319
Query: 903 PSIKLPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSF-SNHAT 962
+ +LP +D I W C KC +TP + +S+ W SFGK+LE+ F S +
Sbjct: 1320 QT-RLPKLKD-IILTWSYCKKCG------TSTPILQISEKTWNHSFGKYLEVMFWSYKDS 1379
Query: 963 ANRVATCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEA 1022
+ C H +D ++++G+ +V YS +++ + PP +++ H+ + +
Sbjct: 1380 VTGIGKCPHDFTKDHVKYFGYNDLVVRLEYSDLEVHELITPPRKIKWKPHIDIKLKVELY 1439
Query: 1023 SEIIGKMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLL 1082
+I+ K+ + Y + ++R+ KL Q+ +++LK +E+ + L
Sbjct: 1440 YKILEKINNFYGSVLSRLERI----KLDSMTKDKVLSGQAKIIELKSNATEEQKLMLQDL 1499
Query: 1083 KPFMMEPSLLGMISVDIL---------------------------ELNRLRRSLLIHTHY 1142
F + + ++++ +++R+ L Y
Sbjct: 1500 DTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIFAKSYLPSETDISRITAKQLKKLFY 1559
Query: 1143 WDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSDDKIEFGCNENIHESCLVHKSAG 1202
R S D K + + KA P K L + + +K + ++N E+
Sbjct: 1560 DSSRKDSEDK--KSLHDEKAKTRKPEKNELPLEGLKDVEKPKID-SKNTTENRDRTNEPQ 1619
Query: 1203 NDLQSMQKEEPTAVECSKSTLH--------QVCREEEHTDEEMTAVIASIEGI------- 1262
N + ++ T + + T H V EE + S GI
Sbjct: 1620 NAVTITTFKDDTPIIPTSGTSHLTVTPSASSVSSSLTPQTEERPPISRSGTGISMTHDKS 1679
Query: 1263 --PSYESTLSERIDCAWSGSEQSVTKPQAVSTLQA---QEPPDGATKQMS---QNDNPQL 1322
P+ S+ C + +K VS L Q D +K+ + + QL
Sbjct: 1680 TRPNIRKMSSDSSLCGLASLANEYSKNNKVSKLATFFDQMHFDALSKEFELERERERLQL 1739
Query: 1323 RRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRA 1382
+ +R+ + + + + ++ + S+ + +SA+ + L PV +RA
Sbjct: 1740 NKDKYQAIRLQTSTPIVEIYKNVKDAVDEPLHSRSSGNNL-SSANVKT-LEAPVGEHSRA 1799
Query: 1383 HSQVLPFETQKL-------------NLL-----------LSSTPSFISASHVPEGV---- 1442
++ P Q L N+L L+S P S P+ +
Sbjct: 1800 NNCNPPNLDQNLETELENSISQWGENILNPSGKTTASTHLNSKPVVKETSENPKSIVRES 1859
Query: 1443 ----------------------------RLLLPQTGNI---------------------- 1502
LL+ N
Sbjct: 1860 DNSKSEPLPPVITTTTVNKVESTPQPEKSLLMKTLSNFWADRSAYLWKPLVYPTCPSEHI 1919
Query: 1503 --DAVIAVYDNEPTSIIAYALNSREY---------------------------------- 1562
D+ + + ++EP+S+IA+ L++ +Y
Sbjct: 1920 FTDSDVIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQ 1979
Query: 1563 ---------------------DDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALN 1622
D +++ T EG N P IE +R T Q+ N
Sbjct: 1980 TGDPSVNISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPP-IEGARDRTPQESQTHSQAN 2039
Query: 1623 LDYIHYGNYGSEDPTTLRTL--FSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFD 1676
LD TL+ L K + HLR F + G S +F + FD
Sbjct: 2040 LD-------------TLQELEKIMTKKTATHLRYQFEE------GLTVMSCKIFFTEHFD 2099
BLAST of Cp4.1LG01g06240 vs. NCBI nr
Match:
XP_023514916.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita pepo subsp. pepo])
HSP 1 Score: 3304 bits (8568), Expect = 0.0
Identity = 1677/1741 (96.32%), Postives = 1677/1741 (96.32%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS
Sbjct: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP
Sbjct: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN
Sbjct: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
Query: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK
Sbjct: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
Query: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD
Sbjct: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
Query: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE
Sbjct: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
Query: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL
Sbjct: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
Query: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF
Sbjct: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
Query: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS
Sbjct: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
Query: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF
Sbjct: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
Query: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR
Sbjct: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
Query: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY
Sbjct: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
Query: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED
Sbjct: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
Query: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL
Sbjct: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
Query: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY
Sbjct: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
Query: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG
Sbjct: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
Query: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD
Sbjct: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
Query: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV
Sbjct: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
Query: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR
Sbjct: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
Query: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH
Sbjct: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
Query: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL
Sbjct: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
Query: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT
Sbjct: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
Query: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS
Sbjct: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
Query: 1501 RCRKWSAQGGKS------------------------------------------------ 1560
RCRKWSAQGGKS
Sbjct: 1501 RCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSQALNSGSPTCL 1560
Query: 1561 ----------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV
Sbjct: 1561 AKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
Query: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1677
LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG
Sbjct: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1680
BLAST of Cp4.1LG01g06240 vs. NCBI nr
Match:
KAG7030579.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 3268 bits (8474), Expect = 0.0
Identity = 1656/1740 (95.17%), Postives = 1668/1740 (95.86%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECD SFSKTSLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDTSFSKTSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS
Sbjct: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDD+KHPFSSVLPSTQNQDEN
Sbjct: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDNKHPFSSVLPSTQNQDEN 300
Query: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
AETLLDFENNDRIWFPPPPDDE+DELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK
Sbjct: 301 AETLLDFENNDRIWFPPPPDDENDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
Query: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD
Sbjct: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
Query: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE
Sbjct: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
Query: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
YHEVSDHLASFNTLLHQENDHLKTIIS+IESLRPNVLLVEKSVSSRA+EYLLAKEISLVL
Sbjct: 481 YHEVSDHLASFNTLLHQENDHLKTIISRIESLRPNVLLVEKSVSSRAQEYLLAKEISLVL 540
Query: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF
Sbjct: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
Query: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS
Sbjct: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
Query: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNFLLSTGF
Sbjct: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNFLLSTGF 720
Query: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
EEE SIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR
Sbjct: 721 EEESSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
Query: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY
Sbjct: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
Query: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED
Sbjct: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
Query: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL
Sbjct: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
Query: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHV+QEWISKEASEIIGKMESLY
Sbjct: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVRQEWISKEASEIIGKMESLY 1020
Query: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
AEISDIVDRMEQKSKLSMQE SDSSEMQSH+MQLKELIQKERSDYIGLLKPFMMEPSLLG
Sbjct: 1021 AEISDIVDRMEQKSKLSMQEPSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
Query: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKK+SNPK D+SSPLKRVLKRDSA SD
Sbjct: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKLSNPKTDISSPLKRVLKRDSALSD 1140
Query: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
DKIEFGCNENIHES VHKSAGNDLQSMQKEEPTA+ECSKSTLHQVCREEEHTDEEMTAV
Sbjct: 1141 DKIEFGCNENIHESSSVHKSAGNDLQSMQKEEPTALECSKSTLHQVCREEEHTDEEMTAV 1200
Query: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR
Sbjct: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
Query: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH
Sbjct: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
Query: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL
Sbjct: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
Query: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
NSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT
Sbjct: 1381 NSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
Query: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS
Sbjct: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
Query: 1501 RCRKWSAQGGKS------------------------------------------------ 1560
RCRKWSAQGGKS
Sbjct: 1501 RCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSQALNSGSPTCL 1560
Query: 1561 ----------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV
Sbjct: 1561 AKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
Query: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1676
LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG
Sbjct: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1680
BLAST of Cp4.1LG01g06240 vs. NCBI nr
Match:
KAG6599898.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 3248 bits (8421), Expect = 0.0
Identity = 1649/1740 (94.77%), Postives = 1660/1740 (95.40%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECD SFSKTSLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDTSFSKTSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCR CIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGR SEKVYSS
Sbjct: 61 SCSQSSCRKCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRQSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYKSP KTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKSPMKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAE ILNRSDD+KHPFSSVLPSTQN DEN
Sbjct: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAEAILNRSDDNKHPFSSVLPSTQNLDEN 300
Query: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
A TLLDFENN RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK
Sbjct: 301 AGTLLDFENNGRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
Query: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD
Sbjct: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
Query: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
TSRGGSMDPGDYIKVKCVASGH RESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE
Sbjct: 421 TSRGGSMDPGDYIKVKCVASGHLRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
Query: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
YHEVSDHLASFNTLLHQENDHLKTIIS+IESLRPNVLLVEKSVSSRA+EYLLAKEISLVL
Sbjct: 481 YHEVSDHLASFNTLLHQENDHLKTIISRIESLRPNVLLVEKSVSSRAQEYLLAKEISLVL 540
Query: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF
Sbjct: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
Query: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS
Sbjct: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
Query: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNFLLSTGF
Sbjct: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNFLLSTGF 720
Query: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
EEE SIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR
Sbjct: 721 EEESSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
Query: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY
Sbjct: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
Query: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED
Sbjct: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
Query: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL
Sbjct: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
Query: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHV+QEWISKEASEIIGKMESLY
Sbjct: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVRQEWISKEASEIIGKMESLY 1020
Query: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
AEISDIVDRMEQKSKLSMQE SDSSEMQSH+MQLKELIQKERSDYIGLLKPFMMEPSLLG
Sbjct: 1021 AEISDIVDRMEQKSKLSMQEPSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
Query: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKK+SNPK D+SSPLKRVLKRDSA SD
Sbjct: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKLSNPKTDISSPLKRVLKRDSALSD 1140
Query: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
DKIEFGCNENIHES VHKSAGNDLQSMQKEEPTA+ECSKSTLHQVCREEEHTDEEMTAV
Sbjct: 1141 DKIEFGCNENIHESSSVHKSAGNDLQSMQKEEPTALECSKSTLHQVCREEEHTDEEMTAV 1200
Query: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR
Sbjct: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
Query: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH
Sbjct: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
Query: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL
Sbjct: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
Query: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
NSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT
Sbjct: 1381 NSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
Query: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS
Sbjct: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
Query: 1501 RCRKWSAQGGKS------------------------------------------------ 1560
RCRKWSAQGGKS
Sbjct: 1501 RCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSQALNSGSPTCL 1560
Query: 1561 ----------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV
Sbjct: 1561 AKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
Query: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1676
LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG
Sbjct: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1680
BLAST of Cp4.1LG01g06240 vs. NCBI nr
Match:
XP_022941900.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita moschata])
HSP 1 Score: 3237 bits (8392), Expect = 0.0
Identity = 1646/1740 (94.60%), Postives = 1659/1740 (95.34%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECD SFSKTSLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDTSFSKTSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCR CIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGR SEKVYSS
Sbjct: 61 SCSQSSCRKCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRQSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYKSP KTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKSPMKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAE ILNRSDD+KHPFSSVLPSTQN DEN
Sbjct: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAEAILNRSDDNKHPFSSVLPSTQNLDEN 300
Query: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
A TLLDFENN RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK
Sbjct: 301 AGTLLDFENNGRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
Query: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD
Sbjct: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
Query: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
TSRGGSMDPGDYIKVKCVASGH RESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE
Sbjct: 421 TSRGGSMDPGDYIKVKCVASGHLRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
Query: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
YHEVSDHLASFNTLLHQENDHLKTIIS+IESLRPNVLLVEKSVSSRA+EYLLAKEISLVL
Sbjct: 481 YHEVSDHLASFNTLLHQENDHLKTIISRIESLRPNVLLVEKSVSSRAQEYLLAKEISLVL 540
Query: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYG+V+QNKKMSKTLMF
Sbjct: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGSVDQNKKMSKTLMF 600
Query: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS
Sbjct: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
Query: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNFLLSTGF
Sbjct: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNFLLSTGF 720
Query: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
EEE SIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR
Sbjct: 721 EEESSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
Query: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY
Sbjct: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
Query: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED
Sbjct: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
Query: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL
Sbjct: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
Query: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPS+LEFNSHV+QEWISKEASEIIGKMESLY
Sbjct: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSMLEFNSHVRQEWISKEASEIIGKMESLY 1020
Query: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
AEISDIVDRMEQKSKLSMQESSDSSEMQSH+MQLKELIQKERSDYIGLLKPFMMEPSLLG
Sbjct: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
Query: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
MISVDILELN LRRSLLIHTHYWDHRLYSLDSLLKK+SNPK DVSSPLKRVLKRDSAQSD
Sbjct: 1081 MISVDILELNCLRRSLLIHTHYWDHRLYSLDSLLKKLSNPKTDVSSPLKRVLKRDSAQSD 1140
Query: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
DKIEFGCNENIHES VHKSAGNDLQSM KEEPTA+ECSKSTLHQVCREE HTDEEMTAV
Sbjct: 1141 DKIEFGCNENIHESSSVHKSAGNDLQSMPKEEPTALECSKSTLHQVCREE-HTDEEMTAV 1200
Query: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR
Sbjct: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
Query: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH
Sbjct: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
Query: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL
Sbjct: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
Query: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
NSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT
Sbjct: 1381 NSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
Query: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS
Sbjct: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
Query: 1501 RCRKWSAQGGKS------------------------------------------------ 1560
RCRKWSAQGGKS
Sbjct: 1501 RCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSQALNSGSPTCL 1560
Query: 1561 ----------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSR+RYNPDTTGADKV
Sbjct: 1561 AKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRARYNPDTTGADKV 1620
Query: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1676
LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG
Sbjct: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1680
BLAST of Cp4.1LG01g06240 vs. NCBI nr
Match:
XP_022990385.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita maxima])
HSP 1 Score: 3211 bits (8324), Expect = 0.0
Identity = 1637/1747 (93.70%), Postives = 1650/1747 (94.45%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLS+EFVA NKSYKMCCECD SFSK SLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSQEFVALNKSYKMCCECDTSFSKNSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCRNCIRGYESYVVQSDVMK GNEAVKTTKSCKFCSDGNLR EGGGRHSEKVYSS
Sbjct: 61 SCSQSSCRNCIRGYESYVVQSDVMKAGNEAVKTTKSCKFCSDGNLRREGGGRHSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYK P KTESIPDDRLCRYLESQDCGYSPYAASNGLL LFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKPPMKTESIPDDRLCRYLESQDCGYSPYAASNGLLCLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPI VHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPIYVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSD------DHKHPFSSVLPST 300
ASPSRSDYT NK DHVVQSVDPGQRLNLLPQEAETILNRS+ D+KHPFSSVLPST
Sbjct: 241 ASPSRSDYTLNKVDHVVQSVDPGQRLNLLPQEAETILNRSERLTEGHDNKHPFSSVLPST 300
Query: 301 QNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
QNQDENAETLLDFENN RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS
Sbjct: 301 QNQDENAETLLDFENNGRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
Query: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAA 420
SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK GGDSVEDWVDVITTIAWQAA
Sbjct: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKPGGDSVEDWVDVITTIAWQAA 420
Query: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLL 480
NFVRPDTSRGGSMDPGDYIKVKCVASGHPREST IKGVVCTKNIKHKRMTSQFKNARLLL
Sbjct: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTFIKGVVCTKNIKHKRMTSQFKNARLLL 480
Query: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAK 540
LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRA+EYLLAK
Sbjct: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAQEYLLAK 540
Query: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM
Sbjct: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
Query: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP
Sbjct: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
Query: 661 KMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNF 720
KMPKRSLSVEDNSSSGNPDVTMSS+CLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNF
Sbjct: 661 KMPKRSLSVEDNSSSGNPDVTMSSDCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNF 720
Query: 721 LLSTGFEEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
LLSTGFEEE SIVDTFPGIFNDHS SDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE
Sbjct: 721 LLSTGFEEESSIVDTFPGIFNDHSLSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
Query: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL
Sbjct: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
Query: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIK 900
LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKES EAHVQCYTHQQGNLTINVRRLPSIK
Sbjct: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESTEAHVQCYTHQQGNLTINVRRLPSIK 900
Query: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA
Sbjct: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
Query: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIG 1020
TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHV+QEWISKEASEIIG
Sbjct: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVRQEWISKEASEIIG 1020
Query: 1021 KMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMM 1080
KMESLYAEISDIVD MEQKSKLSMQESSDSSEMQSH+MQLKELIQKERSDYIGLLKPFMM
Sbjct: 1021 KMESLYAEISDIVDGMEQKSKLSMQESSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMM 1080
Query: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR
Sbjct: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
Query: 1141 DSAQSDDKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTD 1200
DSAQSDDKIEFGCNENIHES LVHKSAGNDLQSMQKEEPTA+ECSKSTLHQVCREEEHTD
Sbjct: 1141 DSAQSDDKIEFGCNENIHESSLVHKSAGNDLQSMQKEEPTALECSKSTLHQVCREEEHTD 1200
Query: 1201 EEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQN 1260
EE+TAVIASIEGIP YESTLSERID AWSGSEQ++TKPQAVSTLQAQEPPDGATKQMSQN
Sbjct: 1201 EEITAVIASIEGIPPYESTLSERIDYAWSGSEQAMTKPQAVSTLQAQEPPDGATKQMSQN 1260
Query: 1261 DNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVS 1320
DNP LRRVLMSPVRVHSFDSAIRMQERIRKGL PSTFQLSTIRSFHASADYNSM RDPVS
Sbjct: 1261 DNPHLRRVLMSPVRVHSFDSAIRMQERIRKGLDPSTFQLSTIRSFHASADYNSMPRDPVS 1320
Query: 1321 SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS 1380
SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS
Sbjct: 1321 SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS 1380
Query: 1381 IIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG 1440
IIAYALNSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG
Sbjct: 1381 IIAYALNSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG 1440
Query: 1441 SEDPTTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD 1500
SED TTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD
Sbjct: 1441 SEDSTTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD 1500
Query: 1501 FVRSLSRCRKWSAQGGKS------------------------------------------ 1560
FVRSLSRCRKWSAQGGKS
Sbjct: 1501 FVRSLSRCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSLALNS 1560
Query: 1561 ----------------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT
Sbjct: 1561 GSPTCLAKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT 1620
Query: 1621 TGADKVLLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER 1677
TGADKVLLDQNLLEALRTK IFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER
Sbjct: 1621 TGADKVLLDQNLLEALRTKTIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy TrEMBL
Match:
A0A6J1FV39 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111447122 PE=4 SV=1)
HSP 1 Score: 3237 bits (8392), Expect = 0.0
Identity = 1646/1740 (94.60%), Postives = 1659/1740 (95.34%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECD SFSKTSLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDTSFSKTSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCR CIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGR SEKVYSS
Sbjct: 61 SCSQSSCRKCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRQSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYKSP KTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKSPMKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPSTQNQDEN 300
ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAE ILNRSDD+KHPFSSVLPSTQN DEN
Sbjct: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAEAILNRSDDNKHPFSSVLPSTQNLDEN 300
Query: 301 AETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
A TLLDFENN RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK
Sbjct: 301 AGTLLDFENNGRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAK 360
Query: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD
Sbjct: 361 QNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPD 420
Query: 421 TSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
TSRGGSMDPGDYIKVKCVASGH RESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE
Sbjct: 421 TSRGGSMDPGDYIKVKCVASGHLRESTLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLE 480
Query: 481 YHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVL 540
YHEVSDHLASFNTLLHQENDHLKTIIS+IESLRPNVLLVEKSVSSRA+EYLLAKEISLVL
Sbjct: 481 YHEVSDHLASFNTLLHQENDHLKTIISRIESLRPNVLLVEKSVSSRAQEYLLAKEISLVL 540
Query: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMF 600
NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYG+V+QNKKMSKTLMF
Sbjct: 541 NVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGSVDQNKKMSKTLMF 600
Query: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS
Sbjct: 601 FEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRS 660
Query: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGF 720
LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNFLLSTGF
Sbjct: 661 LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNFLLSTGF 720
Query: 721 EEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
EEE SIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR
Sbjct: 721 EEESSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAETLERRR 780
Query: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY
Sbjct: 781 EKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFY 840
Query: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED
Sbjct: 841 GRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEED 900
Query: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL
Sbjct: 901 GRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSL 960
Query: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLY 1020
QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPS+LEFNSHV+QEWISKEASEIIGKMESLY
Sbjct: 961 QRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSMLEFNSHVRQEWISKEASEIIGKMESLY 1020
Query: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
AEISDIVDRMEQKSKLSMQESSDSSEMQSH+MQLKELIQKERSDYIGLLKPFMMEPSLLG
Sbjct: 1021 AEISDIVDRMEQKSKLSMQESSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMMEPSLLG 1080
Query: 1081 MISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSD 1140
MISVDILELN LRRSLLIHTHYWDHRLYSLDSLLKK+SNPK DVSSPLKRVLKRDSAQSD
Sbjct: 1081 MISVDILELNCLRRSLLIHTHYWDHRLYSLDSLLKKLSNPKTDVSSPLKRVLKRDSAQSD 1140
Query: 1141 DKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAV 1200
DKIEFGCNENIHES VHKSAGNDLQSM KEEPTA+ECSKSTLHQVCREE HTDEEMTAV
Sbjct: 1141 DKIEFGCNENIHESSSVHKSAGNDLQSMPKEEPTALECSKSTLHQVCREE-HTDEEMTAV 1200
Query: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR
Sbjct: 1201 IASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLR 1260
Query: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH
Sbjct: 1261 RVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAH 1320
Query: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL
Sbjct: 1321 SQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYAL 1380
Query: 1381 NSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
NSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT
Sbjct: 1381 NSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTT 1440
Query: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS
Sbjct: 1441 LRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLS 1500
Query: 1501 RCRKWSAQGGKS------------------------------------------------ 1560
RCRKWSAQGGKS
Sbjct: 1501 RCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSQALNSGSPTCL 1560
Query: 1561 ----------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKV 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSR+RYNPDTTGADKV
Sbjct: 1561 AKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRARYNPDTTGADKV 1620
Query: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1676
LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG
Sbjct: 1621 LLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLG 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy TrEMBL
Match:
A0A6J1JPY8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111487234 PE=4 SV=1)
HSP 1 Score: 3211 bits (8324), Expect = 0.0
Identity = 1637/1747 (93.70%), Postives = 1650/1747 (94.45%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVRSWISWGPDSSVCLS+EFVA NKSYKMCCECD SFSK SLVYLCQ
Sbjct: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSQEFVALNKSYKMCCECDTSFSKNSLVYLCQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SCSQSSCRNCIRGYESYVVQSDVMK GNEAVKTTKSCKFCSDGNLR EGGGRHSEKVYSS
Sbjct: 61 SCSQSSCRNCIRGYESYVVQSDVMKAGNEAVKTTKSCKFCSDGNLRREGGGRHSEKVYSS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPEAPSPSY GTYK P KTESIPDDRLCRYLESQDCGYSPYAASNGLL LFNDHP
Sbjct: 121 VSPRYSPEAPSPSYHGTYKPPMKTESIPDDRLCRYLESQDCGYSPYAASNGLLCLFNDHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
SPI VHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS
Sbjct: 181 SPIYVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSD------DHKHPFSSVLPST 300
ASPSRSDYT NK DHVVQSVDPGQRLNLLPQEAETILNRS+ D+KHPFSSVLPST
Sbjct: 241 ASPSRSDYTLNKVDHVVQSVDPGQRLNLLPQEAETILNRSERLTEGHDNKHPFSSVLPST 300
Query: 301 QNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
QNQDENAETLLDFENN RIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS
Sbjct: 301 QNQDENAETLLDFENNGRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
Query: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAA 420
SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK GGDSVEDWVDVITTIAWQAA
Sbjct: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKPGGDSVEDWVDVITTIAWQAA 420
Query: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLL 480
NFVRPDTSRGGSMDPGDYIKVKCVASGHPREST IKGVVCTKNIKHKRMTSQFKNARLLL
Sbjct: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTFIKGVVCTKNIKHKRMTSQFKNARLLL 480
Query: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAK 540
LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRA+EYLLAK
Sbjct: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAQEYLLAK 540
Query: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM
Sbjct: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
Query: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP
Sbjct: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
Query: 661 KMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNF 720
KMPKRSLSVEDNSSSGNPDVTMSS+CLVDVSTGYKVS+LSAELEESKIYPMYHHLDNNNF
Sbjct: 661 KMPKRSLSVEDNSSSGNPDVTMSSDCLVDVSTGYKVSALSAELEESKIYPMYHHLDNNNF 720
Query: 721 LLSTGFEEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
LLSTGFEEE SIVDTFPGIFNDHS SDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE
Sbjct: 721 LLSTGFEEESSIVDTFPGIFNDHSLSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
Query: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL
Sbjct: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
Query: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIK 900
LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKES EAHVQCYTHQQGNLTINVRRLPSIK
Sbjct: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESTEAHVQCYTHQQGNLTINVRRLPSIK 900
Query: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA
Sbjct: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
Query: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIG 1020
TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHV+QEWISKEASEIIG
Sbjct: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVRQEWISKEASEIIG 1020
Query: 1021 KMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMM 1080
KMESLYAEISDIVD MEQKSKLSMQESSDSSEMQSH+MQLKELIQKERSDYIGLLKPFMM
Sbjct: 1021 KMESLYAEISDIVDGMEQKSKLSMQESSDSSEMQSHIMQLKELIQKERSDYIGLLKPFMM 1080
Query: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR
Sbjct: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
Query: 1141 DSAQSDDKIEFGCNENIHESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTD 1200
DSAQSDDKIEFGCNENIHES LVHKSAGNDLQSMQKEEPTA+ECSKSTLHQVCREEEHTD
Sbjct: 1141 DSAQSDDKIEFGCNENIHESSLVHKSAGNDLQSMQKEEPTALECSKSTLHQVCREEEHTD 1200
Query: 1201 EEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQN 1260
EE+TAVIASIEGIP YESTLSERID AWSGSEQ++TKPQAVSTLQAQEPPDGATKQMSQN
Sbjct: 1201 EEITAVIASIEGIPPYESTLSERIDYAWSGSEQAMTKPQAVSTLQAQEPPDGATKQMSQN 1260
Query: 1261 DNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPVS 1320
DNP LRRVLMSPVRVHSFDSAIRMQERIRKGL PSTFQLSTIRSFHASADYNSM RDPVS
Sbjct: 1261 DNPHLRRVLMSPVRVHSFDSAIRMQERIRKGLDPSTFQLSTIRSFHASADYNSMPRDPVS 1320
Query: 1321 SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS 1380
SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS
Sbjct: 1321 SLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPTS 1380
Query: 1381 IIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG 1440
IIAYALNSREYDDWVANKFTDNEGGR+VPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG
Sbjct: 1381 IIAYALNSREYDDWVANKFTDNEGGRSVPRIIEDSRPSTLSAWQSFGALNLDYIHYGNYG 1440
Query: 1441 SEDPTTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD 1500
SED TTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD
Sbjct: 1441 SEDSTTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKGVD 1500
Query: 1501 FVRSLSRCRKWSAQGGKS------------------------------------------ 1560
FVRSLSRCRKWSAQGGKS
Sbjct: 1501 FVRSLSRCRKWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPQYFKYLSLALNS 1560
Query: 1561 ----------------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT 1620
GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT
Sbjct: 1561 GSPTCLAKILGIYQVTVKHLKGGKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNPDT 1620
Query: 1621 TGADKVLLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER 1677
TGADKVLLDQNLLEALRTK IFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER
Sbjct: 1621 TGADKVLLDQNLLEALRTKTIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDEER 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy TrEMBL
Match:
A0A5D3E079 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G00830 PE=4 SV=1)
HSP 1 Score: 2722 bits (7056), Expect = 0.0
Identity = 1407/1749 (80.45%), Postives = 1514/1749 (86.56%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVR WISWGP+SSV LSEEFV PN SYKMCCECD SFSK SL Y+CQ
Sbjct: 1 MGIPDSSLSDLIEKVRCWISWGPNSSVSLSEEFVLPNNSYKMCCECDTSFSKMSLRYICQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SC+QSSC CIRGYESYV+QSD MKVGNEAVK TK CKFCSD NLR EGG RHSEKV+ S
Sbjct: 61 SCNQSSCGKCIRGYESYVLQSDGMKVGNEAVKRTKLCKFCSDTNLRLEGGRRHSEKVHPS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPE PSPSY G KSP KT+S+PDDRL RYL+SQDCGYSPYAAS+ +LS FN HP
Sbjct: 121 VSPRYSPEPPSPSYDGADKSPMKTQSLPDDRLSRYLDSQDCGYSPYAASHRILSSFNAHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
PISV HSPNWSDEDD EDSGRHFFSPSSDYFRD+SDIESSS+STRNEFFSSHF SSPS
Sbjct: 181 FPISVRHSPNWSDEDDAEDSGRHFFSPSSDYFRDNSDIESSSVSTRNEFFSSHFGGSSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSD------DHKHPFSSVLPST 300
SPSR+DYT +KAD+VVQ++DPGQR+NLL QEAETILNRS+ D+K S+ LP+
Sbjct: 241 DSPSRNDYTFSKADYVVQTIDPGQRVNLLSQEAETILNRSERLTEGQDNKQACSADLPTI 300
Query: 301 QNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
QNQD E LDFENN IWFPPPPDDESDELENNFF+Y+DEDD+IGDSGAMFS S S S
Sbjct: 301 QNQDGKVERPLDFENNGLIWFPPPPDDESDELENNFFSYNDEDDNIGDSGAMFSRSSSFS 360
Query: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAA 420
+MFPAKQNLHEENKEPL+AMVQGHFRALVAQLLQGEDIKSGGD V+ W+DVITTIAWQAA
Sbjct: 361 NMFPAKQNLHEENKEPLKAMVQGHFRALVAQLLQGEDIKSGGDCVDVWLDVITTIAWQAA 420
Query: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLL 480
NFVRPDTSRGGSMDPGDY+KVKCVASGHP ESTLIKGVVCTKNIKHKRMTSQFKN RLLL
Sbjct: 421 NFVRPDTSRGGSMDPGDYVKVKCVASGHPHESTLIKGVVCTKNIKHKRMTSQFKNPRLLL 480
Query: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAK 540
LGGSLEYH+V++ LASFNTLLHQENDHLKTIISKIESLRPNVL+VEKSVSS A++YLLAK
Sbjct: 481 LGGSLEYHQVANQLASFNTLLHQENDHLKTIISKIESLRPNVLVVEKSVSSCAQDYLLAK 540
Query: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
EISLVLNVKRPLLERIARCTGASLTPSI+H+S +RLGHCELFRLERVTEE NQNKK+
Sbjct: 541 EISLVLNVKRPLLERIARCTGASLTPSIEHISTARLGHCELFRLERVTEELMDTNQNKKL 600
Query: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
SKTLMFFEGCPKRLGCTVLLKG CREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP
Sbjct: 601 SKTLMFFEGCPKRLGCTVLLKGRCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
Query: 661 KMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNF 720
KMP RS+SV+D+SSS NP+ + +SN LVD S S+L++ELEESK Y + HL N++F
Sbjct: 661 KMPTRSISVDDHSSSENPNASEASNFLVDGSARENFSALNSELEESKSYSVDSHLGNSSF 720
Query: 721 LLSTGFEEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
LLSTGFE SIVDT P IFND PSD+ L PSL+ EELKDG TVPF VRSLSESEL E
Sbjct: 721 LLSTGFEAS-SIVDTLPDIFNDDLPSDIDLKPSLNPCEELKDGITVPFAVRSLSESELPE 780
Query: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
TL+ + +SGGI E +KSESFDENDLSSE FSAADTHQSILVSFSSHCVLKGTVCERSRL
Sbjct: 781 TLKWKNGRSGGIPELRKSESFDENDLSSEYFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
Query: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIK 900
RIKFYGRFDKPLG++L DDLF Q SSC SCKESAEAHV CYTHQQGNLTINVRRLPSIK
Sbjct: 841 YRIKFYGRFDKPLGRYLRDDLFDQKSSCPSCKESAEAHVLCYTHQQGNLTINVRRLPSIK 900
Query: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
LPGEEDG+IWMWHRCLKCAQIDGVPPATPRV+MSDAAWGLSFGKFLELSFSNHATANRVA
Sbjct: 901 LPGEEDGKIWMWHRCLKCAQIDGVPPATPRVVMSDAAWGLSFGKFLELSFSNHATANRVA 960
Query: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIG 1020
TCGHSLQRDCLRFYG+GSMVAFFRYSTIDILSV LPPSVLEFNSHVQQEWIS EASEIIG
Sbjct: 961 TCGHSLQRDCLRFYGYGSMVAFFRYSTIDILSVNLPPSVLEFNSHVQQEWISNEASEIIG 1020
Query: 1021 KMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMM 1080
KMESLYAEISD+VDRMEQKSKL MQESSDSS++QSH+ QLKEL+QKERSDYIGLL+PFMM
Sbjct: 1021 KMESLYAEISDVVDRMEQKSKLPMQESSDSSDVQSHITQLKELLQKERSDYIGLLQPFMM 1080
Query: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
+PSL+GMISVDILELNRLRRSLL+HTHYWDHRLYSLDSLLKKI N K DVSS + R LK
Sbjct: 1081 KPSLMGMISVDILELNRLRRSLLMHTHYWDHRLYSLDSLLKKILNSKTDVSSSIMRDLKS 1140
Query: 1141 DSAQSDDKIEFGCNENIHESCLVHKSAGND-LQSMQKEEPTAVECSKSTLHQVCREEEHT 1200
DSA SD K+E G NENIHES L H+ A ND + S +KEE TA E S ST HQ CREE HT
Sbjct: 1141 DSAHSDGKLESGHNENIHESSLAHEFAENDDIHSDRKEESTAYERSTSTSHQACREETHT 1200
Query: 1201 DEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQ 1260
D E+T +A E IPSYESTLSERIDCAWSG+EQS+ K VS LQA+EP DGA KQMSQ
Sbjct: 1201 DGELTTSMA-FESIPSYESTLSERIDCAWSGTEQSMKKHPTVSALQAEEPNDGAVKQMSQ 1260
Query: 1261 NDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPV 1320
ND+PQ+RRVLMSPVRVHSFDSAIRMQERIRKGL+PSTFQLS+IRSFHASADY++MLRDP+
Sbjct: 1261 NDHPQMRRVLMSPVRVHSFDSAIRMQERIRKGLYPSTFQLSSIRSFHASADYSTMLRDPI 1320
Query: 1321 SSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPT 1380
S+LTR HSQ+LP E QK NLLLSST S+ISASHVPEGVRLLLPQTGN D VIAVYDNEPT
Sbjct: 1321 SNLTRTHSQMLPLEVQKSNLLLSSTTSYISASHVPEGVRLLLPQTGNNDIVIAVYDNEPT 1380
Query: 1381 SIIAYALNSREYDDWVANKFTDNEGGRNVPRI-IEDSRPSTLSAWQSFGALNLDYIHYGN 1440
SIIAYALNSREYDDWVA+KF+ NEGGR+VPRI IEDSRPS LSAWQSFG L+LDYIHYGN
Sbjct: 1381 SIIAYALNSREYDDWVADKFSVNEGGRSVPRINIEDSRPSPLSAWQSFGGLDLDYIHYGN 1440
Query: 1441 YGSEDPTTLRTLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPKG 1500
YGSED +T+RTLFSDPKKSPHLR+SF DESS SGGKV FSVTCYFAKQFD+LRKKCCPKG
Sbjct: 1441 YGSEDSSTMRTLFSDPKKSPHLRLSFVDESSISGGKVKFSVTCYFAKQFDLLRKKCCPKG 1500
Query: 1501 VDFVRSLSRCRKWSAQGGKS---------------------------------------- 1560
VDFVRSLSRC +WSAQGGKS
Sbjct: 1501 VDFVRSLSRCCRWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPEYFKYLSQAL 1560
Query: 1561 ------------------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYNP 1620
GKE+KMDLMVMENLFFMRNISKVYDLKGSSRSRYNP
Sbjct: 1561 TYGSPTCLAKILGIYQVTVKHLKGGKEMKMDLMVMENLFFMRNISKVYDLKGSSRSRYNP 1620
Query: 1621 DTTGADKVLLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVDE 1677
DT+GA+KVLLD NLLEALRTKPIFLGSKAKR LERAIWNDT FLASIDVMDYSLLVG+DE
Sbjct: 1621 DTSGANKVLLDLNLLEALRTKPIFLGSKAKRILERAIWNDTSFLASIDVMDYSLLVGIDE 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy TrEMBL
Match:
A0A1S3BYX5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103495094 PE=4 SV=1)
HSP 1 Score: 2706 bits (7014), Expect = 0.0
Identity = 1402/1750 (80.11%), Postives = 1509/1750 (86.23%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
MGIPDSSLSDLIEKVR WISWGP+SSV LSEEFV PN SYKMCCECD SFSK SL Y+CQ
Sbjct: 1 MGIPDSSLSDLIEKVRCWISWGPNSSVSLSEEFVLPNNSYKMCCECDTSFSKMSLRYICQ 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
SC+QSSC CIRGYESYV+QSD MKVGNEAVK TK CKFCSD NLR EGG RHSEKV+ S
Sbjct: 61 SCNQSSCGKCIRGYESYVLQSDGMKVGNEAVKRTKLCKFCSDTNLRLEGGRRHSEKVHPS 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLFNDHP 180
VSPRYSPE PSPSY G KSP KT+S+PDDRL RYL+SQDCGYSPYAAS+ +LS FN HP
Sbjct: 121 VSPRYSPEPPSPSYDGADKSPMKTQSLPDDRLSRYLDSQDCGYSPYAASHRILSSFNAHP 180
Query: 181 SPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEESSPS 240
PISV HSPNWSDEDD EDSGRHFFSPSSDYFRD+SDIESSS+STRNEFFSSHF SSPS
Sbjct: 181 FPISVRHSPNWSDEDDAEDSGRHFFSPSSDYFRDNSDIESSSVSTRNEFFSSHFGGSSPS 240
Query: 241 ASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSD------DHKHPFSSVLPST 300
SPSR+DYT +KAD+VVQ++DPGQR+NLL QEAETILNRS+ D+K S+ LP+
Sbjct: 241 DSPSRNDYTFSKADYVVQTIDPGQRVNLLSQEAETILNRSERLTEGQDNKQACSADLPTI 300
Query: 301 QNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASRSLS 360
QNQD E LDFENN IWFPPPPDDESDELENNFF+Y+DEDD+IGDSGAMFS S S S
Sbjct: 301 QNQDGKVERPLDFENNGLIWFPPPPDDESDELENNFFSYNDEDDNIGDSGAMFSRSSSFS 360
Query: 361 SMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGDSVEDWVDVITTIAWQAA 420
+MFPAKQNLHEENKEPL+AMVQGHFRALVAQLLQGEDIKSGGD V+ W+DVITTIAWQAA
Sbjct: 361 NMFPAKQNLHEENKEPLKAMVQGHFRALVAQLLQGEDIKSGGDCVDVWLDVITTIAWQAA 420
Query: 421 NFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNARLLL 480
NFVRPDTSRGGSMDPGDY+KVKCVASGHP ESTLIKGVVCTKNIKHKRMTSQFKN RLLL
Sbjct: 421 NFVRPDTSRGGSMDPGDYVKVKCVASGHPHESTLIKGVVCTKNIKHKRMTSQFKNPRLLL 480
Query: 481 LGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEYLLAK 540
LGGSLEYH+V++ LASFNTLLHQENDHLKTIISKIESLRPNVL+VEKSVSS A++YLLAK
Sbjct: 481 LGGSLEYHQVANQLASFNTLLHQENDHLKTIISKIESLRPNVLVVEKSVSSCAQDYLLAK 540
Query: 541 EISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQNKKM 600
EISLVLNVKRPLLERIARCTGASLTPSI+H+S +RLGHCELFRLERVTEE NQNKK+
Sbjct: 541 EISLVLNVKRPLLERIARCTGASLTPSIEHISTARLGHCELFRLERVTEELMDTNQNKKL 600
Query: 601 SKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
SKTLMFFEGCPKRLGCTVLLKG CREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP
Sbjct: 601 SKTLMFFEGCPKRLGCTVLLKGRCREELKKVKHVVQYAVFAAYHLSLETSFLVDEGASLP 660
Query: 661 KMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHHLDNNNF 720
KMP RS+SV+D+SSS NP+ + +SN LVD S S+L++ELEESK Y + HL N++F
Sbjct: 661 KMPTRSISVDDHSSSENPNASEASNFLVDGSARENFSALNSELEESKSYSVDSHLGNSSF 720
Query: 721 LLSTGFEEEPSIVDTFPGIFNDHSPSDVGLNPSLSHYEELKDGETVPFDVRSLSESELAE 780
LLSTGFE SIVDT P IFND PSD+ L PSL+ EELKDG TVPF VRSLSESEL E
Sbjct: 721 LLSTGFEAS-SIVDTLPDIFNDDLPSDIDLKPSLNPCEELKDGITVPFAVRSLSESELPE 780
Query: 781 TLERRREKSGGITEFQKSESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
TL+ + +SGGI E +KSESFDENDLSSE FSAADTHQSILVSFSSHCVLKGTVCERSRL
Sbjct: 781 TLKWKNGRSGGIPELRKSESFDENDLSSEYFSAADTHQSILVSFSSHCVLKGTVCERSRL 840
Query: 841 LRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIK 900
RIKFYGRFDKPLG++L DDLF Q SSC SCKESAEAHV CYTHQQGNLTINVRRLPSIK
Sbjct: 841 YRIKFYGRFDKPLGRYLRDDLFDQKSSCPSCKESAEAHVLCYTHQQGNLTINVRRLPSIK 900
Query: 901 LPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVA 960
LPGEEDG+IWMWHRCLKCAQIDGVPPATPRV+MSDAAWGLSFGKFLELSFSNHATANRVA
Sbjct: 901 LPGEEDGKIWMWHRCLKCAQIDGVPPATPRVVMSDAAWGLSFGKFLELSFSNHATANRVA 960
Query: 961 TCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIG 1020
TCGHSLQRDCLRFYG+GSMVAFFRYSTIDILSV LPPSVLEFNSHVQQEWIS EASEIIG
Sbjct: 961 TCGHSLQRDCLRFYGYGSMVAFFRYSTIDILSVNLPPSVLEFNSHVQQEWISNEASEIIG 1020
Query: 1021 KMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMM 1080
KMESLYAEISD+VDRMEQKSKL MQESSDSS++QSH+ QLKEL+QKERSDYIGLL+PFMM
Sbjct: 1021 KMESLYAEISDVVDRMEQKSKLPMQESSDSSDVQSHITQLKELLQKERSDYIGLLQPFMM 1080
Query: 1081 EPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLKR 1140
+PSL+GMISVDILELNRLRRSLL+HTHYWDHRLYSLDSLLKKI N K DVSS + R LK
Sbjct: 1081 KPSLMGMISVDILELNRLRRSLLMHTHYWDHRLYSLDSLLKKILNSKTDVSSSIMRDLKS 1140
Query: 1141 DSAQSDDKIEFGCNENIHESCLVHKSAGND-LQSMQKEEPTAVECSKSTLHQVCREEEHT 1200
DSA SD K+E G NENIHES L H+ A ND + S +KEE TA E S ST HQ CREE HT
Sbjct: 1141 DSAHSDGKLESGHNENIHESSLAHEFAENDDIHSDRKEESTAYERSTSTSHQACREETHT 1200
Query: 1201 DEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGATKQMSQ 1260
D E+T +A E IPSYESTLSERIDCAWSG+EQS+ K VS LQA+EP DGA KQMSQ
Sbjct: 1201 DGELTTSMA-FESIPSYESTLSERIDCAWSGTEQSMKKHPTVSALQAEEPNDGAVKQMSQ 1260
Query: 1261 NDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYNSMLRDPV 1320
ND+PQ+RRVLMSPVRVHSFDSAIRMQERIRKGL+PSTFQLS+IRSFHASADY++MLRDP+
Sbjct: 1261 NDHPQMRRVLMSPVRVHSFDSAIRMQERIRKGLYPSTFQLSSIRSFHASADYSTMLRDPI 1320
Query: 1321 SSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPEGVRLLLPQTGNIDAVIAVYDNEPT 1380
S+LTR HSQ+LP E QK NLLLSST S+ISASHVPEGVRLLLPQTGN D VIAVYDNEPT
Sbjct: 1321 SNLTRTHSQMLPLEVQKSNLLLSSTTSYISASHVPEGVRLLLPQTGNNDIVIAVYDNEPT 1380
Query: 1381 SIIAYALNSREYDDWVANKFTDNEGGRNVPRI-IEDSRPSTLSAWQSFGALNLDYIHYGN 1440
SIIAYALNSREYDDWVA+KF+ NEGGR+VPRI IEDSRPS LSAWQSFG L+LDYIHYGN
Sbjct: 1381 SIIAYALNSREYDDWVADKFSVNEGGRSVPRINIEDSRPSPLSAWQSFGGLDLDYIHYGN 1440
Query: 1441 YGSEDPTTLR-TLFSDPKKSPHLRISFTDESSTSGGKVNFSVTCYFAKQFDILRKKCCPK 1500
YGSED +T+ F PKKSPHLR+SF DESS SGGKV FSVTCYFAKQFD+LRKKCCPK
Sbjct: 1441 YGSEDSSTMXGPCFQIPKKSPHLRLSFVDESSISGGKVKFSVTCYFAKQFDLLRKKCCPK 1500
Query: 1501 GVDFVRSLSRCRKWSAQGGKS--------------------------------------- 1560
GVDFVRSLSRC +WSAQGGKS
Sbjct: 1501 GVDFVRSLSRCCRWSAQGGKSNVYFAKSLDERFIIKQVTRTELDSFEEFAPEYFKYLSQA 1560
Query: 1561 -------------------------GKEVKMDLMVMENLFFMRNISKVYDLKGSSRSRYN 1620
GKE+KMDLMVMENLFFMRNISKVYDLKGSSRSRYN
Sbjct: 1561 LTYGSPTCLAKILGIYQVTVKHLKGGKEMKMDLMVMENLFFMRNISKVYDLKGSSRSRYN 1620
Query: 1621 PDTTGADKVLLDQNLLEALRTKPIFLGSKAKRNLERAIWNDTYFLASIDVMDYSLLVGVD 1677
PDT+GA+KVLLD NLLEALRTKPIFLGSKAKR LERAIWNDT FLASIDVMDYSLLVG+D
Sbjct: 1621 PDTSGANKVLLDLNLLEALRTKPIFLGSKAKRILERAIWNDTSFLASIDVMDYSLLVGID 1680
BLAST of Cp4.1LG01g06240 vs. ExPASy TrEMBL
Match:
A0A6J1KKC6 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111494775 PE=4 SV=1)
HSP 1 Score: 2671 bits (6924), Expect = 0.0
Identity = 1376/1717 (80.14%), Postives = 1484/1717 (86.43%), Query Frame = 0
Query: 32 EFVAPNKSYKMCCECDMSFSKTSLVYLCQSCSQSSCRNCIRGYESYVVQSDVMKVGNEAV 91
EFV PN S+KMCCECD SFS+ SL YLCQSC+QSSC C+RGYES+VVQSD MKVGNEA+
Sbjct: 2 EFVMPNNSFKMCCECDTSFSQMSLRYLCQSCNQSSCGKCVRGYESFVVQSDGMKVGNEAM 61
Query: 92 KTTKSCKFCSDGNLRHEGGGRHSEKVYSSVSPRYSPEAPSPSYRGTYKSPKKTESIPDDR 151
K K CKFCSD NLR EGG R SEKVY SVSPRYSPE PSPSY G KSP K ES+ DDR
Sbjct: 62 KRIKLCKFCSDDNLRREGGRRQSEKVYPSVSPRYSPEPPSPSYDGADKSPMKIESLSDDR 121
Query: 152 LCRYLESQDCGYSPYAASNGLLSLFNDHPSPISVHHSPNWSDEDDGEDSGRHFFSPSSDY 211
L RYL SQDCGYSPYAAS+ + S FN HP PISV HSPNWSDEDD EDSGRH+FSPSSDY
Sbjct: 122 LSRYLGSQDCGYSPYAASHRISSSFNAHPFPISVRHSPNWSDEDDAEDSGRHYFSPSSDY 181
Query: 212 FRDSSDIESSSISTRNEFFSSHFEESSPSASPSRSDYTSNKADHVVQSVDPGQRLNLLPQ 271
RDSSDIESSSISTRNEFFSSHF ESSPS SPSR+DYT +KAD+VVQ++DPGQR NL Q
Sbjct: 182 CRDSSDIESSSISTRNEFFSSHFRESSPSDSPSRNDYTLSKADYVVQTIDPGQRENLSSQ 241
Query: 272 EAETILNRSD------DHKHPFSSVLPSTQNQDENAETLLDFENNDRIWFPPPPDDESDE 331
+AE ILNRS+ ++KH S LP+ Q+Q+ E LDFENN IWFPPPPDDE D+
Sbjct: 242 DAEAILNRSERLTDGQNNKHACSGDLPTIQDQEGKVERPLDFENNGLIWFPPPPDDEIDD 301
Query: 332 LENNFFAYDDEDDDIGDSGAMFSASRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQ 391
LENNFFAYDDEDDDIGDSGAMFS S SLSSMFPAKQNL E NKEPLRAMVQGHFRALVAQ
Sbjct: 302 LENNFFAYDDEDDDIGDSGAMFSRSSSLSSMFPAKQNLQEVNKEPLRAMVQGHFRALVAQ 361
Query: 392 LLQGEDIKSGGDSVEDWVDVITTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRE 451
LLQG+DIKSGGD VEDW+DVITTIAWQAANFVRPDTSRGGSMDPGDY+KVKCVASG P E
Sbjct: 362 LLQGDDIKSGGDYVEDWLDVITTIAWQAANFVRPDTSRGGSMDPGDYVKVKCVASGRPHE 421
Query: 452 STLIKGVVCTKNIKHKRMTSQFKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTI 511
STLIKGVVCTKNIKHKRMTSQFKN RLLLLGGSLEYH+V++ LASFNTLL+QENDHLKTI
Sbjct: 422 STLIKGVVCTKNIKHKRMTSQFKNPRLLLLGGSLEYHQVANQLASFNTLLYQENDHLKTI 481
Query: 512 ISKIESLRPNVLLVEKSVSSRAKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHM 571
ISKIESLRPNVLLVEKSVSS A++YLLAKEISLVLNVK+PLLERIARCTGASLTPSIDH+
Sbjct: 482 ISKIESLRPNVLLVEKSVSSCAQDYLLAKEISLVLNVKKPLLERIARCTGASLTPSIDHI 541
Query: 572 SISRLGHCELFRLERVTEEYGTVNQNKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKV 631
S++RLGHCELFRLERVTEE+G NQNKK+SKTLMFFEGCPKRLGCTVLLKGMCREELKKV
Sbjct: 542 SMARLGHCELFRLERVTEEHGDANQNKKLSKTLMFFEGCPKRLGCTVLLKGMCREELKKV 601
Query: 632 KHVVQYAVFAAYHLSLETSFLVDEGASLPKMPKRSLSVEDNSSSGNPDVTMSSNCLVDVS 691
K VVQYA+FAAYHLSLETSFLVDEGASLPKMPKRS+SVEDNSSS NP+ +SNCL D+S
Sbjct: 602 KDVVQYAIFAAYHLSLETSFLVDEGASLPKMPKRSISVEDNSSSENPNAPEASNCLFDLS 661
Query: 692 TGYKVSSLSAELEESKIYPMYHHLDNNNFLLSTGFEEEPSIVDTFPGIFNDHSPSDVGLN 751
T K S+LS+EL+ESKIY M HL +N+FL ST FE+ SIVDT P IFND PS+V L
Sbjct: 662 TEDKFSALSSELDESKIYSMDSHLGDNSFLQSTDFEDS-SIVDTLPDIFNDDLPSEVDLK 721
Query: 752 PSLSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGITEFQKSESFDENDLSSECF 811
PSL+ EE KDG TVPF RSLSESEL ETL+ R KSGGI E +KSESFDE +LS+E F
Sbjct: 722 PSLNRCEEFKDGLTVPFATRSLSESELPETLKWRMGKSGGIPELRKSESFDEKELSNEYF 781
Query: 812 SAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLCDDLFAQTSSCRSC 871
SAADTHQSILVSFSSHCVLKGT+CERSRLLRIKFYGRFDKPLG++L DDLF QTSSC SC
Sbjct: 782 SAADTHQSILVSFSSHCVLKGTICERSRLLRIKFYGRFDKPLGRYLRDDLFDQTSSCPSC 841
Query: 872 KESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKCAQIDGVPPATPRV 931
KESAEAHV CYTHQQGNLTINVRRLPSIKLPGEEDG+IWMWHRCLKCAQIDGVPPATPRV
Sbjct: 842 KESAEAHVLCYTHQQGNLTINVRRLPSIKLPGEEDGKIWMWHRCLKCAQIDGVPPATPRV 901
Query: 932 IMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGSMVAFFRYSTIDIL 991
+MSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYG+GSMVAFFRYSTIDIL
Sbjct: 902 VMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGYGSMVAFFRYSTIDIL 961
Query: 992 SVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQKSKLSMQESSDSS 1051
SV LPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDI+DRMEQK+KLS Q+SSDSS
Sbjct: 962 SVNLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIIDRMEQKNKLSTQDSSDSS 1021
Query: 1052 EMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRLRRSLLIHTHYWDH 1111
++QSH+ QLKEL+QKERSDYIGLL+PFMM+PSL+GMISVDILELNRLRRSLL+HTH WDH
Sbjct: 1022 DVQSHIAQLKELLQKERSDYIGLLQPFMMKPSLMGMISVDILELNRLRRSLLMHTHSWDH 1081
Query: 1112 RLYSLDSLLKKISNPKADVSSPLKRVLKRDSAQSDDKIEFGCNENIHESCLVHKSAGNDL 1171
RLYSLDSLLKKI NPK D SSP+ R K DSA SD K+EFG NENIHES +H+ AGNDL
Sbjct: 1082 RLYSLDSLLKKILNPKTDASSPVMRDSKSDSAHSDGKLEFGHNENIHESSSIHEFAGNDL 1141
Query: 1172 QSMQKEEPTAVECSKSTLHQVCREEEHTDEEMTAVIASIEGIPSYESTLSERIDCAWSGS 1231
S QKEE TA ECS ST HQ CREE HTD E++ +A + I SYESTLSERIDCAWSG+
Sbjct: 1142 HSEQKEESTAFECSASTSHQACREEAHTDGELSTSMAFL-SISSYESTLSERIDCAWSGT 1201
Query: 1232 EQSVTKPQAVSTLQAQEPPDGATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKG 1291
EQSV KP AVS LQ +EP DG KQ+SQ DNPQ++RVLMSPVRVHSFDSAIRMQERIRKG
Sbjct: 1202 EQSVKKPPAVSALQTEEPLDGGVKQVSQKDNPQMKRVLMSPVRVHSFDSAIRMQERIRKG 1261
Query: 1292 LHPSTFQLSTIRSFHASADYNSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISAS 1351
LHPST QLS IRSFHASADY+SMLRDPVSSLTRAHSQ+LP+ETQKLNLLLSSTPSFIS S
Sbjct: 1262 LHPSTCQLSNIRSFHASADYSSMLRDPVSSLTRAHSQMLPWETQKLNLLLSSTPSFISTS 1321
Query: 1352 HVPEGVRLLLPQTGNIDAVIAVYDNEPTSIIAYALNSREYDDWVANKFTDNEGGRNVPRI 1411
H+ EGVRLLLPQTGN D VIAVYDNEPTSIIAYALNSREYDDWVA+KFT+N+GG+++PRI
Sbjct: 1322 HILEGVRLLLPQTGNNDIVIAVYDNEPTSIIAYALNSREYDDWVADKFTNNDGGQSIPRI 1381
Query: 1412 -IEDSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDPKKSPHLRISFTDESST 1471
IEDSRP+TLSAWQSFGAL+LDYIHYGNYGSED +T+RTLFSDPKKSPHLR+SF DESST
Sbjct: 1382 NIEDSRPATLSAWQSFGALDLDYIHYGNYGSEDSSTMRTLFSDPKKSPHLRLSFVDESST 1441
Query: 1472 SGGKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS------------ 1531
SGGKV FSVTCYFAKQFD+LRKKCCPKGVDFVRSLSRC +WSAQGGKS
Sbjct: 1442 SGGKVKFSVTCYFAKQFDLLRKKCCPKGVDFVRSLSRCCRWSAQGGKSNVYFAKSLDERF 1501
Query: 1532 ----------------------------------------------------GKEVKMDL 1591
GKE+KMDL
Sbjct: 1502 IIKQVTRTELDSFEEFAPEYFKYLSHSLTSGSPTCLAKILGIYQVTVKHLKGGKEMKMDL 1561
Query: 1592 MVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKVLLDQNLLEALRTKPIFLGSKAKRN 1651
MVMENLFFMR+ISKVYDLKGSSRSRYNPDT+GA+KVLLD NLLEALRTKPIFLGSKAKR
Sbjct: 1562 MVMENLFFMRSISKVYDLKGSSRSRYNPDTSGANKVLLDLNLLEALRTKPIFLGSKAKRK 1621
Query: 1652 LERAIWNDTYFLASIDVMDYSLLVGVDEERKELVLGIIDFMRQYTWDKQLETWVKASGIL 1677
LERAIWNDT FLASIDVMDYSLLVGVDEERKELVLGIIDFMRQYTWDKQLETWVKASGIL
Sbjct: 1622 LERAIWNDTSFLASIDVMDYSLLVGVDEERKELVLGIIDFMRQYTWDKQLETWVKASGIL 1681
BLAST of Cp4.1LG01g06240 vs. TAIR 10
Match:
AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )
HSP 1 Score: 1544.3 bits (3997), Expect = 0.0e+00
Identity = 902/1778 (50.73%), Postives = 1128/1778 (63.44%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCL----SEEFVAPNKSYKMCCECDMSFSKTSLV 60
MGIPD SL DLI+KVRSWI+ S+ L ++F KMC +C +K
Sbjct: 1 MGIPDGSLLDLIDKVRSWITSDSSDSLFLLSSSKQDFGIMPIVSKMCHDCG---TKVEQG 60
Query: 61 YLCQSCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEK 120
Y C SC C++C ES + K C+ C D +R K
Sbjct: 61 YCCLSCGSCWCKSCSDTEESKM----------------KLCREC-DAEVR-----ELRVK 120
Query: 121 VYSSVSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAASNGLLSLF 180
Y V PR SP+ P S TES + L LE +DC
Sbjct: 121 SYDKVHPRDSPDPP---------SSLATES---ESLASSLEIRDC--------------- 180
Query: 181 NDHPSPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSISTRNEFFSSHFEE 240
+ S+ P+ +E++ G+ SPSSD ++DSSDIES S+S R+E FS
Sbjct: 181 ---RNMASIRCYPSRGEEEEARYCGKQLLSPSSDNYQDSSDIESGSVSARHELFSCKSSA 240
Query: 241 -SSPSASPSRSDYT--SNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSVLPS 300
SSP SP R++++ H P + E +L +D+ P VL
Sbjct: 241 GSSPHDSPLRNNFSPLGRFVQHAKDLRSP--TVCSFDNHQEQLL--ADNLVKPGQGVLEQ 300
Query: 301 TQNQDE--NAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSASR 360
+++E + LDFENN RIW+PPPP+DE+D+ E+N+F YDDEDDDIGDS FS S
Sbjct: 301 EDHEEEEDKLQQPLDFENNGRIWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSS 360
Query: 361 SLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIKSGGD-SVEDWVDVITTIA 420
S SS P K+ L E + EPLR +V HFRALVA+LL+GE++ D S +W+D++T +A
Sbjct: 361 SFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALA 420
Query: 421 WQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQFKNA 480
WQAANFV+PDT GGSMDPG+Y+K+KCVASG+ ES LI+G+VC+KNI HKRM SQ+KN
Sbjct: 421 WQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNP 480
Query: 481 RLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRAKEY 540
R++LL GSLEY V+ LASFNTLL QEN+H+K II+KIESLRPNVLLVEKS SS A++Y
Sbjct: 481 RVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQY 540
Query: 541 LLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGTVNQ 600
LL KEISLVLNVKR LL+RIARCTGA L PS+D +S +RLGHCELFR ERV E++ NQ
Sbjct: 541 LLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQ 600
Query: 601 -NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFLVDE 660
N+K S+TLM+FEGCP+RLGCTV+L+G CREELKKVKHV+QYAVFAAYHLSLETSFL DE
Sbjct: 601 SNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADE 660
Query: 661 GASLPKMP-KRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSSLSAELEESKIYPMYHH 720
GASLPK+ K+ V S D +S T + +A E+ PM H
Sbjct: 661 GASLPKIRLKQPGMVRTASQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPEH 720
Query: 721 LDNNNF--------LLSTGFEEEPSIVDTFPGIFNDH----SPSDVGLN----PSLSHYE 780
+ + E E DT G F ++ S S LN P+L
Sbjct: 721 EVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSSNQLNDLHEPTLCLSS 780
Query: 781 ELKDGETVPFDVRSLSESELAETLERRREKSGGIT---EFQKSESFDENDLSSECFSAAD 840
E+ + T + E R E+ + + + ESF E+D+SSE FSAAD
Sbjct: 781 EIPETPT---------QQPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDVSSEYFSAAD 840
Query: 841 THQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESA 900
+HQSILVSFSS CVLK +VCERSRLLRIKFYG FDKPLG++L DDLF +TSSCRSCKE
Sbjct: 841 SHQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELV 900
Query: 901 EAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSD 960
+AHV CY+HQ GNLTINVRRLPS+KLPGE+DG+IWMWHRCL+CA +DGVPPAT RV+MSD
Sbjct: 901 DAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSD 960
Query: 961 AAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTL 1020
AAWGLSFGKFLELSFSNHATANRVA+CGHSLQRDCLRFYGFG+MVAFFRYS I+IL+V L
Sbjct: 961 AAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLL 1020
Query: 1021 PPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQS 1080
PPS+LEFNSH QQEWI EA+E++GKM ++Y EISD+++RME+KS L E S++ ++ S
Sbjct: 1021 PPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEPEQSEACDLHS 1080
Query: 1081 HVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYS 1140
++ L + + KE+ +Y L+P + E +L S+DILELNRLRR+L+I H WDH+LY
Sbjct: 1081 RIIGLIDQLVKEKDEYDDALQP-IFEENLQIQGSLDILELNRLRRALMIGAHAWDHQLYL 1140
Query: 1141 LDSLLKKISNPKADVSSPLKRVLKRDSAQSDDKIEFGCNENIHESCLVHKSAGNDLQSMQ 1200
L+S LKK S K + + D + D +++ G +E
Sbjct: 1141 LNSQLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDER------------------- 1200
Query: 1201 KEEPTAVECSKSTLHQVCREEEHTDEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSV 1260
E+ HTD E E IPS ++LSERID AW GS Q++
Sbjct: 1201 ------------------DEQSHTDSEANGDNKDPENIPSPGTSLSERIDSAWLGSFQNL 1260
Query: 1261 TKPQAVSTLQAQEPPDGATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPS 1320
K + ++ + N LRR L P+RV SFDSAIR QERI+KGL PS
Sbjct: 1261 EKAETIAETEGFSAV-----------NSSLRR-LARPIRVQSFDSAIRFQERIQKGLPPS 1320
Query: 1321 TFQLSTIRSFHASADYNSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFI-SASHVP 1380
+ LST+RSFHAS +Y +M+RDPVS++ R +SQ+LP E QKL+L++ S P++I SAS +
Sbjct: 1321 SLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLIVGSAPTYISSASQMA 1380
Query: 1381 EGVRLLLPQTGNIDAVIAVYDNEPTSIIAYALNSREYDDWVANK-FTDNEGGRNVPRIIE 1440
+G R+L+PQ G D V+ VYD++P S+++YA+NS+EY +W+ NK + N+
Sbjct: 1381 DGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEYKEWIVNKGLASSSSSSNLNN--R 1440
Query: 1441 DSRPSTLSAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDPKKSPHLRISFTDESSTSG- 1500
+S PS S W+S ++++DYI + YGS D +KSPHL ISF+D +S+S
Sbjct: 1441 ESEPSAFSTWRSL-SMDVDYIQHAVYGSS---------QDDRKSPHLTISFSDRASSSST 1500
Query: 1501 ---GKVNFSVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS----------- 1560
GKV FSVTCYFA QFD LRK CCP VDFVRSLSRC++WSAQGGKS
Sbjct: 1501 ATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQGGKSNVYFAKSLDER 1560
Query: 1561 -----------------------------------------------------GKEVKMD 1620
GKE KMD
Sbjct: 1561 FIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQVSIKHPKGGKETKMD 1620
Query: 1621 LMVMENLFFMRNISKVYDLKGSSRSRYNPDTTGADKVLLDQNLLEALRTKPIFLGSKAKR 1678
LMVMENLF+ R IS++YDLKGS+RSRYNP+T+GADKVLLD NLLE LRT+PIFLGSKAKR
Sbjct: 1621 LMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLETLRTEPIFLGSKAKR 1648
BLAST of Cp4.1LG01g06240 vs. TAIR 10
Match:
AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )
HSP 1 Score: 1048.5 bits (2710), Expect = 5.7e-306
Identity = 690/1828 (37.75%), Postives = 995/1828 (54.43%), Query Frame = 0
Query: 1 MGIPDS---SLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVY 60
MG DS + S+++ ++SW+ W + + +S +F P++S ++C ECD F+ + +
Sbjct: 1 MGTRDSNNRTFSEIVGLIKSWLPWRSEPAT-VSRDFWMPDQSCRVCYECDCQFTLINRRH 60
Query: 61 LCQSCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKV 120
C+ C + C C S ++ E + + C +C + +GG S
Sbjct: 61 HCRHCGRVFCGKCTA--NSIPFAPSDLRTPREDWERIRVCNYCFRQWEQGDGGPHVSNIT 120
Query: 121 YSSVSPRYSPEAPSPSYRGTYKSPKKTESIP------------DDRLCRYLESQDCGYSP 180
S SP + S + S S+P D + S + +
Sbjct: 121 ELSTSPSETSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTK 180
Query: 181 YAASNGLLSLFNDHPSPISVHHSPNWSDEDDGEDSGRHFFSPSSDYFRDSSDIESSSIST 240
S F + N DD D +Y +DIE+S
Sbjct: 181 QGKETSRRSSFIATDVEDPSRFALNSIRSDDEYD----------EYGAYQTDIETSHSPR 240
Query: 241 RNEFFSSHFEESSPSASPSRSDYTSNKADHVVQSVDP--GQRLNLLPQEAETILNRSDDH 300
NE++ AD S P Q L L +E + +H
Sbjct: 241 ANEYYGPMEYNGMGIDDVPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEH 300
Query: 301 --KHPFSSVLPSTQNQDENAETLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIG 360
+ + P+ + D+ E +DFENN +W PP P++E DE E+ F DE+D+ G
Sbjct: 301 DGRDECEASSPADISDDQVVEP-VDFENNGLLWVPPEPENEEDERESALF---DEEDNEG 360
Query: 361 DSGAMFSASRSLSSM----FPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK-SGG 420
D+ + R +S + + EE+K+ ++ +V GHFRAL+AQLLQ E+I S
Sbjct: 361 DASGEWGYLRPSTSFGSGEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDE 420
Query: 421 DSVEDWVDVITTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTK 480
+ E W+++IT+++W+AAN ++PD S+ G MDPG Y+KVKC+ASG +S ++KGVVC K
Sbjct: 421 EGKESWLEIITSLSWEAANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKK 480
Query: 481 NIKHKRMTSQFKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNV 540
N+ ++RM+++ + ARLL+LGG LEY VS+ L+SF+TLL QE DHLK ++KI + RPN+
Sbjct: 481 NVVNRRMSTKIEKARLLILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNI 540
Query: 541 LLVEKSVSSRAKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELF 600
LLVEKSVS A+EYLLAK+ISLVLN+KRPLL+RIARCTGA + PS+DH+S +LG+CE F
Sbjct: 541 LLVEKSVSRFAQEYLLAKDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENF 600
Query: 601 RLERVTEEYGTVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFA 660
R++R EE+G+ Q KK+ KTLM+FE CPK LG T+LL+G +ELKKVKHVVQY VFA
Sbjct: 601 RVDRYPEEHGSTGQVGKKVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFA 660
Query: 661 AYHLSLETSFLVDEGASLPKMPKRS---LSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS 720
AYHL+LETSFL DEGAS P++P S +++ D S+S ++ V
Sbjct: 661 AYHLALETSFLADEGAS-PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTML 720
Query: 721 LSAELEESKIYPMYHHLDNNNFL----------LSTGFEEEPSIVDTFPGIFNDHSPSDV 780
AE + + P+ L L +G++ I +F +F+ H+ S
Sbjct: 721 SCAEPQRANSVPVSELLSTTTNLSIQKDIPPIPYGSGWQAR-EINPSF--VFSRHNISLN 780
Query: 781 GLNPSLSHYEELKDGETVPFDVRS-------------------------LSESELAETLE 840
+ + G +VP D + S++ ++
Sbjct: 781 LPDRVIESRNSDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIM 840
Query: 841 RRREKSGG---ITEFQKSESFDENDLSSECFSAADT-HQSILVSFSSHCVLKGTVCERSR 900
+ +G I + Q +E E E F + + HQSILVS SS V KGTVCERS
Sbjct: 841 VENQDNGSELTIAQQQNNEKPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSH 900
Query: 901 LLRIKFYGRFDKPLGKFLCDDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSI 960
L RIK+YG FDKPLG+FL D LF Q+ CRSC+ +EAHV CYTH+QG+LTI+V++L
Sbjct: 901 LFRIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDY 960
Query: 961 KLPGEEDGRIWMWHRCLKCAQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRV 1020
LPGE++G+IWMWHRCL+C +++G PPAT RV+MSDAAWGLSFGKFLELSFSNHA A+RV
Sbjct: 961 LLPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRV 1020
Query: 1021 ATCGHSLQRDCLRFYGFGSMVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEII 1080
A CGHSL RDCLRFYGFG+MVA FRY+TID+ SV LPPS+L FN + Q+WI +E E+I
Sbjct: 1021 ACCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFN-YENQDWIQRETDEVI 1080
Query: 1081 GKMESLYAEISDIVDRMEQKSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFM 1140
+ E L++E+ + + ++ +K + + +L+E++QKE++++ ++ +
Sbjct: 1081 ERAELLFSEVLNAISQIAEKG------------FRRRIGELEEVLQKEKAEFEENMQKIL 1140
Query: 1141 MEPSLLGMISVDILELNRLRRSLLIHTHYWDHRLYSLDSLLKKISNPKADVSSPLKRVLK 1200
G VDILEL R+ R LL ++ WDHRL + +L K ++ K L
Sbjct: 1141 HREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKREENEKPPLA 1200
Query: 1201 RDSAQSDDKIEFGCNENIHESCLVHKSAGN------DLQSMQKEEPTAVECSKSTLHQVC 1260
+ +Q+ ++ G N + S + G+ L ++QKE T S L+Q
Sbjct: 1201 K--SQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADT-----NSDLYQ-- 1260
Query: 1261 REEEHTDEEMTAVIASIEGIPSYESTLSERIDCAWSGSEQSVTKPQAVSTLQAQEPPDGA 1320
+++ ++ + +P L ++D + S+ + +TL A +
Sbjct: 1261 ------EKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQ 1320
Query: 1321 TK-QMSQNDNPQLRRVLMS------PVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFH 1380
T ++ N+ L MS P+ + Q+ K +P + L + +++
Sbjct: 1321 TSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPS-KNYE 1380
Query: 1381 ASADYNSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQ 1440
S D S L P + R+ ++ +QKL+ +P +IS+ E G RLLLP
Sbjct: 1381 NSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLP- 1440
Query: 1441 TGNIDAVIAVYDNEPTSIIAYALNSREYDDWVA---NKFTDNEGGRNVPRIIEDSRPSTL 1500
G D V+ VYD+EPTS+IAYAL S EY + N+PR ++D +
Sbjct: 1441 VGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDD---TIF 1500
Query: 1501 SAWQSFGALNLDYIHYGNYGSEDPTTLRTLFSDP---KKSPHLRISFTDESSTSGGKVNF 1560
+S G+++ + + S T DP K+ H R+S+ ++ + GKV +
Sbjct: 1501 DPSRSNGSVDESILSISSSRS-------TSLLDPLSYTKALHARVSYGEDGTL--GKVKY 1560
Query: 1561 SVTCYFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGG--------------------- 1620
+VTCY+AK+F+ LR C P ++++RSLSRC+KW AQGG
Sbjct: 1561 TVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTK 1620
Query: 1621 -------------------------------------------KSGKEVKMDLMVMENLF 1676
KSGKE KMD+++MENL
Sbjct: 1621 TELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLL 1680
BLAST of Cp4.1LG01g06240 vs. TAIR 10
Match:
AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1040.8 bits (2690), Expect = 1.2e-303
Identity = 704/1823 (38.62%), Postives = 1002/1823 (54.96%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
M D ++ V+SWI +SS +S +F P++S +C ECD F+ + + C+
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPRKSESS-NMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
C + C C S SD K +E + + C +C + E G + S
Sbjct: 61 LCGRVFCAKC--AANSIPSPSDETKDSHEEPERIRVCNYCYK---QWEQGIVPPDNGASI 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAA---SNGLLSLFN 180
+S +S SPS R + + + D S +P A+ S+ + S +
Sbjct: 121 ISLHFS---SSPSARSVASTTSNSSNCTIDSTAG--PSPRPKMNPRASRRVSSNMDSEKS 180
Query: 181 DHPSPISVHHSPNWSDEDDGEDSGRHFF--SPSSDYFRDSSDIESS----SISTRNEFFS 240
+ + S S ++ D D+ FF S SD D D S S + N+++
Sbjct: 181 EQQNAKSRRSSDHYGHVLDSSDNQVEFFVNSGRSDGEADDDDDYQSDFAQSYAQGNDYYG 240
Query: 241 S-HFEESSPSASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSSV 300
+ + +E + K + + P Q L+ L E ++++ ++ ++ V
Sbjct: 241 AINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTET---IDKTRQQENGWNDV 300
Query: 301 LPSTQNQDENAE-TLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFSA 360
+ +E+ E ++DFE++ +W PP P++E DE E D ++ D GD G + +
Sbjct: 301 KEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPS 360
Query: 361 SRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK--SGGDSVEDWVDVIT 420
+ F +K +++ ++ +V+GHFRALVAQLL+ +++ + GD E W+D+IT
Sbjct: 361 NSFNEKDFHSK----DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDE-EGWLDIIT 420
Query: 421 TIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQF 480
+++W+AA ++PDTS+ G MDPG Y+KVKC+ G ES ++KGVVC KN+ H+RMTS+
Sbjct: 421 SLSWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKI 480
Query: 481 KNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSRA 540
+ RLL+LGG+LEY +S+ L+SF+TLL QE DHLK ++KI+S P++LLVEKSVS A
Sbjct: 481 EKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFA 540
Query: 541 KEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYGT 600
+EYLLAK+ISLVLN+KR LLERI+RCTGA + PSID ++ +LG+C+LF +E+ E + +
Sbjct: 541 QEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS 600
Query: 601 VNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSFL 660
Q KKM+KTLMFF+GCPK LGCT+LLKG +ELKKVKHV+QY VFAAYHL+LETSFL
Sbjct: 601 PCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFL 660
Query: 661 VDEGASLPKMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS-------------- 720
DEGAS+ ++P ++ + PD N + G+ VSS
Sbjct: 661 ADEGASIHELP-----LQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPH 720
Query: 721 -----LSAELEESKIYPMYHHLDNNNFL---------LSTGFEEEPSIVDTFPGIFNDHS 780
L+ SK + LD N+ + L T + + P + +
Sbjct: 721 KANGDLTGNFTSSKTH-FQGKLDGNDRIDPSERLLHNLDTVYCKPPETI----------T 780
Query: 781 PSDVGLNPS-----LSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGITEFQKSE 840
D GL P+ LS + E + + V S + ++ + G F + E
Sbjct: 781 SKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQE 840
Query: 841 SFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLCD 900
+ + + +A HQSILVS S+ CV KG+VCER+ LLRIK+YG FDKPLG+FL D
Sbjct: 841 QMESS--KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRD 900
Query: 901 DLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKCA 960
+LF Q C SC AEAH+ CYTH+QG+LTI+V++LP + LPG+ +G+IWMWHRCLKC
Sbjct: 901 NLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKCP 960
Query: 961 QIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGSM 1020
+I+G PPAT R++MSDAAWGLSFGKFLELSFSNHA A+RVA CGHSL RDCLRFYGFG M
Sbjct: 961 RINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRM 1020
Query: 1021 VAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQK 1080
VA FRY++I+I +VTLPP+ L FN + QEW+ KE+ E+I K E L+ E+ + + ++ K
Sbjct: 1021 VACFRYASINIYAVTLPPAKLYFN-YENQEWLQKESKEVIKKAEVLFNEVQEALSQISAK 1080
Query: 1081 SKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRLR 1140
+ + + S ++++ + +L L+++ + +Y L+ M+ G ++DIL +N+LR
Sbjct: 1081 TMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQ-MLNVVKDGQPTIDILLINKLR 1140
Query: 1141 RSLLIHTHYWDHRLYSLDSLLKK--ISNPKADVSSPLKRVLKRDSAQSDDKIE------F 1200
R ++ ++ WD L ++++ + PK + R + + SD+K++
Sbjct: 1141 RLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKL-SDEKVKSIPTHVA 1200
Query: 1201 GCNENI-----HESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDE----- 1260
CN+++ +E+CL + D + E S CR E E
Sbjct: 1201 ICNDSLLQDADYETCLNQGKSFADTSG----KFAIPEDVGSDRPPDCRMEFDPSEGGKDN 1260
Query: 1261 --EMTAVIASIEGIPSYEST-LSERIDCAWSG----SEQSVTKP--QAVSTLQAQEPPDG 1320
E + V+ +++T LS+ +D AW G SE + +P +A ST Q P
Sbjct: 1261 FVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP--- 1320
Query: 1321 ATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADYN 1380
+R+ +S + + H + QL + SF+ S + N
Sbjct: 1321 -------------------DLRLLGSESELNFKGGPTNDEHTTQVQLPS-PSFYYSLNKN 1380
Query: 1381 SMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQTGNIDA 1440
L +R H ++ P ++S+ E G RLLLP G D
Sbjct: 1381 YSLN------SRKH------------IMAEDRPVYVSSYRELEWRSGARLLLP-LGCNDL 1440
Query: 1441 VIAVYDNEPTSIIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGAL 1500
V+ VYD+EPTSIIAYAL S EY ++ G + R DS S S + S L
Sbjct: 1441 VLPVYDDEPTSIIAYALTSSEYKAQMS--------GSDKSRDRLDSGGS-FSLFDSVNLL 1500
Query: 1501 NLDYIH------YGNYGSEDPTTLRTLFSD-PKKSPHLRISFTDESSTSGGKVNFSVTCY 1560
+L+ + + S D + L S K H RISFTDE GKV +SVTCY
Sbjct: 1501 SLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPP--GKVKYSVTCY 1560
Query: 1561 FAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS------------------------ 1620
+AK+F+ LR CCP DF+RSL RCRKW AQGGKS
Sbjct: 1561 YAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELES 1620
Query: 1621 ----------------------------------------GKEVKMDLMVMENLFFMRNI 1676
GKE KMD++VMENL F RN
Sbjct: 1621 FIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNF 1680
BLAST of Cp4.1LG01g06240 vs. TAIR 10
Match:
AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1040.4 bits (2689), Expect = 1.6e-303
Identity = 704/1824 (38.60%), Postives = 1002/1824 (54.93%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
M D ++ V+SWI +SS +S +F P++S +C ECD F+ + + C+
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPRKSESS-NMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
C + C C S SD K +E + + C +C + E G + S
Sbjct: 61 LCGRVFCAKC--AANSIPSPSDETKDSHEEPERIRVCNYCYK---QWEQGIVPPDNGASI 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAA---SNGLLSLFN 180
+S +S SPS R + + + D S +P A+ S+ + S +
Sbjct: 121 ISLHFS---SSPSARSVASTTSNSSNCTIDSTAG--PSPRPKMNPRASRRVSSNMDSEKS 180
Query: 181 DHPSPISVHHSPNWSDEDDGEDSGRHFF---SPSSDYFRDSSDIESS----SISTRNEFF 240
+ + S S ++ D D+ FF S SD D D S S + N+++
Sbjct: 181 EQQNAKSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQSYAQGNDYY 240
Query: 241 SS-HFEESSPSASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSS 300
+ + +E + K + + P Q L+ L E ++++ ++ ++
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTET---IDKTRQQENGWND 300
Query: 301 VLPSTQNQDENAE-TLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFS 360
V + +E+ E ++DFE++ +W PP P++E DE E D ++ D GD G +
Sbjct: 301 VKEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRP 360
Query: 361 ASRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK--SGGDSVEDWVDVI 420
++ F +K +++ ++ +V+GHFRALVAQLL+ +++ + GD E W+D+I
Sbjct: 361 SNSFNEKDFHSK----DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDE-EGWLDII 420
Query: 421 TTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQ 480
T+++W+AA ++PDTS+ G MDPG Y+KVKC+ G ES ++KGVVC KN+ H+RMTS+
Sbjct: 421 TSLSWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSK 480
Query: 481 FKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSR 540
+ RLL+LGG+LEY +S+ L+SF+TLL QE DHLK ++KI+S P++LLVEKSVS
Sbjct: 481 IEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRF 540
Query: 541 AKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYG 600
A+EYLLAK+ISLVLN+KR LLERI+RCTGA + PSID ++ +LG+C+LF +E+ E +
Sbjct: 541 AQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHV 600
Query: 601 TVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSF 660
+ Q KKM+KTLMFF+GCPK LGCT+LLKG +ELKKVKHV+QY VFAAYHL+LETSF
Sbjct: 601 SPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSF 660
Query: 661 LVDEGASLPKMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS------------- 720
L DEGAS+ ++P ++ + PD N + G+ VSS
Sbjct: 661 LADEGASIHELP-----LQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEP 720
Query: 721 ------LSAELEESKIYPMYHHLDNNNFL---------LSTGFEEEPSIVDTFPGIFNDH 780
L+ SK + LD N+ + L T + + P +
Sbjct: 721 HKANGDLTGNFTSSKTH-FQGKLDGNDRIDPSERLLHNLDTVYCKPPETI---------- 780
Query: 781 SPSDVGLNPS-----LSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGITEFQKS 840
+ D GL P+ LS + E + + V S + ++ + G F +
Sbjct: 781 TSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQ 840
Query: 841 ESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLC 900
E + + + +A HQSILVS S+ CV KG+VCER+ LLRIK+YG FDKPLG+FL
Sbjct: 841 EQMESS--KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLR 900
Query: 901 DDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKC 960
D+LF Q C SC AEAH+ CYTH+QG+LTI+V++LP + LPG+ +G+IWMWHRCLKC
Sbjct: 901 DNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKC 960
Query: 961 AQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGS 1020
+I+G PPAT R++MSDAAWGLSFGKFLELSFSNHA A+RVA CGHSL RDCLRFYGFG
Sbjct: 961 PRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGR 1020
Query: 1021 MVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQ 1080
MVA FRY++I+I +VTLPP+ L FN + QEW+ KE+ E+I K E L+ E+ + + ++
Sbjct: 1021 MVACFRYASINIYAVTLPPAKLYFN-YENQEWLQKESKEVIKKAEVLFNEVQEALSQISA 1080
Query: 1081 KSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRL 1140
K+ + + S ++++ + +L L+++ + +Y L+ M+ G ++DIL +N+L
Sbjct: 1081 KTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQ-MLNVVKDGQPTIDILLINKL 1140
Query: 1141 RRSLLIHTHYWDHRLYSLDSLLKK--ISNPKADVSSPLKRVLKRDSAQSDDKIE------ 1200
RR ++ ++ WD L ++++ + PK + R + + SD+K++
Sbjct: 1141 RRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKL-SDEKVKSIPTHV 1200
Query: 1201 FGCNENI-----HESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDE---- 1260
CN+++ +E+CL + D + E S CR E E
Sbjct: 1201 AICNDSLLQDADYETCLNQGKSFADTSG----KFAIPEDVGSDRPPDCRMEFDPSEGGKD 1260
Query: 1261 ---EMTAVIASIEGIPSYEST-LSERIDCAWSG----SEQSVTKP--QAVSTLQAQEPPD 1320
E + V+ +++T LS+ +D AW G SE + +P +A ST Q P
Sbjct: 1261 NFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP-- 1320
Query: 1321 GATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADY 1380
+R+ +S + + H + QL + SF+ S +
Sbjct: 1321 --------------------DLRLLGSESELNFKGGPTNDEHTTQVQLPS-PSFYYSLNK 1380
Query: 1381 NSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQTGNID 1440
N L +R H ++ P ++S+ E G RLLLP G D
Sbjct: 1381 NYSLN------SRKH------------IMAEDRPVYVSSYRELEWRSGARLLLP-LGCND 1440
Query: 1441 AVIAVYDNEPTSIIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGA 1500
V+ VYD+EPTSIIAYAL S EY ++ G + R DS S S + S
Sbjct: 1441 LVLPVYDDEPTSIIAYALTSSEYKAQMS--------GSDKSRDRLDSGGS-FSLFDSVNL 1500
Query: 1501 LNLDYIH------YGNYGSEDPTTLRTLFSD-PKKSPHLRISFTDESSTSGGKVNFSVTC 1560
L+L+ + + S D + L S K H RISFTDE GKV +SVTC
Sbjct: 1501 LSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPP--GKVKYSVTC 1560
Query: 1561 YFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS----------------------- 1620
Y+AK+F+ LR CCP DF+RSL RCRKW AQGGKS
Sbjct: 1561 YYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELE 1620
Query: 1621 -----------------------------------------GKEVKMDLMVMENLFFMRN 1676
GKE KMD++VMENL F RN
Sbjct: 1621 SFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRN 1680
BLAST of Cp4.1LG01g06240 vs. TAIR 10
Match:
AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )
HSP 1 Score: 1038.9 bits (2685), Expect = 4.5e-303
Identity = 703/1824 (38.54%), Postives = 1000/1824 (54.82%), Query Frame = 0
Query: 1 MGIPDSSLSDLIEKVRSWISWGPDSSVCLSEEFVAPNKSYKMCCECDMSFSKTSLVYLCQ 60
M D ++ V+SWI +SS +S +F P++S +C ECD F+ + + C+
Sbjct: 1 MDSQDHKAPGFVDIVKSWIPRKSESS-NMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCR 60
Query: 61 SCSQSSCRNCIRGYESYVVQSDVMKVGNEAVKTTKSCKFCSDGNLRHEGGGRHSEKVYSS 120
C + C C S SD K +E + + C +C + E G + S
Sbjct: 61 LCGRVFCAKC--AANSIPSPSDETKDSHEEPERIRVCNYCYK---QWEQGIVPPDNGASI 120
Query: 121 VSPRYSPEAPSPSYRGTYKSPKKTESIPDDRLCRYLESQDCGYSPYAA---SNGLLSLFN 180
+S +S SPS R + + + D S +P A+ S+ + S +
Sbjct: 121 ISLHFS---SSPSARSVASTTSNSSNCTIDSTAG--PSPRPKMNPRASRRVSSNMDSEKS 180
Query: 181 DHPSPISVHHSPNWSDEDDGEDSGRHFF---SPSSDYFRDSSDIESS----SISTRNEFF 240
+ + S S ++ D D+ FF S SD D D S S + N+++
Sbjct: 181 EQQNAKSRRSSDHYGHVLDSSDNQVEFFVNSSGRSDGEADDDDDYQSDFAQSYAQGNDYY 240
Query: 241 SS-HFEESSPSASPSRSDYTSNKADHVVQSVDPGQRLNLLPQEAETILNRSDDHKHPFSS 300
+ + +E + K + + P Q L+ L E ++++ ++ ++
Sbjct: 241 GAINLDEVDHIYGSHEAHDVGVKIEPNISGFPPDQDLDSLNTET---IDKTRQQENGWND 300
Query: 301 VLPSTQNQDENAE-TLLDFENNDRIWFPPPPDDESDELENNFFAYDDEDDDIGDSGAMFS 360
V + +E+ E ++DFE++ +W PP P++E DE E D ++ D GD G +
Sbjct: 301 VKEGSPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRP 360
Query: 361 ASRSLSSMFPAKQNLHEENKEPLRAMVQGHFRALVAQLLQGEDIK--SGGDSVEDWVDVI 420
++ F +K +++ ++ +V+GHFRALVAQLL+ +++ + GD E W+D+I
Sbjct: 361 SNSFNEKDFHSK----DKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDE-EGWLDII 420
Query: 421 TTIAWQAANFVRPDTSRGGSMDPGDYIKVKCVASGHPRESTLIKGVVCTKNIKHKRMTSQ 480
T+++W+AA ++PDTS+ G MDPG Y+KVKC+ G ES ++KGVVC KN+ H+RMTS+
Sbjct: 421 TSLSWEAATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSK 480
Query: 481 FKNARLLLLGGSLEYHEVSDHLASFNTLLHQENDHLKTIISKIESLRPNVLLVEKSVSSR 540
+ RLL+LGG+LEY +S+ L+SF+TLL QE DHLK ++KI+S P++LLVEKSVS
Sbjct: 481 IEKPRLLILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRF 540
Query: 541 AKEYLLAKEISLVLNVKRPLLERIARCTGASLTPSIDHMSISRLGHCELFRLERVTEEYG 600
A+EYLLAK+ISLVLN+KR LLERI+RCTGA + PSID ++ +LG+C+LF +E+ E +
Sbjct: 541 AQEYLLAKDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHV 600
Query: 601 TVNQ-NKKMSKTLMFFEGCPKRLGCTVLLKGMCREELKKVKHVVQYAVFAAYHLSLETSF 660
+ Q KKM+KTLMFF+GCPK LGCT+LLKG +ELKKVKHV+QY VFAAYHL+LETSF
Sbjct: 601 SPCQVAKKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSF 660
Query: 661 LVDEGASLPKMPKRSLSVEDNSSSGNPDVTMSSNCLVDVSTGYKVSS------------- 720
L DEGAS+ ++P ++ + PD N + G+ VSS
Sbjct: 661 LADEGASIHELP-----LQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEP 720
Query: 721 ------LSAELEESKIYPMYHHLDNNNFL---------LSTGFEEEPSIVDTFPGIFNDH 780
L+ SK + LD N+ + L T + + P +
Sbjct: 721 HKANGDLTGNFTSSKTH-FQGKLDGNDRIDPSERLLHNLDTVYCKPPETI---------- 780
Query: 781 SPSDVGLNPS-----LSHYEELKDGETVPFDVRSLSESELAETLERRREKSGGITEFQKS 840
+ D GL P+ LS + E + + V S + ++ + G F +
Sbjct: 781 TSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGNQNFNRQ 840
Query: 841 ESFDENDLSSECFSAADTHQSILVSFSSHCVLKGTVCERSRLLRIKFYGRFDKPLGKFLC 900
E + + + +A HQSILVS S+ CV KG+VCER+ LLRIK+YG FDKPLG+FL
Sbjct: 841 EQMESS--KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLR 900
Query: 901 DDLFAQTSSCRSCKESAEAHVQCYTHQQGNLTINVRRLPSIKLPGEEDGRIWMWHRCLKC 960
D+LF Q C SC AEAH+ CYTH+QG+LTI+V++LP + LPG+ +G+IWMWHRCLKC
Sbjct: 901 DNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKLPEL-LPGQREGKIWMWHRCLKC 960
Query: 961 AQIDGVPPATPRVIMSDAAWGLSFGKFLELSFSNHATANRVATCGHSLQRDCLRFYGFGS 1020
+I+G PPAT R++MSDAAWGLSFGKFLELSFSNHA A+RVA CGHSL RDCLRFYGFG
Sbjct: 961 PRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGR 1020
Query: 1021 MVAFFRYSTIDILSVTLPPSVLEFNSHVQQEWISKEASEIIGKMESLYAEISDIVDRMEQ 1080
MVA FRY++I+I +VTLPP+ L FN + QEW+ KE+ E+I K E L+ E+ + + ++
Sbjct: 1021 MVACFRYASINIYAVTLPPAKLYFN-YENQEWLQKESKEVIKKAEVLFNEVQEALSQISA 1080
Query: 1081 KSKLSMQESSDSSEMQSHVMQLKELIQKERSDYIGLLKPFMMEPSLLGMISVDILELNRL 1140
K+ + + S ++++ + +L L+++ + +Y +L G ++DIL +N+L
Sbjct: 1081 KTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKQMLNVVKD-----GQPTIDILLINKL 1140
Query: 1141 RRSLLIHTHYWDHRLYSLDSLLKK--ISNPKADVSSPLKRVLKRDSAQSDDKIE------ 1200
RR ++ ++ WD L ++++ + PK + R + + SD+K++
Sbjct: 1141 RRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKL-SDEKVKSIPTHV 1200
Query: 1201 FGCNENI-----HESCLVHKSAGNDLQSMQKEEPTAVECSKSTLHQVCREEEHTDE---- 1260
CN+++ +E+CL + D + E S CR E E
Sbjct: 1201 AICNDSLLQDADYETCLNQGKSFADTSG----KFAIPEDVGSDRPPDCRMEFDPSEGGKD 1260
Query: 1261 ---EMTAVIASIEGIPSYEST-LSERIDCAWSG----SEQSVTKP--QAVSTLQAQEPPD 1320
E + V+ +++T LS+ +D AW G SE + +P +A ST Q P
Sbjct: 1261 NFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIP-- 1320
Query: 1321 GATKQMSQNDNPQLRRVLMSPVRVHSFDSAIRMQERIRKGLHPSTFQLSTIRSFHASADY 1380
+R+ +S + + H + QL + SF+ S +
Sbjct: 1321 --------------------DLRLLGSESELNFKGGPTNDEHTTQVQLPS-PSFYYSLNK 1380
Query: 1381 NSMLRDPVSSLTRAHSQVLPFETQKLNLLLSSTPSFISASHVPE---GVRLLLPQTGNID 1440
N L +R H ++ P ++S+ E G RLLLP G D
Sbjct: 1381 NYSLN------SRKH------------IMAEDRPVYVSSYRELEWRSGARLLLP-LGCND 1440
Query: 1441 AVIAVYDNEPTSIIAYALNSREYDDWVANKFTDNEGGRNVPRIIEDSRPSTLSAWQSFGA 1500
V+ VYD+EPTSIIAYAL S EY ++ G + R DS S S + S
Sbjct: 1441 LVLPVYDDEPTSIIAYALTSSEYKAQMS--------GSDKSRDRLDSGGS-FSLFDSVNL 1500
Query: 1501 LNLDYIH------YGNYGSEDPTTLRTLFSD-PKKSPHLRISFTDESSTSGGKVNFSVTC 1560
L+L+ + + S D + L S K H RISFTDE GKV +SVTC
Sbjct: 1501 LSLNSLSDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEGPP--GKVKYSVTC 1560
Query: 1561 YFAKQFDILRKKCCPKGVDFVRSLSRCRKWSAQGGKS----------------------- 1620
Y+AK+F+ LR CCP DF+RSL RCRKW AQGGKS
Sbjct: 1561 YYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELE 1620
Query: 1621 -----------------------------------------GKEVKMDLMVMENLFFMRN 1676
GKE KMD++VMENL F RN
Sbjct: 1621 SFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRN 1680
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9SSJ8 | 0.0e+00 | 50.73 | Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thalia... | [more] |
Q9LUM0 | 8.0e-305 | 37.75 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=370... | [more] |
Q0WUR5 | 2.2e-302 | 38.60 | 1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=370... | [more] |
Q9XID0 | 9.8e-178 | 31.56 | Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thalia... | [more] |
P34756 | 3.6e-95 | 23.61 | 1-phosphatidylinositol 3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae (st... | [more] |
Match Name | E-value | Identity | Description | |
XP_023514916.1 | 0.0 | 96.32 | putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita pepo subsp... | [more] |
KAG7030579.1 | 0.0 | 95.17 | putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C, partial [Cucurbita a... | [more] |
KAG6599898.1 | 0.0 | 94.77 | putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C, partial [Cucurbita a... | [more] |
XP_022941900.1 | 0.0 | 94.60 | putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita moschata] | [more] |
XP_022990385.1 | 0.0 | 93.70 | putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FV39 | 0.0 | 94.60 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC... | [more] |
A0A6J1JPY8 | 0.0 | 93.70 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
A0A5D3E079 | 0.0 | 80.45 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=11946... | [more] |
A0A1S3BYX5 | 0.0 | 80.11 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103495... | [more] |
A0A6J1KKC6 | 0.0 | 80.14 | 1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC11... | [more] |
Match Name | E-value | Identity | Description | |
AT1G71010.1 | 0.0e+00 | 50.73 | FORMS APLOID AND BINUCLEATE CELLS 1C | [more] |
AT3G14270.1 | 5.7e-306 | 37.75 | phosphatidylinositol-4-phosphate 5-kinase family protein | [more] |
AT4G33240.2 | 1.2e-303 | 38.62 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |
AT4G33240.1 | 1.6e-303 | 38.60 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |
AT4G33240.3 | 4.5e-303 | 38.54 | 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinos... | [more] |