Cp4.1LG01g06130 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g06130
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCRAL-TRIO domain-containing protein
LocationCp4.1LG01: 393279 .. 396483 (+)
RNA-Seq ExpressionCp4.1LG01g06130
SyntenyCp4.1LG01g06130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATAGACCAGGGACCATAGCTACGACTGCACCAGAAACTCTCCTCCTCACCTCTCTTCCAACTCATTTATTCCCTCTCTCACACTCTCTGCATTCTCCTATCAGTTCGTCATCAATTCGGTTCCACGCCTGATCTCATCACCTAACCTCTGCCCGTCGCCGACGTCGATTCACCTTACCTCCACGGATTCCGAGATGGAGGATTCTCGCGCCAATGCGCTAATCCAGATGAGGAAAGCCGTCGAGAAATCAGGCATCAACACACAAGTAACAAGAAATCAAAATCCTGATCTATTCGTTTTTTCGTTCGATTAGCTGATGGATTTCTGACTCTGTGAGTGTTTCGACAGGAGTACTGCGATGCGACGATGATGAGATTCTTGATCGCGAGGTCTATGGAGCCTTCGAAAGGAGCGAAGATGTTCGTACAATGGCGGAAATGGAGAGATGCGGTGGTTCCGAAGGGTTTTATTTGTGAATCGGAGGTGAAGGAGGAGTTGGAGGCTAAGAAGATTTTCTTGCAGGGATTGTCCAAGACAGGACTGGCTGTGATGATTGTTATGGCTAAACGCCATTTTTCTTCCAAGGATCAACTCCAGTTCAAGAGTTAGTTTTCTGATTTCGTTCTTCTTCTTCTTCTTTTCTTCTAATTGTAATTTTGAAATTATTTGGAGTTCTTTTCTTCAAGTTTAAACAAAAATAGATTGTGAATGTTTTGATTATTTGTTTGCCTGAATCTAATTCACACTCCTCTCTCTGTTCTATGCAGAATTCCTGGTTCATCTCTTGGACAAGGTCATTGCAAGGTGACCTACTCAATTTTAATCATGAATAAAAAATGCATTTTAAAAATATATCGTTATTTTCATCTTTTTACAAATTATTTATATAGGTTTTTAAACATTAACTACTTCTAGATCATTTATACAGGATTTTAAATATATCATTAACTACTTCTAGATAATTGAATCTAATTTTTTTTAGTTGTTTTTTGAAATAATTTTTTAATTAGCTAAATTAATAAAAATACCCCCATAATTCTCTAACCATTAAAGTTTAAAAGTTTCATTCATGCCTCAACTTTTATTCTTGTCAGTATATGGTAATCCTCTGTAACCGTGCATAAATTATTTAAAACTTTTTAATATTAGTTTTGGAAATTTATTAGTATTTTTAAAATGTGATACCGAAGTAATAATACTTTTGAAAATTTTCAAAAAAAATTTAAAGATGTTTTTAAAGTTTTTAAAAAAGTTTAAAGATATTAATGAAAGTTTTGGGAATTGAAACAAAACAATTTTGAAACTTAAAGGTATTTTTTAAACATATTTATAAATTTAAGGGTACTCTTCAACCATTTCTATCCAAAAAAAAAAAAATTTAACTTCTTTCAAAGTTTAATGAATTTTTTAGATTTTTTTTTTGTATTTGATGGGTATTATTAAAATTTTTAAAATTTAGAAGTATCTTACTAAAAAAATTAAAGAATATTTTTATTAATGTAGTTTCTTTTGGAAAACAAATGGATTTTGAAAGTTAAGATAAAATAAATGAAATAAAAAATATTAAGTGGAAAAATCTGATTTGCATATGTGGACAGTGGTAGCAAAGGAAAAGAAATTGGAAACGAAAAGTGGATTGCTATTGTTGATCTTCACCAAATCTCATACAAGAATGTTGATATACGTGGACTCATCACAGGGTTTCAATTTCTACAGGTAAATCATATATTCCGTAATATCTTTATACCCTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAATTCAAGTCCTTGTGATTTTATAGTACATTGAAACGTTTTAGTGTGCATATAAAAAAAATGTTGCAGAATTACTACCCAGAACGCTTGGGTAAATGCTTCATTTTAAACATGCCATGGTTTTTCGTTAGCATTTGGAGAATGGTTTCTCGTTTCGTGGATCCTACAACGCTAAAAAACGTAAGCCCTTCCCTTCCCTTCCTTCTTTTCATTTCCAATATTAATTTCTTATTGTAGGAGAGAAGAAAGTAATGTTAGAAATCATGACTTTAAAGATTGTATGACATTATCCACTTTAAGCATAAACTTTCCTCAGATATATTTCTTATTTATAAACTTGGGATCATTTATTTAATTAACCAATATAACTCCTTAAAAATTGTGAAAAAATTTAATGAAAATGGACCCGTTTGATTTATGGGGTATTTGAAGTTTTGGGTGGGTGTTACGTTTTTGAGCGTTGGTTGGTTGGTTGTTACCTCTTCCTTTCTTACAAAACCTCCATAGGTATTAAGCTGCCACTTAGCTCCAAGTTTGTTAAGACAACAACACGTATAAAGGACCAGACAAAATTATTATAATACTATGCTAATTTAAAAATACATTTTTATTTTGATTCAGAAACTAAAATAAAGCATTAAATTTGTTTGACTACATAAAATAAGTATGTTTAAAATTTAAAGTAGAATCCCCCCATCTTTTATAGTTAAAAAATAGGCAAAAGTTGAACAAAAGAATAAAATGTCATGAATAGAATATAGAATATATATACAAAAAATTAAAATCTTACATTTAATCTTATACGTAACATTATTTATATATATATATATGCATGCATAAACGATGATTTTCAGATTTCAATTGTGAGCAATGAAGAAGAAAGAAGAAGTATGATAGAGGAAGTTGGAGAAGAAGTATTGCCAGAAGAGTACGGTGGAAAGGCAAAGTTTATTGCTCTTCAAGATGCTGTTCTTCCTCACTTGGATGCCTGAATCATTAAAACCAAGCAACTTAAATTTTTAGATTTATTGAGAAGATGCTCAAGATATTCCAAATAGTAATTTCATTTGAATGTAATTAAAGCCCAAATAATAGAGAGAGGGTTGTGCAAAATTTTAAGTCTTCCATAATGTTAAAGTTAGGTAAAGATTCTCTAGCTTAGCATCCATATTTACTTGAGCGGACCCATTGAGTACGGATAAGTCTATCTTGCCTGGCCTACAACAAAACATGATTGTGACTTTTAATTTGTCGACTATTTGATATAAAGATAATCGGTCTATCCCTATAAAGATGCAAAATAAATGCAAATTGAAGAGCTAATAGAGCCTGGTGGCTAGTGTTTCTAGAAAATTTAAGATCTCCGATCATGGTATTGAAGTGTGAAATAAAACTAGACATAAAACTTTAGAACT

mRNA sequence

CAAAATAGACCAGGGACCATAGCTACGACTGCACCAGAAACTCTCCTCCTCACCTCTCTTCCAACTCATTTATTCCCTCTCTCACACTCTCTGCATTCTCCTATCAGTTCGTCATCAATTCGGTTCCACGCCTGATCTCATCACCTAACCTCTGCCCGTCGCCGACGTCGATTCACCTTACCTCCACGGATTCCGAGATGGAGGATTCTCGCGCCAATGCGCTAATCCAGATGAGGAAAGCCGTCGAGAAATCAGGCATCAACACACAAGAGTACTGCGATGCGACGATGATGAGATTCTTGATCGCGAGGTCTATGGAGCCTTCGAAAGGAGCGAAGATGTTCGTACAATGGCGGAAATGGAGAGATGCGGTGGTTCCGAAGGGTTTTATTTGTGAATCGGAGGTGAAGGAGGAGTTGGAGGCTAAGAAGATTTTCTTGCAGGGATTGTCCAAGACAGGACTGGCTGTGATGATTGTTATGGCTAAACGCCATTTTTCTTCCAAGGATCAACTCCAGTTCAAGAAATTCCTGGTTCATCTCTTGGACAAGGTCATTGCAAGTGGTAGCAAAGGAAAAGAAATTGGAAACGAAAAGTGGATTGCTATTGTTGATCTTCACCAAATCTCATACAAGAATGTTGATATACGTGGACTCATCACAGGGTTTCAATTTCTACAGAATTACTACCCAGAACGCTTGGGTAAATGCTTCATTTTAAACATGCCATGGTTTTTCGTTAGCATTTGGAGAATGGTTTCTCGTTTCGTGGATCCTACAACGCTAAAAAACATTTCAATTGTGAGCAATGAAGAAGAAAGAAGAAGTATGATAGAGGAAGTTGGAGAAGAAGTATTGCCAGAAGAGTACGGTGGAAAGGCAAAGTTTATTGCTCTTCAAGATGCTGTTCTTCCTCACTTGGATGCCTGAATCATTAAAACCAAGCAACTTAAATTTTTAGATTTATTGAGAAGATGCTCAAGATATTCCAAATAGTAATTTCATTTGAATGTAATTAAAGCCCAAATAATAGAGAGAGGGTTGTGCAAAATTTTAAGTCTTCCATAATGTTAAAGTTAGGTAAAGATTCTCTAGCTTAGCATCCATATTTACTTGAGCGGACCCATTGAGTACGGATAAGTCTATCTTGCCTGGCCTACAACAAAACATGATTGTGACTTTTAATTTGTCGACTATTTGATATAAAGATAATCGGTCTATCCCTATAAAGATGCAAAATAAATGCAAATTGAAGAGCTAATAGAGCCTGGTGGCTAGTGTTTCTAGAAAATTTAAGATCTCCGATCATGGTATTGAAGTGTGAAATAAAACTAGACATAAAACTTTAGAACT

Coding sequence (CDS)

ATGGAGGATTCTCGCGCCAATGCGCTAATCCAGATGAGGAAAGCCGTCGAGAAATCAGGCATCAACACACAAGAGTACTGCGATGCGACGATGATGAGATTCTTGATCGCGAGGTCTATGGAGCCTTCGAAAGGAGCGAAGATGTTCGTACAATGGCGGAAATGGAGAGATGCGGTGGTTCCGAAGGGTTTTATTTGTGAATCGGAGGTGAAGGAGGAGTTGGAGGCTAAGAAGATTTTCTTGCAGGGATTGTCCAAGACAGGACTGGCTGTGATGATTGTTATGGCTAAACGCCATTTTTCTTCCAAGGATCAACTCCAGTTCAAGAAATTCCTGGTTCATCTCTTGGACAAGGTCATTGCAAGTGGTAGCAAAGGAAAAGAAATTGGAAACGAAAAGTGGATTGCTATTGTTGATCTTCACCAAATCTCATACAAGAATGTTGATATACGTGGACTCATCACAGGGTTTCAATTTCTACAGAATTACTACCCAGAACGCTTGGGTAAATGCTTCATTTTAAACATGCCATGGTTTTTCGTTAGCATTTGGAGAATGGTTTCTCGTTTCGTGGATCCTACAACGCTAAAAAACATTTCAATTGTGAGCAATGAAGAAGAAAGAAGAAGTATGATAGAGGAAGTTGGAGAAGAAGTATTGCCAGAAGAGTACGGTGGAAAGGCAAAGTTTATTGCTCTTCAAGATGCTGTTCTTCCTCACTTGGATGCCTGA

Protein sequence

MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPHLDA
Homology
BLAST of Cp4.1LG01g06130 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 77.4 bits (189), Expect = 2.5e-13
Identity = 63/247 (25.51%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           ++ ++  AL ++RK +E +G   +   D+T++RFL AR  +     +MF    KWR    
Sbjct: 29  LDSAQEKALAELRKLLEDAGF-IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYG 88

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTG----------LAVMIVMAKRHFSSKDQLQFKK 120
               + +    E+    K + Q   KT           L  + +      +S++++   K
Sbjct: 89  TDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERM--LK 148

Query: 121 FLVHLLDKVI------ASGSKGKEIGNEKWIAIVDLHQISYKNV-DIRGLITGFQFL-QN 180
            LV   + V+       S + G  +  E    I+DL  IS  +   +   +    ++ QN
Sbjct: 149 NLVWEYESVVQYRLPACSRAAGHLV--ETSCTIMDLKGISISSAYSVMSYVREASYISQN 208

Query: 181 YYPERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPE 230
           YYPER+GK +I+N P+ F + +R+   F+DP T+  I I+ +  + + +++++  E LP 
Sbjct: 209 YYPERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQ-KELLKQIPAENLPV 268

BLAST of Cp4.1LG01g06130 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 9.4e-13
Identity = 63/239 (26.36%), Postives = 116/239 (48.54%), Query Frame = 0

Query: 8   ALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFI-- 67
           AL+Q R  + +     +   D+T++RFL AR  + +   +MFV+  +WR+       I  
Sbjct: 32  ALLQFRSILLEKNYK-ERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTIIED 91

Query: 68  ----CESEVKEELEAKKIFLQ---GLSKTGLAVMI-----VMAKRHFSSKDQLQFKKFLV 127
                E+E KE ++  K++ Q    + K G  +       +  K+ +    + Q  + LV
Sbjct: 92  YENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLV 151

Query: 128 HLLDKV----IASGSKGKEIGNEKWIAIVDLHQISYKNV-DIRGLITGF-QFLQNYYPER 187
              +      + + S+      E    ++DL  IS  N   +   I       QNYYPER
Sbjct: 152 KEYELFATYRVPACSRRAGYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYYPER 211

Query: 188 LGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGG 227
           +GK +I++ P+ F ++++MV  F+DP T+  I I+ +   ++ +++++  E LP +YGG
Sbjct: 212 MGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGS-SYKKELLKQIPIENLPVKYGG 268

BLAST of Cp4.1LG01g06130 vs. ExPASy Swiss-Prot
Match: P53989 (SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=SEC14 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-12
Identity = 59/244 (24.18%), Postives = 118/244 (48.36%), Query Frame = 0

Query: 2   EDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVP 61
           ++++  AL Q+R  +E +G   +   D+T++RFL AR  + +   +MF    KWR     
Sbjct: 28  DEAQEGALKQLRSELEAAGFK-ERLDDSTLLRFLRARKFDVALAKEMFENCEKWRKEYGT 87

Query: 62  KGFICESEVKEELEAKKIFLQGLSKTG----------LAVMIVMAKRHFSSKDQLQFKKF 121
              + +    E+    K + Q   KT           L  + +      ++++++   K 
Sbjct: 88  NTIMQDFHYDEKPLVAKYYPQYYHKTDKDGRPVYFEELGAVNLTEMEKITTQERM--LKN 147

Query: 122 LVHLLDKVI----ASGSKGKEIGNEKWIAIVDLHQISYKNV-DIRGLITGFQFL-QNYYP 181
           LV   + V+     + S+      E    ++DL  IS  +   +   +    ++ QNYYP
Sbjct: 148 LVWEYESVVNYRLPACSRAAGYLVETSCTVMDLKGISISSAYSVLSYVREASYISQNYYP 207

Query: 182 ERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYG 230
           ER+GK +++N P+ F + +R+   F+DP T+  I I+ +  +   +++++  E LP ++G
Sbjct: 208 ERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQ-SELLKQIPAENLPSKFG 267

BLAST of Cp4.1LG01g06130 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-12
Identity = 61/251 (24.30%), Postives = 116/251 (46.22%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWR---- 60
           +  ++   L  MR  ++K G  T+   DAT++RFL AR     +  +MF++  KWR    
Sbjct: 24  LNSTQQATLDSMRLELQKLGY-TERLDDATLLRFLRARKFNLQQSLEMFIKCEKWRKEFG 83

Query: 61  -DAVVPKGFICESEV----------KEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQ 120
            D ++      E E           K +++ + ++++ L    L  +  +          
Sbjct: 84  VDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGNIDLKKLYQITTPE-RMMQN 143

Query: 121 LQFKKFLVHLLDKVIASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFL----- 180
           L ++  ++ L      S   G  I  E    I+DL     K V I  + + + ++     
Sbjct: 144 LVYEYEMLALKRFPACSRKAGGLI--ETSCTIMDL-----KGVGITSIHSVYSYIRQASS 203

Query: 181 --QNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEE 230
             Q+YYPER+GK +++N PW F S + ++  F+D  T+K I I+ +   + +++E++  +
Sbjct: 204 ISQDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGS-NYKSALLEQIPAD 263

BLAST of Cp4.1LG01g06130 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 2.1e-12
Identity = 57/239 (23.85%), Postives = 116/239 (48.54%), Query Frame = 0

Query: 8   ALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWR----------- 67
           AL ++RK ++++G  T+   D+T++RFL AR  + +    MF    KWR           
Sbjct: 34  ALEELRKVLKQAGF-TKRLDDSTLLRFLRARKFDVAAARAMFENCEKWRKENGVDTIFED 93

Query: 68  ----DAVVPKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQL-QFKKF 127
               +  +   F  +   K + + + ++++ L    L  M  +  +    K+ + +++ F
Sbjct: 94  FHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNLIWEYESF 153

Query: 128 LVHLLDKVIASGSKGKEIGNEKWIAIVDLHQISYKNVD--IRGLITGFQFLQNYYPERLG 187
             + L     + S+  +   E    I+DL  IS       +  +       QNYYPER+G
Sbjct: 154 SRYRL----PASSRQADCLVETSCTILDLKGISISAAAQVLSYVREASNIGQNYYPERMG 213

Query: 188 KCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKA 229
           K +++N P+ F + +R+   F+DP T+  I I+ +  + + +++++  E LP ++GG++
Sbjct: 214 KFYMINAPFGFSAAFRLFKPFLDPVTVSKIFILGSSYQ-KELLKQIPAENLPVKFGGQS 266

BLAST of Cp4.1LG01g06130 vs. NCBI nr
Match: XP_023537304.1 (SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484 bits (1247), Expect = 1.43e-172
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. NCBI nr
Match: KAG6599921.1 (hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478 bits (1231), Expect = 3.93e-170
Identity = 240/243 (98.77%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           ME+SRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV
Sbjct: 1   MENSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDP TLKNI IVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. NCBI nr
Match: XP_022941638.1 (SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 476 bits (1226), Expect = 2.27e-169
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFI ESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDP TLKNI IVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. NCBI nr
Match: XP_022996420.1 (SEC14 cytosolic factor-like [Cucurbita maxima])

HSP 1 Score: 469 bits (1206), Expect = 2.55e-166
Identity = 235/243 (96.71%), Postives = 238/243 (97.94%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWR WRDA+V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGS+GKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSEGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDP TLKNI IV N+EERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLP 
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPR 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. NCBI nr
Match: XP_022146735.1 (sec14 cytosolic factor-like [Momordica charantia])

HSP 1 Score: 408 bits (1049), Expect = 2.16e-142
Identity = 207/244 (84.84%), Postives = 222/244 (90.98%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           ME+SRANAL QMRK+VEK G +T+ Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+V
Sbjct: 1   MEESRANALFQMRKSVEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           P+G +CESEV EELEA+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVI
Sbjct: 61  PEGSVCESEVAEELEARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KG+EIGNEKWIAIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-P 240
           VSIWRMVSRFVD  TLKNI IVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ P
Sbjct: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIP 240

Query: 241 HLDA 243
           HLDA
Sbjct: 241 HLDA 243

BLAST of Cp4.1LG01g06130 vs. ExPASy TrEMBL
Match: A0A6J1FSN7 (SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446938 PE=4 SV=1)

HSP 1 Score: 476 bits (1226), Expect = 1.10e-169
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFI ESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDP TLKNI IVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. ExPASy TrEMBL
Match: A0A6J1K6Q5 (SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=1)

HSP 1 Score: 469 bits (1206), Expect = 1.23e-166
Identity = 235/243 (96.71%), Postives = 238/243 (97.94%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWR WRDA+V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGS+GKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSEGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVDP TLKNI IV N+EERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLP 
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPR 240

Query: 241 LDA 243
           LDA
Sbjct: 241 LDA 243

BLAST of Cp4.1LG01g06130 vs. ExPASy TrEMBL
Match: A0A6J1CY34 (sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 SV=1)

HSP 1 Score: 408 bits (1049), Expect = 1.04e-142
Identity = 207/244 (84.84%), Postives = 222/244 (90.98%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           ME+SRANAL QMRK+VEK G +T+ Y DATMMRFLIARSMEP+KGAKMFVQWRKWR+A+V
Sbjct: 1   MEESRANALFQMRKSVEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           P+G +CESEV EELEA+KIFLQG SK GLAV+IV A RHF SKDQLQFKKF+VHLLDKVI
Sbjct: 61  PEGSVCESEVAEELEARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KG+EIGNEKWIAIVDLHQISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVL-P 240
           VSIWRMVSRFVD  TLKNI IVSNEEERR MIEEVGE VLP EYGGKAK IALQDA++ P
Sbjct: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGE-VLPVEYGGKAKLIALQDAIIIP 240

Query: 241 HLDA 243
           HLDA
Sbjct: 241 HLDA 243

BLAST of Cp4.1LG01g06130 vs. ExPASy TrEMBL
Match: A0A6J1FP08 (sec14 cytosolic factor-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446938 PE=4 SV=1)

HSP 1 Score: 397 bits (1019), Expect = 9.29e-139
Identity = 196/198 (98.99%), Postives = 196/198 (98.99%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKGFI ESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKN 198
           VSIWRMVSRFVDP TLKN
Sbjct: 181 VSIWRMVSRFVDPATLKN 198

BLAST of Cp4.1LG01g06130 vs. ExPASy TrEMBL
Match: A0A1S3BT62 (sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1)

HSP 1 Score: 396 bits (1017), Expect = 7.83e-138
Identity = 196/241 (81.33%), Postives = 215/241 (89.21%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           MEDS ANA+  +R ++EKSG +TQ+Y DATMMRFLIARSME SK AKMFVQW+KWRDA V
Sbjct: 1   MEDSNANAVTLLRNSIEKSGFSTQDYSDATMMRFLIARSMEVSKAAKMFVQWKKWRDATV 60

Query: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120
           PKG+I ESEV++EL+AKKIFLQG+S   L VMIV+A RHF SKDQLQFKKF+VH+LDKVI
Sbjct: 61  PKGYIAESEVEDELKAKKIFLQGMSIKQLPVMIVIANRHFHSKDQLQFKKFVVHILDKVI 120

Query: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIAIVDL QISYKNVD RGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240
           VSIWRMVSRFVD  TLKNI IVSNEEE+R+MIEEVGE+VLP EYGGKAKF ALQD V+PH
Sbjct: 181 VSIWRMVSRFVDTATLKNILIVSNEEEKRNMIEEVGEDVLPIEYGGKAKFFALQDVVVPH 240

BLAST of Cp4.1LG01g06130 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 287.0 bits (733), Expect = 1.5e-77
Identity = 137/240 (57.08%), Postives = 187/240 (77.92%), Query Frame = 0

Query: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60
           ME+S+  AL Q+RK+VEK   +T+ Y   T+MRFL+ARSM+P K AKMFV W+KWR ++V
Sbjct: 1   MEESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMV 60

Query: 61  -PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKV 120
            P GFI ESEV++ELE +K+ LQG +K+G  +++V+  +HF+SKD   FKKF+V+ LDK 
Sbjct: 61  PPTGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKT 120

Query: 121 IASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWF 180
           IASG+ GKE+G EK +A++DL  I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP F
Sbjct: 121 IASGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGF 180

Query: 181 FVSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLP 240
           FV++W+ V RF++  T + I IV++ EE+R   EE+G + LPEEYGG+AK  A+QD +LP
Sbjct: 181 FVTVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 240

BLAST of Cp4.1LG01g06130 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 266.2 bits (679), Expect = 2.7e-71
Identity = 125/215 (58.14%), Postives = 169/215 (78.60%), Query Frame = 0

Query: 26  YCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV-PKGFICESEVKEELEAKKIFLQGL 85
           Y   T+MRFL+ARSM+P K AKMFV W+KWR ++V P GFI ESEV++ELE +K+ LQG 
Sbjct: 13  YDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQGP 72

Query: 86  SKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWIAIVDLHQIS 145
           +K+G  +++V+  +HF+SKD   FKKF+V+ LDK IASG+ GKE+G EK +A++DL  I+
Sbjct: 73  TKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLANIT 132

Query: 146 YKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSN 205
           YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++  T + I IV++
Sbjct: 133 YKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIVTD 192

Query: 206 EEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLP 240
            EE+R   EE+G + LPEEYGG+AK  A+QD +LP
Sbjct: 193 GEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of Cp4.1LG01g06130 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 266.2 bits (679), Expect = 2.7e-71
Identity = 125/215 (58.14%), Postives = 169/215 (78.60%), Query Frame = 0

Query: 26  YCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV-PKGFICESEVKEELEAKKIFLQGL 85
           Y   T+MRFL+ARSM+P K AKMFV W+KWR ++V P GFI ESEV++ELE +K+ LQG 
Sbjct: 13  YDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQGP 72

Query: 86  SKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVIASGSKGKEIGNEKWIAIVDLHQIS 145
           +K+G  +++V+  +HF+SKD   FKKF+V+ LDK IASG+ GKE+G EK +A++DL  I+
Sbjct: 73  TKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLANIT 132

Query: 146 YKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDPTTLKNISIVSN 205
           YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++  T + I IV++
Sbjct: 133 YKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIVTD 192

Query: 206 EEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLP 240
            EE+R   EE+G + LPEEYGG+AK  A+QD +LP
Sbjct: 193 GEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of Cp4.1LG01g06130 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 156.8 bits (395), Expect = 2.3e-38
Identity = 79/225 (35.11%), Postives = 134/225 (59.56%), Query Frame = 0

Query: 12  MRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVVPKGFICESEVK 71
           MR   ++    T+E  D  + RFL AR ++  K + MF+ +  W+ +++PKG I E+E+ 
Sbjct: 34  MRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIA 93

Query: 72  EELEAKKIFLQGLSKTGLAVMIVMAKRHFSSK-DQLQFKKFLVHLLDKVIASGSKGKEIG 131
            +L   K+ +QG  K G  + + +  RH  SK +  +FK+F+V+ L+K+ A   +G+   
Sbjct: 94  NDLSHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKICARMPRGQ--- 153

Query: 132 NEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRF 191
            EK++AI DL    Y N DIRG +     LQ+ YPERLGK +I++ P+ F++ W+++  F
Sbjct: 154 -EKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPF 213

Query: 192 VDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQD 236
           +D  T K I  V N++   +++E++ E  LP+ YGGK   + +Q+
Sbjct: 214 IDANTKKKIVFVENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of Cp4.1LG01g06130 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 81.6 bits (200), Expect = 9.3e-16
Identity = 65/231 (28.14%), Postives = 110/231 (47.62%), Query Frame = 0

Query: 2   EDSRANALIQMRKAVEKSGINTQEYC-DATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 61
           +  + N + +++ A+     ++  +C DA++ RFL AR+ +  K  KM  +  KWR    
Sbjct: 14  DSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKAKKMIQETLKWRSTYK 73

Query: 62  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 121
           P+  I  ++V  E E  K            V+++M     +S  Q    + LV+LL+  I
Sbjct: 74  PQE-IRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGNIRHLVYLLENAI 133

Query: 122 ASGSKGKEIGNEKWIAIVDLHQISYK-NVDIRGLITGFQFLQNYYPERLGKCFILNMPWF 181
            +  KG++     W  ++D    S   N  ++        LQNYYPERLG  F+ N P  
Sbjct: 134 INLPKGQK--QMSW--LIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIAFLYNPPRL 193

Query: 182 FVSIWRMVSRFVDPTTLKNISIVSNEEERRS--MIEEVGEEVLPEEYGGKA 229
           F +++R    F+DP T + +  V  +++     M      E LP+E+GG+A
Sbjct: 194 FQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEA 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P242802.5e-1325.51SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ... [more]
P333249.4e-1326.36CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ... [more]
P539891.2e-1224.18SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 376... [more]
Q101371.2e-1224.30Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX... [more]
Q75DK12.1e-1223.85SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC... [more]
Match NameE-valueIdentityDescription
XP_023537304.11.43e-172100.00SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo][more]
KAG6599921.13.93e-17098.77hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941638.12.27e-16998.77SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata][more]
XP_022996420.12.55e-16696.71SEC14 cytosolic factor-like [Cucurbita maxima][more]
XP_022146735.12.16e-14284.84sec14 cytosolic factor-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1FSN71.10e-16998.77SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1K6Q51.23e-16696.71SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=... [more]
A0A6J1CY341.04e-14284.84sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 ... [more]
A0A6J1FP089.29e-13998.99sec14 cytosolic factor-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A1S3BT627.83e-13881.33sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G14820.31.5e-7757.08Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.22.7e-7158.14Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.12.7e-7158.14Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G01630.12.3e-3835.11Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.19.3e-1628.14Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 73..229
e-value: 8.6E-35
score: 131.6
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 72..227
e-value: 9.9E-29
score: 100.1
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 67..232
score: 18.768978
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 71..227
e-value: 5.81045E-29
score: 105.16
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 1..237
e-value: 3.2E-45
score: 156.5
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 68..231
NoneNo IPR availablePANTHERPTHR46277:SF7SEC14 CYTOSOLIC FACTOR-LIKEcoord: 1..239
NoneNo IPR availablePANTHERPTHR46277OS03G0850700 PROTEINcoord: 1..239
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 3..63

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g06130.1Cp4.1LG01g06130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane