Cp4.1LG00g01010 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG00g01010
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAquaporin NIP3-1
LocationCp4.1LG00: 2147425 .. 2149624 (-)
RNA-Seq ExpressionCp4.1LG00g01010
SyntenyCp4.1LG00g01010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCAAATCGAGTGCTTACCCATCGATGCCCTAAAGAGGAGCCAACCTCGCCTTCCGACTCATGTCTCCCTCACTCGCAAGGTACTTATTTTTTGTTTTTTCATGTTCAATCATGTCGAGGATAAGCATTTTAACTATNCTCTCTTCTATTCTTAAATAGAGGGGGGAATTATCATTATTGAGTTGTGAATGATCAAAAGAGTATTTTGCAATATAAAGGTACTCTTTAATCTTCTTCAATTCGAGTCCTTGTTCTTTATTGCCTGAGCTTGACAATTTCATAGGGTATGAAGGTTTGATCATCGTTTTGCTTTTAGTTTTATACTCTTTAATATTAAGTTTACGATCACTATTTCTACCTATAATTTTTTTTTTTGAAGTTTTGCGTAGAATTCAAATGTATTCGAGAAAATAGTATGGAAACCCGAATAATTTTTTGTTAAAAAAATGGGTTCTTTTTCAGTAAAACATAGAGAACGAAGCCATCATCCACTAGAAGCCAATGTCGCAAATCGAGTGCTTACCCATCGATGCCCTAAAGAGGAGCCAACCTCGTCTTCCGACTCATGTCTCCCTCACTCGAAAGGTACTTATTTTTTAATTTTTCATGTTCAATCATGTCGAGGATAAGCATTTTAACTATGATTGAATAAGGAGCTTATTTTATGAAATTGGTGCAAATCACGAACCTACTATTCTAAGTCATATAGAATGGCACATTAGGTTGGTTCGACAGGTTTTTAAGATCAAGTAGTACTATGCTCTTATTTCAACTACAACAATTTATATCATCTAGTCCGTGTGTGAGATCCCACATCGGTCAAAGAGGGAAACAAAGCATTTCTTGTAAAAATGTAATACCGTGAGTCTAACGATGATACGTAACGAGCTAAAGCGGACAATATCTGCTAGCGGTGGGCTTGAATTGTTACAAATGACAATAGATCCAGACATCAAGCTCTGTGCCAGCAAGAGCGCTGGCTCCCAAGGGGGATGAATTGTGAGATCTCACATCAATTAAAGGAGGAAACACAATATTTCTTATAAGGGTGTGATACCATGAGGCTGATGACAATACATAATGGGTTAAAGCAGACAATATCTGCTAGCGGTGGGCTTAGGTTGTTACAAATAATATTAGAGTCAGACATCGGGGGTTTGCCAACGAGGGCGTTGGCTCGGGGGTGGATTGTGATATCCCATATCAGTTGAAGAGTGGAACAAAACATTCCTGATAAGGGGTGGAAACCTCTTCCTAGTAGACACGTTTTAAAATCGTGAGGCTGACAGTTATACGTAACGAGCTAAAGTGGACAATATTTGCTTGGGTTGTTACACCCGACCCGACTCAACCGAATTTTTTTAGGGTTGTAATAGGTTGAAGCTGAATATTTGAGTGATATCATGTAGGTGGGAGCAGAATTTGTGGGCACATTCATCCTCATATTTGCTGCAACAGCCGCACCCATCATCAACCAAAAGTACAACAACATCACCTCTCTAATCGGAAACGCAGCATCCTCCGCCCTAGCGGTGATGATCGTAATTCTGTCGACGGGACACATCTCCGGCGCCCACCTGAACCCCTCACTCACCATCGCCTTAGCCGCCATCCGCCACTTCCCTTGGGTTCAAGTCCCTGCCTACCTCACCGCCCAAATCTCCGCCTCCATATGCGCCTCTTTCACTCTCAAAGGTATCTTTCACCCTTTCATGTCCGGTGGCGTTACAGTTCCCTCCGTCGGCACCGGCCAAGCCTTTGCTCTTGAATTCCTCATTGCCTTCAATCTCTTGTTCGTGGTCACTGCTGTCACTACTGATCCTCGCGTTGTAAGAACAAAAAATAAATCAATAAATCCCTTTAATTGTAAGTTTTTGCTTAATTTATTACTCAAAAATTTAATTATCACAGGTTAGAGAGTTGGCCGGAATTGCCGTCGGAGCTACCGTGATGCTCAACATTCTTATCGCAGGGTCGTCCACCGGTGGTTCAATGAACCCAGTGCGGACTCTTGGCCCCGCCGTGGCCGCCGGAAATTACAGAGGGCTGTGGGTGTACATGGTGGCTCCTATACTTGGGGGGGTTGTCGGTGCCGGAACCTACTCTGCCATGAGAATCAAAGATGATGAAATGGATGTTCCACCGCACGTGACGAGCTTCGGTCAGTACACGAGTGGCAGCTGTGTTCGTGTTTAA

mRNA sequence

ATGTCGCAAATCGAGTGCTTACCCATCGATGCCCTAAAGAGGAGCCAACCTCGCCTTCCGACTCATGTCTCCCTCACTCGCAAGGTGGGAGCAGAATTTGTGGGCACATTCATCCTCATATTTGCTGCAACAGCCGCACCCATCATCAACCAAAAGTACAACAACATCACCTCTCTAATCGGAAACGCAGCATCCTCCGCCCTAGCGGTGATGATCGTAATTCTGTCGACGGGACACATCTCCGGCGCCCACCTGAACCCCTCACTCACCATCGCCTTAGCCGCCATCCGCCACTTCCCTTGGGTTCAAGTCCCTGCCTACCTCACCGCCCAAATCTCCGCCTCCATATGCGCCTCTTTCACTCTCAAAGGTATCTTTCACCCTTTCATGTCCGGTGGCGTTACAGTTCCCTCCGTCGGCACCGGCCAAGCCTTTGCTCTTGAATTCCTCATTGCCTTCAATCTCTTGTTCGTGGTCACTGCTGTCACTACTGATCCTCGCGTTGTTAGAGAGTTGGCCGGAATTGCCGTCGGAGCTACCGTGATGCTCAACATTCTTATCGCAGGGTCGTCCACCGGTGGTTCAATGAACCCAGTGCGGACTCTTGGCCCCGCCGTGGCCGCCGGAAATTACAGAGGGCTGTGGGTGTACATGGTGGCTCCTATACTTGGGGGGGTTGTCGGTGCCGGAACCTACTCTGCCATGAGAATCAAAGATGATGAAATGGATGTTCCACCGCACGTGACGAGCTTCGGTCAGTACACGAGTGGCAGCTGTGTTCGTGTTTAA

Coding sequence (CDS)

ATGTCGCAAATCGAGTGCTTACCCATCGATGCCCTAAAGAGGAGCCAACCTCGCCTTCCGACTCATGTCTCCCTCACTCGCAAGGTGGGAGCAGAATTTGTGGGCACATTCATCCTCATATTTGCTGCAACAGCCGCACCCATCATCAACCAAAAGTACAACAACATCACCTCTCTAATCGGAAACGCAGCATCCTCCGCCCTAGCGGTGATGATCGTAATTCTGTCGACGGGACACATCTCCGGCGCCCACCTGAACCCCTCACTCACCATCGCCTTAGCCGCCATCCGCCACTTCCCTTGGGTTCAAGTCCCTGCCTACCTCACCGCCCAAATCTCCGCCTCCATATGCGCCTCTTTCACTCTCAAAGGTATCTTTCACCCTTTCATGTCCGGTGGCGTTACAGTTCCCTCCGTCGGCACCGGCCAAGCCTTTGCTCTTGAATTCCTCATTGCCTTCAATCTCTTGTTCGTGGTCACTGCTGTCACTACTGATCCTCGCGTTGTTAGAGAGTTGGCCGGAATTGCCGTCGGAGCTACCGTGATGCTCAACATTCTTATCGCAGGGTCGTCCACCGGTGGTTCAATGAACCCAGTGCGGACTCTTGGCCCCGCCGTGGCCGCCGGAAATTACAGAGGGCTGTGGGTGTACATGGTGGCTCCTATACTTGGGGGGGTTGTCGGTGCCGGAACCTACTCTGCCATGAGAATCAAAGATGATGAAATGGATGTTCCACCGCACGTGACGAGCTTCGGTCAGTACACGAGTGGCAGCTGTGTTCGTGTTTAA

Protein sequence

MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSFGQYTSGSCVRV
Homology
BLAST of Cp4.1LG00g01010 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.9e-92
Identity = 173/229 (75.55%), Postives = 193/229 (84.28%), Query Frame = 0

Query: 23  VSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGHISG 82
           VSLTRK+GAEFVGTFILIF ATA PI+NQKY+   +LIGNAA + LAVMI+ILSTGHISG
Sbjct: 74  VSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISG 133

Query: 83  AHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTG 142
           AHLNPSLTIA AA+RHFPW  VPAY+ AQ+SASICASF LKG+FHPFMSGGVT+PSV  G
Sbjct: 134 AHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLG 193

Query: 143 QAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPVRTL 202
           QAFALEF+I F LLFVVTAV TD R V ELAGIAVGATVMLNIL+AG STGGSMNPVRTL
Sbjct: 194 QAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTL 253

Query: 203 GPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSF 252
           GPAVA+GNYR LWVY+VAP LG + GA  Y+ +++ D   D P  V SF
Sbjct: 254 GPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDPPRPVRSF 302

BLAST of Cp4.1LG00g01010 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 318.9 bits (816), Expect = 5.2e-86
Identity = 169/261 (64.75%), Postives = 197/261 (75.48%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQP-------RLPT-HVSLTRKVGAEFVGTFILIFAATAAPIINQK 60
           M + +CLP    +   P        +P   VSLTRK+GAEFVGTFILIF ATAAPI+NQK
Sbjct: 49  MPRCKCLPAAVAEAWAPSAHGCVVEIPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQK 108

Query: 61  YNNITSLIGNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQI 120
           Y    S  GNAA + LAV  +ILSTGHISGAHLNPSLTIA AA+RHFPW+QVPAY+  Q+
Sbjct: 109 YGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQV 168

Query: 121 SASICASFTLKGIFHPFMSGGVTV--PSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVR 180
             SICA F LKG+FHPF+SGGVTV  P++ T QAF  EF+I FNLLFVVTAV TD R V 
Sbjct: 169 LGSICAGFALKGVFHPFLSGGVTVPDPTISTAQAFFTEFIITFNLLFVVTAVATDTRAVG 228

Query: 181 ELAGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAG 240
           ELAGIAVGA V LNILIAG +TGGSMNPVRTLGPAVAAGNYR LW+Y++AP LG V GAG
Sbjct: 229 ELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLIAPTLGAVAGAG 288

Query: 241 TYSAMRIKDDEMDVPPHVTSF 252
            Y+A++++D+  + P    SF
Sbjct: 289 VYTAVKLRDENGETPRPQRSF 309

BLAST of Cp4.1LG00g01010 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 4.4e-85
Identity = 168/261 (64.37%), Postives = 199/261 (76.25%), Query Frame = 0

Query: 1   MSQIECLPIDALK-------RSQPRLPT-HVSLTRKVGAEFVGTFILIFAATAAPIINQK 60
           M + +CLP+ A++            +P   VSLTRK+GAEFVGTFILIF ATAAPI+NQK
Sbjct: 40  MPRCKCLPLPAVEGWGVATHTCVVEIPAPDVSLTRKLGAEFVGTFILIFFATAAPIVNQK 99

Query: 61  YNNITSLIGNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQI 120
           Y    S  GNAA + LAV  VILSTGHISGAHLNPSLTIA AA+RHFPW+QVPAY+  Q 
Sbjct: 100 YGGAISPFGNAACAGLAVATVILSTGHISGAHLNPSLTIAFAALRHFPWLQVPAYVAVQA 159

Query: 121 SASICASFTLKGIFHPFMSGGVTVP--SVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVR 180
            AS+CA+F LKG+FHPF+SGGVTVP  +V T QAF  EF+I+FNLLFVVTAV TD R V 
Sbjct: 160 LASVCAAFALKGVFHPFLSGGVTVPDATVSTAQAFFTEFIISFNLLFVVTAVATDTRAVG 219

Query: 181 ELAGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAG 240
           ELAGIAVGA V LNIL+AG +TGGSMNPVRTLGPAVAAGNYR LW+Y++AP LG + GA 
Sbjct: 220 ELAGIAVGAAVTLNILVAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLLAPTLGALAGAS 279

Query: 241 TYSAMRIKDDEMDVPPHVTSF 252
            Y A++++D+  + P    SF
Sbjct: 280 VYKAVKLRDENGETPRTQRSF 300

BLAST of Cp4.1LG00g01010 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-80
Identity = 155/232 (66.81%), Postives = 188/232 (81.03%), Query Frame = 0

Query: 20  PTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGH 79
           P +VSL RK+GAEFVGT ILIFA TA  I+NQK +   +LIG AAS+ LAVMIVILSTGH
Sbjct: 73  PPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVILSTGH 132

Query: 80  ISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSV 139
           ISGAHLNP++TIA AA++HFPW  VP Y+ AQ+ AS+ A+F LK +F P MSGGVTVP+V
Sbjct: 133 ISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPTV 192

Query: 140 GTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPV 199
           G  QAFALEF+I+FNL+FVVTAV TD R V ELAGIAVGATVMLNILIAG +T  SMNPV
Sbjct: 193 GLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPATSASMNPV 252

Query: 200 RTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSF 252
           RTLGPA+AA NYR +WVY+ APILG ++GAGTY+ +++ +++ + P    SF
Sbjct: 253 RTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED-EAPKERRSF 303

BLAST of Cp4.1LG00g01010 vs. ExPASy Swiss-Prot
Match: Q84S07 (Aquaporin NIP3-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-3 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-57
Identity = 111/219 (50.68%), Postives = 159/219 (72.60%), Query Frame = 0

Query: 19  LPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTG 78
           +P+ V L +KV AEF GTFILIF   +  I+++++ +I +L+G A S+ LAV +++LS  
Sbjct: 60  IPSSVPLLKKVSAEFFGTFILIFTVLSTIIMDEQHKSIETLLGIATSAGLAVTVLVLSLI 119

Query: 79  HISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPS 138
           HISG HLNP+++IA+A   H P   +  Y+++QI  ++ ASF +KG++HP   G VTVP+
Sbjct: 120 HISGCHLNPAISIAMAVFGHLPSAHLLPYISSQILGAVAASFAVKGLYHPVNPGIVTVPN 179

Query: 139 VGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNP 198
           VGT +AF +EF+I F LLF++TA+ TDP  V+EL  +AVGATVM+NIL+AG STG SMNP
Sbjct: 180 VGTVEAFFVEFIITFFLLFIITALATDPNAVKELIAVAVGATVMMNILVAGPSTGASMNP 239

Query: 199 VRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRI 238
            RT+G A+A G Y  +WVY+VA  LG + G G Y A+++
Sbjct: 240 ARTIGAAIATGRYTQIWVYLVATPLGAIAGTGAYVAIKL 278

BLAST of Cp4.1LG00g01010 vs. NCBI nr
Match: XP_023520518.1 (probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520519.1 probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520520.1 probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520521.1 probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520522.1 probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520523.1 probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520524.1 probable aquaporin NIP5-1 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023520525.1 probable aquaporin NIP5-1 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023520526.1 probable aquaporin NIP5-1 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023520527.1 probable aquaporin NIP5-1 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023520528.1 probable aquaporin NIP5-1 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508 bits (1308), Expect = 3.11e-181
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMDVPPHVTSFGQYTSGSCVRV
Sbjct: 241 EMDVPPHVTSFGQYTSGSCVRV 262

BLAST of Cp4.1LG00g01010 vs. NCBI nr
Match: XP_022928964.1 (probable aquaporin NIP5-1 [Cucurbita moschata] >XP_022928965.1 probable aquaporin NIP5-1 [Cucurbita moschata] >XP_022928966.1 probable aquaporin NIP5-1 [Cucurbita moschata])

HSP 1 Score: 500 bits (1288), Expect = 3.48e-178
Identity = 259/262 (98.85%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSV TGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVSTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMDVP HV SFGQYTSGSCVRV
Sbjct: 241 EMDVPQHVRSFGQYTSGSCVRV 262

BLAST of Cp4.1LG00g01010 vs. NCBI nr
Match: KAG6579619.1 (putative aquaporin NIP5-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 497 bits (1280), Expect = 5.76e-177
Identity = 255/262 (97.33%), Postives = 258/262 (98.47%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASS LAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSGLAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRE+AGIAVGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVREMAGIAVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGG+VGAGTYSAMRIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGIVGAGTYSAMRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMDVP HV SFGQYT G+CVRV
Sbjct: 241 EMDVPQHVRSFGQYTGGNCVRV 262

BLAST of Cp4.1LG00g01010 vs. NCBI nr
Match: XP_022969785.1 (probable aquaporin NIP5-1 [Cucurbita maxima] >XP_022969786.1 probable aquaporin NIP5-1 [Cucurbita maxima] >XP_022969787.1 probable aquaporin NIP5-1 [Cucurbita maxima] >XP_022969788.1 probable aquaporin NIP5-1 [Cucurbita maxima])

HSP 1 Score: 485 bits (1249), Expect = 3.06e-172
Identity = 249/262 (95.04%), Postives = 254/262 (96.95%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDA KRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDAPKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASS LAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSGLAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSVGTGQAFALEFLI FNLLFVVTAVTTD RVVRELAGI+VGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVTTDTRVVRELAGISVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNY+ LWVYMVAPILGG+VGAG YSA+RIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYQELWVYMVAPILGGIVGAGIYSALRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMD+PPHV SFGQYT GSCVRV
Sbjct: 241 EMDIPPHVRSFGQYTGGSCVRV 262

BLAST of Cp4.1LG00g01010 vs. NCBI nr
Match: KAG7017077.1 (putative aquaporin NIP5-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441 bits (1135), Expect = 2.62e-155
Identity = 227/234 (97.01%), Postives = 230/234 (98.29%), Query Frame = 0

Query: 29  VGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGHISGAHLNPS 88
           VGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASS LAVMIVILSTGHISGAHLNPS
Sbjct: 1   VGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSGLAVMIVILSTGHISGAHLNPS 60

Query: 89  LTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTGQAFALE 148
           LTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTGQAFALE
Sbjct: 61  LTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTGQAFALE 120

Query: 149 FLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAA 208
           FLIAFNLLFVVTAVTTDPRVVRE+AGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAA
Sbjct: 121 FLIAFNLLFVVTAVTTDPRVVREMAGIAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAA 180

Query: 209 GNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSFGQYTSGSCVRV 262
           GNYRGLWVYMVAPILGG+VGAGTYSAMRIKDDEMDVP HV SFGQYT G+CVRV
Sbjct: 181 GNYRGLWVYMVAPILGGIVGAGTYSAMRIKDDEMDVPQHVRSFGQYTGGNCVRV 234

BLAST of Cp4.1LG00g01010 vs. ExPASy TrEMBL
Match: A0A6J1ELS3 (probable aquaporin NIP5-1 OS=Cucurbita moschata OX=3662 GN=LOC111435708 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 1.68e-178
Identity = 259/262 (98.85%), Postives = 259/262 (98.85%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSV TGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVSTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMDVP HV SFGQYTSGSCVRV
Sbjct: 241 EMDVPQHVRSFGQYTSGSCVRV 262

BLAST of Cp4.1LG00g01010 vs. ExPASy TrEMBL
Match: A0A6J1HXA4 (probable aquaporin NIP5-1 OS=Cucurbita maxima OX=3661 GN=LOC111468885 PE=3 SV=1)

HSP 1 Score: 485 bits (1249), Expect = 1.48e-172
Identity = 249/262 (95.04%), Postives = 254/262 (96.95%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60
           MSQIECLPIDA KRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI
Sbjct: 1   MSQIECLPIDAPKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLI 60

Query: 61  GNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120
           GNAASS LAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF
Sbjct: 61  GNAASSGLAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASF 120

Query: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGAT 180
           TLKGIFHPFMSGGVTVPSVGTGQAFALEFLI FNLLFVVTAVTTD RVVRELAGI+VGAT
Sbjct: 121 TLKGIFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVTTDTRVVRELAGISVGAT 180

Query: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDD 240
           VMLNILIAGSSTGGSMNPVRTLGPAVAAGNY+ LWVYMVAPILGG+VGAG YSA+RIKDD
Sbjct: 181 VMLNILIAGSSTGGSMNPVRTLGPAVAAGNYQELWVYMVAPILGGIVGAGIYSALRIKDD 240

Query: 241 EMDVPPHVTSFGQYTSGSCVRV 262
           EMD+PPHV SFGQYT GSCVRV
Sbjct: 241 EMDIPPHVRSFGQYTGGSCVRV 262

BLAST of Cp4.1LG00g01010 vs. ExPASy TrEMBL
Match: A0A0A0KNJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146200 PE=3 SV=1)

HSP 1 Score: 380 bits (976), Expect = 3.59e-131
Identity = 198/247 (80.16%), Postives = 214/247 (86.64%), Query Frame = 0

Query: 5   ECLPIDALKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAA 64
           EC+ +D+ KRS       VSLTRKV AEFVGTFILIF ATAAPIINQKYN+  SLIGNAA
Sbjct: 4   ECIHVDSPKRSHT---LDVSLTRKVAAEFVGTFILIFGATAAPIINQKYNSPMSLIGNAA 63

Query: 65  SSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKG 124
            + LAVMIVILS GHISGAHLNPSLTIALA +RHF W  VPAY+TAQ+SASICASFTLKG
Sbjct: 64  CAGLAVMIVILSIGHISGAHLNPSLTIALATLRHFAWAHVPAYITAQVSASICASFTLKG 123

Query: 125 IFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLN 184
           +FHPFMSGGVTVPSVGTGQAFALEFLI FNLLFVVTAV TD R VRELAGI VGATVMLN
Sbjct: 124 VFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVRELAGIGVGATVMLN 183

Query: 185 ILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDV 244
           ILIAG STGGSMNPVRTLGPAVAAGNYR LW+YMVAP LG +VGAGTY+A++ KDD +DV
Sbjct: 184 ILIAGPSTGGSMNPVRTLGPAVAAGNYRELWIYMVAPTLGAIVGAGTYTAVKHKDDGIDV 243

Query: 245 PPHVTSF 251
            P V+SF
Sbjct: 244 LPEVSSF 247

BLAST of Cp4.1LG00g01010 vs. ExPASy TrEMBL
Match: A0A1S3AU64 (probable aquaporin NIP5-1 OS=Cucumis melo OX=3656 GN=LOC103482897 PE=3 SV=1)

HSP 1 Score: 380 bits (976), Expect = 2.02e-130
Identity = 192/257 (74.71%), Postives = 218/257 (84.82%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRL------PTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYN 60
           M + +CLP++A    QP           VSLTRK+GAEFVGTFILIFAATA PI+NQKYN
Sbjct: 40  MPRCKCLPVNAPTWGQPHTCFTDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYN 99

Query: 61  NITSLIGNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISA 120
            + +LIGNAA + LAVMIVILSTGHISGAHLNPSLTIA AA+RHFPWVQVPAY+ AQ+SA
Sbjct: 100 GVETLIGNAACAGLAVMIVILSTGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSA 159

Query: 121 SICASFTLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAG 180
           SICASF LKG+FHPFMSGGVTVPSV TGQAFALEF+I FNLLFVVTAV TD R V ELAG
Sbjct: 160 SICASFALKGVFHPFMSGGVTVPSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAG 219

Query: 181 IAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSA 240
           IAVGATVMLNIL+AG S+GGSMNPVRTLGPAVAAGNYR LWVY+VAP LG ++GAGTY+A
Sbjct: 220 IAVGATVMLNILVAGPSSGGSMNPVRTLGPAVAAGNYRALWVYLVAPTLGAIIGAGTYTA 279

Query: 241 MRIKDDEMDVPPHVTSF 251
           ++++DDE++ P  V SF
Sbjct: 280 VKLRDDEVEAPSQVRSF 296

BLAST of Cp4.1LG00g01010 vs. ExPASy TrEMBL
Match: A0A0A0KQH1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146190 PE=3 SV=1)

HSP 1 Score: 378 bits (971), Expect = 1.16e-129
Identity = 191/257 (74.32%), Postives = 217/257 (84.44%), Query Frame = 0

Query: 1   MSQIECLPIDALKRSQPRL------PTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYN 60
           M + +CLP++A    QP           VSLTRK+GAEFVGTFILIFAATA PI+NQKYN
Sbjct: 40  MPRCKCLPVNAPTWGQPHTCFTDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYN 99

Query: 61  NITSLIGNAASSALAVMIVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISA 120
            + +LIGNAA + LAVMIVILSTGHISGAHLNPSLTIA AA+RHFPWVQVPAY+ AQ+SA
Sbjct: 100 GVETLIGNAACAGLAVMIVILSTGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSA 159

Query: 121 SICASFTLKGIFHPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAG 180
           SICASF LKG+FHPFMSGGVTVPSV  GQAFALEF+I FNLLFVVTAV TD R V ELAG
Sbjct: 160 SICASFALKGVFHPFMSGGVTVPSVSIGQAFALEFIITFNLLFVVTAVATDTRAVGELAG 219

Query: 181 IAVGATVMLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSA 240
           IAVGATVMLNIL+AG S+GGSMNPVRTLGPAVAAGNY+ LWVY+VAP LG ++GAGTY+A
Sbjct: 220 IAVGATVMLNILVAGPSSGGSMNPVRTLGPAVAAGNYKALWVYLVAPTLGAIIGAGTYTA 279

Query: 241 MRIKDDEMDVPPHVTSF 251
           ++++DDE+D P  V SF
Sbjct: 280 VKLRDDEVDAPSQVRSF 296

BLAST of Cp4.1LG00g01010 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 339.7 bits (870), Expect = 2.0e-93
Identity = 173/229 (75.55%), Postives = 193/229 (84.28%), Query Frame = 0

Query: 23  VSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGHISG 82
           VSLTRK+GAEFVGTFILIF ATA PI+NQKY+   +LIGNAA + LAVMI+ILSTGHISG
Sbjct: 74  VSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISG 133

Query: 83  AHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSVGTG 142
           AHLNPSLTIA AA+RHFPW  VPAY+ AQ+SASICASF LKG+FHPFMSGGVT+PSV  G
Sbjct: 134 AHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLG 193

Query: 143 QAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPVRTL 202
           QAFALEF+I F LLFVVTAV TD R V ELAGIAVGATVMLNIL+AG STGGSMNPVRTL
Sbjct: 194 QAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTL 253

Query: 203 GPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSF 252
           GPAVA+GNYR LWVY+VAP LG + GA  Y+ +++ D   D P  V SF
Sbjct: 254 GPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDPPRPVRSF 302

BLAST of Cp4.1LG00g01010 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 301.2 bits (770), Expect = 8.0e-82
Identity = 155/232 (66.81%), Postives = 188/232 (81.03%), Query Frame = 0

Query: 20  PTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGH 79
           P +VSL RK+GAEFVGT ILIFA TA  I+NQK +   +LIG AAS+ LAVMIVILSTGH
Sbjct: 73  PPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVILSTGH 132

Query: 80  ISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFHPFMSGGVTVPSV 139
           ISGAHLNP++TIA AA++HFPW  VP Y+ AQ+ AS+ A+F LK +F P MSGGVTVP+V
Sbjct: 133 ISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPTV 192

Query: 140 GTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMNPV 199
           G  QAFALEF+I+FNL+FVVTAV TD R V ELAGIAVGATVMLNILIAG +T  SMNPV
Sbjct: 193 GLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPATSASMNPV 252

Query: 200 RTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKDDEMDVPPHVTSF 252
           RTLGPA+AA NYR +WVY+ APILG ++GAGTY+ +++ +++ + P    SF
Sbjct: 253 RTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED-EAPKERRSF 303

BLAST of Cp4.1LG00g01010 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-49
Identity = 101/222 (45.50%), Postives = 143/222 (64.41%), Query Frame = 0

Query: 20  PTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVMIVILSTGH 79
           P+ V LT+K+ AE +GT+ +IF+     ++N  Y    +  G   +  L VM++I STGH
Sbjct: 36  PSIVCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGH 95

Query: 80  ISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIFH--PFMSGGVTVP 139
           ISGAH NP++T+  A  R FPW QVP Y+ AQ++ S+ AS TL+ +F+  P    G T P
Sbjct: 96  ISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFG-TTP 155

Query: 140 SVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVMLNILIAGSSTGGSMN 199
           +  +GQA   E +I+F L+FV++ V TD R   ELAGIAVG T++LN+ +AG  +G SMN
Sbjct: 156 TDSSGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMN 215

Query: 200 PVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKD 240
           P R+LGPA+  G Y+G+WVY+V P +G   G   Y+ MR  D
Sbjct: 216 PARSLGPAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTD 256

BLAST of Cp4.1LG00g01010 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 190.7 bits (483), Expect = 1.5e-48
Identity = 103/236 (43.64%), Postives = 153/236 (64.83%), Query Frame = 0

Query: 12  LKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVM 71
           LK+    L   V   +K+ AEF+GT+ L+F   A+ ++N + +N+ +L G A    L +M
Sbjct: 40  LKKQDSLLSVSVPFLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIM 99

Query: 72  IVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLK---GIFHP 131
           ++I S GHISGAH+NP++TIA A+   FP  QVPAY+ +Q+  S  A+ TL+   G+ H 
Sbjct: 100 VLIYSLGHISGAHINPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHD 159

Query: 132 FMSG--GVTVPSVGTG---QAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATVML 191
             SG   V + S   G   QAF +EF++ F L+F+++ V TD R + ELAG+A+G+TV+L
Sbjct: 160 VCSGKHDVFIGSSPVGSDLQAFTMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLL 219

Query: 192 NILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKD 240
           N+LIA   +  SMNP R+LGPA+  G Y+G+W+Y+VAP LG + GA  Y+ +R  D
Sbjct: 220 NVLIAAPVSSASMNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTD 275

BLAST of Cp4.1LG00g01010 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 189.1 bits (479), Expect = 4.5e-48
Identity = 107/238 (44.96%), Postives = 151/238 (63.45%), Query Frame = 0

Query: 12  LKRSQPRLPTHVSLTRKVGAEFVGTFILIFAATAAPIINQKYNNITSLIGNAASSALAVM 71
           LK+    L   V   +K+ AE +GT+ LIFA  AA  +N +++   +L G A    L VM
Sbjct: 37  LKKQDSLLSISVPFLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVM 96

Query: 72  IVILSTGHISGAHLNPSLTIALAAIRHFPWVQVPAYLTAQISASICASFTLKGIF----- 131
           +++ S GHISGAH NP++TIA A+   FP  QVPAY+ +Q+  S  A+ TL+ +F     
Sbjct: 97  VLVYSLGHISGAHFNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQD 156

Query: 132 -----HPFMSGGVTVPSVGTGQAFALEFLIAFNLLFVVTAVTTDPRVVRELAGIAVGATV 191
                H    G  T+PS    Q+F +EF+I F L+FV++ V TD R + ELAG+AVG+TV
Sbjct: 157 VCSGKHDVFVG--TLPSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTV 216

Query: 192 MLNILIAGSSTGGSMNPVRTLGPAVAAGNYRGLWVYMVAPILGGVVGAGTYSAMRIKD 240
           +LN++IAG  +G SMNP R+LGPA+    YRGLW+Y+V+PI+G V GA  Y+ +R  D
Sbjct: 217 LLNVIIAGPVSGASMNPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTD 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SV842.9e-9275.55Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1[more]
Q0IWF35.2e-8664.75Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2[more]
Q9ATN14.4e-8564.37Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1[more]
Q9SAI41.1e-8066.81Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1[more]
Q84S071.3e-5750.68Aquaporin NIP3-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023520518.13.11e-181100.00probable aquaporin NIP5-1 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520519.... [more]
XP_022928964.13.48e-17898.85probable aquaporin NIP5-1 [Cucurbita moschata] >XP_022928965.1 probable aquapori... [more]
KAG6579619.15.76e-17797.33putative aquaporin NIP5-1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022969785.13.06e-17295.04probable aquaporin NIP5-1 [Cucurbita maxima] >XP_022969786.1 probable aquaporin ... [more]
KAG7017077.12.62e-15597.01putative aquaporin NIP5-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1ELS31.68e-17898.85probable aquaporin NIP5-1 OS=Cucurbita moschata OX=3662 GN=LOC111435708 PE=3 SV=... [more]
A0A6J1HXA41.48e-17295.04probable aquaporin NIP5-1 OS=Cucurbita maxima OX=3661 GN=LOC111468885 PE=3 SV=1[more]
A0A0A0KNJ13.59e-13180.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146200 PE=3 SV=1[more]
A0A1S3AU642.02e-13074.71probable aquaporin NIP5-1 OS=Cucumis melo OX=3656 GN=LOC103482897 PE=3 SV=1[more]
A0A0A0KQH11.16e-12974.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146190 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10380.12.0e-9375.55NOD26-like intrinsic protein 5;1 [more]
AT1G80760.18.0e-8266.81NOD26-like intrinsic protein 6;1 [more]
AT5G37820.11.4e-4945.50NOD26-like intrinsic protein 4;2 [more]
AT4G19030.11.5e-4843.64NOD26-like major intrinsic protein 1 [more]
AT4G18910.14.5e-4844.96NOD26-like intrinsic protein 1;2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 27..46
score: 34.55
coord: 215..235
score: 33.76
coord: 147..165
score: 27.62
coord: 178..200
score: 40.02
coord: 103..122
score: 25.25
coord: 66..90
score: 40.2
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 22..232
e-value: 4.4E-50
score: 170.5
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 10..245
e-value: 1.2E-64
score: 219.6
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 19..242
NoneNo IPR availablePANTHERPTHR45724:SF33AQUAPORIN NIP5-1 ISOFORM X1-RELATEDcoord: 3..248
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 3..248
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 84..92

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG00g01010.1Cp4.1LG00g01010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity