CmoCh19G009950 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh19G009950 vs. ExPASy Swiss-Prot
Match: F4IDS7 (Vacuolar sorting protein 18 OS=Arabidopsis thaliana OX=3702 GN=VPS18 PE=1 SV=1)

HSP 1 Score: 1547.3 bits (4005), Expect = 0.0e+00
Identity = 754/988 (76.32%), Postives = 863/988 (87.35%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MD GR  F+VDLLERYA K RG+I+CM+AGNDVI+LGTSKGW+ RYDFG+G S D DL+V
Sbjct: 1   MDQGRQVFSVDLLERYATKNRGMITCMAAGNDVIVLGTSKGWIIRYDFGVGSSNDIDLAV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GR GEQSIH++FVDPGGSHCI TV G  GA+TFY HAKW KPR+L RLKGL+VN VAWNR
Sbjct: 61  GRTGEQSIHKVFVDPGGSHCIATVTGVGGAETFYTHAKWLKPRVLSRLKGLLVNAVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           Q ITE STKE+ILGT +GQLFE+AVDEK+K+EKY+KFLFEL ELPEAF  LQMET ++ +
Sbjct: 121 QQITEVSTKEIILGTQDGQLFEMAVDEKDKREKYIKFLFELEELPEAFKALQMETANISS 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY-------LPSSELHFYIKQRRAM 240
           GMR+YVMAVTPTRLYSFTGIG+LE  ++ S +  A +       +P+SELHF+IKQRRA+
Sbjct: 181 GMRYYVMAVTPTRLYSFTGIGTLE-SVFASYKERAVHFMELPGEIPNSELHFFIKQRRAV 240

Query: 241 HFAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFH 300
           HFAWLSG GIYHG LNFG+QHS  NGDENFVENKALLDYSKL++ + +VKP S+A+SE+H
Sbjct: 241 HFAWLSGTGIYHGGLNFGAQHSYPNGDENFVENKALLDYSKLSDGTEAVKPGSMALSEYH 300

Query: 301 FLLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVS 360
           FLLLIGNKVKVVNRI+EQI+EELQFD T++++ RGI+GLCSDA+A +FYAYDQNSIFQVS
Sbjct: 301 FLLLIGNKVKVVNRISEQIIEELQFDITSDSVSRGIIGLCSDASANVFYAYDQNSIFQVS 360

Query: 361 VNDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINY 420
           V DEGRDMWKVYLD+K Y A+LA+CRD LQRDQVYL QAE A   ++YLRAASFYAKINY
Sbjct: 361 VIDEGRDMWKVYLDLKVYAAALANCRDPLQRDQVYLVQAESAFTDKEYLRAASFYAKINY 420

Query: 421 ILSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLD 480
           ++SFEE+TLKFIS +E +ALRTFLL KLDNL+KDDKCQITMISTWATELYLDKINRLLL+
Sbjct: 421 VISFEEVTLKFISINEPEALRTFLLHKLDNLSKDDKCQITMISTWATELYLDKINRLLLE 480

Query: 481 DDIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEI 540
           DD A +   ++Y S+I+EFRAF+SD KD LDE TT+K+LESYGRVEELV+FA LKEQYEI
Sbjct: 481 DDTAIENRDSEYHSVIQEFRAFMSDCKDELDEATTVKILESYGRVEELVYFANLKEQYEI 540

Query: 541 VVHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIP 600
           VV HYIQQGEAKKALEVLQK  V  +LQY+FAPELIMLDAYETVESWM   NLNPR+LI 
Sbjct: 541 VVLHYIQQGEAKKALEVLQKSSVSVELQYQFAPELIMLDAYETVESWMANKNLNPRRLIT 600

Query: 601 AMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCK 660
           AMMRYS  PHAKNETHEVIKYLE+CVHRLHNEDPG+H+LLLSLYAKQEDD ALLRFLQCK
Sbjct: 601 AMMRYSSGPHAKNETHEVIKYLEFCVHRLHNEDPGIHSLLLSLYAKQEDDGALLRFLQCK 660

Query: 661 FGKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEA 720
           FGKG ENGP+FFYDPKYALRLCLKE+R RACVHIYSMM+MHEEAVALALQ+D ELAMAEA
Sbjct: 661 FGKGRENGPEFFYDPKYALRLCLKERRTRACVHIYSMMSMHEEAVALALQIDPELAMAEA 720

Query: 721 DKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFA 780
           DKVEDDEDLRKKLWLM+AKHV++ EKG KRENIRKAIAFLKETDGLLKIEDILPFFPDFA
Sbjct: 721 DKVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFA 780

Query: 781 LIDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVC 840
           LIDDFKEAICSSLEDYNKQI+QLK+EMNDAT GADNIR DI+AL QRYA+IDRDE+CGVC
Sbjct: 781 LIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGVC 840

Query: 841 KRKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQ 900
           KRKIL +  D+ MA  Y+S   +APFYVFPCGH FHAQCLI HVT C  E QAE+ILDLQ
Sbjct: 841 KRKILMMSGDFRMAQGYSSAGPLAPFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQ 900

Query: 901 KQITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFI 960
           KQ+TLLG ETRR+ NG+ +++ I+S T ADKLR++LDDAIA ECPFCGELMI EI+LPFI
Sbjct: 901 KQLTLLGSETRRDINGNRSDEPITSTTTADKLRSELDDAIASECPFCGELMINEITLPFI 960

Query: 961 SSEEAQQVSSWEIRPH-NLGGQRSFSLP 981
             E++Q  +SW++R   NL  QR+ SLP
Sbjct: 961 KPEDSQYSTSWDLRSETNLANQRTISLP 987

BLAST of CmoCh19G009950 vs. ExPASy Swiss-Prot
Match: P59015 (Vacuolar protein sorting-associated protein 18 homolog OS=Danio rerio OX=7955 GN=vps18 PE=2 SV=2)

HSP 1 Score: 521.9 bits (1343), Expect = 1.5e-146
Identity = 337/974 (34.60%), Postives = 537/974 (55.13%), Query Frame = 0

Query: 26  CMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSVGRPGEQSIHRIFVDPGGSH---CIT 85
           CMS G D +L         R D G  D  +  + +GR  +  +HR+F+DP GSH   C+T
Sbjct: 68  CMSLGKDTLL---------RIDLGKPDQPN-QIELGRKDDSKVHRLFLDPTGSHLVICLT 127

Query: 86  TVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFE 145
           T       +  Y++    K R L R +G ++ ++ WN+   +E +T  +++GT  G +FE
Sbjct: 128 T------NECVYLNRNTQKVRGLSRWRGHLIESIGWNKLIGSETNTGPILVGTSQGIIFE 187

Query: 146 LAVDEKE------KKEKYVKFLFELAELPEAFMDLQMETTSVLNGMRFYVMAVTPTRLYS 205
             +   E        ++Y + +  L E  +      +E    L   +++++A T  RL+ 
Sbjct: 188 AEISASEGSLFNTNPDQYFRQVHYLEEDGKPAPVCCLEVERGLE-TKYFIIATTRKRLFQ 247

Query: 206 FTG---IGSLEMGLYTSLEHYAPYLPS----------SELHFYIKQRRA--MHFAWLSGA 265
           F G    GS + G  +        LPS          SE+ FY  + R+    FAW+ G 
Sbjct: 248 FVGKLAEGSEQQGFSSIFAQNQDLLPSFQEFPVNMGYSEITFYTSKLRSRPKTFAWMMGN 307

Query: 266 GIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHFLLLIGNK 325
           G+ +G+L++          ++ + +  + +Y++   +   VKP S+ +++FHFLLL+ ++
Sbjct: 308 GVLYGQLDYVR-------PDSLLSDVQVWEYTQ-DIDLNFVKPISIVLTQFHFLLLLPDR 367

Query: 326 VKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSVNDEGRDM 385
           V+ +  +N Q+V E  F +    + +    +  D   GL + Y + ++F+  +  E RD+
Sbjct: 368 VRGICTLNGQVVHEDVFPEKFGTLQK----MIKDPITGLVWIYTEKAVFRYHIQKEARDV 427

Query: 386 WKVYLDMKEYTASLAHCRDSLQ-RDQVYLAQAEEALASRDYLRAASFYAKI-NYILSFEE 445
           W++Y++M ++  +  +C+D  +  D V   +AE    ++ YL +A  YA   NY   FEE
Sbjct: 428 WQMYMNMNKFDLAKEYCKDRPECLDMVLAKEAEHCFQNKRYLESAKCYALTQNY---FEE 487

Query: 446 ITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASD 505
           I LKFI A +++AL+ FL++KL NL   +K QIT++ TW TELYL+++ +L  D     +
Sbjct: 488 IALKFIEAKQEEALKEFLIKKLVNLKPSEKTQITLLVTWLTELYLNRLGQLEAD-----E 547

Query: 506 GNSTDYQSIIKEFRAFLSDSKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVV 565
           G    +    +EFR FL   K       +  T   LL S+G V+ +V+F+ + + YE V+
Sbjct: 548 GKQHLFLETREEFRTFLKSPKHKDCFYNNRSTIYDLLASHGDVDNMVYFSVIMQDYERVI 607

Query: 566 HHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSN-LNPRKLIPA 625
            HY Q  +   AL+VL K      L YKF+P L+     + V++W+   N L+P+ LIPA
Sbjct: 608 SHYCQHDDYSAALDVLSK-HCDDKLFYKFSPVLMQHIPKKVVDAWIQMGNRLDPKNLIPA 667

Query: 626 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 685
           ++ YS +  +  + +E I+Y+E+CV+ L  ++  +HN LLSLYAK + D ALL +L+   
Sbjct: 668 LVNYS-QMGSMQQINETIRYMEFCVYELDVKEEAIHNYLLSLYAKHKPD-ALLWYLE--- 727

Query: 686 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 745
            +   +  D  YD KYALRLC +   ++ACV +Y +M ++EEAV LAL+VD +LA + AD
Sbjct: 728 -QAGTHVSDIHYDLKYALRLCSEHGYLQACVLVYKIMELYEEAVDLALKVDVDLAKSCAD 787

Query: 746 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 805
             EDDE+LRKKLWL IA+HV++ EK     +++KA+  L   + LLKIEDILPFFPDF  
Sbjct: 788 LPEDDEELRKKLWLKIARHVVQEEK-----DVKKAMNCLSSCN-LLKIEDILPFFPDFVT 847

Query: 806 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 865
           ID FKEAICSSLE+YNK I++LKQEM +AT  A  IR+DI  +  +Y +++  E C  C 
Sbjct: 848 IDHFKEAICSSLEEYNKHIEELKQEMEEATESAKRIREDIQEMRNKYGVVESQEKCATCD 907

Query: 866 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 925
             +L                   PFY+F CGH FH  CL+  V       +   + +LQK
Sbjct: 908 FPLLN-----------------RPFYLFLCGHMFHYDCLLQEVIPHLSVYKQNKLDELQK 967

Query: 926 QITLLGGETRRESNGSFAEDSIS---SITPADKLRTQLDDAIAGECPFCGELMIREISLP 965
           ++     +T +  +    ED++S        +++++ +DD IA EC +CGELMI+ I  P
Sbjct: 968 KLAAT-TQTTKARHKPREEDTVSLGKGQGSREQIKSDIDDIIACECVYCGELMIKSIDKP 973

BLAST of CmoCh19G009950 vs. ExPASy Swiss-Prot
Match: Q8R307 (Vacuolar protein sorting-associated protein 18 homolog OS=Mus musculus OX=10090 GN=Vps18 PE=1 SV=2)

HSP 1 Score: 480.3 bits (1235), Expect = 5.1e-134
Identity = 328/963 (34.06%), Postives = 508/963 (52.75%), Query Frame = 0

Query: 26  CMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSVGRPGEQSIHRIFVDPGGSHCITTVV 85
           CMS G D +L         R D G   S    + +GR  +  +H++F+D  GSH +   V
Sbjct: 68  CMSLGKDTLL---------RIDLGKA-SEPNRVELGRKDDAKVHKMFLDHTGSHLL---V 127

Query: 86  GAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFELAV 145
             +  +  YM+    K R L R KG +V +V WN+    E+ST  +++GT  GQ+FE  +
Sbjct: 128 ALSSTEVLYMNRNGQKARPLARWKGQLVESVGWNKAMGNESSTGPILVGTAQGQIFEAEL 187

Query: 146 DEKE------KKEKYVKFLFELAELPEAFMDLQMETTSVLNGMRFYVMAVTPTRLYSFTG 205
              E        + Y + L+ L E         +E     +G R +V+A T  RL+ F G
Sbjct: 188 SASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDG-RGFVIATTRQRLFQFIG 247

Query: 206 --IGSLE----MGLYTS-LEHYAPY------LPSSELHFYIKQRRA--MHFAWLSGAGIY 265
             +   E     GL+ +  +H  P+      L  SEL FY  + R+    FAW+ G G+ 
Sbjct: 248 RAVEDTEAQGFAGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVL 307

Query: 266 HGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHFLLLIGNKVKV 325
           +G L+ G         ++ +  + + +Y       G+  P ++ +++FHFLLL+ ++V+ 
Sbjct: 308 YGSLDCGR-------PDSLLSEERVWEY-PAGVGPGANPPLAIVLTQFHFLLLLADRVEA 367

Query: 326 VNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSVNDEGRDMWKV 385
           V  +  Q+V    F +    +      +  D++ G  +AY + ++F+  V  E RD+W+ 
Sbjct: 368 VCTLTGQVVLRDHFLEKFGPLRH----MVKDSSTGHLWAYTERAVFRYHVQREARDVWRT 427

Query: 386 YLDMKEYTASLAHCRDSLQ-RDQVYLAQAEEALASRDYLRAASFYAKINYILSFEEITLK 445
           YLDM  +  +  +CR+     D V   +A+       YL +A  YA       FEEI LK
Sbjct: 428 YLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQHRYLESARCYALTQSY--FEEIALK 487

Query: 446 FISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASDGNST 505
           F+ A +++AL  FL RKL  L   ++ Q T+++TW TELYL ++  L  D D       T
Sbjct: 488 FLEARQEEALAEFLQRKLAGLKPTERTQATLLTTWLTELYLSRLGALQGDPDAL-----T 547

Query: 506 DYQSIIKEFRAFLSDSKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYI 565
            Y+   + FR FLS  +          +  +LL S+G  E +V+FA + + YE VV ++ 
Sbjct: 548 LYRDTRECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHC 607

Query: 566 QQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWM-ITSNLNPRKLIPAMMRY 625
           Q    ++AL VL +   P  L YKF+P LI     + V++W+ + S L+ R+LIPA++ Y
Sbjct: 608 QHEAYEEALAVLARHRDP-QLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNY 667

Query: 626 SGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGL 685
           S    A+ +  + I+Y+E+CV+ L   +  +HN LLSLYA+ +  ++LL +L+    +  
Sbjct: 668 SQGGEAQ-QVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQ-PASLLAYLE----QAG 727

Query: 686 ENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEADKVED 745
            +     YD KYALRLC +    RACVH+Y ++ ++EEAV LALQVD +LA   AD  E+
Sbjct: 728 ASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEE 787

Query: 746 DEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDF 805
           DE+LRKKLWL IA+HV++ E     E+++ A+A L     LLKIED+LPFFPDF  ID F
Sbjct: 788 DEELRKKLWLKIARHVVQEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHF 847

Query: 806 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCKRKIL 865
           KEAICSSL+ YN  I +L++EM +AT  A  IR+D+  L  RY  ++  + C  C   +L
Sbjct: 848 KEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTCDFPLL 907

Query: 866 TVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITL 925
                              PFY+F CGH FHA CL+  V       +   + +LQ+++  
Sbjct: 908 N-----------------RPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGA 967

Query: 926 LGGETRRESNGSFAEDSISSITPA-DKLRTQLDDAIAGECPFCGELMIREISLPFISSEE 961
               T+       AE   +++ P+ ++L+  LD+ +A EC +CGELMIR I  PFI  + 
Sbjct: 968 APPPTKGSVKAKEAEAGAAAVGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQR 967

BLAST of CmoCh19G009950 vs. ExPASy Swiss-Prot
Match: Q9P253 (Vacuolar protein sorting-associated protein 18 homolog OS=Homo sapiens OX=9606 GN=VPS18 PE=1 SV=2)

HSP 1 Score: 479.9 bits (1234), Expect = 6.7e-134
Identity = 327/967 (33.82%), Postives = 512/967 (52.95%), Query Frame = 0

Query: 26  CMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSVGRPGEQSIHRIFVDPGGSHCITTVV 85
           CMS G D +L         R D G  +  ++ + +GR  +  +H++F+D  GSH +   +
Sbjct: 68  CMSLGKDTLL---------RIDLGKANEPNH-VELGRKDDAKVHKMFLDHTGSHLL---I 127

Query: 86  GAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNRQHITEASTKEVILGTDNGQLFELAV 145
             +  +  Y++    K R L R KG +V +V WN+   TE+ST  +++GT  G +FE  +
Sbjct: 128 ALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAEL 187

Query: 146 DEKE------KKEKYVKFLFELAELPEAFMDLQMETTSVLNGMRFYVMAVTPTRLYSFTG 205
              E        + Y + L+ L E         +E     +G R +V+A T  RL+ F G
Sbjct: 188 SASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDG-RSFVIATTRQRLFQFIG 247

Query: 206 IGSLE------MGLYTS-LEHYAPY------LPSSELHFYIKQRRA--MHFAWLSGAGIY 265
             +         GL+ +  +H  P+      L  SEL FY  + R+    FAW+ G G+ 
Sbjct: 248 RAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVL 307

Query: 266 HGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHFLLLIGNKVKV 325
           +G L+ G         ++ +  + + +Y +     G+  P ++ +++FHFLLL+ ++V+ 
Sbjct: 308 YGALDCGR-------PDSLLSEERVWEYPE-GVGPGASPPLAIVLTQFHFLLLLADRVEA 367

Query: 326 VNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSVNDEGRDMWKV 385
           V  +  Q+V    F +    +      +  D++ G  +AY + ++F+  V  E RD+W+ 
Sbjct: 368 VCTLTGQVVLRDHFLEKFGPLKH----MVKDSSTGQLWAYTERAVFRYHVQREARDVWRT 427

Query: 386 YLDMKEYTASLAHCRDSLQ-RDQVYLAQAEEALASRDYLRAASFYAKINYILSFEEITLK 445
           YLDM  +  +  +CR+     D V   +A+     R YL +A  YA       FEEI LK
Sbjct: 428 YLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSY--FEEIALK 487

Query: 446 FISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDDDIASDGNST 505
           F+ A +++AL  FL RKL +L   ++ Q T+++TW TELYL ++  L  D +       T
Sbjct: 488 FLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEAL-----T 547

Query: 506 DYQSIIKEFRAFLSDSKD----VLDEVTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYI 565
            Y+   + FR FLS  +          +  +LL S+G  E +V+FA + + YE VV ++ 
Sbjct: 548 LYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHC 607

Query: 566 QQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWM-ITSNLNPRKLIPAMMRY 625
           Q    ++AL VL +   P  L YKF+P LI     + V++W+ + S L+ R+LIPA++ Y
Sbjct: 608 QHEAYEEALAVLARHRDP-QLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNY 667

Query: 626 SGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGL 685
           S +     +  + I+Y+E+CV+ L   +  +HN LLSLYA+   DS LL +L+    +  
Sbjct: 668 S-QGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDS-LLAYLE----QAG 727

Query: 686 ENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEADKVED 745
            +     YD KYALRLC +    RACVH+Y ++ ++EEAV LALQVD +LA   AD  E+
Sbjct: 728 ASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEE 787

Query: 746 DEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDF 805
           DE+LRKKLWL IA+HV++ E     E+++ A+A L     LLKIED+LPFFPDF  ID F
Sbjct: 788 DEELRKKLWLKIARHVVQEE-----EDVQTAMACLASCP-LLKIEDVLPFFPDFVTIDHF 847

Query: 806 KEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCKRKIL 865
           KEAICSSL+ YN  I +L++EM +AT  A  IR+D+  L  RY  ++  + C  C   +L
Sbjct: 848 KEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLL 907

Query: 866 TVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITL 925
                              PFY+F CGH FHA CL+  V       +   + +LQ+++  
Sbjct: 908 N-----------------RPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGA 967

Query: 926 LGGETRRESNGSFAEDSISSITPA-DKLRTQLDDAIAGECPFCGELMIREISLPFISSE- 963
                +  +    AE   ++  P+ ++L+  LD+ +A EC +CGELMIR I  PFI  + 
Sbjct: 968 APPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQR 971

BLAST of CmoCh19G009950 vs. ExPASy Swiss-Prot
Match: Q24314 (Vacuolar protein sorting-associated protein 18 homolog OS=Drosophila melanogaster OX=7227 GN=dor PE=1 SV=3)

HSP 1 Score: 328.6 bits (841), Expect = 2.5e-88
Identity = 268/945 (28.36%), Postives = 447/945 (47.30%), Query Frame = 0

Query: 68   IHRIFVDPGGSHCITTVV-----GAAGADTFYMH------AKWSKPRILPRLKGLVVNTV 127
            I R+F+DP G H I  +V          D  Y+H      A+  K R + + K   +  V
Sbjct: 111  ITRMFLDPTGHHIIIALVPKSATAGVSPDFLYIHCLESPQAQQLKVRRIEKFKDHEITAV 170

Query: 128  AWNRQHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETT 187
            A+N  H  E+ST  ++LGT  G +FE  ++         K L++L      +    ++  
Sbjct: 171  AFNPYHGNESSTGPILLGTSRGLIFETELNPAADGHVQRKQLYDLGLGRPKYPITGLKLL 230

Query: 188  SVLNGMRFYVMAVTPTRLYSF--------TGIGSLEMGLYTSL-----EHYAPYLPSSEL 247
             V N  R+ ++  +P  +Y+F          + ++  G  + +     E     L  S+L
Sbjct: 231  RVPNSSRYIIVVTSPECIYTFQETLKAEERSLQAIFAGYVSGVQEPHCEERKTDLTFSQL 290

Query: 248  HFYI--KQRRAMHFAWLSGAGIYHGELNFGSQHSSSNGDENFVENKAL-LDYSKLTENSG 307
             F+     +    +AWL G GI  GEL+       +N     + N  + LD+ K    S 
Sbjct: 291  RFFAPPNSKYPKQWAWLCGEGIRVGELSI-----EANSAATLIGNTLINLDFEKTMHLSY 350

Query: 308  SVK----PSSLAVSEFHFLLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDA 367
              +    P +  ++E+H +LL  + V+ +  +N++ V +  FD+     P   L +  D 
Sbjct: 351  GERRLNTPKAFVLTEYHAVLLYADHVRAICLLNQEQVYQEAFDEARVGKP---LSIERDE 410

Query: 368  TAGLFYAYDQNSIFQVSVNDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQ-AEEA 427
              G  Y Y   ++F + V  E R++W++YLD  +Y  + AH  +  +  Q+ L Q A+ A
Sbjct: 411  LTGSIYVYTVKTVFNLRVTREERNVWRIYLDKGQYELATAHAAEDPEHLQLVLCQRADAA 470

Query: 428  LASRDYLRAASFYAKINYILSFEEITLKFISASEQDALRTFLLRKL----------DNLT 487
             A   Y  AA +YA+ +   SFEE+ LKF+   ++  +  ++ ++L          D L 
Sbjct: 471  FADGSYQVAADYYAETD--KSFEEVCLKFMVLPDKRPIINYVKKRLSRVTTKPMETDELD 530

Query: 488  KDDKCQITMISTWATELYLDKINRLLLDDDIASDGNSTDYQSIIKEFRAFLSDSKDVLDE 547
            +D    I  +  W  +LYL +IN    D++  S    T+Y   + E    LS ++   + 
Sbjct: 531  EDKMNIIKALVIWLIDLYLIQINMPDKDEEWRSSW-QTEYDEFMMEAHV-LSCTRQ--NR 590

Query: 548  VTTMKLLESYGRVEELVFFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPADLQYKFA 607
             T  +L+  +     +  FA     Y+ VV   ++     +AL+ L     P +L YK+A
Sbjct: 591  ETVRQLIAEHADPRNMAQFAIAIGDYDEVVAQQLKAECYAEALQTLINQRNP-ELFYKYA 650

Query: 608  PELIMLDAYETVESWMIT-SNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHN 667
            PELI      TV++ M   S L   KL+P ++        + +  +  +YLE+ +++L+ 
Sbjct: 651  PELITRLPKPTVDALMAQGSRLEVEKLVPTLIIMEN----REQREQTQRYLEFAIYKLNT 710

Query: 668  EDPGVHNLLLSLYAKQEDDSALLRFLQCKFGKGLENGPD---FFYDPKYALRLCLKEKRM 727
             +  +HN LL LYA+ E    L+++L+ +       G D     YD  YA ++C      
Sbjct: 711  TNDAIHNFLLHLYAEHE-PKLLMKYLEIQ-------GRDESLVHYDIYYAHKVCTDLDVK 770

Query: 728  RACVHIYSMMAMHEEAVALALQVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGT 787
             A V +  M+     AV LAL  D +LA   A +  D + +R+KLWL IA H I   KGT
Sbjct: 771  EARVFLECMLRKWISAVDLALTFDMKLAKETASRPSDSK-IRRKLWLRIAYHDI---KGT 830

Query: 788  KRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIDQLKQEMN 847
               +++KA+  LKE D LL+IED+LPFF DF  ID+FKEAIC +L DYN++I +L++EM 
Sbjct: 831  --NDVKKALNLLKECD-LLRIEDLLPFFADFEKIDNFKEAICDALRDYNQRIQELQREMA 890

Query: 848  DATHGADNIRKDINALAQRYAIIDRDEDCGVCKRKILTVGRDYLMASSYTSVAQMAPFYV 907
            + T   D +  ++  L Q    ++  + C +C+  +L                 + PF++
Sbjct: 891  ETTEQTDRVTAELQQLRQHSLTVESQDTCEICEMMLL-----------------VKPFFI 950

Query: 908  FPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQKQITLLGGETRRESNGSFAEDSISSI-T 966
            F CGH FH+ CL  HV     + Q   +  L++Q+        +  +G+ ++     +  
Sbjct: 951  FICGHKFHSDCLEKHVVPLLTKEQCRRLGTLKQQLEAEVQTQAQPQSGALSKQQAMELQR 1002

BLAST of CmoCh19G009950 vs. ExPASy TrEMBL
Match: A0A6J1GKZ1 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454916 PE=3 SV=1)

HSP 1 Score: 1923.3 bits (4981), Expect = 0.0e+00
Identity = 969/987 (98.18%), Postives = 972/987 (98.48%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. ExPASy TrEMBL
Match: A0A6J1HZJ9 (vacuolar protein sorting-associated protein 18 homolog OS=Cucurbita maxima OX=3661 GN=LOC111468106 PE=3 SV=1)

HSP 1 Score: 1911.7 bits (4951), Expect = 0.0e+00
Identity = 962/987 (97.47%), Postives = 968/987 (98.07%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITT+VGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTIVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI LKFISA+EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEIALKFISANEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DI SDG+STDYQSIIKEFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIVSDGHSTDYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. ExPASy TrEMBL
Match: A0A0A0K5N6 (Pep3_Vps18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046700 PE=3 SV=1)

HSP 1 Score: 1846.2 bits (4781), Expect = 0.0e+00
Identity = 923/989 (93.33%), Postives = 957/989 (96.76%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           M+ GRPAFTVDLLERYAAKGRGVISCM+AGNDVI+LGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPG+QSIHR+FVDPGGSHCITT+VG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGDQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTS+LN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSILN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMR+YVMAVTPTRLYSFTG GSLE    T LE    +      +P+SELHFYIKQRRA+H
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ S SNGDENFVENKALLDYSKLTENSG+VKPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAE+ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DG+ST+YQSII+EFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPA+LQYKFAPELIMLDAYETVESWMIT+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD  M SSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMTSSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISS--ITPADKLRTQLDDAIAGECPFCGELMIREISLPF 960
           QITLLGGETR++SNGSFAEDSISS  +TPADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMTPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

Query: 961 ISSEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           ISSEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 ISSEEAQQVSSWEIRPHNLGGQRSFSLPA 989

BLAST of CmoCh19G009950 vs. ExPASy TrEMBL
Match: A0A1S3C0V2 (LOW QUALITY PROTEIN: vacuolar protein sorting-associated protein 18 homolog OS=Cucumis melo OX=3656 GN=LOC103495539 PE=3 SV=1)

HSP 1 Score: 1838.9 bits (4762), Expect = 0.0e+00
Identity = 921/989 (93.12%), Postives = 955/989 (96.56%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           M+ GRPAFTVDLLERYAAKGRGVISCM+AGNDVI+LGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHR+FVDPGGSHCITT+VG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEK KKEKYVKFLFELAELPEAFMDLQMETT+VLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKXKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMR+YVMAVTPTRLYSFTG GSLE    T LE    +      +P+SELHFYIKQRRA+H
Sbjct: 181 GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ S SNGDENFVENKALLDYSKLTENSG+VKPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTVKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAE+ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI+LKFISA EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEISLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           D A DG+ST+YQSII+EFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGV A+LQYKFAPELIMLDAYETVESWMIT+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVLAELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD  MASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSIT--PADKLRTQLDDAIAGECPFCGELMIREISLPF 960
           QITLLGGETR++SNGSFAEDSISS+T  PADKLRTQLDDAIAGECPFCGELMIREISLPF
Sbjct: 901 QITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFCGELMIREISLPF 960

Query: 961 ISSEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           ISSEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 ISSEEAQQVSSWEIRPHNLGGQRSFSLPA 989

BLAST of CmoCh19G009950 vs. ExPASy TrEMBL
Match: A0A5A7SQQ7 (Vacuolar protein sorting-associated protein 18-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003670 PE=3 SV=1)

HSP 1 Score: 1838.2 bits (4760), Expect = 0.0e+00
Identity = 925/1001 (92.41%), Postives = 956/1001 (95.50%), Query Frame = 0

Query: 1    MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
            M+ GRPAFTVDLLERYAAKGRGVISCM+AGNDVI+LGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1    MEQGRPAFTVDLLERYAAKGRGVISCMAAGNDVIMLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61   GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
            GRPGEQSIHR+FVDPGGSHCITT+VG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61   GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLARLKGLVVNTVAWNR 120

Query: 121  QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
            QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETT+VLN
Sbjct: 121  QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTNVLN 180

Query: 181  GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY--LPS----------------SE 240
            GMR+YVMAVTPTRLYSFTG GSLE    T LE    +  LP                 SE
Sbjct: 181  GMRYYVMAVTPTRLYSFTGTGSLETVFSTYLERAVHFMELPGEIPNRQCYQFRRLIHYSE 240

Query: 241  LHFYIKQRRAMHFAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSV 300
            LHFYIKQRRA+HFAWLSGAGIYHGELNFGSQ S SNGDENFVENKALLDYSKLTENSG+V
Sbjct: 241  LHFYIKQRRAIHFAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTV 300

Query: 301  KPSSLAVSEFHFLLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFY 360
            KPSS+AVSEFHFLLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATAGLFY
Sbjct: 301  KPSSMAVSEFHFLLLIGNKVKVVNRISEQIIEELQFDQTSEAITRGILGLCSDATAGLFY 360

Query: 361  AYDQNSIFQVSVNDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYL 420
            AYDQNSIFQVSVNDEGRDMWKVYLDMKEYTA+LA+CRDSLQRDQVYLAQAE+ALASRDYL
Sbjct: 361  AYDQNSIFQVSVNDEGRDMWKVYLDMKEYTAALANCRDSLQRDQVYLAQAEDALASRDYL 420

Query: 421  RAASFYAKINYILSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480
            RAASFYAKINYILSFEEITLKFISA EQDALRTFLLRKLDNLTKDDKCQITMISTWATEL
Sbjct: 421  RAASFYAKINYILSFEEITLKFISAGEQDALRTFLLRKLDNLTKDDKCQITMISTWATEL 480

Query: 481  YLDKINRLLLDDDIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELV 540
            YLDKINRLLLDDD A DG+ST+YQSII+EFRAFLSDSKDVLDEVTTMKLLESYGRVEELV
Sbjct: 481  YLDKINRLLLDDDTAFDGHSTEYQSIIQEFRAFLSDSKDVLDEVTTMKLLESYGRVEELV 540

Query: 541  FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMI 600
            FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPA+LQYKFAPELIMLDAYETVESWMI
Sbjct: 541  FFAGLKEQYEIVVHHYIQQGEAKKALEVLQKPGVPAELQYKFAPELIMLDAYETVESWMI 600

Query: 601  TSNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660
            T+NLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED
Sbjct: 601  TNNLNPRKLIPAMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQED 660

Query: 661  DSALLRFLQCKFGKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720
            DSALLRFLQCKFGKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL
Sbjct: 661  DSALLRFLQCKFGKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALAL 720

Query: 721  QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780
            QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI
Sbjct: 721  QVDTELAMAEADKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKI 780

Query: 781  EDILPFFPDFALIDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA 840
            EDILPFFPDFALIDDFKEAIC+SLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA
Sbjct: 781  EDILPFFPDFALIDDFKEAICTSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA 840

Query: 841  IIDRDEDCGVCKRKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTD 900
            +IDRDEDCGVCKRKILTVGRD  MASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTD
Sbjct: 841  VIDRDEDCGVCKRKILTVGRDLWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTD 900

Query: 901  EAQAEYILDLQKQITLLGGETRRESNGSFAEDSISSIT--PADKLRTQLDDAIAGECPFC 960
            EAQAEYILDLQKQITLLGGETR++SNGSFAEDSISS+T  PADKLRTQLDDAIAGECPFC
Sbjct: 901  EAQAEYILDLQKQITLLGGETRKDSNGSFAEDSISSMTMSPADKLRTQLDDAIAGECPFC 960

Query: 961  GELMIREISLPFISSEEAQQVSSWEIRPHNLGGQRSFSLPA 982
            GELMIREISLPFISSEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961  GELMIREISLPFISSEEAQQVSSWEIRPHNLGGQRSFSLPA 1001

BLAST of CmoCh19G009950 vs. NCBI nr
Match: XP_022952155.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita moschata])

HSP 1 Score: 1923.3 bits (4981), Expect = 0.0e+00
Identity = 969/987 (98.18%), Postives = 972/987 (98.48%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. NCBI nr
Match: KAG6572340.1 (Vacuolar sorting protein 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1921.4 bits (4976), Expect = 0.0e+00
Identity = 968/987 (98.07%), Postives = 972/987 (98.48%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDG+STDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGHSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. NCBI nr
Match: XP_022968943.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita maxima])

HSP 1 Score: 1911.7 bits (4951), Expect = 0.0e+00
Identity = 962/987 (97.47%), Postives = 968/987 (98.07%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITT+VGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTIVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEI LKFISA+EQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEIALKFISANEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DI SDG+STDYQSIIKEFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIVSDGHSTDYQSIIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. NCBI nr
Match: XP_038887715.1 (vacuolar sorting protein 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 1854.7 bits (4803), Expect = 0.0e+00
Identity = 924/987 (93.62%), Postives = 958/987 (97.06%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           M+PGRPAFTVDLLERYAAKGRGVI+CM+AGNDVILLGTSKGWVTRYDFG+GDSID+DLSV
Sbjct: 1   MEPGRPAFTVDLLERYAAKGRGVITCMAAGNDVILLGTSKGWVTRYDFGVGDSIDFDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHR+FVDPGGSHCITT+VG  GADTFYMHAKWSKPR+L RLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRVFVDPGGSHCITTIVGTGGADTFYMHAKWSKPRLLTRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMR+YVMAVTPTRLYSFTG+GSLE    T LE    +      +P+SELHFYIKQRRA+H
Sbjct: 181 GMRYYVMAVTPTRLYSFTGLGSLETVFSTYLERAVHFMELPGEIPNSELHFYIKQRRAIH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQ S SNGDENFVENKALLDYSKLTENSG++KPSS+AVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQRSLSNGDENFVENKALLDYSKLTENSGTLKPSSMAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRI+EQI+EELQFDQT+EAI RGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRISEQIIEELQFDQTSEAISRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTA+LA+CRD+LQRDQVYLAQAE ALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTAALANCRDALQRDQVYLAQAEGALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDG+ST+YQS+IKEFRAFLSD KDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV
Sbjct: 481 DIASDGHSTEYQSVIKEFRAFLSDCKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
           VHHYIQQGEAKKALEVLQKPG P +LQYKFAPELIMLDAYETVESWMIT+NLNPRKLIPA
Sbjct: 541 VHHYIQQGEAKKALEVLQKPGAPPELQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKG ENGP+FFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGQENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYA+IDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAVIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRD+ MASSYTSVA MAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDFWMASSYTSVAHMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETR++ NGSFAEDSISS+TPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRKDLNGSFAEDSISSMTPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 987

BLAST of CmoCh19G009950 vs. NCBI nr
Match: XP_023511650.1 (vacuolar protein sorting-associated protein 18 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1854.3 bits (4802), Expect = 0.0e+00
Identity = 941/987 (95.34%), Postives = 951/987 (96.35%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV
Sbjct: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GRPGEQSIHRIFVDPGGSHCITT+VGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR
Sbjct: 61  GRPGEQSIHRIFVDPGGSHCITTIVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN
Sbjct: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY------LPSSELHFYIKQRRAMH 240
           GMRFYVMAVTPTRLYSFTGIGSLE    T LE    +      +P+SELHFYIKQRRAMH
Sbjct: 181 GMRFYVMAVTPTRLYSFTGIGSLETVFSTYLERAVHFMELPGDIPNSELHFYIKQRRAMH 240

Query: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300
           FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF
Sbjct: 241 FAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFHF 300

Query: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360
           LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV
Sbjct: 301 LLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVSV 360

Query: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420
           NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI
Sbjct: 361 NDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINYI 420

Query: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480
           LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD
Sbjct: 421 LSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLDD 480

Query: 481 DIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEIV 540
           DIASDG+STDYQSIIKEFRAFLSDSKDVLDEVTTMKLLE   R   L+            
Sbjct: 481 DIASDGHSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLE---RCSILLSAKN-------- 540

Query: 541 VHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIPA 600
            + ++ QGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMIT+NLNPRKLIPA
Sbjct: 541 -YCFMDQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITNNLNPRKLIPA 600

Query: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660
           MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF
Sbjct: 601 MMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCKF 660

Query: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720
           GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD
Sbjct: 661 GKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEAD 720

Query: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780
           KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL
Sbjct: 721 KVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFAL 780

Query: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840
           IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK
Sbjct: 781 IDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVCK 840

Query: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900
           RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK
Sbjct: 841 RKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQK 900

Query: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960
           QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS
Sbjct: 901 QITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFIS 960

Query: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 982
           SEEAQQVSSWEIRPHNLGGQRSFSLPA
Sbjct: 961 SEEAQQVSSWEIRPHNLGGQRSFSLPA 975

BLAST of CmoCh19G009950 vs. TAIR 10
Match: AT1G12470.1 (zinc ion binding )

HSP 1 Score: 1547.3 bits (4005), Expect = 0.0e+00
Identity = 754/988 (76.32%), Postives = 863/988 (87.35%), Query Frame = 0

Query: 1   MDPGRPAFTVDLLERYAAKGRGVISCMSAGNDVILLGTSKGWVTRYDFGMGDSIDYDLSV 60
           MD GR  F+VDLLERYA K RG+I+CM+AGNDVI+LGTSKGW+ RYDFG+G S D DL+V
Sbjct: 1   MDQGRQVFSVDLLERYATKNRGMITCMAAGNDVIVLGTSKGWIIRYDFGVGSSNDIDLAV 60

Query: 61  GRPGEQSIHRIFVDPGGSHCITTVVGAAGADTFYMHAKWSKPRILPRLKGLVVNTVAWNR 120
           GR GEQSIH++FVDPGGSHCI TV G  GA+TFY HAKW KPR+L RLKGL+VN VAWNR
Sbjct: 61  GRTGEQSIHKVFVDPGGSHCIATVTGVGGAETFYTHAKWLKPRVLSRLKGLLVNAVAWNR 120

Query: 121 QHITEASTKEVILGTDNGQLFELAVDEKEKKEKYVKFLFELAELPEAFMDLQMETTSVLN 180
           Q ITE STKE+ILGT +GQLFE+AVDEK+K+EKY+KFLFEL ELPEAF  LQMET ++ +
Sbjct: 121 QQITEVSTKEIILGTQDGQLFEMAVDEKDKREKYIKFLFELEELPEAFKALQMETANISS 180

Query: 181 GMRFYVMAVTPTRLYSFTGIGSLEMGLYTSLEHYAPY-------LPSSELHFYIKQRRAM 240
           GMR+YVMAVTPTRLYSFTGIG+LE  ++ S +  A +       +P+SELHF+IKQRRA+
Sbjct: 181 GMRYYVMAVTPTRLYSFTGIGTLE-SVFASYKERAVHFMELPGEIPNSELHFFIKQRRAV 240

Query: 241 HFAWLSGAGIYHGELNFGSQHSSSNGDENFVENKALLDYSKLTENSGSVKPSSLAVSEFH 300
           HFAWLSG GIYHG LNFG+QHS  NGDENFVENKALLDYSKL++ + +VKP S+A+SE+H
Sbjct: 241 HFAWLSGTGIYHGGLNFGAQHSYPNGDENFVENKALLDYSKLSDGTEAVKPGSMALSEYH 300

Query: 301 FLLLIGNKVKVVNRINEQIVEELQFDQTAEAIPRGILGLCSDATAGLFYAYDQNSIFQVS 360
           FLLLIGNKVKVVNRI+EQI+EELQFD T++++ RGI+GLCSDA+A +FYAYDQNSIFQVS
Sbjct: 301 FLLLIGNKVKVVNRISEQIIEELQFDITSDSVSRGIIGLCSDASANVFYAYDQNSIFQVS 360

Query: 361 VNDEGRDMWKVYLDMKEYTASLAHCRDSLQRDQVYLAQAEEALASRDYLRAASFYAKINY 420
           V DEGRDMWKVYLD+K Y A+LA+CRD LQRDQVYL QAE A   ++YLRAASFYAKINY
Sbjct: 361 VIDEGRDMWKVYLDLKVYAAALANCRDPLQRDQVYLVQAESAFTDKEYLRAASFYAKINY 420

Query: 421 ILSFEEITLKFISASEQDALRTFLLRKLDNLTKDDKCQITMISTWATELYLDKINRLLLD 480
           ++SFEE+TLKFIS +E +ALRTFLL KLDNL+KDDKCQITMISTWATELYLDKINRLLL+
Sbjct: 421 VISFEEVTLKFISINEPEALRTFLLHKLDNLSKDDKCQITMISTWATELYLDKINRLLLE 480

Query: 481 DDIASDGNSTDYQSIIKEFRAFLSDSKDVLDEVTTMKLLESYGRVEELVFFAGLKEQYEI 540
           DD A +   ++Y S+I+EFRAF+SD KD LDE TT+K+LESYGRVEELV+FA LKEQYEI
Sbjct: 481 DDTAIENRDSEYHSVIQEFRAFMSDCKDELDEATTVKILESYGRVEELVYFANLKEQYEI 540

Query: 541 VVHHYIQQGEAKKALEVLQKPGVPADLQYKFAPELIMLDAYETVESWMITSNLNPRKLIP 600
           VV HYIQQGEAKKALEVLQK  V  +LQY+FAPELIMLDAYETVESWM   NLNPR+LI 
Sbjct: 541 VVLHYIQQGEAKKALEVLQKSSVSVELQYQFAPELIMLDAYETVESWMANKNLNPRRLIT 600

Query: 601 AMMRYSGEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYAKQEDDSALLRFLQCK 660
           AMMRYS  PHAKNETHEVIKYLE+CVHRLHNEDPG+H+LLLSLYAKQEDD ALLRFLQCK
Sbjct: 601 AMMRYSSGPHAKNETHEVIKYLEFCVHRLHNEDPGIHSLLLSLYAKQEDDGALLRFLQCK 660

Query: 661 FGKGLENGPDFFYDPKYALRLCLKEKRMRACVHIYSMMAMHEEAVALALQVDTELAMAEA 720
           FGKG ENGP+FFYDPKYALRLCLKE+R RACVHIYSMM+MHEEAVALALQ+D ELAMAEA
Sbjct: 661 FGKGRENGPEFFYDPKYALRLCLKERRTRACVHIYSMMSMHEEAVALALQIDPELAMAEA 720

Query: 721 DKVEDDEDLRKKLWLMIAKHVIELEKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFA 780
           DKVEDDEDLRKKLWLM+AKHV++ EKG KRENIRKAIAFLKETDGLLKIEDILPFFPDFA
Sbjct: 721 DKVEDDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKETDGLLKIEDILPFFPDFA 780

Query: 781 LIDDFKEAICSSLEDYNKQIDQLKQEMNDATHGADNIRKDINALAQRYAIIDRDEDCGVC 840
           LIDDFKEAICSSLEDYNKQI+QLK+EMNDAT GADNIR DI+AL QRYA+IDRDE+CGVC
Sbjct: 781 LIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISALTQRYAVIDRDEECGVC 840

Query: 841 KRKILTVGRDYLMASSYTSVAQMAPFYVFPCGHGFHAQCLIAHVTRCTDEAQAEYILDLQ 900
           KRKIL +  D+ MA  Y+S   +APFYVFPCGH FHAQCLI HVT C  E QAE+ILDLQ
Sbjct: 841 KRKILMMSGDFRMAQGYSSAGPLAPFYVFPCGHSFHAQCLITHVTSCAHEEQAEHILDLQ 900

Query: 901 KQITLLGGETRRESNGSFAEDSISSITPADKLRTQLDDAIAGECPFCGELMIREISLPFI 960
           KQ+TLLG ETRR+ NG+ +++ I+S T ADKLR++LDDAIA ECPFCGELMI EI+LPFI
Sbjct: 901 KQLTLLGSETRRDINGNRSDEPITSTTTADKLRSELDDAIASECPFCGELMINEITLPFI 960

Query: 961 SSEEAQQVSSWEIRPH-NLGGQRSFSLP 981
             E++Q  +SW++R   NL  QR+ SLP
Sbjct: 961 KPEDSQYSTSWDLRSETNLANQRTISLP 987