CmoCh17G005930 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh17G005930
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	rRNA N-glycosidase
Location	Cmo_Chr17: 6299793 .. 6300620 (-)
RNA-Seq Expression	CmoCh17G005930
Synteny	CmoCh17G005930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG

mRNA sequence

ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG

Coding sequence (CDS)

ATGAATAGCGTGTCGGTGTTTTGTTTGGTAATTCTGGCCATCTTCCATGGAAGTTCAAGTTGTGTTGCGGGTGACGTGAGCTTCAACCTGTTGGGTTCGAATTCCAAATCCTATAGCAACTTCATCGAAGCCCTGAGGAGTGCTCTTCCATCCAAGGACAAAGTATACAATATATCACTATTGCTTGGCTCGGCAACAGGCGCAAGTCGCTACACACAAGTGAAACTCTCCAATTATGACGGGAAAGCCATCACGGTGGCAATAGATGTAACAAACGTTTACATCATGGGGTATCTGGTGAACTCAACTTCCTACTTCTTCAACGAATCTGATGCCCAATTGGCTTCCAAATATGTATTCAAAGACAGCACCCGTATTACACTTCCATACTCCGGCAATTATGAAAAGCTTCAAAATGCGGCAGGCAAAAAAAGGGAAAAAATTCCCCTTGGATTCCCAGCTTTGGACAATGCAATTACCACCTTGTTTCATTACGACTCCACGGCTGCTGCTGCGGCATTTATCGTGGTGATTCAGTGCACGGCTGAGGCTTCAAGATACAGATACATCGAGGCCCAAATGATTAAGAGAATTTCCAAAGACGATGTCCCAAGTCTGGCGATTATCAGTTTAGAAAACAGCTGGTCTGCTCTCTCCAAACAAATTCAATTGGCTCAAACAAATAAAGGAGTTTTTAAAGCTCCGGTTTTGATTACAGACGATCAAGGCCAACGAGTTGAAATAACCAATGTCAGTTCCAAGGTTGTAGCCAAAAACATCCAGTTGCTACTAAACTCAAAAAATATTGCAGATTTGAGCTACAATTAG

Protein sequence

MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN

Homology

[Close]

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-104
Identity = 206/265 (77.74%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSKVVAKNIQLLLNSK-NIA 271
           EITNV+SKVV KNIQLLLN K N+A
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVA 267

[Close]

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 6.7e-100
Identity = 194/241 (80.50%), Postives = 211/241 (87.55%), Query Frame = 0


Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           DVSF+L GS+S SYS FI ALR ALPS   VYNI+LLL SA+GASRYT +KLSNYDGKAI
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           TVAIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF  ST +TLPYSGNYEKLQ AAGK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAE+SR++YIE Q+I RISK+ VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 205 SLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAKNIQLLL 264
           SLA ISLEN WSALSKQIQLAQTN G FK PV+I D  GQRVEI NV SKVV KNIQLLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

Query: 265 N 266
           N
Sbjct: 241 N 241

[Close]

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.1e-92
Identity = 180/249 (72.29%), Postives = 217/249 (87.15%), Query Frame = 0


Query: 25  DVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSATGASRYTQVKLSNYDGKAI 84
           +VSF+L G++SKSYS FI ALR ALPSK+KV NI LLL SA+GASRY  ++LSNYD KAI
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 85  TVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRITLPYSGNYEKLQNAAGKK 144
           T+AIDVTNVYIMGYLVNSTSYF NESDA+LAS+YVFK ST +T+PYSGNYE+LQNAAGK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 145 REKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASRYRYIEAQMIKRISKDDVP 204
           REKIPLGF ALD+A+T++FHYDSTAAAAAF+V++Q TAEASR++YIE Q+I+RI K++VP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 205 SLAIISLEN-SWSALSKQIQLAQTNKGVFKAPVLITDDQGQRVEITNVSSKVVAK--NIQ 264
           S A +SLEN +WS LSKQIQLAQTN G F+ PV+I D++GQRVEITN++SKV  K  N +
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

Query: 265 LLLNSKNIA 271
           LLLN +NIA
Sbjct: 242 LLLNKQNIA 250

[Close]

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.3e-91
Identity = 178/272 (65.44%), Postives = 221/272 (81.25%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           M+  SV   +ILAIF G  S V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLG-GSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL S +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KDSTR-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQ 180
           +D+ R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 CTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLIT 240
            TAEA+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DDQGQRVEITNVSSKVVAKNIQLLLNSKNIAD 272
           D++G RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAE 271

[Close]

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 172/273 (63.00%), Postives = 214/273 (78.39%), Query Frame = 0


Query: 1   MNSVSV-FCLVILAIFHGSSSCVAGDVS--FNLLGSNSKSYSNFIEALRSALPSKDKVYN 60
           MN  SV  CLVIL+IFHG  +   GDV+  F+LLGSN KSYS FI ++R+ALP+   +YN
Sbjct: 1   MNRFSVLMCLVILSIFHGVPT-AEGDVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYN 60

Query: 61  ISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASK 120
           I LL+ S +G+ RY  ++LSNY+G  IT+A+DVTNVYIMGYLVN TSYFFNE+DAQLASK
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 121 YVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVV 180
           +VF+ +  ITLPYSGNY+KLQ+ A K+R+ IPLGF ALD+AI+TL++YDS +A  AF+V+
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVL 180

Query: 181 IQCTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVL 240
           IQ TAEA+RY+YIE Q+I RIS   VP LA ISLEN WS LSKQIQ+A++N G F+ PV 
Sbjct: 181 IQTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVK 240

Query: 241 ITDDQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           I +D+G   E+TNVSS VV KNI LLLN  NIA
Sbjct: 241 IINDKGILTEVTNVSSLVVTKNIMLLLNKLNIA 272

[Close]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.0e-144
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 285

[Close]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.8e-93
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.8e-93
Identity = 189/270 (70.00%), Postives = 225/270 (83.33%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN  +   L+I+ I     +    +VSF+L G++SKSYS FI ALR ALPSK+KV NI L
Sbjct: 1   MNRFTFLSLLIILI--AFFTVEGANVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL SA+GASRY  ++LSNYD KAIT+AIDVTNVYIMGYLVNSTSYFFNESDA+LAS+YVF
Sbjct: 61  LLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           K ST +TLPYSGNYE+LQNAAGK REKIPLGF A D+AIT+LFHYDSTAAA AF+V+IQ 
Sbjct: 121 KGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVIIQT 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASR++YIE Q+I+RI K++VPS A +SLEN WSALSKQIQLAQTN G F+ PV+I D
Sbjct: 181 TAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVVIID 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIA 271
           ++GQRVEI +V+SKVV  NI+LLLN +NIA
Sbjct: 241 NKGQRVEIKDVNSKVVTNNIKLLLNKQNIA 268

[Close]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL
Match: A0A6J1DSN8 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.2e-88
Identity = 178/272 (65.44%), Postives = 221/272 (81.25%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           M+  SV   +ILAIF G  S V GDVSF L G++ +SY  FI+ LR+ALP ++KVYNI L
Sbjct: 1   MSRFSVLSFLILAIFLG-GSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LL S +GA RY  + L NYDGK ITVA+DVTNVYIMGYL ++TSYFFNE  A+LAS+YVF
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVF 120

Query: 121 KDSTR-ITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQ 180
           +D+ R ITLPYSGNYE+LQ AAGK REKIP+G PALD+AI+TL HYDSTAAA A +V+IQ
Sbjct: 121 RDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQ 180

Query: 181 CTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLIT 240
            TAEA+R++YIE Q+ +R  +D+VPSLA ISLENSWS LSKQIQLAQ N G+F+ P+++ 
Sbjct: 181 TTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLV 240

Query: 241 DDQGQRVEITNVSSKVVAKNIQLLLNSKNIAD 272
           D++G RV+ITNV+SKVV  NIQLLLN++NIA+
Sbjct: 241 DNKGNRVQITNVTSKVVTSNIQLLLNTRNIAE 271

[Close]

BLAST of CmoCh17G005930 vs. NCBI nr
Match: XP_022953306.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata])

HSP 1 Score: 520.0 bits (1338), Expect = 1.2e-143
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmoCh17G005930 vs. NCBI nr
Match: KAG6575376.1 (hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013917.1 hypothetical protein SDJN02_24086, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520.0 bits (1338), Expect = 1.2e-143
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL
Sbjct: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC
Sbjct: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN
Sbjct: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 275

[Close]

BLAST of CmoCh17G005930 vs. NCBI nr
Match: XP_023520860.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 517.7 bits (1332), Expect = 6.2e-143
Identity = 273/275 (99.27%), Postives = 275/275 (100.00%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL
Sbjct: 11  MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           L+GSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LVGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAF+VVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFMVVIQC 190

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN
Sbjct: 251 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 285

[Close]

BLAST of CmoCh17G005930 vs. NCBI nr
Match: XP_022992350.1 (ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima])

HSP 1 Score: 502.3 bits (1292), Expect = 2.7e-138
Identity = 266/275 (96.73%), Postives = 270/275 (98.18%), Query Frame = 0


Query: 1   MNSVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISL 60
           MN VSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFI+ALRSALPSKDKVYNISL
Sbjct: 11  MNVVSVFCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIKALRSALPSKDKVYNISL 70

Query: 61  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 120
           LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF
Sbjct: 71  LLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVF 130

Query: 121 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQC 180
           KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALD+AITTLFHYDSTAAAAAFIVVIQC
Sbjct: 131 KDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIVVIQC 190

Query: 181 TAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 240
           TAEASRY+YIEAQMIKRISKDDVPS AIISLENSWSALSKQIQLAQTNKGVFKAPVLITD
Sbjct: 191 TAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPVLITD 250

Query: 241 DQGQRVEITNVSSKVVAKNIQLLLNSKNIADLSYN 276
           DQGQRVEITNVSS VV KNIQLLLNS+NIA LSYN
Sbjct: 251 DQGQRVEITNVSSNVVTKNIQLLLNSQNIAALSYN 285

[Close]

BLAST of CmoCh17G005930 vs. NCBI nr
Match: Q00465.1 (RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Luffa aegyptiaca] >CAA44229.1 a-luffin [Luffa aegyptiaca])

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-101
Identity = 206/265 (77.74%), Postives = 231/265 (87.17%), Query Frame = 0


Query: 7   FCLVILAIFHGSSSCVAGDVSFNLLGSNSKSYSNFIEALRSALPSKDKVYNISLLLGSAT 66
           F ++ILAIF  +S+ V  DV F+L GS+S SYS FI  LR ALPS   VYNI+LLL SA+
Sbjct: 4   FTVLILAIFVAAST-VEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSAS 63

Query: 67  GASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLASKYVFKDSTRI 126
           GASRYT + LSNYDGKAITVA+DVTNVYIMGYLVNSTSYFFNESDA+LAS+YVFK ST +
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIV 123

Query: 127 TLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIVVIQCTAEASR 186
           TLPYSGNYEKLQ AAGK REKIPLGFPALD+AITTLFHYDSTAAAAAF+V+IQ TAEASR
Sbjct: 124 TLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASR 183

Query: 187 YRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPVLITDDQGQRV 246
           ++YIE Q+I+RISK+ VPSLA ISLEN WSALSKQIQLAQTN G FK PV+ITDD+GQRV
Sbjct: 184 FKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRV 243

Query: 247 EITNVSSKVVAKNIQLLLNSK-NIA 271
           EITNV+SKVV KNIQLLLN K N+A
Sbjct: 244 EITNVTSKVVTKNIQLLLNYKQNVA 267

The following BLAST results are available for this feature:

BLAST of CmoCh17G005930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00465	1.5e-104	77.74	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84530	6.7e-100	80.50	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]
P22851	5.1e-92	72.29	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	3.3e-91	65.44	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q9FRX4	1.6e-85	63.00	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]

BLAST of CmoCh17G005930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMM2	6.0e-144	100.00	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]
A0A6J1JVG7	1.3e-138	96.73	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
B0EVM6	2.8e-93	70.00	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	2.8e-93	70.00	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1DSN8	1.2e-88	65.44	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023551 PE=3 SV=1	[more]

BLAST of CmoCh17G005930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022953306.1	1.2e-143	100.00	ribosome-inactivating protein luffaculin 1-like [Cucurbita moschata]	[more]
KAG6575376.1	1.2e-143	100.00	hypothetical protein SDJN03_26015, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023520860.1	6.2e-143	99.27	ribosome-inactivating protein luffaculin 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022992350.1	2.7e-138	96.73	ribosome-inactivating protein luffaculin 1-like [Cucurbita maxima]	[more]
Q00465.1	1.2e-101	77.74	RecName: Full=Ribosome-inactivating protein luffin-alpha; AltName: Full=rRNA N-g...	[more]

BLAST of CmoCh17G005930 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 145..159 score: 54.39 coord: 174..194 score: 50.0 coord: 36..49 score: 46.24 coord: 83..98 score: 52.3 coord: 206..223 score: 53.65
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..185 e-value: 3.1E-47 score: 162.2
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 186..270 e-value: 3.3E-27 score: 96.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 35..223 e-value: 5.3E-32 score: 111.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 27..269
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 27..269
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 25..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh17G005930.1	CmoCh17G005930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity