CmoCh14G005030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh14G005030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionWAT1-related protein
LocationCmo_Chr14: 2492288 .. 2494833 (-)
RNA-Seq ExpressionCmoCh14G005030
SyntenyCmoCh14G005030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTCCATACCACTCTTTCCCTTTTATACATAACAAAACAAGCATGGGATACTTGCAATCCCTTGGCTGTCTCCTTCAAAATGCAAAGCCTTACATCGTTGTCATCTCCCTCCAATTTGGCTATGGCGGAATGAATATCATCTCTGCCATCTCCCTCAACCACGGTATGAGCCACTACGTTCTCGTTGTCTACCGCCACGCCTTTGCCACAGTCGTCATGGCACCTTTTGCTCTTGTTTTTGAGAGGTATATATCTTTGAGTTCGTACTTATCATATTCTTTAACGAGTAACTCATTTCTAGTTTCATATTTTTCAGAAAAATGAGACCCAAGATTACGCTTAAGATTTTCGCTCAGCTATTTGTTCTGGCTCTTCTTGGGTTAGTATTAAAGCAGGTTGGTCCAAACAAACTAACACGTTCCTTTGAATTATCTAAAAACCTTTTCTTTTTGTGTCCTTTTTTGTATCTTCCATGGTATCGATCTCAACAGGCCACTGATTGATCAGAACTTCTACTACTTGGGGATAAAAATGACATCCCCAACTTTCGCATGTGCTATAAGCAACACGCTTCCTTCAATGACATTCATCATGGCGCTTCTTTGTAGGTATGTTAAGTTTTCGTTAATGTCCTTTCAAATCGAATGAACAGAAAGTACGAAAAATATGAACAAAATGAATAAATATACAACAATAACATAATAACATGGAATTATTGTATTCAAAGTTTAGTTATTTTTTAACTAAAACGGTCATTATCAGAATGGAGAAGTTGGAGTTGAAGAGTGTGAGATGCCAAGCCAAGGTGTTTGGAACAATAGTGACGATGGTGGGAGCCATGTTGATGACATTTTATAAAGGAAGTGTCATCAACTTCTTCTGGACTGGGCATGGCCGCCACCCAAACACACCACCCACGGCGGATGCAGCGGCGGCGTCCAACCTTCACAACGACGGAAAGGTCATCAAAGGCTCCATCTTCCTCATCATCGCCACTCTGGCTTGGGCGGCCTTCTTCATTCTGCAAGTCATAACCATGAGAAAATACACAGCCCATCTTTCTCTCACCACCATGGTTTGCTTTTTAGGAACCCTTCAAGCCATTGTTGTCACCTTCGCCATGGAGCACCGCCCTCACGCCTGGGCCATTGGCTGGGACATGAACCTCCTGGCCGCCGCTTATGCGGTAAGAAAGAAAATAATTGTAACAGTTCAAGCCCTCCATCAGTAGATATTGTCATTTTTATATTTTTCCTTTCGGACTTCCCCTCAAAGTTTTTAAAACGCATCTGCTAAGAAAAGATTTTCATACCCTTATAAACAATGTTTCGCTCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCCTTCGGGGCTCAGTGTCCTCATTGGCACACTGCCTGGTGTCCATCCCTCTTCGGGATTCAGCGTTCTCGATGGCACACCGTCCGATGTCTGGCTCTAATACCAACGGTAACAACCATAGATTTCTACTAACTTAAAGTCGCTACTATAACAGTCCATTTGTAACCGTTCAAGCACACATCACTAGCCGATATTGTCCTTTTTGGGCGTTTTCTTTCAAGCTTCCCTTCAAAGTTTATAAAAAGCATCTATTAGGGAGAGGTTTCCACACCTTATAAACATGTTTCGCTCCCCTCTCCAACCGACATGGGCTCTCACAATAATTGTCCCAGATATTTCCGTCTTTGGATTCTAAAAAACATTCTTCTAATTCTGATTCATAATATTGAATGTTTATGCAGGGGATTGTTTGCTCAGGGTTGGCATATTATCTTCAAGGTTTGGTCATGAAAACAAAAGGGCCAGTTTTTGTTACAGCTTTTTGCCCTCTGGGGACGGTGATTGTTGCCTTCATGGGCTCTCTCATCATGGCTGAGAAGATTTATGTTGGAGGGTAACTCATCAATTCTCTTTTGGAGGGTAACCAATCTAGCAGAGTCCTTTAGAACACAACACTCTTATTTTGTACTGATTTTTCAGGATAATTGGAGCAGTGCTGATTGCGGTAGGGCTGTACTGTGTCTTATGGGGTAAATGCAAGGGTGTTGAAGACACTGCTGAAGCAGTGAAAGGTGAAGAATTGCCTGTAAAAAACGAAGCCATTGAAGCAACTCAAAAGAAAGAAGGTTTGGCCCTTAGTATTCCACCCAGCATGGAGAAGAAGCTTCAACACACTAATCACCCAAACTAAGAATAAAAAAGAGAGAATCCTGAAGAACAATAACAGAGAAATGGCCAAGAAGACAATTGACCTTATAATTTACAAATCAATTTGAATATAGCTCGATAATTAAAAATAGCAATTACTATTTCAAAAGCCGATGGAAGATTCCACTAAGCTGGTTGTCTTTTGAGTGGCAGATTCCTAATTGTCACTAAACAGCATAATAATCTCTTTTGTTCTTTAATGTTAATAATATAACCAACTCCAACTCTGTGTTTTTTAGGATTAAGATCACTTAGGTCACCACCACCTCCCATCATTCTTCCTGTGGCCCGTTTTTCGTATCAAT

mRNA sequence

CTTCTTCTCCATACCACTCTTTCCCTTTTATACATAACAAAACAAGCATGGGATACTTGCAATCCCTTGGCTGTCTCCTTCAAAATGCAAAGCCTTACATCGTTGTCATCTCCCTCCAATTTGGCTATGGCGGAATGAATATCATCTCTGCCATCTCCCTCAACCACGGTATGAGCCACTACGTTCTCGTTGTCTACCGCCACGCCTTTGCCACAGTCGTCATGGCACCTTTTGCTCTTGTTTTTGAGAGAAAAATGAGACCCAAGATTACGCTTAAGATTTTCGCTCAGCTATTTGTTCTGGCTCTTCTTGGGCCACTGATTGATCAGAACTTCTACTACTTGGGGATAAAAATGACATCCCCAACTTTCGCATGTGCTATAAGCAACACGCTTCCTTCAATGACATTCATCATGGCGCTTCTTTGTAGAATGGAGAAGTTGGAGTTGAAGAGTGTGAGATGCCAAGCCAAGGTGTTTGGAACAATAGTGACGATGGTGGGAGCCATGTTGATGACATTTTATAAAGGAAGTGTCATCAACTTCTTCTGGACTGGGCATGGCCGCCACCCAAACACACCACCCACGGCGGATGCAGCGGCGGCGTCCAACCTTCACAACGACGGAAAGGTCATCAAAGGCTCCATCTTCCTCATCATCGCCACTCTGGCTTGGGCGGCCTTCTTCATTCTGCAAGTCATAACCATGAGAAAATACACAGCCCATCTTTCTCTCACCACCATGGTTTGCTTTTTAGGAACCCTTCAAGCCATTGTTGTCACCTTCGCCATGGAGCACCGCCCTCACGCCTGGGCCATTGGCTGGGACATGAACCTCCTGGCCGCCGCTTATGCGGGGATTGTTTGCTCAGGGTTGGCATATTATCTTCAAGGTTTGGTCATGAAAACAAAAGGGCCAGTTTTTGTTACAGCTTTTTGCCCTCTGGGGACGGTGATTGTTGCCTTCATGGGCTCTCTCATCATGGCTGAGAAGATTTATGTTGGAGGGATAATTGGAGCAGTGCTGATTGCGGTAGGGCTGTACTGTGTCTTATGGGGTAAATGCAAGGGTGTTGAAGACACTGCTGAAGCAGTGAAAGGTGAAGAATTGCCTGTAAAAAACGAAGCCATTGAAGCAACTCAAAAGAAAGAAGGTTTGGCCCTTAGTATTCCACCCAGCATGGAGAAGAAGCTTCAACACACTAATCACCCAAACTAAGAATAAAAAAGAGAGAATCCTGAAGAACAATAACAGAGAAATGGCCAAGAAGACAATTGACCTTATAATTTACAAATCAATTTGAATATAGCTCGATAATTAAAAATAGCAATTACTATTTCAAAAGCCGATGGAAGATTCCACTAAGCTGGTTGTCTTTTGAGTGGCAGATTCCTAATTGTCACTAAACAGCATAATAATCTCTTTTGTTCTTTAATGTTAATAATATAACCAACTCCAACTCTGTGTTTTTTAGGATTAAGATCACTTAGGTCACCACCACCTCCCATCATTCTTCCTGTGGCCCGTTTTTCGTATCAAT

Coding sequence (CDS)

ATGGGATACTTGCAATCCCTTGGCTGTCTCCTTCAAAATGCAAAGCCTTACATCGTTGTCATCTCCCTCCAATTTGGCTATGGCGGAATGAATATCATCTCTGCCATCTCCCTCAACCACGGTATGAGCCACTACGTTCTCGTTGTCTACCGCCACGCCTTTGCCACAGTCGTCATGGCACCTTTTGCTCTTGTTTTTGAGAGAAAAATGAGACCCAAGATTACGCTTAAGATTTTCGCTCAGCTATTTGTTCTGGCTCTTCTTGGGCCACTGATTGATCAGAACTTCTACTACTTGGGGATAAAAATGACATCCCCAACTTTCGCATGTGCTATAAGCAACACGCTTCCTTCAATGACATTCATCATGGCGCTTCTTTGTAGAATGGAGAAGTTGGAGTTGAAGAGTGTGAGATGCCAAGCCAAGGTGTTTGGAACAATAGTGACGATGGTGGGAGCCATGTTGATGACATTTTATAAAGGAAGTGTCATCAACTTCTTCTGGACTGGGCATGGCCGCCACCCAAACACACCACCCACGGCGGATGCAGCGGCGGCGTCCAACCTTCACAACGACGGAAAGGTCATCAAAGGCTCCATCTTCCTCATCATCGCCACTCTGGCTTGGGCGGCCTTCTTCATTCTGCAAGTCATAACCATGAGAAAATACACAGCCCATCTTTCTCTCACCACCATGGTTTGCTTTTTAGGAACCCTTCAAGCCATTGTTGTCACCTTCGCCATGGAGCACCGCCCTCACGCCTGGGCCATTGGCTGGGACATGAACCTCCTGGCCGCCGCTTATGCGGGGATTGTTTGCTCAGGGTTGGCATATTATCTTCAAGGTTTGGTCATGAAAACAAAAGGGCCAGTTTTTGTTACAGCTTTTTGCCCTCTGGGGACGGTGATTGTTGCCTTCATGGGCTCTCTCATCATGGCTGAGAAGATTTATGTTGGAGGGATAATTGGAGCAGTGCTGATTGCGGTAGGGCTGTACTGTGTCTTATGGGGTAAATGCAAGGGTGTTGAAGACACTGCTGAAGCAGTGAAAGGTGAAGAATTGCCTGTAAAAAACGAAGCCATTGAAGCAACTCAAAAGAAAGAAGGTTTGGCCCTTAGTATTCCACCCAGCATGGAGAAGAAGCTTCAACACACTAATCACCCAAACTAA

Protein sequence

MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKEGLALSIPPSMEKKLQHTNHPN
Homology
BLAST of CmoCh14G005030 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 5.0e-117
Identity = 221/369 (59.89%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           M  + S    L ++KPY  +ISLQFGY GMNII+ ISLN GMSHYVLVVYRHA AT V+A
Sbjct: 3   MEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIA 62

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFA  FERK +PKIT  IF QLF+L LLGP+IDQNFYY+G+K TSPTF+CA+SN LP+MT
Sbjct: 63  PFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMT 122

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FI+A+L RME L+LK + CQAK+ GT+VT+ GAMLMT YKG ++  FWT +  H      
Sbjct: 123 FILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKY-MHIQDSSH 182

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAH-LSLTTMVCFLGTL 240
           A+  ++ N  +D + +KGSI LI ATLAWA+ F+LQ   ++ Y  H LSLTT++CF+GTL
Sbjct: 183 ANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTL 242

Query: 241 QAIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPL 300
           QA+ VTF MEH P AW IGWDMNLLAAAY+GIV S ++YY+QG+VMK +GPVF TAF PL
Sbjct: 243 QAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPL 302

Query: 301 GTVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDT-AEAVKGEELPVKN 360
             VIVA MGS ++AEKI++GG+IGAVLI +GLY VLWGK K  + T  E  K +      
Sbjct: 303 MMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVT 362

Query: 361 EAIEATQKK 368
           E +EA   K
Sbjct: 363 EDVEANGSK 370

BLAST of CmoCh14G005030 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.9e-102
Identity = 192/361 (53.19%), Postives = 267/361 (73.96%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGY GMN+++   L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRMEK 131
            K+T  IF ++F+LALLGP+IDQN YY+G+K+TSPTF+ A+SN +P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLHN 191
           +E++ VRC  KV GT+VT+VG++LM FYKG  INFF   H    ++PPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 DGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEHR 251
               +K ++FL++A+L+WA+FF+LQ  T++KY+AHLS++TMVCF+GTLQ++ + F MEH 
Sbjct: 187 ---YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHN 246

Query: 252 PHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGSLI 311
           P A  IG+DMNLLA+AYAGI+ S +AYY+QGL+M+ KGPVFVTAF PL  VIV+ M   +
Sbjct: 247 PSALNIGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFV 306

Query: 312 MAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKEGLA 371
           + + IY+GG+IG V++ VG+Y VLWG  K V+D  E  + E+  V   A++      GL 
Sbjct: 307 LGQGIYLGGVIGVVVLMVGVYAVLWG--KHVDDDGEETRHEDNVV---AVKCCSGNNGLT 349

Query: 372 L 373
           +
Sbjct: 367 I 349

BLAST of CmoCh14G005030 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 8.6e-93
Identity = 179/371 (48.25%), Postives = 266/371 (71.70%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGY GMN+++ + L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRMEK 131
           PK+T  IF Q+FVLALLGPLIDQN YY G+K+TSPTFA A++N +P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLHN 191
           +E++ VR QAKV GT+V +VGAMLM  +K  +I F  +    H  +P   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 DGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEHR 251
               +K ++FL+IA+ +WA+FF+LQ  T+++Y++HLSL+TMVCF+GTLQ+  +TF ME  
Sbjct: 187 ---YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPN 246

Query: 252 PHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGSLI 311
             AW IG+DMNLLA+AYAGI+ S +AYY+QG++ K K  +FVTAF PL  +I + +G LI
Sbjct: 247 LSAWNIGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLI 306

Query: 312 MAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKEGLA 371
           + + + +GG++G  ++ VG+  VLWGK +G  D  E ++ + +    E ++   + +   
Sbjct: 307 LNQTLNLGGVLGMAILVVGVCTVLWGK-EGDIDEEENIEEKFV----EIVKCCNRCDIKV 360

Query: 372 LSIPPSMEKKL 383
           LS+ P +++++
Sbjct: 367 LSMMPRIDEEV 360

BLAST of CmoCh14G005030 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.9e-88
Identity = 170/366 (46.45%), Postives = 245/366 (66.94%), Query Frame = 0

Query: 10  LLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERK 69
           L+ + KPY+ +IS+QFGY GM II+ +SL HGM+HYVL VYRHA AT V+APFAL  ERK
Sbjct: 5   LMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERK 64

Query: 70  MRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRM 129
           +RPK+T +IF Q+ +L  + P++DQN YY+G+  TS TFA A +N LP++TF++A++ R+
Sbjct: 65  IRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRL 124

Query: 130 EKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADA-AAASN 189
           E +  K VR  AKV GT++T+ GA+LMT YKG +++F   G G    +     +   A  
Sbjct: 125 ESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGA 184

Query: 190 LHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAM 249
              D   I G++ L+  T  WA FFILQ  T+++Y A LSLTT++C +GTL+   V+   
Sbjct: 185 AAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVT 244

Query: 250 EHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMG 309
                AW IG+D NL AAAY+G++CSG+AYY+QG+VM+ +GPVFV  F PL  VI A +G
Sbjct: 245 VRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALG 304

Query: 310 SLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKE 369
            ++++E I++G +IG + I VGLY V+WGK K    T +    + LP+K+  ++     +
Sbjct: 305 VVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDEDCKGLPIKS-PVKPVDTGK 364

Query: 370 GLALSI 375
           GLA  +
Sbjct: 365 GLAAEL 369

BLAST of CmoCh14G005030 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.1e-87
Identity = 169/331 (51.06%), Postives = 234/331 (70.69%), Query Frame = 0

Query: 11  LQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKM 70
           ++ A+P+I ++ LQ G  GM+I+S   LN GMS+YVLVVYRHA AT+VMAPFA  F++K+
Sbjct: 10  MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKV 69

Query: 71  RPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRME 130
           RPK+TL IF ++ +L LL P+IDQN YYLG+K T+ TFA A+ N LP++TF++A +  +E
Sbjct: 70  RPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLE 129

Query: 131 KLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLH 190
           +++L+ +R   KV GT+ T+ GAM+MT  KG V++ FWT      NT       A +++H
Sbjct: 130 RVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNT-------AGTDIH 189

Query: 191 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEH 250
           +    IKG++ + I   ++A F ILQ IT+R Y A LSLT  +C +GT++   V   ME 
Sbjct: 190 S---AIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEK 249

Query: 251 -RPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGS 310
             P AWAIGWD  LL A Y+GIVCS LAYY+ G+VMKT+GPVFVTAF PL  +IVA M +
Sbjct: 250 GNPSAWAIGWDTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMST 309

Query: 311 LIMAEKIYVGGIIGAVLIAVGLYCVLWGKCK 341
           +I AE++Y+G ++GAV+I  GLY V+WGK K
Sbjct: 310 IIFAEQMYLGRVLGAVVICAGLYLVIWGKGK 330

BLAST of CmoCh14G005030 vs. ExPASy TrEMBL
Match: A0A6J1F8T0 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111441872 PE=3 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.1e-215
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA
Sbjct: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT
Sbjct: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240
           ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ
Sbjct: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240

Query: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300
           AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG
Sbjct: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300

Query: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEA 360
           TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEA
Sbjct: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEA 360

Query: 361 IEATQKKEGLALSIPPSMEKKLQHTNHPN 390
           IEATQKKEGLALSIPPSMEKKLQHTNHPN
Sbjct: 361 IEATQKKEGLALSIPPSMEKKLQHTNHPN 389

BLAST of CmoCh14G005030 vs. ExPASy TrEMBL
Match: A0A6J1J8I7 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482309 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.1e-202
Identity = 369/389 (94.86%), Postives = 376/389 (96.66%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGYLQSLGCLLQ+AKPYIVVISLQFGY GMNIISAISL+ GMSHYVLVVYRHAFATV+MA
Sbjct: 1   MGYLQSLGCLLQSAKPYIVVISLQFGYAGMNIISAISLDRGMSHYVLVVYRHAFATVIMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFALVFERKM PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT
Sbjct: 61  PFALVFERKMIPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTP T
Sbjct: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPLT 180

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240
           ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ
Sbjct: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240

Query: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300
           AI+VT AMEHRPHAWAIGWDMNLLAAAYAGIV SGLAYYLQGLVMKTKGPVFVTAFCPLG
Sbjct: 241 AIIVTLAMEHRPHAWAIGWDMNLLAAAYAGIVTSGLAYYLQGLVMKTKGPVFVTAFCPLG 300

Query: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEA 360
           T IVAFMGSLI+AEKIYVGGIIGAVLIA GLYCVLWGKCK VED+AEAVKGEELPVKNEA
Sbjct: 301 TAIVAFMGSLILAEKIYVGGIIGAVLIATGLYCVLWGKCKDVEDSAEAVKGEELPVKNEA 360

Query: 361 IEATQKKEGLALSIPPSMEKKLQHTNHPN 390
           IEATQKKE L LSI P+MEKKLQ TNHPN
Sbjct: 361 IEATQKKEALVLSIRPNMEKKLQQTNHPN 389

BLAST of CmoCh14G005030 vs. ExPASy TrEMBL
Match: A0A6J1F9X7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111442149 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 5.1e-165
Identity = 302/361 (83.66%), Postives = 321/361 (88.92%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MGY QS GC  QNA PYIVVISL FGY GMNIIS ISL+ GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGYSQSFGCFFQNAMPYIVVISLTFGYAGMNIISTISLDRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFAL FERK+RPKIT KIF QLFVLALLGP+IDQN YYLG+KMTSPTF+CAI N LPSMT
Sbjct: 61  PFALFFERKVRPKITFKIFVQLFVLALLGPVIDQNLYYLGLKMTSPTFSCAIGNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+LK+VRCQAKV GTIVT+VGAMLMTFYKGSVINFFWTGH RHPNTPPT
Sbjct: 121 FIMAVLCRMEKLDLKNVRCQAKVLGTIVTVVGAMLMTFYKGSVINFFWTGHVRHPNTPPT 180

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240
           ADAAAASN HNDGK IKGSI LIIATLAWAAFFILQ IT+RKYTAHLSLTTMVCFLGTLQ
Sbjct: 181 ADAAAASNPHNDGKFIKGSILLIIATLAWAAFFILQAITLRKYTAHLSLTTMVCFLGTLQ 240

Query: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300
           AIVVT AM+H  H+W+IGWDMNLLAAAY GIV SGLAYYLQGLVMK KGPVFVTAF PL 
Sbjct: 241 AIVVTLAMDHPRHSWSIGWDMNLLAAAYGGIVSSGLAYYLQGLVMKRKGPVFVTAFGPLV 300

Query: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEA 360
            VIV FMGSLI+ E++YVGGIIGAVLI VGLY VLWGKCK VED  EAV+GEELPV N+ 
Sbjct: 301 IVIVGFMGSLILGERVYVGGIIGAVLIGVGLYSVLWGKCKDVEDATEAVEGEELPVINQP 360

Query: 361 I 362
           +
Sbjct: 361 V 361

BLAST of CmoCh14G005030 vs. ExPASy TrEMBL
Match: A0A6J1EZ96 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111440914 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.2e-159
Identity = 304/393 (77.35%), Postives = 334/393 (84.99%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MG+++ LG   QNA+PYI VISLQFGY GMNIIS +SLN GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFALV ERK+RPKIT KIF Q+FVLALLGPLIDQNFYY+G+KMTSPT++CAISN LPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+L+ VRCQAK+FGTIVT++GAMLMTFYKGSVINFFWT    HPN+PPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240
           AD AA  N H+DG+ IKGSI LIIATLAWA FFILQVIT+RKYTAHLSLTTMVCFLGTLQ
Sbjct: 181 ADTAAL-NHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQ 240

Query: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300
           AIVVT AMEHRP AWAIGWDMNLLAAAYAG+V SGLAYY+QGLVMKT+GPVFVTAF PL 
Sbjct: 241 AIVVTLAMEHRPSAWAIGWDMNLLAAAYAGVVASGLAYYVQGLVMKTRGPVFVTAFSPLL 300

Query: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKG------VEDTAEAVKGEEL 360
            VIVAFMGSLI+AEKIYVGGIIGAV+I VGLY VLWGK K        E   EAVKG EL
Sbjct: 301 MVIVAFMGSLILAEKIYVGGIIGAVVIVVGLYSVLWGKYKEGKEKEVGEGVEEAVKGGEL 360

Query: 361 PVKNEAIEATQKKEGLALSIP-PSMEKKLQHTN 387
           PV NE I   +  EGL +S+P P M +KLQ  N
Sbjct: 361 PVTNEGI-VQKNYEGLGISMPAPVMVEKLQAAN 391

BLAST of CmoCh14G005030 vs. ExPASy TrEMBL
Match: A0A6J1I534 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469993 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.7e-158
Identity = 303/393 (77.10%), Postives = 335/393 (85.24%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           MG+++ LG   QNA+PYI VISLQFGY GMNIIS +SLN GMSHYVLVVYRHAFAT VMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFALV ERK+RPKIT KIF Q+FVLALLGPLIDQNFYY+G+KMTSPT++CAISN LPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FIMA+LCRMEKL+L+ VRCQAK+FGTIVT++GAMLMTFYKGSVINFFWT   RHPN+PPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQARHPNSPPT 180

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQ 240
           ADA A  N H+D + IKGSI LIIATLAWA FFILQVIT+RKYTAHLSLTTMVCFLGTLQ
Sbjct: 181 ADAGAL-NHHHDREFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQ 240

Query: 241 AIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLG 300
           AIVVT AMEHRP AWAIGWDMNLLAAAYAG+V SGLAYY+QGLVMKT+GPVFVTAF PL 
Sbjct: 241 AIVVTLAMEHRPSAWAIGWDMNLLAAAYAGVVASGLAYYVQGLVMKTRGPVFVTAFSPLL 300

Query: 301 TVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKG------VEDTAEAVKGEEL 360
            VIVAFMGSLI+AEKIYVGGIIGAV+I +GLY VLWGK +        E   EAVKG EL
Sbjct: 301 MVIVAFMGSLILAEKIYVGGIIGAVVIVMGLYSVLWGKYEEGKEKEVGEGVEEAVKGGEL 360

Query: 361 PVKNEAIEATQKKEGLALSIP-PSMEKKLQHTN 387
           PV NE I   +  EGLA+S+P P M +KLQ  N
Sbjct: 361 PVINEGI-VQKNYEGLAISMPAPVMVEKLQAAN 391

BLAST of CmoCh14G005030 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 422.5 bits (1085), Expect = 3.6e-118
Identity = 221/369 (59.89%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MGYLQSLGCLLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMA 60
           M  + S    L ++KPY  +ISLQFGY GMNII+ ISLN GMSHYVLVVYRHA AT V+A
Sbjct: 3   MEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIA 62

Query: 61  PFALVFERKMRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMT 120
           PFA  FERK +PKIT  IF QLF+L LLGP+IDQNFYY+G+K TSPTF+CA+SN LP+MT
Sbjct: 63  PFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMT 122

Query: 121 FIMALLCRMEKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPT 180
           FI+A+L RME L+LK + CQAK+ GT+VT+ GAMLMT YKG ++  FWT +  H      
Sbjct: 123 FILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKY-MHIQDSSH 182

Query: 181 ADAAAASNLHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAH-LSLTTMVCFLGTL 240
           A+  ++ N  +D + +KGSI LI ATLAWA+ F+LQ   ++ Y  H LSLTT++CF+GTL
Sbjct: 183 ANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTL 242

Query: 241 QAIVVTFAMEHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPL 300
           QA+ VTF MEH P AW IGWDMNLLAAAY+GIV S ++YY+QG+VMK +GPVF TAF PL
Sbjct: 243 QAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPL 302

Query: 301 GTVIVAFMGSLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDT-AEAVKGEELPVKN 360
             VIVA MGS ++AEKI++GG+IGAVLI +GLY VLWGK K  + T  E  K +      
Sbjct: 303 MMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVT 362

Query: 361 EAIEATQKK 368
           E +EA   K
Sbjct: 363 EDVEANGSK 370

BLAST of CmoCh14G005030 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 372.5 bits (955), Expect = 4.2e-103
Identity = 192/361 (53.19%), Postives = 267/361 (73.96%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGY GMN+++   L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRMEK 131
            K+T  IF ++F+LALLGP+IDQN YY+G+K+TSPTF+ A+SN +P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLHN 191
           +E++ VRC  KV GT+VT+VG++LM FYKG  INFF   H    ++PPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 DGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEHR 251
               +K ++FL++A+L+WA+FF+LQ  T++KY+AHLS++TMVCF+GTLQ++ + F MEH 
Sbjct: 187 ---YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHN 246

Query: 252 PHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGSLI 311
           P A  IG+DMNLLA+AYAGI+ S +AYY+QGL+M+ KGPVFVTAF PL  VIV+ M   +
Sbjct: 247 PSALNIGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFV 306

Query: 312 MAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKEGLA 371
           + + IY+GG+IG V++ VG+Y VLWG  K V+D  E  + E+  V   A++      GL 
Sbjct: 307 LGQGIYLGGVIGVVVLMVGVYAVLWG--KHVDDDGEETRHEDNVV---AVKCCSGNNGLT 349

Query: 372 L 373
           +
Sbjct: 367 I 349

BLAST of CmoCh14G005030 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 342.0 bits (876), Expect = 6.1e-94
Identity = 179/371 (48.25%), Postives = 266/371 (71.70%), Query Frame = 0

Query: 12  QNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKMR 71
           ++AKPY  ++ LQFGY GMN+++ + L+ GMSHYVLV YR+AFAT  +APFAL+ ERK+R
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRMEK 131
           PK+T  IF Q+FVLALLGPLIDQN YY G+K+TSPTFA A++N +P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLHN 191
           +E++ VR QAKV GT+V +VGAMLM  +K  +I F  +    H  +P   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 DGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEHR 251
               +K ++FL+IA+ +WA+FF+LQ  T+++Y++HLSL+TMVCF+GTLQ+  +TF ME  
Sbjct: 187 ---YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPN 246

Query: 252 PHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGSLI 311
             AW IG+DMNLLA+AYAGI+ S +AYY+QG++ K K  +FVTAF PL  +I + +G LI
Sbjct: 247 LSAWNIGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLI 306

Query: 312 MAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKEGLA 371
           + + + +GG++G  ++ VG+  VLWGK +G  D  E ++ + +    E ++   + +   
Sbjct: 307 LNQTLNLGGVLGMAILVVGVCTVLWGK-EGDIDEEENIEEKFV----EIVKCCNRCDIKV 360

Query: 372 LSIPPSMEKKL 383
           LS+ P +++++
Sbjct: 367 LSMMPRIDEEV 360

BLAST of CmoCh14G005030 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 327.0 bits (837), Expect = 2.0e-89
Identity = 170/366 (46.45%), Postives = 245/366 (66.94%), Query Frame = 0

Query: 10  LLQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERK 69
           L+ + KPY+ +IS+QFGY GM II+ +SL HGM+HYVL VYRHA AT V+APFAL  ERK
Sbjct: 5   LMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERK 64

Query: 70  MRPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRM 129
           +RPK+T +IF Q+ +L  + P++DQN YY+G+  TS TFA A +N LP++TF++A++ R+
Sbjct: 65  IRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRL 124

Query: 130 EKLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADA-AAASN 189
           E +  K VR  AKV GT++T+ GA+LMT YKG +++F   G G    +     +   A  
Sbjct: 125 ESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGA 184

Query: 190 LHNDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAM 249
              D   I G++ L+  T  WA FFILQ  T+++Y A LSLTT++C +GTL+   V+   
Sbjct: 185 AAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVT 244

Query: 250 EHRPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMG 309
                AW IG+D NL AAAY+G++CSG+AYY+QG+VM+ +GPVFV  F PL  VI A +G
Sbjct: 245 VRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALG 304

Query: 310 SLIMAEKIYVGGIIGAVLIAVGLYCVLWGKCKGVEDTAEAVKGEELPVKNEAIEATQKKE 369
            ++++E I++G +IG + I VGLY V+WGK K    T +    + LP+K+  ++     +
Sbjct: 305 VVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDEDCKGLPIKS-PVKPVDTGK 364

Query: 370 GLALSI 375
           GLA  +
Sbjct: 365 GLAAEL 369

BLAST of CmoCh14G005030 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.9e-88
Identity = 169/331 (51.06%), Postives = 234/331 (70.69%), Query Frame = 0

Query: 11  LQNAKPYIVVISLQFGYGGMNIISAISLNHGMSHYVLVVYRHAFATVVMAPFALVFERKM 70
           ++ A+P+I ++ LQ G  GM+I+S   LN GMS+YVLVVYRHA AT+VMAPFA  F++K+
Sbjct: 10  MEKARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKV 69

Query: 71  RPKITLKIFAQLFVLALLGPLIDQNFYYLGIKMTSPTFACAISNTLPSMTFIMALLCRME 130
           RPK+TL IF ++ +L LL P+IDQN YYLG+K T+ TFA A+ N LP++TF++A +  +E
Sbjct: 70  RPKMTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLE 129

Query: 131 KLELKSVRCQAKVFGTIVTMVGAMLMTFYKGSVINFFWTGHGRHPNTPPTADAAAASNLH 190
           +++L+ +R   KV GT+ T+ GAM+MT  KG V++ FWT      NT       A +++H
Sbjct: 130 RVKLRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNT-------AGTDIH 189

Query: 191 NDGKVIKGSIFLIIATLAWAAFFILQVITMRKYTAHLSLTTMVCFLGTLQAIVVTFAMEH 250
           +    IKG++ + I   ++A F ILQ IT+R Y A LSLT  +C +GT++   V   ME 
Sbjct: 190 S---AIKGAVLVTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEK 249

Query: 251 -RPHAWAIGWDMNLLAAAYAGIVCSGLAYYLQGLVMKTKGPVFVTAFCPLGTVIVAFMGS 310
             P AWAIGWD  LL A Y+GIVCS LAYY+ G+VMKT+GPVFVTAF PL  +IVA M +
Sbjct: 250 GNPSAWAIGWDTKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMST 309

Query: 311 LIMAEKIYVGGIIGAVLIAVGLYCVLWGKCK 341
           +I AE++Y+G ++GAV+I  GLY V+WGK K
Sbjct: 310 IIFAEQMYLGRVLGAVVICAGLYLVIWGKGK 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FL415.0e-11759.89WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
F4IJ085.9e-10253.19WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2... [more]
Q9LXX88.6e-9348.25WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2... [more]
F4HZQ72.9e-8846.45WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Q9ZUS14.1e-8751.06WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1F8T01.1e-215100.00WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111441872 PE=3 SV=1[more]
A0A6J1J8I71.1e-20294.86WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111482309 PE=3 SV=1[more]
A0A6J1F9X75.1e-16583.66WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111442149 PE=3 SV=1[more]
A0A6J1EZ963.2e-15977.35WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111440914 PE=3 SV=1[more]
A0A6J1I5342.7e-15877.10WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469993 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07050.13.6e-11859.89nodulin MtN21 /EamA-like transporter family protein [more]
AT2G40900.14.2e-10353.19nodulin MtN21 /EamA-like transporter family protein [more]
AT3G56620.16.1e-9448.25nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.12.0e-8946.45nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37460.12.9e-8851.06nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 16..157
e-value: 1.3E-12
score: 48.1
coord: 197..335
e-value: 8.2E-12
score: 45.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 367..389
NoneNo IPR availablePANTHERPTHR31218:SF342WAT1-RELATED PROTEINcoord: 11..356
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 50..159
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 236..338
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 11..356

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh14G005030.1CmoCh14G005030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity