CmoCh13G009540 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G009540
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NRT1/ PTR FAMILY 2.8
LocationCmo_Chr13: 8296425 .. 8298326 (-)
RNA-Seq ExpressionCmoCh13G009540
SyntenyCmoCh13G009540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTTGGAATCCCCACTTCCCTCCTCTCCTTCAAACTCGCACCACCGCCATCCGCCCCCTCGCCGGCAGCCAGGAGGGTGGCGCGCCGTCAAATACATCATCGGTAACACAAAACACTTCAACAACACAACAAGCTCCTCTGTATTTTTCCTTTGGTTTTTAATGCATCTTCTTCTTCTTCTTCTTAAGGGAACGAGTCGTTTGAGAAGCTGTCATCCATGAGTCTGATCTCCAATATCACTGTATATCTCAGCACCAAATACAATCTCAACGGCATTTATGTGGTCAATGTGGTTAACATTTGGAGTGGCACCTCCAATATTGCCACTTTGGCCGGCGCTTTCATCGCCGATACTTGCCTTGGCCGCTACCGCACCCTCCTCTACGGCTCCATTGCATCATTATTGGTATTATCAACATACCCTTTTGAATTCATCAACATTTATGCAATTCTTTTGATTTCAAATTCGTTTTCATTAGGGAATGGGGGCTGTTACGCTCACTGCAATCTTCCGCCAGCTCAGACCTTCTTCTTGCAATGCTCAAAATCCCGACCATTGCCCACAGCCACATTTATGGCAGCTCCTCGTTCTCTTCACTGGTCTCGGTCTGTTATCCATCGGCGCCGGTGGCATTCGTCCCTGCAACGTCGCATTCGGAGCCGATCAATTCGACACCACCACAGAAAAGGGAAAGTCCCAATTAGAAAGCTTCTTTAATTGGTGGTATCTCTCTTTCACCGTTGCTCTGCTTATAGCCCTCACCGGTGTTGTTTATGTTCAAACAAATATAAGTTGGACCCTCGGATTCGCCATACCCACCATGTGTTTCTTCTTCTCCATCACGATTTTCTTGTTGGGTCGCCATACTTACATCATGGCCGAGCCCAGAGGAAGCATGTTTTCTGATATGGCTCGTGTCATTATCTCTGCTTGTAGAAAACGAAGATACTCTGTTTCGTCTTACTCGTTTTATGACCCATCAATGGCGGATTCTTCCCATGAGGAAAAGCTTGTTCATACAGAGAGATTCAAATGGCTGGATAAAGCTGCGATTATAGTGAACCCAGATGAAGAATTGGATGAACAGGGGAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAGGTGGAAGGATTCAAATGCCTTGTGTCCATTATTCCCATTTGGATATCAGGAATTGGGTGTTTTGTAGTGTTCAATCAACCAAACACATTTGGAATTCTTCAAGCTTTGCAATCAAACAGATCAATCGGAACCCATTTCAAATTCCCACCCGGCTGGATGCATCTGGCCGGAATGATTTCTCTATCAATATGGATCATAATCTACGAGAGGGTTTTCATCAAAATGGCAAAGAAAATCACCGGAAAAGAGAGAAGACTTACAATGAAACAGAGGATCACGATAGGGATAGTGGTGTCGATTGTGTGCATGGTCGTCTCCGGCATTGTCGAGAGATATCGACGGGAGGCTGCTTTGAGAAACGGGTCATTCATTTCACCGATAAGCTTCGCATTCCTGTTGCCGCAGCACGCTCTTACTGGTTTAATGGAGGCTTTTGCGTTGGTAGCCATTATGGAGTTCTTCACGATGCATATGCCAGAGCATATGAGAACGGTTGCAGGGGCCATCTTCTTCCTCACGCTCTCTGTTGCGAGCTATTTGAGCTCTTTAATAGTTAATCTGATACAGACTGTGAGCGGTGAATTTGCAGAATCGGCATGGGTAGGGGGTCATGACTTGAACGAGAATAGGCTCGATTACTACTATTTCACGATCGCCATTGTCGGAGCTCTGAATCTGCTGTACTTCGTGTTGTTTGCAAGCCGTTTTGTGACGAGTTATGATAATAAAGTGAAATTGATGGAAGATTTGAATCGGATTGATTTGTAA

mRNA sequence

ATGGACTTGGAATCCCCACTTCCCTCCTCTCCTTCAAACTCGCACCACCGCCATCCGCCCCCTCGCCGGCAGCCAGGAGGGTGGCGCGCCGTCAAATACATCATCGGGAACGAGTCGTTTGAGAAGCTGTCATCCATGAGTCTGATCTCCAATATCACTGTATATCTCAGCACCAAATACAATCTCAACGGCATTTATGTGGTCAATGTGGTTAACATTTGGAGTGGCACCTCCAATATTGCCACTTTGGCCGGCGCTTTCATCGCCGATACTTGCCTTGGCCGCTACCGCACCCTCCTCTACGGCTCCATTGCATCATTATTGGGAATGGGGGCTGTTACGCTCACTGCAATCTTCCGCCAGCTCAGACCTTCTTCTTGCAATGCTCAAAATCCCGACCATTGCCCACAGCCACATTTATGGCAGCTCCTCGTTCTCTTCACTGGTCTCGGTCTGTTATCCATCGGCGCCGGTGGCATTCGTCCCTGCAACGTCGCATTCGGAGCCGATCAATTCGACACCACCACAGAAAAGGGAAAGTCCCAATTAGAAAGCTTCTTTAATTGGTGGTATCTCTCTTTCACCGTTGCTCTGCTTATAGCCCTCACCGGTGTTGTTTATGTTCAAACAAATATAAGTTGGACCCTCGGATTCGCCATACCCACCATGTGTTTCTTCTTCTCCATCACGATTTTCTTGTTGGGTCGCCATACTTACATCATGGCCGAGCCCAGAGGAAGCATGTTTTCTGATATGGCTCGTGTCATTATCTCTGCTTGTAGAAAACGAAGATACTCTGTTTCGTCTTACTCGTTTTATGACCCATCAATGGCGGATTCTTCCCATGAGGAAAAGCTTGTTCATACAGAGAGATTCAAATGGCTGGATAAAGCTGCGATTATAGTGAACCCAGATGAAGAATTGGATGAACAGGGGAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAGGTGGAAGGATTCAAATGCCTTGTGTCCATTATTCCCATTTGGATATCAGGAATTGGGTGTTTTGTAGTGTTCAATCAACCAAACACATTTGGAATTCTTCAAGCTTTGCAATCAAACAGATCAATCGGAACCCATTTCAAATTCCCACCCGGCTGGATGCATCTGGCCGGAATGATTTCTCTATCAATATGGATCATAATCTACGAGAGGGTTTTCATCAAAATGGCAAAGAAAATCACCGGAAAAGAGAGAAGACTTACAATGAAACAGAGGATCACGATAGGGATAGTGGTGTCGATTGTGTGCATGGTCGTCTCCGGCATTGTCGAGAGATATCGACGGGAGGCTGCTTTGAGAAACGGGTCATTCATTTCACCGATAAGCTTCGCATTCCTGTTGCCGCAGCACGCTCTTACTGGTTTAATGGAGGCTTTTGCGTTGGTAGCCATTATGGAGTTCTTCACGATGCATATGCCAGAGCATATGAGAACGGTTGCAGGGGCCATCTTCTTCCTCACGCTCTCTGTTGCGAGCTATTTGAGCTCTTTAATAGTTAATCTGATACAGACTGTGAGCGGTGAATTTGCAGAATCGGCATGGGTAGGGGGTCATGACTTGAACGAGAATAGGCTCGATTACTACTATTTCACGATCGCCATTGTCGGAGCTCTGAATCTGCTGTACTTCGTGTTGTTTGCAAGCCGTTTTGTGACGAGTTATGATAATAAAGTGAAATTGATGGAAGATTTGAATCGGATTGATTTGTAA

Coding sequence (CDS)

ATGGACTTGGAATCCCCACTTCCCTCCTCTCCTTCAAACTCGCACCACCGCCATCCGCCCCCTCGCCGGCAGCCAGGAGGGTGGCGCGCCGTCAAATACATCATCGGGAACGAGTCGTTTGAGAAGCTGTCATCCATGAGTCTGATCTCCAATATCACTGTATATCTCAGCACCAAATACAATCTCAACGGCATTTATGTGGTCAATGTGGTTAACATTTGGAGTGGCACCTCCAATATTGCCACTTTGGCCGGCGCTTTCATCGCCGATACTTGCCTTGGCCGCTACCGCACCCTCCTCTACGGCTCCATTGCATCATTATTGGGAATGGGGGCTGTTACGCTCACTGCAATCTTCCGCCAGCTCAGACCTTCTTCTTGCAATGCTCAAAATCCCGACCATTGCCCACAGCCACATTTATGGCAGCTCCTCGTTCTCTTCACTGGTCTCGGTCTGTTATCCATCGGCGCCGGTGGCATTCGTCCCTGCAACGTCGCATTCGGAGCCGATCAATTCGACACCACCACAGAAAAGGGAAAGTCCCAATTAGAAAGCTTCTTTAATTGGTGGTATCTCTCTTTCACCGTTGCTCTGCTTATAGCCCTCACCGGTGTTGTTTATGTTCAAACAAATATAAGTTGGACCCTCGGATTCGCCATACCCACCATGTGTTTCTTCTTCTCCATCACGATTTTCTTGTTGGGTCGCCATACTTACATCATGGCCGAGCCCAGAGGAAGCATGTTTTCTGATATGGCTCGTGTCATTATCTCTGCTTGTAGAAAACGAAGATACTCTGTTTCGTCTTACTCGTTTTATGACCCATCAATGGCGGATTCTTCCCATGAGGAAAAGCTTGTTCATACAGAGAGATTCAAATGGCTGGATAAAGCTGCGATTATAGTGAACCCAGATGAAGAATTGGATGAACAGGGGAAACCCAAGAATCCATGGAGGTTATGCAGTTTACAGCAGGTGGAAGGATTCAAATGCCTTGTGTCCATTATTCCCATTTGGATATCAGGAATTGGGTGTTTTGTAGTGTTCAATCAACCAAACACATTTGGAATTCTTCAAGCTTTGCAATCAAACAGATCAATCGGAACCCATTTCAAATTCCCACCCGGCTGGATGCATCTGGCCGGAATGATTTCTCTATCAATATGGATCATAATCTACGAGAGGGTTTTCATCAAAATGGCAAAGAAAATCACCGGAAAAGAGAGAAGACTTACAATGAAACAGAGGATCACGATAGGGATAGTGGTGTCGATTGTGTGCATGGTCGTCTCCGGCATTGTCGAGAGATATCGACGGGAGGCTGCTTTGAGAAACGGGTCATTCATTTCACCGATAAGCTTCGCATTCCTGTTGCCGCAGCACGCTCTTACTGGTTTAATGGAGGCTTTTGCGTTGGTAGCCATTATGGAGTTCTTCACGATGCATATGCCAGAGCATATGAGAACGGTTGCAGGGGCCATCTTCTTCCTCACGCTCTCTGTTGCGAGCTATTTGAGCTCTTTAATAGTTAATCTGATACAGACTGTGAGCGGTGAATTTGCAGAATCGGCATGGGTAGGGGGTCATGACTTGAACGAGAATAGGCTCGATTACTACTATTTCACGATCGCCATTGTCGGAGCTCTGAATCTGCTGTACTTCGTGTTGTTTGCAAGCCGTTTTGTGACGAGTTATGATAATAAAGTGAAATTGATGGAAGATTTGAATCGGATTGATTTGTAA

Protein sequence

MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Homology
BLAST of CmoCh13G009540 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 630.9 bits (1626), Expect = 1.4e-179
Identity = 318/566 (56.18%), Postives = 424/566 (74.91%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MD+ES  PSS       H   +++ GGWRA+KYII NESFEKL+SMSLI N++VYL TKY
Sbjct: 1   MDVESSSPSS-------HALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NL G+++VNV+NIW G+ NI TLAGAF++D  LGR+ TLL GSIAS +GMG   LTA   
Sbjct: 61  NLGGVFLVNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALP 120

Query: 121 QLRPSSCNAQNPDHCP-QPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKG 180
            LRP +C   +P +C  QP  WQL VLF+GLGLL+IGAGG+RPCN+AFGADQFDT+T+KG
Sbjct: 121 SLRPDAC--IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKG 180

Query: 181 KSQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTY 240
           K+ LE+FFNWWY SFTVAL+IALTGVVY+QTNISW +GF IPT C   SIT F++G+HTY
Sbjct: 181 KAHLETFFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTY 240

Query: 241 IMAEPRGSMFSDMARVIISACRKRRYSV-SSYSFYDPSMADSSHEEKLVHTERFKWLDKA 300
           I A+  GS+F+D+ +V+ +AC+KR+    S  +FY     D S    +    R ++ DKA
Sbjct: 241 ICAKAEGSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKA 300

Query: 301 AIIVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGIL 360
           +I+ NP+ EL+E G  K  WRLCS+QQV+  KC+ +I+P+W++GI CF++ +Q N +GIL
Sbjct: 301 SIVTNPN-ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGIL 360

Query: 361 QALQSNRSIGTH-FKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRI 420
           QA+Q +++ G H F+ P GWM+L  MI+L+IWI +YE V I + K+ITG+++RLT+K RI
Sbjct: 361 QAMQMDKTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRI 420

Query: 421 TIGIVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIME 480
              IV+ I+CM+V+G  E+ RR +AL+NGSF+SP+S   LLPQ AL GL EAF+ VA+ME
Sbjct: 421 E--IVMGIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALME 480

Query: 481 FFTMHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLD 540
           F T+ MPEHMR VAGAIFFL+ S+ASY+ +L++N+I  V+ +  +S W+G  DLN+NRL+
Sbjct: 481 FLTVRMPEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKS-WLGDKDLNKNRLE 540

Query: 541 YYYFTIAIVGALNLLYFVLFASRFVT 564
            Y+F IA +   NLLYF LFASR+ T
Sbjct: 541 NYFFIIAGIQVANLLYFRLFASRYAT 553

BLAST of CmoCh13G009540 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 3.8e-129
Identity = 231/539 (42.86%), Postives = 360/539 (66.79%), Query Frame = 0

Query: 23  RQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIAT 82
           ++PGGWRAV +I+GNE+ E+L S+ L++N  VYL+  ++L  +   NV+NIWSG +N+  
Sbjct: 50  KKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTP 109

Query: 83  LAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQ 142
           L GA+I+DT +GR++T+ + S A+LLG+  +TLTA F QL P+SCN+Q+P  C  P+  Q
Sbjct: 110 LVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQ 169

Query: 143 LLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIAL 202
           + VL  GL  LS+G+GGIRPC++ FG DQFD  TE+G   + SFFNW+Y++FTV L+I  
Sbjct: 170 IGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQ 229

Query: 203 TGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRK 262
           T VVY+Q  +SW +GF+IPT     ++ +F  G   Y+  +P GS+FS +A+VI++A +K
Sbjct: 230 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 289

Query: 263 RRYSV-----SSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNP 322
           R+  +      + ++YDP++  SS   KL  + +F+ LDKAA+++  D  L  +G P + 
Sbjct: 290 RKLKLPAEDDGTVTYYDPAI-KSSVLSKLHRSNQFRCLDKAAVVIEGD--LTPEGPPADK 349

Query: 323 WRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPPGW 382
           WRLCS+Q+VE  KCL+ I+PIW +GI          TF + QAL+ +R++G  F+ P G 
Sbjct: 350 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 409

Query: 383 MHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERY 442
           + +  ++++ I++  Y+RVF+   ++ITG +  +T+ QRI  GIV +I  M+V+GIVER 
Sbjct: 410 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 469

Query: 443 RREAALRNG--SFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 502
           RR  ++  G  + ++P+S  +L PQ  L GL EAF ++  +EFF    PEHMR++A ++F
Sbjct: 470 RRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLF 529

Query: 503 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYF 555
            L+ + +SYLSS +V ++   SG      W+   +LN  +LDY+Y+ IA++G +NL+YF
Sbjct: 530 SLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVYF 584

BLAST of CmoCh13G009540 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 427.6 bits (1098), Expect = 2.3e-118
Identity = 226/561 (40.29%), Postives = 349/561 (62.21%), Query Frame = 0

Query: 21  PRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNI 80
           P ++ GGWRA+ +I+GNE+ EKL S+ + +N  +YL   +++  +   NV  +W G +N 
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 81  ATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHL 140
           A L GA I+D  +GR++T+ Y S+ S+LG+  VTLTA   QL P  CN  +PD C  P+ 
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 141 WQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLI 200
            QL +LF GLG LSIG+GGIRPC++ FG DQFD  TE+G   + SFFNW+YL+ T+ L+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 201 ALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISAC 260
           + T VVY+QT +SW +GF+IPT     ++ +F +G   Y+  +P GS+FS +ARVI++A 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 261 RKRRYSVS-----SYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 320
           +KR   +S     +  +Y+P +       KL  T++FK+LDKAA+I+  D +L  +G P 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGV-LSKLPLTDQFKFLDKAAVIL--DGDLTSEGVPA 310

Query: 321 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 380
           N WRLCS+Q+VE  KCL+ ++P+W +GI   V      TF + QA + +R +G HF+ P 
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 381 GWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVE 440
             + +   I++ IW+ IYE + +    ++  ++ R+T+ QR+ IGIV +I+ M  +G VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 441 RYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 500
             RR  A      ++ +S  +L     L GL E+F  + ++EFF    PEHMR++A ++F
Sbjct: 431 GVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 490

Query: 501 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFV 560
            L+ + A+YLSSL+V  +  VSG      W+   DL+  +LDY+Y+ IA++G +NL+YF 
Sbjct: 491 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFW 550

Query: 561 LFASRFVTSYDNKVKLMEDLN 577
             A R+     +++   ED N
Sbjct: 551 YCAHRYQYKAGSQI---EDFN 557

BLAST of CmoCh13G009540 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.2e-114
Identity = 218/540 (40.37%), Postives = 337/540 (62.41%), Query Frame = 0

Query: 27  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIATLAGA 86
           GW+ + +IIGNE+FEKL  +   SN+ +YL+T +N+  I    VVNI+ GTSN  T+  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 87  FIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQLLVL 146
           F+ D+  GRY+TL +  IA  LG  A+ LTA+   L P+ C  +    C  P + Q++ L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 147 FTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIALTGVV 206
              + LL IGAGGIRPCN+ FGADQFD  T++GK  +ESFFNW++ +FT A +++LT +V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 207 YVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRKRRYS 266
           YVQ+N+SW++G AIP +       IF  G   Y+  +  GS    + RVI+ A +KRR  
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 267 VSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNPWRLCSLQQV 326
               +     +A      KL HTE+F++LDK+A I   D++L++ G P + W+LCS+QQV
Sbjct: 262 PVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQV 321

Query: 327 EGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGT-HFKFPPGWMHLAGMIS 386
           E  KC++ ++P+W+S    ++ + Q  T+ I Q+LQS+R +G   F+ P G   +  M+ 
Sbjct: 322 EEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLMLG 381

Query: 387 LSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERYRREAAL-- 446
           ++I+I IY+RV +   +K TG++  +T  QR+  G+ + I  M+VS IVE+YRR+ AL  
Sbjct: 382 MTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALTK 441

Query: 447 -------RNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIFF 506
                  R G+ IS +S  +L+PQ  L G+ +A A V  MEF+    PE+MR+ AG++++
Sbjct: 442 PTLGLAPRKGA-ISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 507 LTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFVL 557
             + +ASYLS+ +++ +   +  F+  +W+   DLN+ RL+Y+YF +A +  LNL YF+L
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWL-PEDLNKGRLEYFYFLVAGMMTLNLAYFLL 558

BLAST of CmoCh13G009540 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.5e-113
Identity = 223/575 (38.78%), Postives = 344/575 (59.83%), Query Frame = 0

Query: 27  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIATLAGA 86
           GW+ + +IIGNE+FEKL  +  +SN+ VYL+  +NL  I    ++N +SGT N  T   A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 87  FIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQLLVL 146
           F+ DT  GRY+TL    IA  LG   + LTA   QL P++C       C  P   Q+  L
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFL 165

Query: 147 FTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIALTGVV 206
             GLG L +GAGGIRPCN+AFGADQF+  +E GK  ++SFFNW++ +FT A +++LT VV
Sbjct: 166 LMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVV 225

Query: 207 YVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRKRRYS 266
           YVQ+N+SWT+G  IP +  F +  IF  G   Y+  +  GS  + +A+VI  A +KR   
Sbjct: 226 YVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLK 285

Query: 267 ------VSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNPWRL 326
                 ++ Y++Y P  A+S    KL +T++F++LDKAAI+  P+++L   GKP +PW+L
Sbjct: 286 PAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKL 345

Query: 327 CSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGT-HFKFPPGWMH 386
           C++QQVE  KC+V ++PIW +    ++   Q  T+ + QALQS+R +G+  F  P     
Sbjct: 346 CTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYV 405

Query: 387 LAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERYRR 446
           +  M  ++++I++Y+RV +   ++ITG +  +T+ QRI  GI  +   +VV+G VE  RR
Sbjct: 406 VFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRR 465

Query: 447 EAAL---------RNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTV 506
             AL         R G  IS +S  +L+PQ +L G+ EAFA +  MEF+    PE+MR+ 
Sbjct: 466 TFALTKPTLGMAPRKGE-ISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSF 525

Query: 507 AGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALN 566
           AG+IF++   V+SYL S ++  +   +   +   W+   DLN+ RLD +YF IA + A+N
Sbjct: 526 AGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYFMIAGILAVN 585

Query: 567 LLYFVLFASRF--------VTSYDNKVKLMEDLNR 578
             YF++ +  +        VT+Y+    +++  ++
Sbjct: 586 FAYFLVMSRWYRYKGSDDEVTTYETNENIIKQQDK 613

BLAST of CmoCh13G009540 vs. ExPASy TrEMBL
Match: A0A6J1E886 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita moschata OX=3662 GN=LOC111431644 PE=3 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR
Sbjct: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI
Sbjct: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of CmoCh13G009540 vs. ExPASy TrEMBL
Match: A0A6J1KHH5 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita maxima OX=3661 GN=LOC111495296 PE=3 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 571/580 (98.45%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHR PPPRRQPGGW AVKYIIGNESFEKLSSMSLISNITVYL+TKY
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWCAVKYIIGNESFEKLSSMSLISNITVYLTTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMGAVTLTAIF 
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPS CNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPT+CFFFSITIFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVII+ACRKRRYSVSSYSFY+P MADSSHEEKLVHTERFKWLDKAAI
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWM+LAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           I++SIVCMVVSGIVERYRREAAL+NGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLI +VSGEFAES WVGGHDLNENRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVG LNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 541 FTIAIVGTLNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of CmoCh13G009540 vs. ExPASy TrEMBL
Match: A0A0A0LQR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045580 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.0e-270
Identity = 468/580 (80.69%), Postives = 519/580 (89.48%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDL +PLPSS     H  PPP ++ GGWRAVKYIIGNESFEKLSSMSLISNITVYLST+Y
Sbjct: 1   MDLVAPLPSS-----HPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTQY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           N+NG +VVNVVNIW GTSNIATLAGAFIADT LGRYRTLLYGSIAS LGMG V LTA   
Sbjct: 61  NVNGTFVVNVVNIWIGTSNIATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALH 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRP  CNA +  HCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPPHCNADDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFT+ALLIALTGVVYVQTN+SWTLGFAIPT+CFF SI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTIALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           + +PRGSM +D+ARVI++A RKR +S+SS SFYD  M DS+  EKL+HT+RFKWLD+AAI
Sbjct: 241 IVKPRGSMLTDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNP+EELDEQGKPKN WRLCSLQQVEGFKCLVSIIP+WISGIGCF+VFNQPNTFGILQA
Sbjct: 301 IVNPEEELDEQGKPKNSWRLCSLQQVEGFKCLVSIIPVWISGIGCFIVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           +QSNRSIG HFKFPPGWM LAGMI+LSIWIIIYERV IK+ KKITGKERRLTM+QRITIG
Sbjct: 361 IQSNRSIGPHFKFPPGWMSLAGMIALSIWIIIYERVLIKLGKKITGKERRLTMEQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           I++SI  M+ SG+VE++RR+AAL+N  FISP SFA LLPQH LTGLMEAFALVAIMEFFT
Sbjct: 421 ILLSIFSMITSGVVEKHRRDAALKNRLFISPTSFALLLPQHVLTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLT+SVASYLSSLIV +I+ VS + A+S WVGGHDLN+NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTISVASYLSSLIVYVIKKVSAKIAKSPWVGGHDLNQNRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FT+A++  LNLLYFV+FA RFV  YD+KVKL E++ R DL
Sbjct: 541 FTLAVLETLNLLYFVIFARRFVRGYDDKVKLTENVRRNDL 575

BLAST of CmoCh13G009540 vs. ExPASy TrEMBL
Match: A0A5D3BJC6 (Protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003140 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 467/580 (80.52%), Postives = 519/580 (89.48%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDL +PLPSS        PPP ++ GGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 1   MDLVTPLPSS-----QPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           N+NGI+VVNVVNIW GTSN+ATLAGAFIADT LGRYRTLLYGSIAS LGMG V LTA   
Sbjct: 61  NVNGIFVVNVVNIWIGTSNVATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALH 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRP  CN ++  HCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPPHCNVEDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTN+SWTLGFAIPT+CFF SI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           + +PRGSM  D+ARVI++A RKR +S+SS SFYD  M DS+  EKL+HT+RFKWLD+AAI
Sbjct: 241 IVKPRGSMLKDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNP+EELDEQGKPKN WRLCSLQQVEG KCLVSI+P+WISGIGCF+VFNQPNTFGILQA
Sbjct: 301 IVNPEEELDEQGKPKNSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           +QSNRSIG+HFKFPPGWM+LAGMI+LSIWIIIYERV IK+ KK+TGKERRLTM+QRITIG
Sbjct: 361 MQSNRSIGSHFKFPPGWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IV+SI+ M+ SG+VE++RR+AAL+N  FISP SFA LLPQH LTGLMEAFALVA+MEFFT
Sbjct: 421 IVLSILSMITSGVVEKHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLT+SVASYLSSLIV++I+ VSG+ A+S WVGGHDLN NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIA++  LNLLYFV FA RFV  YD+KVKL E+  R DL
Sbjct: 541 FTIAVIETLNLLYFVFFARRFVRGYDDKVKLTENGRRNDL 575

BLAST of CmoCh13G009540 vs. ExPASy TrEMBL
Match: A0A1S3AU85 (protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo OX=3656 GN=LOC103483003 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 467/580 (80.52%), Postives = 519/580 (89.48%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDL +PLPSS        PPP ++ GGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 1   MDLVTPLPSS-----QPPPPPCQKLGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           N+NGI+VVNVVNIW GTSN+ATLAGAFIADT LGRYRTLLYGSIAS LGMG V LTA   
Sbjct: 61  NVNGIFVVNVVNIWIGTSNVATLAGAFIADTRLGRYRTLLYGSIASFLGMGTVALTAALH 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRP  CN ++  HCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPPHCNVEDSGHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTN+SWTLGFAIPT+CFF SI+IFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           + +PRGSM  D+ARVI++A RKR +S+SS SFYD  M DS+  EKL+HT+RFKWLD+AAI
Sbjct: 241 IVKPRGSMLKDVARVIVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNP+EELDEQGKPKN WRLCSLQQVEG KCLVSI+P+WISGIGCF+VFNQPNTFGILQA
Sbjct: 301 IVNPEEELDEQGKPKNSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           +QSNRSIG+HFKFPPGWM+LAGMI+LSIWIIIYERV IK+ KK+TGKERRLTM+QRITIG
Sbjct: 361 MQSNRSIGSHFKFPPGWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IV+SI+ M+ SG+VE++RR+AAL+N  FISP SFA LLPQH LTGLMEAFALVA+MEFFT
Sbjct: 421 IVLSILSMITSGVVEKHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLT+SVASYLSSLIV++I+ VSG+ A+S WVGGHDLN NRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIA++  LNLLYFV FA RFV  YD+KVKL E+  R DL
Sbjct: 541 FTIAVIETLNLLYFVFFARRFVRGYDDKVKLTENGRRNDL 575

BLAST of CmoCh13G009540 vs. NCBI nr
Match: XP_022924109.1 (protein NRT1/ PTR FAMILY 2.8 [Cucurbita moschata])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR
Sbjct: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI
Sbjct: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of CmoCh13G009540 vs. NCBI nr
Match: KAG7019852.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 573/580 (98.79%), Postives = 575/580 (99.14%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 77  MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 136

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMGAVTLTAIF 
Sbjct: 137 NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFH 196

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPS CNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 197 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 256

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPT+CFFFSITIFLLGRHTYI
Sbjct: 257 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 316

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDP MA SSHEEKLVHTERFKWLDKAAI
Sbjct: 317 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPPMAYSSHEEKLVHTERFKWLDKAAI 376

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 377 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 436

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 437 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 496

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 497 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 556

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY
Sbjct: 557 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 616

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 617 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 656

BLAST of CmoCh13G009540 vs. NCBI nr
Match: KAG6584257.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 573/580 (98.79%), Postives = 575/580 (99.14%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 76  MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 135

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMGAVTLTAIF 
Sbjct: 136 NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFH 195

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPS CNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 196 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 255

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPT+CFFFSITIFLLGRHTYI
Sbjct: 256 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 315

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDP MA SSHEEKLVHTERFKWLDKAAI
Sbjct: 316 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPPMAYSSHEEKLVHTERFKWLDKAAI 375

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 376 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 435

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 436 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 495

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 496 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 555

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY
Sbjct: 556 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 615

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 616 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 655

BLAST of CmoCh13G009540 vs. NCBI nr
Match: XP_023520205.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 566/580 (97.59%), Postives = 573/580 (98.79%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHR PPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMGAVTLTAIF 
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPS CNAQNPDHCPQPHLWQLLVLFTGLGLLS+GAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSVGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPT+CFFFSITIFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVII+ACRK RYSVSSYSFYDP MADSSHEEKLVHTERFKWLDKAA 
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKGRYSVSSYSFYDPPMADSSHEEKLVHTERFKWLDKAAF 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           I++SIVCMVVSGIVERYRREAAL+NGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of CmoCh13G009540 vs. NCBI nr
Match: XP_023001041.1 (protein NRT1/ PTR FAMILY 2.8 [Cucurbita maxima])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 571/580 (98.45%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MDLESPLPSSPSNSHHR PPPRRQPGGW AVKYIIGNESFEKLSSMSLISNITVYL+TKY
Sbjct: 1   MDLESPLPSSPSNSHHRPPPPRRQPGGWCAVKYIIGNESFEKLSSMSLISNITVYLTTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NLNGIYVVNVVNIWSGTSN+ATLAGAFIADTCLGRYRTLLYGSIAS LGMGAVTLTAIF 
Sbjct: 61  NLNGIYVVNVVNIWSGTSNVATLAGAFIADTCLGRYRTLLYGSIASFLGMGAVTLTAIFP 120

Query: 121 QLRPSSCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180
           QLRPS CNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK
Sbjct: 121 QLRPSPCNAQNPDHCPQPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGK 180

Query: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYI 240
           SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPT+CFFFSITIFLLGRHTYI
Sbjct: 181 SQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYI 240

Query: 241 MAEPRGSMFSDMARVIISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAI 300
           MAEPRGSMFSDMARVII+ACRKRRYSVSSYSFY+P MADSSHEEKLVHTERFKWLDKAAI
Sbjct: 241 MAEPRGSMFSDMARVIIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAI 300

Query: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360
           IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA
Sbjct: 301 IVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQA 360

Query: 361 LQSNRSIGTHFKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420
           LQSNRSIGTHFKFPPGWM+LAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG
Sbjct: 361 LQSNRSIGTHFKFPPGWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIG 420

Query: 421 IVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480
           I++SIVCMVVSGIVERYRREAAL+NGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT
Sbjct: 421 IILSIVCMVVSGIVERYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFT 480

Query: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYY 540
           MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLI +VSGEFAES WVGGHDLNENRLDYYY
Sbjct: 481 MHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYY 540

Query: 541 FTIAIVGALNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 581
           FTIAIVG LNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL
Sbjct: 541 FTIAIVGTLNLLYFVLFASRFVTSYDNKVKLMEDLNRIDL 580

BLAST of CmoCh13G009540 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 630.9 bits (1626), Expect = 9.8e-181
Identity = 318/566 (56.18%), Postives = 424/566 (74.91%), Query Frame = 0

Query: 1   MDLESPLPSSPSNSHHRHPPPRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKY 60
           MD+ES  PSS       H   +++ GGWRA+KYII NESFEKL+SMSLI N++VYL TKY
Sbjct: 1   MDVESSSPSS-------HALIKKEKGGWRAIKYIIANESFEKLASMSLIGNLSVYLMTKY 60

Query: 61  NLNGIYVVNVVNIWSGTSNIATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFR 120
           NL G+++VNV+NIW G+ NI TLAGAF++D  LGR+ TLL GSIAS +GMG   LTA   
Sbjct: 61  NLGGVFLVNVINIWFGSCNILTLAGAFVSDAYLGRFWTLLLGSIASFIGMGIFALTAALP 120

Query: 121 QLRPSSCNAQNPDHCP-QPHLWQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKG 180
            LRP +C   +P +C  QP  WQL VLF+GLGLL+IGAGG+RPCN+AFGADQFDT+T+KG
Sbjct: 121 SLRPDAC--IDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGADQFDTSTKKG 180

Query: 181 KSQLESFFNWWYLSFTVALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTY 240
           K+ LE+FFNWWY SFTVAL+IALTGVVY+QTNISW +GF IPT C   SIT F++G+HTY
Sbjct: 181 KAHLETFFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTY 240

Query: 241 IMAEPRGSMFSDMARVIISACRKRRYSV-SSYSFYDPSMADSSHEEKLVHTERFKWLDKA 300
           I A+  GS+F+D+ +V+ +AC+KR+    S  +FY     D S    +    R ++ DKA
Sbjct: 241 ICAKAEGSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKA 300

Query: 301 AIIVNPDEELDEQGKPKNPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGIL 360
           +I+ NP+ EL+E G  K  WRLCS+QQV+  KC+ +I+P+W++GI CF++ +Q N +GIL
Sbjct: 301 SIVTNPN-ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGIL 360

Query: 361 QALQSNRSIGTH-FKFPPGWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRI 420
           QA+Q +++ G H F+ P GWM+L  MI+L+IWI +YE V I + K+ITG+++RLT+K RI
Sbjct: 361 QAMQMDKTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRI 420

Query: 421 TIGIVVSIVCMVVSGIVERYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIME 480
              IV+ I+CM+V+G  E+ RR +AL+NGSF+SP+S   LLPQ AL GL EAF+ VA+ME
Sbjct: 421 E--IVMGIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALME 480

Query: 481 FFTMHMPEHMRTVAGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLD 540
           F T+ MPEHMR VAGAIFFL+ S+ASY+ +L++N+I  V+ +  +S W+G  DLN+NRL+
Sbjct: 481 FLTVRMPEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEGKS-WLGDKDLNKNRLE 540

Query: 541 YYYFTIAIVGALNLLYFVLFASRFVT 564
            Y+F IA +   NLLYF LFASR+ T
Sbjct: 541 NYFFIIAGIQVANLLYFRLFASRYAT 553

BLAST of CmoCh13G009540 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 463.4 bits (1191), Expect = 2.7e-130
Identity = 231/539 (42.86%), Postives = 360/539 (66.79%), Query Frame = 0

Query: 23  RQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIAT 82
           ++PGGWRAV +I+GNE+ E+L S+ L++N  VYL+  ++L  +   NV+NIWSG +N+  
Sbjct: 50  KKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTP 109

Query: 83  LAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQ 142
           L GA+I+DT +GR++T+ + S A+LLG+  +TLTA F QL P+SCN+Q+P  C  P+  Q
Sbjct: 110 LVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQ 169

Query: 143 LLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIAL 202
           + VL  GL  LS+G+GGIRPC++ FG DQFD  TE+G   + SFFNW+Y++FTV L+I  
Sbjct: 170 IGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQ 229

Query: 203 TGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRK 262
           T VVY+Q  +SW +GF+IPT     ++ +F  G   Y+  +P GS+FS +A+VI++A +K
Sbjct: 230 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 289

Query: 263 RRYSV-----SSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNP 322
           R+  +      + ++YDP++  SS   KL  + +F+ LDKAA+++  D  L  +G P + 
Sbjct: 290 RKLKLPAEDDGTVTYYDPAI-KSSVLSKLHRSNQFRCLDKAAVVIEGD--LTPEGPPADK 349

Query: 323 WRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPPGW 382
           WRLCS+Q+VE  KCL+ I+PIW +GI          TF + QAL+ +R++G  F+ P G 
Sbjct: 350 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 409

Query: 383 MHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERY 442
           + +  ++++ I++  Y+RVF+   ++ITG +  +T+ QRI  GIV +I  M+V+GIVER 
Sbjct: 410 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 469

Query: 443 RREAALRNG--SFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 502
           RR  ++  G  + ++P+S  +L PQ  L GL EAF ++  +EFF    PEHMR++A ++F
Sbjct: 470 RRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLF 529

Query: 503 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYF 555
            L+ + +SYLSS +V ++   SG      W+   +LN  +LDY+Y+ IA++G +NL+YF
Sbjct: 530 SLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGVVNLVYF 584

BLAST of CmoCh13G009540 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 427.6 bits (1098), Expect = 1.6e-119
Identity = 226/561 (40.29%), Postives = 349/561 (62.21%), Query Frame = 0

Query: 21  PRRQPGGWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNI 80
           P ++ GGWRA+ +I+GNE+ EKL S+ + +N  +YL   +++  +   NV  +W G +N 
Sbjct: 11  PEKKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNF 70

Query: 81  ATLAGAFIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHL 140
           A L GA I+D  +GR++T+ Y S+ S+LG+  VTLTA   QL P  CN  +PD C  P+ 
Sbjct: 71  APLLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNK 130

Query: 141 WQLLVLFTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLI 200
            QL +LF GLG LSIG+GGIRPC++ FG DQFD  TE+G   + SFFNW+YL+ T+ L+ 
Sbjct: 131 LQLGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIF 190

Query: 201 ALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISAC 260
           + T VVY+QT +SW +GF+IPT     ++ +F +G   Y+  +P GS+FS +ARVI++A 
Sbjct: 191 SHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAAR 250

Query: 261 RKRRYSVS-----SYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 320
           +KR   +S     +  +Y+P +       KL  T++FK+LDKAA+I+  D +L  +G P 
Sbjct: 251 KKRDLKISLVDDGTEEYYEPPVKPGV-LSKLPLTDQFKFLDKAAVIL--DGDLTSEGVPA 310

Query: 321 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 380
           N WRLCS+Q+VE  KCL+ ++P+W +GI   V      TF + QA + +R +G HF+ P 
Sbjct: 311 NKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPA 370

Query: 381 GWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVE 440
             + +   I++ IW+ IYE + +    ++  ++ R+T+ QR+ IGIV +I+ M  +G VE
Sbjct: 371 ASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVE 430

Query: 441 RYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 500
             RR  A      ++ +S  +L     L GL E+F  + ++EFF    PEHMR++A ++F
Sbjct: 431 GVRRTRATE----MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLF 490

Query: 501 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFV 560
            L+ + A+YLSSL+V  +  VSG      W+   DL+  +LDY+Y+ IA++G +NL+YF 
Sbjct: 491 PLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFW 550

Query: 561 LFASRFVTSYDNKVKLMEDLN 577
             A R+     +++   ED N
Sbjct: 551 YCAHRYQYKAGSQI---EDFN 557

BLAST of CmoCh13G009540 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 8.5e-116
Identity = 218/540 (40.37%), Postives = 337/540 (62.41%), Query Frame = 0

Query: 27  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIATLAGA 86
           GW+ + +IIGNE+FEKL  +   SN+ +YL+T +N+  I    VVNI+ GTSN  T+  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 87  FIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQLLVL 146
           F+ D+  GRY+TL +  IA  LG  A+ LTA+   L P+ C  +    C  P + Q++ L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 147 FTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIALTGVV 206
              + LL IGAGGIRPCN+ FGADQFD  T++GK  +ESFFNW++ +FT A +++LT +V
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLIV 201

Query: 207 YVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRKRRYS 266
           YVQ+N+SW++G AIP +       IF  G   Y+  +  GS    + RVI+ A +KRR  
Sbjct: 202 YVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRLK 261

Query: 267 VSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNPWRLCSLQQV 326
               +     +A      KL HTE+F++LDK+A I   D++L++ G P + W+LCS+QQV
Sbjct: 262 PVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQV 321

Query: 327 EGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGT-HFKFPPGWMHLAGMIS 386
           E  KC++ ++P+W+S    ++ + Q  T+ I Q+LQS+R +G   F+ P G   +  M+ 
Sbjct: 322 EEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLMLG 381

Query: 387 LSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERYRREAAL-- 446
           ++I+I IY+RV +   +K TG++  +T  QR+  G+ + I  M+VS IVE+YRR+ AL  
Sbjct: 382 MTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALTK 441

Query: 447 -------RNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIFF 506
                  R G+ IS +S  +L+PQ  L G+ +A A V  MEF+    PE+MR+ AG++++
Sbjct: 442 PTLGLAPRKGA-ISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 507 LTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFVL 557
             + +ASYLS+ +++ +   +  F+  +W+   DLN+ RL+Y+YF +A +  LNL YF+L
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWL-PEDLNKGRLEYFYFLVAGMMTLNLAYFLL 558

BLAST of CmoCh13G009540 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 409.5 bits (1051), Expect = 4.6e-114
Identity = 223/575 (38.78%), Postives = 344/575 (59.83%), Query Frame = 0

Query: 27  GWRAVKYIIGNESFEKLSSMSLISNITVYLSTKYNLNGIYVVNVVNIWSGTSNIATLAGA 86
           GW+ + +IIGNE+FEKL  +  +SN+ VYL+  +NL  I    ++N +SGT N  T   A
Sbjct: 46  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAA 105

Query: 87  FIADTCLGRYRTLLYGSIASLLGMGAVTLTAIFRQLRPSSCNAQNPDHCPQPHLWQLLVL 146
           F+ DT  GRY+TL    IA  LG   + LTA   QL P++C       C  P   Q+  L
Sbjct: 106 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFL 165

Query: 147 FTGLGLLSIGAGGIRPCNVAFGADQFDTTTEKGKSQLESFFNWWYLSFTVALLIALTGVV 206
             GLG L +GAGGIRPCN+AFGADQF+  +E GK  ++SFFNW++ +FT A +++LT VV
Sbjct: 166 LMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVV 225

Query: 207 YVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARVIISACRKRRYS 266
           YVQ+N+SWT+G  IP +  F +  IF  G   Y+  +  GS  + +A+VI  A +KR   
Sbjct: 226 YVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLK 285

Query: 267 ------VSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPKNPWRL 326
                 ++ Y++Y P  A+S    KL +T++F++LDKAAI+  P+++L   GKP +PW+L
Sbjct: 286 PAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQPDGKPADPWKL 345

Query: 327 CSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGT-HFKFPPGWMH 386
           C++QQVE  KC+V ++PIW +    ++   Q  T+ + QALQS+R +G+  F  P     
Sbjct: 346 CTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYV 405

Query: 387 LAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVERYRR 446
           +  M  ++++I++Y+RV +   ++ITG +  +T+ QRI  GI  +   +VV+G VE  RR
Sbjct: 406 VFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRR 465

Query: 447 EAAL---------RNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTV 506
             AL         R G  IS +S  +L+PQ +L G+ EAFA +  MEF+    PE+MR+ 
Sbjct: 466 TFALTKPTLGMAPRKGE-ISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSF 525

Query: 507 AGAIFFLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALN 566
           AG+IF++   V+SYL S ++  +   +   +   W+   DLN+ RLD +YF IA + A+N
Sbjct: 526 AGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYFMIAGILAVN 585

Query: 567 LLYFVLFASRF--------VTSYDNKVKLMEDLNR 578
             YF++ +  +        VT+Y+    +++  ++
Sbjct: 586 FAYFLVMSRWYRYKGSDDEVTTYETNENIIKQQDK 613

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E8X31.4e-17956.18Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2[more]
Q8RX773.8e-12942.86Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX92.3e-11840.29Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Q9M9V71.2e-11440.37Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1[more]
Q9LV106.5e-11338.78Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1E8860.0e+00100.00protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita moschata OX=3662 GN=LOC111431644 PE=3 ... [more]
A0A6J1KHH50.0e+0096.72protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita maxima OX=3661 GN=LOC111495296 PE=3 SV... [more]
A0A0A0LQR53.0e-27080.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G045580 PE=3 SV=1[more]
A0A5D3BJC61.1e-26980.52Protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AU851.1e-26980.52protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo OX=3656 GN=LOC103483003 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022924109.10.0e+00100.00protein NRT1/ PTR FAMILY 2.8 [Cucurbita moschata][more]
KAG7019852.10.0e+0098.79Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
KAG6584257.10.0e+0098.79Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023520205.10.0e+0097.59protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo][more]
XP_023001041.10.0e+0096.72protein NRT1/ PTR FAMILY 2.8 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G28470.19.8e-18156.18Major facilitator superfamily protein [more]
AT1G69870.12.7e-13042.86nitrate transporter 1.7 [more]
AT1G27080.11.6e-11940.29nitrate transporter 1.6 [more]
AT1G18880.18.5e-11640.37Major facilitator superfamily protein [more]
AT5G62680.14.6e-11438.78Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..525
e-value: 4.8E-76
score: 256.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 19..565
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 8..569
e-value: 1.4E-157
score: 527.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 43..556
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR11654:SF181PROTEIN NRT1/ PTR FAMILY 2.8coord: 19..565
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 32..561
e-value: 0.0
score: 523.753

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G009540.1CmoCh13G009540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity