CmoCh13G005050 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G005050
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionFAD-binding Berberine family protein
LocationCmo_Chr13: 5983604 .. 5985655 (-)
RNA-Seq ExpressionCmoCh13G005050
SyntenyCmoCh13G005050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACATTAAAGTCCTCAGTAATTTTGGTGCTATCATTTTCATTTCTTCTGGTTTTCGCTTCTGCAAAAACAGCTACTGAAAATTTCGTTCAATGTCTTCTCCGCCGCTCTCCGCCGTCCTACCCTATTGCTCCGGCGATCTTTACACCCAACAATCATTCTTATTCCTCTGTTCTTCAGACTTACATTCAAAACTTGAGATTCAACACTTCAACAACTCCTAAGCCATTCCTTATTCTCACAGCTCTCCATGAATATCATGTTAGAATTGCAATTCTATGTGCTCGAATCGATAATCTTCTGATGAAGATTCGTAGCGGCGGCCACGATTACGAGGGAGTTTCTTACGTCTCCGACATTGTCCCTTTCTTCATTCTTGATATGTTCAACCTCCGAAGAATCTCTATTGATATAAAAACCGAGACGGCTTGGGTATTTTTATCGATACTCATTTACTTTATTAGGAATCACGACTCTTCACAATAGCATGGTATTGTCAACTTTGTGCATAAGTTCGCAATGGAGATAGTATTCCTTGCTTATAAACCGATGATCATTCCCTGAATTAACCAACGTGGGACTCCCTTCCAACCATTCTCGACGCACTTCACTCATGGGCTTGAAAATTAGGATAGGATTACGAGCGAGAATAATGTTAATTAAAAAAAAAATGAACATGACATCATTTTTAAGAAACTTTTGACATCATTTATTTATATTCTTTATGATCAGGTTCAAACAGGAGCCACCATAGGAGAAGTGTTTTATAAAATTGCAGAGGAAAGCAAGCTCCATGGCTTCCCGGCCGGAGTGTGCCCTACGATGGGTGTCGGTGGCCATTTGAGCGGTGGCGGGTACGGTAACATGATGAGGAAACATGGCCTCACGGTGGATCAATTCATCGACGCGAAGATAATCGACGTAAACGGGAAGCTTCTCGACCGAAAAGCAATGGGGGAGGATCTATTCTGGGCCATCACAGGCGGCGGAGGATCAAGCTTCGGAGTCGTTGTAGCGTACAAAATCCGATTGGTTCGTGTACCGGAGACGGTAACGGTGTTCAGAGTAAGAAGAACGTTGGAAGAGAATGAGTTGACGGAGATAGTAGATGAGTGGCAGCGGGTGGCACATGTGATTGACAATGATTTGTTCATAAGGGTTACGTTTGATGTTGTAAATGGAACACACAAGGGAAACAAGACACTGAGAGCAACGTTTCTAGCCATGTTCCTCGGAGATTCCCAAACCCTTTTGTCTCTCATCAACAACAGCTTCCCTAAATTGGGTTTGAAGAAACCTGATTGCCTTGAAATGAGTTGGATTGAATCAGTGCTTTTTTGGACAGATGGTAAACTCCCTTTTTACTCCCAAATTTATGATCAAATTTTATCGAAATGGAAGCTTACGGTTTCATGTTTTATCAAATTTTATCGAAATTTCATTCAAATATATCGATTTTTCTTGGAAGCTTACGGTTTCATGTTTTATTCCACTCCCTGATCGAGTTACATAGAAACATCTGTGAATGCCCTATTGAACCGAATGACGGGACGGATCCACCTAAAAAGAAAGTCGGACTATGTGAAAACCCTAATTCCAAAGAAAGGATTTAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGAAATTCAACCCCTATGGTGGAAGAATGGGTGAGATTGCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATCCAGTACACAACAAACTGGAATGAAGAAGGAAGCCGACAAGCCGACCATTTGATAGACTTGACGAGGAAACTTCACAAGTTTATGACACCCTTTGTGTCCAAGAATCCAAGAACAGCGTTTTTGAACTATAGAGATCTTGATATTGGTGTAACCCATAATGGGAAGAAGAGTTACTATGAAGGGAGGGGCTATGGAATCAAATATTTTGAAGGCAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTGACAACTTTTTTAGGAATGAACAAAGCATTCCAAGATTTCCACATGTTTGA

mRNA sequence

ATGAAAACATTAAAGTCCTCAGTAATTTTGGTGCTATCATTTTCATTTCTTCTGGTTTTCGCTTCTGCAAAAACAGCTACTGAAAATTTCGTTCAATGTCTTCTCCGCCGCTCTCCGCCGTCCTACCCTATTGCTCCGGCGATCTTTACACCCAACAATCATTCTTATTCCTCTGTTCTTCAGACTTACATTCAAAACTTGAGATTCAACACTTCAACAACTCCTAAGCCATTCCTTATTCTCACAGCTCTCCATGAATATCATGTTAGAATTGCAATTCTATGTGCTCGAATCGATAATCTTCTGATGAAGATTCGTAGCGGCGGCCACGATTACGAGGGAGTTTCTTACGTCTCCGACATTGTCCCTTTCTTCATTCTTGATATGTTCAACCTCCGAAGAATCTCTATTGATATAAAAACCGAGACGGCTTGGGTTCAAACAGGAGCCACCATAGGAGAAGTGTTTTATAAAATTGCAGAGGAAAGCAAGCTCCATGGCTTCCCGGCCGGAGTGTGCCCTACGATGGGTGTCGGTGGCCATTTGAGCGGTGGCGGGTACGGTAACATGATGAGGAAACATGGCCTCACGGTGGATCAATTCATCGACGCGAAGATAATCGACGTAAACGGGAAGCTTCTCGACCGAAAAGCAATGGGGGAGGATCTATTCTGGGCCATCACAGGCGGCGGAGGATCAAGCTTCGGAGTCGTTGTAGCGTACAAAATCCGATTGGTTCGTGTACCGGAGACGGTAACGGTGTTCAGAGTAAGAAGAACGTTGGAAGAGAATGAGTTGACGGAGATAGTAGATGAGTGGCAGCGGGTGGCACATGTGATTGACAATGATTTGTTCATAAGGGTTACGTTTGATGTTGTAAATGGAACACACAAGGGAAACAAGACACTGAGAGCAACGTTTCTAGCCATGTTCCTCGGAGATTCCCAAACCCTTTTGTCTCTCATCAACAACAGCTTCCCTAAATTGGGTTTGAAGAAACCTGATTGCCTTGAAATGAGTTGGATTGAATCAGTGCTTTTTTGGACAGATGAAACATCTGTGAATGCCCTATTGAACCGAATGACGGGACGGATCCACCTAAAAAGAAAGTCGGACTATGTGAAAACCCTAATTCCAAAGAAAGGATTTAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGAAATTCAACCCCTATGGTGGAAGAATGGGTGAGATTGCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATCCAGTACACAACAAACTGGAATGAAGAAGGAAGCCGACAAGCCGACCATTTGATAGACTTGACGAGGAAACTTCACAAGTTTATGACACCCTTTGTGTCCAAGAATCCAAGAACAGCGTTTTTGAACTATAGAGATCTTGATATTGGTGTAACCCATAATGGGAAGAAGAGTTACTATGAAGGGAGGGGCTATGGAATCAAATATTTTGAAGGCAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTGACAACTTTTTTAGGAATGAACAAAGCATTCCAAGATTTCCACATGTTTGA

Coding sequence (CDS)

ATGAAAACATTAAAGTCCTCAGTAATTTTGGTGCTATCATTTTCATTTCTTCTGGTTTTCGCTTCTGCAAAAACAGCTACTGAAAATTTCGTTCAATGTCTTCTCCGCCGCTCTCCGCCGTCCTACCCTATTGCTCCGGCGATCTTTACACCCAACAATCATTCTTATTCCTCTGTTCTTCAGACTTACATTCAAAACTTGAGATTCAACACTTCAACAACTCCTAAGCCATTCCTTATTCTCACAGCTCTCCATGAATATCATGTTAGAATTGCAATTCTATGTGCTCGAATCGATAATCTTCTGATGAAGATTCGTAGCGGCGGCCACGATTACGAGGGAGTTTCTTACGTCTCCGACATTGTCCCTTTCTTCATTCTTGATATGTTCAACCTCCGAAGAATCTCTATTGATATAAAAACCGAGACGGCTTGGGTTCAAACAGGAGCCACCATAGGAGAAGTGTTTTATAAAATTGCAGAGGAAAGCAAGCTCCATGGCTTCCCGGCCGGAGTGTGCCCTACGATGGGTGTCGGTGGCCATTTGAGCGGTGGCGGGTACGGTAACATGATGAGGAAACATGGCCTCACGGTGGATCAATTCATCGACGCGAAGATAATCGACGTAAACGGGAAGCTTCTCGACCGAAAAGCAATGGGGGAGGATCTATTCTGGGCCATCACAGGCGGCGGAGGATCAAGCTTCGGAGTCGTTGTAGCGTACAAAATCCGATTGGTTCGTGTACCGGAGACGGTAACGGTGTTCAGAGTAAGAAGAACGTTGGAAGAGAATGAGTTGACGGAGATAGTAGATGAGTGGCAGCGGGTGGCACATGTGATTGACAATGATTTGTTCATAAGGGTTACGTTTGATGTTGTAAATGGAACACACAAGGGAAACAAGACACTGAGAGCAACGTTTCTAGCCATGTTCCTCGGAGATTCCCAAACCCTTTTGTCTCTCATCAACAACAGCTTCCCTAAATTGGGTTTGAAGAAACCTGATTGCCTTGAAATGAGTTGGATTGAATCAGTGCTTTTTTGGACAGATGAAACATCTGTGAATGCCCTATTGAACCGAATGACGGGACGGATCCACCTAAAAAGAAAGTCGGACTATGTGAAAACCCTAATTCCAAAGAAAGGATTTAACAAAATATGGAAGAAAATGATAGAACTTGAAAAACCCATGATGAAATTCAACCCCTATGGTGGAAGAATGGGTGAGATTGCATCAAATGCAACTCCATTTCCTCATAGAGCTGGAAACTTATGGAAGATCCAGTACACAACAAACTGGAATGAAGAAGGAAGCCGACAAGCCGACCATTTGATAGACTTGACGAGGAAACTTCACAAGTTTATGACACCCTTTGTGTCCAAGAATCCAAGAACAGCGTTTTTGAACTATAGAGATCTTGATATTGGTGTAACCCATAATGGGAAGAAGAGTTACTATGAAGGGAGGGGCTATGGAATCAAATATTTTGAAGGCAACTTTGAGAGGTTGGTGAAGATCAAGACCAAGGTTGATCCTGACAACTTTTTTAGGAATGAACAAAGCATTCCAAGATTTCCACATGTTTGA

Protein sequence

MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNRMTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV
Homology
BLAST of CmoCh13G005050 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 9.6e-188
Identity = 330/530 (62.26%), Postives = 404/530 (76.23%), Query Frame = 0

Query: 4   LKSSVILVLSF-SFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVLQT 63
           +K ++ILVL F  F+   +S+   +E F QCL   S P +PI+PAIF   N SYSSVLQ 
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQA 60

Query: 64  YIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS-DI 123
            I+NLRFNT++TPKPFLI+ A HE HV+ AI C +  NL MKIRSGGHDY+G+SYV+   
Sbjct: 61  NIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 120

Query: 124 VPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGH 183
            PFF+LDMFNLR + +D+ ++TAWVQTGA +GEV+Y I E+SK   +PAG+CPT+GVGGH
Sbjct: 121 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 180

Query: 184 LSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAY 243
           +SGGGYGNMMRK+GLTVD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AY
Sbjct: 181 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 240

Query: 244 KIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNK 303
           KI LV VPE VTVFR+ RTLE+N  T+I+  WQ+VA  + ++LFIR   DVVNGT    K
Sbjct: 241 KINLVEVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK 300

Query: 304 TLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALL 363
           T+R TF+AMFLGD+ TLLS++N  FP+LGL + DC E SWI+SVLFWT+    +S   LL
Sbjct: 301 TVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLL 360

Query: 364 NRMTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFP 423
            R     +LKRKSDYV+  I + G   IWKKMIELE P M FNPYGG MG I+S  TPFP
Sbjct: 361 QRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFP 420

Query: 424 HRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGV- 483
           +RAGNLWKIQY  NW +E     D  ++LTRKL++FMTPFVSKNPR +F NYRD+D+G+ 
Sbjct: 421 YRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGIN 480

Query: 484 THNGK-KSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFP 527
           +HNGK  SY EG+ YG KYF GNFERLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 481 SHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of CmoCh13G005050 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 285/531 (53.67%), Postives = 375/531 (70.62%), Query Frame = 0

Query: 9   ILVLSFSFLLV----FASAKTAT-----ENFVQCLL-RRSPPSYPIAPAIFTPNNHSYSS 68
           + V  F F LV    F+SA   +     E+FVQC   +   P   I   +F+  N S+SS
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 67

Query: 69  VLQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYV 128
           VL+ YI+N RFNTS+TPKP +I+T   + HV  A+ C++  N L+KIRSGGHDYEG+SY+
Sbjct: 68  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 127

Query: 129 SDIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGV 188
           SD  PFFILDM NLR +S+DI  ++AW+  GAT+GEV+Y+I E+SK+HGFPAGVCPT+GV
Sbjct: 128 SD-KPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGV 187

Query: 189 GGHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVV 248
           GGH+SGGGYGNM+RK GL+VD  IDAKI+DVNG++LDRK+MGEDLFWAI+GGGG+SFGVV
Sbjct: 188 GGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVV 247

Query: 249 VAYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHK 308
           + YK++LV VPETVTVFRV + ++   + ++V +WQ V    D +LF+R+    V  T K
Sbjct: 248 LGYKVKLVPVPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRK 307

Query: 309 GNKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNAL- 368
             KT+RAT +A+FLG ++ +++L+   FP+L LKK +C EM+W +S L+W +  +   + 
Sbjct: 308 KVKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQID 367

Query: 369 ----LNRMTGRIHL-KRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIAS 428
               L+R   R +  KRKSDYV + IP+ G   ++KKM EL K  + FNPYGG+M E+  
Sbjct: 368 PKVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTV 427

Query: 429 NATPFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRD 488
           NATPFPHR+  L+KIQY+  W E         ++    L+ FMT FVSKNPR A+LNYRD
Sbjct: 428 NATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRD 487

Query: 489 LDIGVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
           +DIGV  +G  SY EG  YG KYF  NF+RLVK+KT  DPDNFFRNEQSIP
Sbjct: 488 VDIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of CmoCh13G005050 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 545.0 bits (1403), Expect = 9.1e-154
Identity = 273/511 (53.42%), Postives = 364/511 (71.23%), Query Frame = 0

Query: 20  FASAKTATENFVQCLL-RRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLRFNTSTTPKPF 79
           F+ + +   +F++C   +   P   I   +F+  N ++SSVL+ YI+N RFNTS+T KP 
Sbjct: 29  FSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNTSSTLKPT 88

Query: 80  LILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILDMFNLRRISID 139
           +I+T   E HV  A+ C++  N L+KIRSGGHDY+G+SY+SD  PFFILDM N+R +S+D
Sbjct: 89  IIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILDMSNIRDVSVD 148

Query: 140 IKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYGNMMRKHGLTV 199
           I + +AW+  GAT+GEV+Y+I E+S++HGFPAGVCPT+GVGGHLSGGGYGNM+RK GL+V
Sbjct: 149 IASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGNMVRKFGLSV 208

Query: 200 DQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRVPETVTVFRVR 259
           D   DAKI+DVNG++LDRKAMGEDLFWAITGGGG S+GVV+ YK++LV VP  VTVFRV 
Sbjct: 209 DYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVVTVFRVE 268

Query: 260 RTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFLAMFLGDSQTL 319
           + ++   + ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +A+FLG +  +
Sbjct: 269 QYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVVALFLGRADEV 328

Query: 320 LSLINNSFPKLGLKKPDCLEMSWIESVLFWTD-----ETSVNALLNRMTGRIHL-KRKSD 379
           ++L++  FP+LGLKK +C EM+W +S L+W +     +      L+R        KRKSD
Sbjct: 329 VALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKRKSD 388

Query: 380 YVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNLWKIQYTTN 439
           YV T IPKKG   ++KKMIEL K  + FNPYGG+M E+A NA PFPHR   L+KIQY+ N
Sbjct: 389 YVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQYSVN 448

Query: 440 WNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKSYYEGRGYG 499
           W E  +      ++  + L+ FMT FVSKNPR+++ NYRD+DIGV  +G  SY EG  YG
Sbjct: 449 WKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGEVYG 508

Query: 500 IKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
            KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 509 RKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CmoCh13G005050 vs. ExPASy Swiss-Prot
Match: Q9SA87 (Berberine bridge enzyme-like 10 OS=Arabidopsis thaliana OX=3702 GN=At1g30720 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 265/525 (50.48%), Postives = 371/525 (70.67%), Query Frame = 0

Query: 11  VLSFSFLLVFASAKTATEN------FVQCLLRR-SPPSYPIAPAIFTPNNHSYSSVLQTY 70
           +L  S LL+ +++ T +++      F++CL R+ + P+ P +   + P N S+++VL++ 
Sbjct: 4   LLVISLLLLISTSVTTSQSVTDPIAFLRCLDRQPTDPTSPNSAVAYIPTNSSFTTVLRSR 63

Query: 71  IQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVP 130
           I NLRF+  TTPKP  ++ A    H++ A+ CAR  +L ++IRSGGHD+EG+SY S  VP
Sbjct: 64  IPNLRFDKPTTPKPISVVAAATWTHIQAAVGCARELSLQVRIRSGGHDFEGLSYTS-TVP 123

Query: 131 FFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLS 190
           FF+LDMF  + + +++   TAWV +GAT+GE++Y+I+E+S + GFPAG+  T+GVGGH S
Sbjct: 124 FFVLDMFGFKTVDVNLTERTAWVDSGATLGELYYRISEKSNVLGFPAGLSTTLGVGGHFS 183

Query: 191 GGGYGNMMRKHGLTVDQFIDAKIIDVNGKLL-DRKAMGEDLFWAITGGGGSSFGVVVAYK 250
           GGGYGN+MRK+GL+VD    + I+D NG +  DR +MGED FWAI GGG +S+GVV+ YK
Sbjct: 184 GGGYGNLMRKYGLSVDNVFGSGIVDSNGNIFTDRVSMGEDRFWAIRGGGAASYGVVLGYK 243

Query: 251 IRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKT 310
           I+LV VPE VTVF+V +T+ E  + +++ +WQ  AH  D +LF+R+T  +VNGT  G  T
Sbjct: 244 IQLVPVPEKVTVFKVGKTVGEGAV-DLIMKWQSFAHSTDRNLFVRLTLTLVNGTKPGENT 303

Query: 311 LRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALLN 370
           + ATF+ M+LG S  LL+++N  FP+L LKK DC EM WI+SVLFW D    T  + LLN
Sbjct: 304 VLATFIGMYLGRSDKLLTVMNRDFPELKLKKTDCTEMRWIDSVLFWDDYPVGTPTSVLLN 363

Query: 371 RMTG-RIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFP 430
            +   ++ +KRKSDYVK LI +     I KK++E+EK  M +NPYGGRMGEI S+ TPFP
Sbjct: 364 PLVAKKLFMKRKSDYVKRLISRTDLGLILKKLVEVEKVKMNWNPYGGRMGEIPSSRTPFP 423

Query: 431 HRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVT 490
           HRAGNL+ I+Y  +W+E G       + L  + ++FMTP+VS NPR AFLNYRDLDIG  
Sbjct: 424 HRAGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAFLNYRDLDIG-- 483

Query: 491 HNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
            + K +Y EG+ YG KYF+ NFERLV IKT +D +NF++NEQSIP
Sbjct: 484 SSVKSTYQEGKIYGAKYFKENFERLVDIKTTIDAENFWKNEQSIP 524

BLAST of CmoCh13G005050 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.3e-148
Identity = 277/534 (51.87%), Postives = 368/534 (68.91%), Query Frame = 0

Query: 4   LKSSVILVLSFS-FLLV----FASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSS 63
           +KSS    L F+ FLL+    FA+     ++F QC +    PS PI    +T  N ++ +
Sbjct: 1   MKSSTTQTLIFTVFLLLIPTSFAAPPKLKDSFTQC-VTVFKPSVPIQNFTYTQQNPNFLT 60

Query: 64  VLQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYV 123
           +L  Y++NLR+   TT KP  I+ A H  H++  I CA+   L ++IRSGGHDY+G+SY+
Sbjct: 61  ILNNYVRNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYL 120

Query: 124 SDIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEES-KLHGFPAGVCPTMG 183
           S  V F +LDMFNLR I ID K +TAWVQ+GAT+GE++Y +A +S  L GFPAG+CP +G
Sbjct: 121 S-TVDFVVLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLG 180

Query: 184 VGGHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGV 243
            GGH SGGGYGNMMRK+GL++D  IDAKI+D N ++LDR +MGEDLFWA+ GGG +SF V
Sbjct: 181 AGGHFSGGGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCV 240

Query: 244 VVAYKIRLVRVPETVTVFRVRRTLEENEL-TEIVDEWQRVAHVIDNDLFIRVTFDVVNGT 303
           V+A+KI+LV VPE VTVF V        + T++  +WQ +A  IDNDLFIR+T       
Sbjct: 241 VLAWKIKLVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLS----- 300

Query: 304 HKGNKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFW------TD 363
              NKT++A+F+ M+LG+S+ LL ++N  FP+LGL K +C+EM WIESVLFW      T 
Sbjct: 301 -SSNKTVKASFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTA 360

Query: 364 ETSVNALLNRMTGR-IHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGE 423
            TSV  +LNR+  + I+LKRKSDYV+  I K G   I+K + E E   M +NPYGGRM E
Sbjct: 361 PTSV--MLNRIPQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSE 420

Query: 424 IASNATPFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLN 483
           I +  T FPHRAGN++KIQY++NW   G   A   +  T ++ + M+P+VSKNPR AFLN
Sbjct: 421 IPATETAFPHRAGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLN 480

Query: 484 YRDLDIGVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
           YRD+DIG   N   +Y EG+ YG+KYF+ NFERLV++KT+VDPDN FR EQSIP
Sbjct: 481 YRDIDIG--KNLNSTYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of CmoCh13G005050 vs. ExPASy TrEMBL
Match: A0A6J1ENY7 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111434269 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.8e-309
Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60
           MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL
Sbjct: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60

Query: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120
           QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD
Sbjct: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120

Query: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180
           IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG
Sbjct: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180

Query: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240
           HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA
Sbjct: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240

Query: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300
           YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN
Sbjct: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300

Query: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR 360
           KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR
Sbjct: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR 360

Query: 361 MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420
           MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR
Sbjct: 361 MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420

Query: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480
           AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN
Sbjct: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480

Query: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV
Sbjct: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 528

BLAST of CmoCh13G005050 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.0e-256
Identity = 441/533 (82.74%), Postives = 482/533 (90.43%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASA-KTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSV 60
           M+ L+SS IL LSFSFL+ F+SA  +A ENF+QCLLR SPPSYPIAPAIFTPNN SYSSV
Sbjct: 1   MQILRSS-ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSV 60

Query: 61  LQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS 120
           L+TYI+NLRFNTSTTPKPFLILTALHE HV++AILCARI NL MKIRSGGHDYEGVSYVS
Sbjct: 61  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVS 120

Query: 121 DIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVG 180
           D+VPFF+LDMFNLR I++D KTETAWVQTGAT+GEV+Y++AE++K +GFPAGVCPT+GVG
Sbjct: 121 DVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVG 180

Query: 181 GHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVV 240
           GH  GGGYGNMMRKHGL+VDQ IDAKIIDVNGKLLDRKAMGEDLFWAI GGGGSSFGVVV
Sbjct: 181 GHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 240

Query: 241 AYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKG 300
           AYKIR+VRVPE VTVFRV+RTLE+NELTEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT K 
Sbjct: 241 AYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKE 300

Query: 301 NKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNA 360
            KTLRATF+A+FLGDS  LL +INNSFPKLGLKK DC+EMSW++SVLFWT+    TSV A
Sbjct: 301 KKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 360

Query: 361 LLNRMTGRI-HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNAT 420
           LL+R    + HLKRKSDYVK  IPK+G +KIWKKMIELEKPMM FNPYGGRM EI SNAT
Sbjct: 361 LLSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNAT 420

Query: 421 PFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDI 480
           PFPHRAGNLWKIQY TNW+EEG R+A H IDLTRKL+KFMTPFVSKNPRTAFLNYRDLDI
Sbjct: 421 PFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 480

Query: 481 GVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GV HNGK SYYEGR YGIKYFEGNFERLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 GVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CmoCh13G005050 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.0e-256
Identity = 441/533 (82.74%), Postives = 482/533 (90.43%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASA-KTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSV 60
           M+ L+SS IL LSFSFL+ F+SA  +A ENF+QCLLR SPPSYPIAPAIFTPNN SYSSV
Sbjct: 1   MQILRSS-ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSV 60

Query: 61  LQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS 120
           L+TYI+NLRFNTSTTPKPFLILTALHE HV++AILCARI NL MKIRSGGHDYEGVSYVS
Sbjct: 61  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVS 120

Query: 121 DIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVG 180
           D+VPFF+LDMFNLR I++D KTETAWVQTGAT+GEV+Y++AE++K +GFPAGVCPT+GVG
Sbjct: 121 DVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVG 180

Query: 181 GHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVV 240
           GH  GGGYGNMMRKHGL+VDQ IDAKIIDVNGKLLDRKAMGEDLFWAI GGGGSSFGVVV
Sbjct: 181 GHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 240

Query: 241 AYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKG 300
           AYKIR+VRVPE VTVFRV+RTLE+NELTEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT K 
Sbjct: 241 AYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKE 300

Query: 301 NKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNA 360
            KTLRATF+A+FLGDS  LL +INNSFPKLGLKK DC+EMSW++SVLFWT+    TSV A
Sbjct: 301 KKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 360

Query: 361 LLNRMTGRI-HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNAT 420
           LL+R    + HLKRKSDYVK  IPK+G +KIWKKMIELEKPMM FNPYGGRM EI SNAT
Sbjct: 361 LLSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNAT 420

Query: 421 PFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDI 480
           PFPHRAGNLWKIQY TNW+EEG R+A H IDLTRKL+KFMTPFVSKNPRTAFLNYRDLDI
Sbjct: 421 PFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 480

Query: 481 GVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GV HNGK SYYEGR YGIKYFEGNFERLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 GVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CmoCh13G005050 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.2e-251
Identity = 426/522 (81.61%), Postives = 474/522 (90.80%), Query Frame = 0

Query: 11  VLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLRFN 70
           +LSFS LL FASA TA ENF+QCLLR SPPSYPIAPAIFT NN SYSSVL+TYI+NLRFN
Sbjct: 12  LLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLETYIRNLRFN 71

Query: 71  TSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILDMF 130
           TSTTPKPFLILTALHE HV++AILCARI +L MKIRSGGHDYEGVSYVSD+VPFF+LDMF
Sbjct: 72  TSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDVVPFFVLDMF 131

Query: 131 NLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYGNM 190
           NLR IS+D+KTETAWVQTGAT+GEV+Y+IAE+ K HGFPAGVCPT+GVGGH  GGGYGNM
Sbjct: 132 NLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGHFGGGGYGNM 191

Query: 191 MRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRVPE 250
           MRK+GL+VDQ IDAKI+DVNG LLDRKAMGEDLFWAI GGGGSSFGVVVAYKIR VRVPE
Sbjct: 192 MRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRTVRVPE 251

Query: 251 TVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFLAM 310
           TVTVFRV RTLE+N++TEIVD+WQRVAHVID DLFIRVTFDVVNG+  G KTLRATF+A+
Sbjct: 252 TVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKKTLRATFIAL 311

Query: 311 FLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALLNRMTGRI-H 370
           FLGDS+ LLS++N SFPKLGLKK DCLEMSW++SVLFWT+    TSV+ LL+R    + H
Sbjct: 312 FLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDELLSRTPQVLTH 371

Query: 371 LKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNLWK 430
           LKRKSDYVKTLIPK+  N+IWKKMIELEKP M FNPYGGRM EI SNATPFPHRAGNLWK
Sbjct: 372 LKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPFPHRAGNLWK 431

Query: 431 IQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKSYY 490
           IQY TNW+E+G++QA+H IDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIGV HNGK+SYY
Sbjct: 432 IQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGVNHNGKRSYY 491

Query: 491 EGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           EGR +GIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 492 EGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYV 533

BLAST of CmoCh13G005050 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.3e-251
Identity = 426/524 (81.30%), Postives = 474/524 (90.46%), Query Frame = 0

Query: 9   ILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLR 68
           I +LSFS LL FASA TA ENF+QCLLR SPPSYPIAPAIFT NN SYSSVL+TYI+NLR
Sbjct: 10  IFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLETYIRNLR 69

Query: 69  FNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILD 128
           FNTSTTPKPFLILTALHE HV++AILCARI +L MKIRSGGHDYEGVSYVSD+VPFF+LD
Sbjct: 70  FNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDVVPFFVLD 129

Query: 129 MFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYG 188
           MFNLR IS+D+KTETAWVQTGAT+GEV+Y+IAE+ K HGFPAGVCPT+GVGGH  GGGYG
Sbjct: 130 MFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGHFGGGGYG 189

Query: 189 NMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRV 248
           NMMRK+GL+VDQ IDAKI+DV G LLDRKAMGEDLFWAI GGGGSSFGVVVAYKIR VRV
Sbjct: 190 NMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAYKIRTVRV 249

Query: 249 PETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFL 308
           PETVTVFRV RTLE+N++TEIVD+WQRVAHVID DLFIRVTFDVVNG+  G KTLRATF+
Sbjct: 250 PETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKKTLRATFI 309

Query: 309 AMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALLNRMTGRI 368
           A+FLGDS+ LLS++N SFPKLGLKK DCLEMSW++SVLFWT+    TSV+ LL+R    +
Sbjct: 310 ALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELLSRTPQVL 369

Query: 369 -HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNL 428
            HLKRKSDYVKTLIPK+  N+IWKKMIELEKP M FNPYGGRM EI SNATPFPHRAGNL
Sbjct: 370 THLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPFPHRAGNL 429

Query: 429 WKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKS 488
           WKIQY TNW+E+G++QA+H IDLTRKL+KFMTPFV+KNPRTAFLNYRDLDIGV HNGK+S
Sbjct: 430 WKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGVNHNGKRS 489

Query: 489 YYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           YYEGR +GIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFP+V
Sbjct: 490 YYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYV 533

BLAST of CmoCh13G005050 vs. NCBI nr
Match: XP_022927445.1 (berberine bridge enzyme-like 8 [Cucurbita moschata])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.7e-309
Identity = 528/528 (100.00%), Postives = 528/528 (100.00%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60
           MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL
Sbjct: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60

Query: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120
           QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD
Sbjct: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120

Query: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180
           IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG
Sbjct: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180

Query: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240
           HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA
Sbjct: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240

Query: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300
           YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN
Sbjct: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300

Query: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR 360
           KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR
Sbjct: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR 360

Query: 361 MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420
           MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR
Sbjct: 361 MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420

Query: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480
           AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN
Sbjct: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480

Query: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV
Sbjct: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 528

BLAST of CmoCh13G005050 vs. NCBI nr
Match: KAG6583831.1 (Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.5e-304
Identity = 520/528 (98.48%), Postives = 522/528 (98.86%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60
           MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL
Sbjct: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60

Query: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120
           QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD
Sbjct: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120

Query: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180
           IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG
Sbjct: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180

Query: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240
           HL GGGYGNMMRKHGLTVDQ IDAKIIDVNGKLLDRK MGEDLFWAITGGGGSSFGVVVA
Sbjct: 181 HLGGGGYGNMMRKHGLTVDQIIDAKIIDVNGKLLDRKEMGEDLFWAITGGGGSSFGVVVA 240

Query: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300
           YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN
Sbjct: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300

Query: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNALLNR 360
           KTLRATFLAMFLGDS+TLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSV ALLNR
Sbjct: 301 KTLRATFLAMFLGDSKTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVKALLNR 360

Query: 361 MTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420
            TGRIHLKRKSDYVKTLIPKKG NKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR
Sbjct: 361 TTGRIHLKRKSDYVKTLIPKKGLNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHR 420

Query: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480
           AGNLWKIQYTTNWNEEGSRQADHLIDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIGVTHN
Sbjct: 421 AGNLWKIQYTTNWNEEGSRQADHLIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGVTHN 480

Query: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV
Sbjct: 481 GKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 528

BLAST of CmoCh13G005050 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 901.7 bits (2329), Expect = 2.9e-258
Identity = 440/532 (82.71%), Postives = 489/532 (91.92%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVL 60
           M+ L+SS IL+LSFS+L+ F+SA +A ENF QCLLR SPPSYPIAPAIFTPNN S+SSVL
Sbjct: 1   MEILRSS-ILLLSFSYLVFFSSATSAPENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVL 60

Query: 61  QTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSD 120
           +TYI+NLRFNTSTTPKPFLILTALHE HV++AILCARI +L MKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSD 120

Query: 121 IVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGG 180
           ++PFFILDMFNLR I++D+KT+TAWVQTGAT+GEV+Y+IAE++K +GFPAGVCPT+GVGG
Sbjct: 121 VLPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGG 180

Query: 181 HLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVA 240
           H  GGGYGNMMRK+GL+VDQ IDAKI+DVNGKLLDRKAMGEDLFWAI GGGGSSFGVVVA
Sbjct: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGN 300
           YKIR+VRVPETVTVFRV+RTLE+N+LTEIVDEWQRVAHVIDNDLFIRVTFDV+NGT KG 
Sbjct: 241 YKIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGK 300

Query: 301 KTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNAL 360
           KTLRATFLAMFLGDS+ LLS+I  SFPKLGLKK DCLEMSW++SVLFWT+    TSV+AL
Sbjct: 301 KTLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDAL 360

Query: 361 LNRMTGRI-HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATP 420
           L+R    + HLKRKSDYVKT IPK+G NKIWKKMIELEKPM+ FNPYGGRM EI SNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQY TNW+EEG+++A H IDLTRKL+KFMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 VTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           V HNGK SYYEGR YGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 531

BLAST of CmoCh13G005050 vs. NCBI nr
Match: XP_008445642.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuline oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 893.3 bits (2307), Expect = 1.0e-255
Identity = 441/533 (82.74%), Postives = 482/533 (90.43%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASA-KTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSV 60
           M+ L+SS IL LSFSFL+ F+SA  +A ENF+QCLLR SPPSYPIAPAIFTPNN SYSSV
Sbjct: 1   MQILRSS-ILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSV 60

Query: 61  LQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS 120
           L+TYI+NLRFNTSTTPKPFLILTALHE HV++AILCARI NL MKIRSGGHDYEGVSYVS
Sbjct: 61  LETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVS 120

Query: 121 DIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVG 180
           D+VPFF+LDMFNLR I++D KTETAWVQTGAT+GEV+Y++AE++K +GFPAGVCPT+GVG
Sbjct: 121 DVVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVG 180

Query: 181 GHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVV 240
           GH  GGGYGNMMRKHGL+VDQ IDAKIIDVNGKLLDRKAMGEDLFWAI GGGGSSFGVVV
Sbjct: 181 GHFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 240

Query: 241 AYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKG 300
           AYKIR+VRVPE VTVFRV+RTLE+NELTEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT K 
Sbjct: 241 AYKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKE 300

Query: 301 NKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNA 360
            KTLRATF+A+FLGDS  LL +INNSFPKLGLKK DC+EMSW++SVLFWT+    TSV A
Sbjct: 301 KKTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 360

Query: 361 LLNRMTGRI-HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNAT 420
           LL+R    + HLKRKSDYVK  IPK+G +KIWKKMIELEKPMM FNPYGGRM EI SNAT
Sbjct: 361 LLSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNAT 420

Query: 421 PFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDI 480
           PFPHRAGNLWKIQY TNW+EEG R+A H IDLTRKL+KFMTPFVSKNPRTAFLNYRDLDI
Sbjct: 421 PFPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 480

Query: 481 GVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GV HNGK SYYEGR YGIKYFEGNFERLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 GVNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CmoCh13G005050 vs. NCBI nr
Match: XP_004135723.1 (berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical protein Csa_023313 [Cucumis sativus])

HSP 1 Score: 889.8 bits (2298), Expect = 1.1e-254
Identity = 438/533 (82.18%), Postives = 484/533 (90.81%), Query Frame = 0

Query: 1   MKTLKSSVILVLSFSFLLVFASA-KTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSV 60
           M+ L++S IL+LSFSFL+ F+SA  +A ENF QCLLR SPPSYPIAPAIFTPNN SYSSV
Sbjct: 1   MEILRTS-ILLLSFSFLVFFSSASSSAPENFFQCLLRHSPPSYPIAPAIFTPNNASYSSV 60

Query: 61  LQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS 120
           L+TYI+NLRFNTSTTPKPFLILTALHE HV+ AILCARI NL MKIRSGGHDYEGVSYVS
Sbjct: 61  LETYIRNLRFNTSTTPKPFLILTALHESHVKAAILCARIHNLQMKIRSGGHDYEGVSYVS 120

Query: 121 DIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVG 180
           D+VPFFILDMFNLR I++D KT+TAWVQTGAT+GEV+Y++AE +K +GFPAGVCPT+GVG
Sbjct: 121 DVVPFFILDMFNLREITVDAKTKTAWVQTGATLGEVYYRVAENNKSYGFPAGVCPTVGVG 180

Query: 181 GHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVV 240
           GH  GGGYGNMMRK+GL+VDQ IDAKIIDVNGKLLDRKAMGEDLFWAI GGGGSSFGVVV
Sbjct: 181 GHFGGGGYGNMMRKYGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVV 240

Query: 241 AYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKG 300
           AYKIR+V VPETVTVFRV+RTLE+N+LTEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT+KG
Sbjct: 241 AYKIRMVPVPETVTVFRVQRTLEQNDLTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTNKG 300

Query: 301 NKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNA 360
            KTLRATF+A+FLGDS+ LL +INNSFPKLGLKK DC+EMSW++SVLFWT+    TSV A
Sbjct: 301 KKTLRATFIALFLGDSERLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEA 360

Query: 361 LLNRMTGRI-HLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNAT 420
           LL+R    + HLKRKSDYVKT IPK+G NKIWKKMIELEKPM+ FNPYGGRM EI SNAT
Sbjct: 361 LLSRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNAT 420

Query: 421 PFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDI 480
           PFPHRAGNLWKIQY TNW EEG+++A H IDLTRKL+KFMTPFVSKNPRTAFLNYRDLDI
Sbjct: 421 PFPHRAGNLWKIQYATNWVEEGNKEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDI 480

Query: 481 GVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHV 529
           GV HNGK SYYEGR YGIKYFEGNF+RLVKIKTKVDP NFFRNEQSIPRFPHV
Sbjct: 481 GVNHNGKNSYYEGRVYGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of CmoCh13G005050 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 657.9 bits (1696), Expect = 6.8e-189
Identity = 330/530 (62.26%), Postives = 404/530 (76.23%), Query Frame = 0

Query: 4   LKSSVILVLSF-SFLLVFASAKTATENFVQCLLRRSPPSYPIAPAIFTPNNHSYSSVLQT 63
           +K ++ILVL F  F+   +S+   +E F QCL   S P +PI+PAIF   N SYSSVLQ 
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQA 60

Query: 64  YIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVS-DI 123
            I+NLRFNT++TPKPFLI+ A HE HV+ AI C +  NL MKIRSGGHDY+G+SYV+   
Sbjct: 61  NIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 120

Query: 124 VPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGH 183
            PFF+LDMFNLR + +D+ ++TAWVQTGA +GEV+Y I E+SK   +PAG+CPT+GVGGH
Sbjct: 121 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 180

Query: 184 LSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAY 243
           +SGGGYGNMMRK+GLTVD  IDA+++DVNGK+LDRK MGEDL+WAI GGGG S+GVV+AY
Sbjct: 181 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 240

Query: 244 KIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNK 303
           KI LV VPE VTVFR+ RTLE+N  T+I+  WQ+VA  + ++LFIR   DVVNGT    K
Sbjct: 241 KINLVEVPENVTVFRISRTLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK 300

Query: 304 TLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALL 363
           T+R TF+AMFLGD+ TLLS++N  FP+LGL + DC E SWI+SVLFWT+    +S   LL
Sbjct: 301 TVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLL 360

Query: 364 NRMTGRIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFP 423
            R     +LKRKSDYV+  I + G   IWKKMIELE P M FNPYGG MG I+S  TPFP
Sbjct: 361 QRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFP 420

Query: 424 HRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGV- 483
           +RAGNLWKIQY  NW +E     D  ++LTRKL++FMTPFVSKNPR +F NYRD+D+G+ 
Sbjct: 421 YRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGIN 480

Query: 484 THNGK-KSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFP 527
           +HNGK  SY EG+ YG KYF GNFERLVKIKT+VD  NFFRNEQSIP  P
Sbjct: 481 SHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of CmoCh13G005050 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 554.7 bits (1428), Expect = 8.1e-158
Identity = 285/531 (53.67%), Postives = 375/531 (70.62%), Query Frame = 0

Query: 9   ILVLSFSFLLV----FASAKTAT-----ENFVQCLL-RRSPPSYPIAPAIFTPNNHSYSS 68
           + V  F F LV    F+SA   +     E+FVQC   +   P   I   +F+  N S+SS
Sbjct: 8   VSVFFFVFFLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSS 67

Query: 69  VLQTYIQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYV 128
           VL+ YI+N RFNTS+TPKP +I+T   + HV  A+ C++  N L+KIRSGGHDYEG+SY+
Sbjct: 68  VLRAYIRNGRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYI 127

Query: 129 SDIVPFFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGV 188
           SD  PFFILDM NLR +S+DI  ++AW+  GAT+GEV+Y+I E+SK+HGFPAGVCPT+GV
Sbjct: 128 SD-KPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGV 187

Query: 189 GGHLSGGGYGNMMRKHGLTVDQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVV 248
           GGH+SGGGYGNM+RK GL+VD  IDAKI+DVNG++LDRK+MGEDLFWAI+GGGG+SFGVV
Sbjct: 188 GGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVV 247

Query: 249 VAYKIRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHK 308
           + YK++LV VPETVTVFRV + ++   + ++V +WQ V    D +LF+R+    V  T K
Sbjct: 248 LGYKVKLVPVPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRK 307

Query: 309 GNKTLRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTDETSVNAL- 368
             KT+RAT +A+FLG ++ +++L+   FP+L LKK +C EM+W +S L+W +  +   + 
Sbjct: 308 KVKTVRATVVALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQID 367

Query: 369 ----LNRMTGRIHL-KRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIAS 428
               L+R   R +  KRKSDYV + IP+ G   ++KKM EL K  + FNPYGG+M E+  
Sbjct: 368 PKVFLDRNLDRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTV 427

Query: 429 NATPFPHRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRD 488
           NATPFPHR+  L+KIQY+  W E         ++    L+ FMT FVSKNPR A+LNYRD
Sbjct: 428 NATPFPHRS-KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRD 487

Query: 489 LDIGVTHNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
           +DIGV  +G  SY EG  YG KYF  NF+RLVK+KT  DPDNFFRNEQSIP
Sbjct: 488 VDIGVNDHGTNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of CmoCh13G005050 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 545.0 bits (1403), Expect = 6.4e-155
Identity = 273/511 (53.42%), Postives = 364/511 (71.23%), Query Frame = 0

Query: 20  FASAKTATENFVQCLL-RRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLRFNTSTTPKPF 79
           F+ + +   +F++C   +   P   I   +F+  N ++SSVL+ YI+N RFNTS+T KP 
Sbjct: 29  FSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNTSSTLKPT 88

Query: 80  LILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILDMFNLRRISID 139
           +I+T   E HV  A+ C++  N L+KIRSGGHDY+G+SY+SD  PFFILDM N+R +S+D
Sbjct: 89  IIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILDMSNIRDVSVD 148

Query: 140 IKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYGNMMRKHGLTV 199
           I + +AW+  GAT+GEV+Y+I E+S++HGFPAGVCPT+GVGGHLSGGGYGNM+RK GL+V
Sbjct: 149 IASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGNMVRKFGLSV 208

Query: 200 DQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRVPETVTVFRVR 259
           D   DAKI+DVNG++LDRKAMGEDLFWAITGGGG S+GVV+ YK++LV VP  VTVFRV 
Sbjct: 209 DYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVVTVFRVE 268

Query: 260 RTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFLAMFLGDSQTL 319
           + ++   + ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +A+FLG +  +
Sbjct: 269 QYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVVALFLGRADEV 328

Query: 320 LSLINNSFPKLGLKKPDCLEMSWIESVLFWTD-----ETSVNALLNRMTGRIHL-KRKSD 379
           ++L++  FP+LGLKK +C EM+W +S L+W +     +      L+R        KRKSD
Sbjct: 329 VALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKRKSD 388

Query: 380 YVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNLWKIQYTTN 439
           YV T IPKKG   ++KKMIEL K  + FNPYGG+M E+A NA PFPHR   L+KIQY+ N
Sbjct: 389 YVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQYSVN 448

Query: 440 WNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKSYYEGRGYG 499
           W E  +      ++  + L+ FMT FVSKNPR+++ NYRD+DIGV  +G  SY EG  YG
Sbjct: 449 WKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGEVYG 508

Query: 500 IKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
            KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 509 RKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CmoCh13G005050 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 545.0 bits (1403), Expect = 6.4e-155
Identity = 273/511 (53.42%), Postives = 364/511 (71.23%), Query Frame = 0

Query: 20  FASAKTATENFVQCLL-RRSPPSYPIAPAIFTPNNHSYSSVLQTYIQNLRFNTSTTPKPF 79
           F+ + +   +F++C   +   P   I   +F+  N ++SSVL+ YI+N RFNTS+T KP 
Sbjct: 29  FSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNTSSTLKPT 88

Query: 80  LILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVPFFILDMFNLRRISID 139
           +I+T   E HV  A+ C++  N L+KIRSGGHDY+G+SY+SD  PFFILDM N+R +S+D
Sbjct: 89  IIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFILDMSNIRDVSVD 148

Query: 140 IKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLSGGGYGNMMRKHGLTV 199
           I + +AW+  GAT+GEV+Y+I E+S++HGFPAGVCPT+GVGGHLSGGGYGNM+RK GL+V
Sbjct: 149 IASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGYGNMVRKFGLSV 208

Query: 200 DQFIDAKIIDVNGKLLDRKAMGEDLFWAITGGGGSSFGVVVAYKIRLVRVPETVTVFRVR 259
           D   DAKI+DVNG++LDRKAMGEDLFWAITGGGG S+GVV+ YK++LV VP  VTVFRV 
Sbjct: 209 DYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVVTVFRVE 268

Query: 260 RTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKTLRATFLAMFLGDSQTL 319
           + ++   + ++V +WQ V    D +LF+R+    V  T K  KT+RA+ +A+FLG +  +
Sbjct: 269 QYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASVVALFLGRADEV 328

Query: 320 LSLINNSFPKLGLKKPDCLEMSWIESVLFWTD-----ETSVNALLNRMTGRIHL-KRKSD 379
           ++L++  FP+LGLKK +C EM+W +S L+W +     +      L+R        KRKSD
Sbjct: 329 VALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKRKSD 388

Query: 380 YVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFPHRAGNLWKIQYTTN 439
           YV T IPKKG   ++KKMIEL K  + FNPYGG+M E+A NA PFPHR   L+KIQY+ N
Sbjct: 389 YVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR-NKLFKIQYSVN 448

Query: 440 WNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVTHNGKKSYYEGRGYG 499
           W E  +      ++  + L+ FMT FVSKNPR+++ NYRD+DIGV  +G  SY EG  YG
Sbjct: 449 WKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGEVYG 508

Query: 500 IKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
            KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 509 RKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of CmoCh13G005050 vs. TAIR 10
Match: AT1G30720.1 (FAD-binding Berberine family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.8e-149
Identity = 265/525 (50.48%), Postives = 371/525 (70.67%), Query Frame = 0

Query: 11  VLSFSFLLVFASAKTATEN------FVQCLLRR-SPPSYPIAPAIFTPNNHSYSSVLQTY 70
           +L  S LL+ +++ T +++      F++CL R+ + P+ P +   + P N S+++VL++ 
Sbjct: 4   LLVISLLLLISTSVTTSQSVTDPIAFLRCLDRQPTDPTSPNSAVAYIPTNSSFTTVLRSR 63

Query: 71  IQNLRFNTSTTPKPFLILTALHEYHVRIAILCARIDNLLMKIRSGGHDYEGVSYVSDIVP 130
           I NLRF+  TTPKP  ++ A    H++ A+ CAR  +L ++IRSGGHD+EG+SY S  VP
Sbjct: 64  IPNLRFDKPTTPKPISVVAAATWTHIQAAVGCARELSLQVRIRSGGHDFEGLSYTS-TVP 123

Query: 131 FFILDMFNLRRISIDIKTETAWVQTGATIGEVFYKIAEESKLHGFPAGVCPTMGVGGHLS 190
           FF+LDMF  + + +++   TAWV +GAT+GE++Y+I+E+S + GFPAG+  T+GVGGH S
Sbjct: 124 FFVLDMFGFKTVDVNLTERTAWVDSGATLGELYYRISEKSNVLGFPAGLSTTLGVGGHFS 183

Query: 191 GGGYGNMMRKHGLTVDQFIDAKIIDVNGKLL-DRKAMGEDLFWAITGGGGSSFGVVVAYK 250
           GGGYGN+MRK+GL+VD    + I+D NG +  DR +MGED FWAI GGG +S+GVV+ YK
Sbjct: 184 GGGYGNLMRKYGLSVDNVFGSGIVDSNGNIFTDRVSMGEDRFWAIRGGGAASYGVVLGYK 243

Query: 251 IRLVRVPETVTVFRVRRTLEENELTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTHKGNKT 310
           I+LV VPE VTVF+V +T+ E  + +++ +WQ  AH  D +LF+R+T  +VNGT  G  T
Sbjct: 244 IQLVPVPEKVTVFKVGKTVGEGAV-DLIMKWQSFAHSTDRNLFVRLTLTLVNGTKPGENT 303

Query: 311 LRATFLAMFLGDSQTLLSLINNSFPKLGLKKPDCLEMSWIESVLFWTD---ETSVNALLN 370
           + ATF+ M+LG S  LL+++N  FP+L LKK DC EM WI+SVLFW D    T  + LLN
Sbjct: 304 VLATFIGMYLGRSDKLLTVMNRDFPELKLKKTDCTEMRWIDSVLFWDDYPVGTPTSVLLN 363

Query: 371 RMTG-RIHLKRKSDYVKTLIPKKGFNKIWKKMIELEKPMMKFNPYGGRMGEIASNATPFP 430
            +   ++ +KRKSDYVK LI +     I KK++E+EK  M +NPYGGRMGEI S+ TPFP
Sbjct: 364 PLVAKKLFMKRKSDYVKRLISRTDLGLILKKLVEVEKVKMNWNPYGGRMGEIPSSRTPFP 423

Query: 431 HRAGNLWKIQYTTNWNEEGSRQADHLIDLTRKLHKFMTPFVSKNPRTAFLNYRDLDIGVT 490
           HRAGNL+ I+Y  +W+E G       + L  + ++FMTP+VS NPR AFLNYRDLDIG  
Sbjct: 424 HRAGNLFNIEYIIDWSEAGDNVEKKYLALANEFYRFMTPYVSSNPREAFLNYRDLDIG-- 483

Query: 491 HNGKKSYYEGRGYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIP 524
            + K +Y EG+ YG KYF+ NFERLV IKT +D +NF++NEQSIP
Sbjct: 484 SSVKSTYQEGKIYGAKYFKENFERLVDIKTTIDAENFWKNEQSIP 524

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SA859.6e-18862.26Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q9SVG31.1e-15653.67Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=... [more]
Q9SVG49.1e-15453.42Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=... [more]
Q9SA872.6e-14850.48Berberine bridge enzyme-like 10 OS=Arabidopsis thaliana OX=3702 GN=At1g30720 PE=... [more]
F4HV093.3e-14851.87Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1ENY71.8e-309100.00berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111434269 PE=... [more]
A0A5A7TVZ25.0e-25682.74Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BE215.0e-25682.74reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=... [more]
A0A6J1GSQ12.2e-25181.61berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=... [more]
A0A6J1K3U36.3e-25181.30berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_022927445.13.7e-309100.00berberine bridge enzyme-like 8 [Cucurbita moschata][more]
KAG6583831.13.5e-30498.48Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038895093.12.9e-25882.71berberine bridge enzyme-like 8 [Benincasa hispida][more]
XP_008445642.11.0e-25582.74PREDICTED: reticuline oxidase-like protein [Cucumis melo] >KAA0047124.1 reticuli... [more]
XP_004135723.11.1e-25482.18berberine bridge enzyme-like 8 [Cucumis sativus] >KAE8653304.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
AT1G30700.16.8e-18962.26FAD-binding Berberine family protein [more]
AT4G20840.18.1e-15853.67FAD-binding Berberine family protein [more]
AT4G20830.16.4e-15553.42FAD-binding Berberine family protein [more]
AT4G20830.26.4e-15553.42FAD-binding Berberine family protein [more]
AT1G30720.11.8e-14950.48FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 466..523
e-value: 1.5E-21
score: 76.2
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 81..214
e-value: 3.8E-19
score: 68.8
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 24..132
e-value: 8.1E-38
score: 130.5
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 136..523
e-value: 1.5E-157
score: 526.9
NoneNo IPR availableGENE3D3.40.462.20coord: 251..462
e-value: 1.5E-157
score: 526.9
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 19..524
NoneNo IPR availablePANTHERPTHR32448:SF25BERBERINE BRIDGE ENZYME-LIKE 8coord: 19..524
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 73..248
score: 13.397823
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 22..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G005050.1CmoCh13G005050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding