CmoCh12G001670 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh12G001670 vs. ExPASy Swiss-Prot
Match: B8N5T6 (Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglB PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 5.7e-25
Identity = 152/673 (22.59%), Postives = 270/673 (40.12%), Query Frame = 0

Query: 15  VTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLVWNPSASAT-GSFD- 74
           +T+ N  +  T +   G V+ +  +G D L  +  +     Y D    PS   T GS D 
Sbjct: 30  ITLANDRLTSTFAKDKGRVSELFLDGQDLLGPISGNTGVGPYLDCYCIPSGFYTAGSTDP 89

Query: 75  ---VIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPL--LIDKRFIMLRGSSGFYSYA 134
              V++ TD             + T+   + L     P      + + +  G +G ++++
Sbjct: 90  RLEVVQGTD------------STGTKYAGVILNDTYTPTGQQFQQYWFLRDGETGLHTFS 149

Query: 135 IYQHLKEWPGF--NLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPE 194
              +  E   F  NL E R  F+   + + ++  ++   ++ PLP   +      +   +
Sbjct: 150 RLAYYNETTPFLRNLQEFRTLFRPNTELWTHLTSSE--AQTAPLPSKEA--IANEVVVQD 209

Query: 195 AVLLVDPIESELK----GEVDDKYQYSCDNKDSGVHGWI-----STDPPIGFWLITPSNE 254
           A    +   ++       E   KY +S   +D+ VHG       S     G WL+  + +
Sbjct: 210 ATWRFNNTPNDAYYTQFSEYFTKYTFSNLWRDNSVHGLYADGTNSNGTTYGAWLVMNTKD 269

Query: 255 CRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDS 314
              GGP+  +LT  V        +S H+ GE   P I  G  + + FGP +   N    S
Sbjct: 270 TYYGGPLHSDLT--VDGIVYNYLVSNHH-GEG-TPNITNG--FDRTFGPQYYLFNGGKGS 329

Query: 315 SDALQLWEDGKSQA-SIEVQSWPYSFPAS-----EDFPKSNQRGNISGRL-LVKDSYVSE 374
             +L   ED +S+A ++    W   F  S       +  S++RG++ G++ L K S    
Sbjct: 330 KSSL---EDLRSEAETLADPGWNADFYDSIAKHVIGYAPSSKRGSVQGQVKLPKGSTRPI 389

Query: 375 DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFI 434
             +  +G +              E+  +Q+W+   +DG F +DH++ G Y L  +  G  
Sbjct: 390 AILTVDGQY--------FQDNSVEASSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIF 449

Query: 435 GDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPD-PNPNYVNKL 494
           GD+ +D  +++  G    V E  +E   AG  +W +G PD+S+ EF   D P+P +    
Sbjct: 450 GDFVHDG-VEVQAGKVTKVQE-TWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTHP--- 509

Query: 495 YLNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKD-----WFFAQVTRKTENGTFKG 554
            L+    F  +G ++ + + +P+  + YT+G SD   D     W     T    +  +  
Sbjct: 510 -LHPPQHFIYWGAYD-WQQDFPNG-VNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDT 569

Query: 555 T-TWQVKFELD--NVDSQATYKLRVALASASIA----------------ELQVGVNDPKA 614
           T  W + F LD   +  + T  L + LA A  A                 L+  +N+ K 
Sbjct: 570 THDWTINFSLDKKQLQQRKTATLTIQLAGAKTAAGNTDVYNATEPYANLALESYINEQKE 629

Query: 615 KPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQA---- 630
                 G     + I R  +        +  P + L  G+N +    PR+ + ++     
Sbjct: 630 PLTLLVGFNQSSSCIVRSAVSCYQVRSRMEFPADWLNVGNNVLTLHLPRNATDYETAVLP 661

BLAST of CmoCh12G001670 vs. ExPASy Swiss-Prot
Match: Q5B5P1 (Probable rhamnogalacturonate lyase C OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglC PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.9e-13
Identity = 122/548 (22.26%), Postives = 204/548 (37.23%), Query Frame = 0

Query: 16  TMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRG-YWDLVWNPSASATGSFD-- 75
           T+ N    V ++  +G V     +G D LL  L+  S +G Y D    P    T   +  
Sbjct: 33  TISNSRFSVAVAKSNGHVVDANLDGQD-LLGPLSGNSGKGPYLDCSCTPEGFWTPGAEPA 92

Query: 76  VIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRG-SSGFYSYAIYQH 135
           ++  TD          V +  T  ++ + +          +++ LRG  +G ++++   +
Sbjct: 93  LVNGTD----STGTPYVGVIMTDTYETTNQTL-------SQYLFLRGEETGLHAFSRVTY 152

Query: 136 LKEWPGF--NLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLL 195
             E   F   LGE R  F+   + + + + ++     MPL          A  Y     L
Sbjct: 153 YNESDYFLRGLGELRTLFRPNTNLWTHFSGSEGNYGPMPLSSTEKITVQDATTY-----L 212

Query: 196 VDPIESELKGEVDD---KYQYSCDNKDSGVHGWISTDP------PIGFWLITPSNECRSG 255
            D  +     +  D   KY  +   +D  VHG  S           G WL+  + E   G
Sbjct: 213 GDTTDDPYVSQYSDYFTKYTLTESWRDHDVHGHFSNGSTSGDGNTYGAWLVHNTRETYYG 272

Query: 256 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 315
           GP+  +L   V        +S HY      P       + + FGP + + NS    +   
Sbjct: 273 GPLHADLV--VDGIVYNYIVSGHYG----APNPNLTHGFDRTFGPQYYHFNSGGPGTTLE 332

Query: 316 QLWEDGKSQASIEVQSWPYSFPASE--DFPKSNQRGNISGRLLVKDSYVSEDYIPANGAF 375
           +L  D    AS E  +  Y   A    ++  S  R    G++ +         + +    
Sbjct: 333 ELRADAAQYASPEWNAEFYDSIAKHIPNYVPSTGRTTFRGKVNLPKGAKKPIIVLSEN-- 392

Query: 376 VGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSII 435
                   +  ++++S   Q+W+  D  G F I  +  G Y +  +     G +  D++ 
Sbjct: 393 ---EQDFQLNVFKKDS--LQYWAEIDGSGAFTIPRVVKGTYRVTIYADEIFGWFIKDNVK 452

Query: 436 DILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEF---YVPDPNPNYVNKLYLNHSDK 495
            I  GS  +     ++   AG  +W IGVPD+S+ EF   Y PD +            ++
Sbjct: 453 VI--GS--NAHTFTWKEETAGKEIWRIGVPDKSSGEFLHGYAPDTSKPL-------QPEQ 512

Query: 496 FRQYGLWERYSELYPD---ADLVYTVGVSDYTKD-----W-FFAQVTRKTENGTF--KGT 533
           +R Y  W +Y   YP      + Y VG SD  KD     W FF        N  +     
Sbjct: 513 YRIY--WGKYD--YPSDFPEGVNYHVGKSDPAKDLNYIHWSFFPSQGNHLRNEPYYQNVN 535

BLAST of CmoCh12G001670 vs. ExPASy TrEMBL
Match: A0A6J1GGG5 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 SV=1)

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60
           MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60

Query: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS 120
           WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS
Sbjct: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240
           LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG
Sbjct: 181 LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL
Sbjct: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300

Query: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360
           QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG
Sbjct: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI 420
           LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI 420

Query: 421 LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG 480
           LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG
Sbjct: 421 LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG 480

Query: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540
           LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT
Sbjct: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540

Query: 541 YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600
           YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL
Sbjct: 541 YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600

Query: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL 642
           VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL
Sbjct: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL 641

BLAST of CmoCh12G001670 vs. ExPASy TrEMBL
Match: A0A6J1GGK0 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 641/652 (98.31%), Postives = 641/652 (98.31%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRG----- 60
           MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRG     
Sbjct: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYIMNE 60

Query: 61  ------YWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLL 120
                 YWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLL
Sbjct: 61  KLASYKYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLL 120

Query: 121 IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL 180
           IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL
Sbjct: 121 IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL 180

Query: 181 PDDRSPPRGRALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFW 240
           PDDRSPPRGRALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFW
Sbjct: 181 PDDRSPPRGRALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFW 240

Query: 241 LITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY 300
           LITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY
Sbjct: 241 LITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY 300

Query: 301 LNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE 360
           LNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE
Sbjct: 301 LNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE 360

Query: 361 DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFI 420
           DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFI
Sbjct: 361 DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFI 420

Query: 421 GDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLY 480
           GDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLY
Sbjct: 421 GDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLY 480

Query: 481 LNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK 540
           LNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK
Sbjct: 481 LNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK 540

Query: 541 FELDNVDSQATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYW 600
           FELDNVDSQATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYW
Sbjct: 541 FELDNVDSQATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYW 600

Query: 601 LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL 642
           LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL
Sbjct: 601 LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGLL 652

BLAST of CmoCh12G001670 vs. ExPASy TrEMBL
Match: A0A6J1KMB3 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV=1)

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 627/640 (97.97%), Postives = 634/640 (99.06%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60
           MPPLGVQLSI+EHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV
Sbjct: 1   MPPLGVQLSISEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60

Query: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS 120
           WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPL IDKRFIMLRGS
Sbjct: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFIMLRGS 120

Query: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA
Sbjct: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180

Query: 181 LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240
           LAYPEAVLLVDPIESEL+GEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG
Sbjct: 181 LAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300
           GPVKQNLTSHVGPT+LAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYD SDA 
Sbjct: 241 GPVKQNLTSHVGPTSLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDFSDAF 300

Query: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360
           QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG
Sbjct: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360

Query: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI 420
           LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDH+RPGDYNLYAWVPGFIGDYRYDSII+I
Sbjct: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRPGDYNLYAWVPGFIGDYRYDSIINI 420

Query: 421 LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG 480
           +EGSQIDVDEIIYEPPRAGATLWEIGVPDRSA EFYVPDPNPNYVNKLYLNH DKFRQYG
Sbjct: 421 IEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYG 480

Query: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540
           LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT
Sbjct: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540

Query: 541 YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600
           YKLRVALASASI ELQVGVNDPKAKP+FSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL
Sbjct: 541 YKLRVALASASIVELQVGVNDPKAKPLFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600

Query: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 641
           VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL
Sbjct: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 640

BLAST of CmoCh12G001670 vs. ExPASy TrEMBL
Match: A0A6J1KHT0 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV=1)

HSP 1 Score: 1308.1 bits (3384), Expect = 0.0e+00
Identity = 627/651 (96.31%), Postives = 634/651 (97.39%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRG----- 60
           MPPLGVQLSI+EHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRG     
Sbjct: 1   MPPLGVQLSISEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYIMNE 60

Query: 61  ------YWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLL 120
                 YWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPL 
Sbjct: 61  KLASYKYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLE 120

Query: 121 IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL 180
           IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL
Sbjct: 121 IDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPL 180

Query: 181 PDDRSPPRGRALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFW 240
           PDDRSPPRGRALAYPEAVLLVDPIESEL+GEVDDKYQYSCDNKDSGVHGWISTDPPIGFW
Sbjct: 181 PDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGVHGWISTDPPIGFW 240

Query: 241 LITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY 300
           LITPSNECRSGGPVKQNLTSHVGPT+LAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY
Sbjct: 241 LITPSNECRSGGPVKQNLTSHVGPTSLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIY 300

Query: 301 LNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE 360
           LNSTYD SDA QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE
Sbjct: 301 LNSTYDFSDAFQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSE 360

Query: 361 DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFI 420
           DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDH+RPGDYNLYAWVPGFI
Sbjct: 361 DYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRPGDYNLYAWVPGFI 420

Query: 421 GDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLY 480
           GDYRYDSII+I+EGSQIDVDEIIYEPPRAGATLWEIGVPDRSA EFYVPDPNPNYVNKLY
Sbjct: 421 GDYRYDSIINIIEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFYVPDPNPNYVNKLY 480

Query: 481 LNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK 540
           LNH DKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK
Sbjct: 481 LNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVK 540

Query: 541 FELDNVDSQATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYW 600
           FELDNVDSQATYKLRVALASASI ELQVGVNDPKAKP+FSSGLIGRDNSIARHGIHGLYW
Sbjct: 541 FELDNVDSQATYKLRVALASASIVELQVGVNDPKAKPLFSSGLIGRDNSIARHGIHGLYW 600

Query: 601 LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 641
           LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL
Sbjct: 601 LYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 651

BLAST of CmoCh12G001670 vs. ExPASy TrEMBL
Match: A0A6J1BSU2 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111005034 PE=3 SV=1)

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 573/640 (89.53%), Postives = 604/640 (94.38%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLV 60
           MPPLGVQL + E QV MDNGIVQVT SNPDGIV GIRYNGVDNLLE+LNDE NRGYWDLV
Sbjct: 76  MPPLGVQLYVTERQVVMDNGIVQVTFSNPDGIVIGIRYNGVDNLLEILNDEYNRGYWDLV 135

Query: 61  WNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGS 120
           W+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRKWDLSLEGKLVPL IDKRF+MLRGS
Sbjct: 136 WSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGS 195

Query: 121 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 180
           SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA
Sbjct: 196 SGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRA 255

Query: 181 LAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSG 240
           LAYPEAVLLVDP+E EL+GEVDDKYQYSCDNKDS VHGWISTDPP+GFWLITPSNE RSG
Sbjct: 256 LAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSG 315

Query: 241 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAL 300
           GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDA+
Sbjct: 316 GPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAI 375

Query: 301 QLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVG 360
           QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI GRLLVKD YVSED+IPANGAFVG
Sbjct: 376 QLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVG 435

Query: 361 LAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDI 420
           LAPPGDVGSWQRESKGYQFWSRADE GYF I+ +R GDYNLYAWVPGFIGDYR DS+I+I
Sbjct: 436 LAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINI 495

Query: 421 LEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYG 480
            EGS+IDV +IIYE PR GAT+WEIG+PDRSA EFYVPDPNP YVNKLYLNH DKFRQYG
Sbjct: 496 SEGSEIDVGDIIYEAPREGATMWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYG 555

Query: 481 LWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT 540
           LWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKTENGTFKGTTWQVKF+LDNVD + T
Sbjct: 556 LWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGT 615

Query: 541 YKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRL 600
           YKLR+ALASAS+AELQV VNDPK+KP FSSGLIGRDNSIARHGIHGL+WLYNV+IP NRL
Sbjct: 616 YKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRL 675

Query: 601 VSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSSAHGL 641
           V+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSSAH L
Sbjct: 676 VAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSSAHAL 715

BLAST of CmoCh12G001670 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 975.7 bits (2521), Expect = 1.8e-284
Identity = 447/617 (72.45%), Postives = 523/617 (84.76%), Query Frame = 0

Query: 17  MDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKA 76
           MDNGI +VTLS PDGIVTGI YNG+DNLLEVLN+E NRGYWDLVW  S +A G FDVIK 
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGSGTA-GGFDVIKG 60

Query: 77  TDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGSSGFYSYAIYQHLKEWP 136
           ++FEVIV+NE+Q+ELSFTRKWD S EGK VPL IDKRF+ML GSSGFY+YAIY+HLKEWP
Sbjct: 61  SNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWP 120

Query: 137 GFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESE 196
            F+L ETR+AFKLRK+KFHYMA+ D+RQR MPLPDDR P RG+ALAYPEAVLLV+P+ES+
Sbjct: 121 AFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQ 180

Query: 197 LKGEVDDKYQYSCDNKDSGVHGWISTD-PPIGFWLITPSNECRSGGPVKQNLTSHVGPTT 256
            KGEVDDKYQYSC+NKD  VHGWI T+ P +GFWLITPS+E R+GGP KQNLTSHVGPT 
Sbjct: 181 FKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTA 240

Query: 257 LAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLN-STYDSSDALQLWEDGKSQASIEV 316
           LAVFISAHYTGEDLVPK   GEAWKKVFGPVF+YLN ST D +D L LW+D KSQ ++E 
Sbjct: 241 LAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEA 300

Query: 317 QSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRES 376
           +SWPYSFPAS+D+ K+ QRGN+ GRLLV+D YV +D+I AN  +VGLA PG  GSWQRE 
Sbjct: 301 ESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQREC 360

Query: 377 KGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDILEGSQIDVDEIIYE 436
           K YQFW+R DE+G+F+I  IRPG YNLYAW+PGFIGDY+YD +I I  G  I V++++Y+
Sbjct: 361 KEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQ 420

Query: 437 PPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYGLWERYSELYPDADL 496
           PPR GATLWEIG PDRSA EFYVPDPNP Y+N LY NH D+FRQYGLWERY+ELYPD DL
Sbjct: 421 PPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDL 480

Query: 497 VYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIAE 556
           VY VG SDY KDWF+AQVTRK +N T++GTTWQ+KFEL N+D   +Y LRVA+ASA+ +E
Sbjct: 481 VYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSE 540

Query: 557 LQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRS 616
           LQ+ VN+  A P+F+SGLIGRDNSIARHGIHGLYWL+NV + G++L+ G+NT+F TQPRS
Sbjct: 541 LQIRVNNANASPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRS 600

Query: 617 TSPFQAIMYDYIRLEAP 632
           TSPFQ IMYDYIR EAP
Sbjct: 601 TSPFQGIMYDYIRFEAP 616

BLAST of CmoCh12G001670 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 894.0 bits (2309), Expect = 6.9e-260
Identity = 411/629 (65.34%), Postives = 495/629 (78.70%), Query Frame = 0

Query: 5   GVQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLVWNPS 64
           GV L + +  V MDNGI+QVTLS P GI+TGI YNG+DN+LEV N E+NRGYWDL WN  
Sbjct: 48  GVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLHWN-E 107

Query: 65  ASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGSSGFY 124
               G FDVI    F VIVE E+QVE+SF R WD SLEGK +PL IDKRFIMLRGSSG Y
Sbjct: 108 PGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSSGVY 167

Query: 125 SYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYP 184
           SY IY+HLK+WPGF LGETR+AFKLRKDKFHYMA+AD+R+R MP PDD    R + L Y 
Sbjct: 168 SYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQ 227

Query: 185 EAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSGGPVK 244
           EA LL  P +  L+GEVDDKYQYSC+NKD  VHGWIS DPP+GFW ITPSNE RSGGP+K
Sbjct: 228 EASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLK 287

Query: 245 QNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDALQLWE 304
           QNLTSHVGPTTLAVF S HY G+ ++P+   GE WKKV+GPVFIYLNST +  D L LW+
Sbjct: 288 QNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWD 347

Query: 305 DGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLAPP 364
           D K +   EV+ WPYSF AS+D+PKS +RG   GRLL++D +++ D I A GA+VGLAPP
Sbjct: 348 DAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPP 407

Query: 365 GDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDILEGS 424
           GD GSWQ E KGYQFW+ ADE GYF I ++RPG+YNLYAWVP FIGDY   +I+ +  G 
Sbjct: 408 GDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGC 467

Query: 425 QIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYGLWER 484
            I++ +I+YEPPR G TLWEIG+PDR A EF++PDP+P  VN++ ++H D+FRQYGLW++
Sbjct: 468 MIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGLWKK 527

Query: 485 YSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQATYKLR 544
           Y+++YP+ DLVYTVGVSDY +DWFFA V RK +    +GTTWQ+ F L+N+D +A YKLR
Sbjct: 528 YTDMYPNDDLVYTVGVSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANYKLR 587

Query: 545 VALASASIAELQVGVNDPKA-KPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSG 604
           VA+ASA++AELQ+ +ND +A +P+F++GLIGRDNSIARHGIHG+Y LY VNIPGNRLV G
Sbjct: 588 VAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQG 647

Query: 605 DNTIFFTQPRSTSPFQAIMYDYIRLEAPP 633
           DNTIF  QPR   PFQ IMYDYIRLE PP
Sbjct: 648 DNTIFLKQPRCNGPFQGIMYDYIRLEGPP 674

BLAST of CmoCh12G001670 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 882.9 bits (2280), Expect = 1.6e-256
Identity = 411/640 (64.22%), Postives = 504/640 (78.75%), Query Frame = 0

Query: 6   VQLSIAEHQVTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDLVWNPSA 65
           VQL + E  V M NG V+VT+S PDG VTGI Y GVDNLLE  N++ NRGYWDLVW+   
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  S--ATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGSSGF 125
           +   TG  + IK T FEV+VENE+ VE+SF+RKWD SL+  + P+ +DKRFIM +  +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAY 185
           YSYAI++HL EWP FNL +TR+ +KLRKDKF YMA+ADNRQR MPLP+DR   RGR LAY
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSGGPV 245
           PEAVLLV P+E E KGEVDDKY+YS +NKD  VHGWIS +  +G W I PSNE RSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYD-SSDALQL 305
           KQNLTSHVGP +LA+F+SAHY GED+V K+ AG++WKKVFGPVF YLN   D +SD L L
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLA 365
           W+D K+Q   EVQSWPY FPASEDFP S++RG ISGRLLV D ++S+D++PANGAFVGLA
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 PPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDILE 425
           PPG+VGSWQ ESKGYQFW+ AD DGYF I+ IR G+YNL  +V G+IGDY+Y+ +I+I  
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYGLW 485
           G  IDV  I+YEPPR G T+WEIG+PDRSA EF+VPDPNP Y+NKLY+ H D+FRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 ERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQATYK 545
           ERY+ELYP  DLV+T+GVSDY KDWFFA VTRK  + T++ TTWQ+KF+L+NV    TYK
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYK 546

Query: 546 LRVALASASIAELQVGVND---PKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNR 605
           +R+ALA+A++AELQV +ND    K  P+F++G+IG DN+IARHGIHG+Y LYNV++P  +
Sbjct: 547 IRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEK 606

Query: 606 LVSGDNTIFFTQPRSTS-PFQAIMYDYIRLEAPPPPSSAH 639
           LV GDNT+F TQ  +T+  F  +MYDYIRLE PP  S +H
Sbjct: 607 LVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPPLDSYSH 646

BLAST of CmoCh12G001670 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 839.7 bits (2168), Expect = 1.5e-243
Identity = 393/623 (63.08%), Postives = 486/623 (78.01%), Query Frame = 0

Query: 17  MDNGIVQVTLSNPDGIVTGIRYNGVDNLLE-VLNDESNRGYWDLVWN----PSASATGSF 76
           M+N  +Q+TLSNP+G VTGI+YNG+DN+L    N E +RGYWD+VWN     +    G+ 
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 77  DVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRGSSGFYSYAIYQH 136
           D I+AT  EVI +N++++ELSFTR W+ S     VP+ IDKRF+ML+ SSGFYSYAI++ 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTS-STTAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 137 LKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVD 196
           L+ WP   L   RL FKL K KFHYMA++D+RQR MP+PDDR PPRG+ LAYPEAV L+D
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 197 PIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHV 256
           PIE E KGEVDDKY+YS ++KD  VHGWIST+  +GFW ITPSNE RS GP+KQ L SHV
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHV 240

Query: 257 GPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDALQLWEDGKSQAS 316
           GPT LAVF S HY G DL+     GEAWKKVFGPVFIYLNS     D L LW + K+Q  
Sbjct: 241 GPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTK 300

Query: 317 IEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQ 376
           IE + WPY+F AS+DFP S+QRG++SGRLLV+D ++S + IPANG++VGLA PGDVGSWQ
Sbjct: 301 IEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQ 360

Query: 377 RESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIIDILEGSQIDVDEI 436
           RE KGYQFWS+ADE+G F I+++R G YNLYA+ PGFIGDY  D++ DI  GS+I + ++
Sbjct: 361 RECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDL 420

Query: 437 IYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNHSDKFRQYGLWERYSELYPD 496
           +YEPPR G+TLWEIGVPDRSA EFY+PDPNP++VNKLYLNHSDK+RQYGLWERYSELYPD
Sbjct: 421 VYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPD 480

Query: 497 ADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQAT--YKLRVALAS 556
            D+VY V + DY+K+WFF QVTRK  NG +KGTTWQ++F+ D+     T  +KLR+ALA+
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 557 ASIAELQVGVNDPKA-KPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSGDNTIF 616
           +++AELQV VND  A  P+F +  IGRDN+IARHGIHGLYWLY+VN+P   L  G+NTI+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 617 FTQPRSTSPFQAIMYDYIRLEAP 632
            TQ  +TSPFQ +MYDYIRLE P
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYP 622

BLAST of CmoCh12G001670 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 764.2 bits (1972), Expect = 8.2e-221
Identity = 357/633 (56.40%), Postives = 461/633 (72.83%), Query Frame = 0

Query: 1   MPPLGVQLSIAEHQ-VTMDNGIVQVTLSNPDGIVTGIRYNGVDNLLEVLNDESNRGYWDL 60
           +P L VQL    H  V +DNGIVQVT SNP+G++TGI+Y+G+DN+L+   D+  RGYWD+
Sbjct: 47  IPALTVQLRRVGHDTVVVDNGIVQVTFSNPEGLITGIKYHGIDNVLDDKIDD--RGYWDV 106

Query: 61  VWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRKWDLSLEGKLVPLLIDKRFIMLRG 120
           VW        + D ++ T FE+I +NE+Q+E+SFTR W +S  G LVPL +DKR+I+  G
Sbjct: 107 VWYEPEKKQKT-DKLEGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSG 166

Query: 121 SSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGR 180
            SG Y Y I + L+ WP  ++ + R+ FKL   KF +MA++D+RQRSMP   DR     +
Sbjct: 167 VSGLYMYGILERLEGWPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADRE--NSK 226

Query: 181 ALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGVHGWISTDPPIGFWLITPSNECRS 240
           +LAY EAVLL +P     KGEVDDKY YS ++KD+ VHGWIS+DPP+GFW+ITPS+E R 
Sbjct: 227 SLAYKEAVLLTNPSNPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRL 286

Query: 241 GGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDA 300
           GGP+KQ+LTSH GP TL++F S HY G+++      GE WKKVFGPV  YLNS       
Sbjct: 287 GGPIKQDLTSHAGPITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDST 346

Query: 301 LQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFV 360
           L+LW D K Q + EV+SWPY F  SED+P  +QRG + G+ L+KDSYVS   I    AFV
Sbjct: 347 LRLWRDAKRQMAAEVKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFV 406

Query: 361 GLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRPGDYNLYAWVPGFIGDYRYDSIID 420
           GLAP G+ GSWQ ESKGYQFW++AD  G F I+++R G+Y+LYAW  GFIGDY+Y+  I 
Sbjct: 407 GLAPIGEAGSWQTESKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNIT 466

Query: 421 ILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFYVPDPNPNYVNKLYLNH-SDKFRQ 480
           I  GS+++V  ++YEPPR G TLWEIGVPDR+A EFY+PDP P  +NKLY+N   D+FRQ
Sbjct: 467 ITPGSEMNVGPLVYEPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQ 526

Query: 481 YGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQ 540
           YGLW+RY++LYP  DLVYT+GVSDY  DWFFA V R   N T++ TTWQ+ F L NV+  
Sbjct: 527 YGLWDRYADLYPQNDLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRI 586

Query: 541 ATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRDNSIARHGIHGLYWLYNVNIPGN 600
             Y LR+ALASA+ +ELQ+ +NDPK+  +F++G IG+DN+IARHGIHGLY LY++++ GN
Sbjct: 587 GRYTLRIALASAADSELQIRINDPKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGN 646

Query: 601 RLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAP 632
            L  GDNTIF TQ RS +PFQ IMYDYIRLE+P
Sbjct: 647 LLSVGDNTIFLTQTRSRTPFQGIMYDYIRLESP 674