CmoCh10G009010 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh10G009010
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	RING-type E3 ubiquitin transferase
Location	Cmo_Chr10: 4425223 .. 4427012 (+)
RNA-Seq Expression	CmoCh10G009010
Synteny	CmoCh10G009010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCGAAATCGAGGTTCCTTCCGACTTTCTGTGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAACTGCCCGGTGACAAAGCAGCCACTGATCGGTACAGATTTGACGCCGAACCATACGCTGCGTCGGGTGATTCAAGGGTGGTGCTGTGTCAACGCCGAGCACGGGGCGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGGTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGCAAGCATTCATACTATTTAATTTTCATTTTTCTTATTTTTTTATTAAAAAAATCTTATTTTTCTCCGATAATATTTAAAGGATTAGGGTTAGATCCACGGGATTGGAGAGGAAAACGGGATTAGGGTTAGATCCATGGGATTGGAGAAAAAAACGAAACATCGTTGTGCAAACTTTTTAAAATCGTGAGGCTGACGATGATACATAACGTGCTAAAGTGAACAATATTTGTTAGCTGTGAGCTTGAGTTGTTATAGTTAGCCCGATTTCAAAAATAAAAACAACTCTGAATTTAAAAAAAAACTTATTTTTCGATCATATATTTTAATTATATTTTATTTTTGAAATATGACGGGTAAATATTTTATTTTATTAAATAAAAGTTTATATAATTTTATTAAACTAATTTATTGAGAATAATAATTAAGATTTATTACTAGTGGTATAACTCGGCTCGTGCTATTGTCTATCGCGTTTGAGCTGCAAATGGTCTCTGTCTTCTCGTACGTTTAACACACAATTTTTAATTAACTAAAACATCTACTATCGATTCGGTAATGGAACATTATGATCGACAATATGTTTAGGATGCTCGATCCATGGAGTTCTTAGCATCAATGTTGAACAATCCCGAGGATGGGAATGAGGATGTCATTGTAGAAAAAGCTATAGAAATCATTTCGAACATCAACTCACCCACGACTCTCCTCGAGCATCTCTTAATCCGAAACTCTAACCTCATCGATGTCGTAATCTCCATCGTTGCCGTGACCAAAACCTCGAGATCCCGAGCGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAACCTTCACAAAAGACGATCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCACCCTCAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGTTGGCAATTATGGCCATGGACCAGCTTTGTGAATGTGCTGAAGGTCGGGCTGAGCTGCTCCGACACGGAGGAGGGATGGCCGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAGATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAGACTAATGAAAGAATCAAGGAAATTCTTCATTTTCTTCGATGCATTTTCAAGGGTACGAAGTGTGTTCTCGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

mRNA sequence

ATGCCCGAAATCGAGGTTCCTTCCGACTTTCTGTGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAACTGCCCGGTGACAAAGCAGCCACTGATCGGTACAGATTTGACGCCGAACCATACGCTGCGTCGGGTGATTCAAGGGTGGTGCTGTGTCAACGCCGAGCACGGGGCGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGGTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGATGCTCGATCCATGGAGTTCTTAGCATCAATGTTGAACAATCCCGAGGATGGGAATGAGGATGTCATTGTAGAAAAAGCTATAGAAATCATTTCGAACATCAACTCACCCACGACTCTCCTCGAGCATCTCTTAATCCGAAACTCTAACCTCATCGATGTCGTAATCTCCATCGTTGCCGTGACCAAAACCTCGAGATCCCGAGCGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAACCTTCACAAAAGACGATCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCACCCTCAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGTTGGCAATTATGGCCATGGACCAGCTTTGTGAATGTGCTGAAGGTCGGGCTGAGCTGCTCCGACACGGAGGAGGGATGGCCGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAGATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAGACTAATGAAAGAATCAAGGAAATTCTTCATTTTCTTCGATGCATTTTCAAGGGTACGAAGTGTGTTCTCGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

Coding sequence (CDS)

ATGCCCGAAATCGAGGTTCCTTCCGACTTTCTGTGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAACTGCCCGGTGACAAAGCAGCCACTGATCGGTACAGATTTGACGCCGAACCATACGCTGCGTCGGGTGATTCAAGGGTGGTGCTGTGTCAACGCCGAGCACGGGGCGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGGTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGATGCTCGATCCATGGAGTTCTTAGCATCAATGTTGAACAATCCCGAGGATGGGAATGAGGATGTCATTGTAGAAAAAGCTATAGAAATCATTTCGAACATCAACTCACCCACGACTCTCCTCGAGCATCTCTTAATCCGAAACTCTAACCTCATCGATGTCGTAATCTCCATCGTTGCCGTGACCAAAACCTCGAGATCCCGAGCGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAACCTTCACAAAAGACGATCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCACCCTCAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGTTGGCAATTATGGCCATGGACCAGCTTTGTGAATGTGCTGAAGGTCGGGCTGAGCTGCTCCGACACGGAGGAGGGATGGCCGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAGATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAGACTAATGAAAGAATCAAGGAAATTCTTCATTTTCTTCGATGCATTTTCAAGGGTACGAAGTGTGTTCTCGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

Protein sequence

MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF

Homology

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BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.5e-73
Identity = 158/402 (39.30%), Postives = 252/402 (62.69%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK--NCPVTKQPLIGTDLTP 62
           EIE+P  FLCPISL+IM DPV +STGITYDR+SIEKWL   K  +CPVTKQ +   DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ WC +NA +G ER+ +P+       + K+I +++     ++++CL +L   
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI-RDSASSHENQVKCLKRLRQI 128

Query: 123 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 182
           ++E   NK  L+ A   EFLA++++N  D     + ++A+ ++ ++ +  T+L++LL  N
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLL-NN 188

Query: 183 SNLIDVVISIVAVTKTS--RSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQI 242
               ++V S+  + +     SR  A   L ++  ++D       K ++F ++ + L+D+I
Sbjct: 189 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 243 ---AIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDQL 302
              A K  + IL+++ P+GRNR KAV+ GV+  ++ELL++ +   E    E+A++ +D L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 303 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNF----GVAEEM 362
           C+CAEGRAE L HG  +AVV +KILRVS  ++++AVR+L      +VG F     +  EM
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLL-----SVGRFCATPALLHEM 368

Query: 363 VEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCV 391
           +++G V KLCL+LQ+    KT E+ KE+L     ++K + C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

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BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 6.0e-73
Identity = 168/429 (39.16%), Postives = 255/429 (59.44%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK--NCPVTKQPLIGTDLTP 62
           EIE+PS FLCPISL IM DPV +STGITYDRESIEKWL   K  +CPVTKQ +  TDLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ WC +NA +G ER+ +PK       + K+I KE+     ++++CL +L   
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KESSSSHLNQVKCLKRLRQI 123

Query: 123 LAETERNKIYLQDARSMEFLASMLNNPED---------------------------GNED 182
           ++E   NK  L+ A   EFLA++++N  D                            +  
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 183 VIVEKAIEIISNINSPTTLLEHLL--IRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLY 242
            ++++A+ ++ ++++  T L+ LL   + +NL+  +  I+       SRA A   L  L 
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAALLLKKLL 243

Query: 243 SISDQTHKTFTKDDLFIQLSRALNDQIAIKPT---LKILLHLAPFGRNRIKAVKHGVVFD 302
            ++D       + +LF ++ + L+DQI+ K T   ++IL+   P+GRNR KAV+ G +  
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 303

Query: 303 LVELLLNSTVG---RECELAIMAMDQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNE 362
           ++ELL++ T     R  E+A++ +D LC+CAEGRAE L HG  +AVV++KILRVS  ++E
Sbjct: 304 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 363

Query: 363 KAVRILYHVCKNNVGNF----GVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 391
           +AVR+L      +VG F     + +EM+++G V KLCL+LQ+    KT E+ KE+L    
Sbjct: 364 RAVRVLL-----SVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 423

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BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.5e-47
Identity = 143/424 (33.73%), Postives = 222/424 (52.36%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPL-IGTDLTPN 62
           EIE+P+ F+CPISL+IM DPVT  +GITYDR++I KWL    +CPVTKQPL + +DLTPN
Sbjct: 7   EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPSCPVTKQPLPLDSDLTPN 66

Query: 63  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVI--LKEAMRGRSSRLECLNKLEA 122
           H LRR+IQ WC  N   G  R+ +P+      +VV+ I  LK+  +    R E L KLE 
Sbjct: 67  HMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEV 126

Query: 123 FLAETERNKIYLQ---DARSMEFLASMLNNPEDGNEDVI-VEKAIEIISNINSPTTLLEH 182
              +    ++  +       + F+    +  EDG   +  +++++ ++  I  P+   + 
Sbjct: 127 LAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKT 186

Query: 183 LLIRNSNLID----------------VVISIVAVTKTSRSR----------AAAIAFLSS 242
           +L+ N  +++                 ++ +  +T+ + S              I FL  
Sbjct: 187 ILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIGFLKD 246

Query: 243 LYSISDQTHKTF-------TKDDLF----IQLSRALNDQIAIKPTLKILLHLAPFGRNRI 302
           + +  ++T  T        ++  L      +L  +L  + A+   L ILL  + + RNR 
Sbjct: 247 VVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWSRNRS 306

Query: 303 KAVKHGVVFDLVELLLNST-VGRECELAIMAMDQLCECAEGRAELLRHGGGMAVVARKIL 362
             V  G V +L+EL ++ T   R  EL +  + +LC CA GRAE+L H GG+AVV +++L
Sbjct: 307 LLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLL 366

Query: 363 RVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEIL 380
           RVS  ++++A+ IL  V K +  N  V EEMV VG V KLC +L +   L   E+ KEIL
Sbjct: 367 RVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNLKEKAKEIL 426

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BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.6e-33
Identity = 109/406 (26.85%), Postives = 195/406 (48.03%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWL-LFCKNCPVTKQPLIGTDLTPN 62
           EI +PS F CPIS ++M DPV I++GITYDRE+IEKW     + CPVT   L   +  PN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 63  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGR----SSRLECLNKL 122
           HT+RR+IQGWC  +   G ER+ +P+       V ++  + +   R    ++ +E + K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 123 EAFLAETERN-KIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEI-------------- 182
                E+ERN K   ++   +          E+ N  +++E+ + +              
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWMLPIGLEGQSK 209

Query: 183 ISNINSPTTLLEHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFT 242
           ++  +S   L+E L   + N   ++  ++ +  T       +  L+ +  + +   K+  
Sbjct: 210 LTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTH------VHALTKINGVQEAFMKSIN 269

Query: 243 KDDLFIQLSRALNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE- 302
           +D      S  +N  I+I   +              + V   +  DLV + +   V  E 
Sbjct: 270 RD------STCVNSLISIHHMI----------LTNQETVSRFLELDLVNITVEMLVDSEN 329

Query: 303 --CELAIMAMDQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNV 362
             CE A+  ++ +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ VCK+  
Sbjct: 330 SVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKIS--EKKDLVSVMWKVCKS-- 389

Query: 363 GNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFK 386
           G+    EE + +GA  KL +MLQ+G    T E++ E+L  +  + K
Sbjct: 390 GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

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BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.6e-33
Identity = 113/392 (28.83%), Postives = 185/392 (47.19%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN--CPVTKQPLIGTDLTPN 63
           I++P  F CPISL++M DPVT+ TG TYDR SIE W+    N  CPVT+ PL    L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 64  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMR------GRSSRLECLN 123
           HTLRR+IQ WC  N  +G ER+ +PK   D    V+ +L +A           SR   L 
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTHVSVRSRAAALR 131

Query: 124 KLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEH 183
           +L  F  ++++N++ +    + E L  +L +    +E V    A+ ++  I  P   +  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS- 191

Query: 184 LLIRNSNLIDVVISIV--AVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRA 243
            +  +   ++ +  ++  +  +T  + AA I  +S+    +D        + +F  +   
Sbjct: 192 -ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDL 251

Query: 244 LNDQI----AIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAM 303
           L + I    A+K  +K L  L      R  A+  G    L++ L       + E A+  +
Sbjct: 252 LRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATV 311

Query: 304 DQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMV 363
           + LC   EG A    H   + ++ + ILRVS+ + E A   L  +C          EE  
Sbjct: 312 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWREEAA 371

Query: 364 EVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 382
             G V +L LM+Q   + +  ++ +++L  LR
Sbjct: 372 GAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

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BLAST of CmoCh10G009010 vs. ExPASy TrEMBL
Match: A0A6J1HD28 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732 PE=4 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 3.8e-224
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0


Query: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60
           MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60

Query: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 180
           LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN
Sbjct: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 180

Query: 181 SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240
           SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI
Sbjct: 181 SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240

Query: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300
           KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA
Sbjct: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmoCh10G009010 vs. ExPASy TrEMBL
Match: A0A6J1JH48 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 PE=4 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.2e-212
Identity = 381/400 (95.25%), Postives = 386/400 (96.50%), Query Frame = 0


Query: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60
           M EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK CPVTKQPLIG DLTP
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60

Query: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGW CVN EHG ERVRSPKGEGDGGHVVK+ILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 180
           LAETERNKIYLQDARSMEFL SMLNNPED NE+VIVEKAIEII NINSPTTLLEHLLI+N
Sbjct: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180

Query: 181 SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240
           +NLID VISIVAVTKTSRSR AAIAFLSSLYSISDQTHK FTKD+LFIQLSRALNDQIAI
Sbjct: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240

Query: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300
           KP LKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD LCECAEGRA
Sbjct: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmoCh10G009010 vs. ExPASy TrEMBL
Match: A0A0A0LXW1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE=4 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.3e-159
Identity = 294/407 (72.24%), Postives = 341/407 (83.78%), Query Frame = 0


Query: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN----CPVTKQPLIGT 60
           M EIEVPSDFLCPISLQ+M DPVTISTGITYDRESI+KWL  CKN    CPV+KQ L   
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRG-RSSRLECLN 120
           DLTPNHTLRRVIQGWC +N  HG E++ +P+ E D G VVK+ILKEAM+  RSS LECL 
Sbjct: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120

Query: 121 KLEAFLAETERNKIYLQDARSMEFLASML--NNPEDGNEDVIVEKAIEIISNINSPTTLL 180
           +L++ +AE E NKIYLQ+  SM FL S+L  +  ED +ED +VE+AIEII++INSPT +L
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180

Query: 181 EHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRA 240
            ++L +N  LID +ISI+  TK+ +SRA+ IAFLSSLY+ISDQ HKTFTKD LFIQL+RA
Sbjct: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240

Query: 241 LNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLC 300
           L DQ+A KP L+ILL LAPFGRNRIKAVKHGVVF +VELLLNST G+ECELA+  +D+LC
Sbjct: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300

Query: 301 ECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGA 360
           ECAEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG 
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           VGKLCLMLQ+GG+LKT ERIKEILH L+C+FKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 406

[Close]

BLAST of CmoCh10G009010 vs. ExPASy TrEMBL
Match: A0A1S3BNV9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.8e-156
Identity = 289/405 (71.36%), Postives = 336/405 (82.96%), Query Frame = 0


Query: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN----CPVTKQPLIGT 60
           M EIEVPSDFLCPISLQIM DPVTISTGITYDRE I+KWL  CKN    CPV+KQ L   
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLICPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRG-RSSRLECLN 120
           DLTPNHTL RVIQGWC ++  HG E++ +PK E D G  VK ILKEAM+  RSSRLECL 
Sbjct: 61  DLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLECLK 120

Query: 121 KLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEH 180
           +L++ +AE E NKIYLQ+  SM FL S+L   E  +EDV+VE+AIEII+NINSP T+L++
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEGEDEDVMVEEAIEIITNINSPATILKN 180

Query: 181 LLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALN 240
           LL +N  LID +ISI+  TK+ +SRA+ IAFL SLY+ISDQTHKTFTKD LFIQL+RAL 
Sbjct: 181 LLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTRALM 240

Query: 241 DQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCEC 300
           DQI+ KP L+ILL LAPFGRNRIKA+K+GVVF +VELLLNS+ GRECELA+  +D+LCEC
Sbjct: 241 DQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRLCEC 300

Query: 301 AEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVG 360
           AEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG V 
Sbjct: 301 AEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVA 360

Query: 361 KLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           KLCLMLQ+GG+LKT ER+KEILH L+C+ +GTKCV V+PSGFDRF
Sbjct: 361 KLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCV-VVPSGFDRF 404

[Close]

BLAST of CmoCh10G009010 vs. ExPASy TrEMBL
Match: A0A6J1EH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 3.4e-148
Identity = 277/403 (68.73%), Postives = 327/403 (81.14%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN-------CPVTKQPLIGT 63
           +EVPSDFLCPISLQIM DPVTISTGITYDRESIEKWL   KN       CPVTK PL   
Sbjct: 3   VEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSYKNRHVVVPSCPVTKLPLSEL 62

Query: 64  DLTPNHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNK 123
           DLTPNHTLRR+IQGWC +NA  G E + +PKGE D G VVK++ KEAMR  SSRLECL +
Sbjct: 63  DLTPNHTLRRLIQGWCSLNAGDGVEPIPTPKGEVDRGQVVKLV-KEAMRCGSSRLECLKR 122

Query: 124 LEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHL 183
           L+A + E ERNK YLQ+  SMEFL S+LNN    N++ ++E+AIEI+SN NSPTTLL+HL
Sbjct: 123 LKAIVVENERNKFYLQEVESMEFLTSVLNN----NDESMIEEAIEILSNTNSPTTLLKHL 182

Query: 184 LIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALND 243
           L +N NLI  +ISI+ +TK+S S AAAIAFLSSLY+ISDQTHKTFT+D LFI L+RAL+D
Sbjct: 183 LTQNPNLIQTLISIITITKSSISSAAAIAFLSSLYTISDQTHKTFTQDTLFIHLTRALSD 242

Query: 244 QIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECA 303
           QIA  P+L+ILLHLAP GRNR KAVKHG VF L++LL  S++ RECELA+  +D+LCECA
Sbjct: 243 QIATNPSLQILLHLAPHGRNRFKAVKHGAVFHLIQLLFGSSIPRECELAMATLDRLCECA 302

Query: 304 EGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGK 363
           EGR ELLRHGGG+AVVARKILRVSN +NEKAVRILY+VCKNNVGNF V EEMVEVGAVGK
Sbjct: 303 EGRVELLRHGGGVAVVARKILRVSNLTNEKAVRILYNVCKNNVGNFRVFEEMVEVGAVGK 362

Query: 364 LCLMLQIGGSLKTNERIKEILHFLRCIFKG-TKCVLVLPSGFD 399
           LC ++++GGSLK  ER+K ILH ++  F G +KCV+V+P  FD
Sbjct: 363 LCAVIEVGGSLKIIERVKVILHLIQRAFDGSSKCVIVIPPRFD 400

[Close]

BLAST of CmoCh10G009010 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 276.9 bits (707), Expect = 2.5e-74
Identity = 158/402 (39.30%), Postives = 252/402 (62.69%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK--NCPVTKQPLIGTDLTP 62
           EIE+P  FLCPISL+IM DPV +STGITYDR+SIEKWL   K  +CPVTKQ +   DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ WC +NA +G ER+ +P+       + K+I +++     ++++CL +L   
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI-RDSASSHENQVKCLKRLRQI 128

Query: 123 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 182
           ++E   NK  L+ A   EFLA++++N  D     + ++A+ ++ ++ +  T+L++LL  N
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLL-NN 188

Query: 183 SNLIDVVISIVAVTKTS--RSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQI 242
               ++V S+  + +     SR  A   L ++  ++D       K ++F ++ + L+D+I
Sbjct: 189 KKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 243 ---AIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDQL 302
              A K  + IL+++ P+GRNR KAV+ GV+  ++ELL++ +   E    E+A++ +D L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 303 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNF----GVAEEM 362
           C+CAEGRAE L HG  +AVV +KILRVS  ++++AVR+L      +VG F     +  EM
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLL-----SVGRFCATPALLHEM 368

Query: 363 VEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCV 391
           +++G V KLCL+LQ+    KT E+ KE+L     ++K + C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

[Close]

BLAST of CmoCh10G009010 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 276.2 bits (705), Expect = 4.2e-74
Identity = 168/429 (39.16%), Postives = 255/429 (59.44%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK--NCPVTKQPLIGTDLTP 62
           EIE+PS FLCPISL IM DPV +STGITYDRESIEKWL   K  +CPVTKQ +  TDLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ WC +NA +G ER+ +PK       + K+I KE+     ++++CL +L   
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KESSSSHLNQVKCLKRLRQI 123

Query: 123 LAETERNKIYLQDARSMEFLASMLNNPED---------------------------GNED 182
           ++E   NK  L+ A   EFLA++++N  D                            +  
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 183 VIVEKAIEIISNINSPTTLLEHLL--IRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLY 242
            ++++A+ ++ ++++  T L+ LL   + +NL+  +  I+       SRA A   L  L 
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAALLLKKLL 243

Query: 243 SISDQTHKTFTKDDLFIQLSRALNDQIAIKPT---LKILLHLAPFGRNRIKAVKHGVVFD 302
            ++D       + +LF ++ + L+DQI+ K T   ++IL+   P+GRNR KAV+ G +  
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 303

Query: 303 LVELLLNSTVG---RECELAIMAMDQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNE 362
           ++ELL++ T     R  E+A++ +D LC+CAEGRAE L HG  +AVV++KILRVS  ++E
Sbjct: 304 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 363

Query: 363 KAVRILYHVCKNNVGNF----GVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 391
           +AVR+L      +VG F     + +EM+++G V KLCL+LQ+    KT E+ KE+L    
Sbjct: 364 RAVRVLL-----SVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 423

[Close]

BLAST of CmoCh10G009010 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 191.0 bits (484), Expect = 1.8e-48
Identity = 143/424 (33.73%), Postives = 222/424 (52.36%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPL-IGTDLTPN 62
           EIE+P+ F+CPISL+IM DPVT  +GITYDR++I KWL    +CPVTKQPL + +DLTPN
Sbjct: 21  EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPSCPVTKQPLPLDSDLTPN 80

Query: 63  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVI--LKEAMRGRSSRLECLNKLEA 122
           H LRR+IQ WC  N   G  R+ +P+      +VV+ I  LK+  +    R E L KLE 
Sbjct: 81  HMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEV 140

Query: 123 FLAETERNKIYLQ---DARSMEFLASMLNNPEDGNEDVI-VEKAIEIISNINSPTTLLEH 182
              +    ++  +       + F+    +  EDG   +  +++++ ++  I  P+   + 
Sbjct: 141 LAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKT 200

Query: 183 LLIRNSNLID----------------VVISIVAVTKTSRSR----------AAAIAFLSS 242
           +L+ N  +++                 ++ +  +T+ + S              I FL  
Sbjct: 201 ILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIGFLKD 260

Query: 243 LYSISDQTHKTF-------TKDDLF----IQLSRALNDQIAIKPTLKILLHLAPFGRNRI 302
           + +  ++T  T        ++  L      +L  +L  + A+   L ILL  + + RNR 
Sbjct: 261 VVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWSRNRS 320

Query: 303 KAVKHGVVFDLVELLLNST-VGRECELAIMAMDQLCECAEGRAELLRHGGGMAVVARKIL 362
             V  G V +L+EL ++ T   R  EL +  + +LC CA GRAE+L H GG+AVV +++L
Sbjct: 321 LLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRLL 380

Query: 363 RVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEIL 380
           RVS  ++++A+ IL  V K +  N  V EEMV VG V KLC +L +   L   E+ KEIL
Sbjct: 381 RVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNLKEKAKEIL 440

[Close]

BLAST of CmoCh10G009010 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-34
Identity = 109/406 (26.85%), Postives = 195/406 (48.03%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWL-LFCKNCPVTKQPLIGTDLTPN 62
           EI +PS F CPIS ++M DPV I++GITYDRE+IEKW     + CPVT   L   +  PN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 63  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGR----SSRLECLNKL 122
           HT+RR+IQGWC  +   G ER+ +P+       V ++  + +   R    ++ +E + K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 123 EAFLAETERN-KIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEI-------------- 182
                E+ERN K   ++   +          E+ N  +++E+ + +              
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVLTWMLPIGLEGQSK 209

Query: 183 ISNINSPTTLLEHLLIRNSNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFT 242
           ++  +S   L+E L   + N   ++  ++ +  T       +  L+ +  + +   K+  
Sbjct: 210 LTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTH------VHALTKINGVQEAFMKSIN 269

Query: 243 KDDLFIQLSRALNDQIAIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE- 302
           +D      S  +N  I+I   +              + V   +  DLV + +   V  E 
Sbjct: 270 RD------STCVNSLISIHHMI----------LTNQETVSRFLELDLVNITVEMLVDSEN 329

Query: 303 --CELAIMAMDQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNV 362
             CE A+  ++ +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ VCK+  
Sbjct: 330 SVCEKALTVLNVICETKEGREKVRRNKLVIPILVKKILKIS--EKKDLVSVMWKVCKS-- 389

Query: 363 GNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFK 386
           G+    EE + +GA  KL +MLQ+G    T E++ E+L  +  + K
Sbjct: 390 GDGSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

[Close]

BLAST of CmoCh10G009010 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-34
Identity = 113/392 (28.83%), Postives = 185/392 (47.19%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKN--CPVTKQPLIGTDLTPN 63
           I++P  F CPISL++M DPVT+ TG TYDR SIE W+    N  CPVT+ PL    L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 64  HTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMR------GRSSRLECLN 123
           HTLRR+IQ WC  N  +G ER+ +PK   D    V+ +L +A           SR   L 
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTHVSVRSRAAALR 131

Query: 124 KLEAFLAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEH 183
           +L  F  ++++N++ +    + E L  +L +    +E V    A+ ++  I  P   +  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS- 191

Query: 184 LLIRNSNLIDVVISIV--AVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRA 243
            +  +   ++ +  ++  +  +T  + AA I  +S+    +D        + +F  +   
Sbjct: 192 -ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDL 251

Query: 244 LNDQI----AIKPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAM 303
           L + I    A+K  +K L  L      R  A+  G    L++ L       + E A+  +
Sbjct: 252 LRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATV 311

Query: 304 DQLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMV 363
           + LC   EG A    H   + ++ + ILRVS+ + E A   L  +C          EE  
Sbjct: 312 ELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWREEAA 371

Query: 364 EVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 382
             G V +L LM+Q   + +  ++ +++L  LR
Sbjct: 372 GAGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

The following BLAST results are available for this feature:

BLAST of CmoCh10G009010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	3.5e-73	39.30	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	6.0e-73	39.16	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	2.5e-47	33.73	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9C8D1	4.6e-33	26.85	U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1...	[more]
Q9LT79	4.6e-33	28.83	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]

BLAST of CmoCh10G009010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HD28	3.8e-224	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732...	[more]
A0A6J1JH48	1.2e-212	95.25	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 P...	[more]
A0A0A0LXW1	3.3e-159	72.24	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE...	[more]
A0A1S3BNV9	5.8e-156	71.36	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 ...	[more]
A0A6J1EH89	3.4e-148	68.73	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282...	[more]

BLAST of CmoCh10G009010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	2.5e-74	39.30	plant U-box 23	[more]
AT3G52450.1	4.2e-74	39.16	plant U-box 22	[more]
AT3G11840.1	1.8e-48	33.73	plant U-box 24	[more]
AT1G66160.1	3.3e-34	26.85	CYS, MET, PRO, and GLY protein 1	[more]
AT3G19380.1	3.3e-34	28.83	plant U-box 25	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 9..71 e-value: 7.2E-30 score: 115.2
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 6..75 e-value: 4.4E-19 score: 68.4
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 5..78 score: 37.45446
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..85 e-value: 3.4E-28 score: 99.5
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 100..382 e-value: 2.2E-12 score: 48.2
None	No IPR available	PANTHER	PTHR22849:SF11	OS11G0573500 PROTEIN	coord: 5..392
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 5..392
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 10..51 e-value: 5.23354E-21 score: 83.437
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 4..76
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 110..362

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh10G009010.1	CmoCh10G009010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity