CmoCh08G009820 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh08G009820
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCmo_Chr08: 6392551 .. 6393639 (-)
RNA-Seq ExpressionCmoCh08G009820
SyntenyCmoCh08G009820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAATTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGCGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCAGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTGACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

mRNA sequence

ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAATTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGCGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCAGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTGACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

Coding sequence (CDS)

ATGGAATCCATCGGAATCTCGGATCCCTCTCCGCCGTTCAATCCGTTCAACTTCAAGGACTGTTCTCAACAAATTTGCACCGCCTTCTGTCCGCAGTGGTGCGCCTTCTTGTTCCCGCCGCCTCCGCCGTTCTTCGGCCTCGACGATAGCGACGATTCCAGTACGTTCTTCTCGCCACTTATAGTCGCAGTGATCGGGATCTTGGCGAGCGCGTTTGTTCTCGTGACTTACTACGCGATCGTCTCGAAATATTGCCGACGGAGTGTTGGCGACGGCGGAGGCGGAGGACCGGAGGAGAGTTTCGAGACGAATCGAGCGATTAACGAAACGCGGAGGTCTAGTGCGGGATCTGGATTGGATGAGGCGCTGATTAAATCGATCTCGGTTTACAAGTTCAAGAAGGGCGAAGGATTGATCGAAGGAAGCGATTGCTCTGTTTGTTTGAGTGAATTTCAAGAGAATGAGAGTTTGCGATTGTTGCCTAAATGTAGTCACGCTTTTCACCTCCCCTGTATTGATACTTGGCTGAAATCCAATTCGAGTTGCCCTCTCTGTCGCAGCAATATCGCTTCAACGAATCCTCCTCCTCAGGAGACTCCACCACCGCCCGTGACGGCGCTAGGTTACCGCCACAGCAGCGACGCCGCTGTGGTCGTCGTCCAAGATTTATCCGCAAACGTAAGCCAGGAATCCGTCGTAGTTGAAATCGTAAGCGATTCAACTAATCAAGAACAAGAAAATCCGAATCCGAGTCCAAATCCTAGAAGTTTCGATCAAGGAGACGGAAGAACAGAGAGAAGAGAAGAATCCGTCCATTCGATTAGATCAGTTTCAGAAGATCATCACTCCTCTCCATGTCCAGGCGAGAATCTCTCTGTTGCGGATGTATTGAACATCAACCACGAAGAAGACGAAGAAATTGAAGCTCTGGATTCATCCATGGCAGCCGGAATCGGTCCCTCAAACGGCAACAACAAAGAAACAGAAAAATCCAGAAACAAAAACGGCGTACTGAGAATGTCGATTTCATCTGGAATTTTCTCGTTCACAAGGTATACAAAAGGGAAGAACTCGAATTCGCCAAATTAA

Protein sequence

MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNSPN
Homology
BLAST of CmoCh08G009820 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.1e-60
Identity = 148/368 (40.22%), Postives = 214/368 (58.15%), Query Frame = 0

Query: 12  PFNPF-NFKDCSQQICTAFCPQWCAFLFPPPPPFFGL-DDSDDSSTFFSPLIVAVIGILA 71
           P++P+ ++ DCSQ IC  +CPQWC  +FPPPPP F L DDS  SS+ FSPL++A+IGIL 
Sbjct: 7   PWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILT 66

Query: 72  SAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRR-SSAGSGLDEALIKSIS 131
           SA +LV+YY ++SKYC R            +       + T+R S+ G GL+E++IKSI+
Sbjct: 67  SALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSIT 126

Query: 132 VYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSN 191
           VYK+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKS+S+CPLCR+ 
Sbjct: 127 VYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAF 186

Query: 192 IASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQ--------ESVVV--EIVS 251
           +   N P           +  + +S      V +++ N++         +SVVV  ++  
Sbjct: 187 VTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEI 246

Query: 252 DSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLN 311
            S N +  +    P  R       R  + E+S+   RSVS +       G  +S+ADVL 
Sbjct: 247 GSRNSDARSELQLPEER-------RETKDEDSLPIRRSVSLN------SGVVVSIADVLR 306

Query: 312 INHEEDEEIEALDSSMAA-----GIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYT 362
              +E+ E   + +S        G G +    +  ++S   +G    S+S+G F F+RY 
Sbjct: 307 EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGRFIFSRYD 361

BLAST of CmoCh08G009820 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 223.0 bits (567), Expect = 5.4e-57
Identity = 141/369 (38.21%), Postives = 203/369 (55.01%), Query Frame = 0

Query: 17  NFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVT 76
           +++DCSQ +C+ +CPQWC  +FPPPP F+  D+ D SS+ FSPL++A+IGILASAF+LV+
Sbjct: 16  SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVS 75

Query: 77  YYAIVSKYCRRSVGDG------------------GGGGPEESFETNRAINETRRSSAGSG 136
           YY ++SKYC R   +                   G      +  TN   N+T     G G
Sbjct: 76  YYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNP--NQTIGGGGGDG 135

Query: 137 LDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKS 196
           LDE+LIKSI+VYK++K +G +E SDCSVCLSEFQENESLRLLPKC+HAFH+PCIDTWLKS
Sbjct: 136 LDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 195

Query: 197 NSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVV---E 256
           +S+CPLCR+ I +++          V  +   +      +V ++ S +   +SVVV   +
Sbjct: 196 HSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENNSISTGDDSVVVVNLD 255

Query: 257 IVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVAD 316
           + +  +  E  N   +P P          ERR  S++S              G  +S+AD
Sbjct: 256 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNS-------------GGGVVSIAD 315

Query: 317 VLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNK-NGVLRMSISSGIFSFTR--Y 362
           +L    E+DEE   + +S     G        +  + N+ NG+    + S + +  R  Y
Sbjct: 316 ILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMKRSGY 355

BLAST of CmoCh08G009820 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 203.4 bits (516), Expect = 4.4e-51
Identity = 139/361 (38.50%), Postives = 192/361 (53.19%), Query Frame = 0

Query: 6   ISDPSPPFNPFN-FKDCSQQICTAFCPQWCAFLFPPPPPF--FGLDDSDDSSTFFSPLIV 65
           ++  +P + P+   +DCSQ +C+ +CPQWC F+FPPPPPF   G    D S   FSPL++
Sbjct: 1   MASSAPAWVPYEPTRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVI 60

Query: 66  AVIGILASAFVLVTYYAIVSKYC----------------RRSVGDGGGGGPEESFETNRA 125
           A+IG+LASAF+LV+YY  +SKYC                  + G G G G       +R+
Sbjct: 61  AIIGVLASAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRHGHGQSRS 120

Query: 126 INETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHA 185
            +E+   S  SGLDE LI  I+V K+++G+G +  +DCSVCL EF + ESLRLLP+CSHA
Sbjct: 121 -HESWNVSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHA 180

Query: 186 FHLPCIDTWLKSNSSCPLCRSNIASTN----PPPQETPPPPVTALGYRHSSDAAVVVVQD 245
           FH  CIDTWLKS+S+CPLCR+NI         P  E   P  T     H     VVVV D
Sbjct: 181 FHQQCIDTWLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHE----VVVVMD 240

Query: 246 LSANVSQESVVVEIVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSE-DH 305
              N+ +E    E VS ++  + ++ +        ++ +G  E REE     R  S  D 
Sbjct: 241 GLENLCEEQ--QEAVSRASTADDDH-DAKDVAEGMEEANGAAEIREEGSPPKRGASSFDL 300

Query: 306 HSSPCPGENLSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMS 343
           H        + +ADVL     EDE   A +S + AG G         E S+ + G  R +
Sbjct: 301 HRD----NRMCIADVLQ-ESMEDELTAARESGLLAG-GAGTSRRCHGENSKGRGGRSRRA 347

BLAST of CmoCh08G009820 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 7.1e-41
Identity = 92/204 (45.10%), Postives = 128/204 (62.75%), Query Frame = 0

Query: 7   SDPSP-PFNPF-NFKDCSQQICTAFCPQWCAFL-FPPPPPFFG--LDDSDDSSTFFSPLI 66
           SDP+P  +N + N +DC+Q +C+ FCPQWC ++ F PPP  +   L+D   S+   SPL+
Sbjct: 3   SDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLV 62

Query: 67  VAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRA-----INETRR---- 126
           +A+ GI A+AF+L  YY +VSKYC     +      E + E+ R+     +N   R    
Sbjct: 63  IAIFGIFATAFLLAAYYTLVSKYCANDTTN------EAASESGRSDIILDVNSPERGDQD 122

Query: 127 -----SSAGSGLDEALIKSISVYKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSH 186
                 S+ +GLD+ LIK I  +K KK +    I G+DCS+CL EF E+ESLRLLPKC+H
Sbjct: 123 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 182

Query: 187 AFHLPCIDTWLKSNSSCPLCRSNI 190
            FH+ CID WLKS+S+CPLCR+ I
Sbjct: 183 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of CmoCh08G009820 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 80/226 (35.40%), Postives = 124/226 (54.87%), Query Frame = 0

Query: 3   SIGISDPSPPFNPF---NFKDCSQQICTAFCPQWCAFLFPPPPP-----FFGLDDSDDSS 62
           S G+ DP  P+N +   ++   S Q+     P W +   P P P        L   D SS
Sbjct: 24  SNGVCDPICPYNCYPEPDYYTISPQL-----PPWSSSPQPSPCPSPSISAVYLPSQDSSS 83

Query: 63  TFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGG-----------PEESFET 122
           +  +  I+ + G + +  +L+T + +V+K+   SV     G             EE F+ 
Sbjct: 84  SLDAISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQD 143

Query: 123 NRAINETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKC 182
              ++        +GL +++I SI++  +K+G+GLIE +DC VCL+EF+E+ESLRLLPKC
Sbjct: 144 REQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKC 203

Query: 183 SHAFHLPCIDTWLKSNSSCPLCRSNIASTN-PPPQETPPPPVTALG 209
           +HAFH+ CIDTWL S+++CPLCR+ IA  +   P+ + P  VT  G
Sbjct: 204 NHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGG 242

BLAST of CmoCh08G009820 vs. ExPASy TrEMBL
Match: A0A6J1HMW7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 8.9e-204
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of CmoCh08G009820 vs. ExPASy TrEMBL
Match: A0A6J1KJE3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 2.8e-197
Identity = 353/362 (97.51%), Postives = 356/362 (98.34%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSV DGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQGDGRTERREES+HSIRSVSEDHHSSPCPGENLSVADVL+IN
Sbjct: 241 TNQEQEN----PNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKN NS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNPNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of CmoCh08G009820 vs. ExPASy TrEMBL
Match: A0A6J1H2X8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.8e-156
Identity = 290/366 (79.23%), Postives = 317/366 (86.61%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ++CTAFCPQWCA  F PPPP FGLDDSDDS TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQELCTAFCPQWCALPF-PPPPLFGLDDSDDSDTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R +N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVVNDTRQSSAGPGLN 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EAL+KSISV+KFKKGEGLIE SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALLKSISVFKFKKGEGLIEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240

Query: 241 TNQEQ--ENPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVL 300
           TN++Q   NPNP+PNP S D+GDGRTER REES+ SIRSVSEDH SSPCP   LSVAD+L
Sbjct: 241 TNRDQGSPNPNPNPNPPSVDRGDGRTERNREESIRSIRSVSEDHQSSPCPVRILSVADIL 300

Query: 301 NINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYT 360
           +IN EEDEE+EA++SSMAAG GPSN + KETEKSRN+N VL    RMSI SGIFSF RY 
Sbjct: 301 SINEEEDEEVEAMNSSMAAGSGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGIFSFQRYV 360

BLAST of CmoCh08G009820 vs. ExPASy TrEMBL
Match: A0A6J1L482 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.2e-154
Identity = 284/364 (78.02%), Postives = 314/364 (86.26%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFNF DCSQ++CTAFCPQWCAF F PPPP FGLDDSD S TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNFNDCSQKLCTAFCPQWCAFPF-PPPPLFGLDDSDKSDTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V DGGG  P E+FE +R  N+TR+SSAG GL+
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDDGGGAEPVENFEADRVANDTRQSSAGPGLN 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISV+KFKKGEGL+E SDC++CLSEFQENESLRLLPKCSHAFHL CIDTWLKSNS
Sbjct: 121 EALIKSISVFKFKKGEGLVEESDCAICLSEFQENESLRLLPKCSHAFHLACIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCP CRSNI  TNPPP ETPPP VT LGYRHSSDAAVVVVQDLS NVSQE VVVEIV+DS
Sbjct: 181 SCPFCRSNITPTNPPPPETPPPSVTVLGYRHSSDAAVVVVQDLSENVSQEVVVVEIVTDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTER-REESVHSIRSVSEDHHSSPCPGENLSVADVLNI 300
           TN++Q +PNP+PNP S D+GDGRTE+ REES+ SIRSVSEDH SSPCP   LSVAD+L+I
Sbjct: 241 TNRDQGSPNPNPNPPSVDRGDGRTEKNREESIRSIRSVSEDHQSSPCPVRILSVADILSI 300

Query: 301 NHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGIFSFTRYTKG 360
           + EEDEE+EA++S MAA  GPSN + KETEKSRN+N VL    RMSI SG+FSF RY KG
Sbjct: 301 DEEEDEEVEAMNSPMAADTGPSNNDGKETEKSRNRNSVLNSAMRMSIPSGVFSFQRYAKG 360

BLAST of CmoCh08G009820 vs. ExPASy TrEMBL
Match: A0A6J1DSH1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022765 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.5e-150
Identity = 290/376 (77.13%), Postives = 317/376 (84.31%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+S+GI DPSPPFNPFN KDCSQ ICTAFCPQWC ++FPPPPPFFGLDDSDDS TFFSPL
Sbjct: 1   MDSVGIPDPSPPFNPFNLKDCSQGICTAFCPQWCRYVFPPPPPFFGLDDSDDSGTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSV---GDGGGGGPEESFETNRAINETRR-SSAG 120
           IVAVIGILASAFVLVTYYAIVSKYCRR V   G G GGGP + FET+RA+N+ RR S AG
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRRVDSGGGGAGGGPGDYFETDRAMNDARRPSGAG 120

Query: 121 SGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWL 180
            GLDEALIKSISV+KFKK EGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHL CIDTWL
Sbjct: 121 PGLDEALIKSISVFKFKKNEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLVCIDTWL 180

Query: 181 KSNSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEI 240
           KSNSSCPLCR NIASTNP P ETPPP VTALGYRH SDAAVVVVQD S NVSQE+VVVEI
Sbjct: 181 KSNSSCPLCRCNIASTNPNPPETPPPSVTALGYRHRSDAAVVVVQDPSENVSQEAVVVEI 240

Query: 241 ----VSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHS--SPCPGEN 300
               +S+ +NQ+Q   N   NP S +QGDG TERREE+V SIRSVSED  S  SPC G N
Sbjct: 241 IGHSISNPSNQDQGGSN--SNPPSSEQGDGGTERREETVRSIRSVSEDQQSSDSPCLGRN 300

Query: 301 LSVADVLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVL----RMSISSGI 360
           LSVAD+L+I+H+EDEE+EA+DSSMAAGIGPSN + KET KSR +NGVL    RMSI SGI
Sbjct: 301 LSVADILSISHDEDEEVEAVDSSMAAGIGPSNDDGKETGKSRIRNGVLNSAMRMSIPSGI 360

Query: 361 FSFTRYTKGKNSNSPN 363
           FSF ++ KGK+SN PN
Sbjct: 361 FSFAKHAKGKSSNLPN 374

BLAST of CmoCh08G009820 vs. NCBI nr
Match: XP_022964349.1 (RING-H2 finger protein ATL52-like [Cucurbita moschata])

HSP 1 Score: 719.2 bits (1855), Expect = 1.8e-203
Identity = 362/362 (100.00%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of CmoCh08G009820 vs. NCBI nr
Match: KAG6593770.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 717.6 bits (1851), Expect = 5.3e-203
Identity = 361/362 (99.72%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYC+RSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCQRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of CmoCh08G009820 vs. NCBI nr
Match: KAG7026103.1 (RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716.8 bits (1849), Expect = 9.1e-203
Identity = 361/362 (99.72%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           MESIGISDPSPPFNPFNFKDCSQQ CTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MESIGISDPSPPFNPFNFKDCSQQSCTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN
Sbjct: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360

Query: 361 PN 363
           PN
Sbjct: 361 PN 362

BLAST of CmoCh08G009820 vs. NCBI nr
Match: XP_023000254.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 697.6 bits (1799), Expect = 5.7e-197
Identity = 353/362 (97.51%), Postives = 356/362 (98.34%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSV DGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVSDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQGDGRTERREES+HSIRSVSEDHHSSPCPGENLSVADVL+IN
Sbjct: 241 TNQEQEN----PNPRSFDQGDGRTERREESIHSIRSVSEDHHSSPCPGENLSVADVLSIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKN NS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNPNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of CmoCh08G009820 vs. NCBI nr
Match: XP_023513580.1 (RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694.9 bits (1792), Expect = 3.7e-196
Identity = 351/362 (96.96%), Postives = 355/362 (98.07%), Query Frame = 0

Query: 1   MESIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60
           M+SIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL
Sbjct: 1   MDSIGISDPSPPFNPFNFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPL 60

Query: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120
           IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD
Sbjct: 61  IVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRRSSAGSGLD 120

Query: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNS 180
           EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENE+LRLLPKCSHAFHLPCIDTWLKSNS
Sbjct: 121 EALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENENLRLLPKCSHAFHLPCIDTWLKSNS 180

Query: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVVEIVSDS 240
           SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSD AVVVVQDLSANVSQESVVVEIVSDS
Sbjct: 181 SCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDTAVVVVQDLSANVSQESVVVEIVSDS 240

Query: 241 TNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLNIN 300
           TNQEQEN    PNPRSFDQG GRTERREES+HSIRSVSEDHHS+PCPGENLSVADVLNIN
Sbjct: 241 TNQEQEN----PNPRSFDQGHGRTERREESIHSIRSVSEDHHSAPCPGENLSVADVLNIN 300

Query: 301 HEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 360
           HEEDEEIEALDSSMAAGIGPSN NNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS
Sbjct: 301 HEEDEEIEALDSSMAAGIGPSNANNKETEKSRNKNGVLRMSISSGIFSFTRYTKGKNSNS 358

Query: 361 PN 363
           PN
Sbjct: 361 PN 358

BLAST of CmoCh08G009820 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 7.5e-62
Identity = 148/368 (40.22%), Postives = 214/368 (58.15%), Query Frame = 0

Query: 12  PFNPF-NFKDCSQQICTAFCPQWCAFLFPPPPPFFGL-DDSDDSSTFFSPLIVAVIGILA 71
           P++P+ ++ DCSQ IC  +CPQWC  +FPPPPP F L DDS  SS+ FSPL++A+IGIL 
Sbjct: 7   PWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIGILT 66

Query: 72  SAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRAINETRR-SSAGSGLDEALIKSIS 131
           SA +LV+YY ++SKYC R            +       + T+R S+ G GL+E++IKSI+
Sbjct: 67  SALILVSYYTLISKYCHRHHQTSSSETLNLNHNGEGFFSSTQRISTNGDGLNESMIKSIT 126

Query: 132 VYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSN 191
           VYK+K G+G ++GSDCSVCLSEF+ENESLRLLPKC+HAFHLPCIDTWLKS+S+CPLCR+ 
Sbjct: 127 VYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAF 186

Query: 192 IASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQ--------ESVVV--EIVS 251
           +   N P           +  + +S      V +++ N++         +SVVV  ++  
Sbjct: 187 VTGVNNPTASVGQNVSVVVANQSNSAHQTGSVSEINLNLAGYESQTGDFDSVVVIEDLEI 246

Query: 252 DSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVADVLN 311
            S N +  +    P  R       R  + E+S+   RSVS +       G  +S+ADVL 
Sbjct: 247 GSRNSDARSELQLPEER-------RETKDEDSLPIRRSVSLN------SGVVVSIADVLR 306

Query: 312 INHEEDEEIEALDSSMAA-----GIGPSNGNNKETEKSRNKNGVLRMSISSGIFSFTRYT 362
              +E+ E   + +S        G G +    +  ++S   +G    S+S+G F F+RY 
Sbjct: 307 EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLSTGRFIFSRYD 361

BLAST of CmoCh08G009820 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 3.8e-58
Identity = 141/369 (38.21%), Postives = 203/369 (55.01%), Query Frame = 0

Query: 17  NFKDCSQQICTAFCPQWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIGILASAFVLVT 76
           +++DCSQ +C+ +CPQWC  +FPPPP F+  D+ D SS+ FSPL++A+IGILASAF+LV+
Sbjct: 16  SYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVS 75

Query: 77  YYAIVSKYCRRSVGDG------------------GGGGPEESFETNRAINETRRSSAGSG 136
           YY ++SKYC R   +                   G      +  TN   N+T     G G
Sbjct: 76  YYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNP--NQTIGGGGGDG 135

Query: 137 LDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKS 196
           LDE+LIKSI+VYK++K +G +E SDCSVCLSEFQENESLRLLPKC+HAFH+PCIDTWLKS
Sbjct: 136 LDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKS 195

Query: 197 NSSCPLCRSNIASTNPPPQETPPPPVTALGYRHSSDAAVVVVQDLSANVSQESVVV---E 256
           +S+CPLCR+ I +++          V  +   +      +V ++ S +   +SVVV   +
Sbjct: 196 HSNCPLCRAFIVTSS---------AVEIVDLTNQQ----IVTENNSISTGDDSVVVVNLD 255

Query: 257 IVSDSTNQEQENPNPSPNPRSFDQGDGRTERREESVHSIRSVSEDHHSSPCPGENLSVAD 316
           + +  +  E  N   +P P          ERR  S++S              G  +S+AD
Sbjct: 256 LENSRSRNETVNEGSTPKPPEMQDSRDGEERRSASLNS-------------GGGVVSIAD 315

Query: 317 VLNINHEEDEEIEALDSSMAAGIGPSNGNNKETEKSRNK-NGVLRMSISSGIFSFTR--Y 362
           +L    E+DEE   + +S     G        +  + N+ NG+    + S + +  R  Y
Sbjct: 316 ILR-EIEDDEESAGVGTSRWVEEGEGEKTPPPSGSAANQTNGISNFLVRSSMAAMKRSGY 355

BLAST of CmoCh08G009820 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 5.0e-42
Identity = 92/204 (45.10%), Postives = 128/204 (62.75%), Query Frame = 0

Query: 7   SDPSP-PFNPF-NFKDCSQQICTAFCPQWCAFL-FPPPPPFFG--LDDSDDSSTFFSPLI 66
           SDP+P  +N + N +DC+Q +C+ FCPQWC ++ F PPP  +   L+D   S+   SPL+
Sbjct: 3   SDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSPLV 62

Query: 67  VAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGGPEESFETNRA-----INETRR---- 126
           +A+ GI A+AF+L  YY +VSKYC     +      E + E+ R+     +N   R    
Sbjct: 63  IAIFGIFATAFLLAAYYTLVSKYCANDTTN------EAASESGRSDIILDVNSPERGDQD 122

Query: 127 -----SSAGSGLDEALIKSISVYKFKKGEG--LIEGSDCSVCLSEFQENESLRLLPKCSH 186
                 S+ +GLD+ LIK I  +K KK +    I G+DCS+CL EF E+ESLRLLPKC+H
Sbjct: 123 DPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNH 182

Query: 187 AFHLPCIDTWLKSNSSCPLCRSNI 190
            FH+ CID WLKS+S+CPLCR+ I
Sbjct: 183 TFHVVCIDRWLKSHSNCPLCRAKI 200

BLAST of CmoCh08G009820 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.1e-28
Identity = 80/226 (35.40%), Postives = 124/226 (54.87%), Query Frame = 0

Query: 3   SIGISDPSPPFNPF---NFKDCSQQICTAFCPQWCAFLFPPPPP-----FFGLDDSDDSS 62
           S G+ DP  P+N +   ++   S Q+     P W +   P P P        L   D SS
Sbjct: 24  SNGVCDPICPYNCYPEPDYYTISPQL-----PPWSSSPQPSPCPSPSISAVYLPSQDSSS 83

Query: 63  TFFSPLIVAVIGILASAFVLVTYYAIVSKYCRRSVGDGGGGG-----------PEESFET 122
           +  +  I+ + G + +  +L+T + +V+K+   SV     G             EE F+ 
Sbjct: 84  SLDAISIITITGAVLA--ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQD 143

Query: 123 NRAINETRRSSAGSGLDEALIKSISVYKFKKGEGLIEGSDCSVCLSEFQENESLRLLPKC 182
              ++        +GL +++I SI++  +K+G+GLIE +DC VCL+EF+E+ESLRLLPKC
Sbjct: 144 REQVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKC 203

Query: 183 SHAFHLPCIDTWLKSNSSCPLCRSNIASTN-PPPQETPPPPVTALG 209
           +HAFH+ CIDTWL S+++CPLCR+ IA  +   P+ + P  VT  G
Sbjct: 204 NHAFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGG 242

BLAST of CmoCh08G009820 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-27
Identity = 75/176 (42.61%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 32  QWCAFLFPPPPPFFGLDDSDDSSTFFSPLIVAVIG-ILASAFVLVTYYAIVSKYCR---R 91
           ++C   +PPPPP           +   P  + V+G I+   F++   Y  +++  R    
Sbjct: 19  RFCFRQYPPPPP---PPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAA 78

Query: 92  SVGDGGGGGPEESFET-NRAINETRR--SSAGSGLDEALIKSISVYKFKKGEGLIEGSDC 151
           SV  G     E+  ET  R  ++          GL  + I SI+V  FKKGEG+I+G++C
Sbjct: 79  SVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTEC 138

Query: 152 SVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSNSSCPLCRSNI-ASTNPPPQET 200
           SVCL+EF+E+ESLRLLPKCSHAFHL CIDTWL S+ +CPLCR+ +   T PP QET
Sbjct: 139 SVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQET 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF641.1e-6040.22RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ85.4e-5738.21RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY84.4e-5138.50E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0417.1e-4145.10Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q8LFY81.0e-2635.40RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HMW78.9e-204100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464384... [more]
A0A6J1KJE32.8e-19797.51RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494536 P... [more]
A0A6J1H2X81.8e-15679.23RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459995... [more]
A0A6J1L4822.2e-15478.02RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111498996 P... [more]
A0A6J1DSH11.5e-15077.13RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102276... [more]
Match NameE-valueIdentityDescription
XP_022964349.11.8e-203100.00RING-H2 finger protein ATL52-like [Cucurbita moschata][more]
KAG6593770.15.3e-20399.72RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7026103.19.1e-20399.72RING-H2 finger protein ATL51, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023000254.15.7e-19797.51RING-H2 finger protein ATL52-like [Cucurbita maxima][more]
XP_023513580.13.7e-19696.96RING-H2 finger protein ATL52-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G17600.17.5e-6240.22RING/U-box superfamily protein [more]
AT3G03550.13.8e-5838.21RING/U-box superfamily protein [more]
AT4G17905.15.0e-4245.10RING/U-box superfamily protein [more]
AT1G72220.17.1e-2835.40RING/U-box superfamily protein [more]
AT5G10380.13.5e-2742.61RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 144..185
e-value: 6.2E-8
score: 42.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 143..186
e-value: 1.3E-13
score: 51.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 144..186
score: 13.079398
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 119..193
e-value: 2.9E-22
score: 79.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..291
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 314..337
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..279
NoneNo IPR availablePANTHERPTHR45676:SF108E3 UBIQUITIN-PROTEIN LIGASE OS04G0590900-LIKEcoord: 6..245
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 143..186
e-value: 6.87667E-18
score: 74.2605
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 138..192
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 6..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh08G009820.1CmoCh08G009820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane