CmoCh08G009360 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G009360
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	PMEI domain-containing protein
Location	Cmo_Chr08: 6091375 .. 6091935 (+)
RNA-Seq Expression	CmoCh08G009360
Synteny	CmoCh08G009360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCTCTCTCTGTCTCAATTCTTCCCTTCTTGTTCTTCCTCTTCTCCAATTTTGCCATCACTCAATCTTCCATTTCCAACACATCCTCCCTCATCTACAACACTTGCAAGACCAGCTCCGATCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCCAACACGGGGCGGAGCATGGCCGCGGCGCCCTCGATCTCCGCCGCCTCGGCCTCATCTCCCTTCGCTTGGTTCGCCGTAACGTGAGCAGCACACGCCACTATATCAAGAAGTTGCGAAGAAATAAGCACCTCAACACGTTGGTTAAGTCCTGTTTAACTGATTGTTTGGAGCTTTATTCTGATGCCATCCCAACACTTAAGGATGCTCGAAGGGATTATAAGGGTAGACGTTACTTCGAGACGAACTTGAAGATCAGCTCGGTTATGGATGATTGTTCGACTTGTGAAGACGGATTCGAGGAAGAAGGTGTGATTTCACCGTTGACTAATAGGAGTTATAATGCTTTTGAATTATCAGCTATTGCACTCTCCATTATTAATATGCTTTCTTGA

mRNA sequence

ATGTCTTCTCTCTCTGTCTCAATTCTTCCCTTCTTGTTCTTCCTCTTCTCCAATTTTGCCATCACTCAATCTTCCATTTCCAACACATCCTCCCTCATCTACAACACTTGCAAGACCAGCTCCGATCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCCAACACGGGGCGGAGCATGGCCGCGGCGCCCTCGATCTCCGCCGCCTCGGCCTCATCTCCCTTCGCTTGGTTCGCCGTAACGTGAGCAGCACACGCCACTATATCAAGAAGTTGCGAAGAAATAAGCACCTCAACACGTTGGTTAAGTCCTGTTTAACTGATTGTTTGGAGCTTTATTCTGATGCCATCCCAACACTTAAGGATGCTCGAAGGGATTATAAGGGTAGACGTTACTTCGAGACGAACTTGAAGATCAGCTCGGTTATGGATGATTGTTCGACTTGTGAAGACGGATTCGAGGAAGAAGGTGTGATTTCACCGTTGACTAATAGGAGTTATAATGCTTTTGAATTATCAGCTATTGCACTCTCCATTATTAATATGCTTTCTTGA

Coding sequence (CDS)

ATGTCTTCTCTCTCTGTCTCAATTCTTCCCTTCTTGTTCTTCCTCTTCTCCAATTTTGCCATCACTCAATCTTCCATTTCCAACACATCCTCCCTCATCTACAACACTTGCAAGACCAGCTCCGATCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCCAACACGGGGCGGAGCATGGCCGCGGCGCCCTCGATCTCCGCCGCCTCGGCCTCATCTCCCTTCGCTTGGTTCGCCGTAACGTGAGCAGCACACGCCACTATATCAAGAAGTTGCGAAGAAATAAGCACCTCAACACGTTGGTTAAGTCCTGTTTAACTGATTGTTTGGAGCTTTATTCTGATGCCATCCCAACACTTAAGGATGCTCGAAGGGATTATAAGGGTAGACGTTACTTCGAGACGAACTTGAAGATCAGCTCGGTTATGGATGATTGTTCGACTTGTGAAGACGGATTCGAGGAAGAAGGTGTGATTTCACCGTTGACTAATAGGAGTTATAATGCTTTTGAATTATCAGCTATTGCACTCTCCATTATTAATATGCTTTCTTGA

Protein sequence

MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALSIINMLS

Homology

[Close]

BLAST of CmoCh08G009360 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.0e-22
Identity = 60/173 (34.68%), Postives = 111/173 (64.16%), Query Frame = 0


Query: 14  FLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALDLRRLG 73
           F F+   + Q+ IS  + ++  TCK  + + PN++++FC+ SL  GA+      DL+ LG
Sbjct: 10  FFFNLLLLLQAVIS--ADIVQGTCKKVAQRSPNVNYDFCVKSL--GADPKSHTADLQGLG 69

Query: 74  LISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRDYKGRR 133
           +IS  L  ++ S  + +I ++ ++K ++  +K  L DC+ LY+DA  ++++A  D+K + 
Sbjct: 70  VISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKD 129

Query: 134 YFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRSYNAFELSAIALSIINML 186
           Y   N+K+S+ +DD  TCEDGF E++G++SP+T  + +  +L+AI+L+I  +L
Sbjct: 130 YASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of CmoCh08G009360 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.5e-21
Identity = 55/158 (34.81%), Postives = 103/158 (65.19%), Query Frame = 0


Query: 29  TSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALDLRRLGLISLRLVRRNVSSTR 88
           ++ ++  TCK  + + PN++++FC+ SL  GA+    + DL+ LG+IS  L  +  S  +
Sbjct: 14  SADIVQGTCKKVAQRSPNVNYDFCVKSL--GADPKSHSADLQGLGVISANLAIQQGSKIQ 73

Query: 89  HYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRDYKGRRYFETNLKISSVMDDC 148
            +I ++ ++K ++  +K  L DC+ LY+DA  ++++A  D+K + Y   N+K+S+ +DD 
Sbjct: 74  TFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDS 133

Query: 149 STCEDGF-EEEGVISPLTNRSYNAFELSAIALSIINML 186
            TCEDGF E++G+ SP+T  + +  +L+AI+L+I  +L
Sbjct: 134 VTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of CmoCh08G009360 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 47/167 (28.14%), Postives = 85/167 (50.90%), Query Frame = 0


Query: 19  FAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALDLRRLGLISLR 78
           F++  +  +   +LI ++CK +  +DP +S++FC+ SL    +  + A  L  L L S +
Sbjct: 10  FSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQ-SKAATTLESLVLASTK 69

Query: 79  LVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRDYKGRRYFETN 138
                +++ +  + +  +++    +V+  L  CL  Y+DA   L  A  + K R Y   N
Sbjct: 70  TAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRDYQGAN 129

Query: 139 LKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALSIINML 186
           + +S+ +D    CED F+E    SP+TN +   F+   I L+  NML
Sbjct: 130 INLSAALDVPGNCEDDFKEAKKTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL
Match: A0A6J1HKN9 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464419 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 9.2e-96
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60
           MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA
Sbjct: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60

Query: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120
           EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP
Sbjct: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120

Query: 121 TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS 180
           TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS
Sbjct: 121 TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS 180

Query: 181 IINMLS 187
           IINMLS
Sbjct: 181 IINMLS 186

[Close]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL
Match: A0A6J1KIJ0 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 3.0e-86
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHG- 60
           MSS S+SILPFLFFLFSNF ITQSSISNTSSLIY TCKTSSDQDPNISFNFCLTSLQHG 
Sbjct: 1   MSSPSLSILPFLFFLFSNFPITQSSISNTSSLIYKTCKTSSDQDPNISFNFCLTSLQHGV 60

Query: 61  ---AEHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYS 120
              A+HGR ALDLRRLGLISLRLVRRNVSSTRH+IKKL+RNKHLNTLVKSCL DCLELYS
Sbjct: 61  AATAKHGRTALDLRRLGLISLRLVRRNVSSTRHHIKKLQRNKHLNTLVKSCLADCLELYS 120

Query: 121 DAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSA 180
           DAIP LKDARRDYK RRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNR+YN FELSA
Sbjct: 121 DAIPILKDARRDYKDRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRNYNVFELSA 180

Query: 181 IALSIINMLS 187
           IALSIINMLS
Sbjct: 181 IALSIINMLS 190

[Close]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL
Match: A0A0A0KBR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.4e-59
Identity = 137/192 (71.35%), Postives = 158/192 (82.29%), Query Frame = 0


Query: 1   MSSLSVSILP--FLFFLFSNFAITQSS-ISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQ 60
           MSSLS SILP  FLF  FSNF ITQSS  +NTSSLIY TCK SS+QDPNISFNFC+TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 61  HGA-EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKK-LRRNK-HLNTLVKSCLTDCLEL 120
             A +H  G   LRRLGLI++ L+R N+S+TRH+IKK L +NK   +  VK CLTDCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 121 YSDAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRSYNAFE 180
           YSDAIPT+K AR+DYK  RY + NLKISSVMDDCSTCE+GF E++GVISPLTNR++NAFE
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFE 180

Query: 181 LSAIALSIINML 186
           LSAIALSIINML
Sbjct: 181 LSAIALSIINML 192

[Close]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL
Match: A0A6J1H3N0 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111460113 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 130/189 (68.78%), Postives = 152/189 (80.42%), Query Frame = 0


Query: 1   MSSLSVSILP--FLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQH 60
           MSSLS+SI+P  FLF  FS+  ITQSS +N SSL Y TCKTSSDQ+PNIS NFCLTSL  
Sbjct: 1   MSSLSLSIIPLLFLFMFFSDLPITQSS-NNKSSLTYKTCKTSSDQNPNISLNFCLTSL-- 60

Query: 61  GAEHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHL-NTLVKSCLTDCLELYSD 120
               G GA  LRRLGL+S+RL+RRNVS+TRH IKKLR+NK L + L+K CLTDCLELYSD
Sbjct: 61  ----GHGATSLRRLGLLSIRLIRRNVSTTRHLIKKLRKNKRLDDPLIKLCLTDCLELYSD 120

Query: 121 AIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAI 180
           A   +K AR+DYK  RYFE NL I+SVM+ CSTCEDGF+++ VISPLTN ++N FELSAI
Sbjct: 121 ATLMVKQARKDYKAERYFEANLMITSVMNGCSTCEDGFKKKMVISPLTNMNHNVFELSAI 180

Query: 181 ALSIINMLS 187
           ALSIINML+
Sbjct: 181 ALSIINMLA 182

[Close]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL
Match: A0A1S3CBM6 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498982 PE=4 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 6.4e-57
Identity = 134/194 (69.07%), Postives = 156/194 (80.41%), Query Frame = 0


Query: 1   MSSLSVSIL-PFLF---FLFSNFAITQSS--ISNTSSLIYNTCKTSSDQDPNISFNFCLT 60
           MSSLS SIL P LF    +FSNF ITQSS   +NTSSLIY TCK SS+QDPNISF+FC+T
Sbjct: 1   MSSLSHSILIPCLFLFMLIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCIT 60

Query: 61  SLQHGA-EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHL-NTLVKSCLTDCL 120
           SL+  A +H  G   LRRLGL+++ L+R N+SSTRH+IKKL+R K   +  VK CLTDCL
Sbjct: 61  SLKPAATKHRHGDTSLRRLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCL 120

Query: 121 ELYSDAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEE-EGVISPLTNRSYNA 180
           ELYSDAIP +K AR+DYK  RY + N KISSVMDDCSTCE+GFEE EGV+SPLTNR++NA
Sbjct: 121 ELYSDAIPMVKQARKDYKAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNA 180

Query: 181 FELSAIALSIINML 186
           FELSAIALSIINML
Sbjct: 181 FELSAIALSIINML 194

[Close]

BLAST of CmoCh08G009360 vs. NCBI nr
Match: XP_022964388.1 (putative invertase inhibitor [Cucurbita moschata] >KAG7026060.1 hypothetical protein SDJN02_12558, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 359.4 bits (921), Expect = 1.9e-95
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60
           MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA
Sbjct: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60

Query: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120
           EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP
Sbjct: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120

Query: 121 TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS 180
           TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS
Sbjct: 121 TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS 180

Query: 181 IINMLS 187
           IINMLS
Sbjct: 181 IINMLS 186

[Close]

BLAST of CmoCh08G009360 vs. NCBI nr
Match: KAG6593721.1 (hypothetical protein SDJN03_13197, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 183/186 (98.39%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60
           MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA
Sbjct: 41  MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 100

Query: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120
           EHGRGALDLRRLGL+SLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP
Sbjct: 101 EHGRGALDLRRLGLVSLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 160

Query: 121 TLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALS 180
           TLKDARRDYKG+RYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNR+YNAFELSAIALS
Sbjct: 161 TLKDARRDYKGKRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRNYNAFELSAIALS 220

Query: 181 IINMLS 187
           IINMLS
Sbjct: 221 IINMLS 226

[Close]

BLAST of CmoCh08G009360 vs. NCBI nr
Match: XP_023514422.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 2.9e-88
Identity = 176/190 (92.63%), Postives = 182/190 (95.79%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHG- 60
           MSSLS+SILP LFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSL+HG 
Sbjct: 1   MSSLSLSILPLLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLRHGV 60

Query: 61  ---AEHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYS 120
              A+HGR ALDLRRLGLISLRLVRRNVSSTRH+IKKLRRNKHLNTLVKSCL DCLELYS
Sbjct: 61  VAAAKHGRSALDLRRLGLISLRLVRRNVSSTRHHIKKLRRNKHLNTLVKSCLADCLELYS 120

Query: 121 DAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSA 180
           DAIP LKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNR++NAFELSA
Sbjct: 121 DAIPILKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRNHNAFELSA 180

Query: 181 IALSIINMLS 187
           IALSIINMLS
Sbjct: 181 IALSIINMLS 190

[Close]

BLAST of CmoCh08G009360 vs. NCBI nr
Match: XP_022999944.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 327.8 bits (839), Expect = 6.1e-86
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHG- 60
           MSS S+SILPFLFFLFSNF ITQSSISNTSSLIY TCKTSSDQDPNISFNFCLTSLQHG 
Sbjct: 1   MSSPSLSILPFLFFLFSNFPITQSSISNTSSLIYKTCKTSSDQDPNISFNFCLTSLQHGV 60

Query: 61  ---AEHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYS 120
              A+HGR ALDLRRLGLISLRLVRRNVSSTRH+IKKL+RNKHLNTLVKSCL DCLELYS
Sbjct: 61  AATAKHGRTALDLRRLGLISLRLVRRNVSSTRHHIKKLQRNKHLNTLVKSCLADCLELYS 120

Query: 121 DAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSA 180
           DAIP LKDARRDYK RRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNR+YN FELSA
Sbjct: 121 DAIPILKDARRDYKDRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRNYNVFELSA 180

Query: 181 IALSIINMLS 187
           IALSIINMLS
Sbjct: 181 IALSIINMLS 190

[Close]

BLAST of CmoCh08G009360 vs. NCBI nr
Match: XP_038906889.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 245.7 bits (626), Expect = 3.1e-61
Identity = 139/190 (73.16%), Postives = 156/190 (82.11%), Query Frame = 0


Query: 1   MSSLSVSILP--FLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQH 60
           MSSLS SIL   FLF  FSNF I +SS +NT SL+Y TCKT SDQDPNISFNFCLTSL  
Sbjct: 1   MSSLSHSILRFLFLFMFFSNFLIIKSS-NNTLSLLYKTCKTISDQDPNISFNFCLTSLVP 60

Query: 61  GA-EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHL-NTLVKSCLTDCLELYS 120
            A +HG GA  LRRLGLI++ L+RRNVSSTRH+IK LRRNK   + LVKSCL DCLELYS
Sbjct: 61  AATKHGHGATTLRRLGLITIYLIRRNVSSTRHHIKNLRRNKEPGDPLVKSCLADCLELYS 120

Query: 121 DAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSA 180
           DA  T+K AR+DYK RRY + +  ISSVMDDCSTCEDGF+EEGVISPLTNR++NAFELSA
Sbjct: 121 DAALTMKQARKDYKARRYDDASSMISSVMDDCSTCEDGFKEEGVISPLTNRNHNAFELSA 180

Query: 181 IALSIINMLS 187
           IALSIINML+
Sbjct: 181 IALSIINMLA 189

[Close]

BLAST of CmoCh08G009360 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 8.1e-20
Identity = 57/177 (32.20%), Postives = 96/177 (54.24%), Query Frame = 0


Query: 9   LPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALD 68
           + FL +L + F +  + ++N  +LI N+CK ++   P   +N C+TSL+   +  + A D
Sbjct: 1   MKFLLYLVT-FFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQ-AKTAKD 60

Query: 69  LRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRD 128
           L  L + S +      ++ +  + K+ + K +N +    L DCL+LY+DAI +L +A   
Sbjct: 61  LAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAG 120

Query: 129 YKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPLTNRSYNAFELSAIALSIINML 186
            K R Y      +S+ MD  STCE GF+E    SP+T  + N +++  I L+  NML
Sbjct: 121 VKSRNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKENDNLYQMILIPLAFTNML 175

[Close]

BLAST of CmoCh08G009360 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.4e-16
Identity = 64/198 (32.32%), Postives = 98/198 (49.49%), Query Frame = 0


Query: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60
           M    V  + FLFFL        S + +TS +I  TCK+ + +     +NFC++SL +  
Sbjct: 1   MKLSQVFYIIFLFFLV-------SQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSP 60

Query: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIP 120
                  +L  L L+ +     N ++T   I++L  +    +   +CL DCLELY DA  
Sbjct: 61  IALPSPTNLSSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATD 120

Query: 121 TLKDARRDYKGRRYFET-NLKISSVMDDCSTCEDGFEEE--------GV---ISPLTNRS 180
            L++A R +  R+   T N+ +S+ M+   TCE+GF E         GV    SP+ + +
Sbjct: 121 RLEEAVRVFITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDEN 180

Query: 181 YNAFELSAIALSIINMLS 187
           +  FE   IAL I NMLS
Sbjct: 181 HKLFEFGQIALCIFNMLS 191

[Close]

BLAST of CmoCh08G009360 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 50/149 (33.56%), Postives = 82/149 (55.03%), Query Frame = 0


Query: 9   LPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEHGRGALD 68
           + FLF+ F+ F +  ++ +   SLI + CK ++D++P I +NFC+ SL+   +  + A  
Sbjct: 1   MTFLFY-FAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQ-SKTARS 60

Query: 69  LRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTLKDARRD 128
           L RL ++S +      +S +  + K+ +        +  L DCLELYSDA  +LK+A   
Sbjct: 61  LDRLVMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTI 120

Query: 129 YKGRRYFETNLKISSVMDDCSTCEDGFEE 158
            K R Y   N+ IS+ M    +CE GF+E
Sbjct: 121 IKSRDYKTANVVISAAMGAPPSCEIGFKE 147

[Close]

BLAST of CmoCh08G009360 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.3e-11
Identity = 53/185 (28.65%), Postives = 91/185 (49.19%), Query Frame = 0


Query: 4   LSVSILPFLFFLFSN-FAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGAEH 63
           +  S+   +FFL  N FA  Q       SLI ++CK ++ +DP + ++FC+ SL+   + 
Sbjct: 1   MKFSLYLVMFFLLLNCFATAQ-------SLIRDSCKKATTKDPKLKYDFCVKSLEENPQ- 60

Query: 64  GRGALDLRRLGLISLRLVRRNVSSTRHYIKKLRRNKHLNTLVKSCLTDCLELYSDAIPTL 123
            + A  L  L  +S +      +S +  + K+ +       V+  L DCLELY++AI +L
Sbjct: 61  SKTAKSLEGLVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSL 120

Query: 124 KDARRDYKGRRYFETNLKISSVMDDCSTCEDGF--EEEGVISPLTNRSYNAFELSAIALS 183
             +    K R Y    + +S+ MD   +CE  F   ++ V SP T  +   F +  I ++
Sbjct: 121 NQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIA 175

Query: 184 IINML 186
             +ML
Sbjct: 181 FTSML 175

[Close]

BLAST of CmoCh08G009360 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 9.3e-08
Identity = 26/56 (46.43%), Postives = 32/56 (57.14%), Query Frame = 0


Query: 109 TDCLELYSDAIPTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGFEEEGVISPL 165
           +DC ELY DA+  L+DA    K       N  +S+ M D STC DGFEE G  +PL
Sbjct: 187 SDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPL 242

The following BLAST results are available for this feature:

BLAST of CmoCh08G009360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	5.0e-22	34.68	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	2.5e-21	34.81	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	2.1e-12	28.14	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

BLAST of CmoCh08G009360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HKN9	9.2e-96	100.00	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464419 PE=4 ...	[more]
A0A6J1KIJ0	3.0e-86	91.05	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV...	[more]
A0A0A0KBR8	2.4e-59	71.35	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 S...	[more]
A0A6J1H3N0	1.3e-57	68.78	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111460113 PE=4 ...	[more]
A0A1S3CBM6	6.4e-57	69.07	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498982 PE=4 SV=1	[more]

BLAST of CmoCh08G009360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022964388.1	1.9e-95	100.00	putative invertase inhibitor [Cucurbita moschata] >KAG7026060.1 hypothetical pro...	[more]
KAG6593721.1	1.2e-94	98.39	hypothetical protein SDJN03_13197, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023514422.1	2.9e-88	92.63	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_022999944.1	6.1e-86	91.05	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_038906889.1	3.1e-61	73.16	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of CmoCh08G009360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	8.1e-20	32.20	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	2.4e-16	32.32	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	3.5e-15	33.56	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	5.3e-11	28.65	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	9.3e-08	46.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..181 e-value: 9.8E-23 score: 91.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..185 e-value: 1.9E-36 score: 123.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..181 e-value: 3.6E-22 score: 79.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..186 e-value: 4.1E-32 score: 113.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..185
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 28..185
None	No IPR available	PANTHER	PTHR31080:SF226	PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATED	coord: 28..185
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 37..184 e-value: 1.59747E-37 score: 124.785

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G009360.1	CmoCh08G009360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity