CmoCh07G007950 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh07G007950
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	crossover junction endonuclease EME1B-like isoform X1
Location	Cmo_Chr07: 3639776 .. 3643120 (-)
RNA-Seq Expression	CmoCh07G007950
Synteny	CmoCh07G007950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGTTCTGTTTCATTTAGAAGAAAATATATTTATTACATGCTCTTTGCAATTTCAGTATCTTTGTCCTGGTTTTTCTTCATTAATTGTTGCTTGATAACTTGAAGTTGCGTGCTAGAGATTCTGTAATTTTTAACGTTTGAACTTTGTAGGGAACAGGAGAAGTACAAGAACTCAGAAAGCAATAACGGTTGAAAACGTCCACCTGTTGAGGAGGTTTGAATAACAATCATGAATGCTTAGGAGAATTAGGAAATGATTTTACCCTGCTTTCCCTAGCCAAGTTTTATACTTCAATGTTGCAGTATAAAATCTCTCATGTTCCTCACATGTTTCATCTAGAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGGTTCTGAATACTGTTCTCAAATTTCTATCAAGCATAACTTCTTGAAGTGTGAAAAGTTATTTTTCTGGATAACTTGGCGTCAATTGCAAATGTATAACTTAAATTTGTGTCGTGAAGTTTGGTCAAGTAGAAAGGATATTTCATATACTGTTCGTCGATCCCATAGACTGAACATTGTATGAAAAGAAGCCAAGAGATTCCTGTTTCGTACATCTTTAGATGTTGCATTAGTTCCATAGTTTTTTTGACATGGTACGTTAGTAAATATGGACTTAGGCGATCACACCCATATACACACTCTTTCACATATTTAAATCCTTAGTCAGAATTATCTATTTTCCTTTGCTTGTCTTGTAAAATTGAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCACAGGAATGTGATAGAGAAGTGCGTGAGGTGAGCTTTAATCGTCAATGGCCAATTCATTTCAAAATTTATTGGCATTCGGTTTCTTAATTACTCTGGCATTTACTTGATTTATATTAATGAATTTATGGCTGTAATTGAGTTACTCTCGAAGAACTCTTGTCTGTCACCAAACTGGCGGGTTAAAAGCTAAACAGAGCACTTGTCATGTATTTGATCTCAGCATCAGTCTCGTTTATGTCACAACAATATTCTAAACCATAAGAAACTATGAAACTAATATCTATCCTTCTTTGCTTTTTTGTACTTAATTACCCTTGTTGATAGATAACTGTAAGACTTTTAGTAATAAAATCATGTTATACCACCACTAAAATTATTATATACCATGTTATACCACCACTAAAATTATTATATACCATGAACTTAGTTCAATAGTTAAGGCGTTTCTACTTTTCCTAAGAGGTTATATGTTTGACTTCTTTTATCCCAAATGTGATGATGTATTGAACTTAAAAAAGTTATATGGTACTGTCTCTGAACTAAAACTTTATTGATTTTGTCATGTTTCTTTTTGTAATTAGGTTAGAAGCCTTGGTAGCAATTCTTATAATTAGCTTGGTTGGGATTGCAAATCAATATAAATGGAGTTGCTTTTTGTAATTAGGTTAGAAGTCTTGGTAGCAATTCCAAAGGTACAACCACGGTTTTCCGTACCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAGAGTGTACATGAATCCATGTAAATCTGTGAGTCTGAATCGAACAATTCACCTTATCCTCAATTGCATAAGCTAGCAGTCATGGCAGATCACATTTCTTCTTGGTCCACTCATGTAATAACAGGTGCATGAAAAGGAATTTCTGCTGAAAGACTGGACGACAGAAGGTTTACTCGGCAATGATAGAAGGTTAGGAGAGATTTGTTCGAAGAGAGTGTACAGGATACTTATGGCTCAAAGTGGAAGCATGAGAACTGATGACGTTGAGGAGGGTGATGACTTGTTCAGATCCTAATTGTCTTAATTAATTATCATATCTAATTAGCACACCATTATTTTAATGCAATAGCTTATTGCAGAGTTTTCACCCTCGCAGACAGGTTGAAGGACTAGAGTGCCCTCATCTCTTTATCACAAATGCCACTTGGAGGCAGCGACTCGTGGAGCAAAGGGTAAATAAGTAAGTTTTCGTTGTTCAATATCCAAATCTGAATTCTCCCATCAACAAGGTAGAACTGTCCAAAATGAACTTCTTGGCTGGAGTACAATTAATTTTTCTTGCTTTTTAGTAGAGTAATTTCAACTAGGTGGGAAACCAGCATTTCACCTTGATGTTCAGTGACATAGTGTTGCACGATCCCATGTTGGTTGGAGAGGAGAACAAAACACCCTTTATAAGGGTGTGGAAACCTTTCCCTAGCTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGTCCAAAAAGGACAAATATCTGCTAGCGGTGGGCTTGGGTGGTTACATATATACCTCTTTCCTTATAATTTGTCCAAAGTGCATTACAATGTTAGGTTATAGAGACGAACGTATTGACTAATCGTTCCTTGTTCTTTTAACCGTAGTTAGAAGTGAATTGATCATCTATAGAAAGATTTCTATGTAAAGTGAACATAGTTTGATATGGTATCAGAATTCAAAGGGCTAATTGCTACTTTATATTGTGTGAGTTAGTTCAAGTTTGGCATTTTTTGGTGGGAGGTTTGGTTTGGTAGTAGTAACGGTATGGTTAGGTAGAAGTTTTGATATCCTAAAAGTAGGGAGAATATTGAATTATACCAAAAAAGAAGAGACTCAAGGTTGATATCAATAATGACAACAATTTGGTGGTACTATTTTTTCTCATCCCCACCCTGCTTCCTCATATTCCAATTTTATGTGGTTCGAAATCCATCAGAAGCATTGCATTCAAACAAGTGGATTGCTCACTTTGTAGGGTTTGTGTAATTTCCAACAGAGAGCCAAAATTTTGATGATTGTGACCCAAATCCAATTAAATTATGGTTGGGCATGGCCATTCCTCTAATCCAATTCACACTCATCCTAATTCCACCACACAAAACACCCACTAATGGGTGCTGCCACCAGCTCGCACCACTTTTGTTTTTTGAATGCCTCTTTCACCCAAGGAAATCTTACCTGAATGTATAATTGATCTCTTTTAAAGTTCATGAATTATTTTTGGACAAAGGTAGAAATGGGATTGGAAGTTTTTAATACTAAAGAAGATCTTGATTAATTGATTTACCGGATCAATCATTAGTTAATTATGTTTTTATATTGTTTTGGATTAGGTAATTGTTGCATCTTTCATAGAAGATCTCTGGACATTAGAATATAGGAAATTAAAATTCACATTCGCAATACTCATTTTGGTGCTTCGCACTTTCGCAAAGTGA

mRNA sequence

ATGAAAGTTCTGTTTCATTTAGAAGAAAATATATTTATTACATGCTCTTTGCAATTTCAGTATCTTTGTCCTGGGAACAGGAGAAGTACAAGAACTCAGAAAGCAATAACGAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGTCAGAATTATCTATTTTCCTTTGCTTGTCTTGTAAAATTGAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCACAGGAATGTGATAGAGAAGTGCGTGAGGTTAGAAGTCTTGGTAGCAATTCCAAAGGTACAACCACGGTTTTCCGTACCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAGAGTGTACATGAATCCATGTAAATCTGTGCATGAAAAGGAATTTCTGCTGAAAGACTGGACGACAGAAGGTTTACTCGGCAATGATAGAAGGTTAGGAGAGATTTGTTCGAAGAGAGTGTACAGGATACTTATGGCTCAAAGTGGAAGCATGAGAACTGATGACAGTTTTCACCCTCGCAGACAGGTTGAAGGACTAGAGTGCCCTCATCTCTTTATCACAAATGCCACTTGGAGGCAGCGACTCGTGGAGCAAAGGGTAATTGTTGCATCTTTCATAGAAGATCTCTGGACATTAGAATATAGGAAATTAAAATTCACATTCGCAATACTCATTTTGGTGCTTCGCACTTTCGCAAAGTGA

Coding sequence (CDS)

ATGAAAGTTCTGTTTCATTTAGAAGAAAATATATTTATTACATGCTCTTTGCAATTTCAGTATCTTTGTCCTGGGAACAGGAGAAGTACAAGAACTCAGAAAGCAATAACGAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGTCAGAATTATCTATTTTCCTTTGCTTGTCTTGTAAAATTGAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCACAGGAATGTGATAGAGAAGTGCGTGAGGTTAGAAGTCTTGGTAGCAATTCCAAAGGTACAACCACGGTTTTCCGTACCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAGAGTGTACATGAATCCATGTAAATCTGTGCATGAAAAGGAATTTCTGCTGAAAGACTGGACGACAGAAGGTTTACTCGGCAATGATAGAAGGTTAGGAGAGATTTGTTCGAAGAGAGTGTACAGGATACTTATGGCTCAAAGTGGAAGCATGAGAACTGATGACAGTTTTCACCCTCGCAGACAGGTTGAAGGACTAGAGTGCCCTCATCTCTTTATCACAAATGCCACTTGGAGGCAGCGACTCGTGGAGCAAAGGGTAATTGTTGCATCTTTCATAGAAGATCTCTGGACATTAGAATATAGGAAATTAAAATTCACATTCGCAATACTCATTTTGGTGCTTCGCACTTTCGCAAAGTGA

Protein sequence

MKVLFHLEENIFITCSLQFQYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDDSFHPRRQVEGLECPHLFITNATWRQRLVEQRVIVASFIEDLWTLEYRKLKFTFAILILVLRTFAK

Homology

[Close]

BLAST of CmoCh07G007950 vs. ExPASy Swiss-Prot
Match: C5H8J1 (Crossover junction endonuclease EME1B OS=Arabidopsis thaliana OX=3702 GN=EME1B PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.1e-46
Identity = 102/186 (54.84%), Postives = 129/186 (69.35%), Query Frame = 0


Query: 20  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQN 79
           +Y  PGN R     +    LAKLTTH+VKV SR CVDE E A+    LT SLAS QF   
Sbjct: 368 EYKNPGNWRRPPIDEV---LAKLTTHYVKVHSRHCVDEAEVAEHIVGLTSSLASCQF--- 427

Query: 80  YLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPI 139
                      RKKLT LSV+A G+++ KD V +++I+K   L+ LVAIPKVQPR+++ +
Sbjct: 428 -----------RKKLTMLSVSANGALVSKDSVDKHLIKKSPWLKALVAIPKVQPRYALAV 487

Query: 140 WKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSG 199
           WKKYP+MKS  +VYM+  KSVHEKEFLLKD   EGL+G D RLGEICSKR+YR+LM+  G
Sbjct: 488 WKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKVEGLVGGDIRLGEICSKRIYRVLMSHDG 536

Query: 200 SMRTDD 203
           +++TDD
Sbjct: 548 AIKTDD 536

[Close]

BLAST of CmoCh07G007950 vs. ExPASy Swiss-Prot
Match: Q84M98 (Crossover junction endonuclease EME1A OS=Arabidopsis thaliana OX=3702 GN=EME1A PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-38
Identity = 88/186 (47.31%), Postives = 120/186 (64.52%), Query Frame = 0


Query: 20  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQN 79
           +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q    
Sbjct: 359 EYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQ---- 418

Query: 80  YLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPI 139
                     +RKKLT LSV A G+++ K+   +++I + + L+VLVAIPKVQPR+++ +
Sbjct: 419 ----------VRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLVAIPKVQPRYAIAV 478

Query: 140 WKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSG 199
            KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G
Sbjct: 479 SKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEACSKRIYRVLMSLDG 530

Query: 200 SMRTDD 203
           +++TDD
Sbjct: 539 TIKTDD 530

[Close]

BLAST of CmoCh07G007950 vs. ExPASy Swiss-Prot
Match: Q0J9J6 (Crossover junction endonuclease EME1 OS=Oryza sativa subsp. japonica OX=39947 GN=EME1 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-30
Identity = 84/180 (46.67%), Postives = 106/180 (58.89%), Query Frame = 0


Query: 26  NRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFA 85
           N  S +       L KL TH+ +VRSR C DE E  +    LT SLA+ +F         
Sbjct: 479 NSNSWKRPPVEEALCKLATHYARVRSRHCTDEAEVTEHIVGLTYSLANCKF--------- 538

Query: 86  CLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPT 145
                RK LT LSV+A GS I K CV ++ I+K   L+ LVAIP V P  ++ I KKYP+
Sbjct: 539 -----RKPLTWLSVHANGSNISKGCVDKDRIKKSAWLKSLVAIPGVSPGQAIAIEKKYPS 598

Query: 146 MKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGN-DRRLGEICSKRVYRILMAQSGSMRTD 202
           M+S   VYM+  KSVHEKE LL+D   EG LG+  RRLG  CSK+VY ILMAQ+G+   +
Sbjct: 599 MRSLLNVYMDDSKSVHEKEHLLEDLRLEGPLGDFKRRLGPACSKKVYTILMAQNGAAEVE 644

[Close]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL
Match: A0A6J1IRV2 (crossover junction endonuclease EME1B isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.0e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 396 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 455

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 456 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 515

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 516 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 549

[Close]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL
Match: A0A6J1IQH9 (crossover junction endonuclease EME1B isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.0e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 400 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 459

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 460 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 519

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 520 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 553

[Close]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL
Match: A0A6J1IIZ2 (crossover junction endonuclease EME1B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.0e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 403 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 462

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 463 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 522

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 523 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 556

[Close]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL
Match: A0A6J1GG33 (crossover junction endonuclease EME1B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453614 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.0e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 400 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 459

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 460 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 519

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 520 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 553

[Close]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL
Match: A0A6J1GF29 (crossover junction endonuclease EME1B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453614 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 2.0e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 403 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 462

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 463 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 522

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 523 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 556

[Close]

BLAST of CmoCh07G007950 vs. NCBI nr
Match: XP_022977602.1 (crossover junction endonuclease EME1B isoform X1 [Cucurbita maxima])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 403 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 462

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 463 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 522

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 523 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 556

[Close]

BLAST of CmoCh07G007950 vs. NCBI nr
Match: XP_022950534.1 (crossover junction endonuclease EME1B-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 400 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 459

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 460 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 519

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 520 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 553

[Close]

BLAST of CmoCh07G007950 vs. NCBI nr
Match: XP_022977604.1 (crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 396 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 455

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 456 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 515

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 516 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 549

[Close]

BLAST of CmoCh07G007950 vs. NCBI nr
Match: XP_022950535.1 (crossover junction endonuclease EME1B-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 389 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 448

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 449 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 508

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 509 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 542

[Close]

BLAST of CmoCh07G007950 vs. NCBI nr
Match: XP_022977603.1 (crossover junction endonuclease EME1B isoform X2 [Cucurbita maxima])

HSP 1 Score: 239.2 bits (609), Expect = 4.1e-59
Identity = 126/168 (75.00%), Postives = 136/168 (80.95%), Query Frame = 0


Query: 38  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHL 97
           +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHL
Sbjct: 400 ELAKLTTHFVKVRSRQCVDEAEVADHIVGLTCSLASCQF--------------RKKLTHL 459

Query: 98  SVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPC 157
           SVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V IWKKYPTMKS   VYM+PC
Sbjct: 460 SVNANGSIIPKDCIDRNMIKKSLWLKALVAIPKVQPRFAVAIWKKYPTMKSLLSVYMDPC 519

Query: 158 KSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD 203
           KSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Sbjct: 520 KSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKRVYRILMAQSGSMRTDD 553

[Close]

BLAST of CmoCh07G007950 vs. TAIR 10
Match: AT2G22140.1 (essential meiotic endonuclease 1B )

HSP 1 Score: 186.4 bits (472), Expect = 2.9e-47
Identity = 102/186 (54.84%), Postives = 129/186 (69.35%), Query Frame = 0


Query: 20  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQN 79
           +Y  PGN R     +    LAKLTTH+VKV SR CVDE E A+    LT SLAS QF   
Sbjct: 368 EYKNPGNWRRPPIDEV---LAKLTTHYVKVHSRHCVDEAEVAEHIVGLTSSLASCQF--- 427

Query: 80  YLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPI 139
                      RKKLT LSV+A G+++ KD V +++I+K   L+ LVAIPKVQPR+++ +
Sbjct: 428 -----------RKKLTMLSVSANGALVSKDSVDKHLIKKSPWLKALVAIPKVQPRYALAV 487

Query: 140 WKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSG 199
           WKKYP+MKS  +VYM+  KSVHEKEFLLKD   EGL+G D RLGEICSKR+YR+LM+  G
Sbjct: 488 WKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKVEGLVGGDIRLGEICSKRIYRVLMSHDG 536

Query: 200 SMRTDD 203
           +++TDD
Sbjct: 548 AIKTDD 536

[Close]

BLAST of CmoCh07G007950 vs. TAIR 10
Match: AT2G21800.1 (essential meiotic endonuclease 1A )

HSP 1 Score: 161.4 bits (407), Expect = 1.0e-39
Identity = 88/186 (47.31%), Postives = 120/186 (64.52%), Query Frame = 0


Query: 20  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQN 79
           +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q    
Sbjct: 359 EYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQ---- 418

Query: 80  YLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPI 139
                     +RKKLT LSV A G+++ K+   +++I + + L+VLVAIPKVQPR+++ +
Sbjct: 419 ----------VRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLVAIPKVQPRYAIAV 478

Query: 140 WKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSG 199
            KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G
Sbjct: 479 SKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEACSKRIYRVLMSLDG 530

Query: 200 SMRTDD 203
           +++TDD
Sbjct: 539 TIKTDD 530

[Close]

BLAST of CmoCh07G007950 vs. TAIR 10
Match: AT2G21800.2 (essential meiotic endonuclease 1A )

HSP 1 Score: 161.4 bits (407), Expect = 1.0e-39
Identity = 88/186 (47.31%), Postives = 120/186 (64.52%), Query Frame = 0


Query: 20  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQN 79
           +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q    
Sbjct: 362 EYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQ---- 421

Query: 80  YLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSVPI 139
                     +RKKLT LSV A G+++ K+   +++I + + L+VLVAIPKVQPR+++ +
Sbjct: 422 ----------VRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLVAIPKVQPRYAIAV 481

Query: 140 WKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSG 199
            KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G
Sbjct: 482 SKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEACSKRIYRVLMSLDG 533

Query: 200 SMRTDD 203
           +++TDD
Sbjct: 542 TIKTDD 533

The following BLAST results are available for this feature:

BLAST of CmoCh07G007950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C5H8J1	4.1e-46	54.84	Crossover junction endonuclease EME1B OS=Arabidopsis thaliana OX=3702 GN=EME1B P...	[more]
Q84M98	1.4e-38	47.31	Crossover junction endonuclease EME1A OS=Arabidopsis thaliana OX=3702 GN=EME1A P...	[more]
Q0J9J6	1.9e-30	46.67	Crossover junction endonuclease EME1 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]

BLAST of CmoCh07G007950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IRV2	2.0e-59	75.00	crossover junction endonuclease EME1B isoform X3 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1IQH9	2.0e-59	75.00	crossover junction endonuclease EME1B isoform X2 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1IIZ2	2.0e-59	75.00	crossover junction endonuclease EME1B isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GG33	2.0e-59	75.00	crossover junction endonuclease EME1B-like isoform X2 OS=Cucurbita moschata OX=3...	[more]
A0A6J1GF29	2.0e-59	75.00	crossover junction endonuclease EME1B-like isoform X1 OS=Cucurbita moschata OX=3...	[more]

BLAST of CmoCh07G007950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977602.1	4.1e-59	75.00	crossover junction endonuclease EME1B isoform X1 [Cucurbita maxima]	[more]
XP_022950534.1	4.1e-59	75.00	crossover junction endonuclease EME1B-like isoform X2 [Cucurbita moschata]	[more]
XP_022977604.1	4.1e-59	75.00	crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima]	[more]
XP_022950535.1	4.1e-59	75.00	crossover junction endonuclease EME1B-like isoform X3 [Cucurbita moschata]	[more]
XP_022977603.1	4.1e-59	75.00	crossover junction endonuclease EME1B isoform X2 [Cucurbita maxima]	[more]

BLAST of CmoCh07G007950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22140.1	2.9e-47	54.84	essential meiotic endonuclease 1B	[more]
AT2G21800.1	1.0e-39	47.31	essential meiotic endonuclease 1A	[more]
AT2G21800.2	1.0e-39	47.31	essential meiotic endonuclease 1A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042530	EME1/EME2, C-terminal domain	GENE3D	1.10.150.670		coord: 115..203 e-value: 8.8E-14 score: 53.7
IPR033310	Mms4/EME1/EME2	PANTHER	PTHR21077	EME1 PROTEIN	coord: 19..202
None	No IPR available	PANTHER	PTHR21077:SF5	METHYL METHANESULFONATE SENSITIVITY 4	coord: 19..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh07G007950.1	CmoCh07G007950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0051301	cell division
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0048476	Holliday junction resolvase complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004519	endonuclease activity