CmoCh05G014040 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh05G014040
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pre-mRNA cleavage factor Im 25 kDa subunit
Location	Cmo_Chr05: 10820281 .. 10822539 (-)
RNA-Seq Expression	CmoCh05G014040
Synteny	CmoCh05G014040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCACTCTCTGCAACCACACGCCATCTTCAAACATCCAGACCTATATTCTCTACTCCCACTCACTTTCCTCCTCTCTCTCCTCTCCATATTCGATTCTCTCTATCTCTCTCTGTTTTTCTGCAATCGGCCATGGGAGACGACGGACATGTAAATGCTATGGAGGACGATGAAGCTTCTGCCGCCGAGCAACGCGGAGACATCGACATTTACCCTCTCAGAAACTACTTCTTTGGATCCAAAGAGGCTCTGCTTTTCAAGAACGAGGCCTTGGCTGATCGCGTCCAGAGGATGAATTCCAAGTATCTATTCTCCCTTTAATCTCTTCACTACACTGCCTGTTTTCTGTTTACGTCCTTCAATTTGTCTAATCTGTTTTCTCGCCTGATTCTTTTGCAGCTATGCCGCTTACGGATTGAGAACTTATGTCGGAGCAGTTTTGCTGGTTCGTTCCTTCTTCATTCACAATGCTTGTATTTTTGTTGCTTTAATTCACTTCTATTTCACCAATCTTTTGGTTCTTGCTTATCGATTTCTTTAAGCATCTAGAATTTCTTACTGGCTCTGTTGATCTCATTACTTTATTCCATTTTGGGAGAAATCTTCTCATTGAATCTGAATTCAGGAAGCTTTTCTGCTATAAAATTTATTAATGAACATTTCATCTTGCGATCAGAAACAGTGCTTAGTTGCTGAATTAGTTTTGAACCCGCTCGTTGTTTCTCATATTAGGTTGAACTATTTAAACATCCTCATTTGTTGCTATTGCGATTACGAAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCTAATGAATCAGGTAACTGTGTGCTGTGAATCTATTCTGCAGCTGTGTTTGTGTAACATAGTTAAGCTGATGAATATTTGATCATGGTGGATGATCTCAATTTACAGATCTTGATGGTCTGAGACGAAAGCTGTCAAGCAAGCTATCTGCCATTGGAGCCTCTGATGCATCTGATTGGGAGGTACTGCAGTTTCCAAATCTGTTCATTTGATTTGAATGATTGAATTTGAAGTGCTCTTCTTTTAACGTTTAGATCGGCGAGTGCCTTGGGATGTGGTGGAGGCCTGACTTTGAAACCTTTGATCCACACTTGTCCAGTAATGTAAAAGGCGCCAAGGTAGTCATTGGGACACAGGCTACTTCCTTTAAGCCATGGCTTTTCCTGTAACTCACTCACTACCTGATGCAGGAGTGCACTAAACTCTTCCTGGTCAAGCTGCCGGAGAATCGAGAATTCTTCGTGCCAAAGAACCTCAAGTTGATAGCAGTTCCTTTATGCCAGATTCAAAAAAGCCATGAGGTATCCTTATCAACACACTTTATGGAAGTCCAATTTATCACGTTTGCAAATCTTAGTTTTTCCTTGAAAGATTGAAAGAAAAAGGAAAGACGGTTCAATTTTGTTGGGGAAATTCTTGAAATAAATCTCTATTGGGTTCATAGGGCATTAGGTCGGATTAGGATTTTTTAAAATGGAATAGTAAACTTTTTCAACTTAATCCATCTTTATTAGATATTGATCCTAACCCAACTAACTTGGTTTAGGTGGTTCTAGTTTAGATCATTTATTCTTTTTTTTTTTTTACTTAATTCTTAGAACATTGATTTTGAATTTAGATTTACAACTTAATTTCAATATATTTTTTGAATGAAAAATGAGAATGATGTGAAAATAGTTATGAAACTAAATAAAATTAAAATAAATATAGGTATATAATTGTATCATAAGTAAATTTTTAATGAACTTATTACCGGATCTAAATTGTATGAGTTGGGTTGAATTATTCGGTTTATTTGGGTCATTGATTTTTTTTTAAGCACTCTTAATTGTTGAATTATAGTAAGGTGCACTAGTCAACTACGTGTCAATTCTTTGATCTTGTAGACGTACGGGCCAGTCATATCGAGCATTCCACAGCTGCTTTCTAAGTTCTCCTTCAACATCATTGGAACCTAAGAATATTTATGAGTGCATTATAGAGGCGTGAGGTAAGTTTGTATGAATGTTTGAGAGATCCTTTTTAAAACATGCACATAGACGATGGTGATGGTTTTGTTAAGCATAAAGATAACAGTCATATAGTTCCCACATTGTTACTTCACAACTTGAGTTCTAAGGAATGCTAAAACTAGACGATTGCTTAAATTGCCAATTCTTGACTTTCTTATGCTTTGTTAAATGTGGCAAATATTTATGTGATCCTTATCCCATGAAAATACTTGTCGG

mRNA sequence

TCCACTCTCTGCAACCACACGCCATCTTCAAACATCCAGACCTATATTCTCTACTCCCACTCACTTTCCTCCTCTCTCTCCTCTCCATATTCGATTCTCTCTATCTCTCTCTGTTTTTCTGCAATCGGCCATGGGAGACGACGGACATGTAAATGCTATGGAGGACGATGAAGCTTCTGCCGCCGAGCAACGCGGAGACATCGACATTTACCCTCTCAGAAACTACTTCTTTGGATCCAAAGAGGCTCTGCTTTTCAAGAACGAGGCCTTGGCTGATCGCGTCCAGAGGATGAATTCCAACTATGCCGCTTACGGATTGAGAACTTATGTCGGAGCAGTTTTGCTGGTTGAACTATTTAAACATCCTCATTTGTTGCTATTGCGATTACGAAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCTAATGAATCAGATCTTGATGGTCTGAGACGAAAGCTGTCAAGCAAGCTATCTGCCATTGGAGCCTCTGATGCATCTGATTGGGAGATCGGCGAGTGCCTTGGGATGTGGTGGAGGCCTGACTTTGAAACCTTTGATCCACACTTGTCCAGTAATGTAAAAGGCGCCAAGGAGTGCACTAAACTCTTCCTGGTCAAGCTGCCGGAGAATCGAGAATTCTTCGTGCCAAAGAACCTCAAGTTGATAGCAGTTCCTTTATGCCAGATTCAAAAAAGCCATGAGACGTACGGGCCAGTCATATCGAGCATTCCACAGCTGCTTTCTAAGTTCTCCTTCAACATCATTGGAACCTAAGAATATTTATGAGTGCATTATAGAGGCGTGAGGTAAGTTTGTATGAATGTTTGAGAGATCCTTTTTAAAACATGCACATAGACGATGGTGATGGTTTTGTTAAGCATAAAGATAACAGTCATATAGTTCCCACATTGTTACTTCACAACTTGAGTTCTAAGGAATGCTAAAACTAGACGATTGCTTAAATTGCCAATTCTTGACTTTCTTATGCTTTGTTAAATGTGGCAAATATTTATGTGATCCTTATCCCATGAAAATACTTGTCGG

Coding sequence (CDS)

ATGGGAGACGACGGACATGTAAATGCTATGGAGGACGATGAAGCTTCTGCCGCCGAGCAACGCGGAGACATCGACATTTACCCTCTCAGAAACTACTTCTTTGGATCCAAAGAGGCTCTGCTTTTCAAGAACGAGGCCTTGGCTGATCGCGTCCAGAGGATGAATTCCAACTATGCCGCTTACGGATTGAGAACTTATGTCGGAGCAGTTTTGCTGGTTGAACTATTTAAACATCCTCATTTGTTGCTATTGCGATTACGAAATTCAATTTTCAAGCTTCCTGGTGGTCGCCTAAGGCCTAATGAATCAGATCTTGATGGTCTGAGACGAAAGCTGTCAAGCAAGCTATCTGCCATTGGAGCCTCTGATGCATCTGATTGGGAGATCGGCGAGTGCCTTGGGATGTGGTGGAGGCCTGACTTTGAAACCTTTGATCCACACTTGTCCAGTAATGTAAAAGGCGCCAAGGAGTGCACTAAACTCTTCCTGGTCAAGCTGCCGGAGAATCGAGAATTCTTCGTGCCAAAGAACCTCAAGTTGATAGCAGTTCCTTTATGCCAGATTCAAAAAAGCCATGAGACGTACGGGCCAGTCATATCGAGCATTCCACAGCTGCTTTCTAAGTTCTCCTTCAACATCATTGGAACCTAA

Protein sequence

MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT

Homology

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BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot
Match: Q94AF0 (Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CFIS1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.0e-70
Identity = 132/221 (59.73%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGD------IDIYPLRNYFFGSKEALLFKNEALADRVQRM 60
           MG++     ME+   +   +R D      +D+YPL +Y+FGSKEAL  K+E ++DRV R+
Sbjct: 1   MGEEARALDMEEISDNTT-RRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRL 60

Query: 61  NSNYAAYGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSS 120
            SNYAA+GLRT V AVLLVELFKHPH+LLL+ RNSIFKLPGGRLRP ESD++GL+RKL+S
Sbjct: 61  KSNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLAS 120

Query: 121 KLSAIGASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFF 180
           KLS       S +E+GEC+GMWWRP+FET   P L  N+K  KECTKLFLV+LP +++F 
Sbjct: 121 KLSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFV 180

Query: 181 VPKNLKLIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNII 215
           VPKN KL+AVPLCQ+ ++ +TYGP++S IP+LLSKFSFN++
Sbjct: 181 VPKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMM 220

[Close]

BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot
Match: Q8GXS3 (Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=CFIS2 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-52
Identity = 98/194 (50.52%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 24  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLL 83
           ++ YPL NY FG+KE  L K+ ++ADR+ RM  NY   G+RT V  +LLV+   HPH+LL
Sbjct: 7   VNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHILL 66

Query: 84  LRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDASDWEIGECLGMWWRPDFET 143
           L++ N+  KLPGGRL+P E++ DGL+RKL+SKL    A+   DW +GEC+  WWRP+FET
Sbjct: 67  LQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNFET 126

Query: 144 -FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVISSI 203
              P+   ++   KEC +L++V L E   F VPKNLKL+AVPL ++  + + YGPVIS+I
Sbjct: 127 MMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVISTI 186

Query: 204 PQLLSKFSFNIIGT 217
           PQ LS+F FN+I +
Sbjct: 187 PQQLSRFHFNMISS 200

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BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot
Match: Q7T3C6 (Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955 GN=nudt21 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.2e-42
Identity = 87/191 (45.55%), Postives = 124/191 (64.92%), Query Frame = 0


Query: 24  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLL 83
           I++YPL NY FG+KE L  K+ ++A R QRM   +   G+R  V  VL+V   + PH+LL
Sbjct: 38  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFEKIGMRRTVEGVLIVHEHRLPHVLL 97

Query: 84  LRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDA--SDWEIGECLGMWWRPDF 143
           L+L  + FKLPGG L P E +++GL+R ++     +G  D    DW I +C+G WWRP+F
Sbjct: 98  LQLGTTFFKLPGGELNPGEDEVEGLKRLMT---EILGRQDGVKQDWVIDDCIGNWWRPNF 157

Query: 144 ETFD-PHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVIS 203
           E    P++ +++   KE  KLFLV+L E   F VPKN KL+A PL ++  +   YGP+IS
Sbjct: 158 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 217

Query: 204 SIPQLLSKFSF 212
           S+PQLLS+F+F
Sbjct: 218 SLPQLLSRFNF 225

[Close]

BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot
Match: Q3ZCA2 (Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 GN=NUDT21 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-42
Identity = 87/191 (45.55%), Postives = 124/191 (64.92%), Query Frame = 0


Query: 24  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLL 83
           I++YPL NY FG+KE L  K+ ++A R QRM   +   G+R  V  VL+V   + PH+LL
Sbjct: 37  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLL 96

Query: 84  LRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDA--SDWEIGECLGMWWRPDF 143
           L+L  + FKLPGG L P E +++GL+R ++     +G  D    DW I +C+G WWRP+F
Sbjct: 97  LQLGTTFFKLPGGELNPGEDEVEGLKRLMT---EILGRQDGVLQDWVIDDCIGNWWRPNF 156

Query: 144 ETFD-PHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVIS 203
           E    P++ +++   KE  KLFLV+L E   F VPKN KL+A PL ++  +   YGP+IS
Sbjct: 157 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 216

Query: 204 SIPQLLSKFSF 212
           S+PQLLS+F+F
Sbjct: 217 SLPQLLSRFNF 224

[Close]

BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot
Match: O43809 (Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=9606 GN=NUDT21 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-42
Identity = 87/191 (45.55%), Postives = 124/191 (64.92%), Query Frame = 0


Query: 24  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLL 83
           I++YPL NY FG+KE L  K+ ++A R QRM   +   G+R  V  VL+V   + PH+LL
Sbjct: 37  INLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLL 96

Query: 84  LRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDA--SDWEIGECLGMWWRPDF 143
           L+L  + FKLPGG L P E +++GL+R ++     +G  D    DW I +C+G WWRP+F
Sbjct: 97  LQLGTTFFKLPGGELNPGEDEVEGLKRLMT---EILGRQDGVLQDWVIDDCIGNWWRPNF 156

Query: 144 ETFD-PHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVIS 203
           E    P++ +++   KE  KLFLV+L E   F VPKN KL+A PL ++  +   YGP+IS
Sbjct: 157 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 216

Query: 204 SIPQLLSKFSF 212
           S+PQLLS+F+F
Sbjct: 217 SLPQLLSRFNF 224

[Close]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL
Match: A0A6J1G4C8 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC111450684 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 4.8e-120
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL
Match: A0A6J1KFM8 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111493862 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.2e-116
Identity = 211/216 (97.69%), Postives = 212/216 (98.15%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQR  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRRAIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSI +LPGGRLRPNESDLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIVQLPGGRLRPNESDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLV LPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVMLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL
Match: A0A6J1DN47 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111021937 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.3e-93
Identity = 172/215 (80.00%), Postives = 192/215 (89.30%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGD+GH++AMEDDEA+ AEQ   + IYPL NY+FGSKE LLFKNE LADR+ RM SNYAA
Sbjct: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
            GLRT V AVLLVELFKHPHLLLL++RNSIFKLPGGRLRPNESD+DGL+RKLS KLSA G
Sbjct: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 ASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLK 180
            SDASDWE+GECLGMWWRPDFET   P+LSSNVKGAKECTKLFLVKLPE+R+F VPKNLK
Sbjct: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNII 215
           LIAVPLCQ+ ++ +TYGPVIS +PQLLSKFSFNII
Sbjct: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNII 215

[Close]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL
Match: A0A6J1FHN4 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC111444047 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.9e-93
Identity = 171/217 (78.80%), Postives = 193/217 (88.94%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDG ++AMEDDE++AA+Q   IDIYPL NY+FGSKE L+FK++ LADRV RM SNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRT V AVLLVELFKHPH+LLL++RNSIF LPGGRLRPNESD+DGL+RKLS KLSA G
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 ASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLK 180
           A DASDWE+G+CLG WWRPDFET   PHLS+NVKGAKECTKLFLVKLPENR+F VPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKNLK 180

Query: 181 LIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           LIAVPLCQ+ ++H+ YGPVIS IPQLLSKFSFNII T
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL
Match: A0A0A0LIM0 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G000310 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.2e-92
Identity = 168/217 (77.42%), Postives = 193/217 (88.94%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDG ++A++DD+AS+A Q   IDIYPL NY+FGSKE LLFK+E L+DRV RM SNYAA
Sbjct: 1   MGDDGRLHAIDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           +GLRT V AV+LVELFKHPHLLL ++RNSIFKLPGGR+RPNESD+DGL RKL+ KLSA G
Sbjct: 61  HGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRIRPNESDIDGLTRKLTKKLSANG 120

Query: 121 ASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLK 180
           ASDAS WE+ ECLGMWWRPDFET   P+LS+NVKGAKECTKLFLVKLPE+R+F VPKNLK
Sbjct: 121 ASDASHWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECTKLFLVKLPESRKFVVPKNLK 180

Query: 181 LIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           LIAVPLCQI ++H+TYGP+IS IPQLLSKFSFNIIGT
Sbjct: 181 LIAVPLCQIHENHKTYGPIISGIPQLLSKFSFNIIGT 217

[Close]

BLAST of CmoCh05G014040 vs. NCBI nr
Match: XP_022946691.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita moschata])

HSP 1 Score: 440.3 bits (1131), Expect = 9.8e-120
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. NCBI nr
Match: KAG6599518.1 (Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434.9 bits (1117), Expect = 4.1e-118
Identity = 214/216 (99.07%), Postives = 214/216 (99.07%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQRG IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRGGIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSI KLPGGRLRPNESDLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIVKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. NCBI nr
Match: XP_022999530.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita maxima])

HSP 1 Score: 427.6 bits (1098), Expect = 6.6e-116
Identity = 211/216 (97.69%), Postives = 212/216 (98.15%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQR  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRRAIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSI +LPGGRLRPNESDLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIVQLPGGRLRPNESDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLV LPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVMLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. NCBI nr
Match: XP_023545701.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.1 bits (1063), Expect = 7.5e-112
Identity = 205/216 (94.91%), Postives = 207/216 (95.83%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGDDGHVNAMEDDEASAAEQR  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA
Sbjct: 1   MGDDGHVNAMEDDEASAAEQRRAIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
           YGLRTYVGAVLLVELFKHPHLLLLRLRNSI KLPG   R   +DLDGLRRKLSSKLSAIG
Sbjct: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIVKLPGXHKRSQFTDLDGLRRKLSSKLSAIG 120

Query: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180
           ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL
Sbjct: 121 ASDASDWEIGECLGMWWRPDFETFDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKL 180

Query: 181 IAVPLCQIQKSHETYGPVISSIPQLLSKFSFNIIGT 217
           IAVPLCQIQKSHETYGPVISS+PQLLSKFSFNIIGT
Sbjct: 181 IAVPLCQIQKSHETYGPVISSLPQLLSKFSFNIIGT 216

[Close]

BLAST of CmoCh05G014040 vs. NCBI nr
Match: XP_022154764.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Momordica charantia])

HSP 1 Score: 352.4 bits (903), Expect = 2.7e-93
Identity = 172/215 (80.00%), Postives = 192/215 (89.30%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGDIDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAA 60
           MGD+GH++AMEDDEA+ AEQ   + IYPL NY+FGSKE LLFKNE LADR+ RM SNYAA
Sbjct: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60

Query: 61  YGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIG 120
            GLRT V AVLLVELFKHPHLLLL++RNSIFKLPGGRLRPNESD+DGL+RKLS KLSA G
Sbjct: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 121 ASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLK 180
            SDASDWE+GECLGMWWRPDFET   P+LSSNVKGAKECTKLFLVKLPE+R+F VPKNLK
Sbjct: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 181 LIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNII 215
           LIAVPLCQ+ ++ +TYGPVIS +PQLLSKFSFNII
Sbjct: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNII 215

[Close]

BLAST of CmoCh05G014040 vs. TAIR 10
Match: AT4G29820.1 (homolog of CFIM-25 )

HSP 1 Score: 266.9 bits (681), Expect = 1.4e-71
Identity = 132/221 (59.73%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 1   MGDDGHVNAMEDDEASAAEQRGD------IDIYPLRNYFFGSKEALLFKNEALADRVQRM 60
           MG++     ME+   +   +R D      +D+YPL +Y+FGSKEAL  K+E ++DRV R+
Sbjct: 1   MGEEARALDMEEISDNTT-RRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRL 60

Query: 61  NSNYAAYGLRTYVGAVLLVELFKHPHLLLLRLRNSIFKLPGGRLRPNESDLDGLRRKLSS 120
            SNYAA+GLRT V AVLLVELFKHPH+LLL+ RNSIFKLPGGRLRP ESD++GL+RKL+S
Sbjct: 61  KSNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLAS 120

Query: 121 KLSAIGASDASDWEIGECLGMWWRPDFET-FDPHLSSNVKGAKECTKLFLVKLPENREFF 180
           KLS       S +E+GEC+GMWWRP+FET   P L  N+K  KECTKLFLV+LP +++F 
Sbjct: 121 KLSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFV 180

Query: 181 VPKNLKLIAVPLCQIQKSHETYGPVISSIPQLLSKFSFNII 215
           VPKN KL+AVPLCQ+ ++ +TYGP++S IP+LLSKFSFN++
Sbjct: 181 VPKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMM 220

[Close]

BLAST of CmoCh05G014040 vs. TAIR 10
Match: AT4G25550.1 (Cleavage/polyadenylation specificity factor, 25kDa subunit )

HSP 1 Score: 208.0 bits (528), Expect = 7.6e-54
Identity = 98/194 (50.52%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 24  IDIYPLRNYFFGSKEALLFKNEALADRVQRMNSNYAAYGLRTYVGAVLLVELFKHPHLLL 83
           ++ YPL NY FG+KE  L K+ ++ADR+ RM  NY   G+RT V  +LLV+   HPH+LL
Sbjct: 7   VNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHILL 66

Query: 84  LRLRNSIFKLPGGRLRPNESDLDGLRRKLSSKLSAIGASDASDWEIGECLGMWWRPDFET 143
           L++ N+  KLPGGRL+P E++ DGL+RKL+SKL    A+   DW +GEC+  WWRP+FET
Sbjct: 67  LQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNFET 126

Query: 144 -FDPHLSSNVKGAKECTKLFLVKLPENREFFVPKNLKLIAVPLCQIQKSHETYGPVISSI 203
              P+   ++   KEC +L++V L E   F VPKNLKL+AVPL ++  + + YGPVIS+I
Sbjct: 127 MMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVISTI 186

Query: 204 PQLLSKFSFNIIGT 217
           PQ LS+F FN+I +
Sbjct: 187 PQQLSRFHFNMISS 200

The following BLAST results are available for this feature:

BLAST of CmoCh05G014040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AF0	2.0e-70	59.73	Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GXS3	1.1e-52	50.52	Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q7T3C6	2.2e-42	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955...	[more]
Q3ZCA2	2.9e-42	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 ...	[more]
O43809	2.9e-42	45.55	Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=960...	[more]

BLAST of CmoCh05G014040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4C8	4.8e-120	100.00	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1KFM8	3.2e-116	97.69	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1DN47	1.3e-93	80.00	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1FHN4	2.9e-93	78.80	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0LIM0	3.2e-92	77.42	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G0...	[more]

BLAST of CmoCh05G014040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022946691.1	9.8e-120	100.00	pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita moschata]	[more]
KAG6599518.1	4.1e-118	99.07	Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma su...	[more]
XP_022999530.1	6.6e-116	97.69	pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita maxima]	[more]
XP_023545701.1	7.5e-112	94.91	pre-mRNA cleavage factor Im 25 kDa subunit 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022154764.1	2.7e-93	80.00	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Momordica charantia]	[more]

BLAST of CmoCh05G014040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29820.1	1.4e-71	59.73	homolog of CFIM-25	[more]
AT4G25550.1	7.6e-54	50.52	Cleavage/polyadenylation specificity factor, 25kDa subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PFAM	PF13869	NUDIX_2	coord: 23..209 e-value: 6.1E-62 score: 208.5
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PIRSF	PIRSF017888	CPSF-25	coord: 3..216 e-value: 8.0E-71 score: 235.8
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PANTHER	PTHR13047	PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT	coord: 8..214
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 23..213 e-value: 1.8E-66 score: 225.2
None	No IPR available	PANTHER	PTHR13047:SF1	PRE-MRNA CLEAVAGE FACTOR IM 25 KDA SUBUNIT 1	coord: 8..214
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 62..187 score: 8.847013
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 58..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G014040.1	CmoCh05G014040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006378	mRNA polyadenylation
biological_process	GO:0006457	protein folding
cellular_component	GO:0005832	chaperonin-containing T-complex
cellular_component	GO:0005849	mRNA cleavage factor complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0051082	unfolded protein binding