CmoCh04G012930 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G012930
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMembrane-associated kinase regulator 4
LocationCmo_Chr04: 6550841 .. 6551890 (-)
RNA-Seq ExpressionCmoCh04G012930
SyntenyCmoCh04G012930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTGACATCCCATCTTATGATCCATCAGATGATGACTATATAGACATGGAAATTGGTTCATACACCAACTTTCTCTGCGACTCCAAAAGCTCTCCGCGCCAATCCAGAGAGTTTGAGTTTCAAATGTCTATGAATTCTCGGGGGAGAGAGCCGACGACTTCTCCAGCGGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGGTAGAGAGACTCTTGCAGAAGTCTAGTTCTGATTATGATTTCAGAAAAGACACTTTTGAGGAATTTTTCAGCACTCCGTTAACCACTTCTATCAATACACCTACAGCAACAAGTACTCCTTTTGATTCTTGCAACATTTCTCCTTCCGAGTCCTTTCGGATTAGTAGGGAACTTAACCCAGATGAATATTTTCTAGAGTATTCAGCAGATGCGAGCTATTTGAATGGTGAAAGCTCAAAAAAGTCCTGGACTAAAAAGCTTAAACGTTCTACACTTGGTCTTAAGTTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACCAAAACAGGCTGCTCAGATGAGTCCTGTGCAGTAGCTGCAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCAATGAATGTTCAAAAAAGGGCGCCAAAGTGGAAAAGAGAAAGCCCTTTGGACCAATTTCAAGCTCCATCTCCAATTCCAACAAAGACAAAGGCAATACGGAAAGCAGCAGAAGCAATAGAAGATCATTCTCATTGGTCATTAAGAGGCAACCAAACATCAAACCATCTTCTGCATCATTAAATCCCACTCCCTCTTCATGTCCAAACAATTCGAGCGGGTATTACCTGAAGAGATGTGGCAGTGTGAATTCAGAGATTGAGAACTCAATTCAGGGAGCAATTGCACACTGCAAGCAATCTCAATCTCAGCAACCATTGCGTTCAAGGAAGACAGTTAGTGAAGTTGGAATTTACTTATTATCATCCTCCAGCCTTACAGTTTCTGATGATCAGGAAAAACCAGAACTTTGCAGGGGCTGA

mRNA sequence

ATGGCTTTTGACATCCCATCTTATGATCCATCAGATGATGACTATATAGACATGGAAATTGGTTCATACACCAACTTTCTCTGCGACTCCAAAAGCTCTCCGCGCCAATCCAGAGAGTTTGAGTTTCAAATGTCTATGAATTCTCGGGGGAGAGAGCCGACGACTTCTCCAGCGGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGGTAGAGAGACTCTTGCAGAAGTCTAGTTCTGATTATGATTTCAGAAAAGACACTTTTGAGGAATTTTTCAGCACTCCGTTAACCACTTCTATCAATACACCTACAGCAACAAGTACTCCTTTTGATTCTTGCAACATTTCTCCTTCCGAGTCCTTTCGGATTAGTAGGGAACTTAACCCAGATGAATATTTTCTAGAGTATTCAGCAGATGCGAGCTATTTGAATGGTGAAAGCTCAAAAAAGTCCTGGACTAAAAAGCTTAAACGTTCTACACTTGGTCTTAAGTTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACCAAAACAGGCTGCTCAGATGAGTCCTGTGCAGTAGCTGCAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCAATGAATGTTCAAAAAAGGGCGCCAAAGTGGAAAAGAGAAAGCCCTTTGGACCAATTTCAAGCTCCATCTCCAATTCCAACAAAGACAAAGGCAATACGGAAAGCAGCAGAAGCAATAGAAGATCATTCTCATTGGTCATTAAGAGGCAACCAAACATCAAACCATCTTCTGCATCATTAAATCCCACTCCCTCTTCATGTCCAAACAATTCGAGCGGGTATTACCTGAAGAGATGTGGCAGTGTGAATTCAGAGATTGAGAACTCAATTCAGGGAGCAATTGCACACTGCAAGCAATCTCAATCTCAGCAACCATTGCGTTCAAGGAAGACAGTTAGTGAAGTTGGAATTTACTTATTATCATCCTCCAGCCTTACAGTTTCTGATGATCAGGAAAAACCAGAACTTTGCAGGGGCTGA

Coding sequence (CDS)

ATGGCTTTTGACATCCCATCTTATGATCCATCAGATGATGACTATATAGACATGGAAATTGGTTCATACACCAACTTTCTCTGCGACTCCAAAAGCTCTCCGCGCCAATCCAGAGAGTTTGAGTTTCAAATGTCTATGAATTCTCGGGGGAGAGAGCCGACGACTTCTCCAGCGGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGGTAGAGAGACTCTTGCAGAAGTCTAGTTCTGATTATGATTTCAGAAAAGACACTTTTGAGGAATTTTTCAGCACTCCGTTAACCACTTCTATCAATACACCTACAGCAACAAGTACTCCTTTTGATTCTTGCAACATTTCTCCTTCCGAGTCCTTTCGGATTAGTAGGGAACTTAACCCAGATGAATATTTTCTAGAGTATTCAGCAGATGCGAGCTATTTGAATGGTGAAAGCTCAAAAAAGTCCTGGACTAAAAAGCTTAAACGTTCTACACTTGGTCTTAAGTTGAAAGCTTCCAGGGCTTACATCAAATCTTTGTTTACCAAAACAGGCTGCTCAGATGAGTCCTGTGCAGTAGCTGCAAAGATTGCAGATGAAGGATCAGCTTCAAAAGCCAATGAATGTTCAAAAAAGGGCGCCAAAGTGGAAAAGAGAAAGCCCTTTGGACCAATTTCAAGCTCCATCTCCAATTCCAACAAAGACAAAGGCAATACGGAAAGCAGCAGAAGCAATAGAAGATCATTCTCATTGGTCATTAAGAGGCAACCAAACATCAAACCATCTTCTGCATCATTAAATCCCACTCCCTCTTCATGTCCAAACAATTCGAGCGGGTATTACCTGAAGAGATGTGGCAGTGTGAATTCAGAGATTGAGAACTCAATTCAGGGAGCAATTGCACACTGCAAGCAATCTCAATCTCAGCAACCATTGCGTTCAAGGAAGACAGTTAGTGAAGTTGGAATTTACTTATTATCATCCTCCAGCCTTACAGTTTCTGATGATCAGGAAAAACCAGAACTTTGCAGGGGCTGA

Protein sequence

MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFDSCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAYIKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDKGNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Homology
BLAST of CmoCh04G012930 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-63
Identity = 171/361 (47.37%), Postives = 227/361 (62.88%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMS-MNSRGREPTTSPAD 60
           MA  +   D  ++DYIDME+ S+TN +  + S+    REFEFQMS +     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTNLVRKTLSN-NYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTT-SINTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+++L+    DY F     +EF+STPL T ++ TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILE----DYTFD----DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FDSCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKL---KRSTLGLKLK 180
           F+SC +SP+ES ++S+ELNP++YFLEYS   S    +  KKSWT KL   K+S+LG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYS--DSLEEDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKTGCSDE-SCA-VAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSI 240
           ASRAY++S F KT CSDE SCA  AA++ADE S            +  + KPFG I    
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIK--- 240

Query: 241 SNSNKDKGNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGY----YLKRC 300
           +   K + N   S S+RRSFS+ ++RQ     ++ S         +NS G+    +LKR 
Sbjct: 241 TERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNNKS---------SNSLGFRPLQFLKRS 300

Query: 301 GSVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCR 350
            S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS+S +   DDQE  ++ R
Sbjct: 301 TSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFR 328

BLAST of CmoCh04G012930 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-28
Identity = 129/354 (36.44%), Postives = 174/354 (49.15%), Query Frame = 0

Query: 12  DDDYIDMEI----------GSYTNFLCDSKSSPRQSREFEFQMSMNS-RGREPTTSPADE 71
           DD YIDME+           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 131
           LFY G+LLPLH PPRL+MV++LL  SSS              TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSS--------TAATETPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSES--FRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASR 191
           SC I   E+  F IS EL   + F+E        N      SW+KK+K S++  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNK 251
           AYIK+LF+K  CSD S                        ++  R    P  S +S    
Sbjct: 198 AYIKALFSKQACSDSS------------------------EINPRFKIEP--SKVSRKKN 257

Query: 252 DKGNTESS-RSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEI- 311
              N+E+    +RRSFS VI+R    K S++S   + SS  + SS +     GS++ +  
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSS--SSSSSASSLSSSFSFGSNGSLDLQTL 317

Query: 312 -------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEK 344
                  +NSI+GAI HCKQS + +  +S  T SE+     S +S++   D +K
Sbjct: 318 MRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGDLDK 320

BLAST of CmoCh04G012930 vs. ExPASy Swiss-Prot
Match: Q3E936 (Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 GN=MAKR1 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.5e-06
Identity = 99/347 (28.53%), Postives = 138/347 (39.77%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSMNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVERL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL +V  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDY----------DFRKDTFEEFFSTPLTTSINTPTATSTPFDSCNISPSESFRISRELN 149
           SDY             +D+ E   ST  T S   P     P D C     +S R S   +
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASF--PLLHPPPLDCC-----DSSRPSSVTD 134

Query: 150 PDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAYIK-----SLFTKTGCSD 209
            +++F +                   K K S+ G  L    +  K     +L   +  S 
Sbjct: 135 DEDFFFK-----------------PPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSS 194

Query: 210 ESCAVAAKIADEGSASKANECSKKGAKVEK-------RKPFGPISSSISNSNKDKGN--- 269
            +   AA  + +  +S A E  +K  K  K        K    I +  S+S KD GN   
Sbjct: 195 TATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIR 254

Query: 270 --------------TESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNS-----SG 319
                           S      SFS  + +       +AS   +  S PN+S      G
Sbjct: 255 GTTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGG 314

BLAST of CmoCh04G012930 vs. ExPASy TrEMBL
Match: A0A6J1GXX8 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111458502 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 7.0e-190
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of CmoCh04G012930 vs. ExPASy TrEMBL
Match: A0A6J1JSI7 (probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=LOC111489379 PE=4 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.1e-182
Identity = 341/349 (97.71%), Postives = 342/349 (97.99%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPS DPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSSDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVE+L QKSSSDYDFRKDTFEE FSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLWQKSSSDYDFRKDTFEE-FSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELN DEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNSDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRTY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESS SNRRSFSLVIKRQPNIKPSSASLNPT SSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSSASLNPTSSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 348

BLAST of CmoCh04G012930 vs. ExPASy TrEMBL
Match: A0A5A7V1W4 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00070 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.9e-163
Identity = 311/350 (88.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300
           GNTE+S  NRRSFSLVIKRQP IK SS A L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of CmoCh04G012930 vs. ExPASy TrEMBL
Match: A0A1S3CEW1 (probable membrane-associated kinase regulator 4 OS=Cucumis melo OX=3656 GN=LOC103500173 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.9e-163
Identity = 311/350 (88.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300
           GNTE+S  NRRSFSLVIKRQP IK SS A L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of CmoCh04G012930 vs. ExPASy TrEMBL
Match: A0A0A0KNS4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177670 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.1e-161
Identity = 308/350 (88.00%), Postives = 323/350 (92.29%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRL+M+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLKMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSVNTP--TSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGESSKKSWTKKLK STL LKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGESSKKSWTKKLKYSTLCLKLKASRTY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKS FTK+GCSDESCA AAKIA EGSASK NECSKKGAKVEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIAHEGSASKPNECSKKGAKVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKP-SSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300
           GNTE+S  NRRSFS+VIKRQP IK  SSA L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSMVIKRQPIIKSLSSAPLHPTSSSCPNISSGNYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           IQGAI HCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIEHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of CmoCh04G012930 vs. NCBI nr
Match: XP_022956992.1 (probable membrane-associated kinase regulator 4 [Cucurbita moschata])

HSP 1 Score: 672.9 bits (1735), Expect = 1.5e-189
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of CmoCh04G012930 vs. NCBI nr
Match: KAG6601024.1 (putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031828.1 putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 670.6 bits (1729), Expect = 7.2e-189
Identity = 348/349 (99.71%), Postives = 348/349 (99.71%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESS SNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of CmoCh04G012930 vs. NCBI nr
Match: XP_023527329.1 (probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666.4 bits (1718), Expect = 1.4e-187
Identity = 346/349 (99.14%), Postives = 347/349 (99.43%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTK+LKRSTLGLKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKRLKRSTLGLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESS SNRRSFSLVIKRQPNIKPSSASLNPT SSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSSASLNPTSSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of CmoCh04G012930 vs. NCBI nr
Match: XP_022993342.1 (probable membrane-associated kinase regulator 4 [Cucurbita maxima])

HSP 1 Score: 649.0 bits (1673), Expect = 2.2e-182
Identity = 341/349 (97.71%), Postives = 342/349 (97.99%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MAFDIPS DPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE
Sbjct: 1   MAFDIPSSDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQMVE+L QKSSSDYDFRKDTFEE FSTPLTTSINTPTATSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLWQKSSSDYDFRKDTFEE-FSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELN DEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNSDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRTY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEIENSI 300
           GNTESS SNRRSFSLVIKRQPNIKPSSASLNPT SSCPNNSSGYYLKRCGSVNSEIENSI
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSSASLNPTSSSCPNNSSGYYLKRCGSVNSEIENSI 300

Query: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG
Sbjct: 301 QGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 348

BLAST of CmoCh04G012930 vs. NCBI nr
Match: XP_008461610.1 (PREDICTED: probable membrane-associated kinase regulator 4 [Cucumis melo] >KAA0062212.1 putative membrane-associated kinase regulator 4 [Cucumis melo var. makuwa])

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-163
Identity = 311/350 (88.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180

Query: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240

Query: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300
           GNTE+S  NRRSFSLVIKRQP IK SS A L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 350
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of CmoCh04G012930 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 171/361 (47.37%), Postives = 227/361 (62.88%), Query Frame = 0

Query: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMS-MNSRGREPTTSPAD 60
           MA  +   D  ++DYIDME+ S+TN +  + S+    REFEFQMS +     + TTSPAD
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTNLVRKTLSN-NYPREFEFQMSHLCPLEIDKTTSPAD 60

Query: 61  ELFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTT-SINTPTATSTP 120
           ELFY GKLLPLH PPRLQMV+++L+    DY F     +EF+STPL T ++ TP  ++TP
Sbjct: 61  ELFYKGKLLPLHLPPRLQMVQKILE----DYTFD----DEFYSTPLATGTVTTPVTSNTP 120

Query: 121 FDSCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKL---KRSTLGLKLK 180
           F+SC +SP+ES ++S+ELNP++YFLEYS   S    +  KKSWT KL   K+S+LG K+K
Sbjct: 121 FESCTVSPAESCQVSKELNPEDYFLEYS--DSLEEDDEKKKSWTTKLRLMKQSSLGTKIK 180

Query: 181 ASRAYIKSLFTKTGCSDE-SCA-VAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSI 240
           ASRAY++S F KT CSDE SCA  AA++ADE S            +  + KPFG I    
Sbjct: 181 ASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIK--- 240

Query: 241 SNSNKDKGNTESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGY----YLKRC 300
           +   K + N   S S+RRSFS+ ++RQ     ++ S         +NS G+    +LKR 
Sbjct: 241 TERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNNKS---------SNSLGFRPLQFLKRS 300

Query: 301 GSVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCR 350
            S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS+S +   DDQE  ++ R
Sbjct: 301 TSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFR 328

BLAST of CmoCh04G012930 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 128.6 bits (322), Expect = 9.5e-30
Identity = 129/354 (36.44%), Postives = 174/354 (49.15%), Query Frame = 0

Query: 12  DDDYIDMEI----------GSYTNFLCDSKSSPRQSREFEFQMSMNS-RGREPTTSPADE 71
           DD YIDME+           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 131
           LFY G+LLPLH PPRL+MV++LL  SSS              TP++        +   F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSS--------TAATETPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSES--FRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASR 191
           SC I   E+  F IS EL   + F+E        N      SW+KK+K S++  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNK 251
           AYIK+LF+K  CSD S                        ++  R    P  S +S    
Sbjct: 198 AYIKALFSKQACSDSS------------------------EINPRFKIEP--SKVSRKKN 257

Query: 252 DKGNTESS-RSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNSSGYYLKRCGSVNSEI- 311
              N+E+    +RRSFS VI+R    K S++S   + SS  + SS +     GS++ +  
Sbjct: 258 PFVNSENPLLIHRRSFSGVIQRHSQAKCSTSS--SSSSSASSLSSSFSFGSNGSLDLQTL 317

Query: 312 -------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEK 344
                  +NSI+GAI HCKQS + +  +S  T SE+     S +S++   D +K
Sbjct: 318 MRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESEL---CSSRTSVSTCGDLDK 320

BLAST of CmoCh04G012930 vs. TAIR 10
Match: AT5G26230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 53.5 bits (127), Expect = 3.9e-07
Identity = 99/347 (28.53%), Postives = 138/347 (39.77%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSMNSRGREPTTSPADELFYNGKLLPLHHPPRLQMVERL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL +V  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDY----------DFRKDTFEEFFSTPLTTSINTPTATSTPFDSCNISPSESFRISRELN 149
           SDY             +D+ E   ST  T S   P     P D C     +S R S   +
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASF--PLLHPPPLDCC-----DSSRPSSVTD 134

Query: 150 PDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAYIK-----SLFTKTGCSD 209
            +++F +                   K K S+ G  L    +  K     +L   +  S 
Sbjct: 135 DEDFFFK-----------------PPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSS 194

Query: 210 ESCAVAAKIADEGSASKANECSKKGAKVEK-------RKPFGPISSSISNSNKDKGN--- 269
            +   AA  + +  +S A E  +K  K  K        K    I +  S+S KD GN   
Sbjct: 195 TATTAAAPSSVKRMSSTAKEVIRKYMKKVKPLYEKLSPKQSSNIKTESSSSLKDSGNNIR 254

Query: 270 --------------TESSRSNRRSFSLVIKRQPNIKPSSASLNPTPSSCPNNS-----SG 319
                           S      SFS  + +       +AS   +  S PN+S      G
Sbjct: 255 GTTTVTTVTAAPTVVSSGGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGG 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O806242.2e-6347.37Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZUS81.3e-2836.44Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q3E9365.5e-0628.53Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GXX87.0e-190100.00probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JSI71.1e-18297.71probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5A7V1W41.9e-16388.86Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3CEW11.9e-16388.86probable membrane-associated kinase regulator 4 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KNS41.1e-16188.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177670 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022956992.11.5e-189100.00probable membrane-associated kinase regulator 4 [Cucurbita moschata][more]
KAG6601024.17.2e-18999.71putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma... [more]
XP_023527329.11.4e-18799.14probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo][more]
XP_022993342.12.2e-18297.71probable membrane-associated kinase regulator 4 [Cucurbita maxima][more]
XP_008461610.14.0e-16388.86PREDICTED: probable membrane-associated kinase regulator 4 [Cucumis melo] >KAA00... [more]
Match NameE-valueIdentityDescription
AT2G39370.11.6e-6447.37unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G37380.19.5e-3036.44unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G26230.13.9e-0728.53unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 228..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..278
NoneNo IPR availablePANTHERPTHR33312:SF21MEMBRANE-ASSOCIATED KINASE REGULATOR 3-RELATEDcoord: 7..348
IPR039620BKI1/Probable membrane-associated kinase regulator 1/3/4PANTHERPTHR33312MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATEDcoord: 7..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G012930.1CmoCh04G012930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity
molecular_function GO:0019210 kinase inhibitor activity