CmoCh03G012020 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G012020
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCarbonic anhydrase
LocationCmo_Chr03: 9373663 .. 9376659 (+)
RNA-Seq ExpressionCmoCh03G012020
SyntenyCmoCh03G012020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCACTGCTCTCCGTCTCTCTCTCTCGTTGTTTATACATTATATATTGTTCATAATCTGAGAGAATTGGCCGAATTTCGACTCCGATTCCATTGCTTTGTCTTGCAAATCCGTTTCCTCGATTCAATTTTCGCTCTAGAAGCCATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCCCAAGAAGTTGGTAAGCTCTTCACCGTTACTGATATATCTCTGTCTATGTTGTTTTGATATTCCCTTTTCCTTTTTCTATCTGCGTTTTGCCTTCCATTTAAGCTATTATGTTTCGGTCTCGATTTTTTTATTTTTTATTCTATCAATTGGTTTTGAGTCGAAGAGGACTGCTCTCGTCTGAATCGTTTGGCGATTCATTTACTTACTCGTGTAAAAATCGGTGTTAGATCTTGAGATGGAAGCTATAGATTGTTTGGCTCGAATATTTACATCGGTAATTCGAAATAGTTGCAGTAAACTCTTGACTGGAAGTCTGCAACATTGTTTTGATTTCTCCCATCTCCATTTTCTTGTTGTTTTCAGATCATATGTTAAGGTTTCTATTTCAATTTCTGATTCTACTGATTAGTTTTTCGACGTACCGCTCTCATCTGAATCGTTTTATGTGAACAATTTTTCTTTCATTTAATCGTGCAAGTCTGCATCATTGTTTTGATTTCTCCCACCTCCATTCTCTTGTTGTGTTTAGACCGTACGTTTAGGTTTCTATGTCCATTTCTGATTCTACTGACTAGTTATTGAATCGACGTACCGCTCTCATCTGAATCGTTTTATGTGAATAACTTCTCATTCCTTTAATCAAAATTAATTGTAAACTCTTGAGATGGAAACTAGAGTTTTGGCTCAAATGCTTACTTCGGTATTTCAAAATCATTGGGTTGCAGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTCATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGTCAGAGAGTGAAGATTGTTCCGAAGCTTGGAGTCCTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTGTAAGAAATTCAACAGATTCCTTCTCTCTTCATCAAATTTTGTGGTTTCTGATTTCTCTATTTTCTTTCGTTTAATGTGCCTGTGGATGTAGATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATCTCTTTTGCATTAAAAAGATGTTACAAACCCATCTTCTTTGACCCTGAAATTAATAGAATATTTTAATTATTGTATCAAGATATAATTATTCTTTTGAATTATCTAATGATGATTCTAAATTGTTGATTAACATTGATAACCCATCAGGTATGACATGGAACTCCACATGTTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAACTGGCAAACCAGACCCTTTTCTCGCACAGGTAAACCACCTCCAAACACAACAAAACAACCCAATTCAAATGGCTCGATTATAAGCTTGAATAGGTCCAAGTGATTTCTGAGTTTTTTTTGTGGTCTAGTTCATGATTTTCCTAAAATTGATTTGTGAGAATTCGAATCAGTACAAATTATTGATGAAAAGGATAACATTTTACCTGTTATGAGTAATAGTTAATTATGCATGTGTCTTTTAATCTGATTTTACCGTATTCTTTTGAGTTCTCTTCAGATCACAAACGTTTTCTTGAAGCAAAGATTAGTTCAAATCTTAGAGCATTGTATTTAATCTCCTCAAATGTACAATTTTGAATAATACCTGTAAAAATTGGTCAATAAATTCCGAACTTACAACTATTTTGTTTCTTAACGGTTTACTTGTAAATGAAGTATTCAAACAATCCCAACCAACCAATCCAATCCCAATACTGTGGCTTAGATTAAGGTCATTTGATTCAAACAAATTTATAATCCAAATAACACACGGCAACCATTTCATAAAAGCTCATTCATTAACAAAAACTTATTGTTTCTTGATGTTCAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAATAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGGTGAGGAAGAGTTTTTTTCTTAACCATTTGATTATGAACATCGAAAGTAAATCTTAAAACGGGTCGATAGAAATTCCCTTATTAACTGTGTCCAAAATGAATGAAATATCATAGGCGAGTTTAGCATATATCAAATGGATTAGAATGAATAAGGAACAAGTTTCCGATTCTTCGAATCGAAAACTTTGAACTTAGCTCTAGCCATTTCGAATTTGTTACAATATTGCCTACATGAATAGCTCAATCTTATCAGAGTCTTTGAATGGTAATGCAACAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATGTAAGAAACTCAATTACTTTTACCACTAAATTCTGACAAATTTGTTGCTTCAAATGTTTTTGAGTACATATTTTCGTAAGTGCAGTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCACATCGTATCGACCTCTCCAAGATCTTGAACCTGATCACATGGACACCATCTTCATTGAATGTCAAGGTCCCTGTTTTATAATATTTTGGTGAAAAAAATATATAAATTTATCACTATCCTTCTTATAAAAAATATTATTATCCATCATCAATGATTTTGTTCTTCATGATAATCCC

mRNA sequence

CCCACTGCTCTCCGTCTCTCTCTCTCGTTGTTTATACATTATATATTGTTCATAATCTGAGAGAATTGGCCGAATTTCGACTCCGATTCCATTGCTTTGTCTTGCAAATCCGTTTCCTCGATTCAATTTTCGCTCTAGAAGCCATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCCCAAGAAGTTGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTCATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGTCAGAGAGTGAAGATTGTTCCGAAGCTTGGAGTCCTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATGACATGGAACTCCACATGTTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAACTGGCAAACCAGACCCTTTTCTCGCACAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAATAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCACATCGTATCGACCTCTCCAAGATCTTGAACCTGATCACATGGACACCATCTTCATTGAATGTCAAGGTCCCTGTTTTATAATATTTTGGTGAAAAAAATATATAAATTTATCACTATCCTTCTTATAAAAAATATTATTATCCATCATCAATGATTTTGTTCTTCATGATAATCCC

Coding sequence (CDS)

ATGAAAATTGGGACTACACTCATCGTTTTCTTCTCGCTTCTGCTGTTTTCCCTACATTTGTCTTCAATCTCGGCCCAAGAAGTTGAGGATGAGAGGGAATTTGATTACAGAGAAGGGAGTGAGAAAGGGCCAAGACATTGGGGAAAGATAAAGAAAGAGTGGGAAGCTTGTCATAATGGGGTTTTGCAATCTCCCATAGACTTATCCAGTCAGAGAGTGAAGATTGTTCCGAAGCTTGGAGTCCTGAAGAGAAGCTACAAACCTTGCAATGCAACGGTCAAAAATAGGGGCCATGACATTTCTATATATTGGGTTGGCAAGGCAGGATCAATCAAGGTAAACGGCGTTGTTTATGATCTTCAACAAGCGCATTGGCACTCACCTTCAGAGCATTCCATCAATGGAAGAAGGTATGACATGGAACTCCACATGTTTCACCAAAGTTCTGATCCCAATGGGAAGAACAAGATTGCTGTTGTTGGTCAACTCTATACAACTGGCAAACCAGACCCTTTTCTCGCACAGTTCAGTAAGCAAATAAGCTACATGATTGATCGAGAGCATGAAATGGGGATTGGAGAGATAGATCCTGCAGATATCAAGTTTGGTGGTAATAAGTACTACAAATACCTTGGTTCTCTCACTGTTCCTCCCTGTAGTGAAGGTGTAATTTGGGTCATAAACAAGAAGATAAGAACAGTTTCAAAAGAGCAAGTGCGACTTCTGAGAGAAGCTGTTCATGATTATGCAGAGCGCAATGCAAGGCCATTGCAGCCGCTCAATAACCGGGAAGTCCACATCGTATCGACCTCTCCAAGATCTTGA

Protein sequence

MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQISYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLREAVHDYAERNARPLQPLNNREVHIVSTSPRS
Homology
BLAST of CmoCh03G012020 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 3.7e-74
Identity = 136/263 (51.71%), Postives = 182/263 (69.20%), Query Frame = 0

Query: 8   IVFFSLL-LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSPI 67
           +  FS+  + S+  ++ S  EVEDEREF+Y++  EKGP  WG++K EWE C  G +QSPI
Sbjct: 13  VALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPI 72

Query: 68  DLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWH 127
           DL ++RV IV  LG L R Y P NAT+KNRGHDI + +   AG+IK+NG  Y+LQQ HWH
Sbjct: 73  DLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWH 132

Query: 128 SPSEHSINGRRYDMELHMFHQSSDPNGKN-KIAVVGQLYTTGKPDPFLAQFSKQISYMID 187
           SPSEH+INGRR+ +ELHM H+     G+N ++AVV  LY  G+ D F+    K++  + +
Sbjct: 133 SPSEHTINGRRFALELHMVHE-----GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAE 192

Query: 188 REH-EMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLRE 247
            E  E  +G IDP  IK G  KYY+Y GSLT PPC++ V W + +K+RTV+++QV+LLR 
Sbjct: 193 MEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRV 252

Query: 248 AVHDYAERNARPLQPLNNREVHI 268
           AVHD A  NARP+QP N R VH+
Sbjct: 253 AVHDDANSNARPVQPTNKRIVHL 270

BLAST of CmoCh03G012020 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.5e-64
Identity = 122/263 (46.39%), Postives = 176/263 (66.92%), Query Frame = 0

Query: 6   TLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSP 65
           T ++F S L  S    + S  EV+DE EF Y E SE GP +WG I+ +W+ C +G LQSP
Sbjct: 7   TKMLFISFLFLSSVFLARSG-EVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSP 66

Query: 66  IDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHW 125
           ID+   R ++V  L +L++ YKP NAT+ NRGHDI +  +   G +K+N   Y L+Q HW
Sbjct: 67  IDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQLHW 126

Query: 126 HSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGK-PDPFLAQFSKQISYMI 185
           H+PSEH+ING R+++E H+ H+S++     K  V+G +Y  G  PDPFL+     +    
Sbjct: 127 HTPSEHTINGERFNLEAHLVHESNN----GKFVVIGIVYEIGLWPDPFLSMIENDLKVPA 186

Query: 186 DREH-EMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLR 245
           +++  E GIG IDP  IK  G KY++Y+GSLT PPC+EGV+W+I++K++TV++ Q++LL+
Sbjct: 187 NKKGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQ 246

Query: 246 EAVHDYAERNARPLQPLNNREVH 267
           EAVHD  E NARP QP N R ++
Sbjct: 247 EAVHDGFETNARPTQPENERYIN 262

BLAST of CmoCh03G012020 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 244.2 bits (622), Expect = 1.7e-63
Identity = 120/244 (49.18%), Postives = 155/244 (63.52%), Query Frame = 0

Query: 27  EVEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSPIDLSSQRVKIVPKLGVLKRSY 86
           EVEDE EF Y     KGP  WG +  EW+ C  G +QSPIDL  + V +  K G+L+  Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 87  KPCNATVKNRGHDISIYWVG--KAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHM 146
            P N T+KNRGHDI + + G  K   + + G  Y LQQ HWHSPSEH+ING+R+ +E H+
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFALEEHL 252

Query: 147 FHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQISYMID-REHEMGIGEIDPADIKFG 206
            H+S D     + AVV  LY  G  DPFL    KQ+  + D    E  +G IDP  + F 
Sbjct: 253 VHESKD----KRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFE 312

Query: 207 GNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLREAVHDYAERNARPLQPLNNR 266
              YY+Y GSLT PPCSE VIW ++K+IRTVS +QV+LLR AVHD ++ NARPLQ +N R
Sbjct: 313 SKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKR 372

Query: 267 EVHI 268
           +V++
Sbjct: 373 KVYL 372

BLAST of CmoCh03G012020 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.6e-61
Identity = 130/270 (48.15%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQ--EVEDEREFDYREGSEKGPRHWGKIKKEWEACH 60
           MKI +   VFF L+  S+ + S S    EVEDE +F+Y +  EKGP +WG++K EW  C 
Sbjct: 1   MKIPSIGYVFF-LIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCG 60

Query: 61  NGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISI-YWVGKAG-SIKVNGV 120
            G +QSPIDL+ +RV I   LG L+  Y P NAT+KNRGHDI + +  G AG  I +NG 
Sbjct: 61  KGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGT 120

Query: 121 VYDLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQF 180
            Y LQQ HWHSPSEH++NG+R+ +E HM HQS D  G+N  AVV   Y  GKPD FL   
Sbjct: 121 EYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKD--GRN--AVVAFFYKLGKPDYFLLTL 180

Query: 181 SKQISYMID-REHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVS 240
            + +  + D  E +  +  + P    F    YY+++GSLT PPCSE VIW I+K++RTV+
Sbjct: 181 ERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVT 240

Query: 241 KEQVRLLREAVHDYAERNARPLQPLNNREV 266
            +Q+ +LR  VHD +  NARPLQ  N R V
Sbjct: 241 LKQLIMLRVTVHDQSNSNARPLQRKNERPV 265

BLAST of CmoCh03G012020 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.6e-61
Identity = 117/267 (43.82%), Postives = 165/267 (61.80%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSIS--AQEVEDEREFDYREGSEKGPRHWGKIKKEWEACH 60
           M      I F ++    L   +IS    EV+DE  F Y + +EKGP  WGKI   W+ C+
Sbjct: 1   MDTNAKTIFFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCN 60

Query: 61  NGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVY 120
            G  QSPIDL+++RV ++       R YKP  A + NRGHDI + W G AG + +    +
Sbjct: 61  TGRYQSPIDLTNERVSLIHD-QAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDF 120

Query: 121 DLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSK 180
           +L Q HWHSPSEH++NG RYD+ELHM H S+    + + AV+G LY  G+P+ FL +   
Sbjct: 121 NLVQCHWHSPSEHTVNGTRYDLELHMVHTSA----RGRTAVIGVLYKLGEPNEFLTKLLN 180

Query: 181 QISYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQ 240
            I  + ++  E+ +G IDP +I+F   K+Y+Y+GSLTVPPC+EGVIW + K++ T+S EQ
Sbjct: 181 GIKAVGNK--EINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQ 240

Query: 241 VRLLREAVHDYAERNARPLQPLNNREV 266
           +  LR+AV D  E N+RP+Q    R V
Sbjct: 241 ITALRQAVDDGFETNSRPVQDSKGRSV 260

BLAST of CmoCh03G012020 vs. ExPASy TrEMBL
Match: A0A6J1EJ09 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111433054 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 8.1e-157
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of CmoCh03G012020 vs. ExPASy TrEMBL
Match: A0A6J1INE0 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111478003 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.5e-139
Identity = 245/271 (90.41%), Postives = 258/271 (95.20%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKI TTLIVFFSLLLFSLHLSSISAQEVEDE EFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 72  MKIETTLIVFFSLLLFSLHLSSISAQEVEDESEFDYREGSEKGPRHWGKIKKEWEACNNG 131

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSS+RVKIVPK G LKRSYKPCNAT+KNRGHDIS+YWVGKAGSIKVNGVVYDL
Sbjct: 132 VLQSPIDLSSERVKIVPKFGDLKRSYKPCNATIKNRGHDISVYWVGKAGSIKVNGVVYDL 191

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHM HQSS+PN KNKIAVVGQLYT G+P+PFLAQFSKQI
Sbjct: 192 QQAHWHSPSEHSINGRRYDMELHMVHQSSNPNVKNKIAVVGQLYTIGEPEPFLAQFSKQI 251

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           S MIDREHE+GIGEIDPADIKFGG KYYKYLGSLTVPPC+EGVIW INKKIRTVS EQV+
Sbjct: 252 SSMIDREHEVGIGEIDPADIKFGGKKYYKYLGSLTVPPCTEGVIWAINKKIRTVSHEQVQ 311

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTS 272
           LLREAVHDYA+RN RPLQ LNNREV+I+STS
Sbjct: 312 LLREAVHDYADRNVRPLQRLNNREVNILSTS 342

BLAST of CmoCh03G012020 vs. ExPASy TrEMBL
Match: A0A0A0KKA6 (Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_6G446560 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.7e-120
Identity = 207/265 (78.11%), Postives = 237/265 (89.43%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           M+  +T IVFF +LL+ +H SSISAQEVEDEREFDY EGS+KGP HWG+IKKEWEAC+NG
Sbjct: 1   MQFCSTPIVFFFILLYFIHSSSISAQEVEDEREFDYAEGSQKGPGHWGEIKKEWEACNNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
            LQSPIDLSSQRVKIVP+LG LKRSY PCNATVKNRGHDIS+YW GK GSI++NG VYDL
Sbjct: 61  DLQSPIDLSSQRVKIVPQLGELKRSYYPCNATVKNRGHDISVYWYGKPGSIEINGDVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQ+HWHSPSEHS+NGRRYD+ELHM HQSSDP  KNKIAVVGQLYT G+PDPF+ Q S++I
Sbjct: 121 QQSHWHSPSEHSVNGRRYDLELHMVHQSSDPTVKNKIAVVGQLYTIGQPDPFITQLSREI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
             M+DR+HE  +G ++PADIKFGG KYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQVR
Sbjct: 181 LGMVDRKHEKKVGVMNPADIKFGGKKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREV 266
           LLREAVHDYAE NARPLQPLN+RE+
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREI 265

BLAST of CmoCh03G012020 vs. ExPASy TrEMBL
Match: A0A6J1CS77 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111013713 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.7e-120
Identity = 210/267 (78.65%), Postives = 235/267 (88.01%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKI T   VFFS+LLF LH SSISAQEVE+EREFDY EGS+KGP HWGK+KKEWEAC+NG
Sbjct: 1   MKIRTKHFVFFSILLFFLHSSSISAQEVENEREFDYIEGSDKGPGHWGKLKKEWEACNNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
            LQSPIDL+SQRVKI+PKLG L RSY PCNAT+KNRGHDISIYWVG AGSI VNGVVYDL
Sbjct: 61  DLQSPIDLTSQRVKIIPKLGELNRSYYPCNATIKNRGHDISIYWVGNAGSILVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQ+HWHSPSEHSING RYD+ELHM H SSDPN KNKIAVVGQLY  G PDPFL+Q  + I
Sbjct: 121 QQSHWHSPSEHSINGVRYDLELHMVHLSSDPNVKNKIAVVGQLYKIGPPDPFLSQLGRDI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           S MID++HE  +G ++PADIKFGG KYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQV+
Sbjct: 181 SGMIDQKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVK 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHI 268
           LLREAVHDYAE NARPLQPLN+R +H+
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSRHIHL 267

BLAST of CmoCh03G012020 vs. ExPASy TrEMBL
Match: A0A1S3CIK8 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103500831 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.0e-119
Identity = 208/274 (75.91%), Postives = 240/274 (87.59%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MK  T  I+FF +LLF LH SSISAQEVEDER+FDY E S+KGP HWG+IKKEWEAC+NG
Sbjct: 1   MKFCTKPILFFFILLFFLHSSSISAQEVEDERDFDYVEWSQKGPGHWGEIKKEWEACNNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
            LQSPIDLSS+RVKIVP+LG LKR+Y PCNATVKNRGHDIS+YW GK GSI++NGVVYDL
Sbjct: 61  DLQSPIDLSSERVKIVPQLGELKRNYYPCNATVKNRGHDISVYWYGKPGSIEINGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQ+HWHSPSEHSINGRRYD+ELHM HQSSDP  KNKIAVVGQLYT G+PDPF+ Q S++I
Sbjct: 121 QQSHWHSPSEHSINGRRYDLELHMVHQSSDPTVKNKIAVVGQLYTIGQPDPFITQLSREI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
             M+DR+HE  +G ++PADIKFGG KYY+YLGSLTVPPC+EGVIW +NKKIRTVS+EQVR
Sbjct: 181 LGMVDRKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSREQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAE NARPLQPLN+RE+ +   + R+
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREIGLYRPTQRT 274

BLAST of CmoCh03G012020 vs. NCBI nr
Match: XP_022925725.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 562.8 bits (1449), Expect = 1.7e-156
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of CmoCh03G012020 vs. NCBI nr
Match: KAG6604394.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034544.1 Alpha carbonic anhydrase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 557.8 bits (1436), Expect = 5.4e-155
Identity = 271/274 (98.91%), Postives = 272/274 (99.27%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGS+KVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSVKVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHM HQSSDPNGKNKIAVVGQLYTTGKPDPFLAQF KQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFCKQI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 274

BLAST of CmoCh03G012020 vs. NCBI nr
Match: XP_023544237.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 539.7 bits (1389), Expect = 1.5e-149
Identity = 263/274 (95.99%), Postives = 267/274 (97.45%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACNNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSSQRVKIVPK G +KRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL
Sbjct: 61  VLQSPIDLSSQRVKIVPKFGDVKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHM HQSSDPNGKNKIAVVGQLYT GKPDPFLAQFSKQI
Sbjct: 121 QQAHWHSPSEHSINGRRYDMELHMVHQSSDPNGKNKIAVVGQLYTIGKPDPFLAQFSKQI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           SYMIDREHEMGIGEIDPADIKFGG KYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR
Sbjct: 181 SYMIDREHEMGIGEIDPADIKFGGKKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAERNARPLQPLNNREV ++STS RS
Sbjct: 241 LLREAVHDYAERNARPLQPLNNREVQVLSTSQRS 274

BLAST of CmoCh03G012020 vs. NCBI nr
Match: XP_022977815.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 505.4 bits (1300), Expect = 3.2e-139
Identity = 245/271 (90.41%), Postives = 258/271 (95.20%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKI TTLIVFFSLLLFSLHLSSISAQEVEDE EFDYREGSEKGPRHWGKIKKEWEAC+NG
Sbjct: 72  MKIETTLIVFFSLLLFSLHLSSISAQEVEDESEFDYREGSEKGPRHWGKIKKEWEACNNG 131

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
           VLQSPIDLSS+RVKIVPK G LKRSYKPCNAT+KNRGHDIS+YWVGKAGSIKVNGVVYDL
Sbjct: 132 VLQSPIDLSSERVKIVPKFGDLKRSYKPCNATIKNRGHDISVYWVGKAGSIKVNGVVYDL 191

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQAHWHSPSEHSINGRRYDMELHM HQSS+PN KNKIAVVGQLYT G+P+PFLAQFSKQI
Sbjct: 192 QQAHWHSPSEHSINGRRYDMELHMVHQSSNPNVKNKIAVVGQLYTIGEPEPFLAQFSKQI 251

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           S MIDREHE+GIGEIDPADIKFGG KYYKYLGSLTVPPC+EGVIW INKKIRTVS EQV+
Sbjct: 252 SSMIDREHEVGIGEIDPADIKFGGKKYYKYLGSLTVPPCTEGVIWAINKKIRTVSHEQVQ 311

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTS 272
           LLREAVHDYA+RN RPLQ LNNREV+I+STS
Sbjct: 312 LLREAVHDYADRNVRPLQRLNNREVNILSTS 342

BLAST of CmoCh03G012020 vs. NCBI nr
Match: XP_038881410.1 (alpha carbonic anhydrase 7-like [Benincasa hispida])

HSP 1 Score: 448.7 bits (1153), Expect = 3.5e-122
Identity = 215/274 (78.47%), Postives = 241/274 (87.96%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNG 60
           MKI T  IVF S+LLF LH S IS+QEVEDEREFDY EGSEKGP HWGK+KKEWEAC+NG
Sbjct: 1   MKIWTKPIVFLSILLFFLHSSLISSQEVEDEREFDYMEGSEKGPGHWGKLKKEWEACNNG 60

Query: 61  VLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDL 120
            LQSPIDLSS RVKI+PKLG LKRSY PCNATVKNRGHDISIYWVGK GSI+VNGVVYDL
Sbjct: 61  DLQSPIDLSSLRVKIIPKLGELKRSYYPCNATVKNRGHDISIYWVGKPGSIQVNGVVYDL 120

Query: 121 QQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQI 180
           QQ+HWHSPSEHSINGRRYD+ELHM H SSDP  KNKIAVVGQLYT G+PDPFL+Q S++I
Sbjct: 121 QQSHWHSPSEHSINGRRYDLELHMVHLSSDPTVKNKIAVVGQLYTIGQPDPFLSQLSREI 180

Query: 181 SYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVR 240
           S M+DR+HE  +G ++PADIKFGG KYY+YLGSLTVPPC+EGVIW +NKKIRTVS EQV+
Sbjct: 181 SSMVDRKHEKKVGVMNPADIKFGGRKYYRYLGSLTVPPCTEGVIWTMNKKIRTVSMEQVK 240

Query: 241 LLREAVHDYAERNARPLQPLNNREVHIVSTSPRS 275
           LLREAVHDYAE NARPLQPLN+RE+ +   + R+
Sbjct: 241 LLREAVHDYAEFNARPLQPLNSREISLYRPTQRT 274

BLAST of CmoCh03G012020 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 279.6 bits (714), Expect = 2.6e-75
Identity = 136/263 (51.71%), Postives = 182/263 (69.20%), Query Frame = 0

Query: 8   IVFFSLL-LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSPI 67
           +  FS+  + S+  ++ S  EVEDEREF+Y++  EKGP  WG++K EWE C  G +QSPI
Sbjct: 13  VALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPI 72

Query: 68  DLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVYDLQQAHWH 127
           DL ++RV IV  LG L R Y P NAT+KNRGHDI + +   AG+IK+NG  Y+LQQ HWH
Sbjct: 73  DLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWH 132

Query: 128 SPSEHSINGRRYDMELHMFHQSSDPNGKN-KIAVVGQLYTTGKPDPFLAQFSKQISYMID 187
           SPSEH+INGRR+ +ELHM H+     G+N ++AVV  LY  G+ D F+    K++  + +
Sbjct: 133 SPSEHTINGRRFALELHMVHE-----GRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAE 192

Query: 188 REH-EMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQVRLLRE 247
            E  E  +G IDP  IK G  KYY+Y GSLT PPC++ V W + +K+RTV+++QV+LLR 
Sbjct: 193 MEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRV 252

Query: 248 AVHDYAERNARPLQPLNNREVHI 268
           AVHD A  NARP+QP N R VH+
Sbjct: 253 AVHDDANSNARPVQPTNKRIVHL 270

BLAST of CmoCh03G012020 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-62
Identity = 130/270 (48.15%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSISAQ--EVEDEREFDYREGSEKGPRHWGKIKKEWEACH 60
           MKI +   VFF L+  S+ + S S    EVEDE +F+Y +  EKGP +WG++K EW  C 
Sbjct: 1   MKIPSIGYVFF-LIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCG 60

Query: 61  NGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISI-YWVGKAG-SIKVNGV 120
            G +QSPIDL+ +RV I   LG L+  Y P NAT+KNRGHDI + +  G AG  I +NG 
Sbjct: 61  KGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGT 120

Query: 121 VYDLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQF 180
            Y LQQ HWHSPSEH++NG+R+ +E HM HQS D  G+N  AVV   Y  GKPD FL   
Sbjct: 121 EYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSKD--GRN--AVVAFFYKLGKPDYFLLTL 180

Query: 181 SKQISYMID-REHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVS 240
            + +  + D  E +  +  + P    F    YY+++GSLT PPCSE VIW I+K++RTV+
Sbjct: 181 ERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVT 240

Query: 241 KEQVRLLREAVHDYAERNARPLQPLNNREV 266
            +Q+ +LR  VHD +  NARPLQ  N R V
Sbjct: 241 LKQLIMLRVTVHDQSNSNARPLQRKNERPV 265

BLAST of CmoCh03G012020 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 236.5 bits (602), Expect = 2.5e-62
Identity = 117/267 (43.82%), Postives = 165/267 (61.80%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLLLFSLHLSSIS--AQEVEDEREFDYREGSEKGPRHWGKIKKEWEACH 60
           M      I F ++    L   +IS    EV+DE  F Y + +EKGP  WGKI   W+ C+
Sbjct: 1   MDTNAKTIFFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCN 60

Query: 61  NGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVVY 120
            G  QSPIDL+++RV ++       R YKP  A + NRGHDI + W G AG + +    +
Sbjct: 61  TGRYQSPIDLTNERVSLIHD-QAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDF 120

Query: 121 DLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSK 180
           +L Q HWHSPSEH++NG RYD+ELHM H S+    + + AV+G LY  G+P+ FL +   
Sbjct: 121 NLVQCHWHSPSEHTVNGTRYDLELHMVHTSA----RGRTAVIGVLYKLGEPNEFLTKLLN 180

Query: 181 QISYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKIRTVSKEQ 240
            I  + ++  E+ +G IDP +I+F   K+Y+Y+GSLTVPPC+EGVIW + K++ T+S EQ
Sbjct: 181 GIKAVGNK--EINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQ 240

Query: 241 VRLLREAVHDYAERNARPLQPLNNREV 266
           +  LR+AV D  E N+RP+Q    R V
Sbjct: 241 ITALRQAVDDGFETNSRPVQDSKGRSV 260

BLAST of CmoCh03G012020 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 222.6 bits (566), Expect = 3.8e-58
Identity = 106/208 (50.96%), Postives = 138/208 (66.35%), Query Frame = 0

Query: 28  VEDEREFDYREGSEKGPRHWGKIKKEWEACHNGVLQSPIDLSSQRVKIVPKLGVLKRSYK 87
           VEDE EF Y    E GP  WGK++ EW+ C  G +QSPIDL ++RV++V  L  L R YK
Sbjct: 5   VEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYK 64

Query: 88  PCNATVKNRGHDISI-YWVGKAGSIKVNGVVYDLQQAHWHSPSEHSINGRRYDMELHMFH 147
           PCNAT+KNRGHD+ + +    +GSI VNG  Y L Q HWHSPSEH++NGRR+ +ELHM H
Sbjct: 65  PCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVH 124

Query: 148 QSSDPNGKNKIAVVGQLYTTGKPDPFLAQFSKQISYMIDR-EHEMGIGEIDPADIKFGGN 207
           +    N    +AVV  LY  G+PD FL     ++S + D+ E E  +  IDP DIK G  
Sbjct: 125 E----NINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSR 184

Query: 208 KYYKYLGSLTVPPCSEGVIWVINKKIRT 234
           K+Y+Y+GSLT PPC++ VIW + KK+ T
Sbjct: 185 KFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of CmoCh03G012020 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 213.4 bits (542), Expect = 2.3e-55
Identity = 101/234 (43.16%), Postives = 149/234 (63.68%), Query Frame = 0

Query: 1   MKIGTTLIVFFSLL---LFSLHLSSISAQEVEDEREFDYREGSEKGPRHWGKIKKEWEAC 60
           M   T  I+FF +    LFS ++  + A+E+ ++  F Y++ +EKGP  WGK+  +W+ C
Sbjct: 1   MDANTKTILFFVVFFIDLFSPNILFVYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVC 60

Query: 61  HNGVLQSPIDLSSQRVKIVPKLGVLKRSYKPCNATVKNRGHDISIYWVGKAGSIKVNGVV 120
             G +QSPIDL+ +RV ++     L + YKP +A +++RGHD+ + W G  G I ++   
Sbjct: 61  STGKIQSPIDLTDERVSLIHD-QALSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTD 120

Query: 121 YDLQQAHWHSPSEHSINGRRYDMELHMFHQSSDPNGKNKIAVVGQLYTTGKPDPFLAQFS 180
           Y L Q HWHSPSEH+ING  YD+ELHM H S+      K  VVG LY  G+PD FL +  
Sbjct: 121 YKLVQCHWHSPSEHTINGTSYDLELHMVHTSA----SGKTTVVGVLYKLGEPDEFLTKIL 180

Query: 181 KQISYMIDREHEMGIGEIDPADIKFGGNKYYKYLGSLTVPPCSEGVIWVINKKI 232
             I  +  +E ++GI  +DP DI+F  N +Y+Y+GSLT+PPC+EGVIW + K++
Sbjct: 181 NGIKGVGKKEIDLGI--VDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTVQKRV 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8173.7e-7451.71Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
Q84UV83.5e-6446.39Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
Q9FM991.7e-6349.18Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
F4HUC41.6e-6148.15Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
F4JIK23.6e-6143.82Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EJ098.1e-157100.00Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111433054 PE=3 SV=1[more]
A0A6J1INE01.5e-13990.41Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111478003 PE=3 SV=1[more]
A0A0A0KKA62.7e-12078.11Carbonic anhydrase OS=Cucumis sativus OX=3659 GN=Csa_6G446560 PE=3 SV=1[more]
A0A6J1CS772.7e-12078.65Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111013713 PE=3 SV=1[more]
A0A1S3CIK81.0e-11975.91Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103500831 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022925725.11.7e-156100.00alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
KAG6604394.15.4e-15598.91Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_023544237.11.5e-14995.99alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022977815.13.2e-13990.41alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
XP_038881410.13.5e-12278.47alpha carbonic anhydrase 7-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G08080.12.6e-7551.71alpha carbonic anhydrase 7 [more]
AT1G08065.11.1e-6248.15alpha carbonic anhydrase 5 [more]
AT4G20990.12.5e-6243.82alpha carbonic anhydrase 4 [more]
AT2G28210.13.8e-5850.96alpha carbonic anhydrase 2 [more]
AT4G21000.12.3e-5543.16alpha carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 36..269
e-value: 5.8E-62
score: 221.8
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 35..266
e-value: 1.6E-42
score: 145.8
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 32..269
score: 45.211769
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 30..272
e-value: 5.0E-74
score: 250.8
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 33..265
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 7..271
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 7..271
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 129..145
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 43..266
e-value: 5.27249E-91
score: 266.83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G012020.1CmoCh03G012020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006730 one-carbon metabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding