CmoCh03G002540 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G002540
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionspermidine hydroxycinnamoyl transferase-like
LocationCmo_Chr03: 3861575 .. 3863249 (-)
RNA-Seq ExpressionCmoCh03G002540
SyntenyCmoCh03G002540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTTAACCGTTCATTCTTCCACCACCGTCTTCCCCGCCCACCCTACGCCCATTGCCACCCTCTCCTTCTCCGAGAGCGACCAAATCAAGCCATGGACTCACTCCCCCACCATCTATGTCTACCGATCCCCCGCTGTCCCCACCTCCGCCGTCGTCGACTCCTTGAAAAACTCCCTCTCCCAAATCCTCGTCCCTTACTACCCTCTCGTCGGCCGCCTCCACTGGATCGGTGGAGGCCGTCTTGAGCTTCATTGCTGCAGCGCCGGAGCTCAGCTCATTGAAGCCTCCTCTACCGCCACGCTCGACGATTATGGCGATTTCTCTCCCACTGATGCTCTTCGAGAGCTCGCTCCGAAAGTCGATTATAGTACTCCCATTGAGGAGCTGCCGGTGTTGTTGGTTCAGATGACGAGATTTAGCTGTGGTGGGATCGTGATCGGCATCGGCATTTCTCACATTCTTGTTGATGGGGTCTCGGCGATTACGTTTATCAACTCGTGGGCGAGTATCGCACGGAGGGAGAAGACGGTGGAGAGTATTGTGCAACCATTTCATGATCGGCGCATTTTGCAACCGCAGAAGCCGTTACGGCTGCCTAGGTTCGACCACCATGAATACAACAACCCGCCACTTCTTTTGGGTTGCTCGGATGCTAAGGTATGTGTATTAAATTCATAATTGTTGAATTTGACCCAGGTTAGAAATATGTCGAAACAAGTAAAAGAAGTAAAAGAAAAATTTACTGGGAGAGAGCTTTGGAACAAAGAAATCTTTAAGCAATTAGCTTATCAATTCTATTCTGGTATACGGCAAATAGTATACATATGAGTATTTACCGTGGAGAAAACACTTACTAGTTTCCTAAACTTTATTATTGAACCCTAACTTATTCCACCGATAAATTATTATTGATATTGAACTTTAGTGAACGGTTTAAGCTAATACATTCTAATATTCCCAACAGGAGGAGCGAAAGAAAGAATCCACCGTCACGTTGCTAAAACTTTCCAAAGAGCAAGTTGAAAAACTCAAAAAGAGAGCAAATTCTAACACAGTCACTCGACTCGACGAGGTACCATCACGACCATACACTCGGTACGAGTCTGTTGCAGGTCATATGTGGGTATGTGCATGCAAAGCTCGTAGCACCAACGACAACTCTCAACCGACGATGGTCCGTGTCGCTGTGGATGTACGACGACGACTTCGAACACCAATTCCCAACAACTTCACCGGAAACTCAACCCTAGTAGCCGTGACACCAAAGTGTCAATTCGACGATCTAATGTCTCAACCATTGAGCTACGCTGCTGGAAAGATTCGAGAAGGAACATGGAAGATCACGGACGAGTACGCGAGGTCGGCACTAGACTTCCTAGCAAGTCAAGAGGACATCAGCTGGGTTAGAACCTCGTACCACACAAAAGTTAAGGTTGAAGCTCCATTTTGGGGCAACCCCAATTTGTCTTTGGGAAGTTGGATGACGCTGCCGTTGTATGAGGCGGACTTCGGCTGGGGGCCGCCGTGTTATGTCGGGCCGGCGACATTGAATGCCGACGGGAAGTCGTTCATCATGCCGGCGCCGGACAACGACGGAGGGTTAGTTATAGCCGTACGGCTGCAGAAGAAGCACATGGATGATTTTAAGAGATTATTTTATGAAGATTGA

mRNA sequence

ATGGCTTCCTTAACCGTTCATTCTTCCACCACCGTCTTCCCCGCCCACCCTACGCCCATTGCCACCCTCTCCTTCTCCGAGAGCGACCAAATCAAGCCATGGACTCACTCCCCCACCATCTATGTCTACCGATCCCCCGCTGTCCCCACCTCCGCCGTCGTCGACTCCTTGAAAAACTCCCTCTCCCAAATCCTCGTCCCTTACTACCCTCTCGTCGGCCGCCTCCACTGGATCGGTGGAGGCCGTCTTGAGCTTCATTGCTGCAGCGCCGGAGCTCAGCTCATTGAAGCCTCCTCTACCGCCACGCTCGACGATTATGGCGATTTCTCTCCCACTGATGCTCTTCGAGAGCTCGCTCCGAAAGTCGATTATAGTACTCCCATTGAGGAGCTGCCGGTGTTGTTGGTTCAGATGACGAGATTTAGCTGTGGTGGGATCGTGATCGGCATCGGCATTTCTCACATTCTTGTTGATGGGGTCTCGGCGATTACGTTTATCAACTCGTGGGCGAGTATCGCACGGAGGGAGAAGACGGTGGAGAGTATTGTGCAACCATTTCATGATCGGCGCATTTTGCAACCGCAGAAGCCGTTACGGCTGCCTAGGTTCGACCACCATGAATACAACAACCCGCCACTTCTTTTGGGTTGCTCGGATGCTAAGGAGGAGCGAAAGAAAGAATCCACCGTCACGTTGCTAAAACTTTCCAAAGAGCAAGTTGAAAAACTCAAAAAGAGAGCAAATTCTAACACAGTCACTCGACTCGACGAGGTACCATCACGACCATACACTCGGTACGAGTCTGTTGCAGGTCATATGTGGGTATGTGCATGCAAAGCTCGTAGCACCAACGACAACTCTCAACCGACGATGGTCCGTGTCGCTGTGGATGTACGACGACGACTTCGAACACCAATTCCCAACAACTTCACCGGAAACTCAACCCTAGTAGCCGTGACACCAAAGTGTCAATTCGACGATCTAATGTCTCAACCATTGAGCTACGCTGCTGGAAAGATTCGAGAAGGAACATGGAAGATCACGGACGAGTACGCGAGGTCGGCACTAGACTTCCTAGCAAGTCAAGAGGACATCAGCTGGGTTAGAACCTCGTACCACACAAAAGTTAAGGTTGAAGCTCCATTTTGGGGCAACCCCAATTTGTCTTTGGGAAGTTGGATGACGCTGCCGTTGTATGAGGCGGACTTCGGCTGGGGGCCGCCGTGTTATGTCGGGCCGGCGACATTGAATGCCGACGGGAAGTCGTTCATCATGCCGGCGCCGGACAACGACGGAGGGTTAGTTATAGCCGTACGGCTGCAGAAGAAGCACATGGATGATTTTAAGAGATTATTTTATGAAGATTGA

Coding sequence (CDS)

ATGGCTTCCTTAACCGTTCATTCTTCCACCACCGTCTTCCCCGCCCACCCTACGCCCATTGCCACCCTCTCCTTCTCCGAGAGCGACCAAATCAAGCCATGGACTCACTCCCCCACCATCTATGTCTACCGATCCCCCGCTGTCCCCACCTCCGCCGTCGTCGACTCCTTGAAAAACTCCCTCTCCCAAATCCTCGTCCCTTACTACCCTCTCGTCGGCCGCCTCCACTGGATCGGTGGAGGCCGTCTTGAGCTTCATTGCTGCAGCGCCGGAGCTCAGCTCATTGAAGCCTCCTCTACCGCCACGCTCGACGATTATGGCGATTTCTCTCCCACTGATGCTCTTCGAGAGCTCGCTCCGAAAGTCGATTATAGTACTCCCATTGAGGAGCTGCCGGTGTTGTTGGTTCAGATGACGAGATTTAGCTGTGGTGGGATCGTGATCGGCATCGGCATTTCTCACATTCTTGTTGATGGGGTCTCGGCGATTACGTTTATCAACTCGTGGGCGAGTATCGCACGGAGGGAGAAGACGGTGGAGAGTATTGTGCAACCATTTCATGATCGGCGCATTTTGCAACCGCAGAAGCCGTTACGGCTGCCTAGGTTCGACCACCATGAATACAACAACCCGCCACTTCTTTTGGGTTGCTCGGATGCTAAGGAGGAGCGAAAGAAAGAATCCACCGTCACGTTGCTAAAACTTTCCAAAGAGCAAGTTGAAAAACTCAAAAAGAGAGCAAATTCTAACACAGTCACTCGACTCGACGAGGTACCATCACGACCATACACTCGGTACGAGTCTGTTGCAGGTCATATGTGGGTATGTGCATGCAAAGCTCGTAGCACCAACGACAACTCTCAACCGACGATGGTCCGTGTCGCTGTGGATGTACGACGACGACTTCGAACACCAATTCCCAACAACTTCACCGGAAACTCAACCCTAGTAGCCGTGACACCAAAGTGTCAATTCGACGATCTAATGTCTCAACCATTGAGCTACGCTGCTGGAAAGATTCGAGAAGGAACATGGAAGATCACGGACGAGTACGCGAGGTCGGCACTAGACTTCCTAGCAAGTCAAGAGGACATCAGCTGGGTTAGAACCTCGTACCACACAAAAGTTAAGGTTGAAGCTCCATTTTGGGGCAACCCCAATTTGTCTTTGGGAAGTTGGATGACGCTGCCGTTGTATGAGGCGGACTTCGGCTGGGGGCCGCCGTGTTATGTCGGGCCGGCGACATTGAATGCCGACGGGAAGTCGTTCATCATGCCGGCGCCGGACAACGACGGAGGGTTAGTTATAGCCGTACGGCTGCAGAAGAAGCACATGGATGATTTTAAGAGATTATTTTATGAAGATTGA

Protein sequence

MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED
Homology
BLAST of CmoCh03G002540 vs. ExPASy Swiss-Prot
Match: O64470 (Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT PE=1 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 5.3e-102
Identity = 194/460 (42.17%), Postives = 279/460 (60.65%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSA-VVDSLKN 60
           MA +T   S T+ PA PT       +E DQ+   TH PT+Y Y  P+      VV+ LK 
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120
           SLS++LV +YP+ GRL W+  GR EL+C + G + IEA S   L D+ DFSPT     L 
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTV 180
           P+V+Y  PIE +P+ L Q+T+F CGGI + + +SH +VDG SA+  I+ W  +AR E  +
Sbjct: 121 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-PL 180

Query: 181 ESIVQPFHDRRILQPQKPL----RLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKL 240
           E++  PF DR+IL   +PL      P+FDH E++ PP L+G +D  EERKK++ V +L L
Sbjct: 181 ETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 240

Query: 241 SKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVA 300
           S  Q++KL+ +AN +        P++ +TRYE+V GH+W CACKAR  +   QPT + + 
Sbjct: 241 STSQLQKLRSKANGSK----HSDPAKGFTRYETVTGHVWRCACKARG-HSPEQPTALGIC 300

Query: 301 VDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSA 360
           +D R R+  P+P  + GN+TL  V       +L+S  L +AA  I +    +T+EY    
Sbjct: 301 IDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAIKNVTNEYVMIG 360

Query: 361 LDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPAT 420
           +++L +Q+D+   +   H     E PF+GNPNL + SW+TLP+Y  DFGWG   Y GP T
Sbjct: 361 IEYLKNQKDLKKFQ-DLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYTGPGT 420

Query: 421 LNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
            + DG S I+P  + DG +++A  LQ  HM+ FK+ FYED
Sbjct: 421 HDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYED 450

BLAST of CmoCh03G002540 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.1e-83
Identity = 172/453 (37.97%), Postives = 260/453 (57.40%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQ 63
           + +  ST V PA  TPI  L  S  D + P  H+P++Y YR            +K +LS+
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSK 62

Query: 64  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVD 123
            LVP+YP+ GRL     GR+E+ C  AG   + A + + +DD+GDF+PT  LR+L P+VD
Sbjct: 63  ALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVD 122

Query: 124 YSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 183
           +S  I   P+L++Q+T F CGG  +G+G+ H   DG S + FIN+W+ +AR    ++  +
Sbjct: 123 HSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMAR---GLDLTI 182

Query: 184 QPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVE-- 243
            PF DR +L+ + P + P F H EY   P +    D  +   + +TV++ KL+++Q+   
Sbjct: 183 PPFIDRTLLRARDPPQ-PAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVAL 242

Query: 244 KLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRR 303
           K K + + NTV+         Y+ YE +AGH+W    KAR    N Q T + +A D R R
Sbjct: 243 KAKSKEDGNTVS---------YSSYEMLAGHVWRSVGKARGL-PNDQETKLYIATDGRSR 302

Query: 304 LRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLAS 363
           LR  +P  + GN  +   TP     DL+S+P  YAAG+I +   ++ D Y RSALD+L  
Sbjct: 303 LRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYLEM 362

Query: 364 QEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADGK 423
           Q D+S +    HT        +  PNL + SW+ LP+Y+ADFGWG P ++GP  +  +G 
Sbjct: 363 QPDLSALVRGAHT--------YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPYEGL 422

Query: 424 SFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           SF++P+P NDG L +A+ LQ +HM  F++  +E
Sbjct: 423 SFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of CmoCh03G002540 vs. ExPASy Swiss-Prot
Match: Q8GSM7 (Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.3e-81
Identity = 171/455 (37.58%), Postives = 257/455 (56.48%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQ 63
           + V  ST V PA  TP   L  S  D + P  H+P++Y YR    P       LK +LS+
Sbjct: 3   IEVKESTMVKPAAETPQQRLWNSNVDLVVPNFHTPSVYFYRPTGSPNFFDGKVLKEALSK 62

Query: 64  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVD 123
            LVP+YP+ GRL     GR+E+ C   G   +EA S   +DD+GDF+PT  LR+L P VD
Sbjct: 63  ALVPFYPMAGRLCRDEDGRIEIDCKGQGVLFVEAESDGVVDDFGDFAPTLELRQLIPAVD 122

Query: 124 YSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 183
           YS  I+   +L++Q+T F CGG+ +G+G+ H   DG S + FIN+W+ +AR    ++  +
Sbjct: 123 YSQGIQSYALLVLQITHFKCGGVSLGVGMQHHAADGASGLHFINTWSDMAR---GLDLTI 182

Query: 184 QPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAK--EERKKESTVTLLKLSKEQVE 243
            PF DR +L+ + P + P+F H EY  PP L    +     E   E++V++ KL+++Q+ 
Sbjct: 183 PPFIDRTLLRARDPPQ-PQFPHVEYQPPPTLKVTPENTPISEAVPETSVSIFKLTRDQIN 242

Query: 244 --KLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVR 303
             K K + + NTV          Y+ YE +AGH+W   C AR    + Q T + +A D R
Sbjct: 243 TLKAKSKEDGNTVN---------YSSYEMLAGHVWRSTCMARGL-AHDQETKLYIATDGR 302

Query: 304 RRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFL 363
            RLR  +P  + GN  +   TP     D+ S+P+ YAA K+ +   ++ ++Y RSALD+L
Sbjct: 303 SRLRPSLPPGYFGN-VIFTTTPIAVAGDIQSKPIWYAASKLHDALARMDNDYLRSALDYL 362

Query: 364 ASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNAD 423
             Q D+  +    HT        +  PNL + SW  LP+++ADFGWG P ++GP  +  +
Sbjct: 363 ELQPDLKALVRGAHT--------FKCPNLGITSWSRLPIHDADFGWGRPIFMGPGGIAYE 422

Query: 424 GKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           G SFI+P+P NDG   +A+ LQ +HM  F++  Y+
Sbjct: 423 GLSFILPSPTNDGSQSVAISLQAEHMKLFEKFLYD 434

BLAST of CmoCh03G002540 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.3e-76
Identity = 169/461 (36.66%), Postives = 256/461 (55.53%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDS--LKNSL 63
           + +  +T V P+ PTP   L  S  D + P  H+P++Y YR P    S   D+  LK +L
Sbjct: 3   INIKETTMVRPSQPTPSQRLWNSNLDLVVPRFHTPSVYFYRRPG-NASDFFDARVLKEAL 62

Query: 64  SQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPK 123
            + LVP+YP+ GRL     GR+E+ C   G + + A + + +D++GDF+PT  L++L PK
Sbjct: 63  GRALVPFYPMAGRLARDEDGRVEIDCNGEGVRFVVAETDSAIDEFGDFAPTMELKKLIPK 122

Query: 124 VDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVES 183
           V+Y   I   P+L++Q+T F CGG  +G+G+ H + DG S + FINSW+ IAR    ++ 
Sbjct: 123 VEYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIAR---GLDI 182

Query: 184 IVQPFHDRRILQPQKPLRLPRFDHHEY------NNPPLLLGCSDAKEERKKESTVTLLKL 243
            V PF DR +L+ + P   P F H EY      N  P  +     K +    + V++ KL
Sbjct: 183 AVPPFIDRSLLRARDP-PSPSFPHIEYQPAPSMNTSPAPIQDPTVKSD-PTATAVSIFKL 242

Query: 244 SKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVA 303
           +K+Q++ LK R             S  Y+ Y  VAGH+W C   AR   D+ Q T +  A
Sbjct: 243 TKQQLDLLKSRV------------SAKYSSYALVAGHVWRCTSIARGLPDD-QRTKLYCA 302

Query: 304 VDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQP--LSYAAGKIREGTWKITDEYAR 363
            D R RL+ P+P+ + GN  +   TP     ++  +   L  AAG+I+    ++ DEY R
Sbjct: 303 TDGRARLQPPLPSGYFGN-VIFTATPVADAGEITGEDSGLEAAAGRIQRALMRMDDEYLR 362

Query: 364 SALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGP 423
           SALD+L  Q D+S +    HT        +  PN+ L SW  LP+++ADFGWG P ++GP
Sbjct: 363 SALDYLELQPDLSKLVRGAHT--------FRCPNIGLTSWTRLPIHDADFGWGRPIFMGP 422

Query: 424 ATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
             +  +G +F++P+ + DG L IA+ LQ +HM  F++L YE
Sbjct: 423 GGIAYEGLAFMLPSSEGDGSLSIAISLQAEHMIKFQKLLYE 435

BLAST of CmoCh03G002540 vs. ExPASy Swiss-Prot
Match: Q0JBZ8 (Hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT1 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 3.7e-71
Identity = 160/462 (34.63%), Postives = 253/462 (54.76%), Query Frame = 0

Query: 3   SLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVV-----DSL 62
           ++TV  ST V PA  TP   L  S  D + P  H+P++Y YR       A       + +
Sbjct: 2   AITVRRSTMVRPAWETPRVRLWNSNLDLVVPRFHTPSVYFYRRGPEGGGAPEGFFDGERM 61

Query: 63  KNSLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASS-TATLDDYGDFSPTDALR 122
           + +L++ LVP+YP+ GRL     GR+E+ C   G   +EA +  A++DDYGDF+PT  L+
Sbjct: 62  RRALAEALVPFYPMAGRLARDEDGRVEIDCNGEGVLFVEADAPDASVDDYGDFAPTMELK 121

Query: 123 ELAPKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARRE 182
            L P VDY+  I    +L++Q+T F CGG+ +G+G+ H + DG+S + FINSW+ + R  
Sbjct: 122 RLIPAVDYTDDISSFSLLVLQVTYFKCGGVSLGVGMQHHVADGMSGLHFINSWSDLCR-- 181

Query: 183 KTVESIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLG----CSDAKEERKKESTVTL 242
              +  + PF DR +L+ + P   P + H EY   P +L        A +     + V +
Sbjct: 182 -GTQIAIMPFIDRTLLRARDP-PTPSYPHVEYQPAPAMLSSVPQSVTANKTTPPPTAVDI 241

Query: 243 LKLSKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMV 302
            KL++  + +L+ +  S      +  P   ++ Y  +A H+W C   AR    + QPT +
Sbjct: 242 FKLTRSDLGRLRSQLPSG-----EGAPR--FSTYAVLAAHVWRCVSLARGL-PSEQPTKL 301

Query: 303 RVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYA 362
             A D R+RL+ P+P  + GN  +   TP  +   + S  L+  A  I+E   ++ D Y 
Sbjct: 302 YCATDGRQRLQPPLPEGYFGN-VIFTATPLAEAGKVTS-GLADGAAVIQEALDRMNDSYC 361

Query: 363 RSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVG 422
           RSALD+L  Q D+S +    HT        +  PNL L SW+ LP+++ADFGWG P ++G
Sbjct: 362 RSALDYLELQPDLSALVRGAHT--------FRCPNLGLTSWVRLPIHDADFGWGRPVFMG 421

Query: 423 PATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           P  +  +G +F++P+ + DG L IA+ LQ +HM+ F++L +E
Sbjct: 422 PGGIAYEGLAFVLPSANKDGSLSIAISLQAEHMEKFRKLIFE 441

BLAST of CmoCh03G002540 vs. ExPASy TrEMBL
Match: A0A6J1GF56 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453622 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 2.7e-266
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS
Sbjct: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE
Sbjct: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV
Sbjct: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED
Sbjct: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 455

BLAST of CmoCh03G002540 vs. ExPASy TrEMBL
Match: A0A6J1IM00 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita maxima OX=3661 GN=LOC111478230 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.6e-248
Identity = 418/455 (91.87%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLT+HSST VFPAHPTP  TLSFSESDQIKPWTHSPT+YV+RSPAVPTSAVVDSLKNS
Sbjct: 1   MASLTIHSSTAVFPAHPTPTTTLSFSESDQIKPWTHSPTVYVFRSPAVPTSAVVDSLKNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQ+LVPYYPL GRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLL+Q+TRFSCGGIVIG GISHILVDGVSAITFINSWASIAR+EKTVE
Sbjct: 121 KVDYSTPIEELPVLLIQVTRFSCGGIVIGTGISHILVDGVSAITFINSWASIARKEKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDR +LQPQKPLR PRFDH EY+NPP LLGC DAKEERKKES  TLLKLSKEQV
Sbjct: 181 SIVQPFHDRSVLQPQKPLRSPRFDHREYDNPPFLLGCLDAKEERKKESIPTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRAN N V RLDE+PSRPYTRYE++AGHMWVCACKARST +NSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANLNLVNRLDELPSRPYTRYETIAGHMWVCACKARSTGNNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RL TPIPNNFTGNSTLVAVTP CQFDDL+SQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLGTPIPNNFTGNSTLVAVTPMCQFDDLISQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWGPPCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAP NDGGL+IA+RLQKKHMDDFKR FYED
Sbjct: 421 KSFIMPAPGNDGGLIIAIRLQKKHMDDFKRHFYED 455

BLAST of CmoCh03G002540 vs. ExPASy TrEMBL
Match: A0A6J1GCX6 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453028 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.8e-247
Identity = 416/455 (91.43%), Postives = 436/455 (95.82%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLT+HSSTTVFPAHPTPIATLSFSESDQIKPWTHSPT+YVYRSP V TSAVV+SL+NS
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPTVTTSAVVESLRNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQ+LVPYYPL GRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPV L+Q+TRFSCGGIVIG G+SHILVDGVSAITFINSWASIAR++KTVE
Sbjct: 121 KVDYSTPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARKDKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDR +LQPQKPLR PRFDH EY+NPP LLGCSDAKEERKKES  TLLKLSKEQV
Sbjct: 181 SIVQPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRANSN V +LDE+PSRPYTRYES+AGHMWVCACKARST D SQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANSNIVNQLDELPSRPYTRYESIAGHMWVCACKARSTGDKSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RL TP+PNNFTGNSTLVAVTPKCQF DL+SQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWG PCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAPDNDGGL+IA+RLQKKHMDDFKR FYED
Sbjct: 421 KSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYED 455

BLAST of CmoCh03G002540 vs. ExPASy TrEMBL
Match: A0A6J1DRJ9 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023179 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 3.0e-188
Identity = 320/467 (68.52%), Postives = 388/467 (83.08%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSP-AVPTSAVVDSLKN 60
           MASLT++SSTTVFP+HPTP A LS SE+DQ + WTH PT+Y+Y+SP A  T+AV+ SLKN
Sbjct: 1   MASLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSPAAAATAAVLGSLKN 60

Query: 61  SLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120
           SLSQILVPYYPL GRL  I GGRLEL CC+AGA L+EASSTA LDDYGDF+P+DALR LA
Sbjct: 61  SLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDALRRLA 120

Query: 121 PKVDY-STPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKT 180
           P VDY ++PIEE+P+LLVQ+TRFSCGG+V+G+  SH LVDG SAITFINSWAS+AR EKT
Sbjct: 121 PNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMARGEKT 180

Query: 181 VESIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKE 240
           V+SI QPFHDR+ LQPQKP+  PRF H EY+ PPLL G S+A+EE KKES   LLKL+KE
Sbjct: 181 VDSIPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALLKLTKE 240

Query: 241 QVEKLKKRANSN---------TVTRLD-EVPSRPYTRYESVAGHMWVCACKARSTNDNSQ 300
           QVEKLKKRANSN          +  LD E+  RPY+RYES+ G+MW+CACKAR+  ++ Q
Sbjct: 241 QVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNNAESRQ 300

Query: 301 PTMVRVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKIT 360
           PT+VR+ VD+ RRLRTPIP NFTGNS L+ +TP+C+F DLM+QPLSYA GKIREGTWK+T
Sbjct: 301 PTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREGTWKMT 360

Query: 361 DEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPP 420
           DEYA+SA+DFLA +EDI+W RT +H+ V+V+  FWGNPNL+LGSWM++PLY+ADFGWG P
Sbjct: 361 DEYAKSAMDFLARKEDITWARTLHHSIVRVKGSFWGNPNLALGSWMSMPLYDADFGWGRP 420

Query: 421 CYVGPATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
            YVGPA +N+DGK++IM  P NDG ++IA+RLQ KHMDDFK+ FYED
Sbjct: 421 SYVGPAMVNSDGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYED 467

BLAST of CmoCh03G002540 vs. ExPASy TrEMBL
Match: A0A6J1HTD7 (spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466005 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 5.8e-184
Identity = 303/460 (65.87%), Postives = 381/460 (82.83%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MAS+T+HSS+TV P+HPTP  T SFSE DQ + WTH PTIYVY+SPA    A V+SLK S
Sbjct: 1   MASITIHSSSTVVPSHPTPTGTFSFSECDQYRAWTHLPTIYVYKSPATEVVA-VESLKTS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQILVPYYPL GRLHW+ GGRLEL CC+AG   +EAS+ A L+DYGDF+P+DA+R+L P
Sbjct: 61  LSQILVPYYPLAGRLHWLPGGRLELDCCAAGVLFVEASTDAKLEDYGDFAPSDAVRQLIP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
            VDY++PIEELP+LL Q+TRFSCGG+VIG+   H +VDG SAITFINSWAS+AR EKTVE
Sbjct: 121 TVDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARGEKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNN-PPLLLGCSDAKEERKKESTVTLLKLSKEQ 240
           +I+QP HDR+ L P+KPLR PRFDH E+   PP++LG S+ +EE K+E+T  LLKL+KEQ
Sbjct: 181 TILQPLHDRKFLMPEKPLRPPRFDHIEFTKPPPVILGSSNPEEEAKQETTSALLKLTKEQ 240

Query: 241 VEKLKKRANSN----TVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVA 300
           +EKLKK+ANSN    T+   DEV  RPY+R+E++ GHMWVCACKAR+T ++ QPT++R+ 
Sbjct: 241 IEKLKKKANSNLTNQTMGVADEVQPRPYSRFEAITGHMWVCACKARNTGESRQPTVIRIP 300

Query: 301 VDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSA 360
           +DVRRR++ PIP NF+GNS+ V +TP+C+F +LMSQPLSYAAGK+REGTWK+T EYA+S 
Sbjct: 301 IDVRRRMQPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTGEYAQSV 360

Query: 361 LDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPAT 420
           +D+LA Q+DISW+RTS+H+KV V+A FWGNPNLS+GSW++LP YEADFGWG P Y GPAT
Sbjct: 361 VDYLAGQDDISWLRTSFHSKVLVKASFWGNPNLSIGSWISLPFYEADFGWGRPSYFGPAT 420

Query: 421 LNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           LN DGKS+IM  P +DG L++A+RLQ +HMD FK+ FYED
Sbjct: 421 LNIDGKSYIMVGPSDDGSLIVAIRLQARHMDGFKKYFYED 459

BLAST of CmoCh03G002540 vs. NCBI nr
Match: XP_022950556.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata])

HSP 1 Score: 927.2 bits (2395), Expect = 5.6e-266
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS
Sbjct: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE
Sbjct: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV
Sbjct: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED
Sbjct: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 455

BLAST of CmoCh03G002540 vs. NCBI nr
Match: KAG6603488.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 442/452 (97.79%), Postives = 450/452 (99.56%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQ 63
           LT+HSSTTVFPAHPTPIATLSFSESDQIKPWTHSPT+YVYRSPAVPTSAVVDSL+NSLSQ
Sbjct: 13  LTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAVPTSAVVDSLRNSLSQ 72

Query: 64  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVD 123
           ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALR+LAPKVD
Sbjct: 73  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRKLAPKVD 132

Query: 124 YSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 183
           YSTPIEELPVLLVQ+TRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV
Sbjct: 133 YSTPIEELPVLLVQITRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 192

Query: 184 QPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVEKL 243
           +PFHDR +LQPQKPLR PRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVEKL
Sbjct: 193 KPFHDRSVLQPQKPLRPPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVEKL 252

Query: 244 KKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRRLR 303
           KKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRRLR
Sbjct: 253 KKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRRLR 312

Query: 304 TPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLASQE 363
           TPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLASQE
Sbjct: 313 TPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLASQE 372

Query: 364 DISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADGKSF 423
           DISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWGPPCYVGPATLNADGKSF
Sbjct: 373 DISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGPATLNADGKSF 432

Query: 424 IMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           IMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED
Sbjct: 433 IMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 464

BLAST of CmoCh03G002540 vs. NCBI nr
Match: XP_023544822.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 434/455 (95.38%), Postives = 447/455 (98.24%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLT+HSSTTVFPAHPTPIATLSFSESDQIKPWTHSPT+YVYRSPAV TS VV+SL+NS
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAVATSTVVESLRNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQ+LVPYYPL GRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLLVQ+TRFSCGGIVIGIGISHILVDGVSAITFINSWASIAR+EKTVE
Sbjct: 121 KVDYSTPIEELPVLLVQITRFSCGGIVIGIGISHILVDGVSAITFINSWASIARKEKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDR ILQPQKPLR PRFDH EY+NPP LLGC DAKEERKKESTVTLLKLSKEQV
Sbjct: 181 SIVQPFHDRSILQPQKPLRPPRFDHGEYDNPPFLLGCLDAKEERKKESTVTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRANSNTVT+LDEVPSRPYTRYESVAGHMWVCACKAR+TNDNSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANSNTVTQLDEVPSRPYTRYESVAGHMWVCACKARNTNDNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RLRTPIPNNFTGNSTLVAVTPKCQF+DL+SQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLRTPIPNNFTGNSTLVAVTPKCQFNDLISQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWGPPCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED
Sbjct: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 455

BLAST of CmoCh03G002540 vs. NCBI nr
Match: KAG6603487.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 874.8 bits (2259), Expect = 3.3e-250
Identity = 424/455 (93.19%), Postives = 441/455 (96.92%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLT+HSSTTVFPAHPTPIATLSFSESDQIKPWTHSPT+YVYRSPAV TSAVV+SL+NS
Sbjct: 1   MASLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSPAVTTSAVVESLRNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQ+LVPYYPL GRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIAR+EKTVE
Sbjct: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARKEKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIV PFHDR +LQPQKPLR PRFDH EY+NPP LLGCSDAKEERKKES  TLLKLSKEQV
Sbjct: 181 SIVLPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRANSN VT+LDE+PSRPYTRYES+AGHMWVCACKARST+DNSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANSNIVTQLDELPSRPYTRYESIAGHMWVCACKARSTSDNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RL TP+PNNFTGNSTLVAVTPKCQF DL+SQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWG PCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAPDNDGGL+IA+RLQKKHMDDFKR FYED
Sbjct: 421 KSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYED 455

BLAST of CmoCh03G002540 vs. NCBI nr
Match: XP_022978166.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita maxima])

HSP 1 Score: 865.5 bits (2235), Expect = 2.0e-247
Identity = 418/455 (91.87%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNS 60
           MASLT+HSST VFPAHPTP  TLSFSESDQIKPWTHSPT+YV+RSPAVPTSAVVDSLKNS
Sbjct: 1   MASLTIHSSTAVFPAHPTPTTTLSFSESDQIKPWTHSPTVYVFRSPAVPTSAVVDSLKNS 60

Query: 61  LSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120
           LSQ+LVPYYPL GRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP
Sbjct: 61  LSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAP 120

Query: 121 KVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVE 180
           KVDYSTPIEELPVLL+Q+TRFSCGGIVIG GISHILVDGVSAITFINSWASIAR+EKTVE
Sbjct: 121 KVDYSTPIEELPVLLIQVTRFSCGGIVIGTGISHILVDGVSAITFINSWASIARKEKTVE 180

Query: 181 SIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQV 240
           SIVQPFHDR +LQPQKPLR PRFDH EY+NPP LLGC DAKEERKKES  TLLKLSKEQV
Sbjct: 181 SIVQPFHDRSVLQPQKPLRSPRFDHREYDNPPFLLGCLDAKEERKKESIPTLLKLSKEQV 240

Query: 241 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 300
           EKLKKRAN N V RLDE+PSRPYTRYE++AGHMWVCACKARST +NSQPTMVRVAVDVRR
Sbjct: 241 EKLKKRANLNLVNRLDELPSRPYTRYETIAGHMWVCACKARSTGNNSQPTMVRVAVDVRR 300

Query: 301 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 360
           RL TPIPNNFTGNSTLVAVTP CQFDDL+SQPLSYAAGKIREGTWKITDEYARSALDFLA
Sbjct: 301 RLGTPIPNNFTGNSTLVAVTPMCQFDDLISQPLSYAAGKIREGTWKITDEYARSALDFLA 360

Query: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 420
           SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWM+LPLYEADFGWGPPCYVGPATLNADG
Sbjct: 361 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMSLPLYEADFGWGPPCYVGPATLNADG 420

Query: 421 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
           KSFIMPAP NDGGL+IA+RLQKKHMDDFKR FYED
Sbjct: 421 KSFIMPAPGNDGGLIIAIRLQKKHMDDFKRHFYED 455

BLAST of CmoCh03G002540 vs. TAIR 10
Match: AT2G19070.1 (spermidine hydroxycinnamoyl transferase )

HSP 1 Score: 372.9 bits (956), Expect = 3.8e-103
Identity = 194/460 (42.17%), Postives = 279/460 (60.65%), Query Frame = 0

Query: 1   MASLTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSA-VVDSLKN 60
           MA +T   S T+ PA PT       +E DQ+   TH PT+Y Y  P+      VV+ LK 
Sbjct: 1   MAPITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGNVVEILKT 60

Query: 61  SLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELA 120
           SLS++LV +YP+ GRL W+  GR EL+C + G + IEA S   L D+ DFSPT     L 
Sbjct: 61  SLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFSPTPEFENLM 120

Query: 121 PKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTV 180
           P+V+Y  PIE +P+ L Q+T+F CGGI + + +SH +VDG SA+  I+ W  +AR E  +
Sbjct: 121 PQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE-PL 180

Query: 181 ESIVQPFHDRRILQPQKPL----RLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKL 240
           E++  PF DR+IL   +PL      P+FDH E++ PP L+G +D  EERKK++ V +L L
Sbjct: 181 ETV--PFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVVMLPL 240

Query: 241 SKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVA 300
           S  Q++KL+ +AN +        P++ +TRYE+V GH+W CACKAR  +   QPT + + 
Sbjct: 241 STSQLQKLRSKANGSK----HSDPAKGFTRYETVTGHVWRCACKARG-HSPEQPTALGIC 300

Query: 301 VDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSA 360
           +D R R+  P+P  + GN+TL  V       +L+S  L +AA  I +    +T+EY    
Sbjct: 301 IDTRSRMEPPLPRGYFGNATLDVVAASTS-GELISNELGFAASLISKAIKNVTNEYVMIG 360

Query: 361 LDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPAT 420
           +++L +Q+D+   +   H     E PF+GNPNL + SW+TLP+Y  DFGWG   Y GP T
Sbjct: 361 IEYLKNQKDLKKFQ-DLHALGSTEGPFYGNPNLGVVSWLTLPMYGLDFGWGKEFYTGPGT 420

Query: 421 LNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYED 456
            + DG S I+P  + DG +++A  LQ  HM+ FK+ FYED
Sbjct: 421 HDFDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYED 450

BLAST of CmoCh03G002540 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 312.0 bits (798), Expect = 7.9e-85
Identity = 172/453 (37.97%), Postives = 260/453 (57.40%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQ 63
           + +  ST V PA  TPI  L  S  D + P  H+P++Y YR            +K +LS+
Sbjct: 3   INIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSK 62

Query: 64  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVD 123
            LVP+YP+ GRL     GR+E+ C  AG   + A + + +DD+GDF+PT  LR+L P+VD
Sbjct: 63  ALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDFAPTLNLRQLIPEVD 122

Query: 124 YSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 183
           +S  I   P+L++Q+T F CGG  +G+G+ H   DG S + FIN+W+ +AR    ++  +
Sbjct: 123 HSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMAR---GLDLTI 182

Query: 184 QPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLGCSDAKEERKKESTVTLLKLSKEQVE-- 243
            PF DR +L+ + P + P F H EY   P +    D  +   + +TV++ KL+++Q+   
Sbjct: 183 PPFIDRTLLRARDPPQ-PAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVAL 242

Query: 244 KLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRRR 303
           K K + + NTV+         Y+ YE +AGH+W    KAR    N Q T + +A D R R
Sbjct: 243 KAKSKEDGNTVS---------YSSYEMLAGHVWRSVGKARGL-PNDQETKLYIATDGRSR 302

Query: 304 LRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLAS 363
           LR  +P  + GN  +   TP     DL+S+P  YAAG+I +   ++ D Y RSALD+L  
Sbjct: 303 LRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRSALDYLEM 362

Query: 364 QEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADGK 423
           Q D+S +    HT        +  PNL + SW+ LP+Y+ADFGWG P ++GP  +  +G 
Sbjct: 363 QPDLSALVRGAHT--------YKCPNLGITSWVRLPIYDADFGWGRPIFMGPGGIPYEGL 422

Query: 424 SFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           SF++P+P NDG L +A+ LQ +HM  F++  +E
Sbjct: 423 SFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of CmoCh03G002540 vs. TAIR 10
Match: AT5G57840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-51
Identity = 138/454 (30.40%), Postives = 211/454 (46.48%), Query Frame = 0

Query: 4   LTVHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTIYVYRSPAVPTSAVVDSLKNSLSQ 63
           + V  +T V PA  TP   L  S  D I+   H  T+Y Y+  +        SL  +LS+
Sbjct: 3   IRVKQATIVKPAEETPTHNLWLSNLDLIQVRLHMGTLYFYKPCSSSDRPNTQSLIEALSK 62

Query: 64  ILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDALRELAPKVD 123
           +LV +YP  GRL     GRLE+ C   G   +EA S +T+ D G  + +  L +L P VD
Sbjct: 63  VLVFFYPAAGRLQKNTNGRLEVQCNGEGVLFVEAESDSTVQDIGLLTQSLDLSQLVPTVD 122

Query: 124 YSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARREKTVESIV 183
           Y+  I   P+LL Q+T F CG I +G  I H   +  S    + +W S+  R   V+  +
Sbjct: 123 YAGDISSYPLLLFQVTYFKCGTICVGSSIHHTFGEATSLGYIMEAW-SLTARGLLVK--L 182

Query: 184 QPFHDRRILQPQKPLRLPRFDHHEYNNPPL---LLGCSDAKEERKKESTVTLLKLSKEQV 243
            PF DR +L  + P     F H EY +PP     +     +   + +S +  LKL++ Q+
Sbjct: 183 TPFLDRTVLHARNP-PSSVFPHTEYQSPPFHNHPMKSLAYRSNPESDSAIATLKLTRLQL 242

Query: 244 EKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWVCACKARSTNDNSQPTMVRVAVDVRR 303
           + LK RA         E+    ++ YE +  H W CA  A         T + + +D R 
Sbjct: 243 KALKARA---------EIADSKFSTYEVLVAHTWRCASFANEDLSEEHSTRLHIIIDGRP 302

Query: 304 RLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSYAAGKIREGTWKITDEYARSALDFLA 363
           RL+  +P  + GN TL    P         +  S    ++     K+ +EY RSA+D+L 
Sbjct: 303 RLQPKLPQGYIGN-TLFHARPVSPLGAFHRESFSETVERVHREIRKMDNEYLRSAIDYLE 362

Query: 364 SQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMTLPLYEADFGWGPPCYVGPATLNADG 423
              D+  +       +     F    N  + S +    YE DFGWG   Y   + LN +G
Sbjct: 363 RHPDLDQLVPGEGNTI-----FSCAANFCIVSLIKQTAYEMDFGWGKAYYKRASHLN-EG 422

Query: 424 KSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           K F+   PD +G +++ + L+K  +  F++LFYE
Sbjct: 423 KGFVTGNPDEEGSMMLTMCLKKTQLSKFRKLFYE 436

BLAST of CmoCh03G002540 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 121/419 (28.88%), Postives = 198/419 (47.26%), Query Frame = 0

Query: 39  TIYVYRSPAVPTSAVVDSLKNSLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEAS 98
           TIY ++S        V  +K +LSQ+LV YYPL GRL     G+L + C   G   +EA 
Sbjct: 67  TIYCFKSEERGNEEAVQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAE 126

Query: 99  STATLDDYGDFSPTD--ALRELAPKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHIL 158
           +   +D+ GD +  D   L +L   V  +  I E+P +  Q+T+F CGG V+G+ ++H +
Sbjct: 127 ANCKMDEIGDITKPDPETLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCM 186

Query: 159 VDGVSAITFINSWASIARREKTVESIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLG 218
            DG+ A+ F+NSW  +AR    +     PF DR IL  + P ++    H E+     +  
Sbjct: 187 FDGIGAMEFVNSWGQVAR---GLPLTTPPFSDRTILNARNPPKIENL-HQEFEE---IED 246

Query: 219 CSDAKEERKKESTV-TLLKLSKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWV 278
            S+      KE T+        E+++KLK +A  N+ + L        T +E+++  +W 
Sbjct: 247 KSNINSLYTKEPTLYRSFCFDPEKIKKLKLQATENSESLL----GNSCTSFEALSAFVWR 306

Query: 279 CACKARSTNDNSQPTMVRVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSY 338
              K+     + Q T +  AVD R +    +P  + GN  +V     C+  +L+ +PLS+
Sbjct: 307 ARTKSLKML-SDQKTKLLFAVDGRAKFEPQLPKGYFGNG-IVLTNSICEAGELIEKPLSF 366

Query: 339 AAGKIREGTWKITDEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMT 398
           A G +RE    +TD Y RSA+D+     +++  R S             +  L + +W  
Sbjct: 367 AVGLVREAIKMVTDGYMRSAIDYF----EVTRARPSL------------SSTLLITTWSR 426

Query: 399 LPLYEADFGWGPPCYVGPATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           L  +  DFGWG P   GP  L     +  +   +    + + + L    MD F+  F +
Sbjct: 427 LGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 456

BLAST of CmoCh03G002540 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-44
Identity = 121/419 (28.88%), Postives = 198/419 (47.26%), Query Frame = 0

Query: 39  TIYVYRSPAVPTSAVVDSLKNSLSQILVPYYPLVGRLHWIGGGRLELHCCSAGAQLIEAS 98
           TIY ++S        V  +K +LSQ+LV YYPL GRL     G+L + C   G   +EA 
Sbjct: 51  TIYCFKSEERGNEEAVQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAE 110

Query: 99  STATLDDYGDFSPTD--ALRELAPKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHIL 158
           +   +D+ GD +  D   L +L   V  +  I E+P +  Q+T+F CGG V+G+ ++H +
Sbjct: 111 ANCKMDEIGDITKPDPETLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCM 170

Query: 159 VDGVSAITFINSWASIARREKTVESIVQPFHDRRILQPQKPLRLPRFDHHEYNNPPLLLG 218
            DG+ A+ F+NSW  +AR    +     PF DR IL  + P ++    H E+     +  
Sbjct: 171 FDGIGAMEFVNSWGQVAR---GLPLTTPPFSDRTILNARNPPKIENL-HQEFEE---IED 230

Query: 219 CSDAKEERKKESTV-TLLKLSKEQVEKLKKRANSNTVTRLDEVPSRPYTRYESVAGHMWV 278
            S+      KE T+        E+++KLK +A  N+ + L        T +E+++  +W 
Sbjct: 231 KSNINSLYTKEPTLYRSFCFDPEKIKKLKLQATENSESLL----GNSCTSFEALSAFVWR 290

Query: 279 CACKARSTNDNSQPTMVRVAVDVRRRLRTPIPNNFTGNSTLVAVTPKCQFDDLMSQPLSY 338
              K+     + Q T +  AVD R +    +P  + GN  +V     C+  +L+ +PLS+
Sbjct: 291 ARTKSLKML-SDQKTKLLFAVDGRAKFEPQLPKGYFGNG-IVLTNSICEAGELIEKPLSF 350

Query: 339 AAGKIREGTWKITDEYARSALDFLASQEDISWVRTSYHTKVKVEAPFWGNPNLSLGSWMT 398
           A G +RE    +TD Y RSA+D+     +++  R S             +  L + +W  
Sbjct: 351 AVGLVREAIKMVTDGYMRSAIDYF----EVTRARPSL------------SSTLLITTWSR 410

Query: 399 LPLYEADFGWGPPCYVGPATLNADGKSFIMPAPDNDGGLVIAVRLQKKHMDDFKRLFYE 455
           L  +  DFGWG P   GP  L     +  +   +    + + + L    MD F+  F +
Sbjct: 411 LGFHTTDFGWGEPILSGPVALPEKEVTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 440

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644705.3e-10242.17Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT P... [more]
Q9FI781.1e-8337.97Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST P... [more]
Q8GSM72.3e-8137.58Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1... [more]
A0A2H5AIZ11.3e-7636.66Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV... [more]
Q0JBZ83.7e-7134.63Hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT1 P... [more]
Match NameE-valueIdentityDescription
A0A6J1GF562.7e-266100.00spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IM009.6e-24891.87spermidine hydroxycinnamoyl transferase-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GCX62.8e-24791.43spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DRJ93.0e-18868.52spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1HTD75.8e-18465.87spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
XP_022950556.15.6e-266100.00spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata][more]
KAG6603488.12.1e-26097.79Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023544822.13.4e-25595.38spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo][more]
KAG6603487.13.3e-25093.19Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022978166.12.0e-24791.87spermidine hydroxycinnamoyl transferase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G19070.13.8e-10342.17spermidine hydroxycinnamoyl transferase [more]
AT5G48930.17.9e-8537.97hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase [more]
AT5G57840.11.2e-5130.40HXXXD-type acyl-transferase family protein [more]
AT5G41040.11.4e-4428.88HXXXD-type acyl-transferase family protein [more]
AT5G41040.21.4e-4428.88HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 3..453
e-value: 1.0E-104
score: 350.6
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 216..455
e-value: 1.2E-63
score: 216.2
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 3..210
e-value: 1.1E-61
score: 210.0
NoneNo IPR availablePANTHERPTHR31642:SF57SPERMIDINE HYDROXYCINNAMOYL TRANSFERASEcoord: 6..455
NoneNo IPR availablePANTHERPTHR31642TRICHOTHECENE 3-O-ACETYLTRANSFERASEcoord: 6..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G002540.1CmoCh03G002540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups