Cmc12g0338321 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0338321
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSNAP25 homologous protein SNAP33-like
LocationCMiso1.1chr12: 25993026 .. 25996569 (+)
RNA-Seq ExpressionCmc12g0338321
SyntenyCmc12g0338321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATGAAATTATATAACCTTTGAAGTTTGTAAATTTTTTGGTTGTTAAAAGTTTTTAAAAAATTACAAATTTAGTTCTAACTTTTCGAACCTTTAAAAAATTCTCACCCCTGTTGGGTTAGAAAATTGCAACTGATGGAACCTTCCGAGCGTTTGAAGTGAAGCAATTGAAAAGCAATCAAAAGTTGAATTTCACACTGAGTTTTATTATCTATCGCTCTTTAATTTTTGACCAAATCCATCGACATTATTTGATTTTTTTTTTTTTAATCCAAAAGGTCAATTTAGCGGTTCATGAGGTCAAGGTTGTCTTCTTCATTGTTCAACTTCTTTCTTCGCAGGTGCCCCTTTCAACAATTTCCTGATACGCGTTTTCTTAATTGATTTTTCTTCTCATTGCCCTAGTCTTCTATTGCCACTTTAAATTCTACAATTGGGTTCTGATTTTTGCAGTTTATTTGATTTTGGGTTCGTGAAATAGTTCCATTCTCTTCGTTTTCTGAACTCTCTCAAGGTACTCGAGTTTTTCTTTTCACTTTTTCTTTTGTTTATTGGATTTTTGTATCTGGGTTTTGATCACCATCTTCCTCCTTCGACTTTTACATGATTTTCTTAGTTAACTTGTTGTACCCTTTTAGTTTTTGATCGTGCCATTAACTGTTCTGATCTTACATCCATCTGTTTCTTCTTAATTAGATCAATTTGTATCTAACCTGTTTGATGTTTTACTTAAACAAAAATCTAAATCCTATAACATCTCATTGGTCGTTTTAACTTTAATGAAATTGATTGTTTTGATAAATTTGTCCCTTGTTGTATTTTGTTTCTTCTGCTGACATGATTGTGGTGCCTTGCTTTTCCAGGAAAAACCATTAAGTTATTGGATTACTTTGTTTCATTTACATTAAAGCCAATTTTGACATTCTAGCTCTCGGGAAAAATGTTTGGTTCAAAGAAGGTTCATTTTAGGATAGGAAAACATAGTTCTACCCCAAACCCTTTTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCATCTTCAAGTTCAATAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAATAGAAAACCAATCGGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAGGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAAGATGCTACTCAAACTTTGGTTGCATTACATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATTGATTACGATCTCAGTCGGGTATGTTCGAACTACGGTTTGTTCACTTTTGAAAATCTTGGTGTTCCATGGTTTAGGAAATTACTAGCATTTATTTTACATGTATAATTATAAGCTCTATCATTTATTTTTTAATTTGGTGAATTACTTTCCTGAACCTTTTTCCTTTCATGAATGCAATATCAGGGAGAGAAGCTGCTGGGAAATCTAGGAGGAATGTTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCGGGACCTATTATAAGCTCAGGTTGGTTTCTTTTCTTTGGGTTCACCATGATTGTCTCGCTCATGAGAATCCGAGTTAGATTAGCACTTGGTGAATTCTGTGCAACTGATCTGTTTATCTGCTTATGCATAATCTGTAGATGAAGTAAGAAGAAAGGGCAACCACTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGCGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTTTGTATTTGCTATCTATGAACAATTACTTAAAAGCAGTCGTTGACATTTAAACAGGCATGAATTATTGTATTTGCTTTTTGCTTAAGTATAGTCTTTGCACAATCCTGTACCATGTTGCCCAAAAACCATATTTATCATTCCCAAAGCGCTGTATCATTCATCTCACATAAGCTGTGTGTTTCAAAATTATGCATGAAACAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTACAGCTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGTTTGTTTCCATCTTGTATCTTCCAGAAAAACTTGGATCTTTCTTGCAAGTGAAAAACAAAAAGACTAGGTGTACATTGGAAATTGAGATGACCTGTTGTGCTTATTACAGGCAGAACGTTGCTCTGGATCGTGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAGAGTTCAAGGTCCTCACAGTTGCAGATTGGCCGACTGATGCTTATTTGCATCTTCCATTTTCTTGGCTTTGTATTCTTTTTCCTTTGAGAAATAGATATTGTGTGCCCCCTGTTCTTGATGACATTTTTTTTATTTGAGATCAATAAATACTTCATTAGTACTAGAATCGTCCATTTTCGAATACGGGAATTTTTAACGATCCAAGTTCTGATGGGTCTAAAGTGATATGAACAAAGATGCAACAATGCCATCTTTATAAAGAACTAATTTAAGCAAACAAGTATTCTTCATACTTTCAAGCTGTTATGAGCTCAAACAAGGTCAAATAAGTTCCTGGAGATGGGGCCAATCAATTCTAGCCCAGATACATACGAAACCATTATGGTATCCGCTAAATCATTAGTCTTGCGAGAGTTGCAAGAGGTTAAGATGTTGAAATGCCTAAAAGACAAAGTATCGAAGTAACAGGTTGCCATCCAAGTCAAAGATAGTGTTTAAAACCCAGAAGAGAAAATTCAGGAATGCATCAGACTCAAAGGGAAAAAATCCCTAATTTTACTTTCGAAATTAAGCTCGCAAGAGAATTTCAGGATGGGATGGAAAAAAATTTGCTCATAATTAATAACAACAGAATGTCAGTTTACAATTCAATTCTCACAAAAATTCTCAAATACTACAAATTCATCGAACCAAAAAAAAAAAAAAAACAAAAAGAAATTGAAATCAATGGTTGACATCCATGATGCTCTTGACCTTAGGCGCGTAAAGAGAGTAAACAAGGGCCTGGCGCTTGGCAACCTCGAGCTGAGCTCTGCCGCCAGCGTAGGCGGAGGAGATGGATGAAGCGTCGGAGAGACTCTGATTTTCTCGGAAAGAATCAATGGTACGGCGTTTGATGTATTCTCTGATGTTGTAATCAGGAAATTGACGGGCCGTTCGCAGGAGAGAACGAAAGAGCGAGAGGATCTCAGAGCTCGACGGAGTTGCCATCGGTGAAGGAGGAAGAGCCTAGGGATAGAGATTATAATGTACATATATATTATATATATGGATGTATGTATATTAATGTATATCCATTCTAATATATGAGAATGTACATGTATAGTACATACATTAGCTATGTACAAA

mRNA sequence

CTATGAAATTATATAACCTTTGAAGTTTGTAAATTTTTTGGTTGTTAAAAGTTTTTAAAAAATTACAAATTTAGTTCTAACTTTTCGAACCTTTAAAAAATTCTCACCCCTGTTGGGTTAGAAAATTGCAACTGATGGAACCTTCCGAGCGTTTGAAGTGAAGCAATTGAAAAGCAATCAAAAGTCAATTTAGCGGTTCATGAGGTCAAGGTTGTCTTCTTCATTGTTCAACTTCTTTCTTCGCAGTTTATTTGATTTTGGGTTCGTGAAATAGTTCCATTCTCTTCGTTTTCTGAACTCTCTCAAGGAAAAACCATTAAGTTATTGGATTACTTTGTTTCATTTACATTAAAGCCAATTTTGACATTCTAGCTCTCGGGAAAAATGTTTGGTTCAAAGAAGGTTCATTTTAGGATAGGAAAACATAGTTCTACCCCAAACCCTTTTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCATCTTCAAGTTCAATAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAATAGAAAACCAATCGGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAGGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAAGATGCTACTCAAACTTTGGTTGCATTACATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATTGATTACGATCTCAGTCGGGGAGAGAAGCTGCTGGGAAATCTAGGAGGAATGTTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCGGGACCTATTATAAGCTCAGATGAAGTAAGAAGAAAGGGCAACCACTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGCGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTACAGCTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGCAGAACGTTGCTCTGGATCGTGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAGAGTTCAAGGTCCTCACAGTTGCAGATTGGCCGACTGATGCTTATTTGCATCTTCCATTTTCTTGGCTTTGTATTCTTTTTCCTTTGAGAAATAGATATTGTGTGCCCCCTGTTCTTGATGACATTTTTTTTATTTGAGATCAATAAATACTTCATTAGTACTAGAATCGTCCATTTTCGAATACGGGAATTTTTAACGATCCAAGTTCTGATGGGTCTAAAGTGATATGAACAAAGATGCAACAATGCCATCTTTATAAAGAACTAATTTAAGCAAACAAGTATTCTTCATACTTTCAAGCTGTTATGAGCTCAAACAAGGTCAAATAAGTTCCTGGAGATGGGGCCAATCAATTCTAGCCCAGATACATACGAAACCATTATGGTATCCGCTAAATCATTAGTCTTGCGAGAGTTGCAAGAGGTTAAGATGTTGAAATGCCTAAAAGACAAAGTATCGAAGTAACAGGTTGCCATCCAAGTCAAAGATAGTGTTTAAAACCCAGAAGAGAAAATTCAGGAATGCATCAGACTCAAAGGGAAAAAATCCCTAATTTTACTTTCGAAATTAAGCTCGCAAGAGAATTTCAGGATGGGATGGAAAAAAATTTGCTCATAATTAATAACAACAGAATGTCAGTTTACAATTCAATTCTCACAAAAATTCTCAAATACTACAAATTCATCGAACCAAAAAAAAAAAAAAAACAAAAAGAAATTGAAATCAATGGTTGACATCCATGATGCTCTTGACCTTAGGCGCGTAAAGAGAGTAAACAAGGGCCTGGCGCTTGGCAACCTCGAGCTGAGCTCTGCCGCCAGCGTAGGCGGAGGAGATGGATGAAGCGTCGGAGAGACTCTGATTTTCTCGGAAAGAATCAATGGTACGGCGTTTGATGTATTCTCTGATGTTGTAATCAGGAAATTGACGGGCCGTTCGCAGGAGAGAACGAAAGAGCGAGAGGATCTCAGAGCTCGACGGAGTTGCCATCGGTGAAGGAGGAAGAGCCTAGGGATAGAGATTATAATGTACATATATATTATATATATGGATGTATGTATATTAATGTATATCCATTCTAATATATGAGAATGTACATGTATAGTACATACATTAGCTATGTACAAA

Coding sequence (CDS)

ATGTTTGGTTCAAAGAAGGTTCATTTTAGGATAGGAAAACATAGTTCTACCCCAAACCCTTTTGAGTCTGATGATGATGTTAAAGCTAAGCAAAACCTGGTTTCTTCACAAAGAACTTCCTCAGACCCTTCATTACTGACGGAGAAGTTTAGTTCCAATCCTTTTGATGATGATCATGGACATGCCCCCTCATCTTCAAGTTCAATAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTTCGTGACTCAGGAGGAATAGAAAACCAATCGGTACAGGAACTGGAGAATTATGCTGTTTATAAAGCTGAGGAGACAACAAAAGCAGTGAATGGCTGTTTAAAGATTGCTGAAGAGATAAGGGAAGATGCTACTCAAACTTTGGTTGCATTACATCACCAGGGTGAGCAAATTACCAGGACTCATATGGTTGCTGCTGACATTGATTACGATCTCAGTCGGGGAGAGAAGCTGCTGGGAAATCTAGGAGGAATGTTCTCTAAGACTTGGAAGCCAAAGAAAACTCGACCAATCTCGGGACCTATTATAAGCTCAGATGAAGTAAGAAGAAAGGGCAACCACTTAGAGCAGAAGGAAAGGTTAGGATTGACTCATCATAAGGAGCGGACCAGTACAAAAGCTGCATTTGCTGAACCTACCAATGCAATTCAAAAAGTTGAGGTAGAAAAGATGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTACAGCTGAAGGAAATGGCTGTTGACATGGGTAGTGAAATGGACAGGCAGAACGTTGCTCTGGATCGTGCTTCTGTTGATGTTGAGGTGCTTGGTGACCGAGTTAAAGATGCCAACCGACGCGGTCGTCGTTTGCTTGGAAAATAG

Protein sequence

MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISGPIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK
Homology
BLAST of Cmc12g0338321 vs. NCBI nr
Match: XP_008447972.1 (PREDICTED: SNAP25 homologous protein SNAP33-like [Cucumis melo] >TYJ98921.1 SNAP25-like proteinous protein SNAP33-like [Cucumis melo var. makuwa])

HSP 1 Score: 565.8 bits (1457), Expect = 2.1e-157
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240
           PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM
Sbjct: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240

Query: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

BLAST of Cmc12g0338321 vs. NCBI nr
Match: XP_004137424.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >XP_031743143.1 SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN64035.1 hypothetical protein Csa_013597 [Cucumis sativus])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+KVHFR+GKHSSTPNP ESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSD+ VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILVQLKEMAVDMGSEMDRQNVALD ASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 293

BLAST of Cmc12g0338321 vs. NCBI nr
Match: XP_038894894.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 523.9 bits (1348), Expect = 9.1e-145
Identity = 270/293 (92.15%), Postives = 281/293 (95.90%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+K HFRIGKHSSTPNPFESDD VKA+QN VSSQRTSSDPSLLTE FSSNPFDDDHG
Sbjct: 1   MFGSRKAHFRIGKHSSTPNPFESDDGVKAQQNQVSSQRTSSDPSLLTENFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS+TWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLV LHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVTLHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PII+SD+ VRRKGNHLEQKERLGLTHHK RT+TK AFAEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PIINSDDLVRRKGNHLEQKERLGLTHHKGRTNTKTAFAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN  LDRAS DVEVLGDRVKDANRRGRRLLG+
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNNVLDRASDDVEVLGDRVKDANRRGRRLLGR 293

BLAST of Cmc12g0338321 vs. NCBI nr
Match: XP_023520322.1 (SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.1 bits (1307), Expect = 5.2e-140
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+K H RIGKHSSTPNPFESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDDDHG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSS S+TWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS++ VRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQK EVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALD ASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

BLAST of Cmc12g0338321 vs. NCBI nr
Match: XP_023523341.1 (SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo] >XP_023523342.1 SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 3.4e-139
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+K H +IG+HSSTPNPFESDD  KAKQNLV+SQRTSSDPSL+ E F SNPFDDDHG
Sbjct: 1   MFGSRKAHLKIGRHSSTPNPFESDDGFKAKQNLVTSQRTSSDPSLVKESFISNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS+TWSERNKYKNDFRDSGG+ENQSVQELENYA YKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGLENQSVQELENYATYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALH QGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHQQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PI++SD+ VRRKG+HLEQKE+LGLTHHK RT+TKAAFAEPTNAIQKVEVEK+KQDDALSD
Sbjct: 181 PIVNSDDLVRRKGSHLEQKEKLGLTHHKGRTNTKAAFAEPTNAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MS+IL +LKEMAVDMG E+DRQ VALD ASVDVEVLGDRVKDA+RRGRRLLGK
Sbjct: 241 MSNILEELKEMAVDMGCEIDRQIVALDHASVDVEVLGDRVKDASRRGRRLLGK 293

BLAST of Cmc12g0338321 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.2e-84
Identity = 176/303 (58.09%), Postives = 224/303 (73.93%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKH------SSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNP 60
           MFG +K    + KH      SS PNPF+SDD+   K  L  S+RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLAD---MTNP 60

Query: 61  FDDDH---GHAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVN 120
           F  +    G + SS  S+  + + +YKN+FRDSGGIENQSVQELE YAVYKAEETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQITRTH  A +ID+DLSRGEKLLG+LGGMFSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPIISSDE-VRRKGNHLEQKERLGL-THHKERTSTKAAFAEPTNAIQKVEVE 240
           PKKTRPI+GP+++ D+   R+ NHLE++E+LGL +  + ++ T+    E  +A Q+VE+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRL 293
           K KQDD LSD+SDIL +LK MAVDMGSE+++QN  LD    DV+ L  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

BLAST of Cmc12g0338321 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.6e-70
Identity = 150/261 (57.47%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 33  LVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSITWSERNKYKNDFRDSGGIENQSV 92
           + + +RTSS+P L+T       FDDD                +KYKN F DSGG+++Q+ 
Sbjct: 25  ITAGRRTSSEPILITP-----DFDDD----------------DKYKNGFNDSGGLQSQTT 84

Query: 93  QELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDL 152
           +ELE YAVYKAEETTK VN CLKIAE+IR D  +TL  LH QGEQI RTH +A D+D DL
Sbjct: 85  EELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDL 144

Query: 153 SRGEKLLGNLGGMFSKTWKPKKTRPISGPIISSDEVRRKG-NHLEQKERLGLTHHKERTS 212
           SRGEKLL NLGGMFSK WKPKKT+ I+GP+I+ D+  +K  NH E++E+LGL   K R+S
Sbjct: 145 SRGEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEEREKLGL-GAKGRSS 204

Query: 213 TKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVD 272
           ++ A  +PTNA+QKVE EK KQDD LSD+SDIL  LK MAVDMGSE+D+QN ALD    D
Sbjct: 205 SQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDD 263

Query: 273 VEVLGDRVKDANRRGRRLLGK 293
           V+ L  RV+ AN+R R LL K
Sbjct: 265 VDELNSRVQGANQRARHLLSK 263

BLAST of Cmc12g0338321 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-52
Identity = 124/259 (47.88%), Postives = 166/259 (64.09%), Query Frame = 0

Query: 35  SSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSITWSERNKYKNDFRDSGGIENQSVQE 94
           SS     D     + FS NPFDDD                 ++ +  + SGG ENQ+VQE
Sbjct: 6   SSWNPFDDEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQE 65

Query: 95  LENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSR 154
           LE+YAVY +EETTK V GCLK+AEEIR DA++TLV L+ QG+QITRTH    D+D+ LSR
Sbjct: 66  LESYAVYNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSR 125

Query: 155 GEKLLGNLGGMFSKTWKPKKTRPISGPIIS-SDEVRRKGNHLEQKERLGLTHHKERTSTK 214
           GEK+LG LGG+FS+TWKPKK+R I+GP+I+  D  +RK   L+ +E+LGL    +  S  
Sbjct: 126 GEKILGRLGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKS-- 185

Query: 215 AAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVDVE 274
               E  +A QK ++   KQD+AL+D+S +L +LK MAVDMG+ ++RQ   LD    + +
Sbjct: 186 KTLPEAVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNAD 245

Query: 275 VLGDRVKDANRRGRRLLGK 293
            L  RVK +N+R R LL K
Sbjct: 246 ELNYRVKQSNQRARYLLRK 251

BLAST of Cmc12g0338321 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-09
Identity = 55/206 (26.70%), Postives = 101/206 (49.03%), Query Frame = 0

Query: 92  VQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYD 151
           ++E++  A   A+E+ ++    L++ EE ++   +TLV L  QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGMFSKTWKPKKTRPISGPIISSDEVRRK-GNHLE---QKERLGLTHHK 211
           +   EK L +LG        P         + SSD  ++  GN+ +     +   +   +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 ERTSTKAAFAEPTNAIQKV--EVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVAL 271
           E+ +    F      I++V  +  + + D+ L  +S I+  L+ MA+DMG+E+D QN  +
Sbjct: 131 EQMAISGGF------IRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQI 190

Query: 272 DRASVDVEVLGDRVKDANRRGRRLLG 292
           DR     +    R+ +AN+R  ++LG
Sbjct: 191 DRIMEKADSNKTRIDEANQRATKMLG 204

BLAST of Cmc12g0338321 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-09
Identity = 55/206 (26.70%), Postives = 101/206 (49.03%), Query Frame = 0

Query: 92  VQELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYD 151
           ++E++  A   A+E+ ++    L++ EE ++   +TLV L  QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGMFSKTWKPKKTRPISGPIISSDEVRRK-GNHLE---QKERLGLTHHK 211
           +   EK L +LG        P         + SSD  ++  GN+ +     +   +   +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 ERTSTKAAFAEPTNAIQKV--EVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVAL 271
           E+ +    F      I++V  +  + + D+ L  +S I+  L+ MA+DMG+E+D QN  +
Sbjct: 131 EQMAISGGF------IRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQI 190

Query: 272 DRASVDVEVLGDRVKDANRRGRRLLG 292
           DR     +    R+ +AN+R  ++LG
Sbjct: 191 DRIMEKADSNKTRIDEANQRATKMLG 204

BLAST of Cmc12g0338321 vs. ExPASy TrEMBL
Match: A0A5D3BJM2 (SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001190 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.0e-157
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240
           PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM
Sbjct: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240

Query: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

BLAST of Cmc12g0338321 vs. ExPASy TrEMBL
Match: A0A1S3BI33 (SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.0e-157
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240
           PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM
Sbjct: 181 PIISSDEVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSDM 240

Query: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 SDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

BLAST of Cmc12g0338321 vs. ExPASy TrEMBL
Match: A0A0A0LQ81 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039020 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 5.8e-153
Identity = 284/293 (96.93%), Postives = 289/293 (98.63%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+KVHFR+GKHSSTPNP ESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG
Sbjct: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS TWSERNKYKNDFRDSGG+ENQSVQELENYAVYKAEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           PIISSD+ VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD
Sbjct: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILVQLKEMAVDMGSEMDRQNVALD ASVDVEVLGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 293

BLAST of Cmc12g0338321 vs. ExPASy TrEMBL
Match: A0A6J1E891 (SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431714 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.1e-139
Identity = 258/293 (88.05%), Postives = 277/293 (94.54%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+K H RIGKHSSTPNPFESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDD HG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDHHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
            APSSS S+TWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  QAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS++ VRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALD ASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

BLAST of Cmc12g0338321 vs. ExPASy TrEMBL
Match: A0A6J1KE92 (SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC111495025 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.8e-139
Identity = 258/293 (88.05%), Postives = 277/293 (94.54%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60
           MFGS+K H RIGKH STPNPFESDD VKAKQN VS+QRTSSDPSL+ E F SNPFDDDHG
Sbjct: 1   MFGSRKGHLRIGKHGSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVAENFGSNPFDDDHG 60

Query: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120
           HAPSSSSS+TWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQITRTH+VAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQITRTHVVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240
           P+ISS++ VRRKGNH+EQKE+LGLTHHK RT+TKAA+AEPT+AIQKVEVEK+KQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTHHKGRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALD ASVDVEV+GDRVKDANRRG RLL K
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGHRLLRK 293

BLAST of Cmc12g0338321 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 312.8 bits (800), Expect = 3.0e-85
Identity = 176/303 (58.09%), Postives = 224/303 (73.93%), Query Frame = 0

Query: 1   MFGSKKVHFRIGKH------SSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNP 60
           MFG +K    + KH      SS PNPF+SDD+   K  L  S+RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLAD---MTNP 60

Query: 61  FDDDH---GHAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVN 120
           F  +    G + SS  S+  + + +YKN+FRDSGGIENQSVQELE YAVYKAEETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQITRTH  A +ID+DLSRGEKLLG+LGGMFSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPIISSDE-VRRKGNHLEQKERLGL-THHKERTSTKAAFAEPTNAIQKVEVE 240
           PKKTRPI+GP+++ D+   R+ NHLE++E+LGL +  + ++ T+    E  +A Q+VE+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRL 293
           K KQDD LSD+SDIL +LK MAVDMGSE+++QN  LD    DV+ L  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

BLAST of Cmc12g0338321 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 266.9 bits (681), Expect = 1.9e-71
Identity = 150/261 (57.47%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 33  LVSSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSITWSERNKYKNDFRDSGGIENQSV 92
           + + +RTSS+P L+T       FDDD                +KYKN F DSGG+++Q+ 
Sbjct: 25  ITAGRRTSSEPILITP-----DFDDD----------------DKYKNGFNDSGGLQSQTT 84

Query: 93  QELENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDL 152
           +ELE YAVYKAEETTK VN CLKIAE+IR D  +TL  LH QGEQI RTH +A D+D DL
Sbjct: 85  EELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDL 144

Query: 153 SRGEKLLGNLGGMFSKTWKPKKTRPISGPIISSDEVRRKG-NHLEQKERLGLTHHKERTS 212
           SRGEKLL NLGGMFSK WKPKKT+ I+GP+I+ D+  +K  NH E++E+LGL   K R+S
Sbjct: 145 SRGEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEEREKLGL-GAKGRSS 204

Query: 213 TKAAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVD 272
           ++ A  +PTNA+QKVE EK KQDD LSD+SDIL  LK MAVDMGSE+D+QN ALD    D
Sbjct: 205 SQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDD 263

Query: 273 VEVLGDRVKDANRRGRRLLGK 293
           V+ L  RV+ AN+R R LL K
Sbjct: 265 VDELNSRVQGANQRARHLLSK 263

BLAST of Cmc12g0338321 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 208.4 bits (529), Expect = 7.9e-54
Identity = 124/259 (47.88%), Postives = 166/259 (64.09%), Query Frame = 0

Query: 35  SSQRTSSDPSLLTEKFSSNPFDDDHGHAPSSSSSITWSERNKYKNDFRDSGGIENQSVQE 94
           SS     D     + FS NPFDDD                 ++ +  + SGG ENQ+VQE
Sbjct: 6   SSWNPFDDEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQE 65

Query: 95  LENYAVYKAEETTKAVNGCLKIAEEIREDATQTLVALHHQGEQITRTHMVAADIDYDLSR 154
           LE+YAVY +EETTK V GCLK+AEEIR DA++TLV L+ QG+QITRTH    D+D+ LSR
Sbjct: 66  LESYAVYNSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSR 125

Query: 155 GEKLLGNLGGMFSKTWKPKKTRPISGPIIS-SDEVRRKGNHLEQKERLGLTHHKERTSTK 214
           GEK+LG LGG+FS+TWKPKK+R I+GP+I+  D  +RK   L+ +E+LGL    +  S  
Sbjct: 126 GEKILGRLGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKS-- 185

Query: 215 AAFAEPTNAIQKVEVEKMKQDDALSDMSDILVQLKEMAVDMGSEMDRQNVALDRASVDVE 274
               E  +A QK ++   KQD+AL+D+S +L +LK MAVDMG+ ++RQ   LD    + +
Sbjct: 186 KTLPEAVDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNAD 245

Query: 275 VLGDRVKDANRRGRRLLGK 293
            L  RVK +N+R R LL K
Sbjct: 246 ELNYRVKQSNQRARYLLRK 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008447972.12.1e-157100.00PREDICTED: SNAP25 homologous protein SNAP33-like [Cucumis melo] >TYJ98921.1 SNAP... [more]
XP_004137424.11.2e-15296.93SNAP25 homologous protein SNAP33 [Cucumis sativus] >XP_031743143.1 SNAP25 homolo... [more]
XP_038894894.19.1e-14592.15SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25... [more]
XP_023520322.15.2e-14088.40SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo][more]
XP_023523341.13.4e-13988.40SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo] >XP_023523342... [more]
Match NameE-valueIdentityDescription
Q9S7P94.2e-8458.09SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ... [more]
Q9LMG82.6e-7057.47Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA... [more]
Q9SD961.1e-5247.88SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ... [more]
Q17QQ31.5e-0926.70Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1[more]
P608781.5e-0926.70Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BJM21.0e-157100.00SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BI331.0e-157100.00SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE... [more]
A0A0A0LQ815.8e-15396.93t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A6J1E8912.1e-13988.05SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431... [more]
A0A6J1KE922.8e-13988.05SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC11149502... [more]
Match NameE-valueIdentityDescription
AT5G61210.13.0e-8558.09soluble N-ethylmaleimide-sensitive factor adaptor protein 33 [more]
AT1G13890.11.9e-7157.47soluble N-ethylmaleimide-sensitive factor adaptor protein 30 [more]
AT5G07880.17.9e-5447.88synaptosomal-associated protein SNAP25-like 29 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 222..289
e-value: 0.22
score: 20.6
coord: 95..162
e-value: 2.4
score: 11.3
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 227..289
score: 13.320502
NoneNo IPR availableGENE3D1.20.5.110coord: 86..164
e-value: 2.0E-19
score: 71.3
NoneNo IPR availableGENE3D1.20.5.110coord: 224..291
e-value: 2.7E-18
score: 68.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..51
NoneNo IPR availablePANTHERPTHR19305SYNAPTOSOMAL ASSOCIATED PROTEINcoord: 1..292
NoneNo IPR availablePANTHERPTHR19305:SF30TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 1..292
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 232..288
e-value: 2.82917E-12
score: 58.724
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 90..160
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 227..290
IPR044766NPSN/SNAP25-like, N-terminal SNARE domainCDDcd15861SNARE_SNAP25N_23N_29N_SEC9Ncoord: 97..161
e-value: 2.33463E-19
score: 77.9987

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0338321.1Cmc12g0338321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061025 membrane fusion
biological_process GO:0015031 protein transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005484 SNAP receptor activity