Cmc12g0330981 (gene) Melon (Charmono) v1.1

Overview
NameCmc12g0330981
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionnon-classical arabinogalactan protein 31
LocationCMiso1.1chr12: 21841095 .. 21842260 (-)
RNA-Seq ExpressionCmc12g0330981
SyntenyCmc12g0330981
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAACCATCACCCATAGTATATATAATAAACCCCTTGCGTCCAATGCTTCAATATTCTTTCGTATCACTACCAAATGGCCTCTCTTACTGCCAATGCTTCTTTCCTTGTCCTCCTCCTCCTCTGCTGCAGCCTCAATGCTTTCCAAGCCGCCGCATATTCCTCCACCCCAGCTCCGGCACCCACTCACCACAACGCCCACCACCCTGTAGCTGCCCCAGCTCCAAGCTTCCACCACCACGGCCACCACCACCACCACCACTCCCCGAGCCAGTCTCCGACTTCCCATCACCATCCTCATTCCCCTTCCCCTGCTCCTTCCCCTGTTTACCCATTTCCCCCTACGGCTCACTACGCCCCGGTTCCCTCTCCGGTTCCCTCACCAGTTCATTCACCTAAACCGTCCACCTACGTCCCAAGAAGCTTCGTTGAAGTCCAAGGCGTTGTTTATTGCAAGTCTTGCAAGTACCCCGGCGTTGATACTCTTCTCGGAGCTAAACCACTTTCCGGTAAGTTCCAAGATCGCTTTTTTACTCAAAAACCATTATATTTACAGTAAACCCACATAAGATTTAGCGGTACTTTTACTAATTTTTCAAAAATATTTGAAATGGGTATTGCGAAATGGCCTTAGTTGCTCTGTTTTTCTTTTTTCTTTCAGGCGCTACAGTGAAGTTGTCATGCAAGAACACCAAGTACGCTCCGACGGTGGAAACCGCCACCAGCGACAAGAATGGGTACTTCCGGTTAGCGGCGCCGAAGAATGTGACGAGCTACGCATTCCACCGATGCAAGGTGTATTTAGTGGAGTCGCCGGATAGCAATTGCAAGAAAGCGTCGAAACTGAACGGGGGAGAGGATGGAGCGGAGTTAAAGCCGGCGAGAGCATTCACGGACGAGGAGAAGAAGCCGGTTGTGCTTTACAACGTTGGTCCATTGGCATTTGAACCAACCTGTAGTGGTTGAAAGTAGGGGGGAAGGAGAGAGGGGTAGTTTAGTAATATGTGGGGGTTTTGTATTGGTAATGACTTATGATATATGCCTTTGGATTCCTGCACTTTTTCTAGGGTATTGTGACATTGTTGTTTTTGTGAAATCCTTTATATGGAGTCGAGTTTTTATTCTATTTCGTACCCCTTCTTTCTAAAAGAAAAAATAAATCTTTAAC

mRNA sequence

TTAACCATCACCCATAGTATATATAATAAACCCCTTGCGTCCAATGCTTCAATATTCTTTCGTATCACTACCAAATGGCCTCTCTTACTGCCAATGCTTCTTTCCTTGTCCTCCTCCTCCTCTGCTGCAGCCTCAATGCTTTCCAAGCCGCCGCATATTCCTCCACCCCAGCTCCGGCACCCACTCACCACAACGCCCACCACCCTGTAGCTGCCCCAGCTCCAAGCTTCCACCACCACGGCCACCACCACCACCACCACTCCCCGAGCCAGTCTCCGACTTCCCATCACCATCCTCATTCCCCTTCCCCTGCTCCTTCCCCTGTTTACCCATTTCCCCCTACGGCTCACTACGCCCCGGTTCCCTCTCCGGTTCCCTCACCAGTTCATTCACCTAAACCGTCCACCTACGTCCCAAGAAGCTTCGTTGAAGTCCAAGGCGTTGTTTATTGCAAGTCTTGCAAGTACCCCGGCGTTGATACTCTTCTCGGAGCTAAACCACTTTCCGGCGCTACAGTGAAGTTGTCATGCAAGAACACCAAGTACGCTCCGACGGTGGAAACCGCCACCAGCGACAAGAATGGGTACTTCCGGTTAGCGGCGCCGAAGAATGTGACGAGCTACGCATTCCACCGATGCAAGGTGTATTTAGTGGAGTCGCCGGATAGCAATTGCAAGAAAGCGTCGAAACTGAACGGGGGAGAGGATGGAGCGGAGTTAAAGCCGGCGAGAGCATTCACGGACGAGGAGAAGAAGCCGGTTGTGCTTTACAACGTTGGTCCATTGGCATTTGAACCAACCTGTAGTGGTTGAAAGTAGGGGGGAAGGAGAGAGGGGTAGTTTAGTAATATGTGGGGGTTTTGTATTGGTAATGACTTATGATATATGCCTTTGGATTCCTGCACTTTTTCTAGGGTATTGTGACATTGTTGTTTTTGTGAAATCCTTTATATGGAGTCGAGTTTTTATTCTATTTCGTACCCCTTCTTTCTAAAAGAAAAAATAAATCTTTAAC

Coding sequence (CDS)

ATGGCCTCTCTTACTGCCAATGCTTCTTTCCTTGTCCTCCTCCTCCTCTGCTGCAGCCTCAATGCTTTCCAAGCCGCCGCATATTCCTCCACCCCAGCTCCGGCACCCACTCACCACAACGCCCACCACCCTGTAGCTGCCCCAGCTCCAAGCTTCCACCACCACGGCCACCACCACCACCACCACTCCCCGAGCCAGTCTCCGACTTCCCATCACCATCCTCATTCCCCTTCCCCTGCTCCTTCCCCTGTTTACCCATTTCCCCCTACGGCTCACTACGCCCCGGTTCCCTCTCCGGTTCCCTCACCAGTTCATTCACCTAAACCGTCCACCTACGTCCCAAGAAGCTTCGTTGAAGTCCAAGGCGTTGTTTATTGCAAGTCTTGCAAGTACCCCGGCGTTGATACTCTTCTCGGAGCTAAACCACTTTCCGGCGCTACAGTGAAGTTGTCATGCAAGAACACCAAGTACGCTCCGACGGTGGAAACCGCCACCAGCGACAAGAATGGGTACTTCCGGTTAGCGGCGCCGAAGAATGTGACGAGCTACGCATTCCACCGATGCAAGGTGTATTTAGTGGAGTCGCCGGATAGCAATTGCAAGAAAGCGTCGAAACTGAACGGGGGAGAGGATGGAGCGGAGTTAAAGCCGGCGAGAGCATTCACGGACGAGGAGAAGAAGCCGGTTGTGCTTTACAACGTTGGTCCATTGGCATTTGAACCAACCTGTAGTGGTTGA

Protein sequence

MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHHHHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEVQGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFEPTCSG
Homology
BLAST of Cmc12g0330981 vs. NCBI nr
Match: XP_008442660.1 (PREDICTED: non-classical arabinogalactan protein 31 [Cucumis melo] >KAA0056996.1 non-classical arabinogalactan protein 31 [Cucumis melo var. makuwa] >TYK26423.1 non-classical arabinogalactan protein 31 [Cucumis melo var. makuwa])

HSP 1 Score: 482.3 bits (1240), Expect = 2.6e-132
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH
Sbjct: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV
Sbjct: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240

Query: 241 PTCSG 246
           PTCSG
Sbjct: 241 PTCSG 245

BLAST of Cmc12g0330981 vs. NCBI nr
Match: XP_004146606.2 (non-classical arabinogalactan protein 31 [Cucumis sativus] >KGN64686.1 hypothetical protein Csa_013639 [Cucumis sativus])

HSP 1 Score: 422.9 bits (1086), Expect = 1.8e-114
Identity = 218/244 (89.34%), Postives = 226/244 (92.62%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MASLTANASFL+LLL C  LNAFQAAAYSSTPAPAPTHHNAHHPVAAP PSFHH GHHHH
Sbjct: 1   MASLTANASFLLLLLCCTLLNAFQAAAYSSTPAPAPTHHNAHHPVAAPTPSFHHRGHHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HHSP+QSPTSHHHPHSPSPAPSPVYP  P AHYA    PVPSP HSPKPST +PRSFV+V
Sbjct: 61  HHSPTQSPTSHHHPHSPSPAPSPVYPLHPPAHYA----PVPSPAHSPKPSTNIPRSFVQV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYP VDTLLGAKPLSGATVKLSCKNTKYAP VETATSD+NGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPDVDTLLGAKPLSGATVKLSCKNTKYAPAVETATSDENGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS C+KASK+NGG DGAELKPARAFTDEEKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSKCEKASKMNGGVDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240

Query: 241 PTCS 245
           PTCS
Sbjct: 241 PTCS 240

BLAST of Cmc12g0330981 vs. NCBI nr
Match: XP_038876988.1 (non-classical arabinogalactan protein 31 [Benincasa hispida])

HSP 1 Score: 412.9 bits (1060), Expect = 1.9e-111
Identity = 217/243 (89.30%), Postives = 224/243 (92.18%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MAS+TANASFLV LLLCCS+NAFQ AAY  TPAPAP+HH  HHPVAAP PSFHHH  HH 
Sbjct: 1   MASVTANASFLV-LLLCCSINAFQTAAYHGTPAPAPSHHGGHHPVAAPTPSFHHH-RHHR 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HH+PS SP SHHHPH  SPAPSPVYP PPTAHYAPVPSPVPSPV   KPSTYVPRSFVEV
Sbjct: 61  HHAPSPSPISHHHPH--SPAPSPVYPSPPTAHYAPVPSPVPSPVPPSKPSTYVPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS+C KASKLNGGEDGAELKPARAFTD+EKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSSCSKASKLNGGEDGAELKPARAFTDDEKKPVVLYNVGPLAFE 239

Query: 241 PTC 244
           PTC
Sbjct: 241 PTC 239

BLAST of Cmc12g0330981 vs. NCBI nr
Match: KAG7019613.1 (Non-classical arabinogalactan protein 31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.8 bits (878), Expect = 2.4e-90
Identity = 187/244 (76.64%), Postives = 203/244 (83.20%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           M S+   A  +++LLL C+LNAFQAAA   +P  AP   + H+PV   APS HHH HHHH
Sbjct: 1   MGSVATGA--VIVLLLGCALNAFQAAASHGSPTLAPRPDDRHYPVPVAAPSHHHH-HHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HH PSQSP  HHH H  SPAPSPVY  PP AHYA    PVPSPV  PK STY+PRSFVEV
Sbjct: 61  HHPPSQSPIYHHHSH--SPAPSPVYAPPPPAHYA----PVPSPVQPPKRSTYIPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSC YPGVDTLLGAKPL+GATVKLSCKNTKYAPTVETAT+DKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCHYPGVDTLLGAKPLNGATVKLSCKNTKYAPTVETATTDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS+C K SK+NGGEDGAELKPA+AFTD EKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSSCNKMSKMNGGEDGAELKPAKAFTDAEKKPVVLYNVGPLAFE 235

Query: 241 PTCS 245
           PTC+
Sbjct: 241 PTCA 235

BLAST of Cmc12g0330981 vs. NCBI nr
Match: XP_022927607.1 (non-classical arabinogalactan protein 31-like [Cucurbita moschata])

HSP 1 Score: 341.7 bits (875), Expect = 5.4e-90
Identity = 186/244 (76.23%), Postives = 203/244 (83.20%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           M S+   A  +++LLL C+LNAFQAAA   +P  AP   + H+PV   APS HHH HHHH
Sbjct: 1   MGSVATGA--VIVLLLGCALNAFQAAASHGSPTLAPRPDDRHYPVPVAAPSHHHH-HHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HH PSQSP  HHH H  SPAPSPVY  PP AHYA    PVPSPV  PK STY+PRSFVEV
Sbjct: 61  HHPPSQSPIYHHHSH--SPAPSPVYAPPPPAHYA----PVPSPVQPPKRSTYIPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSC YPGVDTLLGAKPL+GATVKLSCKNTKYAPT+ETAT+DKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCHYPGVDTLLGAKPLNGATVKLSCKNTKYAPTMETATTDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS+C K SK+NGGEDGAELKPA+AFTD EKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSSCNKMSKMNGGEDGAELKPAKAFTDAEKKPVVLYNVGPLAFE 235

Query: 241 PTCS 245
           PTC+
Sbjct: 241 PTCA 235

BLAST of Cmc12g0330981 vs. ExPASy Swiss-Prot
Match: Q9FZA2 (Non-classical arabinogalactan protein 31 OS=Arabidopsis thaliana OX=3702 GN=AGP31 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.5e-31
Identity = 109/230 (47.39%), Postives = 125/230 (54.35%), Query Frame = 0

Query: 28  YSSTPAPA--PTHHNAHHPVAAPAPSFHHHGHHHHHHSPSQSPTSH--HHPHSPSPAPSP 87
           Y  T AP   PT      PV  P              +P + PT      P SP PA  P
Sbjct: 145 YPPTKAPVKPPTKPPVKPPVYPPT------------KAPVKPPTKPPVKPPVSP-PAKPP 204

Query: 88  VYP--FPPTAHYAPVPSPVPSPVHSP-KPSTYVP---RSFVEVQGVVYCKSCKYPGVDTL 147
           V P  +PPT   APV  PV  P   P  P  Y P   RS V V+G VYCKSCKY   +TL
Sbjct: 205 VKPPVYPPTK--APVKPPVSPPTKPPVTPPVYPPKFNRSLVAVRGTVYCKSCKYAAFNTL 264

Query: 148 LGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFHRCKVYLVESPD 207
           LGAKP+ GATVKL CK+ K   T ET T+DKNGYF L APK VT++ F  C+VYLV+S D
Sbjct: 265 LGAKPIEGATVKLVCKSKKNI-TAET-TTDKNGYFLLLAPKTVTNFGFRGCRVYLVKSKD 324

Query: 208 SNCKKASKLNGGEDGAELKPARAFTDE----EKKPVVLYNVGPLAFEPTC 244
             C K SKL GG+ GAELKP +          K    L+NVGP AF P+C
Sbjct: 325 YKCSKVSKLFGGDVGAELKPEKKLGKSTVVVNKLVYGLFNVGPFAFNPSC 357

BLAST of Cmc12g0330981 vs. ExPASy Swiss-Prot
Match: P93013 (Non-classical arabinogalactan protein 30 OS=Arabidopsis thaliana OX=3702 GN=AGP30 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.7e-23
Identity = 91/254 (35.83%), Postives = 126/254 (49.61%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAY---SSTPAPAPTHHNAHHPVAAPAPSFHHHGH 60
           M  +  + S  +  LLC + + F        SS P  +   H    P+  P         
Sbjct: 1   MGIIGKSVSLTLFALLCFTSSVFTLGVNQPGSSDPFHSLPQHLPLPPIKLPT-------- 60

Query: 61  HHHHHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSP-KPSTYVP-- 120
                 P+++P      + P+ AP  +   PP    AP+  P   P+  P  P  Y P  
Sbjct: 61  ----LPPAKAPIK-LPAYPPAKAPIKLPTLPPAK--APIKLPTLPPIKPPVLPPVYPPKY 120

Query: 121 -RSFVEVQGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFR 180
            ++ V V+GVVYCK+CKY GV+ + GAKP+  A V+L CKN K   ++    +DKNGYF 
Sbjct: 121 NKTLVAVRGVVYCKACKYAGVNNVQGAKPVKDAVVRLVCKNKK--NSISETKTDKNGYFM 180

Query: 181 LAAPKNVTSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPAR----AFTDEEKKPV 240
           L APK VT+Y    C+ +LV+SPD+ C K S L+ G  G+ LKP      + T       
Sbjct: 181 LLAPKTVTNYDIKGCRAFLVKSPDTKCSKVSSLHDGGKGSVLKPVLKPGFSSTIMRWFKY 237

Query: 241 VLYNVGPLAFEPTC 244
            +YNVGP AFEPTC
Sbjct: 241 SVYNVGPFAFEPTC 237

BLAST of Cmc12g0330981 vs. ExPASy Swiss-Prot
Match: Q03211 (Pistil-specific extensin-like protein OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.0e-18
Identity = 88/242 (36.36%), Postives = 110/242 (45.45%), Query Frame = 0

Query: 31  TPAPAPTHHNAHHPVAAPAPSFHHHGHHHHHHSPSQSPTSHHHPHSPSPAPSPVYPFPPT 90
           +P+PA        PV AP+P            SP+  P +   P  P    SP+ P PP 
Sbjct: 190 SPSPAKQPPPPPPPVKAPSP------------SPATQPPTKQPPPPPRAKKSPLLPPPPP 249

Query: 91  AHYAPVPSPVPS-----PVHSPKPS-----TYVPRSFVE--------------VQGVVYC 150
             Y PV +P PS     P+ +P PS       +PR                  V G+VYC
Sbjct: 250 VAYPPVMTPSPSPAAEPPIIAPFPSPPANPPLIPRRPAPPVVKPLPPLGKPPIVSGLVYC 309

Query: 151 KSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFH 210
           KSC   GV TLL A  L GA VKL C   K    V+ AT+D  G FR+  PK++T+    
Sbjct: 310 KSCNSYGVPTLLNASLLQGAVVKLICYGKK--TMVQWATTDNKGEFRI-MPKSLTTADVG 369

Query: 211 RCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVV--------LYNVGPLA 241
           +CKVYLV+SP+ NC   +  NGG+ G  LKP           VV        LY VGP  
Sbjct: 370 KCKVYLVKSPNPNCNVPTNFNGGKSGGLLKPLLPPKQPITPAVVPVQPPMSDLYGVGPFI 416

BLAST of Cmc12g0330981 vs. ExPASy TrEMBL
Match: A0A5A7URZ5 (Non-classical arabinogalactan protein 31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00870 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.2e-132
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH
Sbjct: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV
Sbjct: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240

Query: 241 PTCSG 246
           PTCSG
Sbjct: 241 PTCSG 245

BLAST of Cmc12g0330981 vs. ExPASy TrEMBL
Match: A0A1S3B5Q3 (non-classical arabinogalactan protein 31 OS=Cucumis melo OX=3656 GN=LOC103486459 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.2e-132
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH
Sbjct: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV
Sbjct: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240

Query: 241 PTCSG 246
           PTCSG
Sbjct: 241 PTCSG 245

BLAST of Cmc12g0330981 vs. ExPASy TrEMBL
Match: A0A0A0LXF0 (Structural constituent of cell wall OS=Cucumis sativus OX=3659 GN=Csa_1G074910 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 8.9e-115
Identity = 218/244 (89.34%), Postives = 226/244 (92.62%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           MASLTANASFL+LLL C  LNAFQAAAYSSTPAPAPTHHNAHHPVAAP PSFHH GHHHH
Sbjct: 1   MASLTANASFLLLLLCCTLLNAFQAAAYSSTPAPAPTHHNAHHPVAAPTPSFHHRGHHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HHSP+QSPTSHHHPHSPSPAPSPVYP  P AHYA    PVPSP HSPKPST +PRSFV+V
Sbjct: 61  HHSPTQSPTSHHHPHSPSPAPSPVYPLHPPAHYA----PVPSPAHSPKPSTNIPRSFVQV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSCKYP VDTLLGAKPLSGATVKLSCKNTKYAP VETATSD+NGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCKYPDVDTLLGAKPLSGATVKLSCKNTKYAPAVETATSDENGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS C+KASK+NGG DGAELKPARAFTDEEKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSKCEKASKMNGGVDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240

Query: 241 PTCS 245
           PTCS
Sbjct: 241 PTCS 240

BLAST of Cmc12g0330981 vs. ExPASy TrEMBL
Match: A0A6J1EHN2 (non-classical arabinogalactan protein 31-like OS=Cucurbita moschata OX=3662 GN=LOC111434378 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.6e-90
Identity = 186/244 (76.23%), Postives = 203/244 (83.20%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHH 60
           M S+   A  +++LLL C+LNAFQAAA   +P  AP   + H+PV   APS HHH HHHH
Sbjct: 1   MGSVATGA--VIVLLLGCALNAFQAAASHGSPTLAPRPDDRHYPVPVAAPSHHHH-HHHH 60

Query: 61  HHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEV 120
           HH PSQSP  HHH H  SPAPSPVY  PP AHYA    PVPSPV  PK STY+PRSFVEV
Sbjct: 61  HHPPSQSPIYHHHSH--SPAPSPVYAPPPPAHYA----PVPSPVQPPKRSTYIPRSFVEV 120

Query: 121 QGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNV 180
           QGVVYCKSC YPGVDTLLGAKPL+GATVKLSCKNTKYAPT+ETAT+DKNGYFRLAAPKNV
Sbjct: 121 QGVVYCKSCHYPGVDTLLGAKPLNGATVKLSCKNTKYAPTMETATTDKNGYFRLAAPKNV 180

Query: 181 TSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFE 240
           TSYAFHRCKVYLV+SPDS+C K SK+NGGEDGAELKPA+AFTD EKKPVVLYNVGPLAFE
Sbjct: 181 TSYAFHRCKVYLVKSPDSSCNKMSKMNGGEDGAELKPAKAFTDAEKKPVVLYNVGPLAFE 235

Query: 241 PTCS 245
           PTC+
Sbjct: 241 PTCA 235

BLAST of Cmc12g0330981 vs. ExPASy TrEMBL
Match: A0A6J1KM21 (non-classical arabinogalactan protein 31-like OS=Cucurbita maxima OX=3661 GN=LOC111495792 PE=4 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 3.3e-85
Identity = 174/234 (74.36%), Postives = 191/234 (81.62%), Query Frame = 0

Query: 11  LVLLLLCCSLNAFQAAAYSSTPAPAPTHHNAHHPVAAPAPSFHHHGHHHHHHSPSQSPTS 70
           +++LLL C+LNAFQAAA   +P PAP   + H+PV   APS     HHHHHH PSQSP  
Sbjct: 9   VIVLLLGCALNAFQAAANHGSPTPAPRPDDRHYPVPVAAPSH----HHHHHHPPSQSPIY 68

Query: 71  HHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSPKPSTYVPRSFVEVQGVVYCKSCK 130
           HHH H  SPAPSPVY  PP AHYA        PV  PK STY+PRSFVEVQGVVYCKSC 
Sbjct: 69  HHHAH--SPAPSPVYAPPPPAHYA--------PVQPPKRSTYIPRSFVEVQGVVYCKSCH 128

Query: 131 YPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFHRCKV 190
           YPGVDTLLGAKPL+GA VKLSCKNTKYAPTVETAT+DKNGYFRLAAPKNVTSYAFHRCKV
Sbjct: 129 YPGVDTLLGAKPLNGAAVKLSCKNTKYAPTVETATTDKNGYFRLAAPKNVTSYAFHRCKV 188

Query: 191 YLVESPDSNCKKASKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFEPTCS 245
           +LV+SPDS+C K SK+NGG DGAELKPA+AFTD EKKPVVLYNVGPLAFEP+C+
Sbjct: 189 HLVKSPDSSCSKMSKMNGGVDGAELKPAKAFTDAEKKPVVLYNVGPLAFEPSCA 228

BLAST of Cmc12g0330981 vs. TAIR 10
Match: AT1G28290.2 (arabinogalactan protein 31 )

HSP 1 Score: 142.5 bits (358), Expect = 4.5e-34
Identity = 117/285 (41.05%), Postives = 135/285 (47.37%), Query Frame = 0

Query: 29  SSTPAPAPTHHNAHHPVAAPAPSFHHHGH-HHHHHSPSQSPTSHH--------------- 88
           S  PAPAP HH  HH    P P  HHH H H H H P++SP                   
Sbjct: 35  SLAPAPAPYHHGHHH----PHPPHHHHPHPHPHPHPPAKSPVKPPVKAPVSPPAKPPVKP 94

Query: 89  ----------------------HPHSPSPAPSPVYP------------------FPPTAH 148
                                  P +  P   PVYP                  +PPT  
Sbjct: 95  PVYPPTKAPVKPPTKPPVKPPVSPPAKPPVKPPVYPPTKAPVKPPTKPPVKPPVYPPTKP 154

Query: 149 --YAPVPSPVPSPVHSP-----KPSTYVP---RSFVEVQGVVYCKSCKYPGVDTLLGAKP 208
             Y P  +PV  PV  P      P  Y P   RS V V+G VYCKSCKY   +TLLGAKP
Sbjct: 155 PVYPPTKAPVKPPVSPPTKPPVTPPVYPPKFNRSLVAVRGTVYCKSCKYAAFNTLLGAKP 214

Query: 209 LSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFHRCKVYLVESPDSNCKK 244
           + GATVKL CK+ K   T ET T+DKNGYF L APK VT++ F  C+VYLV+S D  C K
Sbjct: 215 IEGATVKLVCKSKKNI-TAET-TTDKNGYFLLLAPKTVTNFGFRGCRVYLVKSKDYKCSK 274

BLAST of Cmc12g0330981 vs. TAIR 10
Match: AT2G34700.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 139.4 bits (350), Expect = 3.8e-33
Identity = 75/147 (51.02%), Postives = 92/147 (62.59%), Query Frame = 0

Query: 102 SPVHSPKPSTYVPRSFVEVQGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTV 161
           SP+  P     + R  V V+G+VYCKSCKY GVDTLL A PL GATVKL+C NTK   T+
Sbjct: 30  SPMTPPSSPAKMSRRLVAVEGMVYCKSCKYSGVDTLLEASPLQGATVKLACNNTKRGVTM 89

Query: 162 ETATSDKNGYFRLAAPKNVTSYAFHRCKVYLVE----SPDSNCKKASKLNGGEDGAELKP 221
           ET T DKNGYF + APK +T+YAFH C+ +       +    C   SKLN G  GA LKP
Sbjct: 90  ETKT-DKNGYFFMLAPKKLTTYAFHTCRAWPTNPGPTTATMTCTVPSKLNNGITGAMLKP 149

Query: 222 ARAFTDEEKKPVVLYNVGPLAFEPTCS 245
           ++     E    VL++VGP AFEP C+
Sbjct: 150 SKTINIGE-HDYVLFSVGPFAFEPACA 174

BLAST of Cmc12g0330981 vs. TAIR 10
Match: AT1G28290.1 (arabinogalactan protein 31 )

HSP 1 Score: 136.3 bits (342), Expect = 3.2e-32
Identity = 109/230 (47.39%), Postives = 125/230 (54.35%), Query Frame = 0

Query: 28  YSSTPAPA--PTHHNAHHPVAAPAPSFHHHGHHHHHHSPSQSPTSH--HHPHSPSPAPSP 87
           Y  T AP   PT      PV  P              +P + PT      P SP PA  P
Sbjct: 145 YPPTKAPVKPPTKPPVKPPVYPPT------------KAPVKPPTKPPVKPPVSP-PAKPP 204

Query: 88  VYP--FPPTAHYAPVPSPVPSPVHSP-KPSTYVP---RSFVEVQGVVYCKSCKYPGVDTL 147
           V P  +PPT   APV  PV  P   P  P  Y P   RS V V+G VYCKSCKY   +TL
Sbjct: 205 VKPPVYPPTK--APVKPPVSPPTKPPVTPPVYPPKFNRSLVAVRGTVYCKSCKYAAFNTL 264

Query: 148 LGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFRLAAPKNVTSYAFHRCKVYLVESPD 207
           LGAKP+ GATVKL CK+ K   T ET T+DKNGYF L APK VT++ F  C+VYLV+S D
Sbjct: 265 LGAKPIEGATVKLVCKSKKNI-TAET-TTDKNGYFLLLAPKTVTNFGFRGCRVYLVKSKD 324

Query: 208 SNCKKASKLNGGEDGAELKPARAFTDE----EKKPVVLYNVGPLAFEPTC 244
             C K SKL GG+ GAELKP +          K    L+NVGP AF P+C
Sbjct: 325 YKCSKVSKLFGGDVGAELKPEKKLGKSTVVVNKLVYGLFNVGPFAFNPSC 357

BLAST of Cmc12g0330981 vs. TAIR 10
Match: AT2G33790.1 (arabinogalactan protein 30 )

HSP 1 Score: 109.0 bits (271), Expect = 5.5e-24
Identity = 91/254 (35.83%), Postives = 126/254 (49.61%), Query Frame = 0

Query: 1   MASLTANASFLVLLLLCCSLNAFQAAAY---SSTPAPAPTHHNAHHPVAAPAPSFHHHGH 60
           M  +  + S  +  LLC + + F        SS P  +   H    P+  P         
Sbjct: 1   MGIIGKSVSLTLFALLCFTSSVFTLGVNQPGSSDPFHSLPQHLPLPPIKLPT-------- 60

Query: 61  HHHHHSPSQSPTSHHHPHSPSPAPSPVYPFPPTAHYAPVPSPVPSPVHSP-KPSTYVP-- 120
                 P+++P      + P+ AP  +   PP    AP+  P   P+  P  P  Y P  
Sbjct: 61  ----LPPAKAPIK-LPAYPPAKAPIKLPTLPPAK--APIKLPTLPPIKPPVLPPVYPPKY 120

Query: 121 -RSFVEVQGVVYCKSCKYPGVDTLLGAKPLSGATVKLSCKNTKYAPTVETATSDKNGYFR 180
            ++ V V+GVVYCK+CKY GV+ + GAKP+  A V+L CKN K   ++    +DKNGYF 
Sbjct: 121 NKTLVAVRGVVYCKACKYAGVNNVQGAKPVKDAVVRLVCKNKK--NSISETKTDKNGYFM 180

Query: 181 LAAPKNVTSYAFHRCKVYLVESPDSNCKKASKLNGGEDGAELKPAR----AFTDEEKKPV 240
           L APK VT+Y    C+ +LV+SPD+ C K S L+ G  G+ LKP      + T       
Sbjct: 181 LLAPKTVTNYDIKGCRAFLVKSPDTKCSKVSSLHDGGKGSVLKPVLKPGFSSTIMRWFKY 237

Query: 241 VLYNVGPLAFEPTC 244
            +YNVGP AFEPTC
Sbjct: 241 SVYNVGPFAFEPTC 237

BLAST of Cmc12g0330981 vs. TAIR 10
Match: AT5G05500.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.3e-11
Identity = 48/158 (30.38%), Postives = 72/158 (45.57%), Query Frame = 0

Query: 90  TAHYAPVPSPVPSPVHSPKPSTYVPRSFVEVQGVVYCKSCKYPGVDTLLGAKPLSGATVK 149
           TA  A   +P P P  +  P+     + VE  G+VYC+SC   G  +L GA+ ++GA + 
Sbjct: 17  TAVLANAYTPPPPPPTTAYPAVKTVEAAVE--GMVYCQSCDKFGSWSLAGAEAIAGAKIS 76

Query: 150 LSCKNTKYAPT-VETATSDKNGYF-----RLAAPKNVTSYAFHRCKVYLVESPDSNCKKA 209
           + CKN +   +  +   +D  G+F           +   +  H C+  LV SP  +C   
Sbjct: 77  IICKNHRQQVSFYKVFRTDSYGHFYGELKGFKMTPHFLDHPLHSCRAKLVSSPREDCNLF 136

Query: 210 SKLNGGEDGAELKPARAFTDEEKKPVVLYNVGPLAFEP 242
           S +N   DGA L+             V+Y  GPLAF P
Sbjct: 137 SNINNALDGAPLRYEEKRLKWTNYEAVIYAAGPLAFRP 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008442660.12.6e-132100.00PREDICTED: non-classical arabinogalactan protein 31 [Cucumis melo] >KAA0056996.1... [more]
XP_004146606.21.8e-11489.34non-classical arabinogalactan protein 31 [Cucumis sativus] >KGN64686.1 hypotheti... [more]
XP_038876988.11.9e-11189.30non-classical arabinogalactan protein 31 [Benincasa hispida][more]
KAG7019613.12.4e-9076.64Non-classical arabinogalactan protein 31, partial [Cucurbita argyrosperma subsp.... [more]
XP_022927607.15.4e-9076.23non-classical arabinogalactan protein 31-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FZA24.5e-3147.39Non-classical arabinogalactan protein 31 OS=Arabidopsis thaliana OX=3702 GN=AGP3... [more]
P930137.7e-2335.83Non-classical arabinogalactan protein 30 OS=Arabidopsis thaliana OX=3702 GN=AGP3... [more]
Q032112.0e-1836.36Pistil-specific extensin-like protein OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7URZ51.2e-132100.00Non-classical arabinogalactan protein 31 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3B5Q31.2e-132100.00non-classical arabinogalactan protein 31 OS=Cucumis melo OX=3656 GN=LOC103486459... [more]
A0A0A0LXF08.9e-11589.34Structural constituent of cell wall OS=Cucumis sativus OX=3659 GN=Csa_1G074910 P... [more]
A0A6J1EHN22.6e-9076.23non-classical arabinogalactan protein 31-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KM213.3e-8574.36non-classical arabinogalactan protein 31-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT1G28290.24.5e-3441.05arabinogalactan protein 31 [more]
AT2G34700.13.8e-3351.02Pollen Ole e 1 allergen and extensin family protein [more]
AT1G28290.13.2e-3247.39arabinogalactan protein 31 [more]
AT2G33790.15.5e-2435.83arabinogalactan protein 30 [more]
AT5G05500.15.3e-1130.38Pollen Ole e 1 allergen and extensin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003882Pistil-specific extensin-like proteinPRINTSPR01218PSTLEXTENSINcoord: 129..153
score: 56.0
coord: 230..245
score: 33.33
coord: 64..87
score: 31.25
NoneNo IPR availablePFAMPF01190Pollen_Ole_e_1coord: 120..212
e-value: 1.1E-25
score: 89.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..84
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..68
NoneNo IPR availablePANTHERPTHR33470OS01G0164075 PROTEINcoord: 9..243
NoneNo IPR availablePANTHERPTHR33470:SF22POLLEN OLE E 1 ALLERGEN AND EXTENSIN FAMILY PROTEINcoord: 9..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc12g0330981.1Cmc12g0330981.1mRNA