Cmc08g0219261 (gene) Melon (Charmono) v1.1

Overview
NameCmc08g0219261
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
LocationCMiso1.1chr08: 7394097 .. 7398168 (-)
RNA-Seq ExpressionCmc08g0219261
SyntenyCmc08g0219261
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTACATTTCTCAGAAATCAAAACAACAAAAATATAAACATTTCTACTTATAGAAATATGAATAGTTTTTTTTTTTTTTCCTCTTTTTCTTCAACGGATAGTGCTGATACGATGGCTTCTTATCCGATTGGCCGTGGAGTCTGAAATTAGATAATCATTTCTTTAATGGAATTTAGGGAAATATGTCAGAATGCAGATCTCTCCTTTTCTTCATTTATCTTACGAACCCCAACAAATTTATGCTCTATTTTCCGAAGTAATTCATCAATCTAGAGCACTCTCCGTCCAGAATGGCTTTTCAAGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGCTTTCTTCTTGGGTCCGCTGGAAATGGGTTTCTTCAATTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAGGTGGGGAAACAGCTGGATTGTGTCAACGATGATCGTGACAATCAGGTAGAATACACTCTGATATGGTCAACTTTGATTATTTTCTTCCAATTTCATATTTCATATTCTTTGTTTTTATAATCCCAAATCGTTTTTAGATAAGATGCCTCTTAGTTTTTTAATCGTTATGTTGACGAGTGAGATTGTGTTATTGTGATTACTGCATCGCTATAATTATTTTTCTCAAAATGGGAACTGACTGGTTGACTTTGAACTGTATTTTTTTATTTTTCATTCGGCTTGTCAACTCCCTCCTCCTTAGTAGATGTTAGACTTTGAGAGGAATTTCTATTTGAACTGAGAAAGATATTAGTTAAGACTTTGAGGAGAATTTCTGTGTTTTAGAGTGCGCCACCCCCCGAGTTACAAGTCCATATGGTTATTCTTCCACTTATGTAGTGTGGAAGGTGGAAAATGTATGCTAAAAAAGAGCTGCAAAAGAGATTACTTTGTTCATCGAGATAATGTATTTTGAAAAATATGCATTTCAAAAAGAGGAGCCAAGCAACAATAGCGAAAAGGATCTCCAATCCAGTAAAATAAGATGTAGAAGGTTAATCCCGAAAAGACCTTGTCACCGATGTGCAAAGAGAAAGATAGAATCTATCAAGGTCAAACATTGCTCTGCTATCTCTCCTACCATGCTAAAATAGAGACATCCTCTCAAGCTTATCTTAAATCTCTCTGTTCTTCTTACCAATTGTGAACAAGTTTATGATATTCAACGATATGCCATTATGTGCATGTTCCTGATGTCATCTGTTGAAAGTGAAATTTGCATTTATTGAACAAGGTTTTGGAGTTTCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCAGCCTAGTATGTACAGTAATTTTACACAGGTAATTGTATGCTGAAAATGCCCCAACTCCAAGTGCAACTATAGATGTTTATGAAATTGATAGTTTTTTTCATTTAGGTGAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACTATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCTCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCAGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGGTTACTTCATATCTCCTCCTCGTCATCCTCCTTAATAATAGAAGTACATTCTCATCCTCGCATGGAAAGAACACTATTTGCGTACACTAACATCTTATCTAATTAGTTCACATTTGTTAGATTGGTAATTCTTAGTGTAATTCTCCCATGTCATACAGTACAGGGAGGCAGGAGTACTTATATGGCGTGGCTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGCGACACAATTACTTCACTGTAGCTTCAACAATTGCGTGAGATTTGAGCTGTCTAAACTTTTTAACCAGATCATCCAAGACTTCAAATCTTATTACTCGTATTTTACCTTAATTTCCAGTTCTCAAATTCCTCATGCTGTTGGCGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCGCTTATTTTGGCGATGGAGGAACAAGTGAGGTACTGAATACTATGAAAAAATCGTTAGAATGTACAGAACACTGTGAAAAACTCTTATTTGGAATGCTTGTTGATATTTTCTACATCGGTGTCTGTAGTTCTTCATATTTGATGCTTTGTGCTTCTTCTCCTTTTTCATTTTCTTATCATTCTTTGGAATTTACATTACTTGATCATTAGTCTCTTTTCATTTCATCGATGAAAATTTTTTGTTAACCTAATCTCCGTAGATTTTTGTGGAGATAAGCAGTTCAAATAGAAAGGCATTCTACTAAATTTGGTTGATTCTATGGAGTAATATTGTGTACTCTGGCTAGTTGCAAGGTTGATTTTTCTGTTGTTTCTTTTGTCATGTTACATTAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTGTTTCAGACCAGTTTCGAAGTATCTCATTCTTCTCGGTATTTCCAGTTGTGAAAAATGTATTATGAAATCTAGATTCTGAAGTGTTCAATTATTGTGCAGGTGATGGGGTGGTTGTTAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCTATTCAAGAACAGAGGCCAATTTTAATTGAGGTAACAAGTGCACACAAACTCTCTCTTAATATGGATACCTTTGATGATCTGGTGTCTTTTGTTTTCATTGACAGGCTCTCACATATAGAGCTGGACACCACACGACATCGGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATAAGGCTGAAACAGAGCTTAGAAGTAGCCTAAGAAAACAGGTATATTTTGTGATTAGAATAAAGTCTTTTCAACGCCGCACATACACAGCTACGTGCACACGCGTGCACACACGAGAGACCACTTCAATCTAAGAAAAAAAAAATGAAAAATAAGAAACAAAAAGGAACAATAAACAAGCAAAAGGACTTGACCAGAAAAGGTTACAAAAACACCATCCAATGTCAATGGTGGTCAGCGAGGTTAAACACCATCCGATGTCAATGGTGAAATTGGAGTTGAGTTGGGAAGAAACATAGTAAATGAGATAAACAAGAAAAGGATATATTCAACTCAAAATTATCATTTTCATTTAAGCTTCTTGAAGAAGAAAGACCTTTTTTTCTTTTTTTTTTTTTTTTTTCTTTTTTTGCCTTAAAACCATTTTTTCTTGAGACACTAAAAACATATTTTTGAGAGTCGTTCTTCTTTAAGTTTTTAATACTCAATTAAAATTTTGAAAATACTTTCACCAAGTAGATTTAAAGAAAAAGAAACAGTGAGTAAACAAAGTGTCTATCATCTACATGACTTATTTTGAAAATACTCTCGTCTCGAACAAATCCCTGTCTATCATGTACGTTTTTAGTTCACTGTGTTTTTTGGTTCCAACAGCTATTAGAGGAGATCCAGTTGGCAGAGAGATATGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTACCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAATTTTCCTCTGTAGTTTGTCATTGGGTTTTGAAACACTCTGCCTTATAGAAATATGAGAATGGTGATGACATTGTTTATGAATAAAGGCCATAAGGCATATTGATCTAATAATG

mRNA sequence

CCTACATTTCTCAGAAATCAAAACAACAAAAATATAAACATTTCTACTTATAGAAATATGAATAGTTTTTTTTTTTTTTCCTCTTTTTCTTCAACGGATAGTGCTGATACGATGGCTTCTTATCCGATTGGCCGTGGAGTCTGAAATTAGATAATCATTTCTTTAATGGAATTTAGGGAAATATGTCAGAATGCAGATCTCTCCTTTTCTTCATTTATCTTACGAACCCCAACAAATTTATGCTCTATTTTCCGAAGTAATTCATCAATCTAGAGCACTCTCCGTCCAGAATGGCTTTTCAAGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGCTTTCTTCTTGGGTCCGCTGGAAATGGGTTTCTTCAATTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAGGTGGGGAAACAGCTGGATTGTGTCAACGATGATCGTGACAATCAGGTTTTGGAGTTTCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCAGCCTAGTATGTACAGTAATTTTACACAGGTGAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACTATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCTCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCAGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGTACAGGGAGGCAGGAGTACTTATATGGCGTGGCTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGCGACACAATTACTTCACTGTAGCTTCAACAATTGCTTCTCAAATTCCTCATGCTGTTGGCGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCGCTTATTTTGGCGATGGAGGAACAAGTGAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTGTTTCAGACCAGTTTCGAAGTGATGGGGTGGTTGTTAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCTATTCAAGAACAGAGGCCAATTTTAATTGAGGCTCTCACATATAGAGCTGGACACCACACGACATCGGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATAAGGCTGAAACAGAGCTTAGAAGTAGCCTAAGAAAACAGCTATTAGAGGAGATCCAGTTGGCAGAGAGATATGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTACCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAATTTTCCTCTGTAGTTTGTCATTGGGTTTTGAAACACTCTGCCTTATAGAAATATGAGAATGGTGATGACATTGTTTATGAATAAAGGCCATAAGGCATATTGATCTAATAATG

Coding sequence (CDS)

ATGGCTTTTCAAGTGAGAAAAGCAACAACGAAGATCATTCCCCATTTTAGAAACGGGATGGCTTTCTTCTTGGGTCCGCTGGAAATGGGTTTCTTCAATTCTTATTCAACCCATCTTCGTAATACTTCATTTTCTGCTCATAAGATCGGAACCAGAATTTTGGGTTCTGAAAATATGCCTACCCATTTGTTTTCTCGTCGTTTTGAATCGATTAAGGTGGGGAAACAGCTGGATTGTGTCAACGATGATCGTGACAATCAGGTTTTGGAGTTTCCAGGAGGAAAGGTAAAATTTACTTCTCAACTGAGTTTCATCCCTGATCGCGAGGAGGAGCGGATACCTTGCTTTCGGGTTCTTGATGACAACGGCCAGCCTAGTATGTACAGTAATTTTACACAGGTGAGCAAGGAGGTCGCTATGAAGATATACCATGACATGGTTACCCTCCAAACAATGGACACTATCTTCTACGAAGCGCAAAGGCAAGGAAGAATTTCCTTTTATGTGACCTCTCTTGGAGAAGAAGCAATCAACGTTGCTTCTGCAGCAGCACTGTCATTGGATGACCTCATTGTTCCACAGTACAGGGAGGCAGGAGTACTTATATGGCGTGGCTTCACTCTGAAAGAATTTGCAAGCCAGTGCTTTGGAAACAAATATGATTATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCTAAGCGACACAATTACTTCACTGTAGCTTCAACAATTGCTTCTCAAATTCCTCATGCTGTTGGCGCTGCATATTCAATGAAGATGGATAAGAAAGATGCTTGTGCAGTCGCTTATTTTGGCGATGGAGGAACAAGTGAGGGAGATTTCCACGCAGCTTTAAATTTTGCCGCAGTTTTGGAGGCACCTGTTATCTTTATTTGCCGGAACAATGGATGGGCGATCAGTACTCCTGTTTCAGACCAGTTTCGAAGTGATGGGGTGGTTGTTAAAGGCCAGGCTTATGGAGTTCGCAGTATCCGTGTAGATGGCAATGATGCACTTGCTGTATATAATGCAGTTCGAGCTGCTCGAGAAATGGCTATTCAAGAACAGAGGCCAATTTTAATTGAGGCTCTCACATATAGAGCTGGACACCACACGACATCGGATGATTCGACCAGGTATCGTCCAGCTGAGGAAATGGAATGGTGGAGAGTTGCAATGGACCCTGTCTCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATAAGGCTGAAACAGAGCTTAGAAGTAGCCTAAGAAAACAGCTATTAGAGGAGATCCAGTTGGCAGAGAGATATGAGAAGCCCCCTGTGGCAGATGCTTTCACAGATGTGTATGATGTACCTCCTTCCAACCTCCAAGAGCAAGAGAAGTGGTTAAGAAAAACAATCAAAACACACCCACAAGATTATCCATCTAATTTTCCTCTGTAG

Protein sequence

MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMPTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFPL
Homology
BLAST of Cmc08g0219261 vs. NCBI nr
Match: XP_008441060.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumis melo] >KAA0025521.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa] >TYK25677.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa])

HSP 1 Score: 981.9 bits (2537), Expect = 2.0e-282
Identity = 480/481 (99.79%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSN+AETELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Cmc08g0219261 vs. NCBI nr
Match: XP_004148662.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumis sativus] >KGN48345.1 hypothetical protein Csa_003053 [Cucumis sativus])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 469/481 (97.51%), Postives = 475/481 (98.75%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGM FFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQL+CVNDDRD+QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPA+EMEWWRVAMDPVSRFRKWIESNGWWSN+AE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           S LRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Cmc08g0219261 vs. NCBI nr
Match: XP_038883947.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 908.3 bits (2346), Expect = 2.8e-260
Identity = 444/481 (92.31%), Postives = 460/481 (95.63%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           M F VRKATTKIIPHFRNGM+ FLG LEMGFFNSYSTHLRNTSF+A+KIGT  L SE+MP
Sbjct: 1   MVFHVRKATTKIIPHFRNGMS-FLGSLEMGFFNSYSTHLRNTSFAAYKIGTGALDSEDMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THL +RRFESIK GKQLD VNDD DNQVLEFPGGKVKFTSQLSFIP REEERIPCFRVLD
Sbjct: 61  THLSARRFESIKAGKQLDSVNDDCDNQVLEFPGGKVKFTSQLSFIPYREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSMYSNFTQVSKEVAMK+Y DMVTLQTMDTIFYEAQRQGRISFY+TSLGEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKMYSDMVTLQTMDTIFYEAQRQGRISFYLTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALS DDLIVPQYREAGVLIWRGFTLKEFASQCF NKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSFDDLIVPQYREAGVLIWRGFTLKEFASQCFSNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDK+DACAV YFGDGGTSEGDFH ALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKRDACAVTYFGDGGTSEGDFHGALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKG+AYGVRSIR+DGNDALAVYNAVRAAR+MAIQE +P
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRIDGNDALAVYNAVRAARKMAIQEHKP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVA DPVSRFRKWIESNGWW+N+AE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAKDPVSRFRKWIESNGWWNNEAEAELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 480

BLAST of Cmc08g0219261 vs. NCBI nr
Match: XP_022132554.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charantia])

HSP 1 Score: 855.5 bits (2209), Expect = 2.2e-244
Identity = 419/482 (86.93%), Postives = 451/482 (93.57%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNS-YSTHLRNTSFSAHKIGTRILGSENM 60
           MAFQ RKA TKIIPHF N M  FLG +EMGF +S Y+T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQARKA-TKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 60

Query: 61  PTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120
           PTHL +RRF+SIKV K+LD VNDDRDNQVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 120

Query: 121 DDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180
           DDNGQ S++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 180

Query: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 240

Query: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIF 300
           FTVASTIASQIPHAVGAAYSMKM+KKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 301 ICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360
           ICRNNGWAISTP+SDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETEL 420
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSN+AE+EL
Sbjct: 361 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 420

Query: 421 RSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 480

Query: 481 PL 482
           PL
Sbjct: 481 PL 481

BLAST of Cmc08g0219261 vs. NCBI nr
Match: XP_023543630.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 851.3 bits (2198), Expect = 4.1e-243
Identity = 417/481 (86.69%), Postives = 442/481 (91.89%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAF VRKA TKIIPHFRNGM  FLG             LR T F+ HKIG ++LG ENM 
Sbjct: 1   MAFHVRKA-TKIIPHFRNGMR-FLG------------SLRETPFAVHKIGNKVLGFENML 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
            HL + RF+S KV KQ+D VNDDRDNQVL FPGGKVKFT QLSF+P+REEER+PCFRVLD
Sbjct: 61  NHLSAHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSM+SNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAIN+A
Sbjct: 121 DNGQPSMFSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAV YFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN VRAAR+MAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYR GHHTTSDDSTRYRP EEMEWWRVA DPVSRFRKWIESNGWWS++AE ELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNL+EQE+WLRKTIKTHPQDYPS+FP
Sbjct: 421 SSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFP 467

Query: 481 L 482
           L
Sbjct: 481 L 467

BLAST of Cmc08g0219261 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 1.4e-177
Identity = 292/397 (73.55%), Postives = 351/397 (88.41%), Query Frame = 0

Query: 85  DNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYH 144
           ++QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNGQ    S F QVS+EVA+KIY 
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 145 DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWR 204
           DMVTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 205 GFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDK 264
           GFTL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDK
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 265 KDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVK 324
           KDACAV YFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 325 GQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRP 384
           G+AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 385 AEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVAD 444
           A E+EWW  A +P+SRFR WIESNGWWS+K E++LRS ++K++LE +++AE+ EKP + +
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 445 AFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFPL 482
            F+DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of Cmc08g0219261 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 8.5e-167
Identity = 275/418 (65.79%), Postives = 346/418 (82.78%), Query Frame = 0

Query: 65  SRRFESIKVGKQLDCV---NDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDD 124
           S R ES  V  Q D +    D+ D Q L+FPGGKV +TS++ FIP+    RIPC+RVLD+
Sbjct: 53  SLRHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDE 112

Query: 125 NGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVAS 184
           +G+    S+F  VS+++A+++Y  M TLQ MD IFYEAQRQGRISFY+TS+GEEAIN+AS
Sbjct: 113 DGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIAS 172

Query: 185 AAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFT 244
           AAALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R NYFT
Sbjct: 173 AAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFT 232

Query: 245 VASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFIC 304
           ++S IA+Q+P A G  YS+KMDKK+AC V + GDGGTSEGDFHA LNFAAV+EAPV+FIC
Sbjct: 233 ISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFIC 292

Query: 305 RNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPI 364
           RNNGWAIST +S+QFRSDG+VVKGQAYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+
Sbjct: 293 RNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPV 352

Query: 365 LIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRS 424
           LIE +TYR GHH+TSDDST+YR A+E+++W+++ +PV+RFRKW+E NGWWS + E++LRS
Sbjct: 353 LIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRS 412

Query: 425 SLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480
           + RKQLL+ IQ AE++EK P+ + F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 413 NARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

BLAST of Cmc08g0219261 vs. ExPASy Swiss-Prot
Match: Q54M22 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=bkdA PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.2e-109
Identity = 184/367 (50.14%), Postives = 255/367 (69.48%), Query Frame = 0

Query: 113 IPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSL 172
           IPC+ ++D  G  S        SKE  +K+Y  M+TL  MD+I Y+ QRQGRISFY+TS 
Sbjct: 64  IPCYTIMDQEGVVSKPDQDPNFSKEEVIKMYTTMLTLNVMDSILYDVQRQGRISFYMTSF 123

Query: 173 GEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 232
           GEEAI++ SAAAL + D I  QYRE GV +WRGFT+ +  +QC  N++D  KGRQMP+H+
Sbjct: 124 GEEAIHIGSAAALEMSDTIFAQYRETGVFMWRGFTINDIINQCCTNEHDLGKGRQMPMHF 183

Query: 233 GSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAV 292
           GS++ N  T++S + +Q+P AVG++Y+ K+  +  C + YFG+G  SEGDFHAA+NFAA 
Sbjct: 184 GSRKINLQTISSPLTTQLPQAVGSSYAQKLAGEKNCTIVYFGEGAASEGDFHAAMNFAAA 243

Query: 293 LEAPVIFICRNNGWAISTPVSDQFRSDGVVVKG-QAYGVRSIRVDGNDALAVYNAVRAAR 352
           L  P IF CRNN WAISTP  +Q++ DG+  +G   YG+++IRVDGND  AVYN  + AR
Sbjct: 244 LSTPTIFFCRNNKWAISTPSKEQYKGDGIAGRGPNGYGMKTIRVDGNDIWAVYNVTKLAR 303

Query: 353 EMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWW 412
           ++A++EQ P+LIEA+TYR GHH+TSDDS+RYR  EE+  W+   +P+SR R ++   GWW
Sbjct: 304 KIAVEEQVPVLIEAMTYRVGHHSTSDDSSRYRTVEEINAWKEGKNPISRLRNYMNHKGWW 363

Query: 413 SNKAETELRSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTH 472
           S+  E E  ++ R  + E +  AE+  KP + + FTDVYD P  NL EQ+K L + +K +
Sbjct: 364 SDAQEKETIANARTTVRESLVNAEKQYKPSINEIFTDVYDKPTPNLIEQQKELIEHLKLY 423

Query: 473 PQDYPSN 479
           P +YP N
Sbjct: 424 PDEYPLN 430

BLAST of Cmc08g0219261 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.7e-109
Identity = 192/387 (49.61%), Postives = 255/387 (65.89%), Query Frame = 0

Query: 90  EFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYHDMVTL 149
           +FPG   +F  +L FI       IP +RV+D  GQ    S    + KE  +K+Y  M  L
Sbjct: 53  QFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLL 112

Query: 150 QTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLK 209
            TMD I YE+QRQGRISFY+T+ GEE  +V SAAAL   DL+  QYREAGVL++R + L+
Sbjct: 113 NTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLE 172

Query: 210 EFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACA 269
            F +QC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    +   
Sbjct: 173 LFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVV 232

Query: 270 VAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVKGQAYG 329
           + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G  YG
Sbjct: 233 ICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYG 292

Query: 330 VRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEME 389
           + SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR GHH+TSDDS+ YR  +E+ 
Sbjct: 293 IMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVN 352

Query: 390 WWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVADAFTDV 449
           +W     PVSR R ++ S GWW  + E   R   RK+++E  + AER  KP     F+DV
Sbjct: 353 YWDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQAERKPKPNPNLLFSDV 412

Query: 450 YDVPPSNLQEQEKWLRKTIKTHPQDYP 477
           Y   P+ L++Q++ L + ++T+ + YP
Sbjct: 413 YQEMPAQLRKQQESLARHLQTYGEHYP 439

BLAST of Cmc08g0219261 vs. ExPASy Swiss-Prot
Match: P50136 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.1e-108
Identity = 189/387 (48.84%), Postives = 256/387 (66.15%), Query Frame = 0

Query: 90  EFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYHDMVTL 149
           +FPG   +F  +L FI       IP +RV+D  GQ    S    + +E  +K Y  M  L
Sbjct: 50  QFPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRSMTLL 109

Query: 150 QTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLK 209
            TMD I YE+QR+GRISFY+T+ GEE  +V SAAAL   DL+  QYREAGVL++R + L+
Sbjct: 110 NTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLE 169

Query: 210 EFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACA 269
            F SQC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    +   
Sbjct: 170 LFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRIV 229

Query: 270 VAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVKGQAYG 329
           + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G  YG
Sbjct: 230 ICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYG 289

Query: 330 VRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEME 389
           ++SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR GHH+TSDDS+ YR  +E+ 
Sbjct: 290 IKSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVN 349

Query: 390 WWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVADAFTDV 449
           +W     P+SR R+++ + GWW  + E   R   RK+++E  + AER  KP  +  F+DV
Sbjct: 350 YWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDV 409

Query: 450 YDVPPSNLQEQEKWLRKTIKTHPQDYP 477
           Y   P+ L+ Q++ L + ++T+ + YP
Sbjct: 410 YQEMPAQLRRQQESLARHLQTYGEHYP 436

BLAST of Cmc08g0219261 vs. ExPASy TrEMBL
Match: A0A5A7SMQ8 (2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00070 PE=4 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 9.8e-283
Identity = 480/481 (99.79%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSN+AETELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Cmc08g0219261 vs. ExPASy TrEMBL
Match: A0A1S3B380 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485287 PE=4 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 9.8e-283
Identity = 480/481 (99.79%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSN+AETELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Cmc08g0219261 vs. ExPASy TrEMBL
Match: A0A0A0KF52 (E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.1e-277
Identity = 469/481 (97.51%), Postives = 475/481 (98.75%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAFQVRKATTKIIPHFRNGM FFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
           THLFSRRFESIKVGKQL+CVNDDRD+QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD
Sbjct: 61  THLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNG+P MYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA
Sbjct: 121 DNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKMDKKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYRAGHHTTSDDSTRYRPA+EMEWWRVAMDPVSRFRKWIESNGWWSN+AE ELR
Sbjct: 361 ILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           S LRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP
Sbjct: 421 SRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Cmc08g0219261 vs. ExPASy TrEMBL
Match: A0A6J1BTD3 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005384 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 419/482 (86.93%), Postives = 451/482 (93.57%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNS-YSTHLRNTSFSAHKIGTRILGSENM 60
           MAFQ RKA TKIIPHF N M  FLG +EMGF +S Y+T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQARKA-TKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 60

Query: 61  PTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120
           PTHL +RRF+SIKV K+LD VNDDRDNQVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 120

Query: 121 DDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180
           DDNGQ S++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 180

Query: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 240

Query: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIF 300
           FTVASTIASQIPHAVGAAYSMKM+KKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 301 ICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360
           ICRNNGWAISTP+SDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETEL 420
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSN+AE+EL
Sbjct: 361 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 420

Query: 421 RSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 480

Query: 481 PL 482
           PL
Sbjct: 481 PL 481

BLAST of Cmc08g0219261 vs. ExPASy TrEMBL
Match: A0A6J1GE31 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453112 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.6e-243
Identity = 417/481 (86.69%), Postives = 441/481 (91.68%), Query Frame = 0

Query: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILGSENMP 60
           MAF VRKA TKIIPHFRNGM  FLG             LR T F+ HKIG R+LG ENM 
Sbjct: 1   MAFHVRKA-TKIIPHFRNGMR-FLG------------SLRETPFAVHKIGNRVLGFENML 60

Query: 61  THLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLD 120
            HL + RF+S KV KQ+D VNDDRDNQVL FPGGKVKFT QLSF+P+REEER+PCFRVLD
Sbjct: 61  NHLSTHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 121 DNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVA 180
           DNGQPSMYSNFTQVSKE+AMK+Y+DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAIN+A
Sbjct: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 241 TVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300
           TVASTIASQIPHAVGAAYSMKM+K+DACAV YFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 301 CRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360
           CRNNGWAISTP+SDQFRSDGVVVKG+AYGVRSIRVDGNDALAVYN VRAAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRP 360

Query: 361 ILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELR 420
           ILIEALTYR GHHTTSDDSTRYRP EEMEWWRVA DPVSRFRKWIESNGWWS++AE ELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 421 SSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFP 480
           SSLRKQLLEEIQLAER EKPPVADAFTDVYDVPPSNL+EQE+WLRKTIKTHPQDYPS+F 
Sbjct: 421 SSLRKQLLEEIQLAERTEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQ 467

Query: 481 L 482
           L
Sbjct: 481 L 467

BLAST of Cmc08g0219261 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 624.0 bits (1608), Expect = 9.9e-179
Identity = 292/397 (73.55%), Postives = 351/397 (88.41%), Query Frame = 0

Query: 85  DNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYH 144
           ++QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNGQ    S F QVS+EVA+KIY 
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 145 DMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWR 204
           DMVTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 205 GFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDK 264
           GFTL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDK
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 265 KDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVK 324
           KDACAV YFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 325 GQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRP 384
           G+AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 385 AEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVAD 444
           A E+EWW  A +P+SRFR WIESNGWWS+K E++LRS ++K++LE +++AE+ EKP + +
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 445 AFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFPL 482
            F+DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

BLAST of Cmc08g0219261 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 623.2 bits (1606), Expect = 1.7e-178
Identity = 292/395 (73.92%), Postives = 349/395 (88.35%), Query Frame = 0

Query: 87  QVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDDNGQPSMYSNFTQVSKEVAMKIYHDM 146
           QV++FPGGKV FT ++ FI + ++ER+PC+RVLDDNGQ    S F QVS+EVA+KIY DM
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66

Query: 147 VTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVASAAALSLDDLIVPQYREAGVLIWRGF 206
           VTLQ MD IFYEAQRQGR+SFY T++GEEAIN+ASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 67  VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 126

Query: 207 TLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKD 266
           TL+EFA+QCFGNK DY KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KMDKKD
Sbjct: 127 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 186

Query: 267 ACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVSDQFRSDGVVVKGQ 326
           ACAV YFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGVVVKG+
Sbjct: 187 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 246

Query: 327 AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAE 386
           AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYR GHH+TSDDSTRYR A 
Sbjct: 247 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 306

Query: 387 EMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQLAERYEKPPVADAF 446
           E+EWW  A +P+SRFR WIESNGWWS+K E++LRS ++K++LE +++AE+ EKP + + F
Sbjct: 307 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 366

Query: 447 TDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNFPL 482
           +DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 367 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

BLAST of Cmc08g0219261 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 588.2 bits (1515), Expect = 6.0e-168
Identity = 275/418 (65.79%), Postives = 346/418 (82.78%), Query Frame = 0

Query: 65  SRRFESIKVGKQLDCV---NDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVLDD 124
           S R ES  V  Q D +    D+ D Q L+FPGGKV +TS++ FIP+    RIPC+RVLD+
Sbjct: 53  SLRHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDE 112

Query: 125 NGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINVAS 184
           +G+    S+F  VS+++A+++Y  M TLQ MD IFYEAQRQGRISFY+TS+GEEAIN+AS
Sbjct: 113 DGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIAS 172

Query: 185 AAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYFT 244
           AAALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK DY KGRQMP+HYGS R NYFT
Sbjct: 173 AAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFT 232

Query: 245 VASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFIC 304
           ++S IA+Q+P A G  YS+KMDKK+AC V + GDGGTSEGDFHA LNFAAV+EAPV+FIC
Sbjct: 233 ISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFIC 292

Query: 305 RNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPI 364
           RNNGWAIST +S+QFRSDG+VVKGQAYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+
Sbjct: 293 RNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPV 352

Query: 365 LIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRS 424
           LIE +TYR GHH+TSDDST+YR A+E+++W+++ +PV+RFRKW+E NGWWS + E++LRS
Sbjct: 353 LIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRS 412

Query: 425 SLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480
           + RKQLL+ IQ AE++EK P+ + F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 413 NARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

BLAST of Cmc08g0219261 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-84
Identity = 143/222 (64.41%), Postives = 186/222 (83.78%), Query Frame = 0

Query: 254 VGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPVS 313
           +G + +    +K+ACAV + GDGGTSEGDFHA LNFAAV+EAPV+FICRNNGWAIST +S
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 314 DQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRAGHH 373
           +QFRSDG+VVKGQAYG+RSIRVDGNDALAVY+AV +AREMA+ EQRP+LIE + YR GHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 374 TTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNKAETELRSSLRKQLLEEIQL 433
           +TSDDST+YR A+E+++W+++ + V+RFRK +E NGWWS + E++LRS+ RKQLL+ IQ 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 434 AERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDY 476
           AE++EK P+ + F DVYDV P NL+E+E  L++ I+  PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

BLAST of Cmc08g0219261 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 149.1 bits (375), Expect = 9.4e-36
Identity = 83/278 (29.86%), Postives = 148/278 (53.24%), Query Frame = 0

Query: 173 GEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 232
           G+EA+ +   AA++  D I+  YR+  + + RG +L E  S+  G +    KG+   +H+
Sbjct: 94  GQEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHF 153

Query: 233 GSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAV 292
             K  +++     + +Q+P   G A++ K +K++A   A +GDG  ++G    ALN +A+
Sbjct: 154 YKKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISAL 213

Query: 293 LEAPVIFICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAARE 352
            + P I +C NN + + T      +S     +G    V  ++VDG DA AV  A + A++
Sbjct: 214 WDLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQ 273

Query: 353 MAIQEQRPILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWS 412
            A+ E+ PI++E  TYR   H+ SD  + YR  +E+   R   DP+ R +K + S+   +
Sbjct: 274 HAL-EKGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLAT 333

Query: 413 NKAETELRSSLRKQLLEEIQLAERYEKPPVADAFTDVY 451
            K   ++   +RK++ + I  A+    P  ++ FT+VY
Sbjct: 334 EKELKDMEKEIRKEVDDAIAKAKDCPMPEPSELFTNVY 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008441060.12.0e-28299.79PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumi... [more]
XP_004148662.11.3e-27697.512-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumi... [more]
XP_038883947.12.8e-26092.312-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida... [more]
XP_022132554.12.2e-24486.932-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charant... [more]
XP_023543630.14.1e-24386.692-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
Q84JL21.4e-17773.552-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha... [more]
Q9LPL58.5e-16765.792-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha... [more]
Q54M221.2e-10950.142-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium dis... [more]
A5A6H94.7e-10949.612-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O... [more]
P501361.1e-10848.842-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=1... [more]
Match NameE-valueIdentityDescription
A0A5A7SMQ89.8e-28399.792-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3B3809.8e-28399.792-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX... [more]
A0A0A0KF526.1e-27797.51E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 ... [more]
A0A6J1BTD31.1e-24486.932-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica chara... [more]
A0A6J1GE317.6e-24386.692-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT5G09300.19.9e-17973.55Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.21.7e-17873.92Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G21400.16.0e-16865.79Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G34780.11.1e-8464.41Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G59900.19.4e-3629.86pyruvate dehydrogenase complex E1 alpha subunit [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 146..441
e-value: 6.1E-94
score: 314.3
NoneNo IPR availableGENE3D3.40.50.970coord: 84..479
e-value: 2.0E-145
score: 486.7
NoneNo IPR availablePANTHERPTHR433802-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIALcoord: 63..481
NoneNo IPR availablePANTHERPTHR43380:SF112-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIALcoord: 63..481
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 142..434
e-value: 1.71057E-145
score: 416.126
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 116..477

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc08g0219261.1Cmc08g0219261.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009083 branched-chain amino acid catabolic process
cellular_component GO:0005947 mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor