Cmc07g0198351 (gene) Melon (Charmono) v1.1

Overview
NameCmc07g0198351
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSubtilisin-like protease SBT2.5
LocationCMiso1.1chr07: 21552955 .. 21554176 (-)
RNA-Seq ExpressionCmc07g0198351
SyntenyCmc07g0198351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGCTCTCCTCTTCAGTAACCAAAATTAAACTTTATATTTTTAAACAAATAAATCTAACCGACCCTAATTAAATTATACTTACATCATATAGATATATATATGTGTGTGTGTAGTAGTTTATGCATTATTAAAGAGACTTCAATATTTTCCCTAAGGAAGAAAACTACAAAATGCAGGAGAACAGATACAACGGAGCACATGAACTCAGTGGCGGTGGTGGTGGGGGCAGAGGTGGGGAATGTTACTTCGTTTTCATGAAGTATGATCCTGAATATGAACGTCTTCGGGCTGATCGGTTAGTAAAAGTTGTCGACATAAACGTCTGAAAAAAAATGTTTTTCTTTTCTTCTTTAACATGTATTTTTTTTTTTAATTTGATATTTTTGGTATGAAGATGTGGGGAAGGGGCAGATGAGGTTGATGGGTACCTGAGTAGAAAGCACGATGAGGTTCTGAGGAGGTGGTTGGAGCCTGGCAGTTACAGAAAAATTTCGTCTTTTCTCATCGTTGATGGGTTTTCAGTCGAGATTAACGAACATCAGGTATTTCAAGTTTCGTTTAATAATGATTTTTTAAGAGAATATGCATATAAGATTATCAATACTTCTATGTTCTTAGCAGTATATATATGCTTTTTGGAAAAATGAAAAACTTAGAAACTAAAGGAAATGGTAAATTTTAGCTAGAAACAAAACTCGAACAATTACATATGGTATTTCTTTCTTCTTTTTCCGGTTTGTTATTAAAAGAAAAGAAAATTAACTTCAGCTTTCTTTTCCCACATAAAGAGTTTAATTTTTTAAAAGAAAAAGCCTTGAAAAGTTATTAAAAATAATAGATACAACAGAGGAAAATTATTGTAAATATTCTGTTAAGAAAAAAATGATTAAACATAGATGAACTTTTATTTATTATTTTGCTATATTTGAAAATTCTCTTTTGGAAAAAAAAAGAAGAAGAAGAAAAGAAAATGAGATTTGGTTTTGATGATTTAAATACAGGCAAATGTGCTTAGAAGTGCAGAGGGAGTAAGGGTGGTGGAGAAGAATCAAGACCAACCTCTCATTGTTAATCCAACATCAACTGATTAAAGGTAATAATTGAAAGAGCAAGGCCAAAAAAATACAAATAAATAAATTAATTAAAGAATAATCATCTTTGGAATTTTCTAAATCAAATCTTTGTATCCTTATTGTCAGTACTCTTTTAGTCTTTTGTGT

mRNA sequence

GATGCTCTCCTCTTCAGTAACCAAAATTAAACTTTATATTTTTAAACAAATAAATCTAACCGACCCTAATTAAATTATACTTACATCATATAGATATATATATGTGTGTGTGTAGTAGTTTATGCATTATTAAAGAGACTTCAATATTTTCCCTAAGGAAGAAAACTACAAAATGCAGGAGAACAGATACAACGGAGCACATGAACTCAGTGGCGGTGGTGGTGGGGGCAGAGGTGGGGAATGTTACTTCGTTTTCATGAAGTATGATCCTGAATATGAACGTCTTCGGGCTGATCGATGTGGGGAAGGGGCAGATGAGGTTGATGGGTACCTGAGTAGAAAGCACGATGAGGTTCTGAGGAGGTGGTTGGAGCCTGGCAGTTACAGAAAAATTTCGTCTTTTCTCATCGTTGATGGGTTTTCAGTCGAGATTAACGAACATCAGGCAAATGTGCTTAGAAGTGCAGAGGGAGTAAGGGTGGTGGAGAAGAATCAAGACCAACCTCTCATTGTTAATCCAACATCAACTGATTAAAGGTAATAATTGAAAGAGCAAGGCCAAAAAAATACAAATAAATAAATTAATTAAAGAATAATCATCTTTGGAATTTTCTAAATCAAATCTTTGTATCCTTATTGTCAGTACTCTTTTAGTCTTTTGTGT

Coding sequence (CDS)

ATGCAGGAGAACAGATACAACGGAGCACATGAACTCAGTGGCGGTGGTGGTGGGGGCAGAGGTGGGGAATGTTACTTCGTTTTCATGAAGTATGATCCTGAATATGAACGTCTTCGGGCTGATCGATGTGGGGAAGGGGCAGATGAGGTTGATGGGTACCTGAGTAGAAAGCACGATGAGGTTCTGAGGAGGTGGTTGGAGCCTGGCAGTTACAGAAAAATTTCGTCTTTTCTCATCGTTGATGGGTTTTCAGTCGAGATTAACGAACATCAGGCAAATGTGCTTAGAAGTGCAGAGGGAGTAAGGGTGGTGGAGAAGAATCAAGACCAACCTCTCATTGTTAATCCAACATCAACTGATTAA

Protein sequence

MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDEVLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD
Homology
BLAST of Cmc07g0198351 vs. NCBI nr
Match: KAA0044911.1 (subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa] >TYK16559.1 subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa])

HSP 1 Score: 251.1 bits (640), Expect = 4.7e-63
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120

BLAST of Cmc07g0198351 vs. NCBI nr
Match: XP_008451935.1 (PREDICTED: uncharacterized protein LOC103493088 [Cucumis melo])

HSP 1 Score: 250.0 bits (637), Expect = 1.0e-62
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSA+GVRVVEKNQDQPLIVNPTSTD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSADGVRVVEKNQDQPLIVNPTSTD 120

BLAST of Cmc07g0198351 vs. NCBI nr
Match: XP_011653213.1 (uncharacterized protein LOC105435187 [Cucumis sativus] >KAE8649203.1 hypothetical protein Csa_015401 [Cucumis sativus])

HSP 1 Score: 227.6 bits (579), Expect = 5.6e-56
Identity = 110/120 (91.67%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1   MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLR AEGVRVVEKNQDQPLI+NPT+TD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRYAEGVRVVEKNQDQPLIINPTTTD 115

BLAST of Cmc07g0198351 vs. NCBI nr
Match: XP_038877291.1 (uncharacterized protein LOC120069570 [Benincasa hispida])

HSP 1 Score: 204.1 bits (518), Expect = 6.6e-49
Identity = 101/120 (84.17%), Postives = 105/120 (87.50%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRY+G HE  GGGGGG GGECYFVFM YDPEYERLRADR GEG DE+D YLSRKHDE
Sbjct: 25  MQENRYDGGHEQGGGGGGGCGGECYFVFMNYDPEYERLRADRSGEGVDELDLYLSRKHDE 84

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           +L RWLEPGSYRKISSFLIVDGFSVEI E QANVLR AEGVRVVEKNQDQPLI N TS +
Sbjct: 85  ILGRWLEPGSYRKISSFLIVDGFSVEITEDQANVLRCAEGVRVVEKNQDQPLI-NGTSRE 143

BLAST of Cmc07g0198351 vs. NCBI nr
Match: XP_022931457.1 (subtilisin-like protease SBT2.5 [Cucurbita moschata])

HSP 1 Score: 172.2 bits (435), Expect = 2.8e-39
Identity = 88/110 (80.00%), Postives = 91/110 (82.73%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRY       GGGGGG GG CYFVFM YDPEYERLRADR GEGA E+D YLSRKHD 
Sbjct: 1   MQENRY-------GGGGGGGGGACYFVFMNYDPEYERLRADRSGEGAHELDEYLSRKHDG 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQ 111
           VL R LEPG+YRK+SSFLIVDGFSVEI E QA VLRSAEGVRVVEKNQDQ
Sbjct: 61  VLGRCLEPGTYRKVSSFLIVDGFSVEITEEQAYVLRSAEGVRVVEKNQDQ 103

BLAST of Cmc07g0198351 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 9.3e-06
Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = 0

Query: 53  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRSAEGVRVVEKN 108
           +L RKHD +L    E GSY+K+ S+  +++GF+  ++  QA  LR A GVR V+K+
Sbjct: 65  HLERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKD 120

BLAST of Cmc07g0198351 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-04
Identity = 21/56 (37.50%), Postives = 37/56 (66.07%), Query Frame = 0

Query: 53  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRSAEGVRVVEKN 108
           +L RKHD +L      GSY+K+ S+  +++GF+  ++  QA +LR A GV+ V+++
Sbjct: 65  HLERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRD 120

BLAST of Cmc07g0198351 vs. ExPASy TrEMBL
Match: A0A5D3D232 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003780 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.3e-63
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120

BLAST of Cmc07g0198351 vs. ExPASy TrEMBL
Match: A0A1S3BSM3 (uncharacterized protein LOC103493088 OS=Cucumis melo OX=3656 GN=LOC103493088 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.1e-63
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE
Sbjct: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQPLIVNPTSTD 120
           VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSA+GVRVVEKNQDQPLIVNPTSTD
Sbjct: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSADGVRVVEKNQDQPLIVNPTSTD 120

BLAST of Cmc07g0198351 vs. ExPASy TrEMBL
Match: A0A0A0KZT9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G051480 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.2e-41
Identity = 84/91 (92.31%), Postives = 85/91 (93.41%), Query Frame = 0

Query: 1  MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
          MQENRYNGAHEL     GGRG ECYFVFMKYDPEYERLRADRCGEGADE+DGYLSRKHDE
Sbjct: 1  MQENRYNGAHEL-----GGRGEECYFVFMKYDPEYERLRADRCGEGADELDGYLSRKHDE 60

Query: 61 VLRRWLEPGSYRKISSFLIVDGFSVEINEHQ 92
          VLRRWLEPGSYRKISSFLIVDGFSVEINEHQ
Sbjct: 61 VLRRWLEPGSYRKISSFLIVDGFSVEINEHQ 86

BLAST of Cmc07g0198351 vs. ExPASy TrEMBL
Match: A0A6J1ETN3 (subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111437632 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.3e-39
Identity = 88/110 (80.00%), Postives = 91/110 (82.73%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRY       GGGGGG GG CYFVFM YDPEYERLRADR GEGA E+D YLSRKHD 
Sbjct: 1   MQENRY-------GGGGGGGGGACYFVFMNYDPEYERLRADRSGEGAHELDEYLSRKHDG 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQ 111
           VL R LEPG+YRK+SSFLIVDGFSVEI E QA VLRSAEGVRVVEKNQDQ
Sbjct: 61  VLGRCLEPGTYRKVSSFLIVDGFSVEITEEQAYVLRSAEGVRVVEKNQDQ 103

BLAST of Cmc07g0198351 vs. ExPASy TrEMBL
Match: A0A6J1J855 (subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111483437 PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.7e-39
Identity = 86/110 (78.18%), Postives = 91/110 (82.73%), Query Frame = 0

Query: 1   MQENRYNGAHELSGGGGGGRGGECYFVFMKYDPEYERLRADRCGEGADEVDGYLSRKHDE 60
           MQENRY+G        GGG GG CYFVFM YDPEYERLRADR GEGA E+D YLSRKHD 
Sbjct: 1   MQENRYDGV-------GGGGGGACYFVFMNYDPEYERLRADRSGEGAHELDEYLSRKHDG 60

Query: 61  VLRRWLEPGSYRKISSFLIVDGFSVEINEHQANVLRSAEGVRVVEKNQDQ 111
           VL R LEPG+YRK+SSFLIVDGFSVEI E QA+VLRSAEGVRVVEKNQDQ
Sbjct: 61  VLGRCLEPGTYRKVSSFLIVDGFSVEITEEQADVLRSAEGVRVVEKNQDQ 103

BLAST of Cmc07g0198351 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 51.2 bits (121), Expect = 6.6e-07
Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = 0

Query: 53  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRSAEGVRVVEKN 108
           +L RKHD +L    E GSY+K+ S+  +++GF+  ++  QA  LR A GVR V+K+
Sbjct: 65  HLERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKD 120

BLAST of Cmc07g0198351 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.6e-05
Identity = 21/56 (37.50%), Postives = 37/56 (66.07%), Query Frame = 0

Query: 53  YLSRKHDEVLRRWLEPGSYRKISSFL-IVDGFSVEINEHQANVLRSAEGVRVVEKN 108
           +L RKHD +L      GSY+K+ S+  +++GF+  ++  QA +LR A GV+ V+++
Sbjct: 65  HLERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRD 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0044911.14.7e-63100.00subtilisin-like protease SBT2.5 [Cucumis melo var. makuwa] >TYK16559.1 subtilisi... [more]
XP_008451935.11.0e-6299.17PREDICTED: uncharacterized protein LOC103493088 [Cucumis melo][more]
XP_011653213.15.6e-5691.67uncharacterized protein LOC105435187 [Cucumis sativus] >KAE8649203.1 hypothetica... [more]
XP_038877291.16.6e-4984.17uncharacterized protein LOC120069570 [Benincasa hispida][more]
XP_022931457.12.8e-3980.00subtilisin-like protease SBT2.5 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O644819.3e-0642.86Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S... [more]
Q9SZV55.1e-0437.50Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A5D3D2322.3e-63100.00Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BSM35.1e-6399.17uncharacterized protein LOC103493088 OS=Cucumis melo OX=3656 GN=LOC103493088 PE=... [more]
A0A0A0KZT94.2e-4192.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G051480 PE=4 SV=1[more]
A0A6J1ETN31.3e-3980.00subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111437632 PE... [more]
A0A6J1J8558.7e-3978.18subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111483437 PE=4... [more]
Match NameE-valueIdentityDescription
AT2G19170.16.6e-0742.86subtilisin-like serine protease 3 [more]
AT4G30020.13.6e-0537.50PA-domain containing subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 53..108
e-value: 6.9E-6
score: 26.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR37379OS01G0220500 PROTEINcoord: 22..109

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc07g0198351.1Cmc07g0198351.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008233 peptidase activity