Cmc06g0176591 (gene) Melon (Charmono) v1.1

Overview
NameCmc06g0176591
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCMiso1.1chr06: 33362288 .. 33362873 (+)
RNA-Seq ExpressionCmc06g0176591
SyntenyCmc06g0176591
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAGAAATGAAATCCTCATATTTTCCTCTTCCAGTCAAAGCCATTCTTCTTCTCATGATCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAACAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCCGCCGCCGCCATTCACAGTAGCTATCGTCTAATGACCATCGCCGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGGTCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGAGGGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTATAGAGGAGTTGAAGGATAATAACAAGACTCGATTCGAAACTGAGGATATTCGGACGTGGGTTAGCGCTGCTCTAACCGACGACGATACATGTATGGATGGGTTGGTTGGAGATGCCATGAATGGTAACATAAAGGAATCGATAAAGGCAATGGTGGTGGATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTAA

mRNA sequence

GCAAGAAATGAAATCCTCATATTTTCCTCTTCCAGTCAAAGCCATTCTTCTTCTCATGATCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAACAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCCGCCGCCGCCATTCACAGTAGCTATCGTCTAATGACCATCGCCGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGGTCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGAGGGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTATAGAGGAGTTGAAGGATAATAACAAGACTCGATTCGAAACTGAGGATATTCGGACGTGGGTTAGCGCTGCTCTAACCGACGACGATACATGTATGGATGGGTTGGTTGGAGATGCCATGAATGGTAACATAAAGGAATCGATAAAGGCAATGGTGGTGGATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTAA

Coding sequence (CDS)

ATGAAATCCTCATATTTTCCTCTTCCAGTCAAAGCCATTCTTCTTCTCATGATCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAACAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCCGCCGCCGCCATTCACAGTAGCTATCGTCTAATGACCATCGCCGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGGTCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGAGGGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTATAGAGGAGTTGAAGGATAATAACAAGACTCGATTCGAAACTGAGGATATTCGGACGTGGGTTAGCGCTGCTCTAACCGACGACGATACATGTATGGATGGGTTGGTTGGAGATGCCATGAATGGTAACATAAAGGAATCGATAAAGGCAATGGTGGTGGATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTAA

Protein sequence

MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSLVSLLK
Homology
BLAST of Cmc06g0176591 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 357.1 bits (915), Expect = 9.7e-95
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
           MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

BLAST of Cmc06g0176591 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 321.6 bits (823), Expect = 4.5e-84
Identity = 175/193 (90.67%), Postives = 184/193 (95.34%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSN-IANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAA 60
           MKSSYFPLPVKAILL+ +LINQSN IANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA
Sbjct: 1   MKSSYFPLPVKAILLI-LLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  AIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEEL 120
            IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEEL
Sbjct: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120

Query: 121 KMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQ 180
           KMA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQ
Sbjct: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180

Query: 181 LTSIALSLVSLLK 193
           LTSIALSLVSLLK
Sbjct: 181 LTSIALSLVSLLK 192

BLAST of Cmc06g0176591 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 288.5 bits (737), Expect = 4.2e-74
Identity = 156/192 (81.25%), Postives = 169/192 (88.02%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           M+SSY PLP+KAILL+ ILINQSNIA SQP NDTQFIKTTCQSTPYP LCLSSLS  AA 
Sbjct: 1   MESSYLPLPIKAILLI-ILINQSNIAKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAAT 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           IH SYRLMTIAALTVALT TRS SS ++SLAK SN LTPREGYV+ DCIEEFGDS+EELK
Sbjct: 61  IHGSYRLMTIAALTVALTRTRSASSVIQSLAK-SNVLTPREGYVVSDCIEEFGDSIEELK 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
           MAI ELKDN+K RFE EDIRTWVSAALTDDDTCMDG VGD MN ++KES+K +VVD+ QL
Sbjct: 121 MAIVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 190

BLAST of Cmc06g0176591 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.0 bits (650), Expect = 5.2e-64
Identity = 139/185 (75.14%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 8   LPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRL 67
           LP+ AI L MILINQS    +QP NDTQFIKTTCQ+TPYPDLCLSSLS  A+AIH S+RL
Sbjct: 6   LPITAI-LFMILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRL 65

Query: 68  MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 127
           MTIAAL VALTHT   SS V SLAK SNALTPREGY + DCIEEFGDS+EELKMA+EELK
Sbjct: 66  MTIAALAVALTHTHDASSTVTSLAK-SNALTPREGYALSDCIEEFGDSIEELKMAVEELK 125

Query: 128 DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 187
            N+KTR + +DI+TWVSAALTDD+TCMDG   DAMNG++KES++A+VVDVAQLTSIALSL
Sbjct: 126 GNDKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSL 185

Query: 188 VSLLK 193
           V  LK
Sbjct: 186 VRQLK 188

BLAST of Cmc06g0176591 vs. NCBI nr
Match: XP_022923321.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 251.5 bits (641), Expect = 5.7e-63
Identity = 138/192 (71.88%), Postives = 158/192 (82.29%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           M+SS  P+     ++ +ILINQS    +QP NDTQFIKTTCQ+TPYPDLCLSSLS  A+A
Sbjct: 1   MESSVLPI---TAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASA 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           I  S+RLMTIAAL VALTHT   SS V SLAK SNA+T REGY + DCIEEFGDSVEELK
Sbjct: 61  IRCSHRLMTIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELK 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
           MA+E LK N+KTR +TEDI+TW+SAALTDD+TCMDG  GDAMNG++KES+KAMVVDVAQL
Sbjct: 121 MAVEALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLV  LK
Sbjct: 181 TSIALSLVRQLK 188

BLAST of Cmc06g0176591 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.2e-24
Identity = 77/194 (39.69%), Postives = 113/194 (58.25%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           MK S   L   A+L+++        A++ P N  QFIKT+C  T YP +C  SLS  A  
Sbjct: 1   MKLSKSTLVFSALLVILA------AASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKT 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           I ++ + +   AL V+LT T+   + ++ L K    L  R+   I DC+EE  DS++ + 
Sbjct: 61  IQNNPQELASTALQVSLTRTQQAQTFMKRLNK-FKGLKARQYAAIHDCLEEVEDSLDRVS 120

Query: 121 MAIEELKD-----NNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVV 180
            + +E+K+      N   F   ++ TWVSAALTD+ TCMDG  G  M+G IKES++A VV
Sbjct: 121 RSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVV 180

Query: 181 DVAQLTSIALSLVS 190
            VA++TS AL+LV+
Sbjct: 181 AVARVTSNALALVN 185

BLAST of Cmc06g0176591 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.9e-23
Identity = 71/184 (38.59%), Postives = 108/184 (58.70%), Query Frame = 0

Query: 13  ILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAI-HSSYRLMTIA 72
           ++LL I I QS+ A    +   QFI ++CQ+T YP LC+ +LS  A  I H++ + +   
Sbjct: 10  VILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQT 69

Query: 73  ALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK---- 132
           AL ++L   +S +  V  L K +     RE   I+DCIE  G+SV+ L  +++EL     
Sbjct: 70  ALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGH 129

Query: 133 --DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIAL 190
              +    ++  +++TWVSAALTD+ TC+DG    AM G +K  I+  VV VAQ+TS AL
Sbjct: 130 AVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNAL 189

BLAST of Cmc06g0176591 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 63/167 (37.72%), Postives = 95/167 (56.89%), Query Frame = 0

Query: 29  QPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRLMTIAALTVALTHTRSTSSAVE 88
           + + DT+FIK +C++T YPD C  SLS  A+ I    R +   AL V++   +S  + V 
Sbjct: 30  ESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVS 89

Query: 89  SLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK------DNNKTRFETEDIRTW 148
            +      +T R+   + DC+EE GD+V+ L  +++ELK            F   ++RTW
Sbjct: 90  EMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTW 149

Query: 149 VSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSLVS 190
            SAALTD+  CMDG  G AM G +K  I+  +V VA+ TS AL+L++
Sbjct: 150 TSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALIN 195

BLAST of Cmc06g0176591 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.3e-21
Identity = 73/193 (37.82%), Postives = 111/193 (57.51%), Query Frame = 0

Query: 3   SSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIH 62
           SS  P P  +  L  +  + S+   S  TN   +IKT+C  T Y  +C +SLS  A+ I 
Sbjct: 115 SSPPPPPPSSSPLSSLSPSSSSSTYSNQTN-LDYIKTSCNITLYKTICYNSLSPYASTIR 174

Query: 63  SSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMA 122
           S+ + + + AL + L+  +S S  V++++     LT  E   + DC+EE GDSV  L+ +
Sbjct: 175 SNPQKLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDS 234

Query: 123 IEEL-----KDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDV 182
           I EL     KD+ K      D+ TWVSAALT+DDTCMDG     +   +K+ ++  VV+V
Sbjct: 235 IRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEV 294

Query: 183 AQLTSIALSLVSL 191
           A+LTS AL+L+++
Sbjct: 295 ARLTSNALALINM 303

BLAST of Cmc06g0176591 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 57/186 (30.65%), Postives = 102/186 (54.84%), Query Frame = 0

Query: 14  LLLMILINQSNIANSQPT------NDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRL 73
           L LM+ IN SN   +  T      N T ++KT C ST YP +C + LS  ++ I S    
Sbjct: 9   LTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIK 68

Query: 74  MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 133
           +   +L + +   ++ +  V +L + + A    E  +++DC++E  D+++ELK A+ E+K
Sbjct: 69  LCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMK 128

Query: 134 ----DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSI 190
                   T    ++++TWVS+ALTD+ TC DG     +N   K+ +K  + ++++ TS 
Sbjct: 129 YVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEGRVNVETKKKVKKAISELSKTTSN 188

BLAST of Cmc06g0176591 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.7e-95
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
           MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

BLAST of Cmc06g0176591 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.2e-84
Identity = 175/193 (90.67%), Postives = 184/193 (95.34%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSN-IANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAA 60
           MKSSYFPLPVKAILL+ +LINQSN IANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA
Sbjct: 1   MKSSYFPLPVKAILLI-LLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAA 60

Query: 61  AIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEEL 120
            IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEEL
Sbjct: 61  TIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEEL 120

Query: 121 KMAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQ 180
           KMA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQ
Sbjct: 121 KMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQ 180

Query: 181 LTSIALSLVSLLK 193
           LTSIALSLVSLLK
Sbjct: 181 LTSIALSLVSLLK 192

BLAST of Cmc06g0176591 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.8e-63
Identity = 138/192 (71.88%), Postives = 158/192 (82.29%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           M+SS  P+     ++ +ILINQS    +QP NDTQFIKTTCQ+TPYPDLCLSSLS  A+A
Sbjct: 1   MESSVLPI---TAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASA 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           I  S+RLMTIAAL VALTHT   SS V SLAK SNA+T REGY + DCIEEFGDSVEELK
Sbjct: 61  IRCSHRLMTIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELK 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
           MA+E LK N+KTR +TEDI+TW+SAALTDD+TCMDG  GDAMNG++KES+KAMVVDVAQL
Sbjct: 121 MAVEALKGNDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLV  LK
Sbjct: 181 TSIALSLVRQLK 188

BLAST of Cmc06g0176591 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.0e-57
Identity = 133/192 (69.27%), Postives = 154/192 (80.21%), Query Frame = 0

Query: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60
           M+ SYFPLP+ AILL+ ILIN SN       +DTQFIK TCQ+TPYPDLC SSLS  A +
Sbjct: 1   MEDSYFPLPITAILLI-ILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVS 60

Query: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120
           +H S R MTIAALTVALTHTRS SS V SLAK SNAL P+E + +RDCIEE GDSVEEL+
Sbjct: 61  VHGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQ 120

Query: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180
            ++ ELKD + +R ET+DIRTWVSAALTDDDTCM+GLVGD   G +KES++ +VVDVAQL
Sbjct: 121 SSVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQL 180

Query: 181 TSIALSLVSLLK 193
           TSIALSLVS LK
Sbjct: 181 TSIALSLVSHLK 184

BLAST of Cmc06g0176591 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 5.1e-57
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0

Query: 68  MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 127
           MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 128 DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 187
           DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 188 VSLLK 193
           VSLLK
Sbjct: 121 VSLLK 125

BLAST of Cmc06g0176591 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-28
Identity = 71/179 (39.66%), Postives = 116/179 (64.80%), Query Frame = 0

Query: 13  ILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRLMTIAA 72
           I+ ++ L++ +    +  T +T+F+K++C  T YP LC SSLS  A+ I +S +LM  AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 73  LTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELKDNNKT 132
           L + L   + TS+ +  L  S++ L P+E   +RDC+EE GD++EEL+ +I E+   + +
Sbjct: 80  LNITLASAKVTSAMMVRL--SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGS 139

Query: 133 RFET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSLVS 190
            +E    DI+TWVSAALTD +TC DG  G+ M+G +K  ++  ++ +A LTS AL+L++
Sbjct: 140 NYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALIN 196

BLAST of Cmc06g0176591 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 5.6e-24
Identity = 71/184 (38.59%), Postives = 108/184 (58.70%), Query Frame = 0

Query: 13  ILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAI-HSSYRLMTIA 72
           ++LL I I QS+ A    +   QFI ++CQ+T YP LC+ +LS  A  I H++ + +   
Sbjct: 10  VILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQT 69

Query: 73  ALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK---- 132
           AL ++L   +S +  V  L K +     RE   I+DCIE  G+SV+ L  +++EL     
Sbjct: 70  ALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGH 129

Query: 133 --DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIAL 190
              +    ++  +++TWVSAALTD+ TC+DG    AM G +K  I+  VV VAQ+TS AL
Sbjct: 130 AVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNAL 189

BLAST of Cmc06g0176591 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 63/167 (37.72%), Postives = 95/167 (56.89%), Query Frame = 0

Query: 29  QPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRLMTIAALTVALTHTRSTSSAVE 88
           + + DT+FIK +C++T YPD C  SLS  A+ I    R +   AL V++   +S  + V 
Sbjct: 30  ESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVS 89

Query: 89  SLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK------DNNKTRFETEDIRTW 148
            +      +T R+   + DC+EE GD+V+ L  +++ELK            F   ++RTW
Sbjct: 90  EMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTW 149

Query: 149 VSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSLVS 190
            SAALTD+  CMDG  G AM G +K  I+  +V VA+ TS AL+L++
Sbjct: 150 TSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALIN 195

BLAST of Cmc06g0176591 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-22
Identity = 62/160 (38.75%), Postives = 97/160 (60.62%), Query Frame = 0

Query: 36  FIKTTCQSTPYPDLCLSSLSDSAAAIHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSN 95
           FI+++C++T YP LC+ SLS  A  I +S + +   A+ V L+  +ST   V  L +   
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTR-MK 97

Query: 96  ALTPREGYVIRDCIEEFGDSVEELKMAIEEL------KDNNKTRFETEDIRTWVSAALTD 155
            L  RE   I+DC+EE  D+V+ L  +++EL      KD ++  +   + +TW SAALTD
Sbjct: 98  GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTD 157

Query: 156 DDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSLVS 190
           ++TC DG  G  M+G IK S++A +++V   TS ALSL++
Sbjct: 158 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

BLAST of Cmc06g0176591 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-22
Identity = 73/193 (37.82%), Postives = 111/193 (57.51%), Query Frame = 0

Query: 3   SSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAAIH 62
           SS  P P  +  L  +  + S+   S  TN   +IKT+C  T Y  +C +SLS  A+ I 
Sbjct: 115 SSPPPPPPSSSPLSSLSPSSSSSTYSNQTN-LDYIKTSCNITLYKTICYNSLSPYASTIR 174

Query: 63  SSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMA 122
           S+ + + + AL + L+  +S S  V++++     LT  E   + DC+EE GDSV  L+ +
Sbjct: 175 SNPQKLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDS 234

Query: 123 IEEL-----KDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDV 182
           I EL     KD+ K      D+ TWVSAALT+DDTCMDG     +   +K+ ++  VV+V
Sbjct: 235 IRELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEV 294

Query: 183 AQLTSIALSLVSL 191
           A+LTS AL+L+++
Sbjct: 295 ARLTSNALALINM 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008448897.19.7e-95100.00PREDICTED: 21 kDa protein-like [Cucumis melo][more]
XP_004147977.14.5e-8490.6721 kDa protein [Cucumis sativus][more]
XP_038876812.14.2e-7481.2521 kDa protein-like [Benincasa hispida][more]
XP_023551923.15.2e-6475.1421 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_022923321.15.7e-6371.8821 kDa protein-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P174074.2e-2439.6921 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SI727.9e-2338.59Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Q9SB371.8e-2237.72Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
Q9SI744.3e-2137.82Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
Q9SB381.8e-1930.65Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BLP34.7e-95100.0021 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1[more]
A0A0A0L1P32.2e-8490.67PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S... [more]
A0A6J1E6H92.8e-6371.8821 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1[more]
A0A6J1DFW83.0e-5769.27pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P... [more]
A0A5D3D7I05.1e-57100.0021 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G... [more]
Match NameE-valueIdentityDescription
AT5G62360.11.7e-2839.66Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G62770.15.6e-2438.59Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G25260.11.2e-2337.72Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62350.11.8e-2238.75Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G62760.13.1e-2237.82Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 109..129
NoneNo IPR availablePANTHERPTHR31080:SF16121 KDA PROTEIN-LIKEcoord: 13..189
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 13..189
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 39..191
e-value: 3.22312E-37
score: 124.473
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 32..187
e-value: 7.5E-43
score: 158.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 36..187
e-value: 7.2E-36
score: 123.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 13..189
e-value: 1.2E-30
score: 104.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 31..192
e-value: 1.8E-38
score: 133.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 35..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc06g0176591.1Cmc06g0176591.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity