Cmc06g0161441 (gene) Melon (Charmono) v1.1

Overview
NameCmc06g0161441
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSerine hydroxymethyltransferase
LocationCMiso1.1chr06: 9862621 .. 9864169 (-)
RNA-Seq ExpressionCmc06g0161441
SyntenyCmc06g0161441
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACACGAAGGAGATTTGTACGCTCGTGGGGTAATCAGACCCTTCGATCTGTGGATCCTAGGTTGTATGGGATTATAAAGAAAGAGAAACAGTTCCAAATCAATGGCATTGAATTGTTTGCTTCTGAAAATTATGTGCGTCGAGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCAAAAGGTATTCCTGGTGCGAGGTACTACGGTGGAAATCAATATATAGGTGAGATTGAAATCCTTTGTCATGAGCGCGCATTTTGGCAGCATTTGATATTAATCCTCACACGTGGGGAGTTAACGTTCAACCTTATTCATGCACGTCTGCAAATTTTGCCGTCTAAACTGGTTATATAGATTATGATAAGCTTGAGGAGAGGGCGCTTGCCTTTTGCCCTAAACTACTTATCTGTGGAGGGAGCGCGTATCCTCGTGAATTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTTTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCTAAGGTGTGAATTTTTCGTCATCTTTAGCGATGTACTATCCCTTTAATGTCTGTGTAAAAAGAAAATTTGGTTCGGGTCCACAAAGTTCTTGGTTTTCTTATCATATGTTCTAGTTACTATGACGTTCTCATTCTCTTGGGTTGCAATTTAGCTGGTTGTTAATATATTCATAATAATTGAATTGTTCTACCTGACACTCAGAAAAGGCTTCAAAGGTGGTTGTGTGGAAATTTATTGTAGTTTTTAGCATTTAGGTTATGAATGGCTATGAAACTTAGTGATAGGAAAATGTAACTTATCGAGAGATTATGGTAGAGACTGATTTTAGGACTTAACCCTAGTTTACAAGTGCGTTGGGCCAAGATGTCAACCCCATGGATATTTTTTTTTTTGACCAAAACGATTTGGTATTTATTCTTTCATGGTGGATATGTATTTATCAGAAAAACTGAAAGCTTGACCAACATCTATTATTTTTCTCAGCAGTAGGATGTTATTTTTTCTCTTCCTTTTCTACTTGGTTCTAATATACAGTTCCGTTCAACTGTGTGCTTATGTAGTTCTCAGTATTATTCGTTTACATGTGTAATTGTTTCTTCACTTACATTGTTTGGTCCAGTTCATCAGTACTTCATACATGTGATCTTGTAATTTTGAATGAAGAATTGTCTCCATTCTGTTTATACTTTGATTGTGATTTAAAATTTTGTTACAGGAATGTGCGAGTCTTTTTGAGTACTGTGATGTTGTGACTTCAACTACTCATAAGAGCCTTCGAGGTCCAAGGCGAGGTATAATTTTTTTATAAGAAGGGTTGTGAGCCAAGGGGAAGTGAAATTGTTCTGTATCAGGTCAAGGAATATGACTTTGAGGAGAGGATAAACTTTGCTGTTTTTACATCCTTACAAGGTGGACCGCACAATAACCATATTGCAGCTCTTGCTGTGGCCCTAAAACAAGTGGCTACTCTAGAGTTTATTTTGAATCGGCAAATTTCATCTCTCTGTTAA

mRNA sequence

ATGGAGACACGAAGGAGATTTGTACGCTCGTGGGGTAATCAGACCCTTCGATCTGTGGATCCTAGGTTGTATGGGATTATAAAGAAAGAGAAACAGTTCCAAATCAATGGCATTGAATTGTTTGCTTCTGAAAATTATGTGCGTCGAGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCAAAAGGTATTCCTGGTGCGAGGTACTACGGTGGAAATCAATATATAGGTGAGATTGAAATCCTTTGTCATGAGCGCGCATTTTGGCAGCATTTGATATTAATCCTCACACGTGGGGAGTTAACGGCGCTTGCCTTTTGCCCTAAACTACTTATCTGTGGAGGGAGCGCGTATCCTCGTGAATTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTTTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCTAAGGTCAAGGAATATGACTTTGAGGAGAGGATAAACTTTGCTGTTTTTACATCCTTACAAGGTGGACCGCACAATAACCATATTGCAGCTCTTGCTGTGGCCCTAAAACAAGTGGCTACTCTAGAGTTTATTTTGAATCGGCAAATTTCATCTCTCTGTTAA

Coding sequence (CDS)

ATGGAGACACGAAGGAGATTTGTACGCTCGTGGGGTAATCAGACCCTTCGATCTGTGGATCCTAGGTTGTATGGGATTATAAAGAAAGAGAAACAGTTCCAAATCAATGGCATTGAATTGTTTGCTTCTGAAAATTATGTGCGTCGAGCTGTAATGGAAGCACTTGGAAGCCACTTGACTAATAAGTACTCAAAAGGTATTCCTGGTGCGAGGTACTACGGTGGAAATCAATATATAGGTGAGATTGAAATCCTTTGTCATGAGCGCGCATTTTGGCAGCATTTGATATTAATCCTCACACGTGGGGAGTTAACGGCGCTTGCCTTTTGCCCTAAACTACTTATCTGTGGAGGGAGCGCGTATCCTCGTGAATTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTTTGGAGCAGTTCTGATGTGTGATATGGCTCAAATTAGTGGCCTTGTTGCTGCTAAGGTCAAGGAATATGACTTTGAGGAGAGGATAAACTTTGCTGTTTTTACATCCTTACAAGGTGGACCGCACAATAACCATATTGCAGCTCTTGCTGTGGCCCTAAAACAAGTGGCTACTCTAGAGTTTATTTTGAATCGGCAAATTTCATCTCTCTGTTAA

Protein sequence

METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVKEYDFEERINFAVFTSLQGGPHNNHIAALAVALKQVATLEFILNRQISSLC
Homology
BLAST of Cmc06g0161441 vs. NCBI nr
Match: TYK11173.1 (serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 309.3 bits (791), Expect = 2.5e-80
Identity = 165/213 (77.46%), Postives = 173/213 (81.22%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYSKGIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGSA
Sbjct: 61  NKYSKGIPGARYYGGNQYIDYDKL------------------EERALAFCPKLLICGGSA 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK--VKEYDFEERINFAVFTSL----Q 180
           YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK     +++ + +      SL    +
Sbjct: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKECASLFEYCDVVTSTTHKSLRGPRR 180

Query: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 208
           GGPHNNHIAALAVALKQVATLEFILNRQISSLC
Sbjct: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 195

BLAST of Cmc06g0161441 vs. NCBI nr
Match: KAA0038974.1 (serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 303.5 bits (776), Expect = 1.4e-78
Identity = 162/213 (76.06%), Postives = 170/213 (79.81%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYSKGIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGS 
Sbjct: 61  NKYSKGIPGARYYGGNQYIDYDKL------------------EERALAFCPKLLICGGST 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK--VKEYDFEERINFAVFTSL----Q 180
           YPRELDYARFRQ ADKFGAVLMCDMAQISGLVAAK     +++ + +      SL    +
Sbjct: 121 YPRELDYARFRQNADKFGAVLMCDMAQISGLVAAKECASLFEYCDVVTSTTHKSLRSPRR 180

Query: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 208
           GGPHNNHIAAL VALKQVATLEFILNRQISSLC
Sbjct: 181 GGPHNNHIAALVVALKQVATLEFILNRQISSLC 195

BLAST of Cmc06g0161441 vs. NCBI nr
Match: KAA0038985.1 (serine hydroxymethyltransferase 7-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 300.4 bits (768), Expect = 1.2e-77
Identity = 160/214 (74.77%), Postives = 166/214 (77.57%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  +RSWGNQTLRSVDP+LYGII+KEKQ QINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRSSIRSWGNQTLRSVDPKLYGIIEKEKQGQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYS GIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGS 
Sbjct: 61  NKYSNGIPGARYYGGNQYIDYEKL------------------EERALAFCPKLLICGGSV 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK-----------------VKEYDFEE 180
           YPRELDYARFRQIADK GAVLMCDMAQISGLVAAK                 VKEYDFEE
Sbjct: 121 YPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKKGCEPRGSEIVLYQGNDVKEYDFEE 180

Query: 181 RINFAVFTSLQGGPHNNHIAALAVALKQVATLEF 198
           RINFAVF SLQGGPHNNHIAALAVALKQVAT E+
Sbjct: 181 RINFAVFPSLQGGPHNNHIAALAVALKQVATPEY 196

BLAST of Cmc06g0161441 vs. NCBI nr
Match: XP_004146284.1 (serine hydroxymethyltransferase 7 [Cucumis sativus] >KGN57592.1 hypothetical protein Csa_010427 [Cucumis sativus])

HSP 1 Score: 270.4 bits (690), Expect = 1.3e-68
Identity = 169/320 (52.81%), Postives = 178/320 (55.62%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  VRSWGNQTLR+ DP+LYGI++KEKQ QINGIEL ASENYV RAVMEALGSHLT
Sbjct: 113 METRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLT 172

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYYGGNQYI EIEILC ERA                        F  +  
Sbjct: 173 NKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTG 232

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     TR                               E  
Sbjct: 233 LLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEER 292

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL F PKLLICGGSAYPRELDYARFRQIADK GAVLMCDMAQISGLVAAK          
Sbjct: 293 ALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCD 352

BLAST of Cmc06g0161441 vs. NCBI nr
Match: XP_016901913.1 (PREDICTED: serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo])

HSP 1 Score: 266.5 bits (680), Expect = 1.9e-67
Identity = 167/320 (52.19%), Postives = 177/320 (55.31%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  VRSWGNQTLR+ DP+LYGI++KEKQ QINGIEL ASENYV RAVMEALGSHLT
Sbjct: 113 METRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLT 172

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYYGGNQYI EIEILC ERA                        F  +  
Sbjct: 173 NKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTG 232

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     TR                               E  
Sbjct: 233 LLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEER 292

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL F PKLLICGGSAYPRELDYARFRQIADK GAVLMCDMAQISGLVAAK          
Sbjct: 293 ALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECASPFEYCD 352

BLAST of Cmc06g0161441 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-53
Identity = 134/322 (41.61%), Postives = 161/322 (50.00%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           +E+RR  VR+WGNQ++   DP ++  ++KEKQ Q  GIEL ASEN+V RAVMEALGSHLT
Sbjct: 127 IESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLT 186

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAF---------WQHLIL-------------- 120
           NKYS+G+PGARYY GNQYI +IEILC ERA          W   +               
Sbjct: 187 NKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTG 246

Query: 121 ILTRGEL----------------------------------------------------T 180
           +L  GE                                                      
Sbjct: 247 LLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEK 306

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVK-------- 200
           AL + PK+LICGGS+YPR+ ++ RFR IADK GAVLM DMAQISGLVAAK          
Sbjct: 307 ALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCD 366

BLAST of Cmc06g0161441 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.7e-53
Identity = 136/320 (42.50%), Postives = 161/320 (50.31%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           +E+RR  VR+WG+Q +   DP ++ +++KEKQ Q+ GIEL ASEN+V RAVMEALGSHLT
Sbjct: 123 LESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLT 182

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYY GNQYI +IE LC ERA                        F  +  
Sbjct: 183 NKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTG 242

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     T G                              E  
Sbjct: 243 LLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDK 302

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL + PK+LICGGS+YPR+ D+AR RQIADK GAVLMCDMA ISGLVA K          
Sbjct: 303 ALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCD 362

BLAST of Cmc06g0161441 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.6e-48
Identity = 128/308 (41.56%), Postives = 149/308 (48.38%), Query Frame = 0

Query: 8   VRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGI 67
           V SWGN +L SVDP ++ +I+KEK+ Q  GIEL ASEN+   AV+EALGS LTNKYS+GI
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 68  PGARYYGGNQYIGEIEILCHERA------------------------FWQHLILI----- 127
           PG RYYGGN++I EIE LC  RA                        F  +  L+     
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 128 -----------LTRGELT-----------------------------------ALAFCPK 187
                      LT G  T                                   AL F PK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 188 LLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVKE-------------- 198
           LLICGGSAYPR+ DYARFR IADK GA+L+CDMA ISGLVAA+                 
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

BLAST of Cmc06g0161441 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 6.3e-42
Identity = 116/308 (37.66%), Postives = 140/308 (45.45%), Query Frame = 0

Query: 8   VRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGI 67
           V SWGN  L  VDP +Y +I+KEK  Q  GIEL A+EN+   AVMEALGS LTNKYS+G+
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 68  PGARYYGGNQYIGEIEILCHERA------------------------FWQHLILI----- 127
           PG RYYGG ++I EIE LC  R+                        F  +  L+     
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 128 -----------LTRG-----------------------------------ELTALAFCPK 187
                      +T G                                   E  A+ F PK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 188 LLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKV---------------- 198
           L+ICGG++YPRE DYARFR +ADK GA L+CDMA  S LVAA+                 
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

BLAST of Cmc06g0161441 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.8e-41
Identity = 112/301 (37.21%), Postives = 137/301 (45.51%), Query Frame = 0

Query: 12  GNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGIPGAR 71
           GN  L+ VD  ++ ++ +EK  Q  G+EL ASEN+  RAVMEALGSH TNKY++G PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 72  YYGGNQYIGEIEILCHERA------------------------FWQHLILI--------- 131
           YYGG + + E+E LC +RA                        F  +  L+         
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 132 -------LTRG---------------------------------ELTALAFCPKLLICGG 191
                  LT G                                 E  AL F PKL+I G 
Sbjct: 126 DLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGLIDYQRLEENALLFKPKLIISGA 185

Query: 192 SAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAA------------------------ 198
           SAYPRE DY R R IADK GA LMCDMA  SGLVAA                        
Sbjct: 186 SAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLRGP 245

BLAST of Cmc06g0161441 vs. ExPASy TrEMBL
Match: A0A5D3CH07 (Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00060 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.2e-80
Identity = 165/213 (77.46%), Postives = 173/213 (81.22%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYSKGIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGSA
Sbjct: 61  NKYSKGIPGARYYGGNQYIDYDKL------------------EERALAFCPKLLICGGSA 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK--VKEYDFEERINFAVFTSL----Q 180
           YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK     +++ + +      SL    +
Sbjct: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKECASLFEYCDVVTSTTHKSLRGPRR 180

Query: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 208
           GGPHNNHIAALAVALKQVATLEFILNRQISSLC
Sbjct: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 195

BLAST of Cmc06g0161441 vs. ExPASy TrEMBL
Match: A0A5A7T654 (Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.7e-79
Identity = 162/213 (76.06%), Postives = 170/213 (79.81%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYSKGIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGS 
Sbjct: 61  NKYSKGIPGARYYGGNQYIDYDKL------------------EERALAFCPKLLICGGST 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK--VKEYDFEERINFAVFTSL----Q 180
           YPRELDYARFRQ ADKFGAVLMCDMAQISGLVAAK     +++ + +      SL    +
Sbjct: 121 YPRELDYARFRQNADKFGAVLMCDMAQISGLVAAKECASLFEYCDVVTSTTHKSLRSPRR 180

Query: 181 GGPHNNHIAALAVALKQVATLEFILNRQISSLC 208
           GGPHNNHIAAL VALKQVATLEFILNRQISSLC
Sbjct: 181 GGPHNNHIAALVVALKQVATLEFILNRQISSLC 195

BLAST of Cmc06g0161441 vs. ExPASy TrEMBL
Match: A0A5A7TC66 (Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G00660 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.6e-78
Identity = 160/214 (74.77%), Postives = 166/214 (77.57%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  +RSWGNQTLRSVDP+LYGII+KEKQ QINGIELFASENYVRRAVMEALGSHLT
Sbjct: 1   METRRSSIRSWGNQTLRSVDPKLYGIIEKEKQGQINGIELFASENYVRRAVMEALGSHLT 60

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAFWQHLILILTRGELTALAFCPKLLICGGSA 120
           NKYS GIPGARYYGGNQYI   ++                  E  ALAFCPKLLICGGS 
Sbjct: 61  NKYSNGIPGARYYGGNQYIDYEKL------------------EERALAFCPKLLICGGSV 120

Query: 121 YPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK-----------------VKEYDFEE 180
           YPRELDYARFRQIADK GAVLMCDMAQISGLVAAK                 VKEYDFEE
Sbjct: 121 YPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKKGCEPRGSEIVLYQGNDVKEYDFEE 180

Query: 181 RINFAVFTSLQGGPHNNHIAALAVALKQVATLEF 198
           RINFAVF SLQGGPHNNHIAALAVALKQVAT E+
Sbjct: 181 RINFAVFPSLQGGPHNNHIAALAVALKQVATPEY 196

BLAST of Cmc06g0161441 vs. ExPASy TrEMBL
Match: A0A0A0LAD1 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G221750 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 6.2e-69
Identity = 169/320 (52.81%), Postives = 178/320 (55.62%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  VRSWGNQTLR+ DP+LYGI++KEKQ QINGIEL ASENYV RAVMEALGSHLT
Sbjct: 113 METRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLT 172

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYYGGNQYI EIEILC ERA                        F  +  
Sbjct: 173 NKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTG 232

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     TR                               E  
Sbjct: 233 LLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEER 292

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL F PKLLICGGSAYPRELDYARFRQIADK GAVLMCDMAQISGLVAAK          
Sbjct: 293 ALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFEYCD 352

BLAST of Cmc06g0161441 vs. ExPASy TrEMBL
Match: A0A1S3C2G6 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496109 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 9.0e-68
Identity = 167/320 (52.19%), Postives = 177/320 (55.31%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           METRR  VRSWGNQTLR+ DP+LYGI++KEKQ QINGIEL ASENYV RAVMEALGSHLT
Sbjct: 113 METRRSSVRSWGNQTLRAADPKLYGIMEKEKQRQINGIELIASENYVCRAVMEALGSHLT 172

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYYGGNQYI EIEILC ERA                        F  +  
Sbjct: 173 NKYSEGMPGARYYGGNQYIDEIEILCRERALAAFDLNPHSWGVNVQPYSCTSANFAVYTG 232

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     TR                               E  
Sbjct: 233 LLLPGDRIMALDSPSGGNPSHGYCTRNGKKVAGASIFFESFPYKVNPQTGYIDYDKLEER 292

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL F PKLLICGGSAYPRELDYARFRQIADK GAVLMCDMAQISGLVAAK          
Sbjct: 293 ALDFRPKLLICGGSAYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECASPFEYCD 352

BLAST of Cmc06g0161441 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 211.1 bits (536), Expect = 8.6e-55
Identity = 134/322 (41.61%), Postives = 161/322 (50.00%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           +E+RR  VR+WGNQ++   DP ++  ++KEKQ Q  GIEL ASEN+V RAVMEALGSHLT
Sbjct: 127 IESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLT 186

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERAF---------WQHLIL-------------- 120
           NKYS+G+PGARYY GNQYI +IEILC ERA          W   +               
Sbjct: 187 NKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTG 246

Query: 121 ILTRGEL----------------------------------------------------T 180
           +L  GE                                                      
Sbjct: 247 LLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEK 306

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVK-------- 200
           AL + PK+LICGGS+YPR+ ++ RFR IADK GAVLM DMAQISGLVAAK          
Sbjct: 307 ALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCD 366

BLAST of Cmc06g0161441 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 209.9 bits (533), Expect = 1.9e-54
Identity = 136/320 (42.50%), Postives = 161/320 (50.31%), Query Frame = 0

Query: 1   METRRRFVRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLT 60
           +E+RR  VR+WG+Q +   DP ++ +++KEKQ Q+ GIEL ASEN+V RAVMEALGSHLT
Sbjct: 123 LESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLT 182

Query: 61  NKYSKGIPGARYYGGNQYIGEIEILCHERA------------------------FWQHLI 120
           NKYS+G+PGARYY GNQYI +IE LC ERA                        F  +  
Sbjct: 183 NKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTG 242

Query: 121 LIL---------------------TRG------------------------------ELT 180
           L+L                     T G                              E  
Sbjct: 243 LLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDK 302

Query: 181 ALAFCPKLLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAK---------- 198
           AL + PK+LICGGS+YPR+ D+AR RQIADK GAVLMCDMA ISGLVA K          
Sbjct: 303 ALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCD 362

BLAST of Cmc06g0161441 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 193.4 bits (490), Expect = 1.9e-49
Identity = 128/308 (41.56%), Postives = 149/308 (48.38%), Query Frame = 0

Query: 8   VRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGI 67
           V SWGN +L SVDP ++ +I+KEK+ Q  GIEL ASEN+   AV+EALGS LTNKYS+GI
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 68  PGARYYGGNQYIGEIEILCHERA------------------------FWQHLILI----- 127
           PG RYYGGN++I EIE LC  RA                        F  +  L+     
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 128 -----------LTRGELT-----------------------------------ALAFCPK 187
                      LT G  T                                   AL F PK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 188 LLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVKE-------------- 198
           LLICGGSAYPR+ DYARFR IADK GA+L+CDMA ISGLVAA+                 
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

BLAST of Cmc06g0161441 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 4.5e-43
Identity = 116/308 (37.66%), Postives = 140/308 (45.45%), Query Frame = 0

Query: 8   VRSWGNQTLRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGI 67
           V SWGN  L  VDP +Y +I+KEK  Q  GIEL A+EN+   AVMEALGS LTNKYS+G+
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 68  PGARYYGGNQYIGEIEILCHERA------------------------FWQHLILI----- 127
           PG RYYGG ++I EIE LC  R+                        F  +  L+     
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 128 -----------LTRG-----------------------------------ELTALAFCPK 187
                      +T G                                   E  A+ F PK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 188 LLICGGSAYPRELDYARFRQIADKFGAVLMCDMAQISGLVAAKV---------------- 198
           L+ICGG++YPRE DYARFR +ADK GA L+CDMA  S LVAA+                 
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

BLAST of Cmc06g0161441 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 149.4 bits (376), Expect = 3.1e-36
Identity = 107/290 (36.90%), Postives = 123/290 (42.41%), Query Frame = 0

Query: 16  LRSVDPRLYGIIKKEKQFQINGIELFASENYVRRAVMEALGSHLTNKYSKGIPGARYYGG 75
           L  VDP +  II KEK  Q   +EL ASEN+  RAVMEA+GS LTNKYS+G+PG RYYGG
Sbjct: 83  LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 142

Query: 76  NQYIGEIEILCHERAF---------W---------------------------------- 135
           N+YI ++E LC  RA          W                                  
Sbjct: 143 NEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPH 202

Query: 136 ----QHLILILTRG---------------------------ELTALAFCPKLLICGGSAY 195
                H  +   R                            E TA  F PKL+I G SAY
Sbjct: 203 GGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAY 262

Query: 196 PRELDYARFRQIADKFGAVLMCDMAQISGLVAAKVK----EY------------------ 198
            R+ DY R R+IAD  GA LM DMA ISGLVAA V     EY                  
Sbjct: 263 SRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGG 322

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK11173.12.5e-8077.46serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo var. makuwa][more]
KAA0038974.11.4e-7876.06serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo var. makuwa][more]
KAA0038985.11.2e-7774.77serine hydroxymethyltransferase 7-like isoform X1 [Cucumis melo var. makuwa][more]
XP_004146284.11.3e-6852.81serine hydroxymethyltransferase 7 [Cucumis sativus] >KGN57592.1 hypothetical pro... [more]
XP_016901913.11.9e-6752.19PREDICTED: serine hydroxymethyltransferase 6-like isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LM591.2e-5341.61Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
Q84WV02.7e-5342.50Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
O232542.6e-4841.56Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM46.3e-4237.66Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q54Z261.8e-4137.21Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A5D3CH071.2e-8077.46Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7T6546.7e-7976.06Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TC665.6e-7874.77Glycine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A0A0LAD16.2e-6952.81Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G221750 PE=3 ... [more]
A0A1S3C2G69.0e-6852.19Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496109 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G22020.18.6e-5541.61serine hydroxymethyltransferase 6 [more]
AT1G36370.11.9e-5442.50serine hydroxymethyltransferase 7 [more]
AT4G13930.11.9e-4941.56serine hydroxymethyltransferase 4 [more]
AT4G13890.14.5e-4337.66Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G32520.13.1e-3636.90serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 45..91
e-value: 1.9E-27
score: 97.7
coord: 157..199
e-value: 8.0E-12
score: 46.7
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 105..156
e-value: 3.0E-20
score: 73.9
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 20..44
e-value: 1.9E-27
score: 97.7
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 104..156
e-value: 4.1E-18
score: 65.2
coord: 17..91
e-value: 4.4E-27
score: 94.7
coord: 158..198
e-value: 3.9E-11
score: 42.2
NoneNo IPR availablePANTHERPTHR11680:SF7SERINE HYDROXYMETHYLTRANSFERASE 7coord: 103..155
NoneNo IPR availablePANTHERPTHR11680:SF7SERINE HYDROXYMETHYLTRANSFERASE 7coord: 157..197
NoneNo IPR availablePANTHERPTHR11680:SF7SERINE HYDROXYMETHYLTRANSFERASE 7coord: 3..91
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 157..197
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 3..91
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 103..155
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 6..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc06g0161441.1Cmc06g0161441.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity