Cmc02g0037541 (gene) Melon (Charmono) v1.1

Overview
NameCmc02g0037541
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Descriptionnicotianamine synthase-like
LocationCMiso1.1chr02: 329301 .. 331399 (-)
RNA-Seq ExpressionCmc02g0037541
SyntenyCmc02g0037541
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAAAAAACAAGAGTTTCTTTTGAACTCTCTTTTGTATTATTTTTTGTTTTTCTTCTTTATTTTTTGCTCATAGTGTCGACATGGTTTGTGAAGAAGAAACTCTAATCCAAAGGGTTTGTGAATTATATGATGAAATCACAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAGCAAATGAGATCAAATCTCATTAGTCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATTTGTTGGCCAAATTTGATTCCCCTATCGATCATCTTCATGTCTTCCCTTACTTCTCTAATTACCTTAAACTTAGCCTTCTTGAATTCTCTATTCTCCGCCGCCATGGCCCCCGTGTGCTTCCCTCTGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCATCGTCCTTGCCACTCGCCACCTCACCTCCACCGTCTTCCACAACTACGATCTCGACCCGGTTGCTAATTCCAAGGTTGTATGTTGTCTTTGCAAGAAAAATTATATGGGTTTGCTAGTTTGGTTTGTTGCAATAATTTTCCGACTATGTTTGTGAATTTTTTAATTTAGAAGTGATTTAATAAGGTATTAGGAAAATTTTCTTAACCCTCGTATTGATTTTGAGAATCACAAATGATTTTTCTTTATTTTTGAAATATGATATCCATGTTTTAAAGAGTCCTCGAAGTTTTTGATAACTTCTAAGAACAAAAACAGACTTTTTGGAAAAATTGTTTTCTAGACCCTATTAGATAATCATTTGATTTTTTGTTTTTAGTAATTTTCTAAAAAATATTTATGTTTATAAATACTTTTTTTATCTATGTTTTACTCGTATTTTTAAAAATAAATGTCAAGTTTTGAAACTAAAAAGGAAAACCTTTTTTTATTTTATTTTTGAAATTAGGTTAAAAATTTGACTCTAATGCTAAAGATAAATGGAAAACATTTTATATAAATTGAGAGAAAAGAAGGCTAATTTTTCAAAAATTTATCAAATAGAGTGTATTTTTAAAACATGGTTTTTGAAAAGATTACAAAGCATTAAAACTTATGGTTTGAGAACGAAATTAATTAATGTTCATAAGATTAATAATCTGTTAAAAGAAAAACAGATATTTATCAAATGGCAGGTATGGAGTACTAATAAACTCGTATTTTCTTCCTACAGGCGTCGAATTTGGTGTCGCTTGACCCGGACCTGAAGACGAGGATGGTGTTTCACACATGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTACGAGGTTGTGTTTTTGGCAGCCCTTGTAGGAATGGAGAAGGACGAAAAATTGAAGGTGATTAAGCATTTGTCAGATTACATGTCAGAAGGAGCTTATTTGATGGTAAGAAGTGCTCATGGAGGAAGAGCTTTTCTATATCCTGTGGTTGAAGATTCTGATTTGTTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACTGATGAAGTTATCAACTCAGTAATAATTGCAAGAAAAACAACGAAATATGATAACGAAAATGACAGCAACGACAACAACGACGACAACTGTTGTTTGTTTGTTAATTCAGACGAAAATGAGAAGGTGAAGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATTCAAAACGGCTTCAATAATAATCATGGAGGTAAGATTGAGGAATTTGCAATGGAAGCGGCTGAATAATAATAATAACAATTCACCCTACTTGAACAATTCTTGTTAAGTGGGGGTTTCTGATTCTGATTCTCAAAATTATTATGTGTTCCTCTTTGTTTTCAACATGGGAAAATAATTTTGATTTTTCATATTTGAGGAACACATTCATGGGGATTAAGGAGCTAGCTAGCATATATAGCAAAGGTTTTATATATATATATATATATATATATATATATATATATATATATATATGTTTTTTGTTTTTGTTTTTGTTTTTGATTAATGTTGTTGTG

mRNA sequence

GAGAAAAAAAACAAGAGTTTCTTTTGAACTCTCTTTTGTATTATTTTTTGTTTTTCTTCTTTATTTTTTGCTCATAGTGTCGACATGGTTTGTGAAGAAGAAACTCTAATCCAAAGGGTTTGTGAATTATATGATGAAATCACAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAGCAAATGAGATCAAATCTCATTAGTCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATTTGTTGGCCAAATTTGATTCCCCTATCGATCATCTTCATGTCTTCCCTTACTTCTCTAATTACCTTAAACTTAGCCTTCTTGAATTCTCTATTCTCCGCCGCCATGGCCCCCGTGTGCTTCCCTCTGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCATCGTCCTTGCCACTCGCCACCTCACCTCCACCGTCTTCCACAACTACGATCTCGACCCGGTTGCTAATTCCAAGGCGTCGAATTTGGTGTCGCTTGACCCGGACCTGAAGACGAGGATGGTGTTTCACACATGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTACGAGGTTGTGTTTTTGGCAGCCCTTGTAGGAATGGAGAAGGACGAAAAATTGAAGGTGATTAAGCATTTGTCAGATTACATGTCAGAAGGAGCTTATTTGATGGTAAGAAGTGCTCATGGAGGAAGAGCTTTTCTATATCCTGTGGTTGAAGATTCTGATTTGTTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACTGATGAAGTTATCAACTCAGTAATAATTGCAAGAAAAACAACGAAATATGATAACGAAAATGACAGCAACGACAACAACGACGACAACTGTTGTTTGTTTGTTAATTCAGACGAAAATGAGAAGGTGAAGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATTCAAAACGGCTTCAATAATAATCATGGAGGTAAGATTGAGGAATTTGCAATGGAAGCGGCTGAATAATAATAATAACAATTCACCCTACTTGAACAATTCTTGTTAAGTGGGGGTTTCTGATTCTGATTCTCAAAATTATTATGTGTTCCTCTTTGTTTTCAACATGGGAAAATAATTTTGATTTTTCATATTTGAGGAACACATTCATGGGGATTAAGGAGCTAGCTAGCATATATAGCAAAGGTTTTATATATATATATATATATATATATATATATATATATATATATATATGTTTTTTGTTTTTGTTTTTGTTTTTGATTAATGTTGTTGTG

Coding sequence (CDS)

ATGGTTTGTGAAGAAGAAACTCTAATCCAAAGGGTTTGTGAATTATATGATGAAATCACAAGCCTTGAAAGCCTCAAACCTTGTAACAATGTCGACACTCTTTTCACTAAGTTGGTGGTCACGTGCATGTCACCCACCTCCCCTCATTTCCATATCAATTCACTCTCAAAACCACTTCAGCAAATGAGATCAAATCTCATTAGTCTTTGTGGTCAAGCTGAGTCTCTTTTAGAGTCTCATTTTTCTGATTTGTTGGCCAAATTTGATTCCCCTATCGATCATCTTCATGTCTTCCCTTACTTCTCTAATTACCTTAAACTTAGCCTTCTTGAATTCTCTATTCTCCGCCGCCATGGCCCCCGTGTGCTTCCCTCTGCCGTTGCATTCGTTGGCTCTGGCCCTCTTCCTCTTACCTCCATCGTCCTTGCCACTCGCCACCTCACCTCCACCGTCTTCCACAACTACGATCTCGACCCGGTTGCTAATTCCAAGGCGTCGAATTTGGTGTCGCTTGACCCGGACCTGAAGACGAGGATGGTGTTTCACACATGCGACATCATGAAAGTGACAGAGGAGCTAAAACAGTACGAGGTTGTGTTTTTGGCAGCCCTTGTAGGAATGGAGAAGGACGAAAAATTGAAGGTGATTAAGCATTTGTCAGATTACATGTCAGAAGGAGCTTATTTGATGGTAAGAAGTGCTCATGGAGGAAGAGCTTTTCTATATCCTGTGGTTGAAGATTCTGATTTGTTAGGGTTTGAGGTTCTATCTGTGTTTCATCCCACTGATGAAGTTATCAACTCAGTAATAATTGCAAGAAAAACAACGAAATATGATAACGAAAATGACAGCAACGACAACAACGACGACAACTGTTGTTTGTTTGTTAATTCAGACGAAAATGAGAAGGTGAAGATTAGTAGTGGTATTGATCATAAGAAGGATAATAATGGTGTTGTTCATAATAACAAATGTTCTGAGATTCAAAACGGCTTCAATAATAATCATGGAGGTAAGATTGAGGAATTTGCAATGGAAGCGGCTGAATAA

Protein sequence

MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE
Homology
BLAST of Cmc02g0037541 vs. NCBI nr
Match: XP_008450545.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo])

HSP 1 Score: 684.1 bits (1764), Expect = 6.3e-193
Identity = 347/349 (99.43%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQRVCELYDEI+SLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRS+LISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
           RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300
           LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300

Query: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE
Sbjct: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 349

BLAST of Cmc02g0037541 vs. NCBI nr
Match: XP_004135580.1 (nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_007577 [Cucumis sativus])

HSP 1 Score: 620.5 bits (1599), Expect = 8.6e-174
Identity = 321/351 (91.45%), Postives = 331/351 (94.30%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQ+VCELYDEI+SLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPIDHLHVFPYFSNY+KLSLLEFSIL RHGP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
           R LPSAVAFVGSGPLPLTS+VLATRHLTSTVFHNYDLDPVANSKASNLVS DPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS+YMSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDS--NDNNDDNCCLFVNS 300
           LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKT K+DN+NDS  N+NND+ CCLFVNS
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           DENE VKISS         GVVHNNKCSEIQNGF NNHGGKIEEFAMEAAE
Sbjct: 301 DENENVKISS---------GVVHNNKCSEIQNGF-NNHGGKIEEFAMEAAE 341

BLAST of Cmc02g0037541 vs. NCBI nr
Match: KAA0050950.1 (nicotianamine synthase-like [Cucumis melo var. makuwa] >TYK10297.1 nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 563.9 bits (1452), Expect = 9.5e-157
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 62  MRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 121
           MRS+LISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR
Sbjct: 1   MRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 60

Query: 122 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 181
           VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF
Sbjct: 61  VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 120

Query: 182 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 241
           HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL
Sbjct: 121 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 180

Query: 242 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN 301
           YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN
Sbjct: 181 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN 240

Query: 302 EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE
Sbjct: 241 EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 288

BLAST of Cmc02g0037541 vs. NCBI nr
Match: XP_038878580.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 535.0 bits (1377), Expect = 4.7e-148
Identity = 291/371 (78.44%), Postives = 309/371 (83.29%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEE LIQ+V ELYDEI+SLESLKPCN+VDTLFTKLVVTCMSPTSPHFHINSLSK LQ
Sbjct: 1   MVCEEEALIQKVSELYDEISSLESLKPCNHVDTLFTKLVVTCMSPTSPHFHINSLSKSLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPI HLHVFPYFSNYLKLSLLEFSILRRH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIHHLHVFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
            VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLD +ANSKASNLVSLDPDLKTRMV
Sbjct: 121 -VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDLLANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKL+VIKHLS++MSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLRVIKHLSEFMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDN------------- 300
           LYPVVED DL+GFEVLSVFHPTDEVINSVIIA+K   +D+ ND++DN             
Sbjct: 241 LYPVVEDCDLVGFEVLSVFHPTDEVINSVIIAQKKMIFDDNNDNDDNRDYLSVNSDKLEE 300

Query: 301 -------------------------NDDNCCLFVNS-----DENEKVKISSGIDHKKDNN 329
                                    +DD+ CL VNS     DENEKV +S GIDH K+NN
Sbjct: 301 DENEKVMISGFDRKNNGVMHNDKDSDDDDGCLSVNSSKQEKDENEKVMLSGGIDH-KNNN 360

BLAST of Cmc02g0037541 vs. NCBI nr
Match: XP_023529837.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.6e-132
Identity = 255/344 (74.13%), Postives = 291/344 (84.59%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQ+V +LY++I+ LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS P+Q
Sbjct: 1   MVTEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+ HLH+FPYFSNYLKLSLLEFSILRRH P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
            VLPS+VAFVGSGPLPLTSIVLATRHL ST FHNYDLDP+ANS+AS LVS+D DLKTRMV
Sbjct: 121 -VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDADLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEK+EKL+VI+HL ++M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K        D++DD+ CL +  D+
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK--------DHDDDDDCLKIVGDD 300

Query: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFA 345
           N +  I +G+        VVHN +C +  +GF NNHGG IEE A
Sbjct: 301 NHENGIENGV--------VVHNKQCCDHIHGF-NNHGGMIEELA 326

BLAST of Cmc02g0037541 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.2e-91
Identity = 168/294 (57.14%), Postives = 225/294 (76.53%), Query Frame = 0

Query: 3   CEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ ++C+LY++I+ LE+LKPC +VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 122
           RSNLI +CG+AE  LE HFS +L  F D+P+ HL++FPY++NYLKLS LEF +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 182
            +P  VAF+GSGPLPLTS+VLA+ HL  ++FHN+D+DP AN  A+ LVS DPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K EK+KV++HL  +MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDN--ENDSNDNNDDNCC 294
           YP+VE  DL GFEVLSV+HPTDEVINS++I+RK  +  N   +D  D   D  C
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLAC 296

BLAST of Cmc02g0037541 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.5e-91
Identity = 166/275 (60.36%), Postives = 217/275 (78.91%), Query Frame = 0

Query: 1   MVC-EEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPL 60
           MVC     ++++VCELY++I+ LE+L P  +V+ LFT LV TCM P      ++ L + +
Sbjct: 1   MVCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP--IDVSKLCQKI 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHG 120
           Q++RS+LI LCGQAE LLESHFS +L+ +++P+ HLH+FPYF NY+KLSLLE++IL ++ 
Sbjct: 61  QEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNT 120

Query: 121 PRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRM 180
             + P  +AF+GSGPLPLTS+VLAT+HL +T FHNYD+D  AN  AS LV+ DPD+ +RM
Sbjct: 121 TNI-PKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRA 240
            FHT D+M VT  LK Y+VVFLAALVGM+K++K+KV+ HL+ YMS GA LM+RSAHG RA
Sbjct: 181 TFHTADVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARA 240

Query: 241 FLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           FLYPV++  DL GFEVLSV+HPTDEVINSVIIARK
Sbjct: 241 FLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIARK 272

BLAST of Cmc02g0037541 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.6e-88
Identity = 160/275 (58.18%), Postives = 215/275 (78.18%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q +C+LY++I+ LESLKP  +V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRHG 120
           ++R NLI +CG AE  LE+HFS +L  + D+P+ HL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 PRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRM 180
              +P +VAF+GSGPLPLTSIVLA+ HL  T+FHN+D+DP ANS AS LVS DPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM K+EK+KVI+HL  +M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           FLYP+VE  DL GFEVLS++HPTD+VINSV+I++K
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

BLAST of Cmc02g0037541 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.2e-87
Identity = 156/277 (56.32%), Postives = 212/277 (76.53%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+I+ L+SLKP  NVDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF---DSPIDHLHVFPYFSNYLKLSLLEFSILRR 120
            MR+NLI LCG+AE  LE HFS +L       +P+DHLH+FPY+SNYLKL  LEF +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HGPRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKT 180
           H   V P+ +AFVGSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS DPDL  
Sbjct: 121 HSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 180

Query: 181 RMVFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGG 240
           RM+FHT D++  TE L QY+VVFLAALVGM+K+ K+K I+HL  +M+ GA LM+RSAH  
Sbjct: 181 RMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHAL 240

Query: 241 RAFLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           RAFLYP+V+ SDL GF++L+++HPTD+V+NSV+IARK
Sbjct: 241 RAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of Cmc02g0037541 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.9e-85
Identity = 155/276 (56.16%), Postives = 212/276 (76.81%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++I++LESLKP  NVDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRH 120
           + MRS+LI LCG+AE  LE HFS +L  F D+P++HLH+FPY++NYLKL  LEF +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 GPRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTR 180
              V P+ VAF+GSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS D DL  R
Sbjct: 121 TTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGR 240
           M+FHT D++   E L QY+VVFLAALVGM+K+ K+K I+HL  +M+ GA +M+RSAHG R
Sbjct: 181 MIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLR 240

Query: 241 AFLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           AFLYP+V+  DL GFEVL+++HP+D+V+NSV+IARK
Sbjct: 241 AFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of Cmc02g0037541 vs. ExPASy TrEMBL
Match: A0A1S3BPH1 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.1e-193
Identity = 347/349 (99.43%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQRVCELYDEI+SLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRS+LISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
           RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300
           LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300

Query: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE
Sbjct: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 349

BLAST of Cmc02g0037541 vs. ExPASy TrEMBL
Match: A0A0A0LVX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.1e-174
Identity = 321/351 (91.45%), Postives = 331/351 (94.30%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MVCEEETLIQ+VCELYDEI+SLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLE HFSDLLAKFDSPIDHLHVFPYFSNY+KLSLLEFSIL RHGP
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
           R LPSAVAFVGSGPLPLTS+VLATRHLTSTVFHNYDLDPVANSKASNLVS DPDLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELKQYEVVFLAALVGMEK+EKLKVIKHLS+YMSEGAYLMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDS--NDNNDDNCCLFVNS 300
           LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKT K+DN+NDS  N+NND+ CCLFVNS
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DENEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           DENE VKISS         GVVHNNKCSEIQNGF NNHGGKIEEFAMEAAE
Sbjct: 301 DENENVKISS---------GVVHNNKCSEIQNGF-NNHGGKIEEFAMEAAE 341

BLAST of Cmc02g0037541 vs. ExPASy TrEMBL
Match: A0A5A7U6Y1 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004490 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.6e-157
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 62  MRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 121
           MRS+LISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR
Sbjct: 1   MRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 60

Query: 122 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 181
           VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF
Sbjct: 61  VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 120

Query: 182 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 241
           HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL
Sbjct: 121 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 180

Query: 242 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN 301
           YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN
Sbjct: 181 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDEN 240

Query: 302 EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 350
           EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE
Sbjct: 241 EKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFAMEAAE 288

BLAST of Cmc02g0037541 vs. ExPASy TrEMBL
Match: A0A6J1JPC1 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 255/344 (74.13%), Postives = 287/344 (83.43%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQ+V +LY++I+ LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+ HLH+FPYFSNYLKLSLLEFSILRRH P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
            +LPS+VAFVGSGPLPLTSIVLATRHL ST FHNYDLDP+ANS+AS LVS+D DLKTRMV
Sbjct: 121 -ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEK+EKL+VI+HL ++M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K           DD+C   V  D 
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK-----------DDDCLKIVGDDN 300

Query: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFA 345
           +E     +GI    +N  VVHN +C +  +GF NNHGG IEE A
Sbjct: 301 HE-----NGI----ENGVVVHNKQCCDHIHGF-NNHGGMIEELA 322

BLAST of Cmc02g0037541 vs. ExPASy TrEMBL
Match: A0A6J1EU11 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 254/344 (73.84%), Postives = 286/344 (83.14%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           MV EEE LIQ+V +LY++I+ LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120
           QMRSNLI LCGQAESLLESHFSDLLA FD P+ HLH+FPYFSNYLKLSLLEFSILRRH P
Sbjct: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120

Query: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180
            VLPS+VAFVGSGPLPLTSIVLATRHL ST FHNYDLDP+AN++AS LVS+D DLKTRMV
Sbjct: 121 -VLPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANTQASKLVSVDSDLKTRMV 180

Query: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEK+EKL+VI+HL ++M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240

Query: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K        D++DD+C   V  D 
Sbjct: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK--------DHDDDDCLKIVGDDN 300

Query: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFA 345
           +E             N  VVHN +C +  +GF NNHGG IEE A
Sbjct: 301 HE-------------NGVVVHNKQCCDHIHGF-NNHGGMIEELA 321

BLAST of Cmc02g0037541 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 336.3 bits (861), Expect = 3.0e-92
Identity = 168/294 (57.14%), Postives = 225/294 (76.53%), Query Frame = 0

Query: 3   CEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQQM 62
           C+++ L+ ++C+LY++I+ LE+LKPC +VDTLF +LV TC+ P +P+  +  +S+ +Q+M
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCI-PPNPNIDVTKMSENIQEM 63

Query: 63  RSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRHGPR 122
           RSNLI +CG+AE  LE HFS +L  F D+P+ HL++FPY++NYLKLS LEF +L ++   
Sbjct: 64  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 123

Query: 123 VLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMVF 182
            +P  VAF+GSGPLPLTS+VLA+ HL  ++FHN+D+DP AN  A+ LVS DPDL  RM F
Sbjct: 124 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 183

Query: 183 HTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAFL 242
           HT DIM VTE LK ++VVFLAALVGM+K EK+KV++HL  +MS GA LM+RSAHG RAFL
Sbjct: 184 HTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFL 243

Query: 243 YPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDN--ENDSNDNNDDNCC 294
           YP+VE  DL GFEVLSV+HPTDEVINS++I+RK  +  N   +D  D   D  C
Sbjct: 244 YPIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGEDANGVVHDHIDQASDLAC 296

BLAST of Cmc02g0037541 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 327.0 bits (837), Expect = 1.8e-89
Identity = 160/275 (58.18%), Postives = 215/275 (78.18%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C++E L+Q +C+LY++I+ LESLKP  +V+ LF +LV TC+ P +P+  +  +   +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCI-PPNPNIDVTKMCDRVQ 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRHG 120
           ++R NLI +CG AE  LE+HFS +L  + D+P+ HL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 PRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRM 180
              +P +VAF+GSGPLPLTSIVLA+ HL  T+FHN+D+DP ANS AS LVS DPD+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM K+EK+KVI+HL  +M+ GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           FLYP+VE  DL GFEVLS++HPTD+VINSV+I++K
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

BLAST of Cmc02g0037541 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-88
Identity = 156/277 (56.32%), Postives = 212/277 (76.53%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60
           M C+   +++++ +LYD+I+ L+SLKP  NVDTLF +LV TC+ PT  +  + ++ + ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL-PTDTNIDVTNMCEEVK 60

Query: 61  QMRSNLISLCGQAESLLESHFSDLLAKF---DSPIDHLHVFPYFSNYLKLSLLEFSILRR 120
            MR+NLI LCG+AE  LE HFS +L       +P+DHLH+FPY+SNYLKL  LEF +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HGPRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKT 180
           H   V P+ +AFVGSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS DPDL  
Sbjct: 121 HSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSK 180

Query: 181 RMVFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGG 240
           RM+FHT D++  TE L QY+VVFLAALVGM+K+ K+K I+HL  +M+ GA LM+RSAH  
Sbjct: 181 RMIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHAL 240

Query: 241 RAFLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           RAFLYP+V+ SDL GF++L+++HPTD+V+NSV+IARK
Sbjct: 241 RAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of Cmc02g0037541 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 315.8 bits (808), Expect = 4.2e-86
Identity = 155/276 (56.16%), Postives = 212/276 (76.81%), Query Frame = 0

Query: 1   MVCEEETLIQRVCELYDEITSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSL-SKPL 60
           M CE   +++++ +LY++I++LESLKP  NVDTLF +LV TC+ PT  +  +  +  + +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL-PTDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLISLCGQAESLLESHFSDLLAKF-DSPIDHLHVFPYFSNYLKLSLLEFSILRRH 120
           + MRS+LI LCG+AE  LE HFS +L  F D+P++HLH+FPY++NYLKL  LEF +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 GPRVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTR 180
              V P+ VAF+GSGP+PLTSIVLA  HL +T FHN+D+D  AN+ ASNLVS D DL  R
Sbjct: 121 TTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKR 180

Query: 181 MVFHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGR 240
           M+FHT D++   E L QY+VVFLAALVGM+K+ K+K I+HL  +M+ GA +M+RSAHG R
Sbjct: 181 MIFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLR 240

Query: 241 AFLYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARK 275
           AFLYP+V+  DL GFEVL+++HP+D+V+NSV+IARK
Sbjct: 241 AFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of Cmc02g0037541 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-09
Identity = 31/56 (55.36%), Postives = 40/56 (71.43%), Query Frame = 0

Query: 223 MSEGAYLMVRSAHGGRAFLYPVVEDSDLLGFEVLSVFHP--TDEVINSVIIARKTT 277
           M  GA LM+RSA G R+FLY  V+  DL GFEVL ++HP  +D  +NSV++ARK T
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLT 56

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008450545.16.3e-19399.43PREDICTED: nicotianamine synthase-like [Cucumis melo][more]
XP_004135580.18.6e-17491.45nicotianamine synthase [Cucumis sativus] >KGN66010.1 hypothetical protein Csa_00... [more]
KAA0050950.19.5e-15799.65nicotianamine synthase-like [Cucumis melo var. makuwa] >TYK10297.1 nicotianamine... [more]
XP_038878580.14.7e-14878.44probable nicotianamine synthase 4 [Benincasa hispida][more]
XP_023529837.13.6e-13274.13nicotianamine synthase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9C7X54.2e-9157.14Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
Q9XGI75.5e-9160.36Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
O804832.6e-8858.18Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Q9FF792.2e-8756.32Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
Q9FKT95.9e-8556.16Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BPH13.1e-19399.43nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103492113 PE=3 SV=1[more]
A0A0A0LVX14.1e-17491.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G561410 PE=3 SV=1[more]
A0A5A7U6Y14.6e-15799.65Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A6J1JPC11.6e-13074.13nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111486493 PE=3 SV=... [more]
A0A6J1EU111.6e-13073.84nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111437742 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G56430.13.0e-9257.14nicotianamine synthase 4 [more]
AT1G09240.11.8e-8958.18nicotianamine synthase 3 [more]
AT5G04950.11.5e-8856.32nicotianamine synthase 1 [more]
AT5G56080.14.2e-8656.16nicotianamine synthase 2 [more]
AT4G26483.12.4e-0955.36nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 345..349
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 7..276
e-value: 3.7E-60
score: 205.3
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 5..276
e-value: 1.7E-115
score: 385.1
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 4..300
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 1..279
score: 54.874557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc02g0037541.1Cmc02g0037541.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity