CmaCh20G006500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G006500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionLOW QUALITY PROTEIN: uncharacterized protein LOC111449024
LocationCma_Chr20: 3005517 .. 3006739 (-)
RNA-Seq ExpressionCmaCh20G006500
SyntenyCmaCh20G006500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCCGGGATGTTTGGAGAAGATGATAGAAAAGCCATAGAAGTACCCGTTTTCGATTTGGAAACCATACTCAATGCTACAGACAACTTCTCTGAAGCAAACAAAATTGGACGAGGAGGATTTGGGACAGTTTACAAGGTTTGCATAACAGTTTTCCAGCAATTAACTTGGTTTATAAACAACTAACAGTTTCTGGAAATTGCAATAAAAAGACTGTCCCAAGGTTCCGCTCAAGGCTTATATGAGTTTAACAATGAAGCGATTCTCATCGCAAAACTTCAGCATCGAAACCTCGTTAGACTTTTGGGCAATTGTGTTGCAGGAGATGAAAAAATTTTGGTCTATGAATATATGCCCAACAAAAGCTTAGATTTTTTTATATTTGGCACGTCTCTCACCTCTGCCTAATTACCTTGATGATTACACTTGAAGCTAAGCTTCCAAACTCCTTCTCTTTGGTAGATCGAACCCTATGTTTGCTGGTGAATTGGGAGATGCGGTTTAATATCATCTCTGTTTGCCAAGAGAGTTGTTGGAACTTAGTTCAATTGATATCTATCCTTGTAGTCACATTCAAATTGATGTTTCAGTTGTTGTATATTGCAGCGGCTACATGTACCCAGAATATGCGATGGATGGATCATTCTCAGACGTGTTCAGTTTTGGTATAGTTGTGCTGGAGATTGTTAGTGGGATAAGGAACACAGGGTTCTACCAGTCGGTAGAAGCACTGAATCTTCTTGGATATGTCAGAATAAGTCTTCTTCAATAAAGTTGCTTCTATCCAAAACTCCTTTTACTTTAGTTTGAACTGGATGTTTGTTGGGTGGTGGTGTACGCAGGTATGGAAGTTGTGGAGTGGAAAGAGAGCAACGGAAATAGCAGAGGTAGCCATGCGAGAAAGCTACAATCCAAGTGAAGTTGTTAAATGTGTGGCTGTGGGATTGCTGTGTGTACAAGAAGACCCCAGCGATCGCCCCACCATGTCAAATGTGGCCTTTATGCTCAGCTCCGGCAGTGATCCTGCTTCTCTTCCAATTCCTAAACAGCCAGCTTTCCTTGATAAGAGTTCAACTCCTTCTACTTCTTCTACAACAACCTCTTTGGAGTTTATGCAAGAAAATGTAAACGATGATTTTAGCTTACCTGACCCGGTAGTCATCCTATTTCTCGTGGCTTTGCCTTTTGTTTTCACGGTAGGTGTTAAGAATGAGAAATAG

mRNA sequence

ATGGGTTCCGGGATGTTTGGAGAAGATGATAGAAAAGCCATAGAAGTACCCGTTTTCGATTTGGAAACCATACTCAATGCTACAGACAACTTCTCTGAAGCAAACAAAATTGGACGAGGAGGATTTGGGACAGTTTACAAGGTTTGCATAACAGTTTTCCAGCAATTAACTTGCGGCTACATGTACCCAGAATATGCGATGGATGGATCATTCTCAGACGTGTTCAGTTTTGGTATAGTTGTGCTGGAGATTGTTAGTGGGATAAGGAACACAGGGTTCTACCAGTCGGTAGAAGCACTGAATCTTCTTGGATATGTATGGAAGTTGTGGAGTGGAAAGAGAGCAACGGAAATAGCAGAGGTAGCCATGCGAGAAAGCTACAATCCAAGTGAAGTTGTTAAATGTGTGGCTGTGGGATTGCTGTGTGTACAAGAAGACCCCAGCGATCGCCCCACCATGTCAAATGTGGCCTTTATGCTCAGCTCCGGCAGTGATCCTGCTTCTCTTCCAATTCCTAAACAGCCAGCTTTCCTTGATAAGAGTTCAACTCCTTCTACTTCTTCTACAACAACCTCTTTGGAGTTTATGCAAGAAAATGTAAACGATGATTTTAGCTTACCTGACCCGGTAGTCATCCTATTTCTCGTGGCTTTGCCTTTTGTTTTCACGGTAGGTGTTAAGAATGAGAAATAG

Coding sequence (CDS)

ATGGGTTCCGGGATGTTTGGAGAAGATGATAGAAAAGCCATAGAAGTACCCGTTTTCGATTTGGAAACCATACTCAATGCTACAGACAACTTCTCTGAAGCAAACAAAATTGGACGAGGAGGATTTGGGACAGTTTACAAGGTTTGCATAACAGTTTTCCAGCAATTAACTTGCGGCTACATGTACCCAGAATATGCGATGGATGGATCATTCTCAGACGTGTTCAGTTTTGGTATAGTTGTGCTGGAGATTGTTAGTGGGATAAGGAACACAGGGTTCTACCAGTCGGTAGAAGCACTGAATCTTCTTGGATATGTATGGAAGTTGTGGAGTGGAAAGAGAGCAACGGAAATAGCAGAGGTAGCCATGCGAGAAAGCTACAATCCAAGTGAAGTTGTTAAATGTGTGGCTGTGGGATTGCTGTGTGTACAAGAAGACCCCAGCGATCGCCCCACCATGTCAAATGTGGCCTTTATGCTCAGCTCCGGCAGTGATCCTGCTTCTCTTCCAATTCCTAAACAGCCAGCTTTCCTTGATAAGAGTTCAACTCCTTCTACTTCTTCTACAACAACCTCTTTGGAGTTTATGCAAGAAAATGTAAACGATGATTTTAGCTTACCTGACCCGGTAGTCATCCTATTTCTCGTGGCTTTGCCTTTTGTTTTCACGGTAGGTGTTAAGAATGAGAAATAG

Protein sequence

MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYKVCITVFQQLTCGYMYPEYAMDGSFSDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGKRATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPKQPAFLDKSSTPSTSSTTTSLEFMQENVNDDFSLPDPVVILFLVALPFVFTVGVKNEK
Homology
BLAST of CmaCh20G006500 vs. ExPASy Swiss-Prot
Match: Q9ZR08 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 OS=Arabidopsis thaliana OX=3702 GN=At4g03230 PE=3 SV=3)

HSP 1 Score: 150.2 bits (378), Expect = 2.8e-35
Identity = 80/147 (54.42%), Postives = 106/147 (72.11%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYA++G F   SDVFSFG+VV+E +SG RNTGF++  ++L+LLG+ W LW  +
Sbjct: 697 TYGYMSPEYALEGLFSFKSDVFSFGVVVIETISGKRNTGFHEPEKSLSLLGHAWDLWKAE 756

Query: 117 RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
           R  E+ + A++ES      +KC+ VGLLCVQEDP+DRPTMSNV FML S S+ A+LP PK
Sbjct: 757 RGIELLDQALQESCETEGFLKCLNVGLLCVQEDPNDRPTMSNVVFMLGS-SEAATLPTPK 816

Query: 177 QPAF-LDKSSTPSTSSTTTSLEFMQEN 200
           QPAF L +  + S +S++T  E   EN
Sbjct: 817 QPAFVLRRCPSSSKASSSTKPETCSEN 842


HSP 2 Score: 67.4 bits (163), Expect = 2.4e-10
Identity = 30/45 (66.67%), Postives = 36/45 (80.00%), Query Frame = 0

Query: 3   SGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           SG F +DD + I+VP F+LETIL AT NFS ANK+G+GGFG VYK
Sbjct: 504 SGRFKQDDSQGIDVPSFELETILYATSNFSNANKLGQGGFGPVYK 548

BLAST of CmaCh20G006500 vs. ExPASy Swiss-Prot
Match: Q8GYA4 (Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN=CRK10 PE=1 SV=3)

HSP 1 Score: 129.0 bits (323), Expect = 6.8e-29
Identity = 69/155 (44.52%), Postives = 98/155 (63.23%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAM G +   SDV+SFG++VLEI+SG +N+ FYQ+  A +L+ Y W LWS  
Sbjct: 513 TYGYMSPEYAMHGQYSMKSDVYSFGVLVLEIISGKKNSSFYQTDGAHDLVSYAWGLWSNG 572

Query: 117 RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
           R  E+ + A+ E+   +EVV+CV +GLLCVQEDP++RPT+S +  ML+S +   +LP+P+
Sbjct: 573 RPLELVDPAIVENCQRNEVVRCVHIGLLCVQEDPAERPTLSTIVLMLTSNT--VTLPVPR 632

Query: 177 QPAFLDKSSTPSTSSTTTSLEFMQENVNDDFSLPD 209
           QP    +S        T +         DD S+ D
Sbjct: 633 QPGLFFQSRIGKDPLDTDTTSKSLLGSVDDASITD 665


HSP 2 Score: 47.0 bits (110), Expect = 3.4e-04
Identity = 22/42 (52.38%), Postives = 26/42 (61.90%), Query Frame = 0

Query: 6   FGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           F  DD    +    D  TI  ATD+F E+NKIG+GGFG VYK
Sbjct: 323 FAGDDITTADSLQLDYRTIQTATDDFVESNKIGQGGFGEVYK 364

BLAST of CmaCh20G006500 vs. ExPASy Swiss-Prot
Match: O65468 (Cysteine-rich receptor-like protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CRK8 PE=3 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-28
Identity = 69/143 (48.25%), Postives = 97/143 (67.83%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAM G F   SDV+SFG++VLEI+SG +N+ F +S  A +LL + W+LW+ +
Sbjct: 518 TYGYMAPEYAMHGQFSMKSDVYSFGVLVLEIISGRKNSSFDESDGAQDLLTHTWRLWTNR 577

Query: 117 RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
            A ++ +  +  +   SEVV+C+ +GLLCVQEDP+ RPT+S V  ML+S +   +LP+P+
Sbjct: 578 TALDLVDPLIANNCQNSEVVRCIHIGLLCVQEDPAKRPTISTVFMMLTSNT--VTLPVPR 637

Query: 177 QPAFLDKSS-----TPSTSSTTT 192
           QP F  +SS     T S  STTT
Sbjct: 638 QPGFFIQSSPVKDPTDSDQSTTT 658


HSP 2 Score: 46.6 bits (109), Expect = 4.4e-04
Identity = 21/39 (53.85%), Postives = 26/39 (66.67%), Query Frame = 0

Query: 9   DDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           DD    +    D  TI  AT++F+E+NKIGRGGFG VYK
Sbjct: 331 DDMATADSLQLDYRTIQTATNDFAESNKIGRGGFGEVYK 369

BLAST of CmaCh20G006500 vs. ExPASy Swiss-Prot
Match: Q9LPZ3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g11410 OS=Arabidopsis thaliana OX=3702 GN=At1g11410 PE=3 SV=3)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-28
Identity = 67/125 (53.60%), Postives = 88/125 (70.40%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAMDG F   SDV+SFG+++LEI++G RN+ FY+  E+LNL+ ++W  W   
Sbjct: 688 TYGYMSPEYAMDGQFSIKSDVYSFGVLILEIITGKRNSAFYE--ESLNLVKHIWDRWENG 747

Query: 117 RATEIAEVAM-RESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIP 176
            A EI +  M  E+Y+  EV+KC+ +GLLCVQE+ SDRP MS+V FML  G +   LP P
Sbjct: 748 EAIEIIDKLMGEETYDEGEVMKCLHIGLLCVQENSSDRPDMSSVVFML--GHNAIDLPSP 807

Query: 177 KQPAF 178
           K PAF
Sbjct: 808 KHPAF 808


HSP 2 Score: 52.0 bits (123), Expect = 1.0e-05
Identity = 22/40 (55.00%), Postives = 30/40 (75.00%), Query Frame = 0

Query: 8   EDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           ED  ++ E+P+F+L TI  AT+NF+  NK+G GGFG VYK
Sbjct: 500 EDKSRSRELPLFELSTIATATNNFAFQNKLGAGGFGPVYK 539

BLAST of CmaCh20G006500 vs. ExPASy Swiss-Prot
Match: Q9C5S9 (Cysteine-rich receptor-like protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.4e-28
Identity = 69/159 (43.40%), Postives = 102/159 (64.15%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAM G F   SDV+SFG++VLEI+SG +N+ F +S  A +LL + W+LW+ K
Sbjct: 516 TYGYMAPEYAMHGQFSMKSDVYSFGVLVLEIISGRKNSSFGESDGAQDLLTHAWRLWTNK 575

Query: 117 RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
           +A ++ +  + E+   SEVV+C+ +GLLCVQEDP+ RP +S V  ML+S +   +LP+P+
Sbjct: 576 KALDLVDPLIAENCQNSEVVRCIHIGLLCVQEDPAKRPAISTVFMMLTSNT--VTLPVPR 635

Query: 177 QPAF----------LDKSSTPSTSSTTTSLEFMQENVND 203
           QP F          LD   + +T S   S++   E++ D
Sbjct: 636 QPGFFIQCRAVKDPLDSDQSTTTKSFPASID--DESITD 670


HSP 2 Score: 46.6 bits (109), Expect = 4.4e-04
Identity = 21/39 (53.85%), Postives = 26/39 (66.67%), Query Frame = 0

Query: 9   DDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           DD    +    D  TI  AT++F+E+NKIGRGGFG VYK
Sbjct: 329 DDMATADSLQLDYRTIQTATNDFAESNKIGRGGFGEVYK 367

BLAST of CmaCh20G006500 vs. ExPASy TrEMBL
Match: A0A6J1FX09 (LOW QUALITY PROTEIN: uncharacterized protein LOC111449024 OS=Cucurbita moschata OX=3662 GN=LOC111449024 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 3.2e-82
Identity = 192/352 (54.55%), Postives = 200/352 (56.82%), Query Frame = 0

Query: 1   MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK------------- 60
           MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK             
Sbjct: 642 MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEIAIKR 701

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 702 LSQGSAQGLDEFKNEAILIAKLQHRNLVRLLGYCVAGDEKILVYEYMPNKSLDFFIFDRT 761

Query: 121 VCITVFQQL--------------------------------------------------- 180
           +C+ V  ++                                                   
Sbjct: 762 LCLLVNWEMRFNIIIGIARGLVYLHEDSRLRIIHRHMKTSNVLLDAEMNPKISDFGLARI 821

Query: 181 ---------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVE 211
                          T GYM PEYAMDGSF   SDVFSFGIVVLEIVSGIRNTGFYQS E
Sbjct: 822 FDGKQTEAFTKRVVGTYGYMSPEYAMDGSFSVKSDVFSFGIVVLEIVSGIRNTGFYQSKE 881

BLAST of CmaCh20G006500 vs. ExPASy TrEMBL
Match: A0A6J1JFY2 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484057 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.2e-65
Identity = 164/350 (46.86%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 2    GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
            GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 658  GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 717

Query: 62   -------------------------------VCITVFQQL-------------------- 121
                                            C+   ++L                    
Sbjct: 718  SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 777

Query: 122  ------------------------------------------------------------ 181
                                                                        
Sbjct: 778  CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 837

Query: 182  --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 210
                          T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 838  DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 897

BLAST of CmaCh20G006500 vs. ExPASy TrEMBL
Match: A0A6J1JFJ0 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X6 OS=Cucurbita maxima OX=3661 GN=LOC111484057 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.2e-65
Identity = 164/350 (46.86%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 2    GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
            GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 653  GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 712

Query: 62   -------------------------------VCITVFQQL-------------------- 121
                                            C+   ++L                    
Sbjct: 713  SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 772

Query: 122  ------------------------------------------------------------ 181
                                                                        
Sbjct: 773  CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 832

Query: 182  --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 210
                          T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 833  DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 892

BLAST of CmaCh20G006500 vs. ExPASy TrEMBL
Match: A0A6J1J713 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111484057 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.3e-62
Identity = 159/339 (46.90%), Postives = 168/339 (49.56%), Query Frame = 0

Query: 2   GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
           GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 658 GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 717

Query: 62  -------------------------------VCITVFQQL-------------------- 121
                                           C+   ++L                    
Sbjct: 718 SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 777

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 778 CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 837

Query: 182 --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 199
                         T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 838 DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 897

BLAST of CmaCh20G006500 vs. ExPASy TrEMBL
Match: A0A6J1JFY9 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111484057 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.3e-62
Identity = 159/339 (46.90%), Postives = 168/339 (49.56%), Query Frame = 0

Query: 2   GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
           GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 653 GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 712

Query: 62  -------------------------------VCITVFQQL-------------------- 121
                                           C+   ++L                    
Sbjct: 713 SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 772

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 773 CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 832

Query: 182 --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 199
                         T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 833 DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 892

BLAST of CmaCh20G006500 vs. NCBI nr
Match: XP_022944619.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111449024 [Cucurbita moschata])

HSP 1 Score: 314.7 bits (805), Expect = 6.6e-82
Identity = 192/352 (54.55%), Postives = 200/352 (56.82%), Query Frame = 0

Query: 1   MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK------------- 60
           MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK             
Sbjct: 642 MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEIAIKR 701

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 702 LSQGSAQGLDEFKNEAILIAKLQHRNLVRLLGYCVAGDEKILVYEYMPNKSLDFFIFDRT 761

Query: 121 VCITVFQQL--------------------------------------------------- 180
           +C+ V  ++                                                   
Sbjct: 762 LCLLVNWEMRFNIIIGIARGLVYLHEDSRLRIIHRHMKTSNVLLDAEMNPKISDFGLARI 821

Query: 181 ---------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVE 211
                          T GYM PEYAMDGSF   SDVFSFGIVVLEIVSGIRNTGFYQS E
Sbjct: 822 FDGKQTEAFTKRVVGTYGYMSPEYAMDGSFSVKSDVFSFGIVVLEIVSGIRNTGFYQSKE 881

BLAST of CmaCh20G006500 vs. NCBI nr
Match: XP_023512255.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111777045 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312.8 bits (800), Expect = 2.5e-81
Identity = 190/352 (53.98%), Postives = 197/352 (55.97%), Query Frame = 0

Query: 1   MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK------------- 60
           MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK             
Sbjct: 642 MGSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEIAIKR 701

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 702 LSQGSAQGLDEFKNEAILIAKLQHRNLVRLLGYCVAGDEKILVYEYMPNKSLDFFIFDRT 761

Query: 121 VCITVFQQL--------------------------------------------------- 180
           +C+ V  ++                                                   
Sbjct: 762 LCLLVNWEMRFNIIIGIARGLVYLHEDSRLRIIHRDMKTSNVLLDAEMNPKISDFGLARI 821

Query: 181 ---------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVE 211
                          T  YM PEY MDGSF   SDVFSFGIVVLEIVSGIRNTGFYQS E
Sbjct: 822 FDGKQTEAFTKRVVGTYSYMSPEYTMDGSFSVKSDVFSFGIVVLEIVSGIRNTGFYQSKE 881

BLAST of CmaCh20G006500 vs. NCBI nr
Match: XP_022986238.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X2 [Cucurbita maxima] >XP_022986239.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X2 [Cucurbita maxima])

HSP 1 Score: 259.6 bits (662), Expect = 2.5e-65
Identity = 164/350 (46.86%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 2    GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
            GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 658  GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 717

Query: 62   -------------------------------VCITVFQQL-------------------- 121
                                            C+   ++L                    
Sbjct: 718  SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 777

Query: 122  ------------------------------------------------------------ 181
                                                                        
Sbjct: 778  CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 837

Query: 182  --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 210
                          T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 838  DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 897

BLAST of CmaCh20G006500 vs. NCBI nr
Match: XP_022986244.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X6 [Cucurbita maxima])

HSP 1 Score: 259.6 bits (662), Expect = 2.5e-65
Identity = 164/350 (46.86%), Postives = 177/350 (50.57%), Query Frame = 0

Query: 2    GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
            GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 653  GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 712

Query: 62   -------------------------------VCITVFQQL-------------------- 121
                                            C+   ++L                    
Sbjct: 713  SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 772

Query: 122  ------------------------------------------------------------ 181
                                                                        
Sbjct: 773  CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 832

Query: 182  --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 210
                          T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 833  DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 892

BLAST of CmaCh20G006500 vs. NCBI nr
Match: XP_022986241.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform X3 [Cucurbita maxima])

HSP 1 Score: 249.6 bits (636), Expect = 2.6e-62
Identity = 159/339 (46.90%), Postives = 168/339 (49.56%), Query Frame = 0

Query: 2   GSGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK-------------- 61
           GSGMFGEDDRKAIEVP+  LETILNATDNFSEANKIGRGGFGTVYK              
Sbjct: 658 GSGMFGEDDRKAIEVPILGLETILNATDNFSEANKIGRGGFGTVYKGLFPGGLEVAIKRL 717

Query: 62  -------------------------------VCITVFQQL-------------------- 121
                                           C+   ++L                    
Sbjct: 718 SQGSAQGMDEFKNEAILIAKLQHRNLVRLLGYCVAGVEKLLIYEYMPNKSLDFFIFDRTQ 777

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 778 CLLMNWKMRFNIIMGIARGLVYLHEDSRLRIIHRDMKTSNILLDAEMNPKISDFGLARIF 837

Query: 182 --------------TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEA 199
                         T GYM PEYA+DGSF   SDVFSFGIVVLEIVSG +NTGFYQS EA
Sbjct: 838 DGNQIEGITNRVVGTFGYMPPEYALDGSFSVKSDVFSFGIVVLEIVSGRKNTGFYQSNEA 897

BLAST of CmaCh20G006500 vs. TAIR 10
Match: AT4G03230.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-36
Identity = 80/147 (54.42%), Postives = 106/147 (72.11%), Query Frame = 0

Query: 57   TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
            T GYM PEYA++G F   SDVFSFG+VV+E +SG RNTGF++  ++L+LLG+ W LW  +
Sbjct: 855  TYGYMSPEYALEGLFSFKSDVFSFGVVVIETISGKRNTGFHEPEKSLSLLGHAWDLWKAE 914

Query: 117  RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
            R  E+ + A++ES      +KC+ VGLLCVQEDP+DRPTMSNV FML S S+ A+LP PK
Sbjct: 915  RGIELLDQALQESCETEGFLKCLNVGLLCVQEDPNDRPTMSNVVFMLGS-SEAATLPTPK 974

Query: 177  QPAF-LDKSSTPSTSSTTTSLEFMQEN 200
            QPAF L +  + S +S++T  E   EN
Sbjct: 975  QPAFVLRRCPSSSKASSSTKPETCSEN 1000


HSP 2 Score: 67.4 bits (163), Expect = 1.7e-11
Identity = 30/45 (66.67%), Postives = 36/45 (80.00%), Query Frame = 0

Query: 3   SGMFGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           SG F +DD + I+VP F+LETIL AT NFS ANK+G+GGFG VYK
Sbjct: 662 SGRFKQDDSQGIDVPSFELETILYATSNFSNANKLGQGGFGPVYK 706

BLAST of CmaCh20G006500 vs. TAIR 10
Match: AT4G23180.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 10 )

HSP 1 Score: 129.0 bits (323), Expect = 4.8e-30
Identity = 69/155 (44.52%), Postives = 98/155 (63.23%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAM G +   SDV+SFG++VLEI+SG +N+ FYQ+  A +L+ Y W LWS  
Sbjct: 513 TYGYMSPEYAMHGQYSMKSDVYSFGVLVLEIISGKKNSSFYQTDGAHDLVSYAWGLWSNG 572

Query: 117 RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
           R  E+ + A+ E+   +EVV+CV +GLLCVQEDP++RPT+S +  ML+S +   +LP+P+
Sbjct: 573 RPLELVDPAIVENCQRNEVVRCVHIGLLCVQEDPAERPTLSTIVLMLTSNT--VTLPVPR 632

Query: 177 QPAFLDKSSTPSTSSTTTSLEFMQENVNDDFSLPD 209
           QP    +S        T +         DD S+ D
Sbjct: 633 QPGLFFQSRIGKDPLDTDTTSKSLLGSVDDASITD 665


HSP 2 Score: 47.0 bits (110), Expect = 2.4e-05
Identity = 22/42 (52.38%), Postives = 26/42 (61.90%), Query Frame = 0

Query: 6   FGEDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           F  DD    +    D  TI  ATD+F E+NKIG+GGFG VYK
Sbjct: 323 FAGDDITTADSLQLDYRTIQTATDDFVESNKIGQGGFGEVYK 364

BLAST of CmaCh20G006500 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 128.3 bits (321), Expect = 8.2e-30
Identity = 69/143 (48.25%), Postives = 97/143 (67.83%), Query Frame = 0

Query: 57   TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
            T GYM PEYAM G F   SDV+SFG++VLEI+SG +N+ F +S  A +LL + W+LW+ +
Sbjct: 1104 TYGYMAPEYAMHGQFSMKSDVYSFGVLVLEIISGRKNSSFDESDGAQDLLTHTWRLWTNR 1163

Query: 117  RATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIPK 176
             A ++ +  +  +   SEVV+C+ +GLLCVQEDP+ RPT+S V  ML+S +   +LP+P+
Sbjct: 1164 TALDLVDPLIANNCQNSEVVRCIHIGLLCVQEDPAKRPTISTVFMMLTSNT--VTLPVPR 1223

Query: 177  QPAFLDKSS-----TPSTSSTTT 192
            QP F  +SS     T S  STTT
Sbjct: 1224 QPGFFIQSSPVKDPTDSDQSTTT 1244


HSP 2 Score: 46.6 bits (109), Expect = 3.1e-05
Identity = 21/39 (53.85%), Postives = 26/39 (66.67%), Query Frame = 0

Query: 9   DDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           DD    +    D  TI  AT++F+E+NKIGRGGFG VYK
Sbjct: 917 DDMATADSLQLDYRTIQTATNDFAESNKIGRGGFGEVYK 955

BLAST of CmaCh20G006500 vs. TAIR 10
Match: AT1G11410.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-29
Identity = 67/125 (53.60%), Postives = 88/125 (70.40%), Query Frame = 0

Query: 57  TCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVWKLWSGK 116
           T GYM PEYAMDG F   SDV+SFG+++LEI++G RN+ FY+  E+LNL+ ++W  W   
Sbjct: 688 TYGYMSPEYAMDGQFSIKSDVYSFGVLILEIITGKRNSAFYE--ESLNLVKHIWDRWENG 747

Query: 117 RATEIAEVAM-RESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPASLPIP 176
            A EI +  M  E+Y+  EV+KC+ +GLLCVQE+ SDRP MS+V FML  G +   LP P
Sbjct: 748 EAIEIIDKLMGEETYDEGEVMKCLHIGLLCVQENSSDRPDMSSVVFML--GHNAIDLPSP 807

Query: 177 KQPAF 178
           K PAF
Sbjct: 808 KHPAF 808


HSP 2 Score: 52.0 bits (123), Expect = 7.5e-07
Identity = 22/40 (55.00%), Postives = 30/40 (75.00%), Query Frame = 0

Query: 8   EDDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           ED  ++ E+P+F+L TI  AT+NF+  NK+G GGFG VYK
Sbjct: 500 EDKSRSRELPLFELSTIATATNNFAFQNKLGAGGFGPVYK 539

BLAST of CmaCh20G006500 vs. TAIR 10
Match: AT4G23140.2 (cysteine-rich RLK (RECEPTOR-like protein kinase) 6 )

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 70/165 (42.42%), Postives = 104/165 (63.03%), Query Frame = 0

Query: 51  TVFQQLTCGYMYPEYAMDGSF---SDVFSFGIVVLEIVSGIRNTGFYQSVEALNLLGYVW 110
           T F   + GYM PEYAM G F   SDV+SFG++VLEI+SG +N+ F +S  A +LL + W
Sbjct: 516 TYFVVDSSGYMAPEYAMHGQFSMKSDVYSFGVLVLEIISGRKNSSFGESDGAQDLLTHAW 575

Query: 111 KLWSGKRATEIAEVAMRESYNPSEVVKCVAVGLLCVQEDPSDRPTMSNVAFMLSSGSDPA 170
           +LW+ K+A ++ +  + E+   SEVV+C+ +GLLCVQEDP+ RP +S V  ML+S +   
Sbjct: 576 RLWTNKKALDLVDPLIAENCQNSEVVRCIHIGLLCVQEDPAKRPAISTVFMMLTSNT--V 635

Query: 171 SLPIPKQPAF----------LDKSSTPSTSSTTTSLEFMQENVND 203
           +LP+P+QP F          LD   + +T S   S++   E++ D
Sbjct: 636 TLPVPRQPGFFIQCRAVKDPLDSDQSTTTKSFPASID--DESITD 676


HSP 2 Score: 46.6 bits (109), Expect = 3.1e-05
Identity = 21/39 (53.85%), Postives = 26/39 (66.67%), Query Frame = 0

Query: 9   DDRKAIEVPVFDLETILNATDNFSEANKIGRGGFGTVYK 48
           DD    +    D  TI  AT++F+E+NKIGRGGFG VYK
Sbjct: 329 DDMATADSLQLDYRTIQTATNDFAESNKIGRGGFGEVYK 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZR082.8e-3554.42G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 OS=Arabi... [more]
Q8GYA46.8e-2944.52Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN... [more]
O654681.2e-2848.25Cysteine-rich receptor-like protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LPZ31.5e-2853.60G-type lectin S-receptor-like serine/threonine-protein kinase At1g11410 OS=Arabi... [more]
Q9C5S93.4e-2843.40Cysteine-rich receptor-like protein kinase 6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1FX093.2e-8254.55LOW QUALITY PROTEIN: uncharacterized protein LOC111449024 OS=Cucurbita moschata ... [more]
A0A6J1JFY21.2e-6546.86G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
A0A6J1JFJ01.2e-6546.86G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
A0A6J1J7131.3e-6246.90G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
A0A6J1JFY91.3e-6246.90G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
Match NameE-valueIdentityDescription
XP_022944619.16.6e-8254.55LOW QUALITY PROTEIN: uncharacterized protein LOC111449024 [Cucurbita moschata][more]
XP_023512255.12.5e-8153.98LOW QUALITY PROTEIN: uncharacterized protein LOC111777045 [Cucurbita pepo subsp.... [more]
XP_022986238.12.5e-6546.86G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
XP_022986244.12.5e-6546.86G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
XP_022986241.12.6e-6246.90G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230 isoform ... [more]
Match NameE-valueIdentityDescription
AT4G03230.12.0e-3654.42S-locus lectin protein kinase family protein [more]
AT4G23180.14.8e-3044.52cysteine-rich RLK (RECEPTOR-like protein kinase) 10 [more]
AT4G23160.18.2e-3048.25cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
AT1G11410.11.1e-2953.60S-locus lectin protein kinase family protein [more]
AT4G23140.21.8e-2942.42cysteine-rich RLK (RECEPTOR-like protein kinase) 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 55..161
e-value: 4.4E-12
score: 47.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 6..54
e-value: 4.7E-9
score: 38.2
NoneNo IPR availablePANTHERPTHR27002RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8coord: 15..47
coord: 57..189
NoneNo IPR availablePANTHERPTHR27002:SF442S-LOCUS LECTIN KINASE FAMILY PROTEINcoord: 15..47
coord: 57..189
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 56..157
e-value: 2.9E-5
score: 23.5
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 55..188
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 16..51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G006500.1CmaCh20G006500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity