CmaCh20G005500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh20G005500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionhistone deacetylase HDT1-like
LocationCma_Chr20: 2669602 .. 2674285 (-)
RNA-Seq ExpressionCmaCh20G005500
SyntenyCmaCh20G005500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCCTTTGATTTGCTACTCTTCGTCTTCCTGATATTGTTTTGAACTGTGTTACAGTTAACCTCTACTCCTAAATACTTCTAAAACCCTAAACTCTTCTTCCGCCACTGTCAATGGAGTTCTGGGGTAAGTTCTTTCCATCTTTTCCTTATGCTATTGTCTTTTACTTGTTTTCATAATCGTAATATCGCTTGGCTTTCGCTTTGAAGCTTCTTTCCTCTCGTTTTCTTTTTTAGCTCGGCGGGGGCGGGTACAAACTAGGGTACCCTCTTGAATTTAGTTTTGATGTCTTGAGGAGCATAAACTTAGAGTTTTTGAGTGATGTCTTCAGATGATAATGTTTTATTATCATATTTGATAAATACTCGAAGTTCATAATGTTTTTTTTGGGGGTTTTAGGTGTTGAAGTTAAGTCTGGTCAGGCACTCTCTGTGCAGCCTGGAGATCAGAAGTATCTGCATCTATCACAGGTACTGATGTTACTGCTGTAGCTATTTTTATTTATTTTCCCAGTTGAATCACCGACCCTTCCAATGCAGAGAAATTGATGAAGTATTTTTATGCAGGCGACTCTAGGTGAACTAAAGAAGGATAAAGCGAATGAATCCGTGACAATTTTTGTGAAGATTGGTGACCAGAAGCTTGTTCTGGGAATTCTCTCTGCTGAGAAGTTCCCTCAGCTATCATTTGATCTTGTGTTTGAAAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAAGTATTTACTGTTTGGGATACCAGGCTTCTGCTGAGGACCAAGAACAATATCCTTACCTTTTAATTTGAGGATTTATATTTCCTGCGTTGTTTGGGTTTAATGATCAAGTCAAGCAGCATGCTTTCGTATTTTGGAGCTGCCTGAACATAATATTTTATACGATCCCATTGGTTTTTAGTATTTTTCGATTTTTCATCTATGTATAGTATCCTTCTTTTCCTTAACTTTTATCTCCTGTCTTCCGTGTTCCACTGAAGAGTATTCTGGTAAGTTTTTAAACATTCATAAATGTAGTTTGCTGATTGATCTTTTCATATTCTAATTTTACTATCTGAAATTGATGAGCTGAGTTTGTGTGATCCCATTTCAGATTCTGATTTTGGCTCAGAAGATGAGGAACTTACATTGCAGCCTGTTCAGAATGGTAAATGCAATTGATTGTGTAAAGAAATAGTGTATTAGTAGTAGAAAATATTCATGGCATTGTGCTTAATATCTAGTTAAAATTTACTTTCAGGAAAACCTAGTCAGGAGGAGGAGAAGTCTATTGCGGGAAAAAATTCAAAGAAAGCAGAAGTTAAGTCTCTTGTGCCGAGCAAGGAGGAAGGGGATGATGGCTCTGAAGATGATGATGACTCTGATGAGGACGAGGATTCTGATGATGACTCTGATGAGGTAGTTTAAATGAGACTCTCCCACTTCTAGAACATCTGGTTGCGGTGATTTTAAAAAGGTAGTGCTTTTCTGAGAAGCATGACCTTAGGTGCCTTGGGAGAGTTAATGCATGTGTACGAAGTTTGATTTTTACTGCGAAGTCCCAATTTTATGATTAGGTTATTTCATAGCTGGTGCCTTTTTGAAAAGCGCCCCTTGGTTTTCTTTTCAAAACGCAGCTCTGAGGAAATAGAAAGTCGGTGGACTCCTATAATTTTATTCATTTAGATGGTCGTTAGAGTCTATTAGTGAAGGCGTTATCCTCGTTGAATCCTTTGTTATAGGTTGCCAAGGATTTTGTTAGATGATGGAAGTTCCTTATATGTGTTAATGGTGCTATCGATAAATTGCAGGAAATGCTTGGAGCTGACAGTGACTCTGACGATGAAGATGATGGTACTGAAAGTGAGGAGGAGACACCAAAGAAGGTGTGTTTCAATCCTAGTTCAACTTCTATTTGTAGCAGTTTATTTGAAAATTTGACAAACCCATGTTTATTCTGGTACATTTTTTCTTCTTTTAGCATCATTTTTATCATTTGGTGTCCTCTTGTAGGTTGAATCAGCCAAGAAGAGGCCAAACGAGTCTGCCTCGAAGACTCCTGTTTCCAAGAAGGCAAAAGTAGCTTCGGCTGAGAAGACAGGTGACTCTATCAACCATTCATTTATTTCATGATAACATTAATGAGCATTATTTCATACTTTCGGGTTTAAATACTACTTTGGTCAGTGTATTTTAGAATTTAGTTCATTTTAGTGTCAGTATGTTTAGAATGTTCATTGTGATCCATGTATTCTTAACTTTTGTTCTTTTTGGTCTTTGCATACTCATTGGCTATCATTTTCATTTCATTTTCTTCTGATCAAAATTTTAGGCATGACATTAAATTCACTTAACTTCTTAGGATGTAGACATAAATTTGTATTTTTGTGCATTAAATTCACTAATGATGATCAAAATGGCTACTTTGTAAAAGTGACTAAAATGAACAAAATAATATTTATACCCAATTTTAACATGCTGTTATGTATTGAGCGTTGAATGTATCTAATCATCTCTAGAGTGGCGTGTTCTGTTAGCTTATCTCTTTGTTTATACACTGAACACACAATGTTAGCCTAGCCTAGCTTCGGAAACCTTTGTAGCCTATAAACTTTGTCCCGCCACGCCATTGCTCCTCCATTGAATCAATTTGGTATTGAACATGTCTAGCAGGCTCGAAGAAGGGGGGCCATACAGATACTCCCCACCCTGCCAAGAAACCCGGGAAAACTCCTTCCAAGGGAGAAACCCCAAAATCCGCAGGCCAGGTCTCTTGCAAATCCTGTGACAGGTATGCTGTATGTCCTTATGAATGACTTGCGCTATCAATAGAAGAGTCCTGAGGTTGCTGACGTTACTACCTCTGCTGTTTTTTAGGTCATTTGGTTCCGACAGTGCTCTTCAGTCGCACAGTAAAGCCAAGCATGGCGGTAAGTGAAAGGAAGACGAAGACGGAGAGGAAGTTAGAAACAACAACATATTATTATGCAGCTCCAATATGTAATTCTTCTCTAGATACAGAGGTTGCTATCTTGGCTGAGATTTTTGTGTCGAGTGTGTTTGGATCATACAACGAGAAGCGATTGTTCGAACAAATTGAGGGAGTTTAATTTAGTTTTATTATTGATGTACGTTTTGGGTCTATTTATTAGTGTTTGTAGTTCACGTTTTCTCCTTAGAGTATGTTATCATAGGTTGCCGTTCCATTCTCTGTTTTAGGACGAAAACATCATTATTTTGGAACCCTTATATTGTGTTTTTGTTCCTTTTTTCAAGCTAATGAAAGCCGTAATTTTTGTGCATCAAATTGTCACAGCTCAGCTGGGTGCGATTCATTCTTTTCTAGACGAAAAAATATTAAGATTATGCTCGAATGATCGATGCAATTCATGAAAAACATATTCAATATTGAAACGATAGTTGTTTCATAAACATTTAGTTTCGTGTAGTGGTTTAACTTAAAATGTTTGGATGATCAAACCTCCATAACTCTTCATATTTGCACTAAAAAAAATTAAGGAAATCCAAGAGCCTGTTTTTAATGTAATATTTGTGAAAATATGGCAGAAAATTCGTATATGGGCCTAAGGAATTGGGCTTTGGGTCTGAGCTGAGCCCAAACCATGAACGCCTGGGCCTCCAAGTCCAGCCCAGGCCCACACTGACATCCGCTCGATTATTTGAAACCCTAGGCCTCCTAAAACCTGGCGGCAATTCCCTTATAAAATGCTTTAAACATTTGCCACCACCAATCCGATCGTTCCTTCTCGAGTTTTGTCACGCTTCATTTACTCTTCAGGGCTTTTCTTAAACCCTAGCGTCTTCTACCTACTCGTCAATGGAGTTTTGGGGTAAGCTATTCTGGTTTTTCGTTTCTTTTAGCTAAATCATGTTAGTTTTCTCATTAGAAACACTCGCTGTTATTCAGGTCTTCTCCATGCTTCACTTTTGATCCTGTCGATGTCTGTTTTGTTTTCAGATTGCATTGTATATTGCTCGTTTTAACTTTTTTGTTCTCCCAATTAGTTTATCCCGTTTTCGTTGCATTATATGTGTGAGTAGTAACCTTTTGTTGTATGCCATGCTTTGCTTTCTCCCCTACTAAGCATCTTCTCCTCCTATTCCTTTTTCAAATTTCTTTTTGTGGGGGTGGTGGTCGCTGTCTGTAAATAATCTGTTTCGTAATCTTTAGATTTCCTATAGCCGTAACGAGTTATTTAATCTGTGAGTCGATGGATTGAGAACATTCTTCATGTTTTTGGGATTGTAGGTGTTGAAGTCAAGTCTGGTAAGCCACTTGTTGTGAAGCCTGGAATTCAAAAATATCTGCATCTGTCACAGGTGGGTGTCATTTTTGTTATTTTATTTTAAATTTCCCGTTGAGCTTATGAGTTTCCTGTTTTAGTTACAACTCTACCCATGTTGATAATTTTATGAAACGCTTCTATGCAGGCGACTCTAGGTGAAATAAAGAAAGACAAAGCGAATGAAAATGTGACAATTTTCTTGAAGATTGGTGGCCAGAAGCTTGTTCTGGGAATTCTCTCCGCAGAGAAGTTTCCCCAGCTATCATTTGATCTCGTGTTTGAGAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAAGTGTTTACTGTGCTGGATACCAGGCTTTCGCGGAGGATCAAGAACAATATCCTTTCTTTTGA

mRNA sequence

TGCCTTTGATTTGCTACTCTTCGTCTTCCTGATATTGTTTTGAACTGTGTTACAGTTAACCTCTACTCCTAAATACTTCTAAAACCCTAAACTCTTCTTCCGCCACTGTCAATGGAGTTCTGGGGTGTTGAAGTTAAGTCTGGTCAGGCACTCTCTGTGCAGCCTGGAGATCAGAAGTATCTGCATCTATCACAGGCGACTCTAGGTGAACTAAAGAAGGATAAAGCGAATGAATCCGTGACAATTTTTGTGAAGATTGGTGACCAGAAGCTTGTTCTGGGAATTCTCTCTGCTGAGAAGTTCCCTCAGCTATCATTTGATCTTGTGTTTGAAAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAATATCCTTCTTTTCCTTAACTTTTATCTCCTGTCTTCCGTGTTCCACTGAAGAGTATTCTGATTCTGATTTTGGCTCAGAAGATGAGGAACTTACATTGCAGCCTGTTCAGAATGGAAAACCTAGTCAGGAGGAGGAGAAGTCTATTGCGGGAAAAAATTCAAAGAAAGCAGAAGTTAAGTCTCTTGTGCCGAGCAAGGAGGAAGGGGATGATGGCTCTGAAGATGATGATGACTCTGATGAGGACGAGGATTCTGATGATGACTCTGATGAGGAAATGCTTGGAGCTGACAGTGACTCTGACGATGAAGATGATGGTACTGAAAGTGAGGAGGAGACACCAAAGAAGGTTGAATCAGCCAAGAAGAGGCCAAACGAGTCTGCCTCGAAGACTCCTGTTTCCAAGAAGGCAAAAGTAGCTTCGGCTGAGAAGACAGGCTCGAAGAAGGGGGGCCATACAGATACTCCCCACCCTGCCAAGAAACCCGGGAAAACTCCTTCCAAGGGAGAAACCCCAAAATCCGCAGGCCAGGTCTCTTGCAAATCCTGTGACAGGTCATTTGGTTCCGACAGTGCTCTTCAGTCGCACAGTAAAGCCAAGCATGGCGGTGTTGAAGTCAAGTCTGGTAAGCCACTTGTTGTGAAGCCTGGAATTCAAAAATATCTGCATCTGTCACAGGCGACTCTAGGTGAAATAAAGAAAGACAAAGCGAATGAAAATGTGACAATTTTCTTGAAGATTGGTGGCCAGAAGCTTGTTCTGGGAATTCTCTCCGCAGAGAAGTTTCCCCAGCTATCATTTGATCTCGTGTTTGAGAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAAGTGTTTACTGTGCTGGATACCAGGCTTTCGCGGAGGATCAAGAACAATATCCTTTCTTTTGA

Coding sequence (CDS)

ATGGAGTTCTGGGGTGTTGAAGTTAAGTCTGGTCAGGCACTCTCTGTGCAGCCTGGAGATCAGAAGTATCTGCATCTATCACAGGCGACTCTAGGTGAACTAAAGAAGGATAAAGCGAATGAATCCGTGACAATTTTTGTGAAGATTGGTGACCAGAAGCTTGTTCTGGGAATTCTCTCTGCTGAGAAGTTCCCTCAGCTATCATTTGATCTTGTGTTTGAAAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAATATCCTTCTTTTCCTTAACTTTTATCTCCTGTCTTCCGTGTTCCACTGAAGAGTATTCTGATTCTGATTTTGGCTCAGAAGATGAGGAACTTACATTGCAGCCTGTTCAGAATGGAAAACCTAGTCAGGAGGAGGAGAAGTCTATTGCGGGAAAAAATTCAAAGAAAGCAGAAGTTAAGTCTCTTGTGCCGAGCAAGGAGGAAGGGGATGATGGCTCTGAAGATGATGATGACTCTGATGAGGACGAGGATTCTGATGATGACTCTGATGAGGAAATGCTTGGAGCTGACAGTGACTCTGACGATGAAGATGATGGTACTGAAAGTGAGGAGGAGACACCAAAGAAGGTTGAATCAGCCAAGAAGAGGCCAAACGAGTCTGCCTCGAAGACTCCTGTTTCCAAGAAGGCAAAAGTAGCTTCGGCTGAGAAGACAGGCTCGAAGAAGGGGGGCCATACAGATACTCCCCACCCTGCCAAGAAACCCGGGAAAACTCCTTCCAAGGGAGAAACCCCAAAATCCGCAGGCCAGGTCTCTTGCAAATCCTGTGACAGGTCATTTGGTTCCGACAGTGCTCTTCAGTCGCACAGTAAAGCCAAGCATGGCGGTGTTGAAGTCAAGTCTGGTAAGCCACTTGTTGTGAAGCCTGGAATTCAAAAATATCTGCATCTGTCACAGGCGACTCTAGGTGAAATAAAGAAAGACAAAGCGAATGAAAATGTGACAATTTTCTTGAAGATTGGTGGCCAGAAGCTTGTTCTGGGAATTCTCTCCGCAGAGAAGTTTCCCCAGCTATCATTTGATCTCGTGTTTGAGAAGGAATTTGAGCTCTCCCACAACGGGAAGAGTGGAAGTGTTTACTGTGCTGGATACCAGGCTTTCGCGGAGGATCAAGAACAATATCCTTTCTTTTGA

Protein sequence

MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILSAEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEMLGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVEVKSGKPLVVKPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGGQKLVLGILSAEKFPQLSFDLVFEKEFELSHNGKSGSVYCAGYQAFAEDQEQYPFF
Homology
BLAST of CmaCh20G005500 vs. ExPASy Swiss-Prot
Match: Q6V9I6 (Histone deacetylase HDT1 OS=Solanum chacoense OX=4108 GN=HDT1 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 9.3e-55
Identity = 154/293 (52.56%), Postives = 193/293 (65.87%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG EVKSG+ L+VQPGD   LHLSQA+LGELKKDK +ESV + V I  +KLVLG L+
Sbjct: 1   MEFWGAEVKSGEPLTVQPGDGMVLHLSQASLGELKKDK-SESVCLSVNIDGKKLVLGTLN 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
           +EK PQ  FDLVF+++FELSHN KSG  +F   + +  P   EE  + D+   DE++ L 
Sbjct: 61  SEKVPQQQFDLVFDRDFELSHNLKSGSVYF-FGYKATNPFEEEEDDEDDYDESDEDIPLT 120

Query: 121 PVQNGKPSQEEE-KSIAGKNSK--KAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDE 180
              +GKP  +E  KS AGK+S   K +V+ + P+K+            DEDE SDDD   
Sbjct: 121 LANSGKPEPKEAGKSNAGKDSASGKQKVRIVEPTKD------------DEDESSDDD--- 180

Query: 181 EMLGADSD-SDDEDDGTESEEETPKKVESAKKRPNESASKTPVS-KKAKVASAEKT-GSK 240
                DSD  +DEDD  +SEEETPKK E AK+R  +SA+KTPV+ KKAK+ + +KT G K
Sbjct: 181 -----DSDMGEDEDDSDDSEEETPKKAEPAKRRKADSATKTPVTDKKAKLTTPQKTDGKK 240

Query: 241 KGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKH 288
            GGH  TPHP+K+  K      TPKSAG   CK C+RSFGS+ AL SHSKAKH
Sbjct: 241 GGGHVATPHPSKQASK------TPKSAGSHHCKPCNRSFGSEGALDSHSKAKH 265

BLAST of CmaCh20G005500 vs. ExPASy Swiss-Prot
Match: Q8LJS2 (Histone deacetylase HDT1 OS=Glycine max OX=3847 GN=HDT1 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.6e-54
Identity = 151/300 (50.33%), Postives = 192/300 (64.00%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGD--QKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGI 60
           MEFWGVEVK GQ ++V P D    Y+H+SQ  LGE KKDK NE V +++K+G+QK+VLG 
Sbjct: 1   MEFWGVEVKVGQTVTVDPMDPVDSYIHISQVALGEAKKDKPNEPVVLYLKVGEQKIVLGT 60

Query: 61  LSAEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELT 120
           LS +  P LS DLV + + ELSH  KS   FF    +     +  ++SDS    EDEEL 
Sbjct: 61  LSRDGIPHLSLDLVLDSDSELSHTSKSASVFFCGYKVLTGNDNASDFSDS--SEEDEELA 120

Query: 121 LQPVQNGKPSQEEEKSIAGKNSKK-----AEVKSLVPSKEEGDDGSEDDDDSDEDEDSDD 180
           L+   NGKP  + E +   K SK      A  K+  P K+E DD  ++ DD    ED   
Sbjct: 121 LEGQDNGKPELKAEGAKVTKPSKSIPKIGAPAKAADPKKDEDDDSDDESDDDLAGEDESG 180

Query: 181 DSDEEMLGADSDSDDEDDGTESEEETP-KKVESAKKRPNESASKTPVS-KKAKVASAEKT 240
            SDE  +  DS+S++E DG   +EETP KKV+  KKRPNESA+KTP+S KKAK A+ EKT
Sbjct: 181 SSDE--MDDDSNSEEESDG--DDEETPAKKVDQGKKRPNESAAKTPISAKKAKTATPEKT 240

Query: 241 GSKKGGHTDTPHPAKKPGKTP--SKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
             KK  H  TPHP+KK GKTP  +KG+TP SAGQ+SC SC +SF +++ LQ H KAKHGG
Sbjct: 241 DGKKSVHVATPHPSKKGGKTPNSTKGQTPNSAGQLSCASCKKSFTNEAGLQQHKKAKHGG 294

BLAST of CmaCh20G005500 vs. ExPASy Swiss-Prot
Match: Q9M4U5 (Histone deacetylase HDT2 OS=Zea mays OX=4577 GN=HDT2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.7e-46
Identity = 142/308 (46.10%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG+EVK G  +  +PG    LH+SQA LGE KK   ++S  ++VK+ D+KL +G LS
Sbjct: 1   MEFWGLEVKPGSTVKCEPGHGFILHVSQAALGESKK---SDSALMYVKVDDKKLAIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            +K+PQ+ FDLVF KEFELSH  K+   FFS   +   P   +E  D D   E+EEL + 
Sbjct: 61  IDKYPQIQFDLVFNKEFELSHTSKTTSVFFSGYKVE-QPIEGDE-MDLDSEDEEEELNIP 120

Query: 121 PVQ-----NGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDS 180
            ++     +GK  Q+ ++      + K+ +     SK++ DD  ED+ D  +++DSDD  
Sbjct: 121 VIKENGKADGKEEQKNQEKAVAATASKSSLGLEKKSKDDSDDSDEDESDDSDEDDSDDSD 180

Query: 181 DEEMLG---ADSDSDDEDDGTESEEE---TPKKVESAKKRPNESASKTPVS-KKAKVAS- 240
           + E L     D DS DEDD ++ +EE   TPKK E+ KKR  E+A KTP+S KKAKVA+ 
Sbjct: 181 EGEGLSPDEGDDDSSDEDDTSDDDEEETPTPKKPEAGKKRGAENALKTPLSDKKAKVATP 240

Query: 241 -AEKTGSKKGG-HTDTPHPAKKPGKTPSKGE-----TPKSAGQVSCKSCDRSFGSDSALQ 289
            A+KTG KKG  H  TPHPAK  GKTP+  +     +PKS G V CKSC ++F S+ ALQ
Sbjct: 241 PAQKTGGKKGATHVATPHPAK--GKTPANNDKLTEKSPKSGGSVPCKSCSKTFNSEMALQ 300

BLAST of CmaCh20G005500 vs. ExPASy Swiss-Prot
Match: O24591 (Histone deacetylase HDT1 OS=Zea mays OX=4577 GN=HDT1 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 8.2e-43
Identity = 150/312 (48.08%), Postives = 190/312 (60.90%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG+EVK G  +  +PG    LHLSQA LGE KK   +++  ++VKI DQKL +G LS
Sbjct: 1   MEFWGLEVKPGSTVKCEPGYGFVLHLSQAALGESKK---SDNALMYVKIDDQKLAIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            +K P + FDL+F+KEFELSH  K+   FF  T         E+  D D   EDEEL + 
Sbjct: 61  VDKNPHIQFDLIFDKEFELSHTSKTTSVFF--TGYKVEQPFEEDEMDLDSEDEDEELNVP 120

Query: 121 PV-QNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDE-DEDSDDDSDEE 180
            V +NGK  ++++KS   + +  A  KS   SK+  DD   D+D++D+ DED  DDSDE 
Sbjct: 121 VVKENGKADEKKQKS--QEKAVAAPSKSSPDSKKSKDDDDSDEDETDDSDEDETDDSDEG 180

Query: 181 MLG--ADSDSDDEDDGTESEEE---TPKKVESAKKRPNESA-SKTPVS-KKAKVA--SAE 240
           +     D DS DEDD ++ EEE   TPKK E  KKRP ES+  KTP+S KKAKVA  S++
Sbjct: 181 LSSEEGDDDSSDEDDTSDDEEEDTPTPKKPEVGKKRPAESSVLKTPLSDKKAKVATPSSQ 240

Query: 241 KTGSKKGG--HTDTPHPAKKPGKT--------PSKGETPKSAGQVSCKSCDRSFGSDSAL 292
           KTG KKG   H  TPHPAK  GKT         S    PKS G V CK C +SF S++AL
Sbjct: 241 KTGGKKGAAVHVATPHPAK--GKTIVNNDKSVKSPKSAPKSGGSVPCKPCSKSFISETAL 300

BLAST of CmaCh20G005500 vs. ExPASy Swiss-Prot
Match: Q9M4U4 (Histone deacetylase HDT3 OS=Zea mays OX=4577 GN=HDT3 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.2e-41
Identity = 150/308 (48.70%), Postives = 192/308 (62.34%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG+EVK G  +  +PG    LHLSQA LGE KK   +++  ++VKI DQKL +G LS
Sbjct: 1   MEFWGLEVKPGSTVKCEPGYGFVLHLSQAALGESKK---SDNALMYVKIDDQKLAIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            +K P + FDL+F+KEFELSH  K+   FF  T         E+  D D   EDEEL + 
Sbjct: 61  VDKNPHIQFDLIFDKEFELSHTSKTTSVFF--TGYKVEQPFEEDEMDLDSEDEDEELNVP 120

Query: 121 PV-QNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDE-DEDSDDDSDEE 180
            V +NGK  ++++KS   + +  A  KS   SK+  DD   D+D++D+ DED  DDSDE 
Sbjct: 121 AVKENGKADEKKQKS--QEKAVAAPSKSSPDSKKSKDDDDSDEDETDDSDEDETDDSDEG 180

Query: 181 ML--GADSDSDDEDDGTESEEE---TPKKVESAKKRPNESA-SKTPVS-KKAKVA--SAE 240
           +     D DS DEDD ++ EEE   TPKK E  KKR  ES+  KTP+S KKAKVA  S++
Sbjct: 181 LSPEEGDDDSSDEDDTSDDEEEDTPTPKKPEVGKKRAAESSVLKTPLSDKKAKVATPSSQ 240

Query: 241 KTGSKKGG--HTDTPHPAKKPGKT----PSKGETPKSAGQ--VSCKSCDRSFGSDSALQS 290
           KTG KKG   H  TPHPAK  GKT        ++PKSA +  V CKSC +SF S++A Q+
Sbjct: 241 KTGGKKGAAVHVATPHPAK--GKTIVNNDKSVKSPKSAPKSGVPCKSCSKSFISETAPQA 299

BLAST of CmaCh20G005500 vs. ExPASy TrEMBL
Match: A0A6J1JAV6 (histone deacetylase HDT1-like OS=Cucurbita maxima OX=3661 GN=LOC111484121 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.8e-133
Identity = 276/290 (95.17%), Postives = 278/290 (95.86%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEYSDSDFGSEDEELTL
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYSDSDFGSEDEELTL 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180
           QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM
Sbjct: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180

Query: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240
           LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT
Sbjct: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240

Query: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG
Sbjct: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 289

BLAST of CmaCh20G005500 vs. ExPASy TrEMBL
Match: A0A6J1FXQ5 (histone deacetylase HDT1-like OS=Cucurbita moschata OX=3662 GN=LOC111448147 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.5e-127
Identity = 270/290 (93.10%), Postives = 272/290 (93.79%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEYSDSDFGSEDEELTL
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYSDSDFGSEDEELTL 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180
           QPVQNGKPSQ EEKSIAGKNSKK EVKSLVPSKEEGDD SEDDDDSDEDEDSDDDSDEEM
Sbjct: 121 QPVQNGKPSQ-EEKSIAGKNSKKVEVKSLVPSKEEGDDDSEDDDDSDEDEDSDDDSDEEM 180

Query: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240
           LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT
Sbjct: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240

Query: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           DTPHPAKKP KTPSK ETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG
Sbjct: 241 DTPHPAKKPVKTPSKAETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 288

BLAST of CmaCh20G005500 vs. ExPASy TrEMBL
Match: A0A6J1CG52 (histone deacetylase HDT1-like OS=Momordica charantia OX=3673 GN=LOC111011215 PE=3 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 7.1e-106
Identity = 230/296 (77.70%), Postives = 254/296 (85.81%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQAL+V+PGDQKYLHLSQATLGE+KKDKANESVTIF+KIGDQKLVLG+LS
Sbjct: 1   MEFWGVEVKPGQALAVKPGDQKYLHLSQATLGEIKKDKANESVTIFLKIGDQKLVLGVLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEY DSDFGSEDEEL L
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYLDSDFGSEDEELAL 120

Query: 121 QPVQNGKPSQEEE------KSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDD 180
            P QNGKPSQ+E+       ++  ++SKKA+VK L PSKE+ DD SEDDD+SDED+DSDD
Sbjct: 121 HPAQNGKPSQKEKAIVEKLNAMKPESSKKADVKPLDPSKEDEDDDSEDDDESDEDDDSDD 180

Query: 181 DSDEEMLGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGS 240
           +SDEEMLGADSDSDDEDDGTES+EETPKKVES KKR N+SASKTP+SKKAK+ASAEKT +
Sbjct: 181 ESDEEMLGADSDSDDEDDGTESDEETPKKVESNKKRSNDSASKTPLSKKAKLASAEKTDA 240

Query: 241 KKGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           KKGGHT TPHPAKKP KT  K ETPKS GQ SCKSCD+SFGSD ALQSHSKAKH G
Sbjct: 241 KKGGHTATPHPAKKPAKTAGKTETPKSGGQFSCKSCDKSFGSDGALQSHSKAKHAG 295

BLAST of CmaCh20G005500 vs. ExPASy TrEMBL
Match: A0A6J1H533 (histone deacetylase HDT1-like OS=Cucurbita moschata OX=3662 GN=LOC111459656 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 3.1e-101
Identity = 229/297 (77.10%), Postives = 246/297 (82.83%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSV+PGDQKYLHLSQATLGELKKDK+ ESVTIF+KI +QKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVKPGDQKYLHLSQATLGELKKDKSKESVTIFLKIDEQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
           AEKFPQLSFDLVFEKEFELSHNGKSG  +      S      EEYSDSDFGSEDEEL LQ
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSGSIYCMGYQASAEDQEHEEYSDSDFGSEDEELALQ 120

Query: 121 PVQNGKPSQEEEKSIAGKN-------SKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDD 180
            V NGKPSQ+EEKSI GK+       SKK   KSL PSKE+ DD SEDDD+SD DEDS D
Sbjct: 121 HVPNGKPSQKEEKSIVGKHSSLKPETSKKTHSKSLEPSKEDEDDDSEDDDESD-DEDSSD 180

Query: 181 DSDEEMLGADSDSDDEDDGTESEEETPK-KVESAKKRPNESASKTPVSKKAKVASAEKTG 240
           +SDEEMLG+DSDSDDEDD  ESEEETPK KVES KKR N+SAS TPVSKK+K+ASAEK+ 
Sbjct: 181 ESDEEMLGSDSDSDDEDDDIESEEETPKRKVESNKKRSNDSASVTPVSKKSKLASAEKSD 240

Query: 241 SKKGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           +KKGGHT TPHPAKKP KTP+K  TPKS GQ SCKSCDRSFGSD ALQSH KAKHGG
Sbjct: 241 AKKGGHTATPHPAKKPVKTPAKAATPKSGGQFSCKSCDRSFGSDGALQSHGKAKHGG 296

BLAST of CmaCh20G005500 vs. ExPASy TrEMBL
Match: A0A0A0KGM8 (Histone deacetylase 2a OS=Cucumis sativus OX=3659 GN=Csa_6G150550 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.6e-97
Identity = 223/297 (75.08%), Postives = 244/297 (82.15%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQ LSVQPGD  Y+HLSQATLG+LKKDKANE VTIF+KI DQKLVLG+LS
Sbjct: 1   MEFWGVEVKPGQPLSVQPGDMNYVHLSQATLGDLKKDKANEPVTIFLKIDDQKLVLGVLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCL-PCSTEEYSDSDFGSEDEELTL 120
           A+KFPQ+SFDLVFEKEFELSHNGK G S + L + + +     EE+SDSDFGSEDEEL +
Sbjct: 61  ADKFPQISFDLVFEKEFELSHNGKGG-SIYCLGYRAPMEDQGQEEFSDSDFGSEDEELGM 120

Query: 121 QPVQNGKPSQEEEKSIAGK-------NSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSD 180
              +NGK S E+EKSI GK       +SKKA+ KS+ PSKEE DD SEDDD SDE  DSD
Sbjct: 121 LSAENGKAS-EKEKSIGGKIFGLKPESSKKADAKSIAPSKEE-DDDSEDDDSSDEGSDSD 180

Query: 181 DDSDEEMLGADSDSDDEDDGTESEEETPKKV-ESAKKRPNESASKTPVSKKAKVASAEKT 240
           D SDEEMLG DSDSDDEDDGT+SEEETPKKV ES+KKR NESASKTPVSKK K+ASAEKT
Sbjct: 181 DVSDEEMLGGDSDSDDEDDGTDSEEETPKKVNESSKKRSNESASKTPVSKKTKLASAEKT 240

Query: 241 GSKKGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHG 289
            SKKGGHT TPHPAKKP K+P K ETPKS GQ SC SCDRSFGSD ALQSHSKAKHG
Sbjct: 241 DSKKGGHTATPHPAKKPAKSPGKAETPKSGGQFSCTSCDRSFGSDGALQSHSKAKHG 294

BLAST of CmaCh20G005500 vs. NCBI nr
Match: KAG6570843.1 (Histone deacetylase HDT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.8 bits (1662), Expect = 4.7e-181
Identity = 367/402 (91.29%), Postives = 370/402 (92.04%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEYSDSDFGSEDEELTL
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYSDSDFGSEDEELTL 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180
           QPVQNGKPSQ EEKSIAGKNSKKAEVKSLVPSKEEGDD SEDDDDSDEDEDSDDDSDEEM
Sbjct: 121 QPVQNGKPSQ-EEKSIAGKNSKKAEVKSLVPSKEEGDDDSEDDDDSDEDEDSDDDSDEEM 180

Query: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240
           LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT
Sbjct: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240

Query: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHG----------- 300
           DTPHPAKKP KTPSK ETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHG           
Sbjct: 241 DTPHPAKKPVKTPSKAETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGASSTYSSMEFW 300

Query: 301 GVEVKSGKPLVVKPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGGQKLVLGILSAEKF 360
           GVEVKSGKPLVVKPGIQKYLHLSQATLGEIKKDKANENVTIFLKIG QKLVLGILSAEKF
Sbjct: 301 GVEVKSGKPLVVKPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGDQKLVLGILSAEKF 360

Query: 361 PQLSFDLVFEKEFELSHNGKSGSVYCAGYQAFAEDQEQYPFF 391
           PQLSFDLVFEKEFELSHNGKSGSVYCAGYQAFAEDQE   +F
Sbjct: 361 PQLSFDLVFEKEFELSHNGKSGSVYCAGYQAFAEDQEHDGYF 400

BLAST of CmaCh20G005500 vs. NCBI nr
Match: KAG7010689.1 (Histone deacetylase HDT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 614.4 bits (1583), Expect = 6.9e-172
Identity = 349/385 (90.65%), Postives = 354/385 (91.95%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
           AEKFPQLSFDLVFEKEFELSHNGKSG                    +SDFGSEDEELTLQ
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG--------------------NSDFGSEDEELTLQ 120

Query: 121 PVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEML 180
           PVQNGKPSQ EEKSIAGKNSKKAEVKSLVPSKEEGDD SEDDDDSDEDEDSDDDSDEEML
Sbjct: 121 PVQNGKPSQ-EEKSIAGKNSKKAEVKSLVPSKEEGDDDSEDDDDSDEDEDSDDDSDEEML 180

Query: 181 GADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHTD 240
           GADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHTD
Sbjct: 181 GADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHTD 240

Query: 241 TPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVEVKSGKPLVV 300
           TPHPAKKP KTPSK ETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVEVKSGKPLVV
Sbjct: 241 TPHPAKKPVKTPSKAETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVEVKSGKPLVV 300

Query: 301 KPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGGQKLVLGILSAEKFPQLSFDLVFEKE 360
           KPGIQKYLHLSQATLGEIKKDKANENVTIFLKIG QKLVLGILSAEKFPQLSFDLVFEKE
Sbjct: 301 KPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGDQKLVLGILSAEKFPQLSFDLVFEKE 360

Query: 361 FELSHNGKSGSVYCAGYQAFAEDQE 386
           FELSHNGKSGSVY +   + +ED+E
Sbjct: 361 FELSHNGKSGSVYYS--DSGSEDEE 362

BLAST of CmaCh20G005500 vs. NCBI nr
Match: KAG7035961.1 (Histone deacetylase HDT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.8 bits (1392), Expect = 9.6e-150
Identity = 313/380 (82.37%), Postives = 332/380 (87.37%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSV+PGDQKYLHLSQATLGELKKDK+ ESVTIF+KI +QKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVKPGDQKYLHLSQATLGELKKDKSKESVTIFLKIDEQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
           AEKFPQLSFDLVFEKEFELSHNGKSGIS  SLT ISCL  STEEYSDSDFGSEDEEL LQ
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISVSSLTLISCLLFSTEEYSDSDFGSEDEELALQ 120

Query: 121 PVQNGKPSQEEEKSIAGKN-------SKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDD 180
            V NGK SQ+EEKSI GK+       SKK   KSL PSKE+ DD SEDDD+SD DEDS D
Sbjct: 121 HVPNGKSSQKEEKSIVGKHSSLKPETSKKTHSKSLEPSKEDEDDDSEDDDESD-DEDSSD 180

Query: 181 DSDEEMLGADSDSDDEDDGTESEEETPK-KVESAKKRPNESASKTPVSKKAKVASAEKTG 240
           +SDEEMLG+DSDSDDEDD  ESEEETPK KVES KKR N+SAS TPVSKK+K+ASAEK+ 
Sbjct: 181 ESDEEMLGSDSDSDDEDDDIESEEETPKRKVESNKKRSNDSASVTPVSKKSKLASAEKSD 240

Query: 241 SKKGGHTDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKH-GGVE 300
           +KKGGHT TPHPAKKP KTP+K  TPKS GQ SCKSCDRSFGSD ALQSH KAKH GGVE
Sbjct: 241 AKKGGHTATPHPAKKPVKTPAKAATPKSGGQFSCKSCDRSFGSDGALQSHGKAKHGGGVE 300

Query: 301 VKSGKPLVVKPGIQKYLHLSQATLGEIKKDKANENVTIFLKIGGQKLVLGILSAEKFPQL 360
           VK G+ LVVKPGIQ+YLHLSQATLGE KKDKANENVTIFLKIGGQKLVLGILSAEKFPQL
Sbjct: 301 VKPGQSLVVKPGIQQYLHLSQATLGETKKDKANENVTIFLKIGGQKLVLGILSAEKFPQL 360

Query: 361 SFDLVFEKEFELSHNGKSGS 372
           SFDLVFEKEFELSHNGK G+
Sbjct: 361 SFDLVFEKEFELSHNGKGGN 379

BLAST of CmaCh20G005500 vs. NCBI nr
Match: XP_022986356.1 (histone deacetylase HDT1-like [Cucurbita maxima])

HSP 1 Score: 483.4 bits (1243), Expect = 1.8e-132
Identity = 276/290 (95.17%), Postives = 278/290 (95.86%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEYSDSDFGSEDEELTL
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYSDSDFGSEDEELTL 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180
           QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM
Sbjct: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180

Query: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240
           LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT
Sbjct: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240

Query: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG
Sbjct: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 289

BLAST of CmaCh20G005500 vs. NCBI nr
Match: XP_023513046.1 (histone deacetylase HDT1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 471.5 bits (1212), Expect = 7.2e-129
Identity = 271/290 (93.45%), Postives = 273/290 (94.14%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK GQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS
Sbjct: 1   MEFWGVEVKPGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTF-ISCLPCSTEEYSDSDFGSEDEELTL 120
           AEKFPQLSFDLVFEKEFELSHNGKSG S + L +  S      EEYSDSDFGSEDEELTL
Sbjct: 61  AEKFPQLSFDLVFEKEFELSHNGKSG-SIYCLGYQASAEDQEHEEYSDSDFGSEDEELTL 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEM 180
           QPVQNGKPSQEEEKSI GKNSKKAEVKSLVPSKEEGDD SEDDDDSDEDEDSDDDSDEEM
Sbjct: 121 QPVQNGKPSQEEEKSIVGKNSKKAEVKSLVPSKEEGDDDSEDDDDSDEDEDSDDDSDEEM 180

Query: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240
           LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT
Sbjct: 181 LGADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVSKKAKVASAEKTGSKKGGHT 240

Query: 241 DTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 290
           DTPHPAKKP KTPSK ETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG
Sbjct: 241 DTPHPAKKPVKTPSKAETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGG 289

BLAST of CmaCh20G005500 vs. TAIR 10
Match: AT5G03740.1 (histone deacetylase 2C )

HSP 1 Score: 149.8 bits (377), Expect = 4.5e-36
Identity = 124/304 (40.79%), Postives = 174/304 (57.24%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGVEVK+G+ L + PG  + +H+SQ  LGE  K+   E + ++V +G  KL++G LS
Sbjct: 1   MEFWGVEVKNGKPLHLDPGLDRLVHISQVALGE-SKNNVTEPIQLYVTVGSDKLLIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            EKFPQLS ++V E+ F LSH  K+G  FFS             Y       E E+L + 
Sbjct: 61  HEKFPQLSTEIVLERNFALSHTWKNGSVFFS------------GYKVDASDPEPEDL-ID 120

Query: 121 PVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEML 180
                   +   KS A + + +   + +   +++  DGSE+D   D+D ++  D +EE +
Sbjct: 121 DQLEAAGFKAAPKSAAKQVNFQLPNEDVKAKQDDDADGSEEDSSDDDDSENSGDEEEEKV 180

Query: 181 GADSDSDDEDDGTE-----SEEETPKKVESAKKRPNE-SASKTPVS-KKAKVASAEKTGS 240
            A+SDS+++D   +     SEEETPKK E  KKR  E ++SK P S KKAK  + +KT S
Sbjct: 181 TAESDSEEDDSSDDEEDDSSEEETPKKPEEPKKRSAEPNSSKNPASNKKAKFVTPQKTDS 240

Query: 241 KKGG-HTDTPHPAKKPGK---------TPSKGETPKSAGQVSCKSCDRSFGSDSALQSHS 288
           KK   H  TPHP+K+ GK         T  + +TPKSAG   CKSC R+F S+  LQSH+
Sbjct: 241 KKPHVHVATPHPSKQAGKNSGGGSTGETSKQQQTPKSAGAFGCKSCTRTFTSEMGLQSHT 290

BLAST of CmaCh20G005500 vs. TAIR 10
Match: AT5G22650.2 (histone deacetylase 2B )

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-35
Identity = 127/283 (44.88%), Postives = 165/283 (58.30%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGV V    A  V P +   +H+SQA+L      K+ ESV + V +G  KLV+G LS
Sbjct: 1   MEFWGVAVTPKNATKVTPEEDSLVHISQASLD--CTVKSGESVVLSVTVGGAKLVIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNG-KSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTL 120
            +KFPQ+SFDLVF+KEFELSH+G K+ + F            +      DF S D+E   
Sbjct: 61  QDKFPQISFDLVFDKEFELSHSGTKANVHFIGY--------KSPNIEQDDFTSSDDEDVP 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVK------SLVPSKEEGDDGSEDDDDSDEDEDSDD 180
           + V    P+       AG    KA+ K       + P++E+ +   ED+ D +++ + DD
Sbjct: 121 EAVPAPAPTAVTANGNAGAAVVKADTKPKAKPAEVKPAEEKPESDEEDESDDEDESEEDD 180

Query: 181 DSDEEMLGADSDSDD---EDDGTESEEETPKKVESA-KKRPNESASKTPVS-KKAKVASA 240
           DS++ M   + DSDD   ED   E EEETPKK E   KKRPNES SKTPVS KKAK A+A
Sbjct: 181 DSEKGMDVDEDDSDDDEEEDSEDEEEEETPKKPEPINKKRPNESVSKTPVSGKKAKPAAA 240

Query: 241 -----EKTGSKKGGHTDTPHPAKKPGKTP-SKGETPKSAGQVS 266
                +KT  KKGGHT TPHPAKK GK+P +  ++PKS GQ S
Sbjct: 241 PASTPQKTEKKKGGHTATPHPAKKGGKSPVNANQSPKSGGQSS 273

BLAST of CmaCh20G005500 vs. TAIR 10
Match: AT3G44750.2 (histone deacetylase 3 )

HSP 1 Score: 144.8 bits (364), Expect = 1.4e-34
Identity = 125/293 (42.66%), Postives = 162/293 (55.29%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG+EVKSG+ ++V P +   +H+SQA+LGE  K+K  E V + VK+G+Q LVLG LS
Sbjct: 1   MEFWGIEVKSGKPVTVTPEEGILIHVSQASLGEC-KNKKGEFVPLHVKVGNQNLVLGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            E  PQL  DLVF+KEFELSH    G  +F       +   T       +  E+EE    
Sbjct: 61  TENIPQLFCDLVFDKEFELSHTWGKGSVYF-------VGYKTPNIEPQGYSEEEEEEV-- 120

Query: 121 PVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEML 180
                 P+    K++A   +K AEVK  V              D +EDE   D  DE+  
Sbjct: 121 ------PAGNAAKAVAKPKAKPAEVKPAV--------------DDEEDESDSDGMDED-- 180

Query: 181 GADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVS-KKAKVA-SAEKTGSKKGGH 240
               DSD ED  +E EE TPKK  S+KKR NE+  K PVS KKAKVA + +KT  KK G 
Sbjct: 181 ----DSDGED--SEEEEPTPKKPASSKKRANETTPKAPVSAKKAKVAVTPQKTDEKKKG- 240

Query: 241 TDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVE 292
                     GK  +  ++PKSA QVSC SC ++F S +AL+SH+KAKH   +
Sbjct: 241 ----------GK--AANQSPKSASQVSCGSCKKTFNSGNALESHNKAKHAAAK 242

BLAST of CmaCh20G005500 vs. TAIR 10
Match: AT3G44750.1 (histone deacetylase 3 )

HSP 1 Score: 144.4 bits (363), Expect = 1.9e-34
Identity = 125/293 (42.66%), Postives = 163/293 (55.63%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWG+EVKSG+ ++V P +   +H+SQA+LGE  K+K  E V + VK+G+Q LVLG LS
Sbjct: 1   MEFWGIEVKSGKPVTVTPEEGILIHVSQASLGEC-KNKKGEFVPLHVKVGNQNLVLGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNGKSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTLQ 120
            E  PQL  DLVF+KEFELSH    G  +F       +   T       +  E+EE    
Sbjct: 61  TENIPQLFCDLVFDKEFELSHTWGKGSVYF-------VGYKTPNIEPQGYSEEEEE---- 120

Query: 121 PVQNGKPSQEEEKSIAGKNSKKAEVKSLVPSKEEGDDGSEDDDDSDEDEDSDDDSDEEML 180
             +   P+    K++A   +K AEVK  V              D +EDE   D  DE+  
Sbjct: 121 -EEEEVPAGNAAKAVAKPKAKPAEVKPAV--------------DDEEDESDSDGMDED-- 180

Query: 181 GADSDSDDEDDGTESEEETPKKVESAKKRPNESASKTPVS-KKAKVA-SAEKTGSKKGGH 240
               DSD ED  +E EE TPKK  S+KKR NE+  K PVS KKAKVA + +KT  KK G 
Sbjct: 181 ----DSDGED--SEEEEPTPKKPASSKKRANETTPKAPVSAKKAKVAVTPQKTDEKKKG- 240

Query: 241 TDTPHPAKKPGKTPSKGETPKSAGQVSCKSCDRSFGSDSALQSHSKAKHGGVE 292
                     GK  +  ++PKSA QVSC SC ++F S +AL+SH+KAKH   +
Sbjct: 241 ----------GK--AANQSPKSASQVSCGSCKKTFNSGNALESHNKAKHAAAK 245

BLAST of CmaCh20G005500 vs. TAIR 10
Match: AT5G22650.1 (histone deacetylase 2B )

HSP 1 Score: 143.7 bits (361), Expect = 3.2e-34
Identity = 127/284 (44.72%), Postives = 165/284 (58.10%), Query Frame = 0

Query: 1   MEFWGVEVKSGQALSVQPGDQKYLHLSQATLGELKKDKANESVTIFVKIGDQKLVLGILS 60
           MEFWGV V    A  V P +   +H+SQA+L      K+ ESV + V +G  KLV+G LS
Sbjct: 1   MEFWGVAVTPKNATKVTPEEDSLVHISQASLD--CTVKSGESVVLSVTVGGAKLVIGTLS 60

Query: 61  AEKFPQLSFDLVFEKEFELSHNG-KSGISFFSLTFISCLPCSTEEYSDSDFGSEDEELTL 120
            +KFPQ+SFDLVF+KEFELSH+G K+ + F            +      DF S D+E   
Sbjct: 61  QDKFPQISFDLVFDKEFELSHSGTKANVHFIGY--------KSPNIEQDDFTSSDDEDVP 120

Query: 121 QPVQNGKPSQEEEKSIAGKNSKKAEVK------SLVPSKEEGDDGSEDDDDSDEDEDSDD 180
           + V    P+       AG    KA+ K       + P++E+ +   ED+ D +++ + DD
Sbjct: 121 EAVPAPAPTAVTANGNAGAAVVKADTKPKAKPAEVKPAEEKPESDEEDESDDEDESEEDD 180

Query: 181 DSDEEMLGADSDSDD---EDDGTESEEETPKKVESA-KKRPNESASKTPVS-KKAKVASA 240
           DS++ M   + DSDD   ED   E EEETPKK E   KKRPNES SKTPVS KKAK A+A
Sbjct: 181 DSEKGMDVDEDDSDDDEEEDSEDEEEEETPKKPEPINKKRPNESVSKTPVSGKKAKPAAA 240

Query: 241 -----EKT-GSKKGGHTDTPHPAKKPGKTP-SKGETPKSAGQVS 266
                +KT   KKGGHT TPHPAKK GK+P +  ++PKS GQ S
Sbjct: 241 PASTPQKTEEKKKGGHTATPHPAKKGGKSPVNANQSPKSGGQSS 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6V9I69.3e-5552.56Histone deacetylase HDT1 OS=Solanum chacoense OX=4108 GN=HDT1 PE=2 SV=1[more]
Q8LJS21.6e-5450.33Histone deacetylase HDT1 OS=Glycine max OX=3847 GN=HDT1 PE=2 SV=1[more]
Q9M4U52.7e-4646.10Histone deacetylase HDT2 OS=Zea mays OX=4577 GN=HDT2 PE=1 SV=1[more]
O245918.2e-4348.08Histone deacetylase HDT1 OS=Zea mays OX=4577 GN=HDT1 PE=1 SV=1[more]
Q9M4U41.2e-4148.70Histone deacetylase HDT3 OS=Zea mays OX=4577 GN=HDT3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JAV68.8e-13395.17histone deacetylase HDT1-like OS=Cucurbita maxima OX=3661 GN=LOC111484121 PE=3 S... [more]
A0A6J1FXQ52.5e-12793.10histone deacetylase HDT1-like OS=Cucurbita moschata OX=3662 GN=LOC111448147 PE=3... [more]
A0A6J1CG527.1e-10677.70histone deacetylase HDT1-like OS=Momordica charantia OX=3673 GN=LOC111011215 PE=... [more]
A0A6J1H5333.1e-10177.10histone deacetylase HDT1-like OS=Cucurbita moschata OX=3662 GN=LOC111459656 PE=3... [more]
A0A0A0KGM81.6e-9775.08Histone deacetylase 2a OS=Cucumis sativus OX=3659 GN=Csa_6G150550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6570843.14.7e-18191.29Histone deacetylase HDT1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7010689.16.9e-17290.65Histone deacetylase HDT1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG7035961.19.6e-15082.37Histone deacetylase HDT1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022986356.11.8e-13295.17histone deacetylase HDT1-like [Cucurbita maxima][more]
XP_023513046.17.2e-12993.45histone deacetylase HDT1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G03740.14.5e-3640.79histone deacetylase 2C [more]
AT5G22650.21.3e-3544.88histone deacetylase 2B [more]
AT3G44750.21.4e-3442.66histone deacetylase 3 [more]
AT3G44750.11.9e-3442.66histone deacetylase 3 [more]
AT5G22650.13.2e-3444.72histone deacetylase 2B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.340Nucleoplasmin core domaincoord: 283..384
e-value: 1.2E-12
score: 49.9
coord: 1..93
e-value: 1.5E-13
score: 52.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..253
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 155..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 105..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 196..215
NoneNo IPR availablePANTHERPTHR3180232 KDA HEAT SHOCK PROTEIN-RELATEDcoord: 289..382
coord: 1..285
NoneNo IPR availablePANTHERPTHR31802:SF12HISTONE DEACETYLASE HDT3-LIKE ISOFORM X1coord: 289..382
coord: 1..285
IPR041232Nucleoplasmin-like domainPFAMPF17800NPLcoord: 3..80
e-value: 1.5E-9
score: 38.4
coord: 289..377
e-value: 1.5E-8
score: 35.3
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 266..287
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 264..292
score: 10.179878

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh20G005500.1CmaCh20G005500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0010162 seed dormancy process
cellular_component GO:0005730 nucleolus