CmaCh20G002070 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh20G002070 vs. ExPASy Swiss-Prot
Match: Q54HG4 (RNA polymerase II subunit 5-mediating protein homolog OS=Dictyostelium discoideum OX=44689 GN=rmp PE=3 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 2.1e-17
Identity = 81/272 (29.78%), Postives = 140/272 (51.47%), Query Frame = 0

Query: 59  RVQDAISEKQRELEHLRGFISDNANLINLVQKLPEELHHEVMVPFGKAAFFPGRLVHTNE 118
           + +  + E Q  +E L+   +D   LI+ +Q LP+ L  ++MVP GK AFF G L +TNE
Sbjct: 9   KYEQKVVEYQDHIEELKSTKNDYKQLIDTLQHLPDTLQPKIMVPMGKLAFFEGNLKNTNE 68

Query: 119 FLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSLKAMMDDLKAEAS----FFDATASE 178
            L+LLG+ Y+A+R+SKQT++I++RR K +D+ ++ L+  +  LK   S       A   +
Sbjct: 69  ILILLGDNYFAKRSSKQTIDIIQRRDKDIDTSINDLREQIKGLKQRVSMTTDLSKALHEK 128

Query: 179 AAEGLVEITEEYVEENNSEQESGSGVNKQDA-----PSVSGVDEARITEE-------DEE 238
             + +VEI EEY  +   E+E       Q +      + +  D+ +  EE       DEE
Sbjct: 129 EYDNIVEIKEEYNSDEEREKEKKRKQKPQKSTTTTTTTTTSKDKPKTEEEKKKSKEMDEE 188

Query: 239 YARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSKASTFPCQR 298
           + +++ RL  LEE+E     N   +D + EE  +  N+ L+ +  +   Y   +      
Sbjct: 189 FDKMLKRLSILEEKE-----NKMGDDYDEEEFNKKFNKKLD-ITGSDEEYDDDNYNNNND 248

Query: 299 DEDIGSKELLDKYQQQQQEASTNPSNCSNFSE 315
           D+D   ++   +Y Q++      P N SN+++
Sbjct: 249 DDDDNDEDDDREYYQEEGFEDEKPEN-SNYNK 273

BLAST of CmaCh20G002070 vs. ExPASy Swiss-Prot
Match: O94763 (Unconventional prefoldin RPB5 interactor 1 OS=Homo sapiens OX=9606 GN=URI1 PE=1 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 2.0e-15
Identity = 77/275 (28.00%), Postives = 131/275 (47.64%), Query Frame = 0

Query: 50  AQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEELHHEVMVPFGKAAFF 109
           A D  +     +  ++  Q  ++H +   +D   L   +  LP++L + +MVPFG  AF 
Sbjct: 27  APDVARLREEQEKVVTNCQERIQHWKKVDNDYNALRERLSTLPDKLSYNIMVPFGPFAFM 86

Query: 110 PGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSLKAMMDDLKAEASFFD 169
           PG+LVHTNE  VLLG+ ++A+ ++KQ V +++ R + +   +D LK +M + ++   F +
Sbjct: 87  PGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNFESRVEFTE 146

Query: 170 --ATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEARITEEDEEY---- 229
                S+AA  +V+I EE   +   + +          P  S + EA I  + +      
Sbjct: 147 DLQKMSDAAGDIVDIREEIKCDFEFKAKHRIAHKPHSKPKTSDIFEADIANDVKSKDLLA 206

Query: 230 -ARIMSRLDELEEEEELAAANDNQND---------EEGEEEKETSNQSLEKLHDAKHSYS 289
              + +RL+ELE +EEL    D++ D            EEEKE  N ++  +H    S++
Sbjct: 207 DKELWARLEELERQEELLGELDSKPDTVIANGEDTTSSEEEKEDRNTNVNAMHQVTDSHT 266

Query: 290 KASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSN 309
                PC +  D+ S E        Q   S N S+
Sbjct: 267 -----PCHK--DVASSEPFSGQVNSQLNCSVNGSS 294

BLAST of CmaCh20G002070 vs. ExPASy Swiss-Prot
Match: Q3B7M7 (Unconventional prefoldin RPB5 interactor OS=Bos taurus OX=9913 GN=URI1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-12
Identity = 79/307 (25.73%), Postives = 141/307 (45.93%), Query Frame = 0

Query: 42  ASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEELHHEVMV 101
           AS ++   A +  +     +  ++  Q +++H +   +D   L   +  LP++L + +MV
Sbjct: 11  ASAAAPLRAPEVARLREEQEKVVTNCQEKIQHWKKVDNDYNALQERLSTLPDKLSYNIMV 70

Query: 102 PFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSLKAMMDDL 161
           PFG  AF PG+LVHTNE  VLLG+ ++A+ ++KQ V +++ R + +   +D LK +M + 
Sbjct: 71  PFGPFAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNF 130

Query: 162 KAEASFFD--ATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEARITEE 221
           ++   F +     S+AA  +V+I EE   +   + +          P  S + EA    +
Sbjct: 131 ESRVEFTEDLQKMSDAAGDIVDIREEIKTDFEFKAKHRIAHKPHSKPKTSDIFEAEFAND 190

Query: 222 --------DEEYARIMSRLDELEEEEELAAAND----------NQNDEEGEEEKETSNQS 281
                   D+E   +  RL+ELE +EEL    D          N  D   EEEKE  N +
Sbjct: 191 LKSKDLLADKE---LWDRLEELERQEELLGEIDIDSKPDTVIANGEDVSSEEEKEDQNIN 250

Query: 282 LEKLHDAKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGS 329
           +  +H    S + +S +       + + EL +         S N S+  + +E       
Sbjct: 251 VNMMHQVTDSLALSSCY-----NSLTNSELFNGQVNSPLNYSVNGSSSYHSNEDDGDNND 309

BLAST of CmaCh20G002070 vs. ExPASy Swiss-Prot
Match: Q3TLD5 (Unconventional prefoldin RPB5 interactor OS=Mus musculus OX=10090 GN=Uri1 PE=1 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 7.8e-12
Identity = 69/260 (26.54%), Postives = 128/260 (49.23%), Query Frame = 0

Query: 41  VASLSSLFPAQDAHKAAMRVQD--AISEKQRELEHLRGFISDNANLINLVQKLPEELHHE 100
           +A  S+  P +    A +R +    ++  Q +++H     +D + L   ++ LP++L ++
Sbjct: 14  LAEASAAAPLRAPEVARLREEQEKVVTNCQEKIQHWEKVDNDYSALQERLRTLPDKLSYD 73

Query: 101 VMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSLKAMM 160
           VMVPFG  AF PG+LVHTNE  VLLG+ ++A+ ++KQ V +++ R + +   +D  K ++
Sbjct: 74  VMVPFGPLAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDFKKVL 133

Query: 161 DDLKAEASFFD--ATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEARI 220
            + ++   F +     S+AA   V+I EE   +   + +          P  S + EA  
Sbjct: 134 KNFESRVEFTEDLQKMSDAAGDFVDIREEIKSDFEFKGKQRIAHKPHSKPKTSDIFEADF 193

Query: 221 -----TEEDEEYARIMSRLDELEEEEELAAANDNQNDE---------EGEEEKETSNQSL 280
                 +   +   + +RL+ELE +EEL    +++ D            EEEKE ++  +
Sbjct: 194 ENGVKPKNTFDADELWARLEELERQEELLGELESKPDTVIANGEDRVSSEEEKEGADTGV 253

Query: 281 EKLHDAKHSYSKASTFPCQR 283
             +     S + +S   C+R
Sbjct: 254 NVVSPVTDSSAASS---CKR 270

BLAST of CmaCh20G002070 vs. ExPASy Swiss-Prot
Match: Q9W148 (Unconventional prefoldin RPB5 interactor-like protein OS=Drosophila melanogaster OX=7227 GN=uri PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.5e-04
Identity = 80/284 (28.17%), Postives = 127/284 (44.72%), Query Frame = 0

Query: 63  AISEKQRELEHLRGFISDNANLINLVQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVL 122
           A+     E E    F  DN + I  + K  + L  EVMVP G+ A  PG L+HTNE LV 
Sbjct: 11  ALQTNASETERWEAFKRDNESTIRNLDKFAKNLSVEVMVPIGRKALMPGELIHTNELLVG 70

Query: 123 LGEGYYAERTSKQTVEILKRRGKALDSQVDSLKAMMDDL---KAEASFFDATASEAAEGL 182
             EGY++  +S +  EI + R K  + Q+  L A+ +DL   K    F +       +  
Sbjct: 71  HYEGYFSACSSHKAKEICQYRLKLAEEQLKKL-AVENDLWQKKLHTPFAEGAVPSGDQ-- 130

Query: 183 VEITEEYVEENNSEQESGSGVNKQDAPSVSGVD-EARITEEDEEYARIMSRLDELEEEEE 242
           VEI E++ EE++++  +      +       ++ EA   ++D E   ++ +L+E E  EE
Sbjct: 131 VEIVEDFNEESHNKWLAEHRKRMRQQKQKERLEREAEPVKKDNE---VLRKLEEREMMEE 190

Query: 243 LAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSKASTFPCQRDEDIGSKELLDKYQQQ 302
           L    DN ++   ++  +  NQ  E L     S  K+ T   Q +ED   K+L  + Q +
Sbjct: 191 LGLDPDNIDE---DQLHDMLNQ--EPLKSTNESSPKSLT---QEEEDELWKKLEAEEQNE 250

Query: 303 QQEASTNPSN------------CSNFSEQSSSKGSLHGNNSSTQ 331
             E S+                 S  +E  SSK    G N + +
Sbjct: 251 ADELSSEAEESLKTTDNLVRQLMSGETETPSSKKRTAGTNRNVE 280

BLAST of CmaCh20G002070 vs. ExPASy TrEMBL
Match: A0A6J1J6D1 (RNA polymerase II subunit 5-mediating protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111483860 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.6e-196
Identity = 378/378 (100.00%), Postives = 378/378 (100.00%), Query Frame = 0

Query: 29  MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV 88
           MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV
Sbjct: 1   MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV 60

Query: 89  QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD 148
           QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD
Sbjct: 61  QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD 120

Query: 149 SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV 208
           SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV
Sbjct: 121 SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV 180

Query: 209 SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA 268
           SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA
Sbjct: 181 SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA 240

Query: 269 KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS 328
           KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS
Sbjct: 241 KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS 300

Query: 329 TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ 388
           TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ
Sbjct: 301 TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ 360

Query: 389 PSGSQPSKPVSRFKMQRR 407
           PSGSQPSKPVSRFKMQRR
Sbjct: 361 PSGSQPSKPVSRFKMQRR 378

BLAST of CmaCh20G002070 vs. ExPASy TrEMBL
Match: A0A6J1FUU9 (RNA polymerase II subunit 5-mediating protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111448325 PE=4 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 9.1e-189
Identity = 368/379 (97.10%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 29  MIME-GGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINL 88
           MIME GGGGAKGTVA+LSSLFPAQDAHKAAMRVQDAISEKQRELEHL GFISDNANLINL
Sbjct: 1   MIMEGGGGGAKGTVAALSSLFPAQDAHKAAMRVQDAISEKQRELEHLLGFISDNANLINL 60

Query: 89  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 148
           VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL
Sbjct: 61  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 120

Query: 149 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPS 208
           DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEE+NSEQESGSGVNKQDAPS
Sbjct: 121 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGSGVNKQDAPS 180

Query: 209 VSGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHD 268
           VSGVDEARITEEDEEYARIMSRLDELE+EEELAAANDNQNDE+GEEE ETSNQSLEKLHD
Sbjct: 181 VSGVDEARITEEDEEYARIMSRLDELEKEEELAAANDNQNDEKGEEENETSNQSLEKLHD 240

Query: 269 AKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNS 328
           AKHSYSKAST PC+RDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGN+S
Sbjct: 241 AKHSYSKASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNSS 300

Query: 329 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPL 388
           STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPI+PKISKQETETPL
Sbjct: 301 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIVPKISKQETETPL 360

Query: 389 QPSGSQPSKPVSRFKMQRR 407
           QPSGSQPSKPVSRFKMQRR
Sbjct: 361 QPSGSQPSKPVSRFKMQRR 379

BLAST of CmaCh20G002070 vs. ExPASy TrEMBL
Match: A0A6J1D6F4 (RNA polymerase II subunit 5-mediating protein homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018055 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.9e-146
Identity = 301/373 (80.70%), Postives = 331/373 (88.74%), Query Frame = 0

Query: 35  GGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEE 94
           GGAKGTV SLSSLFPA+DAHKAA RVQD +SEKQ+EL+ LRGFISDNANLINLVQKLPEE
Sbjct: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63

Query: 95  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 154
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123

Query: 155 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEA 214
           KAMMDDLKAEASFFDATASEAAEGLVEI EEYVEEN+SE+E  SGVNKQD  SV  VD+A
Sbjct: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEVDKA 183

Query: 215 RITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSK 274
           +ITEEDEEYARIM+RLDELE+EEELAA NDNQ DEE EEEK TSNQSLEK HD +  +SK
Sbjct: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243

Query: 275 ASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSSTQTRGM 334
            ST P QR+++IGS+E L KY QQQ+EAS +PSNC+  S QS ++ +LHGNNSSTQ   +
Sbjct: 244 TSTSPWQREKNIGSEEPLGKY-QQQEEASNDPSNCTGLSVQSLARDTLHGNNSSTQR--V 303

Query: 335 RNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ-PSGSQ 394
           RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS SQ
Sbjct: 304 RNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSSQ 363

Query: 395 PSKPVSRFKMQRR 407
           PSKPVSRFKMQRR
Sbjct: 364 PSKPVSRFKMQRR 373

BLAST of CmaCh20G002070 vs. ExPASy TrEMBL
Match: A0A6J1D7S6 (RNA polymerase II subunit 5-mediating protein homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018055 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.7e-145
Identity = 301/374 (80.48%), Postives = 331/374 (88.50%), Query Frame = 0

Query: 35  GGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEE 94
           GGAKGTV SLSSLFPA+DAHKAA RVQD +SEKQ+EL+ LRGFISDNANLINLVQKLPEE
Sbjct: 4   GGAKGTVTSLSSLFPAEDAHKAAARVQDTLSEKQKELDCLRGFISDNANLINLVQKLPEE 63

Query: 95  LHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSL 154
           L+HEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQ VEILKRRGKALDSQVDSL
Sbjct: 64  LYHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQAVEILKRRGKALDSQVDSL 123

Query: 155 KAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVDEA 214
           KAMMDDLKAEASFFDATASEAAEGLVEI EEYVEEN+SE+E  SGVNKQD  SV  VD+A
Sbjct: 124 KAMMDDLKAEASFFDATASEAAEGLVEIKEEYVEENSSEREPESGVNKQDVHSVPEVDKA 183

Query: 215 RITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSK 274
           +ITEEDEEYARIM+RLDELE+EEELAA NDNQ DEE EEEK TSNQSLEK HD +  +SK
Sbjct: 184 KITEEDEEYARIMARLDELEKEEELAAGNDNQGDEEDEEEKGTSNQSLEKFHDEELRFSK 243

Query: 275 ASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQS-SSKGSLHGNNSSTQTRG 334
            ST P QR+++IGS+E L KY QQQ+EAS +PSNC+  S QS + + +LHGNNSSTQ   
Sbjct: 244 TSTSPWQREKNIGSEEPLGKY-QQQEEASNDPSNCTGLSVQSLAREDTLHGNNSSTQR-- 303

Query: 335 MRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ-PSGS 394
           +RN +SAAKSVTFAE +EKNQTLP S+SEAFTGSI+ERPPI+ K SKQETETPLQ PS S
Sbjct: 304 VRNPSSAAKSVTFAEAREKNQTLPPSSSEAFTGSIIERPPIISKTSKQETETPLQMPSSS 363

Query: 395 QPSKPVSRFKMQRR 407
           QPSKPVSRFKMQRR
Sbjct: 364 QPSKPVSRFKMQRR 374

BLAST of CmaCh20G002070 vs. ExPASy TrEMBL
Match: A0A0A0KB27 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G127400 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.7e-142
Identity = 297/381 (77.95%), Postives = 328/381 (86.09%), Query Frame = 0

Query: 27  AGMIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLIN 86
           +G+IME  G  KGTV SL+SLFPAQDA KAA RVQDAISEKQRELE LRGFI+DN NLI 
Sbjct: 78  SGVIME-SGRVKGTVTSLASLFPAQDARKAASRVQDAISEKQRELEQLRGFITDNDNLIK 137

Query: 87  LVQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKA 146
           LVQKLPEELHHEVMVPFGKAAFFPGRL+HTNEFLVLLGEGYYAERTSKQT EILKRRGKA
Sbjct: 138 LVQKLPEELHHEVMVPFGKAAFFPGRLIHTNEFLVLLGEGYYAERTSKQTAEILKRRGKA 197

Query: 147 LDSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAP 206
           LDSQVDSLKAMMD+LKAEASFFDATASEA +GLVEI EEYVEEN  EQES SGVNKQD P
Sbjct: 198 LDSQVDSLKAMMDNLKAEASFFDATASEAEDGLVEIKEEYVEENFCEQESTSGVNKQDVP 257

Query: 207 SVSGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLH 266
           SVSGVD+A+I E D EYAR+M+R DELE+EEELAAAN N++D+E EEEK T NQSLE+ +
Sbjct: 258 SVSGVDKAKIAEIDAEYARMMARFDELEKEEELAAANGNKSDDEDEEEKGTQNQSLERFY 317

Query: 267 DAKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSL-HGN 326
             K S SK ST    RDE++ SKELL+KY Q+QQEASTNPSNCS  S Q+S K  +  GN
Sbjct: 318 GDKQSLSKGSTSTWPRDENVSSKELLNKY-QKQQEASTNPSNCSGLSVQTSPKEDVTSGN 377

Query: 327 NSSTQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETET 386
           NS T+++ + N N AAKSVTFAEVKEKNQTLP ST++AFTGSI+ERPPI+PK SKQETET
Sbjct: 378 NSLTESQRVINPNPAAKSVTFAEVKEKNQTLPPSTNQAFTGSIIERPPIIPKTSKQETET 437

Query: 387 PLQPSGSQPSKPVSRFKMQRR 407
            LQPSGSQPSKPVSRFKMQRR
Sbjct: 438 TLQPSGSQPSKPVSRFKMQRR 456

BLAST of CmaCh20G002070 vs. NCBI nr
Match: XP_022985987.1 (RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima] >XP_022985988.1 RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima] >XP_022985989.1 RNA polymerase II subunit 5-mediating protein homolog [Cucurbita maxima])

HSP 1 Score: 693.0 bits (1787), Expect = 1.6e-195
Identity = 378/378 (100.00%), Postives = 378/378 (100.00%), Query Frame = 0

Query: 29  MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV 88
           MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV
Sbjct: 1   MIMEGGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLV 60

Query: 89  QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD 148
           QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD
Sbjct: 61  QKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALD 120

Query: 149 SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV 208
           SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV
Sbjct: 121 SQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSV 180

Query: 209 SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA 268
           SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA
Sbjct: 181 SGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDA 240

Query: 269 KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS 328
           KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS
Sbjct: 241 KHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSS 300

Query: 329 TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ 388
           TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ
Sbjct: 301 TQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQ 360

Query: 389 PSGSQPSKPVSRFKMQRR 407
           PSGSQPSKPVSRFKMQRR
Sbjct: 361 PSGSQPSKPVSRFKMQRR 378

BLAST of CmaCh20G002070 vs. NCBI nr
Match: XP_023512548.1 (RNA polymerase II subunit 5-mediating protein homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675.2 bits (1741), Expect = 3.4e-190
Identity = 370/379 (97.63%), Postives = 377/379 (99.47%), Query Frame = 0

Query: 29  MIME-GGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINL 88
           MIME GGGGAKGTVA+LSSLFPAQDAHKAAMRVQDAIS+KQRELEHLRGFISDNANLINL
Sbjct: 1   MIMEGGGGGAKGTVAALSSLFPAQDAHKAAMRVQDAISDKQRELEHLRGFISDNANLINL 60

Query: 89  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 148
           VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL
Sbjct: 61  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 120

Query: 149 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPS 208
           DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEE+NSEQESGSGVNKQDAPS
Sbjct: 121 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGSGVNKQDAPS 180

Query: 209 VSGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHD 268
           VSGVDEARITEEDEEYARIMSRLDELE+EEELAAAN+NQNDEEGEEEKETSNQS+EKLHD
Sbjct: 181 VSGVDEARITEEDEEYARIMSRLDELEKEEELAAANNNQNDEEGEEEKETSNQSMEKLHD 240

Query: 269 AKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNS 328
           AKHSYSKAST PC+RDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNS
Sbjct: 241 AKHSYSKASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNS 300

Query: 329 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPL 388
           STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPL
Sbjct: 301 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPL 360

Query: 389 QPSGSQPSKPVSRFKMQRR 407
           QPSGSQPSKPVSRFKMQRR
Sbjct: 361 QPSGSQPSKPVSRFKMQRR 379

BLAST of CmaCh20G002070 vs. NCBI nr
Match: XP_022943608.1 (RNA polymerase II subunit 5-mediating protein homolog [Cucurbita moschata] >XP_022943609.1 RNA polymerase II subunit 5-mediating protein homolog [Cucurbita moschata])

HSP 1 Score: 669.5 bits (1726), Expect = 1.9e-188
Identity = 368/379 (97.10%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 29  MIME-GGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINL 88
           MIME GGGGAKGTVA+LSSLFPAQDAHKAAMRVQDAISEKQRELEHL GFISDNANLINL
Sbjct: 1   MIMEGGGGGAKGTVAALSSLFPAQDAHKAAMRVQDAISEKQRELEHLLGFISDNANLINL 60

Query: 89  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 148
           VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL
Sbjct: 61  VQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKAL 120

Query: 149 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPS 208
           DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEE+NSEQESGSGVNKQDAPS
Sbjct: 121 DSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGSGVNKQDAPS 180

Query: 209 VSGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHD 268
           VSGVDEARITEEDEEYARIMSRLDELE+EEELAAANDNQNDE+GEEE ETSNQSLEKLHD
Sbjct: 181 VSGVDEARITEEDEEYARIMSRLDELEKEEELAAANDNQNDEKGEEENETSNQSLEKLHD 240

Query: 269 AKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNS 328
           AKHSYSKAST PC+RDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGN+S
Sbjct: 241 AKHSYSKASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNSS 300

Query: 329 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPL 388
           STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPI+PKISKQETETPL
Sbjct: 301 STQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIVPKISKQETETPL 360

Query: 389 QPSGSQPSKPVSRFKMQRR 407
           QPSGSQPSKPVSRFKMQRR
Sbjct: 361 QPSGSQPSKPVSRFKMQRR 379

BLAST of CmaCh20G002070 vs. NCBI nr
Match: KAG6570522.1 (RNA polymerase II subunit 5-mediating protein-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.2 bits (1720), Expect = 9.3e-188
Identity = 364/374 (97.33%), Postives = 371/374 (99.20%), Query Frame = 0

Query: 33  GGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLP 92
           GGGGAKGTVA+LSSLFPAQDAHKAAMRVQDAISEKQRELEHL GFISDNANLINLVQKLP
Sbjct: 4   GGGGAKGTVAALSSLFPAQDAHKAAMRVQDAISEKQRELEHLLGFISDNANLINLVQKLP 63

Query: 93  EELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVD 152
           EELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVD
Sbjct: 64  EELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVD 123

Query: 153 SLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSGVD 212
           SLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEE+N+EQESGSGVNKQDAPSVSGVD
Sbjct: 124 SLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNNEQESGSGVNKQDAPSVSGVD 183

Query: 213 EARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSY 272
           EARITEEDEEYARIMSRLDELE+EEELAAANDNQNDEEGEEE ETSNQSLEKLHDAKHSY
Sbjct: 184 EARITEEDEEYARIMSRLDELEKEEELAAANDNQNDEEGEEENETSNQSLEKLHDAKHSY 243

Query: 273 SKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNNSSTQTR 332
           SKAST PC+RDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGN+SSTQTR
Sbjct: 244 SKASTSPCRRDEDIGSKELLDKYQQQQQEASTNPSNCSNFSEQSSSKGSLHGNSSSTQTR 303

Query: 333 GMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIMPKISKQETETPLQPSGS 392
           GMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPI+PKISKQETETPLQPSGS
Sbjct: 304 GMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVERPPIVPKISKQETETPLQPSGS 363

Query: 393 QPSKPVSRFKMQRR 407
           QPSKPVSRFKMQRR
Sbjct: 364 QPSKPVSRFKMQRR 377

BLAST of CmaCh20G002070 vs. NCBI nr
Match: KAG7010380.1 (RNA polymerase II subunit 5-mediating protein-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 635.2 bits (1637), Expect = 3.9e-178
Identity = 359/395 (90.89%), Postives = 367/395 (92.91%), Query Frame = 0

Query: 25  LAAGMIME-GGGGAKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNAN 84
           L +GMIME GGGGAKGTVA+LSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNAN
Sbjct: 56  LESGMIMEGGGGGAKGTVAALSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNAN 115

Query: 85  LINLVQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRR 144
           LINLVQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRR
Sbjct: 116 LINLVQKLPEELHHEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRR 175

Query: 145 GKALDSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQ 204
           GKALDSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEE+NSEQESGS     
Sbjct: 176 GKALDSQVDSLKAMMDDLKAEASFFDATASEAAEGLVEITEEYVEEDNSEQESGS----- 235

Query: 205 DAPSVSGVDEARITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLE 264
                  VDEARITEEDEEYARIMSRLDELE+EEELAAANDNQNDEEGEEEKETSNQSLE
Sbjct: 236 -------VDEARITEEDEEYARIMSRLDELEKEEELAAANDNQNDEEGEEEKETSNQSLE 295

Query: 265 KLHDAKHSYSKASTFPCQRDEDIGSKELLDKYQQQQQEASTNPSN------------CSN 324
           KLHDAKHSYSKAST PC+RDE+IGSKELLDKYQQQQQEASTNPSN            CSN
Sbjct: 296 KLHDAKHSYSKASTSPCRRDENIGSKELLDKYQQQQQEASTNPSNVTNLLFALLFITCSN 355

Query: 325 FSEQSSSKGSLHGNNSSTQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVER 384
           FSEQSSSKGSLHGN+SSTQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVER
Sbjct: 356 FSEQSSSKGSLHGNSSSTQTRGMRNTNSAAKSVTFAEVKEKNQTLPASTSEAFTGSIVER 415

Query: 385 PPIMPKISKQETETPLQPSGSQPSKPVSRFKMQRR 407
           PPI+PKISKQETETPLQPSG QPSKPVSRFKMQRR
Sbjct: 416 PPIVPKISKQETETPLQPSGPQPSKPVSRFKMQRR 438

BLAST of CmaCh20G002070 vs. TAIR 10
Match: AT1G03760.1 (Prefoldin chaperone subunit family protein )

HSP 1 Score: 204.9 bits (520), Expect = 1.2e-52
Identity = 160/402 (39.80%), Postives = 220/402 (54.73%), Query Frame = 0

Query: 37  AKGTVASLSSLFPAQDAHKAAMRVQDAISEKQRELEHLRGFISDNANLINLVQKLPEELH 96
           AKGTV  L   F  +D  KAA  +++ I EK+ E+  L+ ++ +N NLINLV+KLP++LH
Sbjct: 5   AKGTVTPLG--FSEEDVRKAASYMEEKIGEKRVEMNRLQQYVDENDNLINLVKKLPDQLH 64

Query: 97  HEVMVPFGKAAFFPGRLVHTNEFLVLLGEGYYAERTSKQTVEILKRRGKALDSQVDSLKA 156
           H VMVPFGK AFFPGRL+HTNE LVLLGE YY +R+SKQTV+ LKRR K L SQ+ SLKA
Sbjct: 65  HNVMVPFGKMAFFPGRLIHTNECLVLLGENYYTDRSSKQTVDFLKRRDKTLQSQIHSLKA 124

Query: 157 MMDDLKAEASFFDATASEAAEGLVEITEEYVEENNSEQESGSGVNKQDAPSVSG-VDEAR 216
            ++DL+ EASFF  TASE A+GLVEI EEYVEE++S         K+      G  +E  
Sbjct: 125 EIEDLQTEASFFTTTASEVADGLVEIREEYVEEDSSAPVVIHSSEKEPCNLSEGETEEGE 184

Query: 217 ITEEDEEYARIMSRLDELEEEEELAAANDNQNDEEGEEEKETSNQSLEKLHDAKHSYSKA 276
           +  ED+++ARIM+RL+ELE EEEL         E+G+   E  + S+E +   +H   K 
Sbjct: 185 L--EDDDFARIMARLNELEIEEEL-------EGEDGDSRGEDPDSSVESVEQIQHDLVKG 244

Query: 277 STFPCQRDE-DIGSKELLDKYQQQQQEASTNP---------------------------- 336
           S     R   + G +E       +    S++P                            
Sbjct: 245 SRGETDRGRVEYGKQETTISVPNKASGHSSSPRVSEPRVEAKIIQVLPETHPHKDLDDPL 304

Query: 337 SNCSNFSEQSSSKGSLHGNNSSTQTRGMRNTNSAAKSVTFAEVK-EKNQTLPA-STSEAF 396
           +     ++  S +G   G          ++  + AK+      K E  +  P   +++AF
Sbjct: 305 NRIRPMAQYLSKEGQSRGGTPQQNAETWKDFRTNAKTNVLGPQKIEPQKPEPEFDSTKAF 364

Query: 397 TGSIVERPPIMPKISKQETETPLQPSGSQPSKPVSRFKMQRR 407
           TGSIVE   I+   +  +T    Q S SQPSKPVSRF+ +RR
Sbjct: 365 TGSIVEHAHILETSAHSQT----QSSTSQPSKPVSRFRAKRR 391