Homology
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match:
Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)
HSP 1 Score: 878.6 bits (2269), Expect = 4.6e-254
Identity = 448/716 (62.57%), Postives = 548/716 (76.54%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
M D ++ D + + A+L + Q T EEL+ +++AP+K+E+L PPP
Sbjct: 1 MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60
Query: 61 SCNNVKDAASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
+ S +ND D AP S LV+EKKSKRQ+KRERR++ ST +LCP++A+T DV
Sbjct: 61 ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120
Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
SC+Y DKCRF+HDIEAFK+QK D++G+CPF + C YGL+CRF G+H++ G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180
Query: 181 VTLKKCSEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSN- 240
+K SEMN NK+ Q+LLWKNKM F ADAKLK LGLLGH K + +E + N
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240
Query: 241 -------DSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSS 300
+ D SE ++M D+DIP G ++E RP+KK KS
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300
Query: 301 ENCHSDEIDN--GGACMIDATIREGHTPIESEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
+N + ++ G + + T + G+ ++ V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360
Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480
Query: 481 IASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
++S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540
Query: 541 NRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
+C+ A +LQVIGNGDV+SY +WN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600
Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660
Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match:
Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)
HSP 1 Score: 858.6 bits (2217), Expect = 4.9e-248
Identity = 438/688 (63.66%), Postives = 527/688 (76.60%), Query Frame = 0
Query: 20 ASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGDDKAAPSS 79
+S +AS+ + +PEELVA+A+APVK +LRPP S KD GK +G +
Sbjct: 19 SSPAASSPPRPSPEELVARAVAPVKPAFLRPP---LSATPPKD---EGKANGG-----GA 78
Query: 80 LVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPAD 139
+V EKKSKRQ+KRER+QEQKS+SHLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPAD
Sbjct: 79 VVAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPAD 138
Query: 140 LDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQKLLWKNKM 199
L+G CPF+ +QLCPYGL CRF GTHK+ A +++ K E+N LNKD+QKLLWKNK
Sbjct: 139 LEGTCPFTNLDQLCPYGLTCRFLGTHKDIHAASGNLSEK--HEINALNKDIQKLLWKNKY 198
Query: 200 KFPKADAKLKELGLLGHGKSK-------SRVKEEKEDAVSNDSVADRTCCSEVNNDMDAD 259
KFPKA A++K LGL KSK +V + D ++D + C VN + D+
Sbjct: 199 KFPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDG-NDDENKEPLCNPPVNAECDST 258
Query: 260 LDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHTP 319
L EE ++EG D S P +P+ SDEID GA ++ T E P
Sbjct: 259 L------CEELDRSEGEPLIDNSIPCVEPRPTKKSKVESDEIDKHGAGTLN-TNTESEDP 318
Query: 320 IES---EVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGAD 379
S E N+S +D H REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD
Sbjct: 319 NLSNGLEPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGAD 378
Query: 380 VTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFI 439
+TCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D EC +DFI
Sbjct: 379 ITCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNECSVDFI 438
Query: 440 DINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSL 499
DINMGCPIDIVVNKGAGS+LL KPMR+KS+V+ AS + + P+T+KVRT +FEG+NR DS+
Sbjct: 439 DINMGCPIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSI 498
Query: 500 IADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRH 559
++DI +WGA A+T+HGR+RQQRYSKLADW+YI +C+ APD L V+GNGDVFS+ +WN+H
Sbjct: 499 VSDIYDWGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKH 558
Query: 560 KVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKG 619
K++T MIARGALIKPW+FTE+KEQR+WDITSGER NILKDFV FGLEHWGSD+KG
Sbjct: 559 VSGCSKISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKG 618
Query: 620 VETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKIS 679
VETTR+FLLEWLSYTCRYIPVGLLDV+PQRLNWRPPSY GRDDLETLM SDSAADWI+IS
Sbjct: 619 VETTRYFLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAADWIRIS 678
Query: 680 EMLLGKVPDGFSFAPKHKSNAYDRAENG 698
EMLLGKVP+GF+F PKHKSNAYDRAENG
Sbjct: 679 EMLLGKVPEGFTFTPKHKSNAYDRAENG 685
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match:
Q91XI1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)
HSP 1 Score: 520.0 bits (1338), Expect = 4.1e-146
Identity = 290/687 (42.21%), Postives = 391/687 (56.91%), Query Frame = 0
Query: 38 KAIAPVK----------REYLRPPPVRSSCNNVK--DAASTGK-----NDGDDKAAPSSL 97
+ +AP+K EYL +C V D A G DG +
Sbjct: 21 RGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLEDGQENGKTEVA 80
Query: 98 VKEKKSKRQMKRERRQEQKSTSH----------LCPEIAKTGDVSSCRYSDKCRFSHDIE 157
++ + +RQ+ + R + KS H LCP + + C + D+CRF HD+
Sbjct: 81 IESR--ERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQE-PATPCAFGDRCRFLHDVG 140
Query: 158 AFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGSSVTLKKCSEM----N 217
+ KPADL C CP+ + CRFAG H EG + +C+++ N
Sbjct: 141 RYLETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRN 200
Query: 218 GLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRTCCSE 277
GL++ +Q+ L K ++ F +A+ L L
Sbjct: 201 GLDRALQQQLRKRQVCFERAEQALNRL--------------------------------- 260
Query: 278 VNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDA 337
+P VPE + + PK S CH+ GGA
Sbjct: 261 ------TQSPMPTVVPE------------TTVAMATPKQNS---CHAQLDTVGGA----- 320
Query: 338 TIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCK 397
TP S V L D D ++ P EKK +D KLYLAPLTT GNLPFRR+CK
Sbjct: 321 -----GTPQSSPVPTCGPLTDEDV-IRLRPCEKKRLDISGKLYLAPLTTCGNLPFRRICK 380
Query: 398 VLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKEC 457
GADVTCGEMA+CTNLLQGQ SEWALL+RH ED+FGVQ+ GA+PDT+++ EL+++
Sbjct: 381 RFGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTKCAELLNRTI 440
Query: 458 VIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKN 517
+DF+DIN+GCPID+V KG G AL+ + + + +V +G++D P+T+K+RTG E +
Sbjct: 441 DVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNGVLDVPLTVKMRTGVQERVS 500
Query: 518 RIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA 577
L+ ++ +WG VT+HGR+R+QRY++LADW YI +C+ +A + + GNGD+ S+
Sbjct: 501 LAHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLFGNGDILSFE 560
Query: 578 EWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWG 637
+ N +A M+ARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+DF H+GLEHWG
Sbjct: 561 DAN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTHYGLEHWG 620
Query: 638 SDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAAD 692
SDT+GVE TR FLLEWLS+ CRY+PVGLL+ PQR+N RPP Y GRD LETLM+S AAD
Sbjct: 621 SDTQGVERTRRFLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLETLMASQQAAD 636
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match:
Q96G46 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)
HSP 1 Score: 517.7 bits (1332), Expect = 2.0e-145
Identity = 280/637 (43.96%), Postives = 376/637 (59.03%), Query Frame = 0
Query: 66 TGKNDGDDKAAPSSLVKEKKSKRQMKRER---RQEQKSTSHLCPEIAKTGDVSSCRYSDK 125
T ++ A P ++ +K R + R + + LCP + + + C + D+
Sbjct: 82 TADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQE-SAAKCFFGDR 141
Query: 126 CRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC-- 185
CRF HD+ + KPADL C CPYG+ CRFAG H P G ++ ++
Sbjct: 142 CRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLR--PEGQNLVQEELAA 201
Query: 186 ------SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSND 245
S NGL+K +Q+ L K +++F +A+ L+ R + A +
Sbjct: 202 RGTQPPSIRNGLDKALQQQLRKREVRFERAEQALR------------RFSQGPTPAAA-- 261
Query: 246 SVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEI 305
VP EGT + R ENC + ++
Sbjct: 262 ------------------------VP------EGTAAEGAPR---------QENCGAQQV 321
Query: 306 DNGGACMIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTV 365
G TP S V L D D ++ P EKK +D R KLYLAPLTT
Sbjct: 322 PAGPGT---------STPPSSPVRTCGPLTDEDV-VRLRPCEKKRLDIRGKLYLAPLTTC 381
Query: 366 GNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVS 425
GNLPFRR+CK GADVTCGEMA+CTNLLQGQ SEWALL+RH ED+FGVQ+ GA+PDT++
Sbjct: 382 GNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPDTMT 441
Query: 426 RAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIK 485
+ EL+ + +DF+DIN+GCPID+V KG G AL+ + + + +V + ++D P+T+K
Sbjct: 442 KCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPLTVK 501
Query: 486 VRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQV 545
+RTG E N L+ ++ +WG VT+HGR+R+QRY+KLADW+YI C A + +
Sbjct: 502 IRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQ-AASPMPL 561
Query: 546 IGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKD 605
GNGD+ S+ + NR + MIARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+D
Sbjct: 562 FGNGDILSFEDANR--AMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILRD 621
Query: 606 FVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLE 665
F ++GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y GRD LE
Sbjct: 622 FTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLE 649
Query: 666 TLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 692
TLM+S AADWI+ISEMLLG VP F+F PKHK+NAY
Sbjct: 682 TLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match:
Q3KRC5 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus3l PE=2 SV=1)
HSP 1 Score: 515.4 bits (1326), Expect = 1.0e-144
Identity = 280/642 (43.61%), Postives = 380/642 (59.19%), Query Frame = 0
Query: 60 VKDAASTGKN----DGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVS 119
++D GK + ++ P + KS+ MK +++ LCP + + +
Sbjct: 71 LEDGQENGKTEVAVESHERQVPKRARGQNKSRPHMKPAHYDKER----LCPSLLQE-SAT 130
Query: 120 SCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGS 179
C + D+CRF HD+ + KPADL C CPY + CRFAG H EG
Sbjct: 131 PCAFGDRCRFLHDVGRYLETKPADLGPHCVLFNTFGRCPYSMTCRFAGAHLGPEGQNLVQ 190
Query: 180 SVTLKKCSEM----NGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKED 239
+ +C+++ NGL++ +Q+ L K ++ F +A+ L L
Sbjct: 191 EEVVARCAQLPSVRNGLDRALQQQLRKRQVCFERAEQALSHL------------------ 250
Query: 240 AVSNDSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENC 299
+P PE T T + S +C
Sbjct: 251 ---------------------TQGPMPTIAPE---STVATLTPKHS------------SC 310
Query: 300 HSDEIDNGGACMIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLA 359
H ++DN G R+G S V L D D ++ P EKK +D KLYLA
Sbjct: 311 HV-QLDNVGG----DGARQG-----SPVPTCGPLTDEDV-VRLRPCEKKRLDISGKLYLA 370
Query: 360 PLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAY 419
PLTT GNLPFRR+CK GADVTCGEMAMCTNLLQGQ SEWALL+RH ED+FGVQ+ GA+
Sbjct: 371 PLTTCGNLPFRRICKRFGADVTCGEMAMCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAF 430
Query: 420 PDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDT 479
PDT+++ EL+++ +DF+DIN+GCPID+V KG G AL+ + + + +V + ++D
Sbjct: 431 PDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGMNEVLDV 490
Query: 480 PITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAP 539
P+T+K+RTG E + L+ ++ NWG VT+HGR+R+QRY++LADW YI +C+ +A
Sbjct: 491 PLTVKMRTGVQERVSLAHRLLPELRNWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA- 550
Query: 540 DSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERL 599
+ + GNGD+ S+ + N +A M+ARGAL+KPWLFTEIKEQR+WDI+S ERL
Sbjct: 551 SPMPLFGNGDILSFEDAN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERL 610
Query: 600 NILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFG 659
+IL+DF H+GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y G
Sbjct: 611 DILRDFTHYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLG 639
Query: 660 RDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 692
RD LETLM+S AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 671 RDYLETLMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 639
BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match:
A0A6J1HXQ8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC111468431 PE=3 SV=1)
HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 696/697 (99.86%), Postives = 696/697 (99.86%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match:
A0A6J1GL24 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LOC111455351 PE=3 SV=1)
HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/697 (97.27%), Postives = 686/697 (98.42%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYA+WNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match:
A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)
HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 630/697 (90.39%), Postives = 662/697 (94.98%), Query Frame = 0
Query: 2 EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
EDSV+A+L D TAVSE P S S+ G LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3 EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62
Query: 62 DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
DA ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63 DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181
KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
Query: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241
EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
Query: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 301
CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDN GAC
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GAC 302
Query: 302 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 361
MID TI EGH PIESE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 303 MIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 362
Query: 362 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 421
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 363 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 422
Query: 422 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 481
DKECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+
Sbjct: 423 DKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYY 482
Query: 482 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 541
EGKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 483 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 542
Query: 542 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 601
FSY +WN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGL
Sbjct: 543 FSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGL 602
Query: 602 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 661
EHWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 603 EHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSD 662
Query: 662 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match:
A0A5A7U7Y0 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00190 PE=3 SV=1)
HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 622/686 (90.67%), Postives = 651/686 (94.90%), Query Frame = 0
Query: 13 TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGD 72
TAVSE P S SA G TP++LVAKAIAPV ++LRPPP+RSS N +KDA ST NDGD
Sbjct: 7 TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66
Query: 73 DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67 DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126
Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQK 192
KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186
Query: 193 LLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRTCCSEVNNDMDA 252
LLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VADRTCCSE NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246
Query: 253 DLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHT 312
DL+IP+EVPEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGGACMID TI EGH
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGGACMIDETIGEGHA 306
Query: 313 PIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 372
PIESEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT
Sbjct: 307 PIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 366
Query: 373 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDI 432
CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDI
Sbjct: 367 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDI 426
Query: 433 NMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIA 492
NMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIA
Sbjct: 427 NMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIA 486
Query: 493 DIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKV 552
DIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA+WN+HK
Sbjct: 487 DIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKD 546
Query: 553 ESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVE 612
E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVE
Sbjct: 547 ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVE 606
Query: 613 TTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 672
TTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM
Sbjct: 607 TTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 666
Query: 673 LLGKVPDGFSFAPKHKSNAYDRAENG 698
LLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 667 LLGKVPDGFSFAPKHKSNAYDRAENG 692
BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match:
A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)
HSP 1 Score: 1264.2 bits (3270), Expect = 0.0e+00
Identity = 621/686 (90.52%), Postives = 650/686 (94.75%), Query Frame = 0
Query: 13 TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGD 72
TAVSE P S SA G TP++LVAKAIAPV ++LRPPP+RSS N +KDA ST NDGD
Sbjct: 7 TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66
Query: 73 DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67 DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126
Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQK 192
KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186
Query: 193 LLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRTCCSEVNNDMDA 252
LLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VADRTCCSE NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246
Query: 253 DLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHT 312
DL+IP+EVPEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDN GACMID TI EGH
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDN-GACMIDETIGEGHA 306
Query: 313 PIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 372
PIESEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT
Sbjct: 307 PIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 366
Query: 373 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDI 432
CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDI
Sbjct: 367 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDI 426
Query: 433 NMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIA 492
NMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIA
Sbjct: 427 NMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIA 486
Query: 493 DIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKV 552
DIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA+WN+HK
Sbjct: 487 DIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKD 546
Query: 553 ESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVE 612
E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVE
Sbjct: 547 ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVE 606
Query: 613 TTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 672
TTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM
Sbjct: 607 TTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 666
Query: 673 LLGKVPDGFSFAPKHKSNAYDRAENG 698
LLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 667 LLGKVPDGFSFAPKHKSNAYDRAENG 691
BLAST of CmaCh19G007050 vs. NCBI nr
Match:
XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])
HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 696/697 (99.86%), Postives = 696/697 (99.86%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. NCBI nr
Match:
KAG6572077.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011741.1 tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1376.7 bits (3562), Expect = 0.0e+00
Identity = 681/697 (97.70%), Postives = 687/697 (98.57%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. NCBI nr
Match:
XP_023554076.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1373.6 bits (3554), Expect = 0.0e+00
Identity = 682/697 (97.85%), Postives = 689/697 (98.85%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELR PTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSS NNV
Sbjct: 1 MEDSVSAELRVPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSGNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDAASTGKNDGD+KAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDNKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV+LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVSLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
CCSEVNNDMDADLDIP+EVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPVEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATI EGHTPIESEVVND VLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIESEVVNDIVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGA AVT+HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGASAVTVHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. NCBI nr
Match:
XP_022952746.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata])
HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/697 (97.27%), Postives = 686/697 (98.42%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60
Query: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300
Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480
Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
FSYA+WNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600
Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of CmaCh19G007050 vs. NCBI nr
Match:
XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])
HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 630/697 (90.39%), Postives = 662/697 (94.98%), Query Frame = 0
Query: 2 EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
EDSV+A+L D TAVSE P S S+ G LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3 EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62
Query: 62 DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
DA ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63 DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181
KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
Query: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241
EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
Query: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 301
CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDN GAC
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GAC 302
Query: 302 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 361
MID TI EGH PIESE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 303 MIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 362
Query: 362 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 421
RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 363 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 422
Query: 422 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 481
DKECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+
Sbjct: 423 DKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYY 482
Query: 482 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 541
EGKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 483 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 542
Query: 542 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 601
FSY +WN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGL
Sbjct: 543 FSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGL 602
Query: 602 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 661
EHWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 603 EHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSD 662
Query: 662 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
BLAST of CmaCh19G007050 vs. TAIR 10
Match:
AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )
HSP 1 Score: 878.6 bits (2269), Expect = 3.2e-255
Identity = 448/716 (62.57%), Postives = 548/716 (76.54%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
M D ++ D + + A+L + Q T EEL+ +++AP+K+E+L PPP
Sbjct: 1 MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60
Query: 61 SCNNVKDAASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
+ S +ND D AP S LV+EKKSKRQ+KRERR++ ST +LCP++A+T DV
Sbjct: 61 ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120
Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
SC+Y DKCRF+HDIEAFK+QK D++G+CPF + C YGL+CRF G+H++ G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180
Query: 181 VTLKKCSEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSN- 240
+K SEMN NK+ Q+LLWKNKM F ADAKLK LGLLGH K + +E + N
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240
Query: 241 -------DSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSS 300
+ D SE ++M D+DIP G ++E RP+KK KS
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300
Query: 301 ENCHSDEIDN--GGACMIDATIREGHTPIESEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
+N + ++ G + + T + G+ ++ V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360
Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480
Query: 481 IASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
++S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540
Query: 541 NRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
+C+ A +LQVIGNGDV+SY +WN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600
Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660
Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691
BLAST of CmaCh19G007050 vs. TAIR 10
Match:
AT3G49640.1 (Aldolase-type TIM barrel family protein )
HSP 1 Score: 107.1 bits (266), Expect = 5.9e-23
Identity = 85/309 (27.51%), Postives = 144/309 (46.60%), Query Frame = 0
Query: 340 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 399
+D+++KL LAP+ VG L FR + GAD+T GE + C L + +
Sbjct: 1 MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60
Query: 400 RHSSEDLFGVQICGAYPDTV---------SRAVELIDKEC-VIDFIDINMGCPIDIVVNK 459
+ + +F C V RA++ + C + IDINMGCP +
Sbjct: 61 KGTDNVVFST--CDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQG 120
Query: 460 GAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTI 519
G G+ALL KP + ++ +D P+T K+R + L I G A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180
Query: 520 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIAR 579
HGR R A W+ I +A S+ VI NGDV Y +++R K + A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIKT-ATGAASVMVAR 240
Query: 580 GALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLS- 632
GA+ +F+ K + +W+ ++ K ++ + W +D V++T++ + E ++
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290
BLAST of CmaCh19G007050 vs. TAIR 10
Match:
AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )
HSP 1 Score: 77.0 bits (188), Expect = 6.6e-14
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = 0
Query: 343 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 402
R K +AP+ LPFR +C+ GA M + T + + E+ +
Sbjct: 87 RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146
Query: 403 LFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSV 462
LF VQ C PDT+ A + ++ C D++DIN+GCP I G+ L+ +KS+
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206
Query: 463 VEIASGIVDTPITIKVRT-GYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSK--LA 522
VE + ++ P++ K+R E + ++ D G + +HGRTR ++ K A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266
Query: 523 DWEYINRCSSLAPDSLQVIGNGDV 541
DW I + + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9T0J6 | 4.6e-254 | 62.57 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=370... | [more] |
Q7XT07 | 4.9e-248 | 63.66 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonic... | [more] |
Q91XI1 | 4.1e-146 | 42.21 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Du... | [more] |
Q96G46 | 2.0e-145 | 43.96 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS... | [more] |
Q3KRC5 | 1.0e-144 | 43.61 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HXQ8 | 0.0e+00 | 99.86 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A6J1GL24 | 0.0e+00 | 97.27 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A0A0K4I1 | 0.0e+00 | 90.39 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7... | [more] |
A0A5A7U7Y0 | 0.0e+00 | 90.67 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... | [more] |
A0A5D3CK47 | 0.0e+00 | 90.52 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... | [more] |
Match Name | E-value | Identity | Description | |
XP_022969416.1 | 0.0e+00 | 99.86 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima] | [more] |
KAG6572077.1 | 0.0e+00 | 97.70 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita ar... | [more] |
XP_023554076.1 | 0.0e+00 | 97.85 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022952746.1 | 0.0e+00 | 97.27 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata] | [more] |
XP_004148548.1 | 0.0e+00 | 90.39 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 ... | [more] |
Match Name | E-value | Identity | Description | |
AT4G38890.1 | 3.2e-255 | 62.57 | FMN-linked oxidoreductases superfamily protein | [more] |
AT3G49640.1 | 5.9e-23 | 27.51 | Aldolase-type TIM barrel family protein | [more] |
AT5G67220.1 | 6.6e-14 | 28.43 | FMN-linked oxidoreductases superfamily protein | [more] |