CmaCh19G007050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh19G007050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptiontRNA-dihydrouridine(47) synthase [NAD(P)(+)]
LocationCma_Chr19: 7209539 .. 7214983 (+)
RNA-Seq ExpressionCmaCh19G007050
SyntenyCmaCh19G007050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGTTTCCAATCTTTTTCAGTTACTCGTGCGCCCTAGCGCAGCCTCCCACCCGCCCACTAATGGAAGACTCAGTTTCGGCTGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCAACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCTCCTGTTAAGCGCGAGTATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATGCTGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGTTCCTAGTCATTCATGTCGCTTTGTGTTTTTGTAGTGAAAGTTAGCATGCGTCATTTCTAGTTTTGGATTTTGCTTTCCTAAGCATTACTTGAAATCTAGTTGAAGTGCCTGAAACCTTTTTGAATTGAATGGAGTTTGGAATATAACGTTCCTATCTGAAATTGTTGGTTAACGATTGATTAAACACTATAAAACACTTGCAAACGATTGTGATGAGTTTCACTGCATGCAACTCGGTCTTTTTTCTTGTTGTTTGTATATTTCTGTTGATTCCATGAAGAAAAATGATTCTAAGTATACGTTATAGGCTCTTCGATAATTTCCAGATTATGAATAGCAGATGTACTCTGTACAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGGTAGTGCATTTAAAATCCCACTTATAGCCCCCGTTTTCTTTATCGAACAAATTTATTTGTGAGTTTACCTTTATTTTGACAAGAGGTTTATGATCTCAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCGTGTAGATTTGCAGGTACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCAGTTACCTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATGCCAAACTCAAAGAACTTGGGCTCTTGGTATGACCAGAAAGCATACTTTGTGTTTTTTCTGAGAGCTTTATCTTTGTCATGGGACGTGACTCAGTATTTTTATTTCTCCTCGCATGCCTGTATATCACTTTGTTTTGACTAGATAAGGGTTGAAGAGGCTACAGCTATCAAGGAATAGACTCTAATGCATTTCTTAATCTATTACCCATAATGTTATCTGCTTCCATCGAATAATAAGACATTGACGTAAGTTTGGCGTTGTTAGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGCTGTATCAAATGATAGTGTTGCAGACAGAACATGCTGTAGCGAAGTGAATAATGACATGGATGCGGACTTAGATATCCCTTTGGAAGTTCCAGAAGAAAATGGGCAGACCGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATGAAATTGACAATGGTGGTTTATATTTTATTACTTTATCTTATAGTTTCATCTTTCTCTTGTTATTTATATCTCTCATGTGTGCAACAGGTGCATGTATGATTGATGCAACTATTAGAGAGGGCCACACACCAATTGAGTCTGAAGTTGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGTTGTGCAGCTTTCCCTTGGCTCTGCAGAAATTAATATTAATAAGGGGTTATTAATTGATTGACATCATCTAGCTATCAAGTTCCAAATTATAGGTTTTGAAAGTTATTTGTTTGTTCTTATGCAAACAAATAACTATTTAGCTATTTAGTAATTATGATGCTATGCAACTACATTTTCAGTTAGAGATAAGTATGCAGTTATATATTCTCTAACATGGTTTGGCCCTTTTCATTTTAATCTAAAATGTCATTGTTCATTATTTGGCATGATTCTCATTCATTCACTTGTTAATGATGTTTCTATTTCATATTTCTTAAGATGAGTTAAATTAAACACTGTTTTATATTATTGTCCTGCGTGGTATAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATTGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAATCGCTTCCGGAATTGTGGATACGCCCATAACAATTAAGGTATTGATTATACTTGGGTACTTGTTTCAATGGAACGGCCAAAATTAACTGTTGAAGTTGCTTCTAACTGAGGTTATGGGCACTCTCCTTACGTTTCTCTTTTTCTTTGTTTGTTTTGTGAGTTTTTTATTGCCCTAAATTTGTTACATGAATTGGTGATGGTCTTGTGGGAAGGAGGGAAAAGTTAAATGAAATGAGACGAATGAATACTTTTGGAGAGTTTAAGAGGCTAAAAGCTCAAAGGTTGTAATTAGGTGGATGTAGCATATACGAGTTGTTTTCTTATTTTGCATTCATAAGATTGAATTCTAGGAATTGAGTTGAAGATCAACAAATGCCCTGAACCCCTTGATTTTTGGATAGCTGTCTCCTGTATTAGTTTACATGAGGATAGTAAAAGGTGGTTTCTGGATAGCTCCCTCGAGGAGGAGCCCCTTCATAGCTCCCTCCAAGTGTTTGAAAGATGAGAGATTTCAAGCAGCTAATTGTTTTCGGTCGCTTGTTCTGGGAGGTAGTAATACAAATAGTTGTTGCCAAAAAATTGTGAGAGACCAGTTTTTTTTTGCCCCTCTTTTTTTTTTTTTTTTTTTTTTTTTTAACTCTTTATTTGNTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACTCTTTATTTGTGAAGCTGCTCTTCCAGCTGGGTTTTTGGTCTTTCATGATTTCCCATGAGGGAAGTTTTGATTCTTCGATCGTTTGATAACTATTGGACTTTTGGTTTTTGAAACATGTGCTTCTATATATAAAAATAGTGCTTGCTTCCTTGTAACTTCTTTACCATAGTCTTCATTTTTTAAATTATGAATTTTTGGCCAAATTCCAAAAAACAAAAATAAGTTTTTAAAACAATTTTTGCTTGGTTTTTGAAAATACTCCTAAAAAGTAGATAGCAAGACAAAGAAATCAACTAGTGTAGGTAGTGTTTATAAGTTAACTTTCAAAAACCAAATGACCGACCCAAAAAACAAAAACTGCAACAGCTCGAGAACTCGAGATCTAATTTCAATTGTGTATATTTGGGAGCATAATATTGAACCAGCATGTTCTTTCTTAGGGGGAGGGGGATTGAGTGTGGGGAAAGGGAACTGTTACTTGATAATCTATATATTTGTTATGTTCTTGGTTTCTCAAGTTTTTCAATTTGTACCATTGTGTTTTTCTTGCACTAGGTTCGGACAGGCTACTTTGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCCGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATTAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGAATGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGGTTTTTTTTGTTTTTTTTGCAGTTTGATGTTCTTTTTATTTGATTCTTCAACTCATTGAAGCTTATGTTGCTATCATCTGTTGCCCATTGCAGCCATGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATTACTTCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGTACATTTTGGACTTGAACATTGGGGTTCCGACACAAAAGGTATGTCGCTCGTTGCCTGTTGCTTGTCATACTGCTCGTTGCATTTCGATGTTACTACTGGTGAACACAACTCTTTGTGGAAAACATTCATACTCTTTATTGACACCAATCGAGAAGAGAATACTCTATAGAAACTACTACGCTCTATGCTCTTTGCTTTGTGCTTCTTTTGGGATAAAAACCTAGGTAGTGTGTGCTTTGTATTTCTTTTGGGATAAAAACCGTGGTAGGGTGGAGGAGGGGTATTGCTAGACAATCTATTCCTAGAATATCTCAATCTTTTTCTTACCTAAAATATTTAGATATTTTTATTTCTAGAATACTTAAATCATTTTTCTTGAGTATACAATGAGCTTAGGTAATTATTGGGCTTGACCCTATTGGCTAGTGATGATATTTTAACAGCTACCGTGCTTCTTCACTTGATTTTTATGCTATCCTTTCTTCCCTTTGTTAAATCTGCAGGTGTTGAGACGACAAGGCATTTCTTACTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCACCGTCATATTTTGGCCGTGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGGTAATACTTTTTTTACTTTTTAGTTCAAAAAGTGCTCAAACCAATCGACCTCGAATGGTAATCCCTTCCATTGTTACAGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGGCTTCTCGTTCGCACCCAAACATAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGATTTGGATTCATCACTGTAAGAGACGATCCGATCACTGAAATTCAAATTCGTTCTTCTGGTGCACACATAAACTGAGACACTTTCCATTGCCATTGGCAGAGTATTGTAAGGCCTTGGCTGGTTGAAGAGCTAATTTAGCTATTTTCACAAATTATTTATGTATTAATGTTCCAATTACATGATAAATCAGGTGCTCTACGCAGCTGTAAATTTATTTATTAATTATTATACCATGCTGACTACCTGGAAGGTCCTTCTGTTTAAGGTATTGTTGAGGAAATTCAAGTTTTACTTTGCTTATATTTATTTTTTATTTTAAT

mRNA sequence

CTTTGTTTCCAATCTTTTTCAGTTACTCGTGCGCCCTAGCGCAGCCTCCCACCCGCCCACTAATGGAAGACTCAGTTTCGGCTGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCAACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCTCCTGTTAAGCGCGAGTATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATGCTGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCGTGTAGATTTGCAGGTACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCAGTTACCTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATGCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGCTGTATCAAATGATAGTGTTGCAGACAGAACATGCTGTAGCGAAGTGAATAATGACATGGATGCGGACTTAGATATCCCTTTGGAAGTTCCAGAAGAAAATGGGCAGACCGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATGAAATTGACAATGGTGGTGCATGTATGATTGATGCAACTATTAGAGAGGGCCACACACCAATTGAGTCTGAAGTTGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATTGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAATCGCTTCCGGAATTGTGGATACGCCCATAACAATTAAGGTTCGGACAGGCTACTTTGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCCGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATTAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGAATGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGCCATGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATTACTTCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGTACATTTTGGACTTGAACATTGGGGTTCCGACACAAAAGGTGTTGAGACGACAAGGCATTTCTTACTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCACCGTCATATTTTGGCCGTGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGGCTTCTCGTTCGCACCCAAACATAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGATTTGGATTCATCACTGTAAGAGACGATCCGATCACTGAAATTCAAATTCGTTCTTCTGGTGCACACATAAACTGAGACACTTTCCATTGCCATTGGCAGAGTATTGTAAGGCCTTGGCTGGTTGAAGAGCTAATTTAGCTATTTTCACAAATTATTTATGTATTAATGTTCCAATTACATGATAAATCAGGTGCTCTACGCAGCTGTAAATTTATTTATTAATTATTATACCATGCTGACTACCTGGAAGGTCCTTCTGTTTAAGGTATTGTTGAGGAAATTCAAGTTTTACTTTGCTTATATTTATTTTTTATTTTAAT

Coding sequence (CDS)

ATGGAAGACTCAGTTTCGGCTGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCAACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCTCCTGTTAAGCGCGAGTATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATGCTGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCGTGTAGATTTGCAGGTACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCAGTTACCTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATGCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGCTGTATCAAATGATAGTGTTGCAGACAGAACATGCTGTAGCGAAGTGAATAATGACATGGATGCGGACTTAGATATCCCTTTGGAAGTTCCAGAAGAAAATGGGCAGACCGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATGAAATTGACAATGGTGGTGCATGTATGATTGATGCAACTATTAGAGAGGGCCACACACCAATTGAGTCTGAAGTTGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATTGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAATCGCTTCCGGAATTGTGGATACGCCCATAACAATTAAGGTTCGGACAGGCTACTTTGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCCGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATTAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGAATGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGCCATGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATTACTTCAGGGGAGCGGCTAAACATCTTGAAGGATTTTGTACATTTTGGACTTGAACATTGGGGTTCCGACACAAAAGGTGTTGAGACGACAAGGCATTTCTTACTGGAATGGCTTAGCTACACATGTAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCACCGTCATATTTTGGCCGTGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGGCTTCTCGTTCGCACCCAAACATAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGA

Protein sequence

MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Homology
BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match: Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)

HSP 1 Score: 878.6 bits (2269), Expect = 4.6e-254
Identity = 448/716 (62.57%), Postives = 548/716 (76.54%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
           M D ++    D +   +  A+L   +  Q      T EEL+ +++AP+K+E+L PPP   
Sbjct: 1   MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60

Query: 61  SCNNVKDAASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
                  + S  +ND  D  AP S LV+EKKSKRQ+KRERR++  ST +LCP++A+T DV
Sbjct: 61  ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120

Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
            SC+Y DKCRF+HDIEAFK+QK  D++G+CPF  +   C YGL+CRF G+H++    G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180

Query: 181 VTLKKCSEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSN- 240
              +K SEMN  NK+ Q+LLWKNKM F  ADAKLK LGLLGH K  +  +E   +   N 
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240

Query: 241 -------DSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSS 300
                  +   D    SE  ++M  D+DIP           G   ++E RP+KK KS   
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300

Query: 301 ENCHSDEIDN--GGACMIDATIREGHTPIESEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
           +N  + ++     G  + + T + G+   ++ V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360

Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
           FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420

Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
           GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480

Query: 481 IASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
           ++S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540

Query: 541 NRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
            +C+  A  +LQVIGNGDV+SY +WN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600

Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
           DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660

Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match: Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)

HSP 1 Score: 858.6 bits (2217), Expect = 4.9e-248
Identity = 438/688 (63.66%), Postives = 527/688 (76.60%), Query Frame = 0

Query: 20  ASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGDDKAAPSS 79
           +S +AS+  + +PEELVA+A+APVK  +LRPP    S    KD    GK +G       +
Sbjct: 19  SSPAASSPPRPSPEELVARAVAPVKPAFLRPP---LSATPPKD---EGKANGG-----GA 78

Query: 80  LVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPAD 139
           +V EKKSKRQ+KRER+QEQKS+SHLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPAD
Sbjct: 79  VVAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPAD 138

Query: 140 LDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQKLLWKNKM 199
           L+G CPF+  +QLCPYGL CRF GTHK+   A  +++ K   E+N LNKD+QKLLWKNK 
Sbjct: 139 LEGTCPFTNLDQLCPYGLTCRFLGTHKDIHAASGNLSEK--HEINALNKDIQKLLWKNKY 198

Query: 200 KFPKADAKLKELGLLGHGKSK-------SRVKEEKEDAVSNDSVADRTCCSEVNNDMDAD 259
           KFPKA A++K LGL    KSK        +V  +  D  ++D   +  C   VN + D+ 
Sbjct: 199 KFPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDG-NDDENKEPLCNPPVNAECDST 258

Query: 260 LDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHTP 319
           L       EE  ++EG    D S P  +P+        SDEID  GA  ++ T  E   P
Sbjct: 259 L------CEELDRSEGEPLIDNSIPCVEPRPTKKSKVESDEIDKHGAGTLN-TNTESEDP 318

Query: 320 IES---EVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGAD 379
             S   E  N+S    +D     H REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD
Sbjct: 319 NLSNGLEPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGAD 378

Query: 380 VTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFI 439
           +TCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D EC +DFI
Sbjct: 379 ITCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNECSVDFI 438

Query: 440 DINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSL 499
           DINMGCPIDIVVNKGAGS+LL KPMR+KS+V+ AS + + P+T+KVRT +FEG+NR DS+
Sbjct: 439 DINMGCPIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSI 498

Query: 500 IADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRH 559
           ++DI +WGA A+T+HGR+RQQRYSKLADW+YI +C+  APD L V+GNGDVFS+ +WN+H
Sbjct: 499 VSDIYDWGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKH 558

Query: 560 KVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKG 619
                K++T MIARGALIKPW+FTE+KEQR+WDITSGER NILKDFV FGLEHWGSD+KG
Sbjct: 559 VSGCSKISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKG 618

Query: 620 VETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKIS 679
           VETTR+FLLEWLSYTCRYIPVGLLDV+PQRLNWRPPSY GRDDLETLM SDSAADWI+IS
Sbjct: 619 VETTRYFLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAADWIRIS 678

Query: 680 EMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           EMLLGKVP+GF+F PKHKSNAYDRAENG
Sbjct: 679 EMLLGKVPEGFTFTPKHKSNAYDRAENG 685

BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match: Q91XI1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 4.1e-146
Identity = 290/687 (42.21%), Postives = 391/687 (56.91%), Query Frame = 0

Query: 38  KAIAPVK----------REYLRPPPVRSSCNNVK--DAASTGK-----NDGDDKAAPSSL 97
           + +AP+K           EYL       +C  V   D A  G       DG +       
Sbjct: 21  RGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLEDGQENGKTEVA 80

Query: 98  VKEKKSKRQMKRERRQEQKSTSH----------LCPEIAKTGDVSSCRYSDKCRFSHDIE 157
           ++ +  +RQ+ +  R + KS  H          LCP   +    + C + D+CRF HD+ 
Sbjct: 81  IESR--ERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQE-PATPCAFGDRCRFLHDVG 140

Query: 158 AFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGSSVTLKKCSEM----N 217
            +   KPADL   C        CP+ + CRFAG H   EG        + +C+++    N
Sbjct: 141 RYLETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRN 200

Query: 218 GLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRTCCSE 277
           GL++ +Q+ L K ++ F +A+  L  L                                 
Sbjct: 201 GLDRALQQQLRKRQVCFERAEQALNRL--------------------------------- 260

Query: 278 VNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDA 337
                     +P  VPE             +  +  PK  S   CH+     GGA     
Sbjct: 261 ------TQSPMPTVVPE------------TTVAMATPKQNS---CHAQLDTVGGA----- 320

Query: 338 TIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCK 397
                 TP  S V     L D D  ++  P EKK +D   KLYLAPLTT GNLPFRR+CK
Sbjct: 321 -----GTPQSSPVPTCGPLTDEDV-IRLRPCEKKRLDISGKLYLAPLTTCGNLPFRRICK 380

Query: 398 VLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKEC 457
             GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT+++  EL+++  
Sbjct: 381 RFGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTKCAELLNRTI 440

Query: 458 VIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKN 517
            +DF+DIN+GCPID+V  KG G AL+ +  + + +V   +G++D P+T+K+RTG  E  +
Sbjct: 441 DVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNGVLDVPLTVKMRTGVQERVS 500

Query: 518 RIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA 577
               L+ ++ +WG   VT+HGR+R+QRY++LADW YI +C+ +A   + + GNGD+ S+ 
Sbjct: 501 LAHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLFGNGDILSFE 560

Query: 578 EWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWG 637
           + N        +A  M+ARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+DF H+GLEHWG
Sbjct: 561 DAN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTHYGLEHWG 620

Query: 638 SDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAAD 692
           SDT+GVE TR FLLEWLS+ CRY+PVGLL+  PQR+N RPP Y GRD LETLM+S  AAD
Sbjct: 621 SDTQGVERTRRFLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLETLMASQQAAD 636

BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match: Q96G46 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)

HSP 1 Score: 517.7 bits (1332), Expect = 2.0e-145
Identity = 280/637 (43.96%), Postives = 376/637 (59.03%), Query Frame = 0

Query: 66  TGKNDGDDKAAPSSLVKEKKSKRQMKRER---RQEQKSTSHLCPEIAKTGDVSSCRYSDK 125
           T     ++ A P   ++ +K  R   + R   +      + LCP + +    + C + D+
Sbjct: 82  TADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQE-SAAKCFFGDR 141

Query: 126 CRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC-- 185
           CRF HD+  +   KPADL   C        CPYG+ CRFAG H    P G ++  ++   
Sbjct: 142 CRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLR--PEGQNLVQEELAA 201

Query: 186 ------SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSND 245
                 S  NGL+K +Q+ L K +++F +A+  L+            R  +    A +  
Sbjct: 202 RGTQPPSIRNGLDKALQQQLRKREVRFERAEQALR------------RFSQGPTPAAA-- 261

Query: 246 SVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEI 305
                                   VP      EGT +    R          ENC + ++
Sbjct: 262 ------------------------VP------EGTAAEGAPR---------QENCGAQQV 321

Query: 306 DNGGACMIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTV 365
             G             TP  S V     L D D  ++  P EKK +D R KLYLAPLTT 
Sbjct: 322 PAGPGT---------STPPSSPVRTCGPLTDEDV-VRLRPCEKKRLDIRGKLYLAPLTTC 381

Query: 366 GNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVS 425
           GNLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT++
Sbjct: 382 GNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPDTMT 441

Query: 426 RAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIK 485
           +  EL+ +   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   + ++D P+T+K
Sbjct: 442 KCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPLTVK 501

Query: 486 VRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQV 545
           +RTG  E  N    L+ ++ +WG   VT+HGR+R+QRY+KLADW+YI  C   A   + +
Sbjct: 502 IRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQ-AASPMPL 561

Query: 546 IGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKD 605
            GNGD+ S+ + NR       +   MIARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+D
Sbjct: 562 FGNGDILSFEDANR--AMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILRD 621

Query: 606 FVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLE 665
           F ++GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y GRD LE
Sbjct: 622 FTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLE 649

Query: 666 TLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 692
           TLM+S  AADWI+ISEMLLG VP  F+F PKHK+NAY
Sbjct: 682 TLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649

BLAST of CmaCh19G007050 vs. ExPASy Swiss-Prot
Match: Q3KRC5 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus3l PE=2 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.0e-144
Identity = 280/642 (43.61%), Postives = 380/642 (59.19%), Query Frame = 0

Query: 60  VKDAASTGKN----DGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVS 119
           ++D    GK     +  ++  P     + KS+  MK     +++    LCP + +    +
Sbjct: 71  LEDGQENGKTEVAVESHERQVPKRARGQNKSRPHMKPAHYDKER----LCPSLLQE-SAT 130

Query: 120 SCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGS 179
            C + D+CRF HD+  +   KPADL   C        CPY + CRFAG H   EG     
Sbjct: 131 PCAFGDRCRFLHDVGRYLETKPADLGPHCVLFNTFGRCPYSMTCRFAGAHLGPEGQNLVQ 190

Query: 180 SVTLKKCSEM----NGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKED 239
              + +C+++    NGL++ +Q+ L K ++ F +A+  L  L                  
Sbjct: 191 EEVVARCAQLPSVRNGLDRALQQQLRKRQVCFERAEQALSHL------------------ 250

Query: 240 AVSNDSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENC 299
                                    +P   PE    T  T +   S            +C
Sbjct: 251 ---------------------TQGPMPTIAPE---STVATLTPKHS------------SC 310

Query: 300 HSDEIDNGGACMIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLA 359
           H  ++DN G        R+G     S V     L D D  ++  P EKK +D   KLYLA
Sbjct: 311 HV-QLDNVGG----DGARQG-----SPVPTCGPLTDEDV-VRLRPCEKKRLDISGKLYLA 370

Query: 360 PLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAY 419
           PLTT GNLPFRR+CK  GADVTCGEMAMCTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+
Sbjct: 371 PLTTCGNLPFRRICKRFGADVTCGEMAMCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAF 430

Query: 420 PDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDT 479
           PDT+++  EL+++   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   + ++D 
Sbjct: 431 PDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGMNEVLDV 490

Query: 480 PITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAP 539
           P+T+K+RTG  E  +    L+ ++ NWG   VT+HGR+R+QRY++LADW YI +C+ +A 
Sbjct: 491 PLTVKMRTGVQERVSLAHRLLPELRNWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA- 550

Query: 540 DSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERL 599
             + + GNGD+ S+ + N        +A  M+ARGAL+KPWLFTEIKEQR+WDI+S ERL
Sbjct: 551 SPMPLFGNGDILSFEDAN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERL 610

Query: 600 NILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFG 659
           +IL+DF H+GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y G
Sbjct: 611 DILRDFTHYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLG 639

Query: 660 RDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 692
           RD LETLM+S  AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 671 RDYLETLMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 639

BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match: A0A6J1HXQ8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC111468431 PE=3 SV=1)

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 696/697 (99.86%), Postives = 696/697 (99.86%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
           CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match: A0A6J1GL24 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LOC111455351 PE=3 SV=1)

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/697 (97.27%), Postives = 686/697 (98.42%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
            C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYA+WNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match: A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)

HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 630/697 (90.39%), Postives = 662/697 (94.98%), Query Frame = 0

Query: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62

Query: 62  DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
           DA ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63  DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122

Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182

Query: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR 
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242

Query: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 301
           CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDN GAC
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GAC 302

Query: 302 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 361
           MID TI EGH PIESE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 303 MIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 362

Query: 362 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 421
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 363 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 422

Query: 422 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 481
           DKECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+
Sbjct: 423 DKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYY 482

Query: 482 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 541
           EGKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 483 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 542

Query: 542 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 601
           FSY +WN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGL
Sbjct: 543 FSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGL 602

Query: 602 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 661
           EHWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 603 EHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSD 662

Query: 662 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match: A0A5A7U7Y0 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00190 PE=3 SV=1)

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 622/686 (90.67%), Postives = 651/686 (94.90%), Query Frame = 0

Query: 13  TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGD 72
           TAVSE P S SA  G   TP++LVAKAIAPV  ++LRPPP+RSS N +KDA ST  NDGD
Sbjct: 7   TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66

Query: 73  DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
           DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67  DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126

Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQK 192
           KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186

Query: 193 LLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRTCCSEVNNDMDA 252
           LLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VADRTCCSE NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246

Query: 253 DLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHT 312
           DL+IP+EVPEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGGACMID TI EGH 
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGGACMIDETIGEGHA 306

Query: 313 PIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 372
           PIESEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT
Sbjct: 307 PIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 366

Query: 373 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDI 432
           CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDI
Sbjct: 367 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDI 426

Query: 433 NMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIA 492
           NMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIA
Sbjct: 427 NMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIA 486

Query: 493 DIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKV 552
           DIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA+WN+HK 
Sbjct: 487 DIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKD 546

Query: 553 ESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVE 612
           E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVE
Sbjct: 547 ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVE 606

Query: 613 TTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 672
           TTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM
Sbjct: 607 TTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 666

Query: 673 LLGKVPDGFSFAPKHKSNAYDRAENG 698
           LLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 667 LLGKVPDGFSFAPKHKSNAYDRAENG 692

BLAST of CmaCh19G007050 vs. ExPASy TrEMBL
Match: A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)

HSP 1 Score: 1264.2 bits (3270), Expect = 0.0e+00
Identity = 621/686 (90.52%), Postives = 650/686 (94.75%), Query Frame = 0

Query: 13  TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDAASTGKNDGD 72
           TAVSE P S SA  G   TP++LVAKAIAPV  ++LRPPP+RSS N +KDA ST  NDGD
Sbjct: 7   TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66

Query: 73  DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
           DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67  DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126

Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCSEMNGLNKDVQK 192
           KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186

Query: 193 LLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRTCCSEVNNDMDA 252
           LLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VADRTCCSE NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246

Query: 253 DLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGACMIDATIREGHT 312
           DL+IP+EVPEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDN GACMID TI EGH 
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDN-GACMIDETIGEGHA 306

Query: 313 PIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 372
           PIESEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT
Sbjct: 307 PIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVT 366

Query: 373 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDI 432
           CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDI
Sbjct: 367 CGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDI 426

Query: 433 NMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIA 492
           NMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIA
Sbjct: 427 NMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIA 486

Query: 493 DIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKV 552
           DIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYA+WN+HK 
Sbjct: 487 DIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKD 546

Query: 553 ESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVE 612
           E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVE
Sbjct: 547 ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVE 606

Query: 613 TTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 672
           TTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM
Sbjct: 607 TTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEM 666

Query: 673 LLGKVPDGFSFAPKHKSNAYDRAENG 698
           LLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 667 LLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of CmaCh19G007050 vs. NCBI nr
Match: XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 1402.5 bits (3629), Expect = 0.0e+00
Identity = 696/697 (99.86%), Postives = 696/697 (99.86%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
           CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. NCBI nr
Match: KAG6572077.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011741.1 tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1376.7 bits (3562), Expect = 0.0e+00
Identity = 681/697 (97.70%), Postives = 687/697 (98.57%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
            C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. NCBI nr
Match: XP_023554076.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1373.6 bits (3554), Expect = 0.0e+00
Identity = 682/697 (97.85%), Postives = 689/697 (98.85%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELR PTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSS NNV
Sbjct: 1   MEDSVSAELRVPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSGNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDAASTGKNDGD+KAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDNKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV+LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVSLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
           CCSEVNNDMDADLDIP+EVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 CCSEVNNDMDADLDIPVEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATI EGHTPIESEVVND VLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIESEVVNDIVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGA AVT+HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGASAVTVHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. NCBI nr
Match: XP_022952746.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/697 (97.27%), Postives = 686/697 (98.42%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60

Query: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 300
            C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDN GAC
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDN-GAC 300

Query: 301 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360
           MIDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 301 MIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 360

Query: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 361 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 420

Query: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 480
           DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYF
Sbjct: 421 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYF 480

Query: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540
           EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 481 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 540

Query: 541 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 600
           FSYA+WNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGL
Sbjct: 541 FSYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGL 600

Query: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660
           EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 601 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 660

Query: 661 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 SAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of CmaCh19G007050 vs. NCBI nr
Match: XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])

HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 630/697 (90.39%), Postives = 662/697 (94.98%), Query Frame = 0

Query: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62

Query: 62  DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
           DA ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63  DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122

Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182

Query: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE++E+AVSNDS VADR 
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242

Query: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGGAC 301
           CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDN GAC
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GAC 302

Query: 302 MIDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFR 361
           MID TI EGH PIESE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 303 MIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 362

Query: 362 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 421
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 363 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 422

Query: 422 DKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYF 481
           DKECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+
Sbjct: 423 DKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYY 482

Query: 482 EGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 541
           EGKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV
Sbjct: 483 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 542

Query: 542 FSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 601
           FSY +WN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGL
Sbjct: 543 FSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGL 602

Query: 602 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 661
           EHWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 603 EHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSD 662

Query: 662 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of CmaCh19G007050 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 878.6 bits (2269), Expect = 3.2e-255
Identity = 448/716 (62.57%), Postives = 548/716 (76.54%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
           M D ++    D +   +  A+L   +  Q      T EEL+ +++AP+K+E+L PPP   
Sbjct: 1   MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60

Query: 61  SCNNVKDAASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
                  + S  +ND  D  AP S LV+EKKSKRQ+KRERR++  ST +LCP++A+T DV
Sbjct: 61  ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120

Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
            SC+Y DKCRF+HDIEAFK+QK  D++G+CPF  +   C YGL+CRF G+H++    G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180

Query: 181 VTLKKCSEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSN- 240
              +K SEMN  NK+ Q+LLWKNKM F  ADAKLK LGLLGH K  +  +E   +   N 
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240

Query: 241 -------DSVADRTCCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSS 300
                  +   D    SE  ++M  D+DIP           G   ++E RP+KK KS   
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300

Query: 301 ENCHSDEIDN--GGACMIDATIREGHTPIESEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
           +N  + ++     G  + + T + G+   ++ V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360

Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
           FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420

Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
           GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480

Query: 481 IASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
           ++S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540

Query: 541 NRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
            +C+  A  +LQVIGNGDV+SY +WN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600

Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
           DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660

Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
           WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of CmaCh19G007050 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 107.1 bits (266), Expect = 5.9e-23
Identity = 85/309 (27.51%), Postives = 144/309 (46.60%), Query Frame = 0

Query: 340 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 399
           +D+++KL LAP+  VG L FR +    GAD+T GE      +  C   L   +     + 
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 400 RHSSEDLFGVQICGAYPDTV---------SRAVELIDKEC-VIDFIDINMGCPIDIVVNK 459
           + +   +F    C      V          RA++  +  C  +  IDINMGCP    +  
Sbjct: 61  KGTDNVVFST--CDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQG 120

Query: 460 GAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTI 519
           G G+ALL KP  +  ++      +D P+T K+R    +       L   I   G  A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180

Query: 520 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAEWNRHKVESPKLATCMIAR 579
           HGR    R    A W+ I     +A  S+ VI NGDV  Y +++R K  +   A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIKT-ATGAASVMVAR 240

Query: 580 GALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLS- 632
           GA+    +F+  K + +W+       ++ K ++   +  W +D   V++T++ + E ++ 
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290

BLAST of CmaCh19G007050 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 6.6e-14
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 343 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 402
           R K  +AP+     LPFR +C+  GA      M    + T   + +  E+   +      
Sbjct: 87  RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146

Query: 403 LFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSV 462
           LF VQ C   PDT+  A + ++  C  D++DIN+GCP  I      G+ L+     +KS+
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206

Query: 463 VEIASGIVDTPITIKVRT-GYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSK--LA 522
           VE  +  ++ P++ K+R     E   +   ++ D    G   + +HGRTR ++  K   A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266

Query: 523 DWEYINRCSSLAPDSLQVIGNGDV 541
           DW  I    +     + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0J64.6e-25462.57tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=370... [more]
Q7XT074.9e-24863.66tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonic... [more]
Q91XI14.1e-14642.21tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Du... [more]
Q96G462.0e-14543.96tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS... [more]
Q3KRC51.0e-14443.61tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HXQ80.0e+0099.86tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GL240.0e+0097.27tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0K4I10.0e+0090.39tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7... [more]
A0A5A7U7Y00.0e+0090.67tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... [more]
A0A5D3CK470.0e+0090.52tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
XP_022969416.10.0e+0099.86tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima][more]
KAG6572077.10.0e+0097.70tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita ar... [more]
XP_023554076.10.0e+0097.85tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo][more]
XP_022952746.10.0e+0097.27tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
XP_004148548.10.0e+0090.39tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 ... [more]
Match NameE-valueIdentityDescription
AT4G38890.13.2e-25562.57FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.15.9e-2327.51Aldolase-type TIM barrel family protein [more]
AT5G67220.16.6e-1428.43FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 341..583
e-value: 7.8E-71
score: 240.1
IPR041367E3 ligase, CCCH-type zinc fingerPFAMPF18044zf-CCCH_4coord: 103..128
e-value: 6.7E-6
score: 25.7
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 348..615
e-value: 5.6E-48
score: 163.6
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 345..579
e-value: 2.2057E-89
score: 277.068
NoneNo IPR availableGENE3D4.10.1000.10coord: 94..173
e-value: 6.0E-6
score: 28.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 260..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..105
NoneNo IPR availablePANTHERPTHR45846TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKEcoord: 39..697
NoneNo IPR availablePANTHERPTHR45846:SF1TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKEcoord: 39..697
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 345..658
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 429..447
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 99..130
score: 10.727612

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G007050.1CmaCh19G007050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0003677 DNA binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity