CmaCh19G003150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh19G003150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolygalacturonase-like
LocationCma_Chr19: 2736009 .. 2737877 (-)
RNA-Seq ExpressionCmaCh19G003150
SyntenyCmaCh19G003150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGTTAGTCTTGGAGCCAAGCCCGATGGAAAGACCGATGCCTCGCCTGTACTTCAAACAGCATGGGCCAAGGCTTGTGACTCGACAGGACCAGCTACTATTCACGTGCCCATAGGCACATTCTACGTTCAAAGTGGGAGCTTTAATGGGCCTTGTAAGAACAATGCTATTACTATTAGCATCGATGGCACTCTTGTGGCTTCCTCGGACATTCAAGTTCTAGCTAAAACTAAAGCTTGGATTGGGTTTAAAGACGTAAATGGGCTTTCCATTCATGGCGGTGTTATTGATGGGAAAGGATCTGGCATATGGAATTGTAAACACTCTGGGAATAATTGCCCCGATGGAGCACCGGTATGCATTATTTAAGTTGGCTAGATATAACAAAATTTAAAAATTTGTATTTTGGGGAAAGTTGAAAAAAAATGCATTGTACTAATTCTTACTCTCCATTGTTTTGAATTTGTAGAATCTTGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATGTTTAACATTGTGGTGCACGGTTGCCAAAATGTGAACATTCAAGGAGTGAAGGTCTCGGATCCTGGCGATAGTCCGAACACTGATGGCATCCATGTGCAAGAGTCATCAGATGTGACCATCCTCAGTTCCAACATCGGCACCGGTGACGACTGCATCTCAATTGGTCCAGGGACCACCAACTTATGGATAGAAAGTGTTACATGTGGACCAGGGCACGGAATCAGGTAGATGTCAACAAATAATTTTTGGAAATTATTTTGTTGGTATGAAAAATATATTTGAAATTATAAAACCAAATATAAAATTAGATTTTGAAGGAATAAAAACACGTTTTAGAAATTTTTTGATCTTGAACCTAGTGATTTTAACTATTTCCCTATTTGTTGAATATGTGTTTGTAGCATTGGAAGCTTGGGGAAGTCGGCAGATGAGCCTGGAGTGAAAAATGTGACAGTTACATCATCGACTTTTACCGAGACTACAAATGGAGTGAGGATTAAGTCTTGGGGAAGACCAAGTAGTGGGTTCGCTACCGATATTCATTTTAAACACATTACTTTGGAGAATGTGAAAAACCCTATCATAATTGATCAAAACTACTGTCCACATGATGAAGGCTGCCCCCATCAGGTATATTTTCTCATTAATCCTCACATGTTAACTTCTTACCAATTATATTTAACTTTAAATTTGATTAATAGAGATTAAATTTTAAATTTCATACGTAATTGTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCTTCAATGAGTTAGAATTTATCTTGTTCAAGACATGTGTTAAATTAGGAGCACTACTTCCGCTACACTTTTTGGTAATGAATTGTGGTGGACAATGTAACTTTCACATATCTTCAAAAGAGTGTTATATTTTCCACTTTGATCATCATAAGCCTTTAGGGTTTTATTTTATTTTTTTTGTTTTCACATAATAGATCTCGTACCATTCAAGATAGTTGTCCTTATTCAATTTTCATGCCATATTTTGATTGTGTTTATTTGCATGAATAGGATTCGGGAGTAAAAATTAGCGAAGTAACTTACGAAGATATCAAGGGAACATCAGCCACTGAAATAGGAGTCAAATTTGATTGTAGCCTAACAAATCCTTGCATTGGGCTCACCTTGGAAGACATTAAAGTGACTTATAATAATCAAATCGCTAAAGCTTCATGCAAAGGTGCCAAAGGAACTGCCTCGGGTGTGGTTGAACCTGCTAGTTGCTTAGAATAG

mRNA sequence

ATTGTTAGTCTTGGAGCCAAGCCCGATGGAAAGACCGATGCCTCGCCTGTACTTCAAACAGCATGGGCCAAGGCTTGTGACTCGACAGGACCAGCTACTATTCACGTGCCCATAGGCACATTCTACGTTCAAAGTGGGAGCTTTAATGGGCCTTGTAAGAACAATGCTATTACTATTAGCATCGATGGCACTCTTGTGGCTTCCTCGGACATTCAAGTTCTAGCTAAAACTAAAGCTTGGATTGGGTTTAAAGACGTAAATGGGCTTTCCATTCATGGCGGTGTTATTGATGGGAAAGGATCTGGCATATGGAATTGTAAACACTCTGGGAATAATTGCCCCGATGGAGCACCGAATCTTGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATGTTTAACATTGTGGTGCACGGTTGCCAAAATGTGAACATTCAAGGAGTGAAGGTCTCGGATCCTGGCGATAGTCCGAACACTGATGGCATCCATGTGCAAGAGTCATCAGATGTGACCATCCTCAGTTCCAACATCGGCACCGGTGACGACTGCATCTCAATTGTTACATCATCGACTTTTACCGAGACTACAAATGGAGTGAGGATTAAGTCTTGGGGAAGACCAAGTAGTGGGTTCGCTACCGATATTCATTTTAAACACATTACTTTGGAGAATGTGAAAAACCCTATCATAATTGATCAAAACTACTGTCCACATGATGAAGGCTGCCCCCATCAGGATTCGGGAGTAAAAATTAGCGAAGTAACTTACGAAGATATCAAGGGAACATCAGCCACTGAAATAGGAGTCAAATTTGATTGTAGCCTAACAAATCCTTGCATTGGGCTCACCTTGGAAGACATTAAAGTGACTTATAATAATCAAATCGCTAAAGCTTCATGCAAAGGTGCCAAAGGAACTGCCTCGGGTGTGGTTGAACCTGCTAGTTGCTTAGAATAG

Coding sequence (CDS)

ATTGTTAGTCTTGGAGCCAAGCCCGATGGAAAGACCGATGCCTCGCCTGTACTTCAAACAGCATGGGCCAAGGCTTGTGACTCGACAGGACCAGCTACTATTCACGTGCCCATAGGCACATTCTACGTTCAAAGTGGGAGCTTTAATGGGCCTTGTAAGAACAATGCTATTACTATTAGCATCGATGGCACTCTTGTGGCTTCCTCGGACATTCAAGTTCTAGCTAAAACTAAAGCTTGGATTGGGTTTAAAGACGTAAATGGGCTTTCCATTCATGGCGGTGTTATTGATGGGAAAGGATCTGGCATATGGAATTGTAAACACTCTGGGAATAATTGCCCCGATGGAGCACCGAATCTTGAAATCACAAATGCACAAAATGTTATCATCAATGGATTAGCTTCAATTAATAGCCAAATGTTTAACATTGTGGTGCACGGTTGCCAAAATGTGAACATTCAAGGAGTGAAGGTCTCGGATCCTGGCGATAGTCCGAACACTGATGGCATCCATGTGCAAGAGTCATCAGATGTGACCATCCTCAGTTCCAACATCGGCACCGGTGACGACTGCATCTCAATTGTTACATCATCGACTTTTACCGAGACTACAAATGGAGTGAGGATTAAGTCTTGGGGAAGACCAAGTAGTGGGTTCGCTACCGATATTCATTTTAAACACATTACTTTGGAGAATGTGAAAAACCCTATCATAATTGATCAAAACTACTGTCCACATGATGAAGGCTGCCCCCATCAGGATTCGGGAGTAAAAATTAGCGAAGTAACTTACGAAGATATCAAGGGAACATCAGCCACTGAAATAGGAGTCAAATTTGATTGTAGCCTAACAAATCCTTGCATTGGGCTCACCTTGGAAGACATTAAAGTGACTTATAATAATCAAATCGCTAAAGCTTCATGCAAAGGTGCCAAAGGAACTGCCTCGGGTGTGGTTGAACCTGCTAGTTGCTTAGAATAG

Protein sequence

IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITISIDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNLEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTILSSNIGTGDDCISIVTSSTFTETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPASCLE
Homology
BLAST of CmaCh19G003150 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 193/364 (53.02%), Postives = 250/364 (68.68%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           + SLGAK DGKTD++    +AWAKAC S  P  I+VP GTF+++   F+GPCKNNAIT  
Sbjct: 30  VASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGTFFLRDVVFSGPCKNNAITFR 89

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKH-SGNNCPDGAPN 120
           I GTLVA SD +V+     WI F  VNG++I GG++DG+G+ +W CK   G +CP GA  
Sbjct: 90  IAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQGTALWACKACHGESCPSGATT 149

Query: 121 LEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVT 180
           L  +++ N++++GLAS+NSQMF+IV++  QNV +QGV+VS  G+SPNTDGIHVQ SS VT
Sbjct: 150 LGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVSRSGNSPNTDGIHVQMSSGVT 209

Query: 181 ILSSNIGTGDDCISI--------------------------------------VTSSTFT 240
           IL+S I TGDDC+SI                                      V + TF+
Sbjct: 210 ILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSLGKEQEEAGVQNVTVKTVTFS 269

Query: 241 ETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISE 300
            T NG+RIKSWGRPS+GFA +I F+H T+ NV+NPI+IDQ+YCP ++GCP Q SGV+IS+
Sbjct: 270 GTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQHYCPDNKGCPGQVSGVQISD 329

Query: 301 VTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEP 326
           VTYEDI GTSATE+ VKFDCS  +PC  + LED+K+TY NQ A++SC  A GT  GVV+P
Sbjct: 330 VTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKNQAAESSCSHADGTTEGVVQP 389

BLAST of CmaCh19G003150 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.8e-83
Identity = 167/366 (45.63%), Postives = 224/366 (61.20%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNG-PCKNNAITI 60
           ++S GAKPDG  D++     AW  AC S  P TI VP G F V +  F+G  CK   I+I
Sbjct: 35  VLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPISI 94

Query: 61  SIDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHS-GNNCPDGAP 120
            I G++VA  D +++A +K WI F+DV  +SI+GG++D +G+ +W CK++ G+NCP GA 
Sbjct: 95  RIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGAK 154

Query: 121 NLEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDV 180
           +L  + + N+ I+GL SINSQ F+IV+    NVNI GVKVS   +SPNTDGIHV+ S  V
Sbjct: 155 SLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHSV 214

Query: 181 TILSSNIGTGDDCISIVTSST--------------------------------------F 240
            I +S IGTGDDCISI   ST                                      F
Sbjct: 215 HITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVDF 274

Query: 241 TETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKIS 300
             T NGVRIK+WG+ S+ FA +I F+HI ++ VKNPIIIDQ+YC H + CP Q+SGVK+S
Sbjct: 275 MGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCLH-KPCPKQESGVKVS 334

Query: 301 EVTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVE 327
            V YEDI GTS TE+ V  DCS   PC G+ ++D+ +   ++ A+ASC  A G+A+ VV 
Sbjct: 335 NVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSANDVVP 394

BLAST of CmaCh19G003150 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.2e-56
Identity = 136/366 (37.16%), Postives = 194/366 (53.01%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIG-TFYVQSGSFNGPCKNNAITI 60
           + + GAK DGKTD +   + AW KAC + G  T  VP G T+ ++S  F GPCK +    
Sbjct: 70  VSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCK-SLRNF 129

Query: 61  SIDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHG---GVIDGKGSGIW--NCK-HSGNNC 120
            I GTL AS+          W+  +DVN LSI G   G+I+G G   W  +CK      C
Sbjct: 130 QILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKSKPC 189

Query: 121 PDGAPNLEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQ 180
                 L + N +N+ +  L   N+Q   I +  C  V +  V+++ PGDSPNTDGIH+ 
Sbjct: 190 TKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIHIT 249

Query: 181 ESSDVTILSSNIGTGDDCISI--------------------------------------V 240
            + ++ + +S+IGTGDDCISI                                      V
Sbjct: 250 NTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGINV 309

Query: 241 TSSTFTETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDS 300
             + F+E+ NGVRIK++ +  SG A +I F++I +ENVKNPIIIDQ+YC  D+ C  Q+S
Sbjct: 310 DGAKFSESDNGVRIKTY-QGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDKDK-CEDQES 369

Query: 301 GVKISEVTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTA 322
            V++  V Y++I GTSAT++ +  +CS   PC G+ LE++K+    +   ASCK A    
Sbjct: 370 AVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKI----KGGTASCKNANVKN 428

BLAST of CmaCh19G003150 vs. ExPASy Swiss-Prot
Match: W8P8Q3 (Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 PE=1 SV=3)

HSP 1 Score: 216.9 bits (551), Expect = 3.5e-55
Identity = 131/371 (35.31%), Postives = 192/371 (51.75%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           I   GAKP+   DA+  L  AW +AC +  PA I VP G F + +    GPCK   +TI 
Sbjct: 28  ITKFGAKPN--ADATSALLAAWKEACAAAAPAKIVVPAGEFLLNAVKLQGPCK-APLTIE 87

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSI----HGGVIDGKGSGIW---NCKHSG--N 120
           I G   A +D+  +     W+  ++V GL+I     GG  DG+G   W    C  SG  N
Sbjct: 88  IAGNFKAPADVAQMKGEDTWVKIENVQGLTITCLPTGGTFDGQGQAAWKQNKCAQSGMCN 147

Query: 121 NCPDGAPNLEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIH 180
           + P       +TNAQ   I+G+ S+NS+++++ V GC+N+ + G+ +  P DS NTDG+H
Sbjct: 148 SLPYNFRFNTLTNAQ---ISGIKSLNSKLYHMGVMGCKNITLTGLTIDAPKDSLNTDGMH 207

Query: 181 VQESSDVTILSSNIGTGDDCISI------------------------------------- 240
           +  S+ V   +S IGTGDDCIS+                                     
Sbjct: 208 IGRSNGVHATNSKIGTGDDCISMGDGAVDVHVEGITCGPGHGISIGSMGKFANEAPNTGI 267

Query: 241 -VTSSTFTETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQ 300
            V + +FT+T NGVRIKSW       A+D+HF+ IT+ NV NP+IIDQ YCP++      
Sbjct: 268 FVKNCSFTDTDNGVRIKSWMNSFEASASDLHFEDITVTNVLNPVIIDQEYCPYNHCKEKT 327

Query: 301 DSGVKISEVTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKG 325
            S VK+S+++++++ G + +   VK  CS   PC G+ L DI +T+    A + CK  K 
Sbjct: 328 PSKVKLSKISFKNVHGAAKSAEVVKLLCSSAVPCDGVELADIDLTFPGGAAVSQCKNVKP 387

BLAST of CmaCh19G003150 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 7.8e-55
Identity = 129/366 (35.25%), Postives = 193/366 (52.73%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           I   G KP+  +D    L +AW +AC ST  A I +P GT+ +      GPCK   I + 
Sbjct: 27  ISKFGGKPN--SDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCK-APIELQ 86

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHG-GVIDGKGSGI---------WNCKHSG 120
           +DGT+ A +D  V+  T+ W  F  ++ L++ G GV DG+G+ +         W+ K+S 
Sbjct: 87  VDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSN 146

Query: 121 NNCPDGAPNLEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGI 180
           +       N       N I+ G+ S +S+ F+++V GC+N+   G  ++ PGDSPNTDGI
Sbjct: 147 SKV---FMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGI 206

Query: 181 HVQESSDVTILSSNIGTGDDCISI------------------------------------ 240
           H+ +S+DV IL++NIGTGDDC+SI                                    
Sbjct: 207 HMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEG 266

Query: 241 --VTSSTFTETTNGVRIKSW-GRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCP 300
             V + T T T NGVRIK+W   P +   +DIHF+ IT+ NVKNP+IIDQ Y P ++   
Sbjct: 267 ITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSK 326

Query: 301 HQDSGVKISEVTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGA 318
              S +K+S+++++++KGTS T  GV   CS   PC G+ L ++ + +N     A C   
Sbjct: 327 KNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNGAPTTAKCTNV 386

BLAST of CmaCh19G003150 vs. ExPASy TrEMBL
Match: A0A6J1IBR5 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472171 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 8.6e-182
Identity = 326/364 (89.56%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. ExPASy TrEMBL
Match: A0A6J1IEE1 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.7e-177
Identity = 319/364 (87.64%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTD SPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDVSPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGS IWNCKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSDIWNCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQ+FNIVV+GCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQIFNIVVYGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCS TNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSPTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. ExPASy TrEMBL
Match: A0A6J1F362 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439543 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 315/364 (86.54%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIW+CKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWDCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVII+GLASINSQ+FNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIIDGLASINSQIFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           +TNGVRIKSW RPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 STNGVRIKSWARPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCS TNPC GLTLEDIKVTYN+QIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSPTNPCTGLTLEDIKVTYNDQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. ExPASy TrEMBL
Match: A0A6J1I926 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472172 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.1e-173
Identity = 312/364 (85.71%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPA+IHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPASIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWI FKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGA NL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIEFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGARNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTF+E
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSANEPGVKNVTVTSSTFSE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHD+GCPHQDSGVKIS+V
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDKGCPHQDSGVKISDV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSAT+IGVKFDCS TNPC GLTLEDIKVTY++QIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIKVTYDDQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. ExPASy TrEMBL
Match: A0A6J1IDK7 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472169 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.5e-173
Identity = 311/364 (85.44%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPA+IHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPASIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWI FKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGA NL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIEFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGARNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTF+E
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSANEPGVKNVTVTSSTFSE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHD+GCPHQDSGVKIS+V
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDKGCPHQDSGVKISDV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSAT+IGVKFDCS TNPC GLTLEDIKVTY++QIAKASCKGAKGTASG+VEPA
Sbjct: 331 TYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIKVTYDDQIAKASCKGAKGTASGMVEPA 390

BLAST of CmaCh19G003150 vs. NCBI nr
Match: XP_022973590.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 646.0 bits (1665), Expect = 1.8e-181
Identity = 326/364 (89.56%), Postives = 326/364 (89.56%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. NCBI nr
Match: XP_022973863.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 631.7 bits (1628), Expect = 3.5e-177
Identity = 319/364 (87.64%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTD SPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDVSPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGS IWNCKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSDIWNCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQ+FNIVV+GCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQIFNIVVYGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCS TNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSPTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. NCBI nr
Match: XP_022932928.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 625.5 bits (1612), Expect = 2.5e-175
Identity = 315/364 (86.54%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIW+CKHSGNNCPDGAPNL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWDCKHSGNNCPDGAPNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVII+GLASINSQ+FNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIIDGLASINSQIFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTFTE
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSADEPGVKNVTVTSSTFTE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           +TNGVRIKSW RPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHDEGCPHQDSGVKISEV
Sbjct: 271 STNGVRIKSWARPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSATEIGVKFDCS TNPC GLTLEDIKVTYN+QIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATEIGVKFDCSPTNPCTGLTLEDIKVTYNDQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. NCBI nr
Match: XP_022973591.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 619.0 bits (1595), Expect = 2.3e-173
Identity = 312/364 (85.71%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPA+IHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPASIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWI FKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGA NL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIEFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGARNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTF+E
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSANEPGVKNVTVTSSTFSE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHD+GCPHQDSGVKIS+V
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDKGCPHQDSGVKISDV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSAT+IGVKFDCS TNPC GLTLEDIKVTY++QIAKASCKGAKGTASGVVEPA
Sbjct: 331 TYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIKVTYDDQIAKASCKGAKGTASGVVEPA 390

BLAST of CmaCh19G003150 vs. NCBI nr
Match: XP_022973588.1 (polygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 617.8 bits (1592), Expect = 5.2e-173
Identity = 311/364 (85.44%), Postives = 321/364 (88.19%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           IVSLGAKPDGKTDASPVLQTAWAKACDSTGPA+IHVPIGTFYVQSGSFNGPCKNNAITI 
Sbjct: 31  IVSLGAKPDGKTDASPVLQTAWAKACDSTGPASIHVPIGTFYVQSGSFNGPCKNNAITIR 90

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           IDGTLVASSDIQVLAKTKAWI FKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGA NL
Sbjct: 91  IDGTLVASSDIQVLAKTKAWIEFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGARNL 150

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
           EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI
Sbjct: 151 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 210

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
           LSSNIGTGDDCISI                                      VTSSTF+E
Sbjct: 211 LSSNIGTGDDCISIGPGTTNLWIESVTCGPGHGISIGSLGKSANEPGVKNVTVTSSTFSE 270

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           TTNGVRIKSWGRPSSGFATDIHF+HITL+NVKNPIIIDQNYCPHD+GCPHQDSGVKIS+V
Sbjct: 271 TTNGVRIKSWGRPSSGFATDIHFQHITLDNVKNPIIIDQNYCPHDKGCPHQDSGVKISDV 330

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPA 327
           TYEDIKGTSAT+IGVKFDCS TNPC GLTLEDIKVTY++QIAKASCKGAKGTASG+VEPA
Sbjct: 331 TYEDIKGTSATKIGVKFDCSPTNPCTGLTLEDIKVTYDDQIAKASCKGAKGTASGMVEPA 390

BLAST of CmaCh19G003150 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.6e-100
Identity = 180/364 (49.45%), Postives = 240/364 (65.93%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNG-PCKNNAITI 60
           I+S GAKPDGKTD++      WAKAC S  P TI VP G F ++S  F+G  CK  ++T 
Sbjct: 25  ILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGRFLLRSIIFDGSKCKRKSVTF 84

Query: 61  SIDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPN 120
            I GTLVA SD +V+ K   WI F+ ++G+S++GGV+D +G+ +W+CK SG NCP GA +
Sbjct: 85  RIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQGASLWSCKKSGKNCPSGATS 144

Query: 121 LEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVT 180
           +   +++NV+I+GL S+NSQMF++ ++GC NV + GVKVS  G+SPNTDGIHVQ SS V+
Sbjct: 145 IGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVSADGNSPNTDGIHVQSSSTVS 204

Query: 181 ILSSNIGTGDDCISI--------------------------------------VTSSTFT 240
           IL+S I TGDDC+SI                                      V ++TFT
Sbjct: 205 ILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGKESVEVGVQNITVKTATFT 264

Query: 241 ETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISE 300
            T NGVRIKSW RPS+GFA +I F+H  + NV+NPI+IDQNYCP +E CP+Q SG+KIS+
Sbjct: 265 GTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQNYCPGNENCPNQVSGIKISD 324

Query: 301 VTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEP 326
           V + DI GTSATE+GVK DCS   PC G+ ++D+K+TY N+ A   C  A G+ +G   P
Sbjct: 325 VMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRNKPATTDCSHAGGSEAGFQRP 384

BLAST of CmaCh19G003150 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 3.8e-97
Identity = 177/364 (48.63%), Postives = 236/364 (64.84%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNG-PCKNNAITI 60
           ++S GAKPDGKTDA+      W  AC S+ P TI VP G F ++S +F+G  CK   +T 
Sbjct: 23  VLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRFLLRSVTFDGSKCKPKPVTF 82

Query: 61  SIDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPN 120
            IDGTLVA +D +V+     WI F+ ++G++++GGV+D +G+ +W+CK SG NCP GA  
Sbjct: 83  RIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARGASLWDCKKSGKNCPSGATT 142

Query: 121 LEITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVT 180
           +   ++ NV+++GL S+NSQMF++V++GC NV +QGVKV   G+SPNTDGIHVQ SS V+
Sbjct: 143 IGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLAAGNSPNTDGIHVQSSSSVS 202

Query: 181 ILSSNIGTGDDCISI--------------------------------------VTSSTFT 240
           I ++ I TGDDC+SI                                      V + TFT
Sbjct: 203 IFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGKDSVESGVQNVTVKTVTFT 262

Query: 241 ETTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISE 300
            T NGVRIKSW RPSSGFA +I F+H  + NV+NPIIIDQNYCP D  CP Q SG+KIS+
Sbjct: 263 GTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQNYCP-DHDCPRQVSGIKISD 322

Query: 301 VTYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEP 326
           V + DI GTSATE+GVK DCS   PC G+ LED+K+TY N+ A ++C  A G  +G  +P
Sbjct: 323 VLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKPAASACTHAGGIEAGFFQP 382

BLAST of CmaCh19G003150 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 321.6 bits (823), Expect = 7.2e-88
Identity = 162/364 (44.51%), Postives = 229/364 (62.91%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           +VS GAKPDG TD++     AW  AC S   AT+ VP GTF ++  +F GPCK+  IT  
Sbjct: 30  VVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTGTFLLKVITFGGPCKSK-ITFQ 89

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           + GT+VA  D +    + +WI F  VN  S+ GG  D +GSG W+C+ SG NCP G  ++
Sbjct: 90  VTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDARGSGFWSCRKSGQNCPPGVRSI 149

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
              +A++VII+G+ S+NSQ+ ++ ++GC NV ++ +++  PGDSPNTDG  VQ S+ VT+
Sbjct: 150 SFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRLVAPGDSPNTDGFTVQFSTGVTL 209

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
             S + TGDDC++I                                      V+SS FT 
Sbjct: 210 TGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGSLAKQLNEDGVENVTVSSSVFTG 269

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           + NGVRIKSW RPS+GF  ++ F+++ + NV+NPIIIDQNYCP ++GCP + SGVKI++V
Sbjct: 270 SQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIIDQNYCPSNQGCPTEHSGVKITQV 329

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQI-AKASCKGAKGTASGVVEP 326
           TY++I+GTSAT+  +K  CS +NPC G+TL+DIK+TYN    A + C  A G   GV++P
Sbjct: 330 TYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLTYNKGTPATSLCFNAVGKNLGVIQP 389

BLAST of CmaCh19G003150 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 7.9e-87
Identity = 162/359 (45.13%), Postives = 223/359 (62.12%), Query Frame = 0

Query: 5   GAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITISIDGT 64
           GA+ DG+ DA+    TAW+ AC S   A ++VP GT+ V++  F GPCK N IT   DGT
Sbjct: 37  GARGDGRADATKSFLTAWSLACGSRARAMVYVPRGTYLVKNLVFWGPCK-NIITFKNDGT 96

Query: 65  LVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNLEITN 124
           LVA ++   +  +  WI F  VN +S++GG ID +G+G W+C+  G++CP GA ++  + 
Sbjct: 97  LVAPANYWDIGNSGYWILFAKVNRISVYGGTIDARGAGYWSCRKKGSHCPQGARSISFSW 156

Query: 125 AQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTILSSN 184
             NV+++GL+S NSQ  ++ VH   NV I+ V++  P  SPNTDGIHVQ SS VTI    
Sbjct: 157 CNNVLLSGLSSFNSQNMHVTVHHSSNVRIENVRIRAPSGSPNTDGIHVQSSSGVTISGGT 216

Query: 185 IGTGDDCISI--------------------------------------VTSSTFTETTNG 244
           I TGDDCI++                                      VTSS FT+T NG
Sbjct: 217 IATGDDCIALSQGSRNIWIERVNCGPGHGISIGSLGDYANEEGVQNVTVTSSVFTKTQNG 276

Query: 245 VRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEVTYED 304
           VRIK+W RPS GF  ++ F+++ + NV+NP+IIDQNYCP+ +GCP Q SGVKIS VT+ +
Sbjct: 277 VRIKTWARPSRGFVNNVVFRNLIMNNVENPVIIDQNYCPNGKGCPRQSSGVKISGVTFAN 336

Query: 305 IKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQIAKASCKGAKGTASGVVEPASCL 326
           IKGTS T I +K DCS +N C GL L+DIK+TY  + + + C+ A G ASGV+ P +C+
Sbjct: 337 IKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTYMRRSSASYCRNAHGRASGVMVPRNCM 394

BLAST of CmaCh19G003150 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.0e-86
Identity = 162/364 (44.51%), Postives = 227/364 (62.36%), Query Frame = 0

Query: 1   IVSLGAKPDGKTDASPVLQTAWAKACDSTGPATIHVPIGTFYVQSGSFNGPCKNNAITIS 60
           +VS GAKPDG TD++     AW  AC S   AT+ VP GTF ++  +F GPCK+  IT  
Sbjct: 30  VVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGTFLLKVITFGGPCKSK-ITFQ 89

Query: 61  IDGTLVASSDIQVLAKTKAWIGFKDVNGLSIHGGVIDGKGSGIWNCKHSGNNCPDGAPNL 120
           + GT++A  D +    +  WI F  VN  S+ GG  D + +G W+C+ SG NCP G  ++
Sbjct: 90  VAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARANGFWSCRKSGQNCPPGVRSI 149

Query: 121 EITNAQNVIINGLASINSQMFNIVVHGCQNVNIQGVKVSDPGDSPNTDGIHVQESSDVTI 180
              +A++VII+G+ S+NSQ+ ++ ++GC NV ++ VK+  PG+SPNTDG HVQ S+ VT 
Sbjct: 150 SFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVAPGNSPNTDGFHVQHSTGVTF 209

Query: 181 LSSNIGTGDDCISI--------------------------------------VTSSTFTE 240
             S + TGDDC++I                                      V+SS FT 
Sbjct: 210 TGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLAKELKEDGVENVTVSSSVFTG 269

Query: 241 TTNGVRIKSWGRPSSGFATDIHFKHITLENVKNPIIIDQNYCPHDEGCPHQDSGVKISEV 300
           + NGVRIKSW RPS+GF   + F+ + ++NV+NPIIIDQNYCP  EGCP++ SGVKIS+V
Sbjct: 270 SQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQNYCPTHEGCPNEYSGVKISQV 329

Query: 301 TYEDIKGTSATEIGVKFDCSLTNPCIGLTLEDIKVTYNNQI-AKASCKGAKGTASGVVEP 326
           TY++I+GTSAT+  +K  CS ++PC G+TL+DIK+TYN    A + C  A G + GV++P
Sbjct: 330 TYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKGTPATSFCFNAVGKSLGVIQP 389

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489792.2e-10553.02Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228188.8e-8345.63Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
O231473.2e-5637.16Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
W8P8Q33.5e-5535.31Probable galacturan 1,4-alpha-galacturonidase SALK6 OS=Kali turgidum OX=151250 P... [more]
Q403127.8e-5535.25Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IBR58.6e-18289.56polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472171 PE=3 SV=1[more]
A0A6J1IEE11.7e-17787.64polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1[more]
A0A6J1F3621.2e-17586.54polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439543 PE=3 SV=1[more]
A0A6J1I9261.1e-17385.71polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472172 PE=3 SV=1[more]
A0A6J1IDK72.5e-17385.44polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472169 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022973590.11.8e-18189.56polygalacturonase-like [Cucurbita maxima][more]
XP_022973863.13.5e-17787.64polygalacturonase-like [Cucurbita maxima][more]
XP_022932928.12.5e-17586.54polygalacturonase-like [Cucurbita moschata][more]
XP_022973591.12.3e-17385.71polygalacturonase-like [Cucurbita maxima][more]
XP_022973588.15.2e-17385.44polygalacturonase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G59850.11.6e-10049.45Pectin lyase-like superfamily protein [more]
AT2G43870.13.8e-9748.63Pectin lyase-like superfamily protein [more]
AT1G05650.17.2e-8844.51Pectin lyase-like superfamily protein [more]
AT2G43880.17.9e-8745.13Pectin lyase-like superfamily protein [more]
AT1G05660.11.0e-8644.51Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 26..197
e-value: 7.7E-34
score: 117.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 197..321
e-value: 1.5E-33
score: 118.2
coord: 1..196
e-value: 5.9E-49
score: 169.0
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 195..324
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..194
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 195..324
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 2..194
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 2..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh19G003150.1CmaCh19G003150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004650 polygalacturonase activity