CmaCh18G012940 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh18G012940
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionethylene-responsive transcription factor CRF4-like
LocationCma_Chr18: 9977179 .. 9978180 (+)
RNA-Seq ExpressionCmaCh18G012940
SyntenyCmaCh18G012940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGATTCTCTGTTTTCTCATGTTAAGTTCACAGAGCACCGTAGCTTCACCAACAAATTTTCCTCCAGGAAACAGCCCCCGTCCCAAAATCGACTCGTACGGATTGCCGTCACGGACCCTGATGCCACGGATTCCTCCAGCGATGAAGAAGGGGAGTTTTTTGAGAGACAGAGAGTGAAGAGATATGTAAATGAGGTCAAAATCCAGACTGCCTCTCGAAATGCCTTTTTACCCAGTGCCCGGAAGAGGCCTGCCGCGGAGGGTTCGGATTCACGCCGTCCGGCGAAGGCGGCTCCGGTCATGAGTGGGAAGAAATTTCGTGGTGTACGCCAGCGTCCCTGGGGTAAATGGGCGGCGGAGATTAGAGATCCGGCCCGCCGTGTTCGGCTCTGGTTGGGGACTTACGATACGGCGGAGGAAGCTGCTATGGTGTACGATAACGCCGCCATTCAGCTTCGAGGGCCGGATGCTTTGACGAACTTTGCAATACCGCCGCCGCCGCTGCCGGAGACAGAAGTTGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATGACGACTCCTGCGAGGAGTCCCACAATTTGTCATCACCGACTTCGGTTCTACGCTTTAGAACTCCGTCGCTGGAGGAATCCGAAAAGCCCCAAAACTCCATCGGGTTTCAGAAACCGCCGCCGCCGCTTCCGCCGCCGCTTCCGCCGATGGCCGATGGCCAATTACGCGAATGTCAAGGCGAAACTTCGTTCTACGAGGTGTATTATACCGATTTCCCCAAATTCGAGGACGTCTTCCAGCTTCCGTGCTCCGAATCTCCGATGTTCTTGGACGAACAGCCACTGTATTTTGAAGAAAGCATATATAACGAAGGTTTCAGTGATTTGTTTACAAATTTGCCTGATGATTTTGGGTCGCCATTGTTGTCTTCGTCGATCAGTCAAGTGGGCGAGGACTATTTCCAAGATATTTTGATGGGATCGGACCCATTGGTAGTTCTGTGA

mRNA sequence

ATGGAGGATTCTCTGTTTTCTCATGTTAAGTTCACAGAGCACCGTAGCTTCACCAACAAATTTTCCTCCAGGAAACAGCCCCCGTCCCAAAATCGACTCGTACGGATTGCCGTCACGGACCCTGATGCCACGGATTCCTCCAGCGATGAAGAAGGGGAGTTTTTTGAGAGACAGAGAGTGAAGAGATATGTAAATGAGGTCAAAATCCAGACTGCCTCTCGAAATGCCTTTTTACCCAGTGCCCGGAAGAGGCCTGCCGCGGAGGGTTCGGATTCACGCCGTCCGGCGAAGGCGGCTCCGGTCATGAGTGGGAAGAAATTTCGTGGTGTACGCCAGCGTCCCTGGGGTAAATGGGCGGCGGAGATTAGAGATCCGGCCCGCCGTGTTCGGCTCTGGTTGGGGACTTACGATACGGCGGAGGAAGCTGCTATGGTGTACGATAACGCCGCCATTCAGCTTCGAGGGCCGGATGCTTTGACGAACTTTGCAATACCGCCGCCGCCGCTGCCGGAGACAGAAGTTGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATGACGACTCCTGCGAGGAGTCCCACAATTTGTCATCACCGACTTCGGTTCTACGCTTTAGAACTCCGTCGCTGGAGGAATCCGAAAAGCCCCAAAACTCCATCGGGTTTCAGAAACCGCCGCCGCCGCTTCCGCCGCCGCTTCCGCCGATGGCCGATGGCCAATTACGCGAATGTCAAGGCGAAACTTCGTTCTACGAGGTGTATTATACCGATTTCCCCAAATTCGAGGACGTCTTCCAGCTTCCGTGCTCCGAATCTCCGATGTTCTTGGACGAACAGCCACTGTATTTTGAAGAAAGCATATATAACGAAGGTTTCAGTGATTTGTTTACAAATTTGCCTGATGATTTTGGGTCGCCATTGTTGTCTTCGTCGATCAGTCAAGTGGGCGAGGACTATTTCCAAGATATTTTGATGGGATCGGACCCATTGGTAGTTCTGTGA

Coding sequence (CDS)

ATGGAGGATTCTCTGTTTTCTCATGTTAAGTTCACAGAGCACCGTAGCTTCACCAACAAATTTTCCTCCAGGAAACAGCCCCCGTCCCAAAATCGACTCGTACGGATTGCCGTCACGGACCCTGATGCCACGGATTCCTCCAGCGATGAAGAAGGGGAGTTTTTTGAGAGACAGAGAGTGAAGAGATATGTAAATGAGGTCAAAATCCAGACTGCCTCTCGAAATGCCTTTTTACCCAGTGCCCGGAAGAGGCCTGCCGCGGAGGGTTCGGATTCACGCCGTCCGGCGAAGGCGGCTCCGGTCATGAGTGGGAAGAAATTTCGTGGTGTACGCCAGCGTCCCTGGGGTAAATGGGCGGCGGAGATTAGAGATCCGGCCCGCCGTGTTCGGCTCTGGTTGGGGACTTACGATACGGCGGAGGAAGCTGCTATGGTGTACGATAACGCCGCCATTCAGCTTCGAGGGCCGGATGCTTTGACGAACTTTGCAATACCGCCGCCGCCGCTGCCGGAGACAGAAGTTGAGGCGGCGAATATCCCCTCTGTTTCCGGTTCCTATGACGACTCCTGCGAGGAGTCCCACAATTTGTCATCACCGACTTCGGTTCTACGCTTTAGAACTCCGTCGCTGGAGGAATCCGAAAAGCCCCAAAACTCCATCGGGTTTCAGAAACCGCCGCCGCCGCTTCCGCCGCCGCTTCCGCCGATGGCCGATGGCCAATTACGCGAATGTCAAGGCGAAACTTCGTTCTACGAGGTGTATTATACCGATTTCCCCAAATTCGAGGACGTCTTCCAGCTTCCGTGCTCCGAATCTCCGATGTTCTTGGACGAACAGCCACTGTATTTTGAAGAAAGCATATATAACGAAGGTTTCAGTGATTTGTTTACAAATTTGCCTGATGATTTTGGGTCGCCATTGTTGTCTTCGTCGATCAGTCAAGTGGGCGAGGACTATTTCCAAGATATTTTGATGGGATCGGACCCATTGGTAGTTCTGTGA

Protein sequence

MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRVKRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIPSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL
Homology
BLAST of CmaCh18G012940 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.0e-46
Identity = 151/357 (42.30%), Postives = 200/357 (56.02%), Query Frame = 0

Query: 9   VKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGE-----FFERQRVKRY 68
           +KFTEH++ T    S      Q R++RI+VTDPDATDSSSD+E E       +R+RVK++
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEV-----KIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMS--GKKFRGVRQRPWG 128
           VNEV      + T S        R++ A +   +  P  +A   +   KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAI---------PPP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF++          PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPETEVEAAN---IPSVSGSYDDSCEESHN-LSSPTSVLRFRTP-SLEESEKPQNSIGF 248
           P P  + +  N     SV+ S   S   S++ L SP SVL  R+P +++E     +S   
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS--- 251

Query: 249 QKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDF--PKF--EDVFQLPCSESPMFLDE 308
                       P+A   ++E    T+  E  ++DF  P F  +DVF    S  P +L  
Sbjct: 252 ------------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGG 311

Query: 309 QPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDYFQDI--LMGSDPLVVL 334
             L+ E+    +  +D+  N   DFGS L S  +    ED+FQDI  L GSDPL+ +
Sbjct: 312 D-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CmaCh18G012940 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 2.6e-45
Identity = 129/321 (40.19%), Postives = 190/321 (59.19%), Query Frame = 0

Query: 9   VKFTEH----RSFTNKFSSRKQPPSQN--RLVRIAVTDPDATDSSSDEEGEFFERQRVKR 68
           VK+TEH    R +T K S  ++   ++  RLVR+++TD DATDSSSDEE   F R+RVKR
Sbjct: 12  VKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVKR 71

Query: 69  YVNEVKIQTASRN----AFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKW 128
            +NE++++ +S +    +  P+  ++     S  ++P+ +    + KK+RGVRQRPWGKW
Sbjct: 72  LINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSG--QNQKKYRGVRQRPWGKW 131

Query: 129 AAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAAN 188
           AAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF + P P P  E E   
Sbjct: 132 AAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQEP 191

Query: 189 IPSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMAD 248
             ++S S  +S ++S +LSSPTSVL ++T     SE+P +S+        L P   P++ 
Sbjct: 192 ESNMSVSISESMDDSQHLSSPTSVLNYQT---YVSEEPIDSLIKPVKQEFLEPEQEPIS- 251

Query: 249 GQLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTN 308
             L E    T+  + +  D    ++ F     +  +F  +QP+   +   N+ F+DL   
Sbjct: 252 WHLGEGNTNTN-DDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLM-- 311

Query: 309 LPDDFGSPLLSSSISQVGEDY 320
           L D       SS I ++G  +
Sbjct: 312 LFDSNAEEYYSSEIKEIGSSF 321

BLAST of CmaCh18G012940 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.3e-44
Identity = 141/361 (39.06%), Postives = 202/361 (55.96%), Query Frame = 0

Query: 6   FSHVKFTEHRS----FTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGE--FFERQR 65
           F  +K+TEH++    +T K S +       + V I  TDPDATDSSSDE+ E   F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKRYVNEVKIQTASRNAFLPSA---RKRPAAEGSDSRRPAKAAP------VMSG--KKFR 125
           VKR+VNE+ ++ +  N     +   RKR ++   +++ PA +         +SG  KKFR
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFR 126

Query: 126 GVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIP--- 185
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+IP   
Sbjct: 127 GVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQE 186

Query: 186 ------PPPLPETEVEAANIPSVSGSYDDSCEES-HNLSSPTSVLRFRTPSLEESEKPQN 245
                 P P+ E +      P+ + S  +S EE   +LSSPTSVL  R+  +++ ++P  
Sbjct: 187 EEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFK 246

Query: 246 SIGFQKPPPPL--PPPLPPMADGQLRECQG----ETSFYEVYYTDFPKFEDVFQLPCSES 305
           S    KP P +   P      +  L E       +T F + Y+ + P    +F  P  + 
Sbjct: 247 S---AKPEPGVSNAPWWHTGFNTGLGESDDSFPLDTPFLDNYFNESPPEMSIFDQPMDQ- 306

Query: 306 PMFLDEQPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDY--FQDILMGSDPL 332
            +F +   + F + ++  G +    N+ D+    L SSSI  +G  +  F D L+ SD L
Sbjct: 307 -IFCENDDI-FNDMLFLGGET---MNIEDE----LTSSSIKDMGSTFSDFDDSLI-SDLL 353

BLAST of CmaCh18G012940 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.8e-38
Identity = 130/321 (40.50%), Postives = 165/321 (51.40%), Query Frame = 0

Query: 32  RLVRIAVTDPDATDSSSDEEGEF------FERQRVKRYVNEVKIQTASRNAFLPSARKRP 91
           R++RI+VTDP ATDSSSDEE E        +R+RVK+YV EV + +   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKR- 70

Query: 92  AAEGSDSRRPAKAAPVM---SGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEA 151
                  ++     PV+   + +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEA
Sbjct: 71  -------KKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEA 130

Query: 152 AMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIPSVSGSYDDSCEESHNLSSPTSV 211
           A+VYDNAAIQLRGP+A  NF  PPPP+ E   EA+    V G  D        L SP SV
Sbjct: 131 AIVYDNAAIQLRGPNAELNF--PPPPVTENVEEAST--EVKGVSDFIIGGGECLRSPVSV 190

Query: 212 LRFRTP-SLEESEKPQNSIGFQKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDFPKF 271
           L   +P S E +   +  +G                           S  E+     P F
Sbjct: 191 L--ESPFSGESTAVKEEFVG--------------------------VSTAEIVVKKEPSF 250

Query: 272 EDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLF-TNLPDD------FGSPLLSSSISQ 331
                     +P+F D+    F  S+      DLF  NL  D      FG    S   S 
Sbjct: 251 NG----SDFSAPLFSDDDVFGFSTSMSESFGGDLFGDNLFADMSFGSGFGFGSGSGFSSW 287

Query: 332 VGEDYFQDI--LMGSDPLVVL 334
             ED+FQDI  L GSDP++ +
Sbjct: 311 HVEDHFQDIGDLFGSDPVLTV 287

BLAST of CmaCh18G012940 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.9e-33
Identity = 102/244 (41.80%), Postives = 132/244 (54.10%), Query Frame = 0

Query: 9   VKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEE--GEFFERQRVKRYVNE 68
           VKFTE+R+ TN  ++        RLVRI VTDP ATDSSSD++         RVKRYV E
Sbjct: 8   VKFTENRTVTNVAATPSN--GSPRLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKE 67

Query: 69  VKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAAEIRDPA 128
           ++      ++   +ARK    E          +  +  KK+RGVRQRPWGK+AAEIRDP+
Sbjct: 68  IRF-CQGESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPS 127

Query: 129 RRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIPSVSGSY 188
            R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF  PP P P  +++  +      + 
Sbjct: 128 SRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTVS------AC 187

Query: 189 DDSCEESHNLSSPTSVLRFRTPSLEE-----------------SEKPQNSIGFQKPPPPL 234
           D   +   +L SPTSVLRF      E                  E+ ++S G   P P L
Sbjct: 188 DYGRDSRQSLHSPTSVLRFNVNEETEHEIEAIELSPERKSTVIKEEEESSAGLVFPDPYL 242

BLAST of CmaCh18G012940 vs. ExPASy TrEMBL
Match: A0A6J1HXE0 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita maxima OX=3661 GN=LOC111467518 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.4e-187
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ
Sbjct: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP
Sbjct: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 333

BLAST of CmaCh18G012940 vs. ExPASy TrEMBL
Match: A0A6J1G1E0 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662 GN=LOC111449782 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.3e-172
Identity = 310/333 (93.09%), Postives = 318/333 (95.50%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LFS VKFTEHRSFTNKFSSRKQPPSQNR+VRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MDDTLFSPVKFTEHRSFTNKFSSRKQPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNE+KIQTA RN  LPSARKRPAAEGSDSRRP KAAPVM+GKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTAPRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSP+SVLRFRTPSLEESEKPQNS  FQKPP   PPPLPPMAD Q
Sbjct: 181 SVSGSYDDSCEESHNLSSPSSVLRFRTPSLEESEKPQNSSVFQKPP---PPPLPPMADHQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYE YYTDFPKFEDVFQLP SESPMFLD+QPLY EESIYN+GFSD FTNLP
Sbjct: 241 LRECQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNDGFSDWFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 330

BLAST of CmaCh18G012940 vs. ExPASy TrEMBL
Match: A0A0A0LB30 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 245/334 (73.35%), Postives = 279/334 (83.53%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MED+LFS VKFTEHR+FTNKFS++K   S++R+VRI+VTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           K+Y+NE+KIQ+  RN  LPS RKRPA + S+ RR  K  P  +GKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPP-PLPETEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA PPP P+PE EVE ANI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKEVETANI 180

Query: 181 PSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADG 240
           PSVSGSY DS EESHNLSSPTSVL FRTPS EESEKP  S   QKPP        P  D 
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP-------APFVDD 240

Query: 241 QLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNL 300
           Q  ECQGETSF E Y+T+FP+F+  F+ P  E+P+FLD+QPL+FE+SI+N+ FS++FTNL
Sbjct: 241 QFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PDDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           P+DFGSPLLSSSI Q G+DYFQDILMGSDPLVVL
Sbjct: 301 PEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 326

BLAST of CmaCh18G012940 vs. ExPASy TrEMBL
Match: A0A6J1CH70 (ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=3673 GN=LOC111011618 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.9e-129
Identity = 246/339 (72.57%), Postives = 276/339 (81.42%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQP-----PSQNRLVRIAVTDPDATDSSSD-EEGEF 60
           MED+LF  VKFTEHR+FT KFS++KQ      P +NR+VRI+VTDPDATDSSSD EEGEF
Sbjct: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60

Query: 61  FERQRVKRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRP 120
           FERQRVK+YVNE+KI++ SRN+ +P  RKRPA EGS+ RRP K  P  +GKKFRGVRQRP
Sbjct: 61  FERQRVKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEV 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFA PPPP PE E 
Sbjct: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPP-PEKEA 180

Query: 181 EAANIPSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLP 240
           E  NIPSVSGSY DS EESHNLSSPTSVL FRT S EESE P     FQKP P      P
Sbjct: 181 E-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSP------P 240

Query: 241 PMADGQLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSD 300
            +AD Q  ECQGETSF   Y TDFPKFED+FQLP  +  +F DEQPL+FEESI+NEGFS 
Sbjct: 241 SLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQ 300

Query: 301 LFTNLPDDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           +FTN+P+D GSP+LSSSISQ G+DYFQDIL+GSDPLVVL
Sbjct: 301 IFTNMPEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 331

BLAST of CmaCh18G012940 vs. ExPASy TrEMBL
Match: A0A5D3BJ86 (Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold63471G00010 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.5e-128
Identity = 243/335 (72.54%), Postives = 276/335 (82.39%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MED+LFS VKFTEHR+FTNKFS++K   S++R+VRI+VTDPDATDSSSDE+ +FFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKKHHQSESRVVRISVTDPDATDSSSDED-DFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           K+Y+NE+KIQ+  RN  LPS RKRPA + S+ RR  K  P  +GKKFRGVRQRPWGKWAA
Sbjct: 61  KKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPP-PLPETEVEAANI 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFA PP  P+PE EVE  NI
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKEVETTNI 180

Query: 181 PSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADG 240
           PSVSGSY DS EESHNLSSPTSVL FRTPS EESEKP  S   QKPP        P  D 
Sbjct: 181 PSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKPP-------APFVDD 240

Query: 241 QLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNL 300
           Q  ECQGETSF E Y+T+FP+F+  F+ P  ESP+FLD+QPL+FE+SI+N+ FS++FTNL
Sbjct: 241 QFHECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PDDFGSPLL-SSSISQVGEDYFQDILMGSDPLVVL 334
           P+DFGSPLL SSSI Q G+DYFQDIL GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

BLAST of CmaCh18G012940 vs. NCBI nr
Match: XP_022968213.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita maxima])

HSP 1 Score: 664.5 bits (1713), Expect = 4.9e-187
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ
Sbjct: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP
Sbjct: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 333

BLAST of CmaCh18G012940 vs. NCBI nr
Match: XP_023542412.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 619.0 bits (1595), Expect = 2.4e-173
Identity = 311/333 (93.39%), Postives = 320/333 (96.10%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MED+LFS VKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNE+KIQTASRN  LPSARKRPAAEGSDSRRP KAAPVM+GKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTASRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           S+SGSYD SCEESHNLSSPTSVLRFRTPSLEESEKPQNS GFQKPP   PPP+ PMAD Q
Sbjct: 181 SISGSYDSSCEESHNLSSPTSVLRFRTPSLEESEKPQNSSGFQKPP---PPPILPMADDQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYE YYTDFPKFEDVFQLPCSESPMFL++QPL+ EESIYNEGFSDLFTNLP
Sbjct: 241 LRECQGETSFYEEYYTDFPKFEDVFQLPCSESPMFLEDQPLFLEESIYNEGFSDLFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGEDYF+DILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDYFEDILMGSDPLVVL 330

BLAST of CmaCh18G012940 vs. NCBI nr
Match: KAG7013289.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 618.2 bits (1593), Expect = 4.1e-173
Identity = 313/333 (93.99%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MED+LFS VKFTEHRSFTNKFSSRK PPSQNR+VRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRSFTNKFSSRKHPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNE+KIQTASRN  LPSARKRPAAEGSDSRRP KAAPVM+GKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTASRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNS  FQKPP   PPPLPPMAD Q
Sbjct: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSSVFQKPP---PPPLPPMADDQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYE YYTDFPKFEDVFQLP SESPMFLD+QPLY EESIYNEGFSDLFTNLP
Sbjct: 241 LRECQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNEGFSDLFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGED+FQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDFFQDILMGSDPLVVL 330

BLAST of CmaCh18G012940 vs. NCBI nr
Match: XP_022945589.1 (ethylene-responsive transcription factor CRF4-like [Cucurbita moschata])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 310/333 (93.09%), Postives = 318/333 (95.50%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           M+D+LFS VKFTEHRSFTNKFSSRKQPPSQNR+VRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MDDTLFSPVKFTEHRSFTNKFSSRKQPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNE+KIQTA RN  LPSARKRPAAEGSDSRRP KAAPVM+GKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTAPRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSP+SVLRFRTPSLEESEKPQNS  FQKPP   PPPLPPMAD Q
Sbjct: 181 SVSGSYDDSCEESHNLSSPSSVLRFRTPSLEESEKPQNSSVFQKPP---PPPLPPMADHQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYE YYTDFPKFEDVFQLP SESPMFLD+QPLY EESIYN+GFSD FTNLP
Sbjct: 241 LRECQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNDGFSDWFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 330

BLAST of CmaCh18G012940 vs. NCBI nr
Match: KAG6574222.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608.6 bits (1568), Expect = 3.2e-170
Identity = 309/333 (92.79%), Postives = 315/333 (94.59%), Query Frame = 0

Query: 1   MEDSLFSHVKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGEFFERQRV 60
           MED+LFS VKFTEHRSFTNKFSSRK PPSQNR+VRIAVTDPDATDSSSDEEGEFFERQRV
Sbjct: 1   MEDTLFSPVKFTEHRSFTNKFSSRKHPPSQNRVVRIAVTDPDATDSSSDEEGEFFERQRV 60

Query: 61  KRYVNEVKIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKWAA 120
           KRYVNE+KIQTASRN  LPSARKRPAAEGSDSRRP KAAPVM+GKKFRGVRQRPWGKWAA
Sbjct: 61  KRYVNEIKIQTASRNTLLPSARKRPAAEGSDSRRPVKAAPVMNGKKFRGVRQRPWGKWAA 120

Query: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180
           EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP
Sbjct: 121 EIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIP 180

Query: 181 SVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMADGQ 240
           SVSGSYDDSCEESHNLSSPTSVLRF TPSLEESEKPQNS  FQKPPP      PPMAD Q
Sbjct: 181 SVSGSYDDSCEESHNLSSPTSVLRFSTPSLEESEKPQNSSVFQKPPP------PPMADDQ 240

Query: 241 LRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTNLP 300
           LRECQGETSFYE YYTDFPKFEDVFQLP SESPMFLD+QPLY EESIYNEGFSDLFTNLP
Sbjct: 241 LRECQGETSFYEEYYTDFPKFEDVFQLPFSESPMFLDDQPLYLEESIYNEGFSDLFTNLP 300

Query: 301 DDFGSPLLSSSISQVGEDYFQDILMGSDPLVVL 334
           DDFGSPLLSSSISQVGED+FQDILMGSDPLVVL
Sbjct: 301 DDFGSPLLSSSISQVGEDFFQDILMGSDPLVVL 327

BLAST of CmaCh18G012940 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 151/357 (42.30%), Postives = 200/357 (56.02%), Query Frame = 0

Query: 9   VKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGE-----FFERQRVKRY 68
           +KFTEH++ T    S      Q R++RI+VTDPDATDSSSD+E E       +R+RVK++
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEV-----KIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMS--GKKFRGVRQRPWG 128
           VNEV      + T S        R++ A +   +  P  +A   +   KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAI---------PPP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF++          PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPETEVEAAN---IPSVSGSYDDSCEESHN-LSSPTSVLRFRTP-SLEESEKPQNSIGF 248
           P P  + +  N     SV+ S   S   S++ L SP SVL  R+P +++E     +S   
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS--- 251

Query: 249 QKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDF--PKF--EDVFQLPCSESPMFLDE 308
                       P+A   ++E    T+  E  ++DF  P F  +DVF    S  P +L  
Sbjct: 252 ------------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGG 311

Query: 309 QPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDYFQDI--LMGSDPLVVL 334
             L+ E+    +  +D+  N   DFGS L S  +    ED+FQDI  L GSDPL+ +
Sbjct: 312 D-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CmaCh18G012940 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 151/357 (42.30%), Postives = 200/357 (56.02%), Query Frame = 0

Query: 9   VKFTEHRSFTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGE-----FFERQRVKRY 68
           +KFTEH++ T    S      Q R++RI+VTDPDATDSSSD+E E       +R+RVK++
Sbjct: 12  IKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKKF 71

Query: 69  VNEV-----KIQTASRNAFLPSARKRPAAEGSDSRRPAKAAPVMS--GKKFRGVRQRPWG 128
           VNEV      + T S        R++ A +   +  P  +A   +   KKFRGVRQRPWG
Sbjct: 72  VNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPWG 131

Query: 129 KWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAI---------PPP 188
           KWAAEIRDP +RVRLWLGTY+TAEEAAMVYDNAAIQLRGPDALTNF++          PP
Sbjct: 132 KWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAPP 191

Query: 189 PLPETEVEAAN---IPSVSGSYDDSCEESHN-LSSPTSVLRFRTP-SLEESEKPQNSIGF 248
           P P  + +  N     SV+ S   S   S++ L SP SVL  R+P +++E     +S   
Sbjct: 192 PSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVL--RSPFAVDEFSGISSS--- 251

Query: 249 QKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDF--PKF--EDVFQLPCSESPMFLDE 308
                       P+A   ++E    T+  E  ++DF  P F  +DVF    S  P +L  
Sbjct: 252 ------------PVAAVVVKEEPSMTTVSET-FSDFSAPLFSDDDVFDFRSSVVPDYLGG 311

Query: 309 QPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDYFQDI--LMGSDPLVVL 334
             L+ E+    +  +D+  N   DFGS L S  +    ED+FQDI  L GSDPL+ +
Sbjct: 312 D-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

BLAST of CmaCh18G012940 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-46
Identity = 129/321 (40.19%), Postives = 190/321 (59.19%), Query Frame = 0

Query: 9   VKFTEH----RSFTNKFSSRKQPPSQN--RLVRIAVTDPDATDSSSDEEGEFFERQRVKR 68
           VK+TEH    R +T K S  ++   ++  RLVR+++TD DATDSSSDEE   F R+RVKR
Sbjct: 12  VKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVKR 71

Query: 69  YVNEVKIQTASRN----AFLPSARKRPAAEGSDSRRPAKAAPVMSGKKFRGVRQRPWGKW 128
            +NE++++ +S +    +  P+  ++     S  ++P+ +    + KK+RGVRQRPWGKW
Sbjct: 72  LINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSG--QNQKKYRGVRQRPWGKW 131

Query: 129 AAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAAN 188
           AAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGPDALTNF + P P P  E E   
Sbjct: 132 AAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQEP 191

Query: 189 IPSVSGSYDDSCEESHNLSSPTSVLRFRTPSLEESEKPQNSIGFQKPPPPLPPPLPPMAD 248
             ++S S  +S ++S +LSSPTSVL ++T     SE+P +S+        L P   P++ 
Sbjct: 192 ESNMSVSISESMDDSQHLSSPTSVLNYQT---YVSEEPIDSLIKPVKQEFLEPEQEPIS- 251

Query: 249 GQLRECQGETSFYEVYYTDFPKFEDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLFTN 308
             L E    T+  + +  D    ++ F     +  +F  +QP+   +   N+ F+DL   
Sbjct: 252 WHLGEGNTNTN-DDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLM-- 311

Query: 309 LPDDFGSPLLSSSISQVGEDY 320
           L D       SS I ++G  +
Sbjct: 312 LFDSNAEEYYSSEIKEIGSSF 321

BLAST of CmaCh18G012940 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 179.5 bits (454), Expect = 4.5e-45
Identity = 141/361 (39.06%), Postives = 202/361 (55.96%), Query Frame = 0

Query: 6   FSHVKFTEHRS----FTNKFSSRKQPPSQNRLVRIAVTDPDATDSSSDEEGE--FFERQR 65
           F  +K+TEH++    +T K S +       + V I  TDPDATDSSSDE+ E   F R+R
Sbjct: 7   FRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRRR 66

Query: 66  VKRYVNEVKIQTASRNAFLPSA---RKRPAAEGSDSRRPAKAAP------VMSG--KKFR 125
           VKR+VNE+ ++ +  N     +   RKR ++   +++ PA +         +SG  KKFR
Sbjct: 67  VKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKKFR 126

Query: 126 GVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFAIP--- 185
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGPDALTNF+IP   
Sbjct: 127 GVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPPQE 186

Query: 186 ------PPPLPETEVEAANIPSVSGSYDDSCEES-HNLSSPTSVLRFRTPSLEESEKPQN 245
                 P P+ E +      P+ + S  +S EE   +LSSPTSVL  R+  +++ ++P  
Sbjct: 187 EEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQPFK 246

Query: 246 SIGFQKPPPPL--PPPLPPMADGQLRECQG----ETSFYEVYYTDFPKFEDVFQLPCSES 305
           S    KP P +   P      +  L E       +T F + Y+ + P    +F  P  + 
Sbjct: 247 S---AKPEPGVSNAPWWHTGFNTGLGESDDSFPLDTPFLDNYFNESPPEMSIFDQPMDQ- 306

Query: 306 PMFLDEQPLYFEESIYNEGFSDLFTNLPDDFGSPLLSSSISQVGEDY--FQDILMGSDPL 332
            +F +   + F + ++  G +    N+ D+    L SSSI  +G  +  F D L+ SD L
Sbjct: 307 -IFCENDDI-FNDMLFLGGET---MNIEDE----LTSSSIKDMGSTFSDFDDSLI-SDLL 353

BLAST of CmaCh18G012940 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 159.1 bits (401), Expect = 6.3e-39
Identity = 130/321 (40.50%), Postives = 165/321 (51.40%), Query Frame = 0

Query: 32  RLVRIAVTDPDATDSSSDEEGEF------FERQRVKRYVNEVKIQTASRNAFLPSARKRP 91
           R++RI+VTDP ATDSSSDEE E        +R+RVK+YV EV + +   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKR- 70

Query: 92  AAEGSDSRRPAKAAPVM---SGKKFRGVRQRPWGKWAAEIRDPARRVRLWLGTYDTAEEA 151
                  ++     PV+   + +KFRGVRQRPWGKWAAEIRDP+RRVR+WLGT+DTAEEA
Sbjct: 71  -------KKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEA 130

Query: 152 AMVYDNAAIQLRGPDALTNFAIPPPPLPETEVEAANIPSVSGSYDDSCEESHNLSSPTSV 211
           A+VYDNAAIQLRGP+A  NF  PPPP+ E   EA+    V G  D        L SP SV
Sbjct: 131 AIVYDNAAIQLRGPNAELNF--PPPPVTENVEEAST--EVKGVSDFIIGGGECLRSPVSV 190

Query: 212 LRFRTP-SLEESEKPQNSIGFQKPPPPLPPPLPPMADGQLRECQGETSFYEVYYTDFPKF 271
           L   +P S E +   +  +G                           S  E+     P F
Sbjct: 191 L--ESPFSGESTAVKEEFVG--------------------------VSTAEIVVKKEPSF 250

Query: 272 EDVFQLPCSESPMFLDEQPLYFEESIYNEGFSDLF-TNLPDD------FGSPLLSSSISQ 331
                     +P+F D+    F  S+      DLF  NL  D      FG    S   S 
Sbjct: 251 NG----SDFSAPLFSDDDVFGFSTSMSESFGGDLFGDNLFADMSFGSGFGFGSGSGFSSW 287

Query: 332 VGEDYFQDI--LMGSDPLVVL 334
             ED+FQDI  L GSDP++ +
Sbjct: 311 HVEDHFQDIGDLFGSDPVLTV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUQ24.0e-4642.30Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUE32.6e-4540.19Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FK126.3e-4439.06Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825038.8e-3840.50Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M3742.9e-3341.80Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1HXE02.4e-187100.00ethylene-responsive transcription factor CRF4-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1G1E06.3e-17293.09ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662... [more]
A0A0A0LB301.1e-13173.35AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=... [more]
A0A6J1CH703.9e-12972.57ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=367... [more]
A0A5D3BJ861.5e-12872.54Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
XP_022968213.14.9e-187100.00ethylene-responsive transcription factor CRF4-like [Cucurbita maxima][more]
XP_023542412.12.4e-17393.39ethylene-responsive transcription factor CRF4-like [Cucurbita pepo subsp. pepo][more]
KAG7013289.14.1e-17393.99Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s... [more]
XP_022945589.11.3e-17193.09ethylene-responsive transcription factor CRF4-like [Cucurbita moschata][more]
KAG6574222.13.2e-17092.79Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
AT4G23750.12.8e-4742.30cytokinin response factor 2 [more]
AT4G23750.22.8e-4742.30cytokinin response factor 2 [more]
AT4G27950.11.8e-4640.19cytokinin response factor 4 [more]
AT5G53290.14.5e-4539.06cytokinin response factor 3 [more]
AT4G11140.16.3e-3940.50cytokinin response factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 129..145
score: 61.5
coord: 107..118
score: 70.91
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 106..169
e-value: 1.4E-38
score: 144.2
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 106..155
e-value: 8.6E-14
score: 51.5
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 106..163
score: 23.762146
IPR001471AP2/ERF domainCDDcd00018AP2coord: 105..164
e-value: 6.89865E-34
score: 116.987
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 105..164
e-value: 2.9E-26
score: 93.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 209..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 183..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..105
NoneNo IPR availablePANTHERPTHR31194:SF137ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF4-LIKEcoord: 8..217
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 8..217
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 106..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh18G012940.1CmaCh18G012940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity