CmaCh18G004380 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh18G004380
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Endoglucanase
Location	Cma_Chr18: 2414600 .. 2416319 (-)
RNA-Seq Expression	CmaCh18G004380
Synteny	CmaCh18G004380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGCAACCAGAGAGATTTGTTCATGCAGAGCACGATGAAGCAGATTATCTCGTTAGCCTGGCACGAGTTAGCTTCAACGTCTCTTCAACATCGACTGTTGATTATGATTCAATTCGTTCTCCATACTACAAGTCCATCGATTTCAAGATCGTTATATCAAATAGAACACGATTCAAATGGTGTTCTTACATTTCAGCTTTTATACTACTTGTAATCATAGCACTTGCACTTCTCCTCAACTTTTTACCTCACAAACATGACAGCCATGAAGCCTCAAACAATCACAAAGTTGCAGTTCATCAAGCTCTAGAGTTTTTCGATGCTCAAAAATGTAAGATCCCCCTTAACTCGAAGCATTCATATAATCTTTCCATGCTCCTAACTGATAAAATCTTTTTCTCAGCTGGTAGGTATCCCGAAAATAGTCCAGTAGACTTTCGAGGAGATTCGGGCTTGGAAGATGGAGTTTCAAGCAATAAACCAGATGGCCTAGTCGGCGGTTTCTATGATTCTGGAAACAATATCAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCTAAGTATGCAGACATGAACGAGCTTGATCATGTAAAGGATATCATCAAATGGGGAACTGATTATTTGCTCAAAGTTTTTGTAGCCCCAAATTCAACTTCTGATCAAACCATAATATATTCTCAGGTAAGCCATATGAGCTCTCAATAAGAACAAATAAGATATTCACAGTGCAGTTATTCCACAATATTGAATCTTCCAATGGCAAGTTTATTACTCAACATTATCTCTAATGCATGGTTGCTAGGTTGGATTTGTCGGTAATTGTAAGATCCCACGTTGATTGGAGAGGGAAATGAAGCATCCCTTATAAAGGTGTAGAAACCTTTCCCTAGCAGATGTGTTTTAAAATTGTGAGGCTAATAAATATATGTAATGGGCTAAAATGGACAATATTTGCTAACGATAGACTTGGATTGTTACAAATGGTATTAGAGCCAGACATTGAACGGTGTGCCAGCGAGGACACTGGCCCCCAAGGGGGGTGGATTGTAAGATCCCACATCAATTGGAAAGGGGAACGAAACATCCCTTATAAGGGTGTGGAACCCTCTCCCTAGCAGACGCATTTTAAAATTGTGAGGTTGACAACGATACGTAACGGGCTAATGCGAACAATATATGCTAACAGTGGACTTGAGCTGTTACAAATGATATCGGAGTCAGACACCAAACAGTGTGCCAGCGAGAACGCTGGCCCCCAAGGGGGGTGGATTGTGAGACCCCACATCAATTGAAGAGGGGAACGAAACATCCCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACATGTTTTAAAATTGGGAGGCTAACGACGTTACGTAAAAGGCTAAAACGGACAATATCTGCTAATGGTGGACTTGAGCTGTTATAATAACGACAGTAAGGCTCAAAATAATATAATAAAATTTATGCTCAAAATAATATAATAAAATAATAAATGGTCTTTGCTCGAAATTTTTGTAGCCCCAAATGCAACTTTTGATCAAACCATAATATATTCTCAGGTAAGTCATATGACCTCTCAATAAGAACAATTAAGATATTCACAGTGCAGTTTATTACTCAACATTATCTCTAATGCATGGTTGCTAGGTAGGATCTGTCACTAACGATAGTAAGGCTTAA

mRNA sequence

ATGATGCAACCAGAGAGATTTGTTCATGCAGAGCACGATGAAGCAGATTATCTCGTTAGCCTGGCACGAGTTAGCTTCAACGTCTCTTCAACATCGACTGTTGATTATGATTCAATTCGTTCTCCATACTACAAGTCCATCGATTTCAAGATCGTTATATCAAATAGAACACGATTCAAATGGTGTTCTTACATTTCAGCTTTTATACTACTTGTAATCATAGCACTTGCACTTCTCCTCAACTTTTTACCTCACAAACATGACAGCCATGAAGCCTCAAACAATCACAAAGTTGCAGTTCATCAAGCTCTAGAGTATCCCGAAAATAGTCCAGTAGACTTTCGAGGAGATTCGGGCTTGGAAGATGGAGTTTCAAGCAATAAACCAGATGGCCTAGTCGGCGGTTTCTATGATTCTGGAAACAATATCAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCTAAGTATGCAGACATGAACGAGCTTGATCATGTAAAGGATATCATCAAATGGGGAACTGATTATTTGCTCAAAGTTTTTGTAGCCCCAAATTCAACTTCTGATCAAACCATAATATATTCTCAGGTAGGATCTGTCACTAACGATAGTAAGGCTTAA

Coding sequence (CDS)

ATGATGCAACCAGAGAGATTTGTTCATGCAGAGCACGATGAAGCAGATTATCTCGTTAGCCTGGCACGAGTTAGCTTCAACGTCTCTTCAACATCGACTGTTGATTATGATTCAATTCGTTCTCCATACTACAAGTCCATCGATTTCAAGATCGTTATATCAAATAGAACACGATTCAAATGGTGTTCTTACATTTCAGCTTTTATACTACTTGTAATCATAGCACTTGCACTTCTCCTCAACTTTTTACCTCACAAACATGACAGCCATGAAGCCTCAAACAATCACAAAGTTGCAGTTCATCAAGCTCTAGAGTATCCCGAAAATAGTCCAGTAGACTTTCGAGGAGATTCGGGCTTGGAAGATGGAGTTTCAAGCAATAAACCAGATGGCCTAGTCGGCGGTTTCTATGATTCTGGAAACAATATCAAGTTCACTTTCCCTACAGCTTATACCATTACTCTTTTAAGCTGGAGTGTGATTGAGTATCATCCTAAGTATGCAGACATGAACGAGCTTGATCATGTAAAGGATATCATCAAATGGGGAACTGATTATTTGCTCAAAGTTTTTGTAGCCCCAAATTCAACTTCTGATCAAACCATAATATATTCTCAGGTAGGATCTGTCACTAACGATAGTAAGGCTTAA

Protein sequence

MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALEYPENSPVDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSKA

Homology

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BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot
Match: O04478 (Endoglucanase 7 OS=Arabidopsis thaliana OX=3702 GN=KOR2 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.5e-30
Identity = 75/192 (39.06%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 37  DSIRSPYYKSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNH 96
           D+ R    K ++   V  +RT F W     A + LV+    +++  LP    +    +N+
Sbjct: 58  DNWRKKKKKYVNLGCVSVSRTVFLWTVGSIAVLFLVVALPIIIVKSLPRHKSAPPPPDNY 117

Query: 97  KVAVHQALEY---------PENSPVDFRGDSGLEDGVSSNKPD---GLVGGFYDSGNNIK 156
            +A+H+AL++         P+ + V +RGDSG +DG+    PD   GLVGG+YD G+N+K
Sbjct: 118 TLALHKALQFFDAQKSGKLPKKNKVSWRGDSGTKDGL----PDVVGGLVGGYYDGGSNVK 177

Query: 157 FTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIY 216
           F FP A+++T+LSWS+IEY  KY  ++E DH++D++KWGTDYLL  F   NS +    IY
Sbjct: 178 FHFPMAFSMTMLSWSLIEYSHKYKAIDEYDHMRDVLKWGTDYLLLTF--NNSATRLDHIY 237

[Close]

BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot
Match: Q38890 (Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 5.4e-28
Identity = 74/181 (40.88%), Postives = 104/181 (57.46%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL 104
           K +D   +I +R  F W         L+   + L++  +P  H      +N+ +A+H+AL
Sbjct: 58  KYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKAL 117

Query: 105 EY---------PENSPVDFRGDSGLEDGVSSNKP--DGLVGGFYDSGNNIKFTFPTAYTI 164
           ++         P+++ V +RG+SGL+DG          LVGG+YD+G+ IKF FP AY +
Sbjct: 118 KFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAM 177

Query: 165 TLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQ-TIIYSQVGSVTN 214
           T+LSWSVIEY  KY    EL HVK++IKWGTDY LK F   NST+D    + SQVGS   
Sbjct: 178 TMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTF---NSTADSIDDLVSQVGSGNT 235

[Close]

BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot
Match: Q7XUK4 (Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 9.2e-28
Identity = 76/179 (42.46%), Postives = 110/179 (61.45%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWC--SYISAFILL---VIIALALLLNFLPHKHDSHEASNNHKVA 104
           K +D   V+  R    W   + ++AFIL+   VIIA +     +P K       + +  A
Sbjct: 60  KYVDLGCVVVKRKLLWWVLWTLLAAFILIGLPVIIAKS-----IPKKKPHAPPPDQYTDA 119

Query: 105 VHQALEY---------PENSPVDFRGDSGLEDGVS-SNKPDGLVGGFYDSGNNIKFTFPT 164
           +H+AL +         P+N+ + +RG+SGL DG   ++   GLVGG+YD+G+NIKF FP 
Sbjct: 120 LHKALLFFNAQKSGRLPKNNGIKWRGNSGLSDGSDLTDVKGGLVGGYYDAGDNIKFHFPL 179

Query: 165 AYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVG 209
           A+++T+LSWSVIEY  KY  + E DHV+++IKWGTDYLL  F +  ST D+  +YSQVG
Sbjct: 180 AFSMTMLSWSVIEYSAKYKAVGEYDHVRELIKWGTDYLLLTFNSSASTIDK--VYSQVG 231

[Close]

BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot
Match: Q84R49 (Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.5e-27
Identity = 68/177 (38.42%), Postives = 105/177 (59.32%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL 104
           K +D   ++ +R  F W       + L I  + +++  +PHK       + +  A+H+AL
Sbjct: 59  KYVDLGCMVLDRKIFMWTVGTILGVGLFIGFVMMIVKLVPHKRPPPPPPDQYTQALHKAL 118

Query: 105 EY---------PENSPVDFRGDSGLEDGVS-SNKPDGLVGGFYDSGNNIKFTFPTAYTIT 164
            +         P+++ V +RG+S ++DG+S S     LVGGFYD+G+ IKF +P A+++T
Sbjct: 119 MFFNAQRSGPLPKHNGVSWRGNSCMKDGLSDSTVRKSLVGGFYDAGDAIKFNYPMAWSMT 178

Query: 165 LLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVT 212
           +LSWSVIEY  KY  + ELDHVK++IKWGTDYLLK F +   T D+ +    VG  +
Sbjct: 179 MLSWSVIEYKAKYEAIGELDHVKELIKWGTDYLLKTFNSSADTIDRIVAQVGVGDTS 235

[Close]

BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot
Match: Q9STW8 (Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-25
Identity = 70/178 (39.33%), Postives = 101/178 (56.74%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL 104
           K +D   ++ +R  F W         L+   + L++  LPH H      +N+ +A+  AL
Sbjct: 58  KYVDLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLPHHHHKEPPPDNYTIALRTAL 117

Query: 105 EY---------PEN-SPVDFRGDSGLEDGVSSNKP--DGLVGGFYDSGNNIKFTFPTAYT 164
           ++         P+N   V +R DS L+DG          LVGG+YD+G++IKF FP +Y 
Sbjct: 118 KFFNAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDAGDSIKFNFPMSYA 177

Query: 165 ITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQT-IIYSQVGS 210
           +T+LSWSVIEY  KY    EL+HVK++IKWGTDY LK F   NS++D   ++  QVGS
Sbjct: 178 MTMLSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTF---NSSADNIYVMVEQVGS 232

[Close]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL
Match: A0A6J1CED2 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111010902 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.4e-87
Identity = 168/223 (75.34%), Postives = 189/223 (84.75%), Query Frame = 0


Query: 1   MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 60
           MMQP R VHAEH EA+ L+S  R+  NVS  +++ YDSI SPY KS DFK+VISNRTRF+
Sbjct: 1   MMQPVRPVHAEH-EANRLLSSTRLDSNVSPRASIGYDSIPSPYSKSFDFKMVISNRTRFR 60

Query: 61  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALE---------YPENSP 120
           WCSYISA +LL+IIA++ LL+FLPHKH+ HEASNNH VA++QAL+         YP+NSP
Sbjct: 61  WCSYISALLLLLIIAVSFLLHFLPHKHNHHEASNNHTVAMNQALKFFDAQKSGRYPKNSP 120

Query: 121 VDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 180
           V FRGDSGLEDGV  NK DGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN
Sbjct: 121 VKFRGDSGLEDGVVDNKLDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 180

Query: 181 ELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDS 215
           ELDHV+DII+WGTDYLLKVFVAPN TSDQ IIYSQVGS +NDS
Sbjct: 181 ELDHVEDIIRWGTDYLLKVFVAPNGTSDQAIIYSQVGSASNDS 222

[Close]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL
Match: A0A5A7UU46 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002590 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.7e-82
Identity = 154/196 (78.57%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 21  LARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFKWCSYISAFILLVIIALALLL 80
           + R +FNV S S+ +Y+SI SPY KS DFKIVISN+ RFK CSYISA +LL+IIAL LLL
Sbjct: 1   MTRHNFNVFSKSSTEYNSIPSPYSKSFDFKIVISNQRRFKCCSYISALLLLLIIALTLLL 60

Query: 81  NFLPHKHDSHEASNNHKVAVHQALEYPENSPVDFRGDSGLEDGVSSNKPDGLVGGFYDSG 140
            FLPHKH+ HEASNN+ VA+  A  YP++SPV FRGDSGL+DGVSSNKPDGL+GGFYDSG
Sbjct: 61  QFLPHKHNLHEASNNYTVALFSAGRYPKSSPVKFRGDSGLKDGVSSNKPDGLIGGFYDSG 120

Query: 141 NNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQ 200
           NN+KFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDII+WGT+YLLKVFVAPN+TSDQ
Sbjct: 121 NNMKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKVFVAPNATSDQ 180

Query: 201 TIIYSQVGSVTNDSKA 217
           TIIYSQVGS +N+SKA
Sbjct: 181 TIIYSQVGSSSNESKA 196

[Close]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL
Match: A0A6P6ANB4 (Endoglucanase OS=Durio zibethinus OX=66656 GN=LOC111311196 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.7e-61
Identity = 128/224 (57.14%), Postives = 161/224 (71.88%), Query Frame = 0


Query: 6   RFVHAEHDEADYLVSLAR-----VSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 65
           RFVHA  + +  L S ++       FNV  +S+    S+ SPY KS D+++VI+++T +K
Sbjct: 14  RFVHASSESSRLLSSASKRNSIEFDFNVRPSSSTGNGSLPSPYSKSYDYELVITDKTYYK 73

Query: 66  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL---------EYPENSP 125
              YIS  +  VI+A+ LL +FLPHK++ H  S N  +AV+QA+          YP NSP
Sbjct: 74  RFLYISLTVAFVILAIGLLPHFLPHKNNQHGPSKNLTLAVNQAITFFDAQKSGNYPSNSP 133

Query: 126 VDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 185
           + FRG SGL+DG  SN P  LVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYH KYAD+ 
Sbjct: 134 IRFRGRSGLQDGNLSNTPADLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHQKYADIG 193

Query: 186 ELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSK 216
           EL H+KD+IKWG+DYLLKVF+APN+TSD TI+YSQVGS  NDS+
Sbjct: 194 ELGHIKDVIKWGSDYLLKVFIAPNATSDPTILYSQVGSAGNDSQ 237

[Close]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL
Match: A0A6J1ACP6 (Endoglucanase OS=Herrania umbratica OX=108875 GN=LOC110416795 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.2e-57
Identity = 121/223 (54.26%), Postives = 157/223 (70.40%), Query Frame = 0


Query: 7   FVHAEHDEADYLVSLAR-----VSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFKW 66
           +VHA  +    L S ++     + F V   S+  YDS+ S Y KS D+++VI+++  +K 
Sbjct: 18  YVHAISEAGRLLPSASKWNSIELDFKVLPQSSTGYDSLPSAYSKSYDYELVITDKAHYKR 77

Query: 67  CSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL---------EYPENSPV 126
             YIS  +  +I+AL L+L+FLP K+  HE+S N  +AV+QA+          YP  SP+
Sbjct: 78  FLYISLTVAFLILALGLVLHFLPRKNHHHESSKNLSLAVNQAITFFDAQKSGNYPSKSPI 137

Query: 127 DFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNE 186
            FRG SGL DG + N    LVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYH KYAD+ E
Sbjct: 138 KFRGSSGLRDGNTRNTRADLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHQKYADIGE 197

Query: 187 LDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSK 216
           L+H+KDII+WG+DYLLKVFVAPN+TS+ TI+YSQVGS  ND++
Sbjct: 198 LEHIKDIIRWGSDYLLKVFVAPNATSEPTILYSQVGSAGNDTR 240

[Close]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL
Match: A0A061FQ48 (Endoglucanase OS=Theobroma cacao OX=3641 GN=TCM_035466 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.2e-56
Identity = 117/223 (52.47%), Postives = 156/223 (69.96%), Query Frame = 0


Query: 7   FVHAEHDEADYLVSLAR-----VSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFKW 66
           +VH   +    L S ++     + F V   S+  YDS+ S Y KS D+++VI+++T +K 
Sbjct: 18  YVHTISEAGRLLPSASKWNSIELDFKVLPQSSTGYDSLPSSYSKSYDYELVITDKTHYKR 77

Query: 67  CSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL---------EYPENSPV 126
             YIS+ +  +I+AL L+L+FLP K+  HE++ N  +AV+QA+          YP  SP+
Sbjct: 78  FLYISSTVAFLILALGLVLHFLPRKNHHHESAKNLSLAVNQAITFFDAQKSGNYPSKSPI 137

Query: 127 DFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNE 186
            FRG SGL DG + N    LVGGFYDSGNNIKFTFP AYTITLLSWSVIEYH KY D+ E
Sbjct: 138 KFRGSSGLRDGNTGNTRADLVGGFYDSGNNIKFTFPAAYTITLLSWSVIEYHQKYEDIGE 197

Query: 187 LDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSK 216
           L+H+KD+I+WG+DYLLKVFVAPN+TS+ TI+YSQVGS  ND++
Sbjct: 198 LEHIKDVIRWGSDYLLKVFVAPNATSEPTILYSQVGSAGNDTQ 240

[Close]

BLAST of CmaCh18G004380 vs. NCBI nr
Match: KAG7012497.1 (Endoglucanase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 207/222 (93.24%), Postives = 212/222 (95.50%), Query Frame = 0


Query: 1   MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 60
           MMQPERFVHAEHDEADYLVS ARVSFNVSSTSTV YDSIRSPYYKSIDFKIVISNRTRFK
Sbjct: 1   MMQPERFVHAEHDEADYLVSTARVSFNVSSTSTVVYDSIRSPYYKSIDFKIVISNRTRFK 60

Query: 61  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALE------YPENSPVDF 120
           WCSYISAF+LLVIIALALLLNFLPHKHDSHEASNNH VAVHQALE      YPENSPVDF
Sbjct: 61  WCSYISAFVLLVIIALALLLNFLPHKHDSHEASNNHTVAVHQALEFFDAQKYPENSPVDF 120

Query: 121 RGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELD 180
           RGDSGL+DGVSS+KPDGLVGGFYDSGNNIKFTFPTAYTITLL WSVIEYHPKYADMNELD
Sbjct: 121 RGDSGLDDGVSSSKPDGLVGGFYDSGNNIKFTFPTAYTITLLGWSVIEYHPKYADMNELD 180

Query: 181 HVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSKA 217
           HVKDIIKWGTDYLLKVFVAPNSTSD+TIIYSQVGSV+NDSKA
Sbjct: 181 HVKDIIKWGTDYLLKVFVAPNSTSDRTIIYSQVGSVSNDSKA 222

[Close]

BLAST of CmaCh18G004380 vs. NCBI nr
Match: KAG6573332.1 (Endoglucanase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 411.8 bits (1057), Expect = 3.7e-111
Identity = 207/225 (92.00%), Postives = 212/225 (94.22%), Query Frame = 0


Query: 1   MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 60
           MMQPERFVHAEHDEADYLVS ARVSFNVSSTSTV YDSIRSPYYKSIDFKIVISNRTRFK
Sbjct: 1   MMQPERFVHAEHDEADYLVSTARVSFNVSSTSTVVYDSIRSPYYKSIDFKIVISNRTRFK 60

Query: 61  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALE---------YPENSP 120
           WCSYISAF+LLVIIALALLLNFLPHKHDSHEASNNH VAVHQALE         YPENSP
Sbjct: 61  WCSYISAFVLLVIIALALLLNFLPHKHDSHEASNNHTVAVHQALEFFDAQKSGRYPENSP 120

Query: 121 VDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 180
           VDFRGDSGL+DGVSS+KPDGLVGGFYDSGNNIKFTFPTAYTITLL WSVIEYHPKYADMN
Sbjct: 121 VDFRGDSGLDDGVSSSKPDGLVGGFYDSGNNIKFTFPTAYTITLLGWSVIEYHPKYADMN 180

Query: 181 ELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSKA 217
           ELDHVKDIIKWGTDYLLKVFVAPNSTSD+TIIYSQVGSV+NDSKA
Sbjct: 181 ELDHVKDIIKWGTDYLLKVFVAPNSTSDRTIIYSQVGSVSNDSKA 225

[Close]

BLAST of CmaCh18G004380 vs. NCBI nr
Match: KAE8653204.1 (hypothetical protein Csa_019838 [Cucumis sativus])

HSP 1 Score: 342.4 bits (877), Expect = 2.8e-90
Identity = 168/216 (77.78%), Postives = 190/216 (87.96%), Query Frame = 0


Query: 1   MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 60
           MMQPER VH EH EAD  +S  R++FN  S S+++Y+SI SPY KS DFKIVISN+ RFK
Sbjct: 1   MMQPERSVHTEH-EADRFLSTTRLNFNAFSESSIEYNSIPSPYSKSFDFKIVISNQRRFK 60

Query: 61  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALEYPENSPVDFRGDSGL 120
           WCSYISA +LL+I+AL LLL FLPHKH+ HEASNN+ VA+  A  YP++SPV FRGDSGL
Sbjct: 61  WCSYISALLLLLIMALTLLLQFLPHKHNLHEASNNYTVALFSAGRYPKSSPVKFRGDSGL 120

Query: 121 EDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDII 180
           EDGVSSNKPDGL+GGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDII
Sbjct: 121 EDGVSSNKPDGLIGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDII 180

Query: 181 KWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSKA 217
           +WGT+YLLK+FVAPN+TSDQTIIYSQVGS +NDS A
Sbjct: 181 RWGTEYLLKIFVAPNATSDQTIIYSQVGSSSNDSNA 215

[Close]

BLAST of CmaCh18G004380 vs. NCBI nr
Match: XP_022140170.1 (endoglucanase 25-like [Momordica charantia])

HSP 1 Score: 331.6 bits (849), Expect = 4.9e-87
Identity = 168/223 (75.34%), Postives = 189/223 (84.75%), Query Frame = 0


Query: 1   MMQPERFVHAEHDEADYLVSLARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFK 60
           MMQP R VHAEH EA+ L+S  R+  NVS  +++ YDSI SPY KS DFK+VISNRTRF+
Sbjct: 1   MMQPVRPVHAEH-EANRLLSSTRLDSNVSPRASIGYDSIPSPYSKSFDFKMVISNRTRFR 60

Query: 61  WCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQALE---------YPENSP 120
           WCSYISA +LL+IIA++ LL+FLPHKH+ HEASNNH VA++QAL+         YP+NSP
Sbjct: 61  WCSYISALLLLLIIAVSFLLHFLPHKHNHHEASNNHTVAMNQALKFFDAQKSGRYPKNSP 120

Query: 121 VDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 180
           V FRGDSGLEDGV  NK DGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN
Sbjct: 121 VKFRGDSGLEDGVVDNKLDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPKYADMN 180

Query: 181 ELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDS 215
           ELDHV+DII+WGTDYLLKVFVAPN TSDQ IIYSQVGS +NDS
Sbjct: 181 ELDHVEDIIRWGTDYLLKVFVAPNGTSDQAIIYSQVGSASNDS 222

[Close]

BLAST of CmaCh18G004380 vs. NCBI nr
Match: KAA0057069.1 (endoglucanase 25 [Cucumis melo var. makuwa])

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-81
Identity = 154/196 (78.57%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 21  LARVSFNVSSTSTVDYDSIRSPYYKSIDFKIVISNRTRFKWCSYISAFILLVIIALALLL 80
           + R +FNV S S+ +Y+SI SPY KS DFKIVISN+ RFK CSYISA +LL+IIAL LLL
Sbjct: 1   MTRHNFNVFSKSSTEYNSIPSPYSKSFDFKIVISNQRRFKCCSYISALLLLLIIALTLLL 60

Query: 81  NFLPHKHDSHEASNNHKVAVHQALEYPENSPVDFRGDSGLEDGVSSNKPDGLVGGFYDSG 140
            FLPHKH+ HEASNN+ VA+  A  YP++SPV FRGDSGL+DGVSSNKPDGL+GGFYDSG
Sbjct: 61  QFLPHKHNLHEASNNYTVALFSAGRYPKSSPVKFRGDSGLKDGVSSNKPDGLIGGFYDSG 120

Query: 141 NNIKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQ 200
           NN+KFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDII+WGT+YLLKVFVAPN+TSDQ
Sbjct: 121 NNMKFTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIRWGTEYLLKVFVAPNATSDQ 180

Query: 201 TIIYSQVGSVTNDSKA 217
           TIIYSQVGS +N+SKA
Sbjct: 181 TIIYSQVGSSSNESKA 196

[Close]

BLAST of CmaCh18G004380 vs. TAIR 10
Match: AT1G65610.1 (Six-hairpin glycosidases superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 5.3e-31
Identity = 75/192 (39.06%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 37  DSIRSPYYKSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNH 96
           D+ R    K ++   V  +RT F W     A + LV+    +++  LP    +    +N+
Sbjct: 58  DNWRKKKKKYVNLGCVSVSRTVFLWTVGSIAVLFLVVALPIIIVKSLPRHKSAPPPPDNY 117

Query: 97  KVAVHQALEY---------PENSPVDFRGDSGLEDGVSSNKPD---GLVGGFYDSGNNIK 156
            +A+H+AL++         P+ + V +RGDSG +DG+    PD   GLVGG+YD G+N+K
Sbjct: 118 TLALHKALQFFDAQKSGKLPKKNKVSWRGDSGTKDGL----PDVVGGLVGGYYDGGSNVK 177

Query: 157 FTFPTAYTITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIY 216
           F FP A+++T+LSWS+IEY  KY  ++E DH++D++KWGTDYLL  F   NS +    IY
Sbjct: 178 FHFPMAFSMTMLSWSLIEYSHKYKAIDEYDHMRDVLKWGTDYLLLTF--NNSATRLDHIY 237

[Close]

BLAST of CmaCh18G004380 vs. TAIR 10
Match: AT5G49720.1 (glycosyl hydrolase 9A1 )

HSP 1 Score: 125.9 bits (315), Expect = 3.8e-29
Identity = 74/181 (40.88%), Postives = 104/181 (57.46%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL 104
           K +D   +I +R  F W         L+   + L++  +P  H      +N+ +A+H+AL
Sbjct: 58  KYVDLGCIIVSRKIFVWTVGTLVAAALLAGFITLIVKTVPRHHPKTPPPDNYTIALHKAL 117

Query: 105 EY---------PENSPVDFRGDSGLEDGVSSNKP--DGLVGGFYDSGNNIKFTFPTAYTI 164
           ++         P+++ V +RG+SGL+DG          LVGG+YD+G+ IKF FP AY +
Sbjct: 118 KFFNAQKSGKLPKHNNVSWRGNSGLQDGKGETGSFYKDLVGGYYDAGDAIKFNFPMAYAM 177

Query: 165 TLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQ-TIIYSQVGSVTN 214
           T+LSWSVIEY  KY    EL HVK++IKWGTDY LK F   NST+D    + SQVGS   
Sbjct: 178 TMLSWSVIEYSAKYEAAGELTHVKELIKWGTDYFLKTF---NSTADSIDDLVSQVGSGNT 235

[Close]

BLAST of CmaCh18G004380 vs. TAIR 10
Match: AT4G24260.1 (glycosyl hydrolase 9A3 )

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-26
Identity = 70/178 (39.33%), Postives = 101/178 (56.74%), Query Frame = 0


Query: 45  KSIDFKIVISNRTRFKWCSYISAFILLVIIALALLLNFLPHKHDSHEASNNHKVAVHQAL 104
           K +D   ++ +R  F W         L+   + L++  LPH H      +N+ +A+  AL
Sbjct: 58  KYVDLGCILVSRKIFLWTLGTIVVTALLSGFITLIVKTLPHHHHKEPPPDNYTIALRTAL 117

Query: 105 EY---------PEN-SPVDFRGDSGLEDGVSSNKP--DGLVGGFYDSGNNIKFTFPTAYT 164
           ++         P+N   V +R DS L+DG          LVGG+YD+G++IKF FP +Y 
Sbjct: 118 KFFNAQQSGKLPKNIYNVSWRHDSCLQDGKGDPGQCYKDLVGGYYDAGDSIKFNFPMSYA 177

Query: 165 ITLLSWSVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQT-IIYSQVGS 210
           +T+LSWSVIEY  KY    EL+HVK++IKWGTDY LK F   NS++D   ++  QVGS
Sbjct: 178 MTMLSWSVIEYSAKYQAAGELEHVKELIKWGTDYFLKTF---NSSADNIYVMVEQVGS 232

[Close]

BLAST of CmaCh18G004380 vs. TAIR 10
Match: AT1G19940.1 (glycosyl hydrolase 9B5 )

HSP 1 Score: 105.1 bits (261), Expect = 7.0e-23
Identity = 65/177 (36.72%), Postives = 100/177 (56.50%), Query Frame = 0


Query: 51  IVISNRTRFKWCSYISAFILLVIIALALLLNFLPHK--HDSHEASN--NHKVAVHQALEY 110
           +V   R+R   CS     I+L+ I +A++   + H+  H   + SN  N+  A+  A+++
Sbjct: 1   MVAKPRSRCCCCSVFIGVIILIAIIIAVIFT-IRHRSNHSDDDGSNVKNYANALKIAMQF 60

Query: 111 --------PENSPVDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSW 170
                    EN+ + +RGDSGL+DG  S     L  G YD+G+++KF FP A+T T+LSW
Sbjct: 61  FDIQKSGKLENNEISWRGDSGLKDG--SEASIDLSKGLYDAGDHMKFGFPMAFTATVLSW 120

Query: 171 SVIEYHPKYADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTNDSK 216
           S++EY  + A +N LDH KD +KW TD+L+    +PN      ++Y QVG    D K
Sbjct: 121 SILEYGDQMASLNLLDHAKDSLKWTTDFLINAHPSPN------VLYIQVGDPVTDHK 168

[Close]

BLAST of CmaCh18G004380 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 45/107 (42.06%), Postives = 71/107 (66.36%), Query Frame = 0


Query: 107 PENSPVDFRGDSGLEDGVSSNKPDGLVGGFYDSGNNIKFTFPTAYTITLLSWSVIEYHPK 166
           P N  V +R DS L DG  +N    L+GG+YD+G+N+KF +P ++T TLLSW+ IEY  +
Sbjct: 44  PTNQRVKWRADSALSDGSLANV--NLIGGYYDAGDNVKFVWPMSFTTTLLSWAAIEYQNE 103

Query: 167 YADMNELDHVKDIIKWGTDYLLKVFVAPNSTSDQTIIYSQVGSVTND 214
            + +N+L +++  IKWGTD++L+   +PN      ++Y+QVG   +D
Sbjct: 104 ISSVNQLGYLRSTIKWGTDFILRAHTSPN------MLYTQVGDGNSD 142

The following BLAST results are available for this feature:

BLAST of CmaCh18G004380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04478	7.5e-30	39.06	Endoglucanase 7 OS=Arabidopsis thaliana OX=3702 GN=KOR2 PE=2 SV=1	[more]
Q38890	5.4e-28	40.88	Endoglucanase 25 OS=Arabidopsis thaliana OX=3702 GN=KOR PE=1 SV=1	[more]
Q7XUK4	9.2e-28	42.46	Endoglucanase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU3 PE=2 SV=2	[more]
Q84R49	3.5e-27	38.42	Endoglucanase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU2 PE=2 SV=1	[more]
Q9STW8	3.3e-25	39.33	Endoglucanase 21 OS=Arabidopsis thaliana OX=3702 GN=KOR3 PE=2 SV=1	[more]

BLAST of CmaCh18G004380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CED2	2.4e-87	75.34	Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111010902 PE=3 SV=1	[more]
A0A5A7UU46	6.7e-82	78.57	Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002590 ...	[more]
A0A6P6ANB4	1.7e-61	57.14	Endoglucanase OS=Durio zibethinus OX=66656 GN=LOC111311196 PE=3 SV=1	[more]
A0A6J1ACP6	1.2e-57	54.26	Endoglucanase OS=Herrania umbratica OX=108875 GN=LOC110416795 PE=3 SV=1	[more]
A0A061FQ48	2.2e-56	52.47	Endoglucanase OS=Theobroma cacao OX=3641 GN=TCM_035466 PE=3 SV=1	[more]

BLAST of CmaCh18G004380 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012497.1	1.7e-111	93.24	Endoglucanase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6573332.1	3.7e-111	92.00	Endoglucanase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAE8653204.1	2.8e-90	77.78	hypothetical protein Csa_019838 [Cucumis sativus]	[more]
XP_022140170.1	4.9e-87	75.34	endoglucanase 25-like [Momordica charantia]	[more]
KAA0057069.1	1.4e-81	78.57	endoglucanase 25 [Cucumis melo var. makuwa]	[more]

BLAST of CmaCh18G004380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65610.1	5.3e-31	39.06	Six-hairpin glycosidases superfamily protein	[more]
AT5G49720.1	3.8e-29	40.88	glycosyl hydrolase 9A1	[more]
AT4G24260.1	2.3e-26	39.33	glycosyl hydrolase 9A3	[more]
AT1G19940.1	7.0e-23	36.72	glycosyl hydrolase 9B5	[more]
AT4G23560.1	1.1e-20	42.06	glycosyl hydrolase 9B15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 97..216 e-value: 5.9E-31 score: 110.0
IPR001701	Glycoside hydrolase family 9	PFAM	PF00759	Glyco_hydro_9	coord: 107..215 e-value: 4.0E-26 score: 92.4
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 26..210
None	No IPR available	PANTHER	PTHR22298:SF109	ENDOGLUCANASE	coord: 26..210
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 107..213

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh18G004380.1	CmaCh18G004380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030245	cellulose catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008810	cellulase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds