CmaCh17G000580 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G000580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCma_Chr17: 258339 .. 260722 (-)
RNA-Seq ExpressionCmaCh17G000580
SyntenyCmaCh17G000580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCACGAGCTTAATGTTGCTATCAATCTACCCAACGACATCTCTTTCCATCTCCACCGTTTTGAATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACCAGCCGCGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAATGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACCCCAAATTATACACTCAAAAGGCTCATACTCTCATGGAAAATGAATGAATCTTGCTCCTTCGCCTCCTCCTCGTCGCCCCGACCACCATCGGTGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCATTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCAGAGCAAAAACCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTAGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTGTTAATCTATCGATCGAACGTGGTTAATAAATCTAAAAATTTCGGTCGATGAAATACGTTACGATATATTCAATCTTTTATTTGTATTCCTAACAGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAAACTAGAAGCGATCAAGTCAATGGTGATCGTTTTGCAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAAATACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAATCAATATTGGAGCTGGCATCGGATGAGATAATCAAGAAAGCAAGTACTATTAAGACGACAGACTCGTCCATAACTACACAGTGGTATGATATTGTTCAAACACGAAATTACATTAGTTGAACGTTTCTCACTAAATCTTTTATTTTTTTCTAGTCGATACGGGACATTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTATTTGCATCATGATCGACCTTCTTCCTGACTCGAATCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCACATCATGGGAATAGCGACGGTGACAAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAATAGAATTTATGTTCGATTGGGTTCTCGGGATTTTCGTTAGGTTAAACTATATATCCCTGAACCTCCAAGATCGTATCTACTCGATTTTTGAACTTTAGGTTAACAGGGTCTTTAAAGTTTAAAAATATCAAGCAGGTCCTTTATCTTCACGGTCATATCCATTAGATTTTTGAACTTTTAAAAGTGTCAAGTTAAAGACCGTAAACTTTCAAATTTACGTTAACAGGGTCTCGAAAATTTAAAAATTTCTAGTCCGTCCTTTACCTTTCAAGGTTGTGTCTACTAAATTTTTTAACTTTCAAAAGTGTCTCGCTAAGATCTTAAACTTTCAATTTGACGTGGAATATGGTCTTTAAACAACTTGTATTAATTTTTTTCTATGTAACAGCCAAAACCCACAGTGAGGGTGACGACAATACGTAATGGGCTGAAGCGGAGAATATCTGCTAGTGGTAGGGTTAGGCTGTTATAAATGGTATCAGAGCTAGACATCGGCCAGTGTGTCAACGAGGACGTTGGACTCCAATAAAGTGGATTGCGAGATCTCAAATCAATTGAAGAGGGAAACGAAACATTTCTTATAAACTGTGAGGCTGACAGCAATACGTAGCGAGCTGAAACAGATAATATTGACTAGTGGTGGACTTGAATTAGTACGACGAAATAAACATAGATAAAATATTATTATCTAAATATACACTCTAGGGTTCTTGCCCAATCAAAAGTCTTCCCATAGTTCTTGAAAAGATGAATGTTTTATTTATTTATTATTTATTTTCTTTATCCTTCTTGAGATCTTCATGG

mRNA sequence

ACCCACGAGCTTAATGTTGCTATCAATCTACCCAACGACATCTCTTTCCATCTCCACCGTTTTGAATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACCAGCCGCGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAATGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACCCCAAATTATACACTCAAAAGGCTCATACTCTCATGGAAAATGAATGAATCTTGCTCCTTCGCCTCCTCCTCGTCGCCCCGACCACCATCGGTGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCATTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCAGAGCAAAAACCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTAGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAAACTAGAAGCGATCAAGTCAATGGTGATCGTTTTGCAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAAATACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAATCAATATTGGAGCTGGCATCGGATGAGATAATCAAGAAAGCAAGTACTATTAAGACGACAGACTCGTCCATAACTACACAGTGTCGATACGGGACATTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTATTTGCATCATGATCGACCTTCTTCCTGACTCGAATCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCACATCATGGGAATAGCGACGGTGACAAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAATAGAATTTATGTTCGATTGGGTTCTCGGGATTTTCGTTAGGTTAAACTATATATCCCTGAACCTCCAAGATCGTATCTACTCGATTTTTGAACTTTAGGTTAACAGGGTCTTTAAAGTTTAAAAATATCAAGCAGGTCCTTTATCTTCACGGTCATATCCATTAGATTTTTGAACTTTTAAAAGTGTCAAGTTAAAGACCGTAAACTTTCAAATTTACGTTAACAGGGTCTCGAAAATTTAAAAATTTCTAGTCCGTCCTTTACCTTTCAAGGTTGTGTCTACTAAATTTTTTAACTTTCAAAAGTGTCTCGCTAAGATCTTAAACTTTCAATTTGACGTGGAATATGGTCTTTAAACAACTTGTATTAATTTTTTTCTATGTAACAGCCAAAACCCACAGTGAGGGTGACGACAATACGTAATGGGCTGAAGCGGAGAATATCTGCTAGTGGTAGGGTTAGGCTGTTATAAATGGTATCAGAGCTAGACATCGGCCAGTGTGTCAACGAGGACGTTGGACTCCAATAAAGTGGATTGCGAGATCTCAAATCAATTGAAGAGGGAAACGAAACATTTCTTATAAACTGTGAGGCTGACAGCAATACGTAGCGAGCTGAAACAGATAATATTGACTAGTGGTGGACTTGAATTAGTACGACGAAATAAACATAGATAAAATATTATTATCTAAATATACACTCTAGGGTTCTTGCCCAATCAAAAGTCTTCCCATAGTTCTTGAAAAGATGAATGTTTTATTTATTTATTATTTATTTTCTTTATCCTTCTTGAGATCTTCATGG

Coding sequence (CDS)

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGCCGCAGCCACCAGCCGCGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAATGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACCCCAAATTATACACTCAAAAGGCTCATACTCTCATGGAAAATGAATGAATCTTGCTCCTTCGCCTCCTCCTCGTCGCCCCGACCACCATCGGTGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCATTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCAGAGCAAAAACCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTAGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAAACTAGAAGCGATCAAGTCAATGGTGATCGTTTTGCAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAAATACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTATCGAAACATCATGCCATTGACTTGTTCAAATCAATATTGGAGCTGGCATCGGATGAGATAATCAAGAAAGCAAGTACTATTAAGACGACAGACTCGTCCATAACTACACAGTGTCGATACGGGACATTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTATTGATCGAAGCTGGTGCTATTTGCATCATGATCGACCTTCTTCCTGACTCGAATCGATCGAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCACATCATGGGAATAGCGACGGTGACAAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

Protein sequence

MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Homology
BLAST of CmaCh17G000580 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 9.1e-46
Identity = 135/426 (31.69%), Postives = 210/426 (49.30%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 78
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 79  TLKRLILSW-KMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMAI 138
           TL+RLI SW  +N S       +PRPP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 139 DDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKT--E 198
           +       K     A   E L  +V       S++  N    +EAL +L     S+T  +
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 199 KLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAIDLFKSIL 258
            L         +KS+  ++QRG  E R YA  +L+ I +      S++     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 259 ELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAICI 318
           ++  D I +KA+                    +A+ +L+ I    +  R   +EAG I +
Sbjct: 251 QILDDRISQKAT-------------------KAAMHILVNICPWGRN-RHKAVEAGVISV 310

Query: 319 MIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATK 378
           +I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+ 
Sbjct: 311 IIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASD 370

Query: 379 IGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWR 437
             V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+
Sbjct: 371 RAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWK 402

BLAST of CmaCh17G000580 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.1e-42
Identity = 132/452 (29.20%), Postives = 224/452 (49.56%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 78
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 79  TLKRLILSW-KMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPF-KASSLKKLRLLMA 138
           TL+RLI SW  +N S       +P+PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 139 ID-------DVAAVRAKTIFVQANGFEVLVGLVTQVA------ICESSNFAN-FEA---- 198
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 199 CEEALGVLSQFPFSKT--EKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 258
            +EAL VL     S+T  + L         +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 259 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 318
                +      +LF  ++++  D+I  KA+                    SA+++L+  
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQILVIT 305

Query: 319 MESSKKCRLLLIEAGAICIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHI 378
               +  R   +E G I ++I+LL D    S R   E  + VL +L  CAEGR     H 
Sbjct: 306 CPWGRN-RHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 365

Query: 379 MGIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRS 438
             IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      
Sbjct: 366 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCG 425

Query: 439 SSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 444
           + TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 NKTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

BLAST of CmaCh17G000580 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.1e-34
Identity = 124/438 (28.31%), Postives = 209/438 (47.72%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 78
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 68

Query: 79  YTLKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMA 138
           + L+RLI  W + NE+      S+PR P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 69  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 128

Query: 139 IDDVAA-VRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTE 198
           ++ +A     + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 129 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 188

Query: 199 KLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAIDLFKSIL 258
               L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK I+
Sbjct: 189 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 248

Query: 259 ELASDEI----------------IKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMES 318
               D +                  + S  KT  S +          ++AL +L+E    
Sbjct: 249 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 308

Query: 319 SKKCRLLLIEAGAICIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIAT 378
           S+  R LL++ GA+  +I+L       K   E +L VL  L  CA GR  +  H  GIA 
Sbjct: 309 SRN-RSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 368

Query: 379 VTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 435
           VTK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KE
Sbjct: 369 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKE 428

BLAST of CmaCh17G000580 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-28
Identity = 118/428 (27.57%), Postives = 198/428 (46.26%), Query Frame = 0

Query: 20  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 79
           IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 80  LKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVALLKNIELSP-------FKASSLKKL 139
           L+RLI  W + N S       +P+ P+ +   V ALL               +A++L++L
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHVSVRSRAAALRRL 133

Query: 140 RLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPF 199
           R      D    + + +    N  E+L+ ++        S   + E   E+L +L   P 
Sbjct: 134 RGFARDSD----KNRVLIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLPI 193

Query: 200 SKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQIS--KKGYDYNSLSKHHAID 259
           ++  +   + S    ++ +  +L   SIE R  A  +++ +S   K  D    S  ++  
Sbjct: 194 TEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSES 253

Query: 260 LFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIE 319
           +F+ +L+L  + I  + + +K    ++   C                  S K  R + I 
Sbjct: 254 VFEGVLDLLRNPISSRRA-LKIGIKTLFALC------------------SVKSTRHIAIT 313

Query: 320 AGAICIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNT 379
           AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +  + K  +  S+ 
Sbjct: 314 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 373

Query: 380 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGS 436
           AT+     L  +C     ER  EE  G G V +L+ ++  +  +   K+KA K++KL   
Sbjct: 374 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERAKKKAQKLLKLLRD 406

BLAST of CmaCh17G000580 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.7e-26
Identity = 109/401 (27.18%), Postives = 191/401 (47.63%), Query Frame = 0

Query: 20  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 79
           +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  PN T
Sbjct: 11  VPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVPNLT 70

Query: 80  LKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMAIDD 139
           L RLI  W  +     A S SP   +   D++ A ++   +     S  K LR     D+
Sbjct: 71  LHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARESDE 130

Query: 140 VAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFE--ACEEALGVLSQFPFSKTEKL 199
                A        G +  V ++  + I +S NF++ +     EA+ +LS        K+
Sbjct: 131 NREFLA--------GKDDFVAMLVDL-ISDSRNFSDSQLLLVGEAVKILSMI----RRKI 190

Query: 200 FE--LLSKL------EAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAID 259
           F+   LS L      + + S  ++++RG+               K   D +++ +  A+D
Sbjct: 191 FDRRRLSNLILTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEFIAVD 250

Query: 260 LFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIE 319
               ++    + ++ +   + ++DS         +L  + L LLI I  SSK+ +L LI 
Sbjct: 251 AESKLIIAKGEGLVTEIIKLISSDSD-------SSLIEANLSLLIAI-ASSKRVKLALIR 310

Query: 320 AGAICIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNT 379
              +  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M  S  
Sbjct: 311 EKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTA 370

Query: 380 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 409
           AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 371 ATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

BLAST of CmaCh17G000580 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 1.8e-251
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK 60
           MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK
Sbjct: 1   MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK 60

Query: 61  TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL 120
           TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL
Sbjct: 61  TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL 120

Query: 121 SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE 180
           SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE
Sbjct: 121 SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE 180

Query: 181 EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN 240
           EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN
Sbjct: 181 EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN 240

Query: 241 SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS 300
           SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS
Sbjct: 241 SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS 300

Query: 301 KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK 360
           KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK
Sbjct: 301 KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK 360

Query: 361 KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK 420
           KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK
Sbjct: 361 KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK 420

Query: 421 IMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           IMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 IMKLHGSFWRRFPCFPYEFRDYFKLIN 447

BLAST of CmaCh17G000580 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.6e-240
Identity = 427/451 (94.68%), Postives = 435/451 (96.45%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI T CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CmaCh17G000580 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.1e-208
Identity = 385/452 (85.18%), Postives = 408/452 (90.27%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQ--PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYN 60
           MEDI QN  PPP    P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFTYN
Sbjct: 1   MEDIHQNPFPPPHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFTYN 60

Query: 61  KKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVA-LLK 120
           KKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCS  SS  P   S+EY DVVA LLK
Sbjct: 61  KKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLP-SSSMEYHDVVADLLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIE SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFEVL+G+VTQVAICESS+FANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSKTEK FELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YD+NSL+KH  IDLFKSILELASDEIIKKAS IKTTDSSI T CRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGIL-NGDGRSSSTK 420
           TVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GIL NGDGR SSTK
Sbjct: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGR-SSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           EKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

BLAST of CmaCh17G000580 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 5.3e-206
Identity = 378/453 (83.44%), Postives = 405/453 (89.40%), Query Frame = 0

Query: 1   MEDIRQNRLPP-----PQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP     P P A+ D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  S+S     S+EY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--STSLLPSSSMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFE+LV +VTQVAICE S+FA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS+ EAIKSM I+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS IKT DSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

BLAST of CmaCh17G000580 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.2e-183
Identity = 347/453 (76.60%), Postives = 374/453 (82.56%), Query Frame = 0

Query: 1   MEDIRQNRLPP-----PQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP     P P A+ D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  S+S     S+EY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--STSLLPSSSMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAV AK +FV+ NGFE+LV +VTQVAICE S+F 
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFT 180

Query: 181 NFEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS+ EAIKSM I+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLI 300
           KGYD+NSL+KH  IDLFKSILELASDEIIKKAS IKTTDSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGA+C+MIDLLPDSNRSKCEKIL++LKLLS+CAEG          
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG---------- 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
                                     +YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 --------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 414

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KEKAMK+MKLHGSFWR+FPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRKFPCFPYEYRDYLKLIN 414

BLAST of CmaCh17G000580 vs. NCBI nr
Match: XP_023006772.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 877.9 bits (2267), Expect = 3.8e-251
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK 60
           MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK
Sbjct: 1   MEDIRQNRLPPPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKK 60

Query: 61  TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL 120
           TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL
Sbjct: 61  TCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIEL 120

Query: 121 SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE 180
           SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE
Sbjct: 121 SPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACE 180

Query: 181 EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN 240
           EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN
Sbjct: 181 EALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYN 240

Query: 241 SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS 300
           SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS
Sbjct: 241 SLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESS 300

Query: 301 KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK 360
           KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK
Sbjct: 301 KKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTK 360

Query: 361 KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK 420
           KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK
Sbjct: 361 KMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMK 420

Query: 421 IMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           IMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 IMKLHGSFWRRFPCFPYEFRDYFKLIN 447

BLAST of CmaCh17G000580 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 844.0 bits (2179), Expect = 6.1e-241
Identity = 431/451 (95.57%), Postives = 437/451 (96.90%), Query Frame = 0

Query: 1   MEDIRQNRLPP----PQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP    P PPA EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCS ASSSSP PPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQVAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSKTEKLFELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKAST+K TDSSI TQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CmaCh17G000580 vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 843.6 bits (2178), Expect = 8.0e-241
Identity = 429/451 (95.12%), Postives = 437/451 (96.90%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI TQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CmaCh17G000580 vs. NCBI nr
Match: XP_022958929.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 427/451 (94.68%), Postives = 435/451 (96.45%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI T CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

BLAST of CmaCh17G000580 vs. NCBI nr
Match: KAG7013396.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 833.6 bits (2152), Expect = 8.2e-238
Identity = 427/451 (94.68%), Postives = 435/451 (96.45%), Query Frame = 0

Query: 1   MEDIRQNRLPPPQ----PPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPPQ    PP  EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCS ASSSSPRPPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL++ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300
           YDYNSLSKHHAIDLFKSILELASDEIIKKAST+K TDSSI T  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 448
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 450

BLAST of CmaCh17G000580 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 186.0 bits (471), Expect = 6.4e-47
Identity = 135/426 (31.69%), Postives = 210/426 (49.30%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 78
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 79  TLKRLILSW-KMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMAI 138
           TL+RLI SW  +N S       +PRPP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 139 DDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKT--E 198
           +       K     A   E L  +V       S++  N    +EAL +L     S+T  +
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 199 KLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAIDLFKSIL 258
            L         +KS+  ++QRG  E R YA  +L+ I +      S++     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 259 ELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAICI 318
           ++  D I +KA+                    +A+ +L+ I    +  R   +EAG I +
Sbjct: 251 QILDDRISQKAT-------------------KAAMHILVNICPWGRN-RHKAVEAGVISV 310

Query: 319 MIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATK 378
           +I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+ 
Sbjct: 311 IIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASD 370

Query: 379 IGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWR 437
             V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+
Sbjct: 371 RAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWK 402

BLAST of CmaCh17G000580 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-43
Identity = 132/452 (29.20%), Postives = 224/452 (49.56%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 78
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 79  TLKRLILSW-KMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPF-KASSLKKLRLLMA 138
           TL+RLI SW  +N S       +P+PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 139 ID-------DVAAVRAKTIFVQANGFEVLVGLVTQVA------ICESSNFAN-FEA---- 198
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 199 CEEALGVLSQFPFSKT--EKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 258
            +EAL VL     S+T  + L         +K++  ++QRG  E R YA  +L+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 259 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 318
                +      +LF  ++++  D+I  KA+                    SA+++L+  
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKAT-------------------RSAMQILVIT 305

Query: 319 MESSKKCRLLLIEAGAICIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHI 378
               +  R   +E G I ++I+LL D    S R   E  + VL +L  CAEGR     H 
Sbjct: 306 CPWGRN-RHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 365

Query: 379 MGIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRS 438
             IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      
Sbjct: 366 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCG 425

Query: 439 SSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 444
           + TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 NKTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

BLAST of CmaCh17G000580 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 146.7 bits (369), Expect = 4.3e-35
Identity = 124/438 (28.31%), Postives = 209/438 (47.72%), Query Frame = 0

Query: 19  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 78
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 82

Query: 79  YTLKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMA 138
           + L+RLI  W + NE+      S+PR P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 83  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 142

Query: 139 IDDVAA-VRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTE 198
           ++ +A     + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 143 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 202

Query: 199 KLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAIDLFKSIL 258
               L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK I+
Sbjct: 203 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 262

Query: 259 ELASDEI----------------IKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMES 318
               D +                  + S  KT  S +          ++AL +L+E    
Sbjct: 263 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 322

Query: 319 SKKCRLLLIEAGAICIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIAT 378
           S+  R LL++ GA+  +I+L       K   E +L VL  L  CA GR  +  H  GIA 
Sbjct: 323 SRN-RSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 382

Query: 379 VTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 435
           VTK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KE
Sbjct: 383 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKE 442

BLAST of CmaCh17G000580 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 129.4 bits (324), Expect = 7.1e-30
Identity = 118/428 (27.57%), Postives = 198/428 (46.26%), Query Frame = 0

Query: 20  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 79
           IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 80  LKRLILSWKM-NESCSFASSSSPRPPSVEYDDVVALLKNIELSP-------FKASSLKKL 139
           L+RLI  W + N S       +P+ P+ +   V ALL               +A++L++L
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHVSVRSRAAALRRL 133

Query: 140 RLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPF 199
           R      D    + + +    N  E+L+ ++        S   + E   E+L +L   P 
Sbjct: 134 RGFARDSD----KNRVLIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLPI 193

Query: 200 SKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQIS--KKGYDYNSLSKHHAID 259
           ++  +   + S    ++ +  +L   SIE R  A  +++ +S   K  D    S  ++  
Sbjct: 194 TEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSES 253

Query: 260 LFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIE 319
           +F+ +L+L  + I  + + +K    ++   C                  S K  R + I 
Sbjct: 254 VFEGVLDLLRNPISSRRA-LKIGIKTLFALC------------------SVKSTRHIAIT 313

Query: 320 AGAICIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNT 379
           AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +  + K  +  S+ 
Sbjct: 314 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 373

Query: 380 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGS 436
           AT+     L  +C     ER  EE  G G V +L+ ++  +  +   K+KA K++KL   
Sbjct: 374 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERAKKKAQKLLKLLRD 406

BLAST of CmaCh17G000580 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-27
Identity = 109/401 (27.18%), Postives = 191/401 (47.63%), Query Frame = 0

Query: 20  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 79
           +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  PN T
Sbjct: 11  VPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVPNLT 70

Query: 80  LKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLKNIELSPFKASSLKKLRLLMAIDD 139
           L RLI  W  +     A S SP   +   D++ A ++   +     S  K LR     D+
Sbjct: 71  LHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARESDE 130

Query: 140 VAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANFE--ACEEALGVLSQFPFSKTEKL 199
                A        G +  V ++  + I +S NF++ +     EA+ +LS        K+
Sbjct: 131 NREFLA--------GKDDFVAMLVDL-ISDSRNFSDSQLLLVGEAVKILSMI----RRKI 190

Query: 200 FE--LLSKL------EAIKSMVIVLQRGSIEGRFYAMEILQQISKKGYDYNSLSKHHAID 259
           F+   LS L      + + S  ++++RG+               K   D +++ +  A+D
Sbjct: 191 FDRRRLSNLILTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEFIAVD 250

Query: 260 LFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEIMESSKKCRLLLIE 319
               ++    + ++ +   + ++DS         +L  + L LLI I  SSK+ +L LI 
Sbjct: 251 AESKLIIAKGEGLVTEIIKLISSDSD-------SSLIEANLSLLIAI-ASSKRVKLALIR 310

Query: 320 AGAICIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNT 379
              +  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M  S  
Sbjct: 311 EKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMKVSTA 370

Query: 380 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 409
           AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 371 ATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TG39.1e-4631.69E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC62.1e-4229.20E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9SF156.1e-3428.31E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT791.0e-2827.57U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9LXE32.7e-2627.18U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1KWQ81.8e-251100.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P... [more]
A0A6J1H6I85.6e-24094.68RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075... [more]
A0A0A0KBP61.1e-20885.18RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE... [more]
A0A1S3CC545.3e-20683.44RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ... [more]
A0A5D3DLY22.2e-18376.60RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_023006772.13.8e-251100.00E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima][more]
XP_023549278.16.1e-24195.57E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo][more]
KAG6574816.18.0e-24195.12E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022958929.11.2e-23994.68E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata][more]
KAG7013396.18.2e-23894.68E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT2G35930.16.4e-4731.69plant U-box 23 [more]
AT3G52450.11.5e-4329.20plant U-box 22 [more]
AT3G11840.14.3e-3528.31plant U-box 24 [more]
AT3G19380.17.1e-3027.57plant U-box 25 [more]
AT5G09800.11.9e-2727.18ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 23..87
e-value: 6.5E-23
score: 92.1
IPR003613U box domainPFAMPF04564U-boxcoord: 20..91
e-value: 2.4E-16
score: 59.7
IPR003613U box domainPROSITEPS51698U_BOXcoord: 19..94
score: 29.502176
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 111..427
e-value: 1.4E-12
score: 48.9
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 11..97
e-value: 2.1E-23
score: 84.1
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 20..445
NoneNo IPR availablePANTHERPTHR22849:SF23RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 20..445
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 24..67
e-value: 6.122E-21
score: 83.437
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 16..90
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 175..422

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G000580.1CmaCh17G000580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity