CmaCh17G000020 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G000020
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiongibberellin 20 oxidase 2-like
LocationCma_Chr17: 9087 .. 10692 (+)
RNA-Seq ExpressionCmaCh17G000020
SyntenyCmaCh17G000020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCAACCAACATGCCAACTTTCTCTTCTCTCTCCGATGATTCTTCTCTCTCCAAAATCCCGTCTCAGTTCATCTGGCCGGACCATGAGAGGCCAACCCTCGACTCGCCCGAGCTTCACGTGCCTCCTATCGACTTGAAGAGCTCAATCTCCGACGACCCAGTTGCTCTCTCTGACACATGTCGTCTCCTCAATCAGGTTTCTTAGACTATCACTAAACTTGGTTAAAAATTATTGGGAGGAAGGTTCACGTGATCATATGAGTAAAGAATACAGATCTATTAGTGAGCTGAAAGCAAGGTCTATGCTCAAAGTGGACAATATCATACTATTTAATGTTAATGTACGTTGGTGTTAATAGGTTTGTCGGAAGCATGGTTTCTTTGTCGTGGTTAACCATGGCGTGGATGCGGAGCTGATATCGAAGGCGCACGAATTCATGGACTTGTTCTTTGGGATGAAGCTTGAGGAGAAGCAGCGAGCGGGGCGGAAGGTGGGCGAGCTGTGTGGGTATGCCAACAGTTTCATAGGGAGATTCTCGTGTAAGCTTCCATGGAAGGAAACTCTGTCCTTCAGATACTCTGCTCATGCTCATTCCTCCACTCTTGTTGAAGATTACTTTGCTAGTGCTTTGGGAGAAGAATTGAGAAGTAGCGGGTAACAATTATTAACACAACACAACAACATCATATGATGCATTATTGTTTATTATTGTTCATTCCCCCAGGAAGTTGTACCAACAATACTGCGACGCCATGAGCAAGGTATCACTGACAATAATGGAGCTTTTGGGGATGAGTTTAGGCGTTGGAAGAGAGTATTTCAGAAACTTCTTTGAAGAAAACGAGTCGATTATGAGATTAAACTACTACCCACCATGCCAAACCCCAGGTCAAACTCTTGGAACTGGGCCTCACTGCGACCCAACTTCCTTGACCATCCTTCATCAAGATCATGTCCACGGCCTCCAAGTTTTCGTCGACCAACAATGGCACTCCATTCCCCCGAATCCCCAAGCTTTCGTGGTCAACATTGGCGACACTTTCACGGTAAGTACAACCCATTCCCACAGACTTCAATTTCTAAGTTCGTAAGTTCAATAAAATGTGTATGATCATTTATCAGGCGCTATCCAATGGAATGTACAAGAGTTGCTTGCACAGGGCAGTGGTGAATGAAGAAAGCGTGAGGAAATCTTTGGCCTTTTTTCTGTGTCCAAATGAAGACAAAGTGGTGAGTCCACCCAGCTGTTTGGTTGACGAGAAAAACCCAAGAATTTTCCCGGATTTCAGATGGCCAACTCTGCTTGAATTTTCTCAGAAACATTACAGAGCCGATGATAAAACCCTAGAGGCCTTCTCCAATTGGCTCTTCCAAACAACACTCTCCACCGATTAATTACCACAATAACCCCCACAACCCCCACAATCTCCTGTTCATACTACAAAACAATATATCCTAAATAAAAAGTTTTAAAAAATAATGTTATTTTATTTTAACGAAATTCATACACGTTTATAAGATACGTCATGTGTATCGATTTCGTTAGAAAAAGCTTAAGAAAAGTTTGATTTTTGGAGATTTCACGGATTATGTTGAAGGATGTGG

mRNA sequence

GCCAACCAACATGCCAACTTTCTCTTCTCTCTCCGATGATTCTTCTCTCTCCAAAATCCCGTCTCAGTTCATCTGGCCGGACCATGAGAGGCCAACCCTCGACTCGCCCGAGCTTCACGTGCCTCCTATCGACTTGAAGAGCTCAATCTCCGACGACCCAGTTGCTCTCTCTGACACATGTCGTCTCCTCAATCAGGCGCACGAATTCATGGACTTGTTCTTTGGGATGAAGCTTGAGGAGAAGCAGCGAGCGGGGCGGAAGGTGGGCGAGCTGTGTGGGTATGCCAACAGTTTCATAGGGAGATTCTCGTGTAAGCTTCCATGGAAGGAAACTCTGTCCTTCAGATACTCTGCTCATGCTCATTCCTCCACTCTTGTTGAAGATTACTTTGCTAGTGCTTTGGGAGAAGAATTGAGAAGTAGCGGGAAGTTGTACCAACAATACTGCGACGCCATGAGCAAGGTATCACTGACAATAATGGAGCTTTTGGGGATGAGTTTAGGCGTTGGAAGAGAGTATTTCAGAAACTTCTTTGAAGAAAACGAGTCGATTATGAGATTAAACTACTACCCACCATGCCAAACCCCAGGTCAAACTCTTGGAACTGGGCCTCACTGCGACCCAACTTCCTTGACCATCCTTCATCAAGATCATGTCCACGGCCTCCAAGTTTTCGTCGACCAACAATGGCACTCCATTCCCCCGAATCCCCAAGCTTTCGTGGTCAACATTGGCGACACTTTCACGGCGCTATCCAATGGAATGTACAAGAGTTGCTTGCACAGGGCAGTGGTGAATGAAGAAAGCGTGAGGAAATCTTTGGCCTTTTTTCTGTGTCCAAATGAAGACAAAGTGGTGAGTCCACCCAGCTGTTTGGTTGACGAGAAAAACCCAAGAATTTTCCCGGATTTCAGATGGCCAACTCTGCTTGAATTTTCTCAGAAACATTACAGAGCCGATGATAAAACCCTAGAGGCCTTCTCCAATTGGCTCTTCCAAACAACACTCTCCACCGATTAATTACCACAATAACCCCCACAACCCCCACAATCTCCTGTTCATACTACAAAACAATATATCCTAAATAAAAAGTTTTAAAAAATAATGTTATTTTATTTTAACGAAATTCATACACGTTTATAAGATACGTCATGTGTATCGATTTCGTTAGAAAAAGCTTAAGAAAAGTTTGATTTTTGGAGATTTCACGGATTATGTTGAAGGATGTGG

Coding sequence (CDS)

ATGCCAACTTTCTCTTCTCTCTCCGATGATTCTTCTCTCTCCAAAATCCCGTCTCAGTTCATCTGGCCGGACCATGAGAGGCCAACCCTCGACTCGCCCGAGCTTCACGTGCCTCCTATCGACTTGAAGAGCTCAATCTCCGACGACCCAGTTGCTCTCTCTGACACATGTCGTCTCCTCAATCAGGCGCACGAATTCATGGACTTGTTCTTTGGGATGAAGCTTGAGGAGAAGCAGCGAGCGGGGCGGAAGGTGGGCGAGCTGTGTGGGTATGCCAACAGTTTCATAGGGAGATTCTCGTGTAAGCTTCCATGGAAGGAAACTCTGTCCTTCAGATACTCTGCTCATGCTCATTCCTCCACTCTTGTTGAAGATTACTTTGCTAGTGCTTTGGGAGAAGAATTGAGAAGTAGCGGGAAGTTGTACCAACAATACTGCGACGCCATGAGCAAGGTATCACTGACAATAATGGAGCTTTTGGGGATGAGTTTAGGCGTTGGAAGAGAGTATTTCAGAAACTTCTTTGAAGAAAACGAGTCGATTATGAGATTAAACTACTACCCACCATGCCAAACCCCAGGTCAAACTCTTGGAACTGGGCCTCACTGCGACCCAACTTCCTTGACCATCCTTCATCAAGATCATGTCCACGGCCTCCAAGTTTTCGTCGACCAACAATGGCACTCCATTCCCCCGAATCCCCAAGCTTTCGTGGTCAACATTGGCGACACTTTCACGGCGCTATCCAATGGAATGTACAAGAGTTGCTTGCACAGGGCAGTGGTGAATGAAGAAAGCGTGAGGAAATCTTTGGCCTTTTTTCTGTGTCCAAATGAAGACAAAGTGGTGAGTCCACCCAGCTGTTTGGTTGACGAGAAAAACCCAAGAATTTTCCCGGATTTCAGATGGCCAACTCTGCTTGAATTTTCTCAGAAACATTACAGAGCCGATGATAAAACCCTAGAGGCCTTCTCCAATTGGCTCTTCCAAACAACACTCTCCACCGATTAA

Protein sequence

MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Homology
BLAST of CmaCh17G000020 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 7.6e-122
Identity = 209/332 (62.95%), Postives = 251/332 (75.60%), Query Frame = 0

Query: 14  SKIPSQFIWPDHERPTLDSPELHVPPIDLKS--SISDDPVALSDTC-------------- 73
           S+IP+QFIWPD E+P++D PEL+VP IDL S  S  + P  +++ C              
Sbjct: 40  SQIPNQFIWPDEEKPSIDIPELNVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVS 99

Query: 74  -RLLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFRYSAH 133
             L+  AH  M+ FF M L  KQ+A RK GE CGYA+SF GRFS KLPWKETLSF++S  
Sbjct: 100 ESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSND 159

Query: 134 AHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREYFRNFFE 193
              S  V+DYF+  LG+E    GK+YQ YC+AMS +SL IMELLG+SLGV R+YFR FFE
Sbjct: 160 NSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFE 219

Query: 194 ENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSIPPNPQA 253
           EN+SIMRLN+YPPCQTP  TLGTGPHCDP+SLTILHQDHV+GLQVFVD QW SI PNP+A
Sbjct: 220 ENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKA 279

Query: 254 FVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLVDEKNPR 313
           FVVNIGDTF ALSNG++KSCLHRAVVN ES RKS+AFFLCP +DKVV PPS ++++   R
Sbjct: 280 FVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTR 339

Query: 314 IFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
            +PDF W   LEF+QKHYRAD  TL++FSNW+
Sbjct: 340 KYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

BLAST of CmaCh17G000020 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 1.6e-116
Identity = 208/336 (61.90%), Postives = 244/336 (72.62%), Query Frame = 0

Query: 14  SKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVAL------SDTCR--------- 73
           + IP+QFIWPD E+P+++  EL VP IDL++ +SD    L      S+ C+         
Sbjct: 38  ANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVN 97

Query: 74  ------LLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFR 133
                 L++ AHE+   FF M L EKQR  RK GE  GYA+SF GRFS KLPWKETLSFR
Sbjct: 98  HGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFR 157

Query: 134 YSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREYFR 193
           +      S  V+DYF  ALG   +  GK+YQ+YC+AMS +SL IMELLG+SLGV R+YFR
Sbjct: 158 FCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFR 217

Query: 194 NFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSIPP 253
            FFEEN+SIMRLNYYPPC  P  TLGTGPHCDPTSLTILHQDHV+GLQVFV+ QW SI P
Sbjct: 218 EFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRP 277

Query: 254 NPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLVDE 313
           NP+AFVVNIGDTF ALSN  YKSCLHRAVVN ES RKSLAFFLCP +D+VV+PP  L+D 
Sbjct: 278 NPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDS 337

Query: 314 KNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
              R +PDF W   LEF+QKHYRAD  TL+AFS+WL
Sbjct: 338 ITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of CmaCh17G000020 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-111
Identity = 204/343 (59.48%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 11  SSLSKIPSQFIWPDHERPTLD-SPELHVPPIDLKSSISDDPVALSDTCRLLNQ------- 70
           S  + IPSQFIWP+ E PT D + ELHVP ID+   +S DP A ++  RL+ +       
Sbjct: 12  SGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGF 71

Query: 71  ---------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKE 130
                          AH  +D FF M L EKQRA R+ GE CGYA+SF GRF+ KLPWKE
Sbjct: 72  FQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131

Query: 131 TLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVG 190
           TLSFR  +      LV DY  + LGE+ R  G++Y +YC  MS++SL IME+LG SLGVG
Sbjct: 132 TLSFR--SCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVG 191

Query: 191 REYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQW 250
           R ++R FFE N+SIMRLNYYPPCQ P +TLGTGPHCDPTSLTILHQD+V GLQV  + +W
Sbjct: 192 RAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 251

Query: 251 HSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPS 310
            SI P   AFVVNIGDTF ALSNG YKSCLHRAVVN +  RKSLAFFLCP  DKVV+PP 
Sbjct: 252 RSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPG 311

Query: 311 CLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQ 331
            LVD  NPR +PDF W +LL+F+QKHYRAD KTLE FS+W+ Q
Sbjct: 312 TLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

BLAST of CmaCh17G000020 vs. ExPASy Swiss-Prot
Match: O04705 (Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 3.5e-111
Identity = 204/343 (59.48%), Postives = 239/343 (69.68%), Query Frame = 0

Query: 11  SSLSKIPSQFIWPDHERPTLD-SPELHVPPIDLKSSISDDPVALSDTCRLLNQ------- 70
           S  + IPSQFIWP+ E PT D + ELHVP ID+   +S DP A ++  RL+ +       
Sbjct: 12  SGRADIPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACERHGF 71

Query: 71  ---------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKE 130
                          AH  +D FF M L EKQRA R  GE CGYA+SF GRF+ KLPWKE
Sbjct: 72  FQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFASKLPWKE 131

Query: 131 TLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVG 190
           TLSFR  +      LV DY  + LGE+ R  G++Y +YC  MS++SL IME+LG SLGVG
Sbjct: 132 TLSFR--SCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVG 191

Query: 191 REYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQW 250
           R ++R FFE N+SIMRLNYYPPCQ P +TLGTGPHCDPTSLTILHQD+V GLQV  + +W
Sbjct: 192 RAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 251

Query: 251 HSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPS 310
            SI P   AFVVNIGDTF ALSNG YKSCLHRAVVN    RKSLAFFLCP  DKVV+PP 
Sbjct: 252 RSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPG 311

Query: 311 CLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQ 331
            LVD  NPR +PDF W +LL+F+QKHYRAD KTLE FS+W+ Q
Sbjct: 312 TLVDAANPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

BLAST of CmaCh17G000020 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-110
Identity = 203/343 (59.18%), Postives = 239/343 (69.68%), Query Frame = 0

Query: 11  SSLSKIPSQFIWPDHERPTLD-SPELHVPPIDLKSSISDDPVALSDTCRLLNQ------- 70
           S  S IPSQFIWP+ E PT D + ELHVP I++   +S D  A ++  RL+ +       
Sbjct: 12  SGRSDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGF 71

Query: 71  ---------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKE 130
                          AH  +D FF M L EKQRA R+ GE CGYA+SF GRF+ KLPWKE
Sbjct: 72  FQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKE 131

Query: 131 TLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVG 190
           TLSFR  +      LV DY  + LGE+ R  G++Y +YC  MS++SL IME+LG SLGVG
Sbjct: 132 TLSFR--SCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVG 191

Query: 191 REYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQW 250
           R ++R FFE N+SIMRLNYYPPCQ P +TLGTGPHCDPTSLTILHQD+V GLQV  + +W
Sbjct: 192 RAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 251

Query: 251 HSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPS 310
            SI P   AFVVNIGDTF ALSNG YKSCLHRAVVN    RKSLAFFLCP  DKVV+PP 
Sbjct: 252 RSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPG 311

Query: 311 CLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQ 331
            LVD  NPR +PDF W +LL+F+QKHYRAD KTLE FS+W+ Q
Sbjct: 312 TLVDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQ 352

BLAST of CmaCh17G000020 vs. ExPASy TrEMBL
Match: A0A6J1L4C7 (gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499029 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 5.4e-195
Identity = 336/358 (93.85%), Postives = 336/358 (93.85%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDAELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 337
           EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Sbjct: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 358

BLAST of CmaCh17G000020 vs. ExPASy TrEMBL
Match: A0A6J1H5L5 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460333 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.5e-192
Identity = 332/358 (92.74%), Postives = 332/358 (92.74%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPV LSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVVLSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDVELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYF SALGEELRSSGKLYQQYCDAMSKVSL IME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFVSALGEELRSSGKLYQQYCDAMSKVSLKIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTP QTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPDQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 337
           EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Sbjct: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 358

BLAST of CmaCh17G000020 vs. ExPASy TrEMBL
Match: A0A6J1D9N4 (gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111018908 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.1e-158
Identity = 274/364 (75.27%), Postives = 307/364 (84.34%), Query Frame = 0

Query: 1   MPTFSSLSD-----DSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSS-ISDDPVALS 60
           MPT S+ SD     DSS+S+IPSQF+WP+ E+P  D PE HVPPIDL ++ +SDDP ALS
Sbjct: 1   MPTLSASSDNPLVLDSSVSQIPSQFVWPEAEKPCHDLPEFHVPPIDLNAAFVSDDPTALS 60

Query: 61  DTCRLLNQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYA 120
           +TCRLLNQ                      AH+FM+ FFGMKLEEKQRA RK  E CGYA
Sbjct: 61  ETCRLLNQVCQKHGFFLVVNHGVDAELIAKAHKFMESFFGMKLEEKQRARRKADEHCGYA 120

Query: 121 NSFIGRFSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKV 180
           NSF+GRFS KLPWKETLSFRY A A SSTLVEDYF  ALGE+ R++GKLYQ+YC+AMSK+
Sbjct: 121 NSFVGRFSSKLPWKETLSFRYCADAASSTLVEDYFVEALGEDFRNAGKLYQEYCEAMSKL 180

Query: 181 SLTIMELLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILH 240
           SL IMELLGMSLGVGR+YFR+FFE+N+S+MRLNYYPPCQ P QTLGTGPHCDPTSLTILH
Sbjct: 181 SLRIMELLGMSLGVGRQYFRDFFEQNDSVMRLNYYPPCQKPDQTLGTGPHCDPTSLTILH 240

Query: 241 QDHVHGLQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLA 300
           QDHVHGLQVFVD++W SI PNP AFV+NIGDTFTAL+NG+YKSCLHRAVVNE +VRKSLA
Sbjct: 241 QDHVHGLQVFVDREWRSIAPNPDAFVINIGDTFTALTNGLYKSCLHRAVVNEGAVRKSLA 300

Query: 301 FFLCPNEDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTT 337
           FFLCPNE+KVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADD+TL AFSNWLFQTT
Sbjct: 301 FFLCPNEEKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDRTLNAFSNWLFQTT 360

BLAST of CmaCh17G000020 vs. ExPASy TrEMBL
Match: S0BDY7 (GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox3 PE=2 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 5.1e-153
Identity = 270/363 (74.38%), Postives = 297/363 (81.82%), Query Frame = 0

Query: 1   MPTFSSLS--------DDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVA 60
           MPT   L+         +S  S IP QFIWPDHE+PTL+ P+LHVPPIDL +S+S DPVA
Sbjct: 1   MPTLDDLNFHNNNIIPSNSPNSIIPPQFIWPDHEKPTLNLPDLHVPPIDLLNSVSGDPVA 60

Query: 61  LSDTCRLLNQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCG 120
           LS+TC LL+Q                      AHE+M+LFFGMK+EEKQRA RKVGE CG
Sbjct: 61  LSETCHLLDQVCRKHGFFIVVNHGVDAELIAKAHEYMELFFGMKVEEKQRAERKVGEHCG 120

Query: 121 YANSFIGRFSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMS 180
           YANSF+GRFSCKLPWKETLSF YSA  +   +VEDYF +ALGE+ R+ GKLYQQYC+AMS
Sbjct: 121 YANSFVGRFSCKLPWKETLSFTYSADCNHD-VVEDYFVNALGEDWRNVGKLYQQYCEAMS 180

Query: 181 KVSLTIMELLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTI 240
           KVSLTIMELLG+SLGVGR+YFR+FF+ NESIMRLNYYP CQ+P QTLGTGPHCDPTSLTI
Sbjct: 181 KVSLTIMELLGLSLGVGRQYFRDFFQRNESIMRLNYYPKCQSPEQTLGTGPHCDPTSLTI 240

Query: 241 LHQ-DHVHGLQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRK 300
           LHQ DHVHGLQVFVDQQWH IPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEE+VRK
Sbjct: 241 LHQHDHVHGLQVFVDQQWHYIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEEAVRK 300

Query: 301 SLAFFLCPNEDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLF 333
           SLAFFLCPNE+KVV PP CLVDEKNPRI  DFRWPT L FSQKHYRADDKTL AFSNWL+
Sbjct: 301 SLAFFLCPNEEKVVRPPGCLVDEKNPRILQDFRWPTFLRFSQKHYRADDKTLLAFSNWLY 360

BLAST of CmaCh17G000020 vs. ExPASy TrEMBL
Match: A0A1S3CBV6 (gibberellin 20 oxidase 1-like OS=Cucumis melo OX=3656 GN=LOC103499045 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.1e-150
Identity = 262/349 (75.07%), Postives = 289/349 (82.81%), Query Frame = 0

Query: 7   LSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLLNQ---- 66
           +  +S  S IP QFIWPDHE+PTL+ P+LHVPPIDL +S+S DPVALS+TC LL+Q    
Sbjct: 15  IPSNSPNSIIPPQFIWPDHEKPTLNLPDLHVPPIDLLNSVSGDPVALSETCHLLDQVCRK 74

Query: 67  ------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLP 126
                             AHE+M+LFFGMK+EEKQRA RKVGE CGYANSF+GRFSCKLP
Sbjct: 75  HGFFIVVNHGVDAELIAKAHEYMELFFGMKVEEKQRAQRKVGEPCGYANSFVGRFSCKLP 134

Query: 127 WKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSL 186
           WKETLSF YSA  +   +VEDYF +ALGE+ RS GKLYQ YC+AMSKVSLTIMELLG+SL
Sbjct: 135 WKETLSFTYSADCNHD-VVEDYFVNALGEDWRSVGKLYQLYCEAMSKVSLTIMELLGLSL 194

Query: 187 GVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQ-DHVHGLQVFV 246
           GVGR YFR+FF+ NESIMRLNYYP CQ P QTLGTGPHCDPTSLTILHQ DHVHGLQVFV
Sbjct: 195 GVGRHYFRDFFQRNESIMRLNYYPKCQNPEQTLGTGPHCDPTSLTILHQHDHVHGLQVFV 254

Query: 247 DQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVV 306
           DQQWH IPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEE+VRKSLAFFLCPNE+KVV
Sbjct: 255 DQQWHYIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEEAVRKSLAFFLCPNEEKVV 314

Query: 307 SPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTT 333
            PP CLVDEKNPRI  DF+WPT   ++QKHYRADDKTL AFSNWL+Q T
Sbjct: 315 RPPGCLVDEKNPRILQDFKWPTFHHYTQKHYRADDKTLLAFSNWLYQQT 362

BLAST of CmaCh17G000020 vs. NCBI nr
Match: XP_023006238.1 (gibberellin 20 oxidase 2-like [Cucurbita maxima])

HSP 1 Score: 689.9 bits (1779), Expect = 1.1e-194
Identity = 336/358 (93.85%), Postives = 336/358 (93.85%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDAELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 337
           EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Sbjct: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 358

BLAST of CmaCh17G000020 vs. NCBI nr
Match: KAG7013346.1 (Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 683.3 bits (1762), Expect = 1.0e-192
Identity = 333/358 (93.02%), Postives = 333/358 (93.02%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDAELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYF SALGEELRSSGKLYQQYCDAMSKVSL IME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFVSALGEELRSSGKLYQQYCDAMSKVSLKIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTP QTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPDQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 337
           EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Sbjct: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 358

BLAST of CmaCh17G000020 vs. NCBI nr
Match: XP_022959323.1 (gibberellin 20 oxidase 2-like [Cucurbita moschata])

HSP 1 Score: 681.8 bits (1758), Expect = 3.0e-192
Identity = 332/358 (92.74%), Postives = 332/358 (92.74%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPV LSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVVLSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDVELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYF SALGEELRSSGKLYQQYCDAMSKVSL IME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFVSALGEELRSSGKLYQQYCDAMSKVSLKIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTP QTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPDQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 337
           EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD
Sbjct: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTLSTD 358

BLAST of CmaCh17G000020 vs. NCBI nr
Match: XP_023549010.1 (gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.9 bits (1735), Expect = 1.4e-189
Identity = 326/355 (91.83%), Postives = 331/355 (93.24%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDD SLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSIS+DPVALSDTCRLL
Sbjct: 1   MPTFSSLSDDPSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISNDPVALSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQICRKHGFFVVVNHGVDAELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTP QTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPDQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQ+WHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQKWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWLFQTTL 334
           EDKVVSPPSCLVD+KNPRIFPDFRWPTLL+FSQKHYRADDKTL+AFSNWLFQTTL
Sbjct: 301 EDKVVSPPSCLVDQKNPRIFPDFRWPTLLQFSQKHYRADDKTLQAFSNWLFQTTL 355

BLAST of CmaCh17G000020 vs. NCBI nr
Match: KAG6574769.1 (Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573.2 bits (1476), Expect = 1.5e-159
Identity = 285/334 (85.33%), Postives = 291/334 (87.13%), Query Frame = 0

Query: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60
           MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL
Sbjct: 1   MPTFSSLSDDSSLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCRLL 60

Query: 61  NQ----------------------AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120
           NQ                      AHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR
Sbjct: 61  NQVCRKHGFFVVVNHGVDAELISKAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 120

Query: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIME 180
           FSCKLPWKETLSFRYSAHAHSSTLVEDYF SALGEELRSSGKLYQQYCDAMSKVSL IME
Sbjct: 121 FSCKLPWKETLSFRYSAHAHSSTLVEDYFVSALGEELRSSGKLYQQYCDAMSKVSLKIME 180

Query: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHG 240
           LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTP QTLGTGPHCDPTSLTILHQDHVHG
Sbjct: 181 LLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPDQTLGTGPHCDPTSLTILHQDHVHG 240

Query: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300
           LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN
Sbjct: 241 LQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPN 300

Query: 301 EDKVVSPPSCLVDEKNPRIFPDFRWPTLLEFSQK 313
           EDKV+     ++ E    +FP F     + FS +
Sbjct: 301 EDKVI---QIILPEFFFLLFPSFNEADSVHFSYR 331

BLAST of CmaCh17G000020 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 438.3 bits (1126), Expect = 5.4e-123
Identity = 209/332 (62.95%), Postives = 251/332 (75.60%), Query Frame = 0

Query: 14  SKIPSQFIWPDHERPTLDSPELHVPPIDLKS--SISDDPVALSDTC-------------- 73
           S+IP+QFIWPD E+P++D PEL+VP IDL S  S  + P  +++ C              
Sbjct: 40  SQIPNQFIWPDEEKPSIDIPELNVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVS 99

Query: 74  -RLLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFRYSAH 133
             L+  AH  M+ FF M L  KQ+A RK GE CGYA+SF GRFS KLPWKETLSF++S  
Sbjct: 100 ESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSND 159

Query: 134 AHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREYFRNFFE 193
              S  V+DYF+  LG+E    GK+YQ YC+AMS +SL IMELLG+SLGV R+YFR FFE
Sbjct: 160 NSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFE 219

Query: 194 ENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSIPPNPQA 253
           EN+SIMRLN+YPPCQTP  TLGTGPHCDP+SLTILHQDHV+GLQVFVD QW SI PNP+A
Sbjct: 220 ENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKA 279

Query: 254 FVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLVDEKNPR 313
           FVVNIGDTF ALSNG++KSCLHRAVVN ES RKS+AFFLCP +DKVV PPS ++++   R
Sbjct: 280 FVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTR 339

Query: 314 IFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
            +PDF W   LEF+QKHYRAD  TL++FSNW+
Sbjct: 340 KYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

BLAST of CmaCh17G000020 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.2e-117
Identity = 208/336 (61.90%), Postives = 244/336 (72.62%), Query Frame = 0

Query: 14  SKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVAL------SDTCR--------- 73
           + IP+QFIWPD E+P+++  EL VP IDL++ +SD    L      S+ C+         
Sbjct: 38  ANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVN 97

Query: 74  ------LLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFR 133
                 L++ AHE+   FF M L EKQR  RK GE  GYA+SF GRFS KLPWKETLSFR
Sbjct: 98  HGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFR 157

Query: 134 YSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREYFR 193
           +      S  V+DYF  ALG   +  GK+YQ+YC+AMS +SL IMELLG+SLGV R+YFR
Sbjct: 158 FCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFR 217

Query: 194 NFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSIPP 253
            FFEEN+SIMRLNYYPPC  P  TLGTGPHCDPTSLTILHQDHV+GLQVFV+ QW SI P
Sbjct: 218 EFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRP 277

Query: 254 NPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLVDE 313
           NP+AFVVNIGDTF ALSN  YKSCLHRAVVN ES RKSLAFFLCP +D+VV+PP  L+D 
Sbjct: 278 NPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDS 337

Query: 314 KNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
              R +PDF W   LEF+QKHYRAD  TL+AFS+WL
Sbjct: 338 ITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373

BLAST of CmaCh17G000020 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 383.6 bits (984), Expect = 1.6e-106
Identity = 193/338 (57.10%), Postives = 230/338 (68.05%), Query Frame = 0

Query: 16  IPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDT------------------- 75
           IP +F+WPDHE+P+ + P L VP IDL   +S+DP+ +S+                    
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 76  ---CRLLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETLSFRY 135
               RLL+ AH+ MD FF     EK +A RKVGE  GYA+SF+GRF   LPWKETLSF +
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 136 SAHAHS---STLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGVGREY 195
           S    S   S  V++Y +  +G+  +  G +YQ+Y + MS +SL IMELLGMSLG+ RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 196 FRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWHSI 255
           FR FFE+NESI RLNYYP C+ P   LGTGPHCDPTSLTIL QD V GLQVFVD QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 256 PPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSCLV 315
           PP PQA VVNIGDT  AL+NG+YKSCLHRAVVN E+ RK+LAFFLCP  DKVV PPS L 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSELE 335

Query: 316 DEKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
            E   R +PDF W   LEF+ KHYRAD  TLE F+NWL
Sbjct: 336 GE---RAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWL 370

BLAST of CmaCh17G000020 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 379.4 bits (973), Expect = 3.0e-105
Identity = 197/354 (55.65%), Postives = 237/354 (66.95%), Query Frame = 0

Query: 5   SSLSDDSSLSK----IPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPVALSDTCR-- 64
           SS+ D   L++    IP QF+WPDHE+P+ D   L VP IDL   +S D    S+  R  
Sbjct: 22  SSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLV 81

Query: 65  --------------------LLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGR 124
                               LL++A+  MD FF     EKQ+A RK GE  GYA+SF+GR
Sbjct: 82  SKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGR 141

Query: 125 FSCKLPWKETLSFRYSAH--AHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTI 184
           FS KLPWKETLSF++S     HS T V+D+ +  +G+     GK+YQ+Y +AM+ +SL I
Sbjct: 142 FSSKLPWKETLSFKFSPEEKIHSQT-VKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKI 201

Query: 185 MELLGMSLGVGREYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHV 244
           MELLGMSLGV R YF+ FFE+++SI RLNYYP C+ P   LGTGPHCDPTSLTILHQD V
Sbjct: 202 MELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQV 261

Query: 245 HGLQVFVDQQWHSIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLC 304
            GLQVFVD +W SIPPNP AFVVNIGDTF AL+NG YKSCLHRAVVN E  RK+ AFFLC
Sbjct: 262 GGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLC 321

Query: 305 PNEDKVVSPPSCLVD--EKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
           P  +KVV PP  LV+  +   R +PDF W   LEF+QKHYRAD  TL+ FS WL
Sbjct: 322 PKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHYRADMNTLDEFSIWL 374

BLAST of CmaCh17G000020 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 283.1 bits (723), Expect = 2.9e-76
Identity = 146/341 (42.82%), Postives = 202/341 (59.24%), Query Frame = 0

Query: 12  SLSKIPSQFIWPDHERPTLDSPELHVPPIDLKSSISDDPV-------ALSDTCR------ 71
           S   +P++F WP+ +    +  +L +P IDL   ++ +         A+   C       
Sbjct: 39  SQPNVPAEFFWPEKDVAPSEG-DLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFL 98

Query: 72  ---------LLNQAHEFMDLFFGMKLEEKQRAGRKVGELCGYANSFIGRFSCKLPWKETL 131
                    L  +A E   LFFG+  +EK RA R  G + GY      RFS  LPW ETL
Sbjct: 99  VVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETL 158

Query: 132 SFRYSAHAHSSTLVEDYFASALGEELRSSGKLYQQYCDAMSKVSLTIMELLGMSLGV-GR 191
           +  +        +VED+  S LG   +  G+++Q++CDAM+ + + +MELLG+S+G+  R
Sbjct: 159 TLAFK--KGPPHVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDR 218

Query: 192 EYFRNFFEENESIMRLNYYPPCQTPGQTLGTGPHCDPTSLTILHQDHVHGLQVFVDQQWH 251
            Y+R FFE+   I R NYYPPC+ P + LG GPH DPT++T+L QD V GL+VF    W 
Sbjct: 219 TYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQ 278

Query: 252 SIPPNPQAFVVNIGDTFTALSNGMYKSCLHRAVVNEESVRKSLAFFLCPNEDKVVSPPSC 311
           ++ P P A VVN+GDTF ALSNG Y+SC HRAVVN+E VR+SL FF CP EDK++ PP  
Sbjct: 279 TVRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPE 338

Query: 312 LVD-EKNPRIFPDFRWPTLLEFSQKHYRADDKTLEAFSNWL 329
           LV+ E+  R +PDF W  L +F+Q  YR D+ TL  FS+WL
Sbjct: 339 LVEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q391117.6e-12262.95Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1[more]
Q391101.6e-11661.90Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
O047061.2e-11159.48Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1[more]
O047053.5e-11159.48Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1[more]
O047071.8e-11059.18Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L4C75.4e-19593.85gibberellin 20 oxidase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499029 PE=3 S... [more]
A0A6J1H5L51.5e-19292.74gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460333 PE=3... [more]
A0A6J1D9N41.1e-15875.27gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111018908 PE=... [more]
S0BDY75.1e-15374.38GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox3 PE=2 SV=1[more]
A0A1S3CBV63.1e-15075.07gibberellin 20 oxidase 1-like OS=Cucumis melo OX=3656 GN=LOC103499045 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023006238.11.1e-19493.85gibberellin 20 oxidase 2-like [Cucurbita maxima][more]
KAG7013346.11.0e-19293.02Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022959323.13.0e-19292.74gibberellin 20 oxidase 2-like [Cucurbita moschata][more]
XP_023549010.11.4e-18991.83gibberellin 20 oxidase 2-like [Cucurbita pepo subsp. pepo][more]
KAG6574769.11.5e-15985.33Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G51810.15.4e-12362.95gibberellin 20 oxidase 2 [more]
AT4G25420.11.2e-11761.902-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G60980.11.6e-10657.10gibberellin 20-oxidase 4 [more]
AT5G07200.13.0e-10555.65gibberellin 20-oxidase 3 [more]
AT1G44090.12.9e-7642.82gibberellin 20-oxidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 4..309
e-value: 2.6E-73
score: 249.4
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 179..277
e-value: 3.4E-28
score: 98.1
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 13..63
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 58..329
NoneNo IPR availablePANTHERPTHR47990:SF26GIBBERELLIN 20-OXIDASEcoord: 13..63
NoneNo IPR availablePANTHERPTHR47990:SF26GIBBERELLIN 20-OXIDASEcoord: 58..329
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..317
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 177..277
score: 14.450797

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G000020.1CmaCh17G000020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009908 flower development
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009416 response to light stimulus
biological_process GO:0009826 unidimensional cell growth
molecular_function GO:0045544 gibberellin 20-oxidase activity
molecular_function GO:0046872 metal ion binding