CmaCh16G012450 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G012450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
LocationCma_Chr16: 9410986 .. 9413514 (+)
RNA-Seq ExpressionCmaCh16G012450
SyntenyCmaCh16G012450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGCATAGAGTTTCAAATCAATGGCGGAAATCCAGAATTCGGAAGCGCCAAAAATTCTCACAATGAACCAAGGAATCGGCGACCAAAGCTACGCCACAAACTCAATCTCACAGAGTAAATACCAGTCCGGATCCCGGCCTCTGCTCCGCCGCGCCATTGCTGCCTTATGCGCAGCCGATTTGCCGCCCGCCATTGCCGTTGCCGACCTCGGCTGCTCTTCAGGACCGAACGCGCTCTTCGCGATCTCCGAGATCGTCGGCGTGATCCGCGGCTGTTGCGGCGGTGAAGCGCCGGAGTTGACTGTGTTTTTGAACGATCTGGCGGAGAACGATTTCAACTCTGTTTTCAGAGGATTGTCGAACAAGAAGAACGGCGGATCGTCGGCTATGGATGAGTGTTTTGTCGCTGGAGTTCCAGGATCTTTCTATGGAAGATTGTTTGCTTCTAACAGTCTTCATTTTGTGCATTCTTCTTCCAGTCTCCATTGGCTTTCTCAGGTTAGTCTCTATTCAAATCTTCGTAATAGTTTACATTCATGTAATCATCGCAAAATAAATTTAAATTTGAAATTTAAGGATTAAAATAAATATTTGAAAGCCATTTGAATTTTACATTCTTAAACACTTTTGAATGATGATCCAATTATATTCACCCAAAGTCACTATGGATAAATATTCTCTAGAACTTCCCTACAAGTTTCTCTAGAACTTCTCCACAAAGTTTTAAAACAGAGATGTTTCCACACATATAAAAAATAGTTTGTTCTCTCCAACTTGTAGTTTTAAAACAGAGATGTTTCCATACCCATATAAAAAATGGTTTGTTCTCTTCAACCGTCGTGAGATCTCACAATCCACCCCTCTTCGAGGCGAGCGTTCTTGCTGGCACTCGTTCCCCTCTCCAATTGATGTGAGATCTCGCAATCCATCCCTTTGGAGAGCTCAGCATCCTCGCTGGCACACTACTCAGTGTCTTATTGTGAAATCGCACGTTAGTTAGAGAGGGGTATAAAGCATTATTTATAAGGGTGTGGAAACCTCTCCCGAAGTTTTAAAATCTTGAGGAAAAGTTTAGAAGGAAGAACCCAAATAGAACAATGTCTACTATAAGAGATGGGCTTGGACTATTACACAAACAGACACAAAATCTCAAAATTTAAGGGTTAAACTTATACGGTAACCTTTCATTCCGTATATCAAATCGAATAAGAACGAGGATTGATGAATGAATGAAACATGAAAGTTGAAAACAGGTACCTCAAGAGCTGAGTGAGAAAGGGAAGGGAATAAGGAACAAAGGGAAGATTTTCATATCAAAGACAAGCCCAAGTGAAGTAATAGAAGCATATTATGGTCAGTTCAAGAAGGATTTCAGTTGTTTTTTGAAGTCAAGATCAAAAGAAGTGGTGTGTGGAGGGAGAATGGTTTTAACTTTCAGAGGAAGAAGGCAGCCAGATCCATGCCCCGATGAAACTTGCCTTCTTTGGGATTACTTGGGGCTAGCCTTCCAAGCTTTGGTCCATGAGGTATATATATATTCAACCCATCTTTTTTATAATTTCGAGACTTATTCTTGCACCCGAGCTAGGTCGGAGGAAGGAAGGAGGGGAGCCAGTGTCCCAACCCCTCTCTAATTGTCTTAGATAGTCGTCCTTAAACTTTCAAGTTTGTGTTCATTTGGTCACTGAATTTTTTAAAACACCAAATAACTACTCCTTTAAGATTCAATTATGCACCAAATATGCTCGTCAACATAAAATTCGATCAGTTAATTTTTAAAAATTGTGAAAAAAATGTCGAAAACTAGATAGAAAAGTAAAAGTTTAGGGACTTATAGACATCTTTAGAAACTTTACGAACCGAAATAAGATAATTTTCTTCGTCGAGTAACCAAATTATTTATATGTTTATTTAGGGGCTTATTGAGGAGGAGAAAGTAGATAGCTACAACACTCCTTATTATGAACCGTATGTGGAGGATGTGAGAAGGGAGATAGAGGAAGAAGGGTCCTTCAAAATAGAGAATTTGGAGATAATTGCTTTGGCATGGGATGGTGTGAATAGAGATGGGGAAAGCTATGAACGATCAAAGACAACCCAACAAATGGCGAAGGCCATTCAAGCTGTGAATGAATGTATGATAAGAGCCCATTTTGGAGGAGATATTATAGAGTCATTGTTTAAGAGATTTGAAGAAATCATGGTGGCTGATACTAGAGAGGTGGAGCATGTTAGTTTGGTTCTTTCTTTGCTTAGAAAATAATGACAATATTCTTATCAACGTTACCATCTTGTTAGCGTTTTAGGGTTTCGATGGGTATAAACGAGTTGTCGAGTCTTGAAGTTTTGTTAGATTTCGATGGGTATATACGAGTTGTCGAATTTTGAAGTTTTGTTGGATTTCGATGGGTACAAACAAATTGTCGAGTCTGAAAGTTTTGTTGGGTTTCGATGGGTATAAACGAGTTGTCAAGTTTGGATGAGCATAAACGAATGGTCGAGTCTGAGAGTTTTGTTGGGTTTCGATGG

mRNA sequence

AGAGCATAGAGTTTCAAATCAATGGCGGAAATCCAGAATTCGGAAGCGCCAAAAATTCTCACAATGAACCAAGGAATCGGCGACCAAAGCTACGCCACAAACTCAATCTCACAGAGTAAATACCAGTCCGGATCCCGGCCTCTGCTCCGCCGCGCCATTGCTGCCTTATGCGCAGCCGATTTGCCGCCCGCCATTGCCGTTGCCGACCTCGGCTGCTCTTCAGGACCGAACGCGCTCTTCGCGATCTCCGAGATCGTCGGCGTGATCCGCGGCTGTTGCGGCGGTGAAGCGCCGGAGTTGACTGTGTTTTTGAACGATCTGGCGGAGAACGATTTCAACTCTGTTTTCAGAGGATTGTCGAACAAGAAGAACGGCGGATCGTCGGCTATGGATGAGTGTTTTGTCGCTGGAGTTCCAGGATCTTTCTATGGAAGATTGTTTGCTTCTAACAGTCTTCATTTTGTGCATTCTTCTTCCAGTCTCCATTGGCTTTCTCAGGTACCTCAAGAGCTGAGTGAGAAAGGGAAGGGAATAAGGAACAAAGGGAAGATTTTCATATCAAAGACAAGCCCAAGTGAAGTAATAGAAGCATATTATGGTCAGTTCAAGAAGGATTTCAGTTGTTTTTTGAAGTCAAGATCAAAAGAAGTGGTGTGTGGAGGGAGAATGGTTTTAACTTTCAGAGGAAGAAGGCAGCCAGATCCATGCCCCGATGAAACTTGCCTTCTTTGGGATTACTTGGGGCTAGCCTTCCAAGCTTTGGTCCATGAGGGGCTTATTGAGGAGGAGAAAGTAGATAGCTACAACACTCCTTATTATGAACCGTATGTGGAGGATGTGAGAAGGGAGATAGAGGAAGAAGGGTCCTTCAAAATAGAGAATTTGGAGATAATTGCTTTGGCATGGGATGGTGTGAATAGAGATGGGGAAAGCTATGAACGATCAAAGACAACCCAACAAATGGCGAAGGCCATTCAAGCTGTGAATGAATGTATGATAAGAGCCCATTTTGGAGGAGATATTATAGAGTCATTGTTTAAGAGATTTGAAGAAATCATGGTGGCTGATACTAGAGAGGTGGAGCATGTTAGTTTGGTTCTTTCTTTGCTTAGAAAATAATGACAATATTCTTATCAACGTTACCATCTTGTTAGCGTTTTAGGGTTTCGATGGGTATAAACGAGTTGTCGAGTCTTGAAGTTTTGTTAGATTTCGATGGGTATATACGAGTTGTCGAATTTTGAAGTTTTGTTGGATTTCGATGGGTACAAACAAATTGTCGAGTCTGAAAGTTTTGTTGGGTTTCGATGGGTATAAACGAGTTGTCAAGTTTGGATGAGCATAAACGAATGGTCGAGTCTGAGAGTTTTGTTGGGTTTCGATGG

Coding sequence (CDS)

ATGGCGGAAATCCAGAATTCGGAAGCGCCAAAAATTCTCACAATGAACCAAGGAATCGGCGACCAAAGCTACGCCACAAACTCAATCTCACAGAGTAAATACCAGTCCGGATCCCGGCCTCTGCTCCGCCGCGCCATTGCTGCCTTATGCGCAGCCGATTTGCCGCCCGCCATTGCCGTTGCCGACCTCGGCTGCTCTTCAGGACCGAACGCGCTCTTCGCGATCTCCGAGATCGTCGGCGTGATCCGCGGCTGTTGCGGCGGTGAAGCGCCGGAGTTGACTGTGTTTTTGAACGATCTGGCGGAGAACGATTTCAACTCTGTTTTCAGAGGATTGTCGAACAAGAAGAACGGCGGATCGTCGGCTATGGATGAGTGTTTTGTCGCTGGAGTTCCAGGATCTTTCTATGGAAGATTGTTTGCTTCTAACAGTCTTCATTTTGTGCATTCTTCTTCCAGTCTCCATTGGCTTTCTCAGGTACCTCAAGAGCTGAGTGAGAAAGGGAAGGGAATAAGGAACAAAGGGAAGATTTTCATATCAAAGACAAGCCCAAGTGAAGTAATAGAAGCATATTATGGTCAGTTCAAGAAGGATTTCAGTTGTTTTTTGAAGTCAAGATCAAAAGAAGTGGTGTGTGGAGGGAGAATGGTTTTAACTTTCAGAGGAAGAAGGCAGCCAGATCCATGCCCCGATGAAACTTGCCTTCTTTGGGATTACTTGGGGCTAGCCTTCCAAGCTTTGGTCCATGAGGGGCTTATTGAGGAGGAGAAAGTAGATAGCTACAACACTCCTTATTATGAACCGTATGTGGAGGATGTGAGAAGGGAGATAGAGGAAGAAGGGTCCTTCAAAATAGAGAATTTGGAGATAATTGCTTTGGCATGGGATGGTGTGAATAGAGATGGGGAAAGCTATGAACGATCAAAGACAACCCAACAAATGGCGAAGGCCATTCAAGCTGTGAATGAATGTATGATAAGAGCCCATTTTGGAGGAGATATTATAGAGTCATTGTTTAAGAGATTTGAAGAAATCATGGTGGCTGATACTAGAGAGGTGGAGCATGTTAGTTTGGTTCTTTCTTTGCTTAGAAAATAA

Protein sequence

MAEIQNSEAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLPPAIAVADLGCSSGPNALFAISEIVGVIRGCCGGEAPELTVFLNDLAENDFNSVFRGLSNKKNGGSSAMDECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDPCPDETCLLWDYLGLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGESYERSKTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRFEEIMVADTREVEHVSLVLSLLRK
Homology
BLAST of CmaCh16G012450 vs. ExPASy Swiss-Prot
Match: A0A061DFA8 (Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 PE=3 SV=2)

HSP 1 Score: 421.0 bits (1081), Expect = 1.4e-116
Identity = 217/362 (59.94%), Postives = 266/362 (73.48%), Query Frame = 0

Query: 11  KILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLPPAIAVADLGCSSGPN 70
           +IL+MN G G+QSYA NSI QS   S S P+L++A+   C  +LP  + +ADLGCSSGPN
Sbjct: 3   QILSMNGGSGEQSYANNSIGQSTCLSRSMPVLKQAVLDSCCTNLPETVTMADLGCSSGPN 62

Query: 71  ALFAISEIVGVI-RGCC--GGEAPELTVFLNDLAENDFNSVFRGL----SNKKNGGSSAM 130
             +A+SEI  +I + CC  G  APE  VFLNDL  NDFN+VF+ L       +       
Sbjct: 63  TFYAVSEITSIIYKRCCQLGRSAPEFRVFLNDLPGNDFNTVFQSLPAYQGKLREENGPGF 122

Query: 131 DECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTS 190
              +VAGVPGSFYGRLF   +LHFVHSSSSLHWLSQVP EL++K   + NK KIFISKTS
Sbjct: 123 GPLYVAGVPGSFYGRLFPLKTLHFVHSSSSLHWLSQVPPELTDKANPLINKWKIFISKTS 182

Query: 191 PSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDPCPDETCLLWDYLGLA 250
           P  VI +Y  QF+KDFS FLK RS+EV   GRMVLTFRGR   DP  DE+CLLWDYLG A
Sbjct: 183 PPAVISSYLTQFRKDFSLFLKLRSEEVAPEGRMVLTFRGRTTADPTSDESCLLWDYLGQA 242

Query: 251 FQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGE 310
           FQ LV EGL+EEE++D+YNTPYYEP+ E+++ EIE+EGSF ++ LE+IAL WD VN  G 
Sbjct: 243 FQDLVAEGLVEEERLDTYNTPYYEPHPEEIQAEIEKEGSFTLDRLEVIALPWDSVN-GGI 302

Query: 311 SYERSKTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRFEEIMVADTREVEHVSLVLSLL 366
             +R+ T ++MAKAI+AVNE M R+HFG DI++ LF RF EIM ADT+EVEHVSLV+SL+
Sbjct: 303 KDDRATTAKRMAKAIRAVNESMFRSHFGEDILDPLFHRFIEIMAADTKEVEHVSLVISLI 362

BLAST of CmaCh16G012450 vs. ExPASy Swiss-Prot
Match: A0A061FBW2 (Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 GN=TCM_034091 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.1e-85
Identity = 175/372 (47.04%), Postives = 240/372 (64.52%), Query Frame = 0

Query: 8   EAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLPPAIAVADLGCSS 67
           E  ++L MN+G G+ SYA NS  QSK  S  +P++  A+  +   +L  ++ +ADLGCSS
Sbjct: 2   EVMQVLHMNKGNGETSYAKNSTVQSKIISVGKPIIEEAVHEISCNNLLESMGIADLGCSS 61

Query: 68  GPNALFAISEIVGVIRGC---CGGEAPELTVFLNDLAENDFNSVFRGL----SNKKNGGS 127
           GPN L  ISEI+ +++      G   PE  V+LNDL  NDFN +F  L       K    
Sbjct: 62  GPNTLSVISEIMDMVQTTSRRLGRPVPEFRVYLNDLYSNDFNYIFMSLPAFYHRLKEEKG 121

Query: 128 SAMDECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFIS 187
                C+++GV GSFYGRLF S SLHFVHSSSSLHWLSQVP  L  K     NKGK++IS
Sbjct: 122 IGCGSCYISGVAGSFYGRLFPSKSLHFVHSSSSLHWLSQVPPGLESKALAPLNKGKVYIS 181

Query: 188 KTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDPCPDETCLLWDYL 247
           K+SP  V+ AY  QF+ DFS F++SRS+E+V GGRMVL+F GRR  DP  +E+C  W+ L
Sbjct: 182 KSSPQSVLNAYSLQFQNDFSMFIESRSQELVPGGRMVLSFMGRRSTDPTTEESCHHWELL 241

Query: 248 GLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDG--V 307
             A  +LV EGLIEE KVDS+N PYY P  E+++ EI++ GSF I+ LE   + WDG  V
Sbjct: 242 AQAIMSLVREGLIEEAKVDSFNAPYYAPCAEEIKVEIQKVGSFVIDRLEGFEIDWDGGAV 301

Query: 308 NRDGESYERSKTTQQMAKAIQAVNECMIRAHF--GGDIIESLFKRFEEIM---VADTREV 366
           +    +  +    Q++AK I+AV E M+ +HF  G DI++ LF R+ EI+   ++ TR  
Sbjct: 302 SDVQTAQGKLLIGQRVAKTIRAVVESMLESHFGIGQDIMDDLFSRYAEIVGNHLSKTR-T 361

BLAST of CmaCh16G012450 vs. ExPASy Swiss-Prot
Match: A0A061FDP1 (Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 GN=TCM_034089 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 8.1e-85
Identity = 174/372 (46.77%), Postives = 239/372 (64.25%), Query Frame = 0

Query: 8   EAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLPPAIAVADLGCSS 67
           E  ++L MN+G G+ SYA NS  QSK  S  +P++  A+  +   ++  ++ +ADLGCSS
Sbjct: 2   EVMQVLHMNRGNGENSYAKNSTVQSKIISIGKPIIEEAVHEISCNNVLESMGIADLGCSS 61

Query: 68  GPNALFAISEIVGVIRGC---CGGEAPELTVFLNDLAENDFNSVFRGL----SNKKNGGS 127
           GPN L  ISEI+ +++      G   PE  ++LNDL  NDFNS+F  L       K    
Sbjct: 62  GPNTLCVISEIMDMVQATSHRLGHPVPEFRLYLNDLYSNDFNSIFMSLPAFYHRLKEEKG 121

Query: 128 SAMDECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFIS 187
                CF++GV GSFYGRLF S SLH+VHSSSSLHWLSQVP  L        NKGK++IS
Sbjct: 122 IGCGSCFISGVAGSFYGRLFPSKSLHYVHSSSSLHWLSQVPPGLESNAVTPLNKGKVYIS 181

Query: 188 KTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDPCPDETCLLWDYL 247
           K+SP  V+ AY  QF+ DF  F++SRS+E+V GGRMVL+  GRR  DP  +E+C  W+ L
Sbjct: 182 KSSPHSVLHAYSLQFQNDFPMFIESRSQELVSGGRMVLSLFGRRSTDPTTEESCYQWELL 241

Query: 248 GLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDG--V 307
             A  +LV EGLIEEEKVDS+NTP+Y P  E+++ EI++EGSF I+ LE   + WDG  V
Sbjct: 242 AQAIMSLVREGLIEEEKVDSFNTPFYAPCAEEIKVEIQKEGSFIIDRLEGFEIDWDGGAV 301

Query: 308 NRDGESYERSKTTQQMAKAIQAVNECMIRAHF--GGDIIESLFKRFEEIM---VADTREV 366
           +    +  +    Q+ AKA++AV E M+ +HF  G DI++ LF R+ EI+   ++ TR  
Sbjct: 302 SDVHTAQGKLLIGQRAAKALRAVVESMLESHFGIGQDIMDDLFSRYAEIVGNHLSKTR-T 361

BLAST of CmaCh16G012450 vs. ExPASy Swiss-Prot
Match: Q9AR07 (Jasmonate O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=JMT PE=1 SV=3)

HSP 1 Score: 291.2 bits (744), Expect = 1.6e-77
Identity = 173/389 (44.47%), Postives = 238/389 (61.18%), Query Frame = 0

Query: 8   EAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLP-PAIAVADLGCS 67
           E  ++L MN+G G+ SYA NS +QS   S  R ++  A+  L  ++    +I +ADLGCS
Sbjct: 2   EVMRVLHMNKGNGETSYAKNSTAQSNIISLGRRVMDEALKKLMMSNSEISSIGIADLGCS 61

Query: 68  SGPNALFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGL-------SNKK 127
           SGPN+L +IS IV  I   C       PEL V LNDL  NDFN +   L       +N K
Sbjct: 62  SGPNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNNK 121

Query: 128 NG---GSSAMDECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEK-----G 187
            G   G    + CFV+ VPGSFYGRLF   SLHFVHSSSSLHWLSQVP   +EK      
Sbjct: 122 EGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTIT 181

Query: 188 KGIRNKGKIFISKTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDP 247
             + N GKI+ISKTSP    +AY  QF+ DF  FL+SRS+E+V GGRMVL+F GRR  DP
Sbjct: 182 ADLENMGKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDP 241

Query: 248 CPDETCLLWDYLGLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENL 307
             +E+C  W+ L  A  ++  EG+IEEEK+D++N PYY    E+++  IE+EGSF I+ L
Sbjct: 242 TTEESCYQWELLAQALMSMAKEGIIEEEKIDAFNAPYYAASSEELKMVIEKEGSFSIDRL 301

Query: 308 EIIALAWDGVNRDGESYE---RSK-----TTQQMAKAIQAVNECMIRAHFGGDIIESLFK 365
           EI  + W+G +   ESY+   RSK     + ++++  I+AV E M+   FG ++++ LF+
Sbjct: 302 EISPIDWEGGSISEESYDLVIRSKPEALASGRRVSNTIRAVVEPMLEPTFGENVMDELFE 361

BLAST of CmaCh16G012450 vs. ExPASy Swiss-Prot
Match: Q9SBK6 (Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis OX=51351 GN=JMT PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.2e-75
Identity = 169/392 (43.11%), Postives = 233/392 (59.44%), Query Frame = 0

Query: 8   EAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLP-PAIAVADLGCS 67
           E  +IL MN+G G+ SYA NSI QS   S  R ++  A+  L   +    +  +ADLGCS
Sbjct: 2   EVMRILHMNKGNGETSYAKNSIVQSNIISLGRRVMDEALKKLMIRNSEILSFGIADLGCS 61

Query: 68  SGPNALFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGLS------NKKN 127
           SGPN+L +IS IV  I+  C       PEL++ LNDL  NDFN +F  L        K++
Sbjct: 62  SGPNSLLSISNIVETIQNLCHDLDRPVPELSLSLNDLPSNDFNYIFASLPEFYDRVKKRD 121

Query: 128 GGSSAM-------DECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKG 187
               ++         CFV+ VPGSFYGRLF   SLHFVHSSSSLHWLSQVP     K  G
Sbjct: 122 NNYESLGFEHGSGGPCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQVPCGEVNKKDG 181

Query: 188 I------RNKGKIFISKTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRR 247
           +       N+GKI++SKTSP    + Y  QF+ DFS FL+SRS+E+V GGRMVL+F GR 
Sbjct: 182 VVITADLDNRGKIYLSKTSPKSAHKVYALQFQTDFSVFLRSRSEELVPGGRMVLSFLGRS 241

Query: 248 QPDPCPDETCLLWDYLGLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFK 307
            PDP  +E+C  W+ L  A  +L  EG+IEEE +D++N PYY    E+++  IE+EGSF 
Sbjct: 242 SPDPTTEESCYQWELLAQALMSLAKEGIIEEENIDAFNAPYYAASPEELKMAIEKEGSFS 301

Query: 308 IENLEIIALAWDGVNRDGESY-------ERSKTTQQMAKAIQAVNECMIRAHFGGDIIES 365
           I+ LEI  + W+G +   +SY       E   + +++AK I+AV E M+   FG  +++ 
Sbjct: 302 IDRLEISPVDWEGGSISDDSYDIVRFKPEALASGRRVAKTIRAVVEPMLEPTFGQKVMDE 361

BLAST of CmaCh16G012450 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-78
Identity = 173/389 (44.47%), Postives = 238/389 (61.18%), Query Frame = 0

Query: 8   EAPKILTMNQGIGDQSYATNSISQSKYQSGSRPLLRRAIAALCAADLP-PAIAVADLGCS 67
           E  ++L MN+G G+ SYA NS +QS   S  R ++  A+  L  ++    +I +ADLGCS
Sbjct: 2   EVMRVLHMNKGNGETSYAKNSTAQSNIISLGRRVMDEALKKLMMSNSEISSIGIADLGCS 61

Query: 68  SGPNALFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGL-------SNKK 127
           SGPN+L +IS IV  I   C       PEL V LNDL  NDFN +   L       +N K
Sbjct: 62  SGPNSLLSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNNK 121

Query: 128 NG---GSSAMDECFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEK-----G 187
            G   G    + CFV+ VPGSFYGRLF   SLHFVHSSSSLHWLSQVP   +EK      
Sbjct: 122 EGLGFGRGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTIT 181

Query: 188 KGIRNKGKIFISKTSPSEVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPDP 247
             + N GKI+ISKTSP    +AY  QF+ DF  FL+SRS+E+V GGRMVL+F GRR  DP
Sbjct: 182 ADLENMGKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDP 241

Query: 248 CPDETCLLWDYLGLAFQALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENL 307
             +E+C  W+ L  A  ++  EG+IEEEK+D++N PYY    E+++  IE+EGSF I+ L
Sbjct: 242 TTEESCYQWELLAQALMSMAKEGIIEEEKIDAFNAPYYAASSEELKMVIEKEGSFSIDRL 301

Query: 308 EIIALAWDGVNRDGESYE---RSK-----TTQQMAKAIQAVNECMIRAHFGGDIIESLFK 365
           EI  + W+G +   ESY+   RSK     + ++++  I+AV E M+   FG ++++ LF+
Sbjct: 302 EISPIDWEGGSISEESYDLVIRSKPEALASGRRVSNTIRAVVEPMLEPTFGENVMDELFE 361

BLAST of CmaCh16G012450 vs. TAIR 10
Match: AT5G66430.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 8.3e-69
Identity = 155/364 (42.58%), Postives = 215/364 (59.07%), Query Frame = 0

Query: 15  MNQGIGDQSYATNSISQSKYQSGSRP-LLRRAIAALCAADLPPAIAVADLGCSSGPNALF 74
           M+ G GD SY+TNS+ Q K  S ++P L++     +   + P  I VADLGC++G N   
Sbjct: 1   MSGGDGDNSYSTNSLLQKKVLSKAKPVLVKNTKGMMINLNFPNYIKVADLGCATGENTFL 60

Query: 75  AISEIVGVIRGC---CGGEAPELTVFLNDLAENDFNSVFRGLS--NKKNGGSSAMDECFV 134
            ++EIV  I      C  + PE+   LNDL +NDFN+ F+ +   NK+     +   CFV
Sbjct: 61  TMAEIVNTINVLCQQCNQKPPEIDCCLNDLPDNDFNTTFKFVPFFNKR---VKSKRLCFV 120

Query: 135 AGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTSPSEVI 194
           +GVPGSFY RLF   SLHFVHSS SLHWLS+VP+ L       +N   ++I+ +SP    
Sbjct: 121 SGVPGSFYSRLFPRKSLHFVHSSYSLHWLSKVPKGLE------KNSSSVYITTSSPPNAY 180

Query: 195 EAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQ-PDPCPDETCLLWDYLGLAFQAL 254
           +AY  QF+ DF  FL+ RS+E+V  GRMVLTF GR+   DP   + C  W  L  + + L
Sbjct: 181 KAYLNQFQSDFKSFLEMRSEEMVSNGRMVLTFIGRKTLDDPLHRDCCHFWTLLSTSLRDL 240

Query: 255 VHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGESY-- 314
           V+EGL+   KVDS+N P+Y+P  E+V   I  EGSF+I +LEI      G++   E Y  
Sbjct: 241 VYEGLVSASKVDSFNIPFYDPSKEEVMEMIRNEGSFEINDLEIHGFEL-GLSNHDEDYML 300

Query: 315 --ERSKTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRF--EEIMVADTREVEHVSLVLS 366
             + SK  Q+ A  I+AV+E M+ A FG DI+++LFK+F       A       V+LV+S
Sbjct: 301 HSQISKAGQREANCIRAVSESMLVADFGVDIMDTLFKKFAYHVSQHASCTNKTTVTLVVS 354

BLAST of CmaCh16G012450 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 4.1e-68
Identity = 153/365 (41.92%), Postives = 214/365 (58.63%), Query Frame = 0

Query: 11  KILTMNQGIGDQSYATNSISQSKYQSGSRP-LLRRAIAALCAADLPPAIAVADLGCSSGP 70
           K L M+ G G  SY+ NS  Q K  S ++P L+R     +   D P  I VA+LGCSSG 
Sbjct: 27  KALCMSGGDGANSYSANSRLQKKVLSMAKPVLVRNTEEMMMNLDFPTYIKVAELGCSSGQ 86

Query: 71  NALFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGLS-NKKNGGSSAMDE 130
           N+  AI EI+  I   C      +PE+   LNDL ENDFN+ F+ +    K    +    
Sbjct: 87  NSFLAIFEIINTINVLCQHVNKNSPEIDCCLNDLPENDFNTTFKFVPFFNKELMITNKSS 146

Query: 131 CFVAGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTSPS 190
           CFV G PGSFY RLF+ NSLH +HSS +LHWLS+VP++L        NKG ++I+ +SP 
Sbjct: 147 CFVYGAPGSFYSRLFSRNSLHLIHSSYALHWLSKVPEKLE------NNKGNLYITSSSPQ 206

Query: 191 EVIEAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQ-PDPCPDETCLLWDYLGLAF 250
              +AY  QF+KDF+ FL+ RS+E+V  GRMVLTF GR    DP   + C  W  L  + 
Sbjct: 207 SAYKAYLNQFQKDFTMFLRLRSEEIVSNGRMVLTFIGRNTLNDPLYRDCCHFWTLLSNSL 266

Query: 251 QALVHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGES 310
           + LV EGL+ E K+D++N P+Y+P V++++  I++EGSF+I  LE            G  
Sbjct: 267 RDLVFEGLVSESKLDAFNMPFYDPNVQELKEVIQKEGSFEINELESHGFDL------GHY 326

Query: 311 YERS--KTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRFEEIMV--ADTREVEHVSLVL 366
           YE    +  +  A  I+AV+E M+ AHFG +II++LF ++   +   A+ R    VSLV+
Sbjct: 327 YEEDDFEAGRNEANGIRAVSEPMLIAHFGEEIIDTLFDKYAYHVTQHANCRNKTTVSLVV 379

BLAST of CmaCh16G012450 vs. TAIR 10
Match: AT5G04370.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 147/360 (40.83%), Postives = 206/360 (57.22%), Query Frame = 0

Query: 13  LTMNQGIGDQSYATNSISQSKYQSGSRP-LLRRAIAALCAADLPPAIAVADLGCSSGPNA 72
           L M  G G  SY++NS+ Q +  S ++P L++     +   + P  I VADLGCSSG N 
Sbjct: 39  LCMRGGDGYNSYSSNSLLQRRVLSKAKPVLVKNTKDLMINLNFPTYIKVADLGCSSGQNT 98

Query: 73  LFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGLSNKKNGGSSAMDECFV 132
             A+SEI+  I   C       PE+   LNDL  NDFN+ F+ +        ++ +  FV
Sbjct: 99  FLAMSEIINTINVFCQQRNQNPPEIDCCLNDLPSNDFNTTFKFIQFFNGMNITSKESYFV 158

Query: 133 AGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTSPSEVI 192
            GVPGSFY RLF   SLHFVHSS  LHWLS+VP+ L       +NK  ++I+ +SP    
Sbjct: 159 YGVPGSFYSRLFPRRSLHFVHSSYGLHWLSKVPEGLE------KNKMSVYITNSSPLSTY 218

Query: 193 EAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPD-PCPDETCLLWDYLGLAFQAL 252
           +AY  QF++DF+ FLK RS+E+V  GRMVLTF GR   D P   + C  W  L  + + L
Sbjct: 219 KAYLNQFQRDFATFLKLRSEEMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDL 278

Query: 253 VHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGESYER 312
           V EGL+   KVDS+  P+Y+P  ++++  +++EGSF+I +LE         N+D    +R
Sbjct: 279 VAEGLVSASKVDSFYLPFYDPNEKEIKEMVQKEGSFEIRDLETHGYDLGHCNQD--ESKR 338

Query: 313 SKTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRF--EEIMVADTREVEHVSLVLSLLRK 366
           SK+ Q  A  I+AV+E ++ AHFG  II  LF +F          R    VS+V+SL +K
Sbjct: 339 SKSGQNEANYIRAVSEPLLAAHFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSLTKK 390

BLAST of CmaCh16G012450 vs. TAIR 10
Match: AT5G04370.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-65
Identity = 147/360 (40.83%), Postives = 206/360 (57.22%), Query Frame = 0

Query: 13  LTMNQGIGDQSYATNSISQSKYQSGSRP-LLRRAIAALCAADLPPAIAVADLGCSSGPNA 72
           L M  G G  SY++NS+ Q +  S ++P L++     +   + P  I VADLGCSSG N 
Sbjct: 58  LCMRGGDGYNSYSSNSLLQRRVLSKAKPVLVKNTKDLMINLNFPTYIKVADLGCSSGQNT 117

Query: 73  LFAISEIVGVIRGCC---GGEAPELTVFLNDLAENDFNSVFRGLSNKKNGGSSAMDECFV 132
             A+SEI+  I   C       PE+   LNDL  NDFN+ F+ +        ++ +  FV
Sbjct: 118 FLAMSEIINTINVFCQQRNQNPPEIDCCLNDLPSNDFNTTFKFIQFFNGMNITSKESYFV 177

Query: 133 AGVPGSFYGRLFASNSLHFVHSSSSLHWLSQVPQELSEKGKGIRNKGKIFISKTSPSEVI 192
            GVPGSFY RLF   SLHFVHSS  LHWLS+VP+ L       +NK  ++I+ +SP    
Sbjct: 178 YGVPGSFYSRLFPRRSLHFVHSSYGLHWLSKVPEGLE------KNKMSVYITNSSPLSTY 237

Query: 193 EAYYGQFKKDFSCFLKSRSKEVVCGGRMVLTFRGRRQPD-PCPDETCLLWDYLGLAFQAL 252
           +AY  QF++DF+ FLK RS+E+V  GRMVLTF GR   D P   + C  W  L  + + L
Sbjct: 238 KAYLNQFQRDFATFLKLRSEEMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDL 297

Query: 253 VHEGLIEEEKVDSYNTPYYEPYVEDVRREIEEEGSFKIENLEIIALAWDGVNRDGESYER 312
           V EGL+   KVDS+  P+Y+P  ++++  +++EGSF+I +LE         N+D    +R
Sbjct: 298 VAEGLVSASKVDSFYLPFYDPNEKEIKEMVQKEGSFEIRDLETHGYDLGHCNQD--ESKR 357

Query: 313 SKTTQQMAKAIQAVNECMIRAHFGGDIIESLFKRF--EEIMVADTREVEHVSLVLSLLRK 366
           SK+ Q  A  I+AV+E ++ AHFG  II  LF +F          R    VS+V+SL +K
Sbjct: 358 SKSGQNEANYIRAVSEPLLAAHFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSLTKK 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A061DFA81.4e-11659.94Probable methyltransferase TCM_000168 OS=Theobroma cacao OX=3641 GN=TCM_000168 P... [more]
A0A061FBW22.1e-8547.04Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 G... [more]
A0A061FDP18.1e-8546.77Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 G... [more]
Q9AR071.6e-7744.47Jasmonate O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=JMT PE=1 SV=3[more]
Q9SBK61.2e-7543.11Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis OX=51351 GN=JMT... [more]
Match NameE-valueIdentityDescription
AT1G19640.11.2e-7844.47jasmonic acid carboxyl methyltransferase [more]
AT5G66430.18.3e-6942.58S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G11480.14.1e-6841.92S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G04370.11.9e-6540.83S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G04370.21.9e-6540.83S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 13..350
e-value: 2.9E-123
score: 413.0
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 27..294
e-value: 2.9E-123
score: 413.0
NoneNo IPR availablePANTHERPTHR31009:SF18JASMONATE O-METHYLTRANSFERASE-LIKEcoord: 11..363
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 46..363
e-value: 1.0E-113
score: 380.0
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 11..363
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 11..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G012450.1CmaCh16G012450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity