CmaCh16G009330 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009330
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectin lyase-like superfamily protein, putative
Location	Cma_Chr16: 7133136 .. 7134902 (-)
RNA-Seq Expression	CmaCh16G009330
Synteny	CmaCh16G009330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTTTGATATCATCCAATTTGGTGCAACATCCGATGGAAAAATGGATAGCGCGAAGGTAAAATTATATACTCGATTCAAACAACTTTTGTTCATTATTTACAATATTAGTATATGTTTCATAGGCAACATTTTTATTTTTTATTTTATATTCTTTTAAAAGTTTTTGAAAAATAGAACGCATTAGAAACATTATTTTAAAAATACCATTTTTCAAATGGGATACAATTTTTGGAAACAAGGCCATGAGTTTCTTAAGCCTTTTTGGTTTTTTCTTTGTTTTATTATCTATTTCAGGTCTATAAAACAAATTTTTAAAACTAAAAAATTATATATTTTTTGTTTTTTGATAATTCATCAGCATAATAAATATCTAAACGAAAATAATAATGTAAAAATAATTTTTATATTATTTATGGTAAATTCAGTCGCACACTAAAAAAAATTTAAAAATTTCCAAAAATAATAAAATTTAAAATTTAAGGTATTTAGTTGAAAGTAGATTAAGTTATTTAGTTTGTATAGGCATTAAGTAGCGCATGGAGGGAAGCTTGTGGACATAATGGAGGTGGTGTAGTTTTAGAGCCGATGTTCCTTTTCGAAGAGTAGAATCGAAGTATAGTGTTGAACAGGTCGGTGAAGGGAGGTTTCTAGTTTTGCCGTTGGTGCTTGAAGGTCCATGTAAAGGATCCATTGGTCTGGATATTAAAGGTGAACTGTTGGCTCCAGTTGCCAAGAATTTCTCCATCGGTGAATATTGGCTAAGTATTTATCAAGTGGACAACTTTTTTGTTGACGGTCCTCGCAGCTTGAACGGCGGTGGTTCTTCAGCTTGGTCTTCCAACACTTCTAAACGTCCAATTGTAAGTCTATTTTGAGGTTTAAATACTAAAAAAAAGTTGATTGGTTAGGTTGCAGTTAAATGGATGCAATCATTTTACATTTATTTTAAATGTAAACCAAAATATACTTGTGCATGATATAATGTCAACACAGCGAGAATTGCAAAGATAACATCGTCCAATAGCAAGTTGTTCCATTTTGCTGTGCATGGATGTCGTGATGTCACGTTTGATCACGTTACAGTCCCAATACAGATGGAATTCACGTTTCAAACTCAAGTGGTGTCAACATTATGCATTCAGTGATTGACACTGGAGACGATTGTATCTCTTTGGGCCCGGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGGTATATATATTCAAATTTGATATCGTTAGGTTTTAGTAAAAGTGAAAATGGTATTACAATGAATGTTGTTTGTGGTGGACAGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTT

mRNA sequence

CGTTTGATATCATCCAATTTGGTGCAACATCCGATGGAAAAATGGATAGCGCGAAGGCATTAAGTAGCGCATGGAGGGAAGCTTGTGGACATAATGGAGGTGGTGTAGTTTTAGAGCCGATAGTAGAATCGAAGTATAGTGTTGAACAGGTCGGTGAAGGGAGGTTTCTAGTTTTGCCGTTGGTGCTTGAAGGTCCATGTAAAGGATCCATTGGTCTGGATATTAAAGGTGAACTGTTGGCTCCAGTTGCCAAGAATTTCTCCATCGGTGAATATTGGCTAAGTATTTATCAAGTGGACAACTTTTTTGTTGACGGTCCTCGCAGCTTGAACGGCGGTGGTTCTTCAGCTTGGTCTTCCAACACTTCTAAACGTCCAATTGTAACGAGAATTGCAAAGATAACATCGTCCAATAGCAAGTTGTTCCATTTTGCTGTGCATGGATGTCGTGATGTCACTCCCAATACAGATGGAATTCACGTTTCAAACTCAAGTGGTGTCAACATTATGCATTCAGTGATTGACACTGGAGACGATTGTATCTCTTTGGGCCCGGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTT

Coding sequence (CDS)

ATGGATAGCGCGAAGGCATTAAGTAGCGCATGGAGGGAAGCTTGTGGACATAATGGAGGTGGTGTAGTTTTAGAGCCGATAGTAGAATCGAAGTATAGTGTTGAACAGGTCGGTGAAGGGAGGTTTCTAGTTTTGCCGTTGGTGCTTGAAGGTCCATGTAAAGGATCCATTGGTCTGGATATTAAAGGTGAACTGTTGGCTCCAGTTGCCAAGAATTTCTCCATCGGTGAATATTGGCTAAGTATTTATCAAGTGGACAACTTTTTTGTTGACGGTCCTCGCAGCTTGAACGGCGGTGGTTCTTCAGCTTGGTCTTCCAACACTTCTAAACGTCCAATTGTAACGAGAATTGCAAAGATAACATCGTCCAATAGCAAGTTGTTCCATTTTGCTGTGCATGGATGTCGTGATGTCACTCCCAATACAGATGGAATTCACGTTTCAAACTCAAGTGGTGTCAACATTATGCATTCAGTGATTGACACTGGAGACGATTGTATCTCTTTGGGCCCGGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTT

Protein sequence

MDSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDIKGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWSSNTSKRPIVTRIAKITSSNSKLFHFAVHGCRDVTPNTDGIHVSNSSGVNIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQLPSSCL

Homology

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BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 8.6e-73
Identity = 149/366 (40.71%), Postives = 211/366 (57.65%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           DS +AL++AW+EAC       +L P            +G F V  + LEGPCK SIGL +
Sbjct: 1   DSTQALTTAWKEACASASPSTILVP------------KGNFAVGLITLEGPCKSSIGLQL 60

Query: 62  KGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWSSNTSKR--PIVTRIA- 121
           +G L AP   +   G  W+++ ++D   + G    +G G SAW  N   +  PI   ++ 
Sbjct: 61  QGTLKAPADPSKIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSM 120

Query: 122 -------------KITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSGV 181
                         +T+ +SK FH  V GC+++T             NTDGIH+  S GV
Sbjct: 121 NLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGV 180

Query: 182 NIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTF 241
           NI+++ I TGDDCISLG GS+ INITNI CGPGHGIS+GSLG+Y NEE V  + V++ T 
Sbjct: 181 NIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTI 240

Query: 242 VGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIR 301
            G+ NGVRIKTW  S P   +++ F ++ M  V  P++IDQ YC          S V++ 
Sbjct: 241 TGSQNGVRIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLS 300

Query: 302 DVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGK 339
            + ++ I+GTS T+ AV   CS+ FPC G+ L DI+L+++G GG  TS+C N+K +  GK
Sbjct: 301 KISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGK 354

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BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 148/364 (40.66%), Postives = 208/364 (57.14%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGS-IGLD 61
           D ++A+  AW+ AC   G   VL P            +G + +  + ++GPCKGS IG  
Sbjct: 22  DISQAVMKAWKAACASQGPSTVLIP------------KGNYNMGEVAMQGPCKGSKIGFQ 81

Query: 62  IKGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWS-SNTSKRPIVTRIA- 121
           I G + AP   +    + W+S Y++D   V G  +L+G G +AW+ +N  K P     A 
Sbjct: 82  IDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAM 141

Query: 122 -------------KITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSGV 181
                         ITS NSK+FH  V  C D+T             NTDGIHV  S GV
Sbjct: 142 NLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGV 201

Query: 182 NIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTF 241
            I ++ I TGDDCIS+GPGS+ + IT ++CGPGHGISIGSLG+Y NE++V  + V+  TF
Sbjct: 202 TITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTF 261

Query: 242 VGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIR 301
            GT NGVR+KTW +S P   T + F +L M +V+NP+I+DQ+YC      + + SR+++ 
Sbjct: 262 SGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLS 321

Query: 302 DVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQP-TSICHNVKGSAFGK 337
           ++ +  IRGTS  + AV   CS   PC  + + +INLS+ G G P TS C NVK +  GK
Sbjct: 322 NINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGK 373

[Close]

BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 142/360 (39.44%), Postives = 201/360 (55.83%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           DS KA+  AW  ACG  G   +L P            +G FLV PL   GPCKG + + +
Sbjct: 55  DSTKAVQEAWASACGGTGKQTILIP------------KGDFLVGPLNFTGPCKGDVTIQV 114

Query: 62  KGELLAPV-AKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWSSNTSKRPIVTRI--- 121
            G LLA      +     W+ I +VDN  + G   L+G G + WS N+  +    +I   
Sbjct: 115 NGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPN 174

Query: 122 ------------AKITSSNSKLFHFAVHGCRDV------------TPNTDGIHVSNSSGV 181
                       + IT  NSK FH  ++ C+D+            +PNTDGIH+ +SSGV
Sbjct: 175 SLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGV 234

Query: 182 NIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTF 241
            I ++VI  GDDCIS+GPG+  +NIT + CGPGHGISIGSLG+Y +E+DV+D+ V+D T 
Sbjct: 235 TITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTL 294

Query: 242 VGTDNGVRIKTWSSSHPSF-VTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQI 301
             T NGVRIK +  +      +KI + N++M+D   P+IID KYC +        S+V +
Sbjct: 295 KKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTV 354

Query: 302 RDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQPT-SICHNVKGSAFG 332
           +DV ++ I GTS+T  AVN  C+   PC G+ + D+N+ ++G    T ++C N KGSA G
Sbjct: 355 KDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKG 402

[Close]

BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-66
Identity = 143/367 (38.96%), Postives = 203/367 (55.31%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           D + A ++AW+EAC    G  +  P            +G ++V  L  +GPCKG + L++
Sbjct: 84  DDSAAFAAAWKEACA--AGSTITVP------------KGEYMVESLEFKGPCKGPVTLEL 143

Query: 62  KGELLAP-VAKNFSIGEYWLSIYQVDNFFVDGPRSL-NGGGSSAWSSNTSKR-------P 121
            G   AP   K       W+    + +F ++G +++ +G GS AW +N   +       P
Sbjct: 144 NGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLP 203

Query: 122 IVTR--------IAKITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSG 181
           I  R        I  ITS+NSKLFH  +  C+++T             NTDGIH+  S+G
Sbjct: 204 INIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNG 263

Query: 182 VNIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDST 241
           VN++ + I TGDDC+S+G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR   
Sbjct: 264 VNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCL 323

Query: 242 FVGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQI 301
              TDNGVRIKTW  S P   + I+F ++ M +V  P++IDQ+YC          S+V++
Sbjct: 324 IKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKL 383

Query: 302 RDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFG 339
            DV  +GI+GTSAT+ AV   CS+  PC  I L DINL  NG  G   S C N+K    G
Sbjct: 384 SDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSG 436

[Close]

BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-66
Identity = 143/364 (39.29%), Postives = 197/364 (54.12%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           D +K    AW+EAC       V+ P            +G +L+  + LEGPCK  I +++
Sbjct: 44  DLSKPFLDAWKEACASVTPSTVVIP------------KGTYLLSKVNLEGPCKAPIEINV 103

Query: 62  KGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWSSNT-------SKRPIV 121
           +G + AP   +      W+  Y V+NF + G    +G GS A+  NT       SK P+ 
Sbjct: 104 QGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVN 163

Query: 122 TR--------IAKITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSGVN 181
            R        I  ITS +SKLFH  V  C+++T            PNTDGIH+  S GVN
Sbjct: 164 IRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVN 223

Query: 182 IMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFV 241
           I+ S I TGDDCIS+G G++ + I  I CGPGHGISIGSLGK+ NEE V  + + + T  
Sbjct: 224 IIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTIT 283

Query: 242 GTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRD 301
            T NG RIKTW   H   V++I F ++ M +V +P++IDQ+YC      K   S+V++ +
Sbjct: 284 NTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSN 343

Query: 302 VRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQL 339
           + ++ IRGTSA   A+ F CS   PC+ + L DI++  NG    TS C NVK    GK  
Sbjct: 344 ISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAEPATSQCLNVKPITIGKLN 395

[Close]

BLAST of CmaCh16G009330 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.5e-67
Identity = 143/367 (38.96%), Postives = 203/367 (55.31%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           D + A ++AW+EAC    G  +  P            +G ++V  L  +GPCKG + L++
Sbjct: 84  DDSAAFAAAWKEACA--AGSTITVP------------KGEYMVESLEFKGPCKGPVTLEL 143

Query: 62  KGELLAP-VAKNFSIGEYWLSIYQVDNFFVDGPRSL-NGGGSSAWSSNTSKR-------P 121
            G   AP   K       W+    + +F ++G +++ +G GS AW +N   +       P
Sbjct: 144 NGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLP 203

Query: 122 IVTR--------IAKITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSG 181
           I  R        I  ITS+NSKLFH  +  C+++T             NTDGIH+  S+G
Sbjct: 204 INIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNG 263

Query: 182 VNIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDST 241
           VN++ + I TGDDC+S+G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR   
Sbjct: 264 VNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCL 323

Query: 242 FVGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQI 301
              TDNGVRIKTW  S P   + I+F ++ M +V  P++IDQ+YC          S+V++
Sbjct: 324 IKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKL 383

Query: 302 RDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFG 339
            DV  +GI+GTSAT+ AV   CS+  PC  I L DINL  NG  G   S C N+K    G
Sbjct: 384 SDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSG 436

[Close]

BLAST of CmaCh16G009330 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 9.5e-67
Identity = 143/367 (38.96%), Postives = 202/367 (55.04%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           D + A ++AW+EAC    G  +  P            +G +LV  L  +GPCKG + L++
Sbjct: 85  DDSAAFAAAWKEACA--AGSTITVP------------KGEYLVESLEFKGPCKGPVTLEL 144

Query: 62  KGELLAP-VAKNFSIGEYWLSIYQVDNFFVDGPRSL-NGGGSSAWSSNTSKR-------P 121
            G   AP   K       W+    + +F ++G +++ +G GS AW +N   +       P
Sbjct: 145 NGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLP 204

Query: 122 IVTR--------IAKITSSNSKLFHFAVHGCRDVT------------PNTDGIHVSNSSG 181
           I  R        I  ITS+NSKLFH  +  C+++T             NTDGIH+  S+G
Sbjct: 205 INIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNG 264

Query: 182 VNIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDST 241
           VN++ + I TGDDC+S+G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR   
Sbjct: 265 VNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCL 324

Query: 242 FVGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQI 301
              TDNGVRIKTW  S P   + I+F ++ M +V  P++IDQ+YC          S+V++
Sbjct: 325 IKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKL 384

Query: 302 RDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFG 339
            DV  + I+GTSAT+ AV   CS+  PC  I L DINL  NG  G   S C N+K    G
Sbjct: 385 SDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSG 437

[Close]

BLAST of CmaCh16G009330 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 8.0e-66
Identity = 139/364 (38.19%), Postives = 196/364 (53.85%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           DS KA   AW+ AC       V  P             G FL+ P+   GPC+  I   I
Sbjct: 43  DSTKAFLGAWQAACRSAAAVTVTVP------------RGSFLLKPVEFRGPCRSRITFQI 102

Query: 62  KGELLAPV-AKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWSSNTSKR--PIVTR-- 121
            G ++AP   +      YW+   +V+   + G  +L+  G+S W+   S +  P+  R  
Sbjct: 103 YGTIVAPSDYRGLGNSGYWILFVKVNRISIIG-GTLDARGASFWACRKSGKSCPVGARSM 162

Query: 122 ---------IAKITSSNSKLFHFAVHGCRDV------------TPNTDGIHVSNSSGVNI 181
                    ++ +TS NS+  H  ++ C +V            +PNTDG+HV  S+GV +
Sbjct: 163 TFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLVAPDQSPNTDGLHVQGSAGVTV 222

Query: 182 MHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVG 241
                 TGDDCIS+GPG+R + ++ ++CGPGHGISIGSLG+  NE  V ++ + +S F G
Sbjct: 223 TDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSLGRDANEAGVENITLINSVFSG 282

Query: 242 TDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDV 301
           +DNGVRIKTW+     FV  ++F NL MK+V+NP+I+DQ YC          S V+I  V
Sbjct: 283 SDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQNYCPSNQGCPKQGSGVKISQV 342

Query: 302 RYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQLP 340
            Y  I+GTS TQ A+ F+CS+  PC+ I L DI L+FN G   TS C N+KG   G  +P
Sbjct: 343 VYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFN-GRSATSTCKNIKGVKAGVVMP 392

[Close]

BLAST of CmaCh16G009330 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.2e-63
Identity = 142/368 (38.59%), Postives = 206/368 (55.98%), Query Frame = 0


Query: 1   MDSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLD 60
           MD+  A ++AW++AC  NG   +  P+            G F +  +   GPC G I   
Sbjct: 46  MDNTNAFTNAWKDACRWNGPSKMYIPL------------GTFYLGGVTFVGPCDGKISFV 105

Query: 61  IKGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAWS-------SNTSKRPI 120
           I G LLAP   +    E W++   ++   V G  +L+G G  +WS       +N  K  I
Sbjct: 106 IDGTLLAPPNNDDIKKEIWINFRYINYLTVFGDGTLDGQGKKSWSLIDCQKDNNCPKLAI 165

Query: 121 --------VTRIAKITSSNSK--------LFHFAVHGCRDV----TPNTDGIHVSNSSGV 180
                    + +  ITS NSK        + HF++     +    +PNTDGI ++ SS +
Sbjct: 166 NMGFDFVKNSSMNGITSLNSKAGHFNFLSVDHFSITRVNIIAPSNSPNTDGIKIALSSNM 225

Query: 181 NIMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTF 240
            I ++ I TGDDCI++  G+   +I N+ CGPGHGISIGSLGK  +E++V+ ++VR+S F
Sbjct: 226 QISNTHISTGDDCIAMLSGNTNFDIYNVKCGPGHGISIGSLGKNKDEKNVNGLMVRNSVF 285

Query: 241 VGTDNGVRIKTWSSSHPSF-VTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQI 300
            GT NG+RIKTW SS  +  +  +V+ NL+M +V NP+ IDQKYC       M  S +QI
Sbjct: 286 TGTTNGIRIKTWESSASTIRIINLVYENLQMINVENPIGIDQKYCPYPPCSNMGDSHIQI 345

Query: 301 RDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFG 340
           R+V  + I GTS  + AV F+CS+ FPC+ + L DINL+ +G  G  +++C NV GSA G
Sbjct: 346 RNVTLKNIWGTSKNKVAVKFQCSKTFPCKDVQLIDINLTHHGVDGPASALCENVDGSATG 401

[Close]

BLAST of CmaCh16G009330 vs. TAIR 10
Match: AT2G15450.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.8e-63
Identity = 143/370 (38.65%), Postives = 202/370 (54.59%), Query Frame = 0


Query: 2   DSAKALSSAWREACGHNGGGVVLEPIVESKYSVEQVGEGRFLVLPLVLEGPCKGSIGLDI 61
           D+A A +S W  AC    G            S   V +G F +  +   GPCK  I   I
Sbjct: 45  DNANAFTSIWSRACKRISGS-----------SKIYVPKGTFYLGGVEFVGPCKNPIEFII 104

Query: 62  KGELLAPVAKNFSIGEYWLSIYQVDNFFVDGPRSLNGGGSSAW-------SSNTSKRPIV 121
            G LLAP   +    + W++   ++N  + G  +L+G G  +W       + N  K  + 
Sbjct: 105 DGTLLAPANPSDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPHNDCHTNPNCPKLAMT 164

Query: 122 --------TRIAKITSSNSKLFH---FAVH--GCRDVT-------PNTDGIHVSNSSGVN 181
                   + I  ITS NSK+ H   F+VH      VT       PNTDGI + + S + 
Sbjct: 165 MGFAFVNNSNIKDITSLNSKMGHFNFFSVHHFNITGVTITAPGDSPNTDGIKMGSCSNIQ 224

Query: 182 IMHSVIDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFV 241
           I  + I TGDDCI++  G+  +NI+N++CGPGHGIS+GSLGK  +E+DV D++VRD  F 
Sbjct: 225 ISDTNIGTGDDCIAILSGTTNLNISNVNCGPGHGISVGSLGKNKDEKDVKDLIVRDVIFN 284

Query: 242 GTDNGVRIKTW-SSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYC--LDCSRDKMSTSRVQ 301
           GT +G+RIK W SS+    V+  V+ N++M DV  P+ IDQKYC    C  ++   S VQ
Sbjct: 285 GTSDGIRIKNWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERKGESHVQ 344

Query: 302 IRDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNG--GGQPTSICHNVKGSA 340
           I++++ + I GTS  + AVN +CS++FPC+ + L DIN+  NG   G  TS+C NV G A
Sbjct: 345 IQNLKLKNIYGTSKNKVAVNLQCSKIFPCKNVELIDINIKQNGVKDGSSTSVCENVDGFA 403

The following BLAST results are available for this feature:

BLAST of CmaCh16G009330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24548	8.6e-73	40.71	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q6H9K0	1.2e-71	40.66	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P35339	2.2e-68	39.44	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
P49063	3.5e-66	38.96	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]
Q39766	3.5e-66	39.29	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]

BLAST of CmaCh16G009330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07850.1	2.5e-67	38.96	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	9.5e-67	38.96	Pectin lyase-like superfamily protein	[more]
AT2G43890.1	8.0e-66	38.19	Pectin lyase-like superfamily protein	[more]
AT1G17150.1	2.2e-63	38.59	Pectin lyase-like superfamily protein	[more]
AT2G15450.1	2.8e-63	38.65	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 267..294 e-value: 4700.0 score: 2.4 coord: 173..193 e-value: 7500.0 score: 1.0 coord: 203..224 e-value: 3.3 score: 16.7 coord: 150..171 e-value: 2100.0 score: 4.9 coord: 122..149 e-value: 1200.0 score: 6.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..337 e-value: 2.2E-77 score: 262.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 36..327 e-value: 5.5E-70 score: 236.0
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 180..193
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..338
None	No IPR available	PANTHER	PTHR31375:SF246	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 2..338
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009330.1	CmaCh16G009330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity