CmaCh14G004650 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G004650
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiondisease resistance protein RGA2-like
LocationCma_Chr14: 2272883 .. 2275327 (-)
RNA-Seq ExpressionCmaCh14G004650
SyntenyCmaCh14G004650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAATTTCATCTACAACCACGCTGAAACTATCTTGAGTAGGCTAAAAAATTGCTCAAACTTCCGAACAATCGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAACTGGCTTAAGGAGCTTCAAAACATCTTTTACCAAATTGAGGACTCCATGGATGAATTCAAATGGGATTTCTTTGAACAAGGGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTTGTGCTCTGATCCAATTTCTGCACATAAATCTAAACTGATTCGTAAAATGAAGAGACTATGCAATCATTTGAATACACTTGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGTAAGACACATGAAACCAAGTTGGCAATTGCAGTACACATTAGTTGATGCTCCAACGGATTATCATGACAATCGGTACGCTGACCTTCTGCAAACTTTCAAATCTAGTCATGGATTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACCATCATAAAGTAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGTCTAAGAAACAATTGCATTTAAAGGTTCGACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGATGGAACTATTGGACATGGGAAACGAGGACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACCGCAGATGCTCTAGGAGTAAGAATAGTCTATGCGTTTGAGAAACAATCGATGCAGAATTCGAACGATAAGAATCAGGAAGTTCCAACAGGCTTCAAACTGGAGAGGCTGTCAGAGGATAAATCAGCTAAGTTATTCAAAAGATATGCTTTCAGAGATAGACAAGAAGTAAACAGAGAAGTATTCACTGAAATAGAAAACCAAATTCTGCAGAAATGCAAGGGAGTTCCATTGGCAATAAAGTGTATGGGAAGCTTGCTATCTTCAAAAATTAGAATAGCTGAGTGGAAGAATATCAAGGACAAGTTGTGGGCACAAGAGGAAGAAAATGACGGTATTTTACATATACTCAGATTATGCTATAATCAAATGCCCTCACACCTGAAGCCCTGTTTTCTTCATTGTGCTCAATTACCAGACCGCATATTATCTTCAAATGACGTGATTCAGTTATGGATGGCACATGGGCTCCTCCATTCACCTGAAGAGAATCACGCATCTATGAAAAATATAGGTGAGAGGTATTTCATGGAGCTATGGTCAAGATGTTTCGTTCAAGAAATTGAAGAACATGGCCTCGGCTACTGGTTTAAACTGCACCCTCTTATCCAAAAACTTGCACACGTACTCGCACAAGAACAATCTGAGGGCTTAGTAGGTACCAAACCAGTCACTCAAATAAAATCCCTAGCCTTCCATGTAAGAGATATGGTGCCACCTAATGCATCCCTACCTGGAATGTGCATTCCGAAGTACAACAGCTTAAGATTGTTACATTTAGGCAATGCAAACATAGAGGAAATTCCAAATGCTATAGGAACACTTACAAGCCTGAGATACCTCGACTTGCATGGAAATAAGAACATCAAGCGGCTGCCGAATTCAATCTGCAATCTACAAAGTCTGCAAACCTTGATTCTTGAATCTTGTTCCGAACTTCAAGAATTGCCAAATGATATAAGGAATTTGATCAGCCTGAAATACTTGTGGATAACAACAAAAGAGCTTCGTTTGCACAAAAACGGAGTTGGAACCATGACTTCTCTGCAATTTCTCGCCATTGGAGGGTGCAAAAACCTACAAATTCTATTCGAACGGCCAGAGTGCCTTGCAGACCTTGAAACCTTAATGATTTACAATTGCGATACTTTGAAATCGTTGCCAAATGAGATGATATATCTAAAATCACTACAGAATTTGGTTATCTGGGGCTGCAAGCAACTAACACTCACCTTAGAGAACGCCGAATTTAGGCTTCAGAGATTCACAATCAGAGAGCTTCCAAAAGTGGAGGAATTACCTGAATGGCTTGACGGATCGATCGGAACTTTGAGAAACCTGCAGATCATCAATTGCCCCATAACCATCAATGTAGGGAGGTTACAAGCGTTTAAAGGTGTTGAAACGGCCAAAATTTTAGGATCTGTAAAAGTCAAAGAGGCGGCGCCGGGTTATGGCTTCCAATATAGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTTCTAA

mRNA sequence

ATGGCTAATTTCATCTACAACCACGCTGAAACTATCTTGAGTAGGCTAAAAAATTGCTCAAACTTCCGAACAATCGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAACTGGCTTAAGGAGCTTCAAAACATCTTTTACCAAATTGAGGACTCCATGGATGAATTCAAATGGGATTTCTTTGAACAAGGGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTTGTGCTCTGATCCAATTTCTGCACATAAATCTAAACTGATTCGTAAAATGAAGAGACTATGCAATCATTTGAATACACTTGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGTAAGACACATGAAACCAAGTTGGCAATTGCAGTACACATTAGTTGATGCTCCAACGGATTATCATGACAATCGGTACGCTGACCTTCTGCAAACTTTCAAATCTAGTCATGGATTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACCATCATAAAGTAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGTCTAAGAAACAATTGCATTTAAAGGTTCGACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGATGGAACTATTGGACATGGGAAACGAGGACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACCGCAGATGCTCTAGGAGTAAGAATAGTCTATGCGTTTGAGAAACAATCGATGCAGAATTCGAACGATAAGAATCAGGAAGTTCCAACAGGCTTCAAACTGGAGAGGCTGTCAGAGGATAAATCAGCTAAGTTATTCAAAAGATATGCTTTCAGAGATAGACAAGAAGTAAACAGAGAAGTATTCACTGAAATAGAAAACCAAATTCTGCAGAAATGCAAGGGAGTTCCATTGGCAATAAAGTGTATGGGAAGCTTGCTATCTTCAAAAATTAGAATAGCTGAGTGGAAGAATATCAAGGACAAGTTGTGGGCACAAGAGGAAGAAAATGACGGTATTTTACATATACTCAGATTATGCTATAATCAAATGCCCTCACACCTGAAGCCCTGTTTTCTTCATTGTGCTCAATTACCAGACCGCATATTATCTTCAAATGACGTGATTCAGTTATGGATGGCACATGGGCTCCTCCATTCACCTGAAGAGAATCACGCATCTATGAAAAATATAGGTGAGAGGTATTTCATGGAGCTATGGTCAAGATGTTTCGTTCAAGAAATTGAAGAACATGGCCTCGGCTACTGGTTTAAACTGCACCCTCTTATCCAAAAACTTGCACACGTACTCGCACAAGAACAATCTGAGGGCTTAGTAGGTACCAAACCAGTCACTCAAATAAAATCCCTAGCCTTCCATGTAAGAGATATGGTGCCACCTAATGCATCCCTACCTGGAATGTGCATTCCGAAGTACAACAGCTTAAGATTGTTACATTTAGGCAATGCAAACATAGAGGAAATTCCAAATGCTATAGGAACACTTACAAGCCTGAGATACCTCGACTTGCATGGAAATAAGAACATCAAGCGGCTGCCGAATTCAATCTGCAATCTACAAAGTCTGCAAACCTTGATTCTTGAATCTTGTTCCGAACTTCAAGAATTGCCAAATGATATAAGGAATTTGATCAGCCTGAAATACTTGTGGATAACAACAAAAGAGCTTCGTTTGCACAAAAACGGAGTTGGAACCATGACTTCTCTGCAATTTCTCGCCATTGGAGGGTGCAAAAACCTACAAATTCTATTCGAACGGCCAGAGTGCCTTGCAGACCTTGAAACCTTAATGATTTACAATTGCGATACTTTGAAATCGTTGCCAAATGAGATGATATATCTAAAATCACTACAGAATTTGGTTATCTGGGGCTGCAAGCAACTAACACTCACCTTAGAGAACGCCGAATTTAGGCTTCAGAGATTCACAATCAGAGAGCTTCCAAAAGTGGAGGAATTACCTGAATGGCTTGACGGATCGATCGGAACTTTGAGAAACCTGCAGATCATCAATTGCCCCATAACCATCAATGTAGGGAGGTTACAAGCGTTTAAAGGTGTTGAAACGGCCAAAATTTTAGGATCTGTAAAAGTCAAAGAGGCGGCGCCGGGTTATGGCTTCCAATATAGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTTCTAA

Coding sequence (CDS)

ATGGCTAATTTCATCTACAACCACGCTGAAACTATCTTGAGTAGGCTAAAAAATTGCTCAAACTTCCGAACAATCGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAACTGGCTTAAGGAGCTTCAAAACATCTTTTACCAAATTGAGGACTCCATGGATGAATTCAAATGGGATTTCTTTGAACAAGGGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTTGTGCTCTGATCCAATTTCTGCACATAAATCTAAACTGATTCGTAAAATGAAGAGACTATGCAATCATTTGAATACACTTGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGTAAGACACATGAAACCAAGTTGGCAATTGCAGTACACATTAGTTGATGCTCCAACGGATTATCATGACAATCGGTACGCTGACCTTCTGCAAACTTTCAAATCTAGTCATGGATTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACCATCATAAAGTAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGTCTAAGAAACAATTGCATTTAAAGGTTCGACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGATGGAACTATTGGACATGGGAAACGAGGACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACCGCAGATGCTCTAGGAGTAAGAATAGTCTATGCGTTTGAGAAACAATCGATGCAGAATTCGAACGATAAGAATCAGGAAGTTCCAACAGGCTTCAAACTGGAGAGGCTGTCAGAGGATAAATCAGCTAAGTTATTCAAAAGATATGCTTTCAGAGATAGACAAGAAGTAAACAGAGAAGTATTCACTGAAATAGAAAACCAAATTCTGCAGAAATGCAAGGGAGTTCCATTGGCAATAAAGTGTATGGGAAGCTTGCTATCTTCAAAAATTAGAATAGCTGAGTGGAAGAATATCAAGGACAAGTTGTGGGCACAAGAGGAAGAAAATGACGGTATTTTACATATACTCAGATTATGCTATAATCAAATGCCCTCACACCTGAAGCCCTGTTTTCTTCATTGTGCTCAATTACCAGACCGCATATTATCTTCAAATGACGTGATTCAGTTATGGATGGCACATGGGCTCCTCCATTCACCTGAAGAGAATCACGCATCTATGAAAAATATAGGTGAGAGGTATTTCATGGAGCTATGGTCAAGATGTTTCGTTCAAGAAATTGAAGAACATGGCCTCGGCTACTGGTTTAAACTGCACCCTCTTATCCAAAAACTTGCACACGTACTCGCACAAGAACAATCTGAGGGCTTAGTAGGTACCAAACCAGTCACTCAAATAAAATCCCTAGCCTTCCATGTAAGAGATATGGTGCCACCTAATGCATCCCTACCTGGAATGTGCATTCCGAAGTACAACAGCTTAAGATTGTTACATTTAGGCAATGCAAACATAGAGGAAATTCCAAATGCTATAGGAACACTTACAAGCCTGAGATACCTCGACTTGCATGGAAATAAGAACATCAAGCGGCTGCCGAATTCAATCTGCAATCTACAAAGTCTGCAAACCTTGATTCTTGAATCTTGTTCCGAACTTCAAGAATTGCCAAATGATATAAGGAATTTGATCAGCCTGAAATACTTGTGGATAACAACAAAAGAGCTTCGTTTGCACAAAAACGGAGTTGGAACCATGACTTCTCTGCAATTTCTCGCCATTGGAGGGTGCAAAAACCTACAAATTCTATTCGAACGGCCAGAGTGCCTTGCAGACCTTGAAACCTTAATGATTTACAATTGCGATACTTTGAAATCGTTGCCAAATGAGATGATATATCTAAAATCACTACAGAATTTGGTTATCTGGGGCTGCAAGCAACTAACACTCACCTTAGAGAACGCCGAATTTAGGCTTCAGAGATTCACAATCAGAGAGCTTCCAAAAGTGGAGGAATTACCTGAATGGCTTGACGGATCGATCGGAACTTTGAGAAACCTGCAGATCATCAATTGCCCCATAACCATCAATGTAGGGAGGTTACAAGCGTTTAAAGGTGTTGAAACGGCCAAAATTTTAGGATCTGTAAAAGTCAAAGAGGCGGCGCCGGGTTATGGCTTCCAATATAGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTTCTAA

Protein sequence

MANFIYNHAETILSRLKNCSNFRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGSGSRKYF
Homology
BLAST of CmaCh14G004650 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 8.1e-69
Identity = 195/683 (28.55%), Postives = 337/683 (49.34%), Query Frame = 0

Query: 22  FRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDF--FEQ 81
           F+ +     +++++L DA+EKQ  ++   L NWL++L    Y+++D +DE+K     F Q
Sbjct: 31  FQRLSSMFSTIQAVLEDAQEKQLNDK--PLENWLQKLNAATYEVDDILDEYKTKATRFLQ 90

Query: 82  GGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKT 141
              G+           +   + K+ ++M ++   LN +A    +FHL EK  +     + 
Sbjct: 91  SEYGR-------YHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQEKIIERQAATRE 150

Query: 142 AHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIG 201
             S L+  +V  R  K   ++   L++  +D      A  L           I+G  G+G
Sbjct: 151 TGSVLTEPQVYGRD-KEKDEIVKILINTASD------AQKLSVLP-------ILGMGGLG 210

Query: 202 KSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLK 261
           K+TL++ +++ + V E++  + W+C+ + F+ +RLI+ +   +  K + +D+    L  K
Sbjct: 211 KTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSL-SDMDLAPLQKK 270

Query: 262 VRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVY 321
           +++LL G  + LV+ D+   +    + L  +L +G   + +L TT+ E+    +G    Y
Sbjct: 271 LQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSIMGTLQPY 330

Query: 322 AFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQ 381
                                +L  LS +    LF + AF  ++E+N  +   I  +I++
Sbjct: 331 ---------------------ELSNLSPEDCWFLFMQRAFGHQEEINPNLMA-IGKEIVK 390

Query: 382 KCKGVPLAIKCMGSLLSSKIRIAEWKNIKDK-LWAQEEENDGILHILRLCYNQMPSHLKP 441
           KC GVPLA K +G +L  K    EW++++D  +W   ++   IL  LRL Y+ +P  L+ 
Sbjct: 391 KCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQ 450

Query: 442 CFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIE 501
           CF++CA  P D  ++  ++I  WMAHG L S  + +  ++++G   + EL+ R F QEIE
Sbjct: 451 CFVYCAVFPKDTKMAKENLIAFWMAHGFLLS--KGNLELEDVGNEVWNELYLRSFFQEIE 510

Query: 502 EHGLGYWFKLHPLIQKLAHVL--AQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGM 561
                 +FK+H LI  LA  L  A   S  +           ++    ++V   +S    
Sbjct: 511 VESGKTYFKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVV---SSYSPS 570

Query: 562 CIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLIL 621
            + K+ SLR+L+L N+N+ ++P++IG L  LRYLDL GN  I+ LP  +C LQ+LQTL L
Sbjct: 571 LLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDL 630

Query: 622 ESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLA---IGGCKNLQILF 681
             C  L  LP     L SL+ L +    L      +G +T L+ L+   IG  K  Q   
Sbjct: 631 HYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQ--- 654

Query: 682 ERPECLADLETLMIYNCDTLKSL 696
                L +L+ L +Y   ++  L
Sbjct: 691 -----LGELKNLNLYGSISITKL 654

BLAST of CmaCh14G004650 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.6e-67
Identity = 193/661 (29.20%), Postives = 335/661 (50.68%), Query Frame = 0

Query: 22  FRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDF--FEQ 81
           F+ +     +++++L DA+EK  Q  N  L NWL++L    Y+++D +DE+K     F Q
Sbjct: 31  FQRLSSMFSTIQAVLEDAQEK--QLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQ 90

Query: 82  GGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKT 141
              G+           +   + K+ ++M ++   L  +A     FHL EK  +     + 
Sbjct: 91  SEYGR-------YHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRE 150

Query: 142 AHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIG 201
             S L+  +V  R  K   ++   L++   +  D ++  +L           I+G  G+G
Sbjct: 151 TGSVLTEPQVYGRD-KEKDEIVKILIN---NVSDAQHLSVLP----------ILGMGGLG 210

Query: 202 KSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLK 261
           K+TLA+ +++ + V E + S+ W+CV E FD +RLI+ +   +  + +  ++    L  K
Sbjct: 211 KTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKK 270

Query: 262 VRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVY 321
           +++LL G  +LLV+ D+   +    + L  +L +G   + +L TT+ E+    +G    Y
Sbjct: 271 LQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSIMGTLQPY 330

Query: 322 AFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQ 381
                                +L  LS++    LF + AF  ++E+N  +   I  +I++
Sbjct: 331 ---------------------ELSNLSQEDCWLLFMQRAFGHQEEINPNL-VAIGKEIVK 390

Query: 382 KCKGVPLAIKCMGSLLSSKIRIAEWKNIKDK-LWAQEEENDGILHILRLCYNQMPSHLKP 441
           K  GVPLA K +G +L  K     W++++D  +W   ++   IL  LRL Y+Q+P  LK 
Sbjct: 391 KSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQ 450

Query: 442 CFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIE 501
           CF +CA  P D  +    +I LWMAHG L S  + +  ++++G+  + EL+ R F QEIE
Sbjct: 451 CFAYCAVFPKDAKMEKEKLISLWMAHGFLLS--KGNMELEDVGDEVWKELYLRSFFQEIE 510

Query: 502 EHGLGYWFKLHPLIQKLAHVL--AQEQSEGL--VGTKPVTQIKSLAFHVRDMVPPNASLP 561
                 +FK+H LI  LA  L  A   S  +  +     T + S+ F     V    +LP
Sbjct: 511 VKDGKTYFKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGF---AEVVFFYTLP 570

Query: 562 GMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTL 621
              + K+ SLR+L+LG++   ++P++IG L  LRYL+L+G+  ++ LP  +C LQ+LQTL
Sbjct: 571 P--LEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTL 630

Query: 622 ILESCSELQELPNDIRNLISLKYLWIT-TKELRLHKNGVGTMTSLQFL---AIGGCKNLQ 671
            L+ C++L  LP +   L SL+ L +  ++ L      +G++T L+ L    +G  K  Q
Sbjct: 631 DLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQ 638

BLAST of CmaCh14G004650 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 9.9e-67
Identity = 199/683 (29.14%), Postives = 332/683 (48.61%), Query Frame = 0

Query: 1   MANFIYNHAETILSRLKNCSNFRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQN 60
           + +FI +    I    K C    ++  T   ++++L DA+EKQ +++   + NWL++L +
Sbjct: 13  LTSFIGDKLVLIFGFEKECEKLSSVFST---IQAVLQDAQEKQLKDK--AIENWLQKLNS 72

Query: 61  IFYQIEDSMDEFKWDF--FEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLA 120
             Y+++D + E K +   FEQ   G            I   + K+ R+MK +   L+ ++
Sbjct: 73  AAYEVDDILGECKNEAIRFEQSRLG-------FYHPGIINFRHKIGRRMKEIMEKLDAIS 132

Query: 121 ARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSV-RHMKPSWQLQYTLVDAPTDYHDNRYA 180
               +FH  EK  +      T  +    TE  V    K   ++   L+      ++   A
Sbjct: 133 EERRKFHFLEKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILI------NNVNVA 192

Query: 181 DLLQTFKSSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEE 240
           + L  F        I+G  G+GK+TLA+ +++ + V + +  + WVCV + FD +RLI+ 
Sbjct: 193 EELPVFP-------IIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKT 252

Query: 241 VY-SVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNE 300
           +  ++        DL+  Q   K+++LL G  +LLV+ D+   +L+  + L  +L +G  
Sbjct: 253 IIGNIERSSPHVEDLASFQ--KKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGAR 312

Query: 301 DSKILVTTQSEETADALGVRIVYAFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKR 360
            + IL TT+ E+    +G    Y                      L  LS   S  LF +
Sbjct: 313 GASILATTRLEKVGSIMGTLQPY---------------------HLSNLSPHDSLLLFMQ 372

Query: 361 YAFRDRQEVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKD-KLWAQE 420
            AF  ++E N  +   I  +I++KC GVPLA K +G LL  K   +EW++++D ++W+  
Sbjct: 373 RAFGQQKEANPNL-VAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLP 432

Query: 421 EENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHA 480
           ++   IL  LRL Y+ +P  L+ CF +CA  P D  +   ++I LWMAHG L S  + + 
Sbjct: 433 QDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLS--KGNL 492

Query: 481 SMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQEQSE----GLVGTK 540
            ++++G   + EL+ R F QEIE      +FK+H LI  LA  L    +       +  K
Sbjct: 493 ELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVK 552

Query: 541 PVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLD 600
                 S+ F         +S     + K+ SLR+L+L  + +E++P++IG L  LRYLD
Sbjct: 553 DYKHTVSIGF-----AAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLD 612

Query: 601 LHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNG 660
           L  N N + LP  +C LQ+LQTL + +C  L  LP     L SL++L +    L      
Sbjct: 613 LSCN-NFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPR 638

Query: 661 VGTMTSLQ---FLAIGGCKNLQI 671
           +G +T L+   F  +G  K  Q+
Sbjct: 673 IGLLTCLKTLGFFIVGSKKGYQL 638

BLAST of CmaCh14G004650 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 1.4e-65
Identity = 185/666 (27.78%), Postives = 328/666 (49.25%), Query Frame = 0

Query: 22  FRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDF--FEQ 81
           F+ +      ++++L DA+EKQ + +   ++NWL++L    Y+++D +D+ K +   F+Q
Sbjct: 31  FKKLSSMFSMIQAVLEDAQEKQLKYK--AIKNWLQKLNVAAYEVDDILDDCKTEAARFKQ 90

Query: 82  GGTGK-KVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMK 141
              G+   +    C         K+ ++MK +   L+ +A     FHL E+  +     +
Sbjct: 91  AVLGRYHPRTITFC--------YKVGKRMKEMMEKLDAIAEERRNFHLDERIIERQAARR 150

Query: 142 TAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGI 201
                L+  +V  R  +    ++  + +               ++        I+G  G+
Sbjct: 151 QTGFVLTEPKVYGREKEEDEIVKILINNV--------------SYSEEVPVLPILGMGGL 210

Query: 202 GKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHL 261
           GK+TLA+ +++ + + E +  + WVCV + FD +RLI+ +   +  K +  D+    L  
Sbjct: 211 GKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSL-GDMDLAPLQK 270

Query: 262 KVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIV 321
           K+++LL G  + LV+ D+   + +    L  +L +G   + IL+TT+ E+    +G   +
Sbjct: 271 KLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQL 330

Query: 322 YAFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQIL 381
           Y                     +L  LS++    LFK+ AF  + E + ++  EI  +I+
Sbjct: 331 Y---------------------QLSNLSQEDCWLLFKQRAFCHQTETSPKLM-EIGKEIV 390

Query: 382 QKCKGVPLAIKCMGSLLSSKIRIAEWKNIKD-KLWAQEEENDGILHILRLCYNQMPSHLK 441
           +KC GVPLA K +G LL  K   +EW++++D ++W   ++ + +L  LRL Y+ +P  L+
Sbjct: 391 KKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLR 450

Query: 442 PCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEI 501
            CF +CA  P D  +    +I LWMAH  L S  + +  ++++G   + EL+ R F QEI
Sbjct: 451 QCFAYCAVFPKDTKIEKEYLIALWMAHSFLLS--KGNMELEDVGNEVWNELYLRSFFQEI 510

Query: 502 EEHGLGYWFKLHPLIQKLAHVL----AQEQSEGLVGTKPVTQIKSLAFHVRDMVPPN--- 561
           E      +FK+H LI  LA  +    A  +S   +  K    +  +  + +DM+      
Sbjct: 511 EVKSGKTYFKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSE 570

Query: 562 --ASLPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNL 621
             +S       ++ SLR+L+L N+  E++P+++G L  LRYLDL GNK I  LP  +C L
Sbjct: 571 VVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNK-ICSLPKRLCKL 630

Query: 622 QSLQTLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQ---FLAIGG 671
           Q+LQTL L +C  L  LP     L SL+ L +    L      +G +T L+   +  +G 
Sbjct: 631 QNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLGYFVVGE 646

BLAST of CmaCh14G004650 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 1.4e-52
Identity = 183/677 (27.03%), Postives = 320/677 (47.27%), Query Frame = 0

Query: 29  MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 88
           +L++ ++L DAEEK  Q  N  +  W+ EL+++ Y  ED++D+   +             
Sbjct: 46  LLTITAVLIDAEEK--QITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN-------- 105

Query: 89  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 148
             + ++  S+++ + +R    L + L+  +  + E  L EK    + R+ +  + L   E
Sbjct: 106 --IGAESSSSNRLRQLRGRMSLGDFLDGNSEHL-ETRL-EKVTIRLERLASQRNILGLKE 165

Query: 149 VSVRHMKPSWQLQYT-LVDAPTDY-HDNRYADLLQTFKSSHG------FFLIVGEAGIGK 208
           ++   M P  +L  T LVD    +  D+   ++++     +G         IVG  G+GK
Sbjct: 166 LTA--MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGK 225

Query: 209 STLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEI-CNDLSKKQLHLK 268
           +TL++ LY+ + V   + ++ W  V E FD  ++ ++VY  V  +     DL   Q+ LK
Sbjct: 226 TTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLK 285

Query: 269 VRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVY 328
            R    G  FLLV+ DL   N  D   L +      + S+ILVTT+S+  A  +    V+
Sbjct: 286 ERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVH 345

Query: 329 AFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQE-VNREVFTEIENQIL 388
                                 L+ LS+     LF +  F +++  +NRE+  ++  +I+
Sbjct: 346 ---------------------NLQPLSDGDCWSLFMKTVFGNQEPCLNREI-GDLAERIV 405

Query: 389 QKCKGVPLAIKCMGSLLSSKIRIAEWKNI-KDKLWAQEEENDGILHILRLCYNQMPSHLK 448
            KC+G+PLA+K +G +L  + ++ EW+ +   ++W    +   +L +LR+ Y  +P+HLK
Sbjct: 406 HKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLK 465

Query: 449 PCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEI 508
            CF +C+  P       + V+ LWMA G L     +  +++ +G  YF EL SR  +Q+ 
Sbjct: 466 RCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSS-KNLEELGNEYFSELESRSLLQKT 525

Query: 509 EEHGLGYWFKLHPLIQKLAHVLAQEQSEGLV-GTK-PVTQIKSLAFHVRD---------- 568
           +       + +H  I +LA   + E S     G K  V++      ++RD          
Sbjct: 526 KTR-----YIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA 585

Query: 569 ------------MVPPNAS--------LPGMCIPKYNSLRLLHLGNANIEEI-PNAIGTL 628
                       +   N+S        +    +P    LR+L L +  I  + P+    +
Sbjct: 586 LREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNI 645

Query: 629 TSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKE 661
           +  R+LDL     +++LP S+C + +LQTL+L  CS L+ELP DI NLI+L+YL +   +
Sbjct: 646 SHARFLDL-SRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTK 677

BLAST of CmaCh14G004650 vs. ExPASy TrEMBL
Match: A0A6J1J5Q0 (putative disease resistance protein RGA1 OS=Cucurbita maxima OX=3661 GN=LOC111481987 PE=4 SV=1)

HSP 1 Score: 1585.5 bits (4104), Expect = 0.0e+00
Identity = 786/786 (100.00%), Postives = 786/786 (100.00%), Query Frame = 0

Query: 29  MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 88
           MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA
Sbjct: 1   MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 60

Query: 89  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 148
           PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE
Sbjct: 61  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 120

Query: 149 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 208
           VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY
Sbjct: 121 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 180

Query: 209 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 268
           HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS
Sbjct: 181 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 240

Query: 269 FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN 328
           FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN
Sbjct: 241 FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN 300

Query: 329 SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI 388
           SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI
Sbjct: 301 SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI 360

Query: 389 KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD 448
           KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD
Sbjct: 361 KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD 420

Query: 449 RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH 508
           RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH
Sbjct: 421 RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH 480

Query: 509 PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL 568
           PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL
Sbjct: 481 PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL 540

Query: 569 GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI 628
           GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI
Sbjct: 541 GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI 600

Query: 629 RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN 688
           RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN
Sbjct: 601 RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN 660

Query: 689 CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS 748
           CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS
Sbjct: 661 CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS 720

Query: 749 IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS 808
           IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS
Sbjct: 721 IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS 780

Query: 809 GSRKYF 815
           GSRKYF
Sbjct: 781 GSRKYF 786

BLAST of CmaCh14G004650 vs. ExPASy TrEMBL
Match: A0A6J1F328 (disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC111441733 PE=4 SV=1)

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 720/794 (90.68%), Postives = 746/794 (93.95%), Query Frame = 0

Query: 25  IEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGK 84
           +EYTMLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD FEQ GTGK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGK 60

Query: 85  KVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFL 144
           KVQAPF CS PISAHK    RKM RL NHL+TLAARMYEFHLTEKHPDSIGR KTAHSFL
Sbjct: 61  KVQAPFSCSHPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRRKTAHSFL 120

Query: 145 STTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLA 204
           STTEVSVRHMKPSWQLQYTL DAP  Y  +RYA+LL TFKS HGFFLIVGEAGIGKSTLA
Sbjct: 121 STTEVSVRHMKPSWQLQYTLTDAPMTYDGDRYAELLTTFKSIHGFFLIVGEAGIGKSTLA 180

Query: 205 KFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLL 264
           KFLY+ K VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+ LL
Sbjct: 181 KFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQHLL 240

Query: 265 KGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYA---- 324
           KGTSFLLVIQDLSTTNLDDSS LMELL MGN++SKILVTTQSEETA ALG+ IVYA    
Sbjct: 241 KGTSFLLVIQDLSTTNLDDSSTLMELLGMGNKNSKILVTTQSEETAGALGLGIVYASEKQ 300

Query: 325 FEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQK 384
            EKQS QNS+DKNQ+V T FKLERLSEDKS+KLFKRYAFRDRQEVN E FT IE+QILQK
Sbjct: 301 SEKQSTQNSDDKNQKVSTVFKLERLSEDKSSKLFKRYAFRDRQEVNNEEFTGIEDQILQK 360

Query: 385 CKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCF 444
           CKGVPLAIKCMGSLLSSK RIAEWKNIKDKLWAQ+EENDGILHILRLCYNQMPSHLKPCF
Sbjct: 361 CKGVPLAIKCMGSLLSSKTRIAEWKNIKDKLWAQKEENDGILHILRLCYNQMPSHLKPCF 420

Query: 445 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHG 504
           LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGE+YFMELWSRCFVQEIEEHG
Sbjct: 421 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGEKYFMELWSRCFVQEIEEHG 480

Query: 505 LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKY 564
           LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVT+IKSLAFHVRDMVPPNASLPGMCI KY
Sbjct: 481 LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTEIKSLAFHVRDMVPPNASLPGMCILKY 540

Query: 565 NSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 624
           NSLR+LHLGNANIEEIPNAIGTL SLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE
Sbjct: 541 NSLRVLHLGNANIEEIPNAIGTLKSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 600

Query: 625 LQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLAD 684
           L+ELP DIRNLISLKYLWITTKEL LHKNGVGTMTSLQFLAIGGCKNL+ILFERPECLAD
Sbjct: 601 LKELPTDIRNLISLKYLWITTKELYLHKNGVGTMTSLQFLAIGGCKNLKILFERPECLAD 660

Query: 685 LETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEE 744
           LETLMIYNC+TLKSLPNEMI+LKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELP V+E
Sbjct: 661 LETLMIYNCNTLKSLPNEMIHLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPGVDE 720

Query: 745 LPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNT 804
           LPEWLDGSI TLRNLQIINCPITINVGRLQAF GVETAKI+GSVKVK AAPGYGFQY NT
Sbjct: 721 LPEWLDGSIETLRNLQIINCPITINVGRLQAFTGVETAKIVGSVKVKGAAPGYGFQYWNT 780

Query: 805 VIRGSSGSGSRKYF 815
           VIRGSSGSGSRKY+
Sbjct: 781 VIRGSSGSGSRKYY 793

BLAST of CmaCh14G004650 vs. ExPASy TrEMBL
Match: A0A0A0LBH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G815400 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 7.9e-277
Identity = 513/890 (57.64%), Postives = 633/890 (71.12%), Query Frame = 0

Query: 1   MANFIYNHAETILSRLKNCSNF-RTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQ 60
           MA+ IY  AE IL++LK+   + + IEY M SLK++L DAEEKQEQ Q   L+NWLKELQ
Sbjct: 1   MAHCIYTQAENILTKLKDSPPYQKRIEYAMSSLKAVLLDAEEKQEQNQR--LQNWLKELQ 60

Query: 61  NIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAA 120
           N+FYQ+EDS+DEFKW+ F+Q   GK+V APF CS+ ISA+K K   K K++C+ LN +A 
Sbjct: 61  NVFYQVEDSIDEFKWEIFKQKDIGKQVLAPFSCSNQISANKLKQKCKRKQVCDELNNIAT 120

Query: 121 RMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADL 180
           RMYEFHL  KH DSI  M+T H+F S +E+S+RH+KPSWQL Y L+DA   Y D  Y  +
Sbjct: 121 RMYEFHLKVKHIDSIS-METTHTFPSASEISIRHLKPSWQLLYPLIDASRTY-DEIYDGI 180

Query: 181 LQTFKSSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVY 240
           L  F      F IVGEAGIGKST+A+FLY+H  VV +Y SR+WVCV+EGF+  RL++EVY
Sbjct: 181 LNVFNECTHVFHIVGEAGIGKSTVARFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVY 240

Query: 241 SVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALM--ELLDMGNED 300
           S    KEIC DL+ +QL  KV++LLK  +FLLV QDLS TNL D S L+  ELL+MG   
Sbjct: 241 SHADNKEICEDLTTEQLLSKVKRLLKVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHG 300

Query: 301 SKILVTTQSEETADALGVRIVYAFEKQSMQN---------------SNDKNQEVP----- 360
           SKI+VTTQ+EE A+ L  R  Y  E++S +N                N +NQ VP     
Sbjct: 301 SKIIVTTQTEEIANYLQDR-GYKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQIE 360

Query: 361 --------------------------------------------------------TGFK 420
                                                                   T FK
Sbjct: 361 ETADAIANKIYEHSLSLTEDAQNLEVPIPQVTEDTNIGLGINPDIPPIKQDDTEYQTIFK 420

Query: 421 LERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRI 480
           LE+LS+  S++LFK YAFR+RQEV     T+I +Q+L+KC GVPLAIKC+GSLLSS+  I
Sbjct: 421 LEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSETSI 480

Query: 481 AEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLW 540
           A+WKNI++KL  QE++ +GILH+LR+CY+QMPS LKPCFLHC+QLP DRI+SSND+IQLW
Sbjct: 481 AKWKNIEEKLSLQEKKENGILHVLRVCYDQMPSQLKPCFLHCSQLPNDRIISSNDMIQLW 540

Query: 541 MAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQ 600
           MA+GLLHSPEE +++M+NIGE+YFMELWSR F+QEIEEHGLGYW KLHPLIQKLAH + Q
Sbjct: 541 MANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEEHGLGYWIKLHPLIQKLAHKITQ 600

Query: 601 EQSEGLVGT---KPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEEIP 660
           EQSEG  G    K VT+I+S+AF  R+MV PNASL   CI KY  LRLL+L NA+++EIP
Sbjct: 601 EQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEKCIWKYKGLRLLYLSNADLQEIP 660

Query: 661 NAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYL 720
           N+IGTL  LRYLDLHGN  IK LPNSICNLQSLQTLIL SCS L++LP DIRNLISL+YL
Sbjct: 661 NSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYL 720

Query: 721 WITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYNCDTLKSLPN 780
           W+TT +LRL KNGVGTM SL+FLAIGGC NL+ LFERP+CLA LETLMIYNC TLK LP+
Sbjct: 721 WVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERPDCLAGLETLMIYNCTTLKLLPD 780

Query: 781 EMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRNLQI 807
           EM YLKSLQNL+IW CKQLTL L+  EF+LQRFTI+ELP+VE LP+WL+ S  TLR LQI
Sbjct: 781 EMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRTLQI 840

BLAST of CmaCh14G004650 vs. ExPASy TrEMBL
Match: A0A6J1DLK5 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111021565 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 1.4e-276
Identity = 511/784 (65.18%), Postives = 607/784 (77.42%), Query Frame = 0

Query: 1   MANFIYNHAETILSRLKNCS-NFRTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQ 60
           MA+FIY+ AE IL++LK+C  + R +EYTMLSLK+IL DAEEK  QEQNH LRNWLKELQ
Sbjct: 41  MAHFIYSRAENILNKLKDCPIDLRRMEYTMLSLKAILLDAEEK--QEQNHGLRNWLKELQ 100

Query: 61  NIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFLCSDPISAH-KSKLIRKMKRLCNHLNTLA 120
           ++FYQ EDS+DE KW   EQ G G+KV+A F CSD  +AH K+    KMK+LC+HL+ +A
Sbjct: 101 DVFYQTEDSIDELKW---EQEGIGRKVRARFSCSDKTAAHKKTNPTSKMKKLCDHLDEIA 160

Query: 121 ARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYAD 180
           ++MYEFHLTE+H  SI RM+TAHSFLS TEVS RHM+PSWQL Y L DAP  Y ++RY +
Sbjct: 161 SKMYEFHLTEEHTSSI-RMETAHSFLSATEVSTRHMRPSWQLFYPLTDAPNSYCNSRYVE 220

Query: 181 LLQTF-KSSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEE 240
           LL  F  S   FF IVGEAGIGKSTLAKF Y H  V   + SR WVCVK+G++T+RL++E
Sbjct: 221 LLNDFIHSKQQFFHIVGEAGIGKSTLAKFFYSHHDVCSSFTSRSWVCVKDGYNTKRLLKE 280

Query: 241 VYSVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNL-DDSSALMELLDMGNE 300
           VYS    KE+ +D +KKQL LKV++ L+G +FL+V QDLS+ NL DD S L  LL++GN 
Sbjct: 281 VYSFQDDKEVRDDFTKKQLLLKVQRFLRGQTFLVVFQDLSSKNLEDDRSMLKNLLELGNY 340

Query: 301 DSKILVTTQSEETADALGVRIVYAFEKQSMQNS---------------NDKNQ----EVP 360
            SKI+VTTQ+EE AD L ++ VY  +K S QN                +++NQ    E  
Sbjct: 341 GSKIIVTTQNEEIADDLKLKKVYKTKKLSEQNQVNTIIHQAINIVEGLSEQNQVNTIEHQ 400

Query: 361 TGFKLERLSEDKSAKLFKRYAFRDRQEV-NREVFTEIENQILQKCKGVPLAIKCMGSLLS 420
               +E LSE  S  LFK YAFRD +E  N E+   I  QI++KCK  PLAIKC+GSLLS
Sbjct: 401 AIIIVEGLSEQNSFSLFKDYAFRDTKEAQNLEL---IAKQIVEKCKRFPLAIKCVGSLLS 460

Query: 421 SKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSND 480
           SK RIAEW  + +KL AQEEE+  IL ILR+CYN MPS+LKPCFL  +QLP DRILSSND
Sbjct: 461 SKNRIAEWNEVNNKLCAQEEEDGSILPILRVCYNLMPSYLKPCFLLYSQLPDDRILSSND 520

Query: 481 VIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLA 540
           VIQLWMAHGLLHSP+EN  S++N GE+YFMELWSRCFVQE+EEHGLG WFKLHPLI+KLA
Sbjct: 521 VIQLWMAHGLLHSPKENSLSLENTGEKYFMELWSRCFVQEMEEHGLGCWFKLHPLIRKLA 580

Query: 541 HVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEE 600
           HVL +EQ+EGLVGTKP T+I+S+AF VRD V P+  L   CI  +  LRLL+LGNA+++E
Sbjct: 581 HVLTREQTEGLVGTKPDTEIRSIAFRVRDKVKPSVFLANKCISMFKCLRLLYLGNADLQE 640

Query: 601 IPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLK 660
           IPNAIGTL  LRYLDL GN+ IK+LPNSIC LQSLQTLIL+SCS L ELPNDI NLISL+
Sbjct: 641 IPNAIGTLKYLRYLDLQGNERIKQLPNSICKLQSLQTLILDSCSSLVELPNDIANLISLR 700

Query: 661 YLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYNCDTLKSL 720
           YLW+T+ +LRLHKNGVGTMTSL+FLAIGGC NLQILFERP+CLA LETLMIYNC+ LKSL
Sbjct: 701 YLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDNLQILFERPDCLAGLETLMIYNCNALKSL 760

Query: 721 PNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRNL 760
           PNEM YLKSLQNLVIWGCKQL LTL+  EF+LQRFTIRELPKVEELP+WL GS  TLR  
Sbjct: 761 PNEMRYLKSLQNLVIWGCKQLKLTLKEVEFKLQRFTIRELPKVEELPQWLQGSSETLR-F 814

BLAST of CmaCh14G004650 vs. ExPASy TrEMBL
Match: A0A1S3B6X8 (putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103486832 PE=4 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 2.6e-272
Identity = 512/893 (57.33%), Postives = 633/893 (70.88%), Query Frame = 0

Query: 1   MANFIYNHAETILSRLKNCSNF-RTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQ 60
           MA  IY  AE IL++LK+C  + + IEYTM SLK+IL DAEEKQEQ Q   L +WLKELQ
Sbjct: 1   MAYCIYTQAENILTKLKDCPAYQKRIEYTMSSLKAILLDAEEKQEQNQR--LHDWLKELQ 60

Query: 61  NIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAA 120
           N+FYQ+EDS+DEFKW+FF+Q  TGK+V A F CS  ISA+K K   K  ++C+ LN +AA
Sbjct: 61  NVFYQVEDSIDEFKWEFFKQKDTGKQVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAA 120

Query: 121 RMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADL 180
            MYEFHL  KH  SI  M+T HSF S +E+S RH+KPSWQL Y L  A  +Y D  Y  +
Sbjct: 121 NMYEFHLKVKHIGSIS-METTHSFPSASEISTRHLKPSWQLLYPLKHASRNY-DEAYDGI 180

Query: 181 LQTF-KSSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEV 240
           L  F K +HGFF IVGEAGIGKST+A+FLY+H+ VV++Y  R+WVCV+EGF+T RL++E+
Sbjct: 181 LNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEI 240

Query: 241 YSVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLD--DSSALMELLDMGNE 300
           YS    KE   DL+ +QL  K  +L +  +FLLV QDLS TNLD   +S L++LL+MG+ 
Sbjct: 241 YSHADNKETREDLTTEQLLSKFIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHH 300

Query: 301 DSKILVTTQSEETADALGVRIVYAFEKQSMQN---------------SNDKNQEVP---- 360
           DSKI+VTTQ+EE A  + V   Y  E QS +N                N +NQ VP    
Sbjct: 301 DSKIIVTTQTEEIAKDIDVH-GYKTEIQSKKNLANGDEVSADKQSLTKNTQNQAVPGSQT 360

Query: 361 -----------------------------------------------------------T 420
                                                                      T
Sbjct: 361 EETAGAIANMIYETEKNLEKNPVAGDGESESSRLITKDAQNLDVSIPQVTECEPDRGYQT 420

Query: 421 GFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSK 480
            FKL++LS+  S+ LFK YAFR+  EV     T+I +Q+L+KC GVPLAIKC+GSLLSSK
Sbjct: 421 IFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSK 480

Query: 481 IRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVI 540
             IAEWKNI+ KL  QE++ DGILH+LR+CY+QMPS LKPCFL+C+QLP DRI SSND+I
Sbjct: 481 TSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMI 540

Query: 541 QLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHV 600
           QLWMA+GLLHSPEE + +M+NIG++YFMELWSRCF+QEIEEHGLGYW KLHPLIQKLAH 
Sbjct: 541 QLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHK 600

Query: 601 LAQEQSEGLVG---TKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIE 660
           + QEQSEG  G   +K +T+I+S+AF  R+MV PNASL   CI +Y  LRLL+LGNA+++
Sbjct: 601 ITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQ 660

Query: 661 EIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISL 720
           EIPN+IGTL  LRYLDLHGN+ IK LP+SIC+LQSLQTLIL+SCS L++LP DIRNLISL
Sbjct: 661 EIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISL 720

Query: 721 KYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYNCDTLKS 780
           +YLW+TT +L LHKNGVGTMTSL+FLAIGGCKNL+ LFERP+CLA LETLMIYNC+TLK 
Sbjct: 721 RYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKL 780

Query: 781 LPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRN 806
           LPNEM YLKSLQNL+IW CKQLTL LE  EF+LQRFT++ELPKVE LP WL+ SI TLR 
Sbjct: 781 LPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRT 840

BLAST of CmaCh14G004650 vs. NCBI nr
Match: XP_022983380.1 (putative disease resistance protein RGA1 [Cucurbita maxima])

HSP 1 Score: 1585.5 bits (4104), Expect = 0.0e+00
Identity = 786/786 (100.00%), Postives = 786/786 (100.00%), Query Frame = 0

Query: 29  MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 88
           MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA
Sbjct: 1   MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 60

Query: 89  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 148
           PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE
Sbjct: 61  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 120

Query: 149 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 208
           VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY
Sbjct: 121 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 180

Query: 209 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 268
           HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS
Sbjct: 181 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 240

Query: 269 FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN 328
           FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN
Sbjct: 241 FLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQN 300

Query: 329 SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI 388
           SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI
Sbjct: 301 SNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQKCKGVPLAI 360

Query: 389 KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD 448
           KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD
Sbjct: 361 KCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLPD 420

Query: 449 RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH 508
           RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH
Sbjct: 421 RILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHGLGYWFKLH 480

Query: 509 PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL 568
           PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL
Sbjct: 481 PLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHL 540

Query: 569 GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI 628
           GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI
Sbjct: 541 GNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDI 600

Query: 629 RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN 688
           RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN
Sbjct: 601 RNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLADLETLMIYN 660

Query: 689 CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS 748
           CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS
Sbjct: 661 CDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGS 720

Query: 749 IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS 808
           IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS
Sbjct: 721 IGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGS 780

Query: 809 GSRKYF 815
           GSRKYF
Sbjct: 781 GSRKYF 786

BLAST of CmaCh14G004650 vs. NCBI nr
Match: XP_022934599.1 (disease resistance protein RGA2-like [Cucurbita moschata])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 720/794 (90.68%), Postives = 746/794 (93.95%), Query Frame = 0

Query: 25  IEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGK 84
           +EYTMLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD FEQ GTGK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGK 60

Query: 85  KVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFL 144
           KVQAPF CS PISAHK    RKM RL NHL+TLAARMYEFHLTEKHPDSIGR KTAHSFL
Sbjct: 61  KVQAPFSCSHPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRRKTAHSFL 120

Query: 145 STTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLA 204
           STTEVSVRHMKPSWQLQYTL DAP  Y  +RYA+LL TFKS HGFFLIVGEAGIGKSTLA
Sbjct: 121 STTEVSVRHMKPSWQLQYTLTDAPMTYDGDRYAELLTTFKSIHGFFLIVGEAGIGKSTLA 180

Query: 205 KFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLL 264
           KFLY+ K VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+ LL
Sbjct: 181 KFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQHLL 240

Query: 265 KGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYA---- 324
           KGTSFLLVIQDLSTTNLDDSS LMELL MGN++SKILVTTQSEETA ALG+ IVYA    
Sbjct: 241 KGTSFLLVIQDLSTTNLDDSSTLMELLGMGNKNSKILVTTQSEETAGALGLGIVYASEKQ 300

Query: 325 FEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQK 384
            EKQS QNS+DKNQ+V T FKLERLSEDKS+KLFKRYAFRDRQEVN E FT IE+QILQK
Sbjct: 301 SEKQSTQNSDDKNQKVSTVFKLERLSEDKSSKLFKRYAFRDRQEVNNEEFTGIEDQILQK 360

Query: 385 CKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCF 444
           CKGVPLAIKCMGSLLSSK RIAEWKNIKDKLWAQ+EENDGILHILRLCYNQMPSHLKPCF
Sbjct: 361 CKGVPLAIKCMGSLLSSKTRIAEWKNIKDKLWAQKEENDGILHILRLCYNQMPSHLKPCF 420

Query: 445 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHG 504
           LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGE+YFMELWSRCFVQEIEEHG
Sbjct: 421 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGEKYFMELWSRCFVQEIEEHG 480

Query: 505 LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKY 564
           LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVT+IKSLAFHVRDMVPPNASLPGMCI KY
Sbjct: 481 LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTEIKSLAFHVRDMVPPNASLPGMCILKY 540

Query: 565 NSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 624
           NSLR+LHLGNANIEEIPNAIGTL SLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE
Sbjct: 541 NSLRVLHLGNANIEEIPNAIGTLKSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 600

Query: 625 LQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLAD 684
           L+ELP DIRNLISLKYLWITTKEL LHKNGVGTMTSLQFLAIGGCKNL+ILFERPECLAD
Sbjct: 601 LKELPTDIRNLISLKYLWITTKELYLHKNGVGTMTSLQFLAIGGCKNLKILFERPECLAD 660

Query: 685 LETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEE 744
           LETLMIYNC+TLKSLPNEMI+LKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELP V+E
Sbjct: 661 LETLMIYNCNTLKSLPNEMIHLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPGVDE 720

Query: 745 LPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNT 804
           LPEWLDGSI TLRNLQIINCPITINVGRLQAF GVETAKI+GSVKVK AAPGYGFQY NT
Sbjct: 721 LPEWLDGSIETLRNLQIINCPITINVGRLQAFTGVETAKIVGSVKVKGAAPGYGFQYWNT 780

Query: 805 VIRGSSGSGSRKYF 815
           VIRGSSGSGSRKY+
Sbjct: 781 VIRGSSGSGSRKYY 793

BLAST of CmaCh14G004650 vs. NCBI nr
Match: XP_023528962.1 (putative disease resistance protein RGA4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 705/794 (88.79%), Postives = 740/794 (93.20%), Query Frame = 0

Query: 25  IEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGK 84
           +EYTMLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD  EQ GTGK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-SEQEGTGK 60

Query: 85  KVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFL 144
           KVQAPF CSDPISAHK    RKM RL NHL+TLAARMYEFHLTEKHPDSIGRMKTAHSFL
Sbjct: 61  KVQAPFSCSDPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFL 120

Query: 145 STTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLA 204
           STTEVSVRHMKPSWQLQYTL DAP  Y  + YA+LL TFKS HGFFLIVGEAGIGKSTLA
Sbjct: 121 STTEVSVRHMKPSWQLQYTLTDAPMAYDGDPYAELLTTFKSIHGFFLIVGEAGIGKSTLA 180

Query: 205 KFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLL 264
           KFLYHH+ VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+QLL
Sbjct: 181 KFLYHHQEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLL 240

Query: 265 KGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYA---- 324
           KGTSFLLVIQDLSTTNLDDS+ LM+LL MGNE+SKILVTTQSE TA ALG+R VYA    
Sbjct: 241 KGTSFLLVIQDLSTTNLDDSNTLMKLLGMGNENSKILVTTQSEMTAGALGLRTVYASEKQ 300

Query: 325 FEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFTEIENQILQK 384
            EKQS QNS+DKNQ+V TGFKLERLSEDKS+KLFKRYAFRDRQEVN E FT IE+QILQK
Sbjct: 301 SEKQSTQNSDDKNQKVSTGFKLERLSEDKSSKLFKRYAFRDRQEVNNEEFTGIEDQILQK 360

Query: 385 CKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQMPSHLKPCF 444
           CKGVPLAIKCMGSLLSSK RIAEWKNIKDKLWAQ+EENDGILHILRLCYNQMPSHLKPCF
Sbjct: 361 CKGVPLAIKCMGSLLSSKTRIAEWKNIKDKLWAQKEENDGILHILRLCYNQMPSHLKPCF 420

Query: 445 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEIEEHG 504
           LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGE+YFMELWSRCFVQEIEEHG
Sbjct: 421 LHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGEKYFMELWSRCFVQEIEEHG 480

Query: 505 LGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNASLPGMCIPKY 564
           LGYWFKLHPLIQKLAHVLAQE+SEGLVGTKPVT+IKSLAFHVRDMVPPNA LPGMCI KY
Sbjct: 481 LGYWFKLHPLIQKLAHVLAQERSEGLVGTKPVTEIKSLAFHVRDMVPPNAFLPGMCILKY 540

Query: 565 NSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 624
            SLR+LHLGNANIEEIPNAIGTLT+LRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE
Sbjct: 541 KSLRVLHLGNANIEEIPNAIGTLTNLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSE 600

Query: 625 LQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQILFERPECLAD 684
           L+ELP DIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCK+LQILFERPECL D
Sbjct: 601 LKELPTDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKDLQILFERPECLVD 660

Query: 685 LETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFTIRELPKVEE 744
           LETLMIYNCD L SLPNEMIYLKSLQNLVIWGCKQLTLTLENA+FRLQRFTIRELPKV++
Sbjct: 661 LETLMIYNCDNLMSLPNEMIYLKSLQNLVIWGCKQLTLTLENAKFRLQRFTIRELPKVKK 720

Query: 745 LPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAPGYGFQYRNT 804
           LPEWLDGS+ TLRNLQIINCP+T+NV RLQAF+GVETAKILGSVKV+E  PGYGFQY+NT
Sbjct: 721 LPEWLDGSVQTLRNLQIINCPVTMNVERLQAFEGVETAKILGSVKVRETPPGYGFQYKNT 780

Query: 805 VIRGSSGSGSRKYF 815
           VIR   G  S+K +
Sbjct: 781 VIRVPRGIDSKKEY 793

BLAST of CmaCh14G004650 vs. NCBI nr
Match: KAG6580847.1 (Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 644/803 (80.20%), Postives = 670/803 (83.44%), Query Frame = 0

Query: 25  IEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGK 84
           +EYTMLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD FEQ GTGK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGK 60

Query: 85  KVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFL 144
           KVQAPF CS PISAHK K  RKM RL NHL+TLAARMYEFHLTEKHPDSIGRMKTAHSFL
Sbjct: 61  KVQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFL 120

Query: 145 STTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLA 204
           STTEVS RHMKPSWQLQYTL DAP  Y  NRYA+LL+TFKS  GFFLIVGEAGIGKSTLA
Sbjct: 121 STTEVSARHMKPSWQLQYTLTDAPMAYDGNRYAELLRTFKSIRGFFLIVGEAGIGKSTLA 180

Query: 205 KFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLL 264
           KFLY+ K VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+QLL
Sbjct: 181 KFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLL 240

Query: 265 KGTSFLLVIQDLSTTNLDDSSALMELLDMGN---------EDSKILVTTQSEETADALGV 324
           KGTSFLLVIQDLSTTNLDDSSAL+ELL MGN         ++SKILVTTQSEETA ALG+
Sbjct: 241 KGTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKILVTTQNSKILVTTQSEETAGALGL 300

Query: 325 RIVYA----FEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREVFT 384
            IVYA     EKQS QNS+DKNQ+V T FKLERLSEDKS+KLFKRYAFRDRQEV  E FT
Sbjct: 301 GIVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLSEDKSSKLFKRYAFRDRQEVTNEEFT 360

Query: 385 EIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHILRLCYNQ 444
            IE+QILQKCKGVPLAIKCMGSLLSSK RIAEWKNIKDKLWAQ+EEND            
Sbjct: 361 GIEDQILQKCKGVPLAIKCMGSLLSSKTRIAEWKNIKDKLWAQKEEND------------ 420

Query: 445 MPSHLKPCFLHCAQLPDRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRC 504
                                                                       
Sbjct: 421 ------------------------------------------------------------ 480

Query: 505 FVQEIEEHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTQIKSLAFHVRDMVPPNAS 564
                 EHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVT+IKSLAFHVRDMVPPNAS
Sbjct: 481 ------EHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGTKPVTEIKSLAFHVRDMVPPNAS 540

Query: 565 LPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQ 624
           LPGMCI KYNSLR+LHLGNANIEEIPN IGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQ
Sbjct: 541 LPGMCILKYNSLRVLHLGNANIEEIPNDIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQ 600

Query: 625 TLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLAIGGCKNLQIL 684
           TLILESCSEL+ELP DIRNLISLKYLWITTKEL LHKNGVGTMTSLQFLAIGGCKNL+IL
Sbjct: 601 TLILESCSELKELPTDIRNLISLKYLWITTKELYLHKNGVGTMTSLQFLAIGGCKNLKIL 660

Query: 685 FERPECLADLETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTLENAEFRLQRFT 744
           FERPECLADLETLMIYNC+TLKSLPNEMI+LKSLQNLVIWGCKQLTLTLENAEFRLQRFT
Sbjct: 661 FERPECLADLETLMIYNCNTLKSLPNEMIHLKSLQNLVIWGCKQLTLTLENAEFRLQRFT 720

Query: 745 IRELPKVEELPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKILGSVKVKEAAP 804
           IRELP V+ELPEWLDGSI TLRNLQIINCPITINVGRLQAF GVETAKI+GSVKVK AAP
Sbjct: 721 IRELPGVDELPEWLDGSIETLRNLQIINCPITINVGRLQAFTGVETAKIVGSVKVKGAAP 724

Query: 805 GYGFQYRNTVIRGSSGSGSRKYF 815
           GYGFQY NTVIRGSSGSGSRKY+
Sbjct: 781 GYGFQYWNTVIRGSSGSGSRKYY 724

BLAST of CmaCh14G004650 vs. NCBI nr
Match: XP_038904479.1 (disease resistance protein RGA2-like [Benincasa hispida])

HSP 1 Score: 1038.5 bits (2684), Expect = 3.1e-299
Identity = 551/874 (63.04%), Postives = 658/874 (75.29%), Query Frame = 0

Query: 1   MANFIYNHAETILSRLKNCSNFRT-IEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQ 60
           MA+FIY  AE IL++LK+C  ++  IEYTMLSLK+IL DAE+K  QEQN  L +WLKELQ
Sbjct: 1   MAHFIYLQAENILTKLKDCPTYQNRIEYTMLSLKAILLDAEKK--QEQNQYLHDWLKELQ 60

Query: 61  NIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAA 120
           N+FYQIEDS+DEFKW+FF+Q GT K+V A F CS  ISA+K +  RKM++LC+ LN + A
Sbjct: 61  NVFYQIEDSIDEFKWEFFKQEGTRKQVCAHFSCSAQISANKLRQTRKMEKLCDQLNAITA 120

Query: 121 RMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSVRHMKPSWQLQYTLVDAPTD-----YHDN 180
           RMYEFHL EKH  S+  M+T H FLS TE+S+RHMKPSWQL Y L DAP D      ++ 
Sbjct: 121 RMYEFHLKEKHIASLS-METTHPFLSATEISIRHMKPSWQLLYPLTDAPRDDDFAEQYNQ 180

Query: 181 RYADLLQTFK-SSHGFFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQR 240
           RY DLL  F  S+H FF IVGEAG+GKSTLAKFLY+HK V+E+Y SR+WVCV+EGFDTQR
Sbjct: 181 RYKDLLSDFNGSTHRFFHIVGEAGMGKSTLAKFLYNHKSVIEKYTSRYWVCVEEGFDTQR 240

Query: 241 LIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDM 300
           L++EVY+    KEIC +L+ KQL  KVR+ LK  +FLL+ QDLS TNL D   L  +L+M
Sbjct: 241 LMKEVYNHADNKEICEELTMKQLLSKVRRSLKERTFLLIFQDLSITNLGDCDILKNILEM 300

Query: 301 GNEDSKILVTTQSEETADALGVRIVYAF---EKQSMQN--SND-------------KNQE 360
           GN DSKI+VTTQSEE A AL +  VY +   EKQS Q   +ND             +NQ 
Sbjct: 301 GNHDSKIIVTTQSEEIAKALKLERVYRWPMTEKQSKQKLVANDGESVGNSSIAVDAQNQA 360

Query: 361 VP-------------------------------TGFKLERLSEDKSAKLFKRYAFRDRQE 420
           VP                               T + LERLSE+ S  LFK YAF+D QE
Sbjct: 361 VPGPHDTGPIRFSYDLRFHPSIPQNEQDVMTYQTIYTLERLSEENSILLFKHYAFKDGQE 420

Query: 421 VNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGILHI 480
             +   TEI  QI+QKCKGVPLAIKC+G+LLSSK RIAEW+N+++KL + +E++D IL I
Sbjct: 421 AEKTEITEIAKQIVQKCKGVPLAIKCLGNLLSSKTRIAEWQNVENKLRSLQEKDDSILPI 480

Query: 481 LRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERY 540
           LRLCYNQMPS LKPCFL C+QLP DRILSSND+IQLWMAHGLLHSPEE + SM+NIGE+Y
Sbjct: 481 LRLCYNQMPSQLKPCFLLCSQLPNDRILSSNDMIQLWMAHGLLHSPEEINLSMENIGEKY 540

Query: 541 FMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGT---KPVTQIKSLAF 600
           FMELWSRCF+QEIEEHGLGYW K+HPLI KLAHVL QEQSEG  GT   KP T+I+S+AF
Sbjct: 541 FMELWSRCFIQEIEEHGLGYWVKVHPLIIKLAHVLTQEQSEGSEGTKPPKPFTEIRSIAF 600

Query: 601 HVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLHGNKNIKRL 660
            VR+MV PNASLP  CI K+  LRLL+LGNAN++EIPN+IGTL +LRYLDLHGN+ IK+L
Sbjct: 601 QVRNMVLPNASLPEKCISKFKCLRLLYLGNANLQEIPNSIGTLKNLRYLDLHGNQKIKQL 660

Query: 661 PNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFL 720
           PN+IC+L SLQTLILESCS L+ LP DIRNLISL+YLW+TT +L LHKNGVGTM+SL+FL
Sbjct: 661 PNAICDLLSLQTLILESCSSLEGLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMSSLRFL 720

Query: 721 AIGGCKNLQILFERPECLADLETLMIYNCDTLKSLPNEMIYLKSLQNLVIWGCKQLTLTL 780
           AIGGC NLQ LFE+P+CLA LETLMIY+C+TLK LPNEM +LKSLQNL+IW CKQLTLTL
Sbjct: 721 AIGGCHNLQNLFEQPDCLAGLETLMIYDCNTLKLLPNEMRFLKSLQNLMIWSCKQLTLTL 780

Query: 781 ENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRNLQIINCPITINVGRLQAFKGVETAKI 815
           E  EFRLQRFTI+ELPKVEELP WL+GS  TLR+LQIINCPI +  G   +   VE   I
Sbjct: 781 EAVEFRLQRFTIKELPKVEELPRWLEGSAETLRSLQIINCPIRMG-GMFVSLNAVERIII 840

BLAST of CmaCh14G004650 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 209.5 bits (532), Expect = 9.9e-54
Identity = 183/677 (27.03%), Postives = 320/677 (47.27%), Query Frame = 0

Query: 29  MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 88
           +L++ ++L DAEEK  Q  N  +  W+ EL+++ Y  ED++D+   +             
Sbjct: 46  LLTITAVLIDAEEK--QITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN-------- 105

Query: 89  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 148
             + ++  S+++ + +R    L + L+  +  + E  L EK    + R+ +  + L   E
Sbjct: 106 --IGAESSSSNRLRQLRGRMSLGDFLDGNSEHL-ETRL-EKVTIRLERLASQRNILGLKE 165

Query: 149 VSVRHMKPSWQLQYT-LVDAPTDY-HDNRYADLLQTFKSSHG------FFLIVGEAGIGK 208
           ++   M P  +L  T LVD    +  D+   ++++     +G         IVG  G+GK
Sbjct: 166 LTA--MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGK 225

Query: 209 STLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEI-CNDLSKKQLHLK 268
           +TL++ LY+ + V   + ++ W  V E FD  ++ ++VY  V  +     DL   Q+ LK
Sbjct: 226 TTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLK 285

Query: 269 VRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVY 328
            R    G  FLLV+ DL   N  D   L +      + S+ILVTT+S+  A  +    V+
Sbjct: 286 ERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVH 345

Query: 329 AFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQE-VNREVFTEIENQIL 388
                                 L+ LS+     LF +  F +++  +NRE+  ++  +I+
Sbjct: 346 ---------------------NLQPLSDGDCWSLFMKTVFGNQEPCLNREI-GDLAERIV 405

Query: 389 QKCKGVPLAIKCMGSLLSSKIRIAEWKNI-KDKLWAQEEENDGILHILRLCYNQMPSHLK 448
            KC+G+PLA+K +G +L  + ++ EW+ +   ++W    +   +L +LR+ Y  +P+HLK
Sbjct: 406 HKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLK 465

Query: 449 PCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEI 508
            CF +C+  P       + V+ LWMA G L     +  +++ +G  YF EL SR  +Q+ 
Sbjct: 466 RCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSS-KNLEELGNEYFSELESRSLLQKT 525

Query: 509 EEHGLGYWFKLHPLIQKLAHVLAQEQSEGLV-GTK-PVTQIKSLAFHVRD---------- 568
           +       + +H  I +LA   + E S     G K  V++      ++RD          
Sbjct: 526 KTR-----YIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA 585

Query: 569 ------------MVPPNAS--------LPGMCIPKYNSLRLLHLGNANIEEI-PNAIGTL 628
                       +   N+S        +    +P    LR+L L +  I  + P+    +
Sbjct: 586 LREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNI 645

Query: 629 TSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKE 661
           +  R+LDL     +++LP S+C + +LQTL+L  CS L+ELP DI NLI+L+YL +   +
Sbjct: 646 SHARFLDL-SRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTK 677

BLAST of CmaCh14G004650 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 166.8 bits (421), Expect = 7.4e-41
Identity = 166/692 (23.99%), Postives = 309/692 (44.65%), Query Frame = 0

Query: 23  RTIEYTMLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFF----- 82
           + ++  +++   +L DA+++ E  +  ++++WL  +++ F+Q ED +DE + +       
Sbjct: 37  KRLKVALVTANPVLADADQRAEHVR--EVKHWLTGIKDAFFQAEDILDELQTEALRRRVV 96

Query: 83  -EQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGR 142
            E GG G   Q      + I   + K+  KM+++         R+ E H+  KH + IG 
Sbjct: 97  AEAGGLGGLFQNLMAGREAI---QKKIEPKMEKV--------VRLLEHHV--KHIEVIGL 156

Query: 143 MKTAHSFLSTTEVSVRHMKPSWQ--LQYTLVDAPTDYHDNRYAD-------LLQTFKSSH 202
            + + +            +P W+   +    D P      R  D       LL   + S 
Sbjct: 157 KEYSET-----------REPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISI 216

Query: 203 G---FFLIVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVAC 262
           G      +VG  G+GK+TL + +++   V E +  + W+     F+   + + V   +  
Sbjct: 217 GKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITS 276

Query: 263 KEICNDLSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTT 322
             + N      L +++++ L G  FLLV+ D  + +  +  +         E SKI++TT
Sbjct: 277 SAV-NTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTT 336

Query: 323 QSEETADALGVRIVYAFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQ- 382
           +SE  +       +Y                     +++ ++ ++  +L  R+AF +   
Sbjct: 337 RSEIVSTVAKAEKIY---------------------QMKLMTNEECWELISRFAFGNISV 396

Query: 383 -EVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEEENDGIL 442
             +N+E+   I  +I ++CKG+PLA + + S L SK    +W  +           + IL
Sbjct: 397 GSINQEL-EGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNF---SSYTNSIL 456

Query: 443 HILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGE 502
            +L+L Y+ +P  LK CF  C+  P   +    +++ LWMA  LL+ P  +   +++IG 
Sbjct: 457 PVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSR-RLEDIGN 516

Query: 503 RYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQE-----QSEGLVGTKPVTQ-- 562
            Y  +L ++ F Q ++       F +H L+  LA  ++ +     + + +      T+  
Sbjct: 517 DYLGDLVAQSFFQRLDITMTS--FVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 576

Query: 563 -------IKSLAFH-------VRDMVPPNA--SLPGMCIPK---------YNSLRLLHLG 622
                    S+AF        +R ++P N+  SL  + + +          + LR+L L 
Sbjct: 577 SFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLS 636

Query: 623 NANIEEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIR 662
           +  I  +P ++  L  LRYLDL   K IK LP  +C L +LQTL+L +C +L  LP  I 
Sbjct: 637 HYQITNLPKSLKGLKLLRYLDLSSTK-IKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIA 672

BLAST of CmaCh14G004650 vs. TAIR 10
Match: AT1G59780.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 148.7 bits (374), Expect = 2.1e-35
Identity = 173/766 (22.58%), Postives = 331/766 (43.21%), Query Frame = 0

Query: 32  LKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQAPFL 91
           L + L DA+ K++       RN L+E++ I Y  ED ++     F  +G    +  A F 
Sbjct: 46  LMAFLSDADAKKQTRAL--ARNCLEEIKEITYDAEDIIE----IFLLKGSVNMRSLACFP 105

Query: 92  CS-DPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVS 151
                I+   + + +++ ++   +  L  +       + H     + +  H+F S +E +
Sbjct: 106 GGRREIALQITSISKRISKVIQVMQNLGIKSDIMDGVDSHAQLERKRELRHTFSSESESN 165

Query: 152 VRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLYHH 211
           +  ++ + +               +  + L    SSHG   I G  G+GK+TLA+ ++ H
Sbjct: 166 LVGLEKNVE---------------KLVEELVGNDSSHG-VSITGLGGLGKTTLARQIFDH 225

Query: 212 KVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTSFL 271
             V   +    WVCV + F  + + + +   ++ K   +DL +  +  K+ QLL+    L
Sbjct: 226 DKVKSHFDGLAWVCVSQEFTRKDVWKTILGNLSPKYKDSDLPEDDIQKKLFQLLETKKAL 285

Query: 272 LVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETADALGVRIVYAFEKQSMQNSN 331
           +V  DL     +D   +  +        K+L+T++                        N
Sbjct: 286 IVFDDL--WKREDWYRIAPMFPERKAGWKVLLTSR------------------------N 345

Query: 332 DKNQEVPTGFKLERLSEDKSAKLFKRYAFRDRQEVNREV----FTEIENQILQKCKGVPL 391
           D        FK E L+ D+  KL +R AF  ++ +   +      ++  ++ + CK +PL
Sbjct: 346 DAIHPHCVTFKPELLTHDECWKLLQRIAFSKQKTITGYIIDKEMVKMAKEMTKHCKRLPL 405

Query: 392 AIKCMGSLLSSKIRIAEWKNIKDKL---------WAQEEENDGILHILRLCYNQMPSHLK 451
           A+K +G LL +K  + +WK I + +          + E ++  + H+L L +  +P +LK
Sbjct: 406 AVKLLGGLLDAKHTLRQWKLISENIISHIVVGGTSSNENDSSSVNHVLSLSFEGLPGYLK 465

Query: 452 PCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMKNIGERYFMELWSRCFVQEI 511
            C L+ A  P D  +    +  +W A G+ +      A+++++ + Y  EL  R  V   
Sbjct: 466 HCLLYLASYPEDHEIEIERLSYVWAAEGITYPGNYEGATIRDVADLYIEELVKRNMVIS- 525

Query: 512 EEHGLGYWF---KLHPLIQKLAHVLAQEQSEGLVGTKPV--TQIKSLA-FHVRDMVPPNA 571
           E   L   F   +LH L++++  + A+E++   + T P   + + SLA    R +V  N 
Sbjct: 526 ERDALTSRFEKCQLHDLMREICLLKAKEENFLQIVTDPTSSSSVHSLASSRSRRLVVYNT 585

Query: 572 SL--------------------------PGMCIPKYNSLRLLHLGNANIE--EIPNAIGT 631
           S+                           G    +   LR+L L  A  +  ++P++IG 
Sbjct: 586 SIFSGENDMKNSKLRSLLFIPVGYSRFSMGSNFIELPLLRVLDLDGAKFKGGKLPSSIGK 645

Query: 632 LTSLRYLDLHGNKNIKRLPNSICNLQSLQTLILE-SCSELQELPNDIRNLISLKYLWITT 691
           L  L+YL L+   ++  LP+S+ NL+SL  L L  +  +L  +PN  + ++ L+YL +  
Sbjct: 646 LIHLKYLSLY-QASVTYLPSSLRNLKSLLYLNLRINSGQLINVPNVFKEMLELRYLSLPW 705

Query: 692 KELRLHKNGVGTMTSLQFLAIGGCKNLQIL-FERPECLADLETLMIYNCDTLKSLPNEMI 746
           +   L K  +G +  L+ L     K+  +    R   L  L+ L+      +++L + + 
Sbjct: 706 ERSSLTKLELGNLLKLETLINFSTKDSSVTDLHRMTKLRTLQILISGEGLHMETLSSALS 755

BLAST of CmaCh14G004650 vs. TAIR 10
Match: AT1G59620.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 145.2 bits (365), Expect = 2.3e-34
Identity = 149/549 (27.14%), Postives = 247/549 (44.99%), Query Frame = 0

Query: 192 IVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL 251
           I G  GIGK+TLA+ +++H+ V   +    WVCV + F  + + + +   V  + I  ++
Sbjct: 155 ITGMGGIGKTTLARQVFNHETVKSHFAQLAWVCVSQQFTRKYVWQTILRKVGPEYIKLEM 214

Query: 252 SKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMGNEDSKILVTTQSEETAD 311
           ++ +L  K+ +LL     L+V+ D+     +D   +  +  +G +  K+L+T+++E    
Sbjct: 215 TEDELQEKLFRLLGTRKALIVLDDI--WREEDWDMIEPIFPLG-KGWKVLLTSRNE---- 274

Query: 312 ALGVRIVYAFEKQSMQNSNDKNQEVPTG--FKLERLSEDKSAKLFKRYAFRDRQEVNREV 371
             GV +                +  P G  FK + L+ ++S  +F+R  F        +V
Sbjct: 275 --GVAL----------------RANPNGFIFKPDCLTPEESWTIFRRIVFPGENTTEYKV 334

Query: 372 ---FTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWK----NIKDKLWAQEEEND--- 431
                E+  Q+++ C G+PLA+K +G LL     + EWK    NIK  +      ND   
Sbjct: 335 DEKMEELGKQMIKHCGGLPLALKVLGGLLVVHFTLDEWKRIYGNIKSHIVGGTSFNDKNM 394

Query: 432 -GILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHASMK 491
             + HIL L + ++P +LK CFL+ AQ P D  +    +   W A G+      + A+++
Sbjct: 395 SSVYHILHLSFEELPIYLKHCFLYLAQFPEDFTIDLEKLSYYWAAEGMPRPRYYDGATIR 454

Query: 492 NIGERYFMELWSRCFVQEIEEHGLGYWFK---LHPLIQKLAHVLAQEQSEGLVGT---KP 551
            +G+ Y  EL  R  V   E       F+   LH +++++  + A+E  E L+ T   K 
Sbjct: 455 KVGDGYIEELVKRNMVIS-ERDARTRRFETCHLHDIVREVCLLKAEE--ENLIETENSKS 514

Query: 552 VTQIKSLAFHVRDMVPPNASLPGMCIPKYNSL----------------------RLLHLG 611
            ++ + L     D       L     PK  SL                      R+L L 
Sbjct: 515 PSKPRRLVVKGGDKTDMEGKLKN---PKLRSLLFIEELGGYRGFEVWFTRLQLMRVLDLH 574

Query: 612 NANI-EEIPNAIGTLTSLRYLDLHGNKNIKRLPNSICNLQSLQTL---ILESCSELQELP 671
                 E+P++IG L  LRYL L+  K    LP+S+ NL+ L  L   + ESC     +P
Sbjct: 575 GVEFGGELPSSIGLLIHLRYLSLYRAK-ASHLPSSMQNLKMLLYLNLCVQESC--YIYIP 634

Query: 672 NDIRNLISLKYLWITTKELRLHKNGVGTMTSLQFLA--------IGGCKNLQILFERPEC 687
           N ++ ++ LKYL   +  LR+    +G    LQF+         I G  N++ L      
Sbjct: 635 NFLKEMLELKYL---SLPLRMDDKSMGEWGDLQFMTRLRALSIYIRGRLNMKTLSSSLSK 666

BLAST of CmaCh14G004650 vs. TAIR 10
Match: AT1G58390.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 145.2 bits (365), Expect = 2.3e-34
Identity = 164/704 (23.30%), Postives = 307/704 (43.61%), Query Frame = 0

Query: 17  KNCSNFRTIEYTMLSLK-------SILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSM 76
           + C  F+ +E  +  LK       S L DA+ K  +     +RN ++E++ I Y  ED +
Sbjct: 19  QECEQFQGVEDQVTGLKRDLNLLSSFLKDADAK--KHTTAVVRNVVEEIKEIVYDAEDII 78

Query: 77  DEF--KWDFFEQGGTGKKVQAPFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLT 136
           + +  K   ++  G   +++             + +I   +R    +  +  R+ +    
Sbjct: 79  ETYLLKEKLWKTSGIKMRIR-----------RHACIISDRRRNALDVGGIRTRISDV--- 138

Query: 137 EKHPDSIGRMKTAHSF-LSTTEVSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSS 196
                    ++   SF +    V   +M+P    Q  +    +  +++ +  L    K  
Sbjct: 139 ---------IRDMQSFGVQQAIVDGGYMQPQGDRQREMRQTFSKDYESDFVGLEVNVKKL 198

Query: 197 HGFFL---------IVGEAGIGKSTLAKFLYHHKVVVEQYLSRFWVCVKEGFDTQRLIEE 256
            G+ +         I G  G+GK+TLA+ +++H+ V  Q+    WVCV + F  + + + 
Sbjct: 199 VGYLVDEENVQVVSITGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKNVWQM 258

Query: 257 VYSVVACKEICND---LSKKQLHLKVRQLLKGTSFLLVIQDLSTTNLDDSSALMELLDMG 316
           +   +  +E  ++   + + +LH K+ QLL+ +  L+V  D+     D+   L++ +   
Sbjct: 259 ILQNLTSREKKDEILQMEEAELHDKLFQLLETSKSLIVFDDIWK---DEDWDLIKPIFPP 318

Query: 317 NEDSKILVTTQSEETADALGVRIVYAFEKQSMQNSNDKNQEVPTGFKLERLSEDKSAKLF 376
           N+  K+L+T+Q+E  A    ++ +                     FK E L+ + S  LF
Sbjct: 319 NKGWKVLLTSQNESVAVRGDIKYL--------------------NFKPECLAIEDSWTLF 378

Query: 377 KRYAFRDRQEVNREVFTEIEN---QILQKCKGVPLAIKCMGSLLSSKIRIAEWKNI---- 436
           +R AF  +     +V  E+E+   Q+L+ C G+PLAIK +G LL++K  + +W+ +    
Sbjct: 379 QRIAFPKKDASESKVDEEMEDMGKQMLKHCGGLPLAIKVLGGLLAAKYTMHDWERLSVNI 438

Query: 437 -KDKLWAQEEENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGL 496
             D +      N  I H+L + + ++PS+LK CFL+ A  P D  ++   +   W A G+
Sbjct: 439 GSDIVGRTSSNNSSIYHVLSMSFEELPSYLKHCFLYLAHFPEDHKINVEKLSYCWAAEGI 498

Query: 497 LHSPE-ENHASMKNIGERYFMELWSRCFV---QEIEEHGLGYWFKLHPLIQKLAHVLAQE 556
             + +  N  +++++G+ Y  EL  R  +   ++      G    LH +++++    A+E
Sbjct: 499 STAEDYHNGETIQDVGQSYLEELVRRNMIIWERDATASRFG-TCHLHDMMREVCLFKAKE 558

Query: 557 Q-------------------------SEGLVGTKPVT----------QIKSLAFHVRDMV 616
           +                         S  LV   P T          +++SL     D+ 
Sbjct: 559 ENFLQIAVKSVGVTSSSTGNSQSPCRSRRLVYQCPTTLHVERDINNPKLRSLVVLWHDLW 618

Query: 617 PPNASLPGMCIPKYNSLRLLHLGNANIE--EIPNAIGTLTSLRYLDLHGNKNIKRLPNSI 649
             N  L G    +   LR+L L   + E  ++P  IG L  LRYL L   K +  LP+S+
Sbjct: 619 VENWKLLGTSFTRLKLLRVLDLFYVDFEGMKLPFGIGNLIHLRYLSLQDAK-VSHLPSSL 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XA428.1e-6928.55Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XBQ92.6e-6729.20Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA399.9e-6729.14Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA401.4e-6527.78Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR41.4e-5227.03Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1J5Q00.0e+00100.00putative disease resistance protein RGA1 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1F3280.0e+0090.68disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC1114417... [more]
A0A0A0LBH97.9e-27757.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G815400 PE=4 SV=1[more]
A0A6J1DLK51.4e-27665.18putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A1S3B6X82.6e-27257.33putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103486832... [more]
Match NameE-valueIdentityDescription
XP_022983380.10.0e+00100.00putative disease resistance protein RGA1 [Cucurbita maxima][more]
XP_022934599.10.0e+0090.68disease resistance protein RGA2-like [Cucurbita moschata][more]
XP_023528962.10.0e+0088.79putative disease resistance protein RGA4 [Cucurbita pepo subsp. pepo][more]
KAG6580847.10.0e+0080.20Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038904479.13.1e-29963.04disease resistance protein RGA2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G14470.19.9e-5427.03NB-ARC domain-containing disease resistance protein [more]
AT3G14460.17.4e-4123.99LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G59780.12.1e-3522.58NB-ARC domain-containing disease resistance protein [more]
AT1G59620.12.3e-3427.14Disease resistance protein (CC-NBS-LRR class) family [more]
AT1G58390.12.3e-3423.30Disease resistance protein (CC-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 605..621
score: 36.32
coord: 189..204
score: 47.03
coord: 263..277
score: 27.67
coord: 381..395
score: 41.83
NoneNo IPR availableGENE3D1.20.5.4130coord: 12..121
e-value: 1.7E-10
score: 42.9
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 547..765
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 21..74
e-value: 6.0E-10
score: 39.3
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 344..428
e-value: 6.9E-15
score: 56.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 429..517
e-value: 3.0E-9
score: 38.8
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 562..618
e-value: 1.1E-6
score: 28.2
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 585..607
score: 7.080498
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 185..430
e-value: 4.4E-27
score: 94.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 171..317
e-value: 2.2E-20
score: 74.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 176..443
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 549..772
e-value: 5.1E-35
score: 122.5
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 28..761

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G004650.1CmaCh14G004650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding
molecular_function GO:0005515 protein binding