CmaCh14G001200 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G001200
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCma_Chr14: 519524 .. 520782 (-)
RNA-Seq ExpressionCmaCh14G001200
SyntenyCmaCh14G001200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCTTCAATATCCATTTCATTCTTCAATTCGCTGCAGATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCTCTCGTCACCGGCGGAACCCGAGGCATTGGGTCGCCCTCCAATCATTATCAATCCCACTTCTCATGTCATTCATCTATTTATGTTCTTACTTCTTTTGTTTTATTTTGTTTTCAGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTACCCAAACAGAGCTGGACCAGAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCACCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTAATTCTATCTCTCATTTATTTTCTTTACAAACCCAAAATGCTCAAATTCTGAAAATGGGTGGTTGGTTTTAAGGTGAACAACGCAGGGACGATAACACTGAAATCAGCAGCAGAGCACACCACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGTATGGGGTTAGATAAATCTGTGGGTAATGAATGATTGGAAGATTCAAGATTGTTCTTTGACGGTTTGTGGGTTTGTACTTCGATTGCAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACACCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTAGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACATGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTTAGTATGGGCTGTGCATTTGTGTGGTGTAGTGTAACAATGTTTCAGTTTATGTGTATCATCGAAAGTGGCAAAACGTTAAACAGTAGGCACAATGTCTCAGTCTGAAAGAAAATAAAATGTATTAATCGGCCATGTGATGTGATAAGGTCCTCCTTTGGGCATTCATTTTCTATTTTAAGTTTCGG

mRNA sequence

ATCTCTTCAATATCCATTTCATTCTTCAATTCGCTGCAGATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCTCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTACCCAAACAGAGCTGGACCAGAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCACCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGACGATAACACTGAAATCAGCAGCAGAGCACACCACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACACCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTAGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACATGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTTAGTATGGGCTGTGCATTTGTGTGGTGTAGTGTAACAATGTTTCAGTTTATGTGTATCATCGAAAGTGGCAAAACGTTAAACAGTAGGCACAATGTCTCAGTCTGAAAGAAAATAAAATGTATTAATCGGCCATGTGATGTGATAAGGTCCTCCTTTGGGCATTCATTTTCTATTTTAAGTTTCGG

Coding sequence (CDS)

ATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCTCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTACCCAAACAGAGCTGGACCAGAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCACCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGACGATAACACTGAAATCAGCAGCAGAGCACACCACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACACCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTAGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACATGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAG

Protein sequence

MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTGQVICVDGGYTAGGG
Homology
BLAST of CmaCh14G001200 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.4e-85
Identity = 160/256 (62.50%), Postives = 197/256 (76.95%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIGYAIVEELA+ GA V+TCSR+Q +LD+ +++W RKGF+VSG
Sbjct: 11  RRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVSG 70

Query: 66  SVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAP 125
            VCD+SS S R+ L+ +V+S FNGKLNIL+NNAGT   K A   T EDY+ IM TNFEA 
Sbjct: 71  PVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYSIIMGTNFEAS 130

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           Y+L Q+++P LKASG  SIVF SS AGV+A+P  SIYAASKGAINQ+TK+LACEWAKD+I
Sbjct: 131 YNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAKDSI 190

Query: 186 RINTVAPWAVKTTISKPDAAVVEE---YSRLLERTPAGRIGEANEISSVVAFLCLPAASY 245
           R+N VAPW + T I +    V  +      L+ R P  R GE +E+SS+V +LCLP ASY
Sbjct: 191 RVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPTASY 250

Query: 246 MTGQVICVDGGYTAGG 259
           +TGQ+ICVDGGYT  G
Sbjct: 251 ITGQIICVDGGYTVNG 266

BLAST of CmaCh14G001200 vs. ExPASy Swiss-Prot
Match: P50162 (Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.4e-83
Identity = 156/257 (60.70%), Postives = 197/257 (76.65%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIGYAIVEELA LGA V+TCSR + ELD+ ++ W  KG  V GS
Sbjct: 16  RWSLKGTTALVTGGSKGIGYAIVEELAGLGARVYTCSRNEKELDECLEIWREKGLNVEGS 75

Query: 67  VCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCDL SR+ R++L+ TV+ +F+GKLNILVNNAG +  K A + T +DYN IM TNFEA Y
Sbjct: 76  VCDLLSRTERDKLMQTVAHVFDGKLNILVNNAGVVIHKEAKDFTEKDYNIIMGTNFEAAY 135

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI+YP LKAS  G+++F+SS+AG  ALP VS+Y+ASKGAINQ+TK+LACEWAKDNIR
Sbjct: 136 HLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASKGAINQMTKSLACEWAKDNIR 195

Query: 187 INTVAPWAVKT----TISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASY 246
           +N+VAP  + T    T  K +    EE    + +TP GR G+  E+S+++AFLC PAASY
Sbjct: 196 VNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIAFLCFPAASY 255

Query: 247 MTGQVICVDGGYTAGGG 260
           +TGQ+I  DGG+TA GG
Sbjct: 256 ITGQIIWADGGFTANGG 272

BLAST of CmaCh14G001200 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.4e-83
Identity = 149/253 (58.89%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R +T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R  RE+L+ TVSS+F GKLNILVNN GT+ LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTG 245
           R N + PW + T +     +V E      ERTP GR+GEANE+S +VAFLCLPAASY+TG
Sbjct: 183 RTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITG 242

Query: 246 QVICVDGGYTAGG 259
           QVICVDGG T  G
Sbjct: 243 QVICVDGGLTVNG 255

BLAST of CmaCh14G001200 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 3.1e-83
Identity = 153/252 (60.71%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R +T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR  RE+L+ TVSS+F GKLNILVNNAGT  +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASKGA+NQ+ ++LACEWA DNIR
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASKGAMNQLGRSLACEWASDNIR 192

Query: 187 INTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTGQ 246
           +N+V PW + T ++    +  +    + ++TP GR+GEANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG +  G
Sbjct: 253 TICVDGGASVNG 263

BLAST of CmaCh14G001200 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 7.0e-83
Identity = 151/249 (60.64%), Postives = 195/249 (78.31%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R +T+L + V+EW+ KGFEV+ S
Sbjct: 13  RWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVREWQAKGFEVTTS 72

Query: 67  VCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR  RE+L+  V+SIF GKLNILVNNAGT   K   E+T +DY+ +M+TN ++ +
Sbjct: 73  VCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDYSFLMATNLDSAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ+++P LKASG GSIV +SS AGVV +   SIY A+KGA+NQ+ KNLACEWA+DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLMSSTAGVVHINVGSIYGATKGAMNQLAKNLACEWARDNIR 192

Query: 187 INTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTGQ 246
           +N+V PW + T +   D  + +E  R   +TP GR+G ANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWFIATPLYLNDEELKKEVER---KTPMGRVGNANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYT 256
            ICVDGG+T
Sbjct: 253 TICVDGGFT 258

BLAST of CmaCh14G001200 vs. ExPASy TrEMBL
Match: A0A6J1J921 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.4e-139
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SYMTGQVICVDGGYTAGGG
Sbjct: 241 SYMTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. ExPASy TrEMBL
Match: A0A6J1F1Z8 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 6.3e-135
Identity = 249/259 (96.14%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS R+QLITTVSSIFNGKLNILVNNAG +TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTIS+PDAAVVEEYSRLLE TPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. ExPASy TrEMBL
Match: A0A5D3DN35 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001440 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.7e-120
Identity = 216/258 (83.72%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSR QTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S S R+QLI TVSSIFNG LNILVNNAGT+TLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPK+SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+NTVAPW V+TTISKPDAAVVEEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGG 259
           SY++GQ+ICVDGG+TAGG
Sbjct: 241 SYVSGQIICVDGGFTAGG 258

BLAST of CmaCh14G001200 vs. ExPASy TrEMBL
Match: A0A1S3B6I2 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.7e-120
Identity = 216/258 (83.72%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSR QTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S S R+QLI TVSSIFNG LNILVNNAGT+TLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPK+SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+NTVAPW V+TTISKPDAAVVEEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGG 259
           SY++GQ+ICVDGG+TAGG
Sbjct: 241 SYVSGQIICVDGGFTAGG 258

BLAST of CmaCh14G001200 vs. ExPASy TrEMBL
Match: A0A0A0LAG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.8e-119
Identity = 214/258 (82.95%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGA+VHTCSR QTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S S R+QLI TVSSIFNG LNILVNNAGT+T+K+A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTMKTATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALP++SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPW V+TTIS PDAA VEEY RL+ RTPAGR+GE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWGVRTTISTPDAAAVEEYGRLIGRTPAGRLGEPEEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGG 259
           SY++GQ+ICVDGGYTAGG
Sbjct: 241 SYVSGQIICVDGGYTAGG 258

BLAST of CmaCh14G001200 vs. NCBI nr
Match: XP_022983913.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 505.4 bits (1300), Expect = 3.0e-139
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SYMTGQVICVDGGYTAGGG
Sbjct: 241 SYMTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. NCBI nr
Match: XP_023527559.1 (tropinone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.1 bits (1276), Expect = 1.8e-136
Identity = 253/259 (97.68%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS REQLITTVSSIFNGKLNILVNNAGT+TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. NCBI nr
Match: KAG7017273.1 (hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492.7 bits (1267), Expect = 2.0e-135
Identity = 250/259 (96.53%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 6   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 65

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS R+QLITTVSSIFNGKLNILVNNAG +TLKSAAEHTTEDYNNIMST
Sbjct: 66  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 125

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 126 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 185

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTIS+PDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 186 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 245

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SY+TGQVICVDGGYTAGGG
Sbjct: 246 SYVTGQVICVDGGYTAGGG 264

BLAST of CmaCh14G001200 vs. NCBI nr
Match: KAG6580523.1 (hypothetical protein SDJN03_20525, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 492.7 bits (1267), Expect = 2.0e-135
Identity = 250/259 (96.53%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS R+QLITTVSSIFNGKLNILVNNAG +TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTIS+PDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. NCBI nr
Match: XP_022934187.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 490.0 bits (1260), Expect = 1.3e-134
Identity = 249/259 (96.14%), Postives = 255/259 (98.46%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS R+QLITTVSSIFNGKLNILVNNAG +TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRINTVAPWAVKTTIS+PDAAVVEEYSRLLE TPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYMTGQVICVDGGYTAGGG 260
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of CmaCh14G001200 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.0e-84
Identity = 149/253 (58.89%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R +T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R  RE+L+ TVSS+F GKLNILVNN GT+ LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTG 245
           R N + PW + T +     +V E      ERTP GR+GEANE+S +VAFLCLPAASY+TG
Sbjct: 183 RTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITG 242

Query: 246 QVICVDGGYTAGG 259
           QVICVDGG T  G
Sbjct: 243 QVICVDGGLTVNG 255

BLAST of CmaCh14G001200 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 2.2e-84
Identity = 153/252 (60.71%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R +T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR  RE+L+ TVSS+F GKLNILVNNAGT  +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASKGA+NQ+ ++LACEWA DNIR
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASKGAMNQLGRSLACEWASDNIR 192

Query: 187 INTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTGQ 246
           +N+V PW + T ++    +  +    + ++TP GR+GEANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG +  G
Sbjct: 253 TICVDGGASVNG 263

BLAST of CmaCh14G001200 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 3.2e-83
Identity = 148/252 (58.73%), Postives = 194/252 (76.98%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIG A+VEELA+LGA +HTC+R +T+L + +++W+ KGF+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 67  VCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR  RE+L+ TVS+IF GKLNILVNN GT  +K   +HT ED++  M+TN E+ +
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ+++P LKASG GSIV +SSV+GVV +   SIY  SKGA+NQ+ +NLACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 187 INTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTGQ 246
            N+V PW ++T +     +  E    +  R P GR+GE NE+SS+VAFLCLPAASY+TGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG+T  G
Sbjct: 253 TICVDGGFTVNG 264

BLAST of CmaCh14G001200 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 3.2e-83
Identity = 155/253 (61.26%), Postives = 190/253 (75.10%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIG A+VEELA  GA VHTCSR Q EL+  + +W+  G  VSG
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 66  SVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD S R  RE+LI   SS F+GKLNIL+NN GT   K   E+++E+Y  IMSTN E+ 
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +HLSQI++P LKASG GSIVF+SSVAG+V L   SIY A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 186 RINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTG 245
           R N VAPW +KT++ +      E    ++ RTP GR+GE  E+SS+VAFLCLPA+SY+TG
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITG 244

Query: 246 QVICVDGGYTAGG 259
           QVI VDGG+T  G
Sbjct: 245 QVISVDGGFTVNG 257

BLAST of CmaCh14G001200 vs. TAIR 10
Match: AT2G29340.2 (NAD-dependent epimerase/dehydratase family protein )

HSP 1 Score: 304.3 bits (778), Expect = 9.4e-83
Identity = 152/253 (60.08%), Postives = 189/253 (74.70%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGG  GIGYAIVEELA  GA +H C  ++ +L+Q + EWE+KGF+VSG
Sbjct: 3   KRWSLKGMTALVTGGASGIGYAIVEELAGFGARIHVCDISEAKLNQSLSEWEKKGFQVSG 62

Query: 66  SVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD++SR  RE+L+ TVSS F+GKLNILV+N G I  K   E+T +D+   +S+N EA 
Sbjct: 63  SVCDVASRPEREELMQTVSSQFDGKLNILVSNVGVIRSKPTTEYTEDDFAFHISSNVEAA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           YH SQ+S+P LKASGYGSI+FVSS+AGV++    SIY  +KGA+ Q+ KNLACEWAKD I
Sbjct: 123 YHFSQLSHPLLKASGYGSIIFVSSIAGVISFDAGSIYGLTKGALIQLAKNLACEWAKDGI 182

Query: 186 RINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYMTG 245
           R N VAP  + T +S+     V     LL RTP GR+GE NE++S+VAFLCLPAASY+TG
Sbjct: 183 RANAVAPNVINTPLSQSYLEDVSFKKALLSRTPLGRVGEPNEVASLVAFLCLPAASYITG 242

Query: 246 QVICVDGGYTAGG 259
           Q ICVDGG T  G
Sbjct: 243 QTICVDGGLTVNG 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P501653.4e-8562.50Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1[more]
P501621.4e-8360.70Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1[more]
Q9ZW131.4e-8358.89Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Q9ZW033.1e-8360.71Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Q9ZW047.0e-8360.64Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Match NameE-valueIdentityDescription
A0A6J1J9211.4e-139100.00tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=... [more]
A0A6J1F1Z86.3e-13596.14tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 S... [more]
A0A5D3DN359.7e-12083.72Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B6I29.7e-12083.72tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1[more]
A0A0A0LAG74.8e-11982.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022983913.13.0e-139100.00tropinone reductase homolog [Cucurbita maxima][more]
XP_023527559.11.8e-13697.68tropinone reductase homolog [Cucurbita pepo subsp. pepo][more]
KAG7017273.12.0e-13596.53hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6580523.12.0e-13596.53hypothetical protein SDJN03_20525, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022934187.11.3e-13496.14tropinone reductase homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G29290.21.0e-8458.89NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29150.12.2e-8460.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.13.2e-8358.73NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G06060.13.2e-8361.26NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29340.29.4e-8360.08NAD-dependent epimerase/dehydratase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 14..31
score: 50.59
coord: 136..152
score: 35.68
coord: 218..238
score: 32.66
coord: 89..100
score: 58.08
coord: 183..200
score: 34.07
coord: 162..181
score: 41.85
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 89..100
score: 51.01
coord: 162..181
score: 38.33
coord: 142..150
score: 49.39
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 21..255
e-value: 5.0E-56
score: 189.9
NoneNo IPR availableGENE3D3.40.50.720coord: 2..259
e-value: 1.0E-79
score: 269.4
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 10..255
e-value: 2.1E-17
score: 61.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..200
e-value: 3.7E-20
score: 70.1
NoneNo IPR availablePANTHERPTHR42898:SF28TROPINONE REDUCTASE-LIKE PROTEINcoord: 5..258
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 5..258
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 149..177
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G001200.1CmaCh14G001200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity