CmaCh12G006700 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G006700
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNADH-quinone oxidoreductase subunit N
LocationCma_Chr12: 3604690 .. 3607740 (+)
RNA-Seq ExpressionCmaCh12G006700
SyntenyCmaCh12G006700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCTATGGGTCTACTGGAGCTACCCACTTCGATCAATTAGCCAATATTTTGACCGGATACGAAATCACTGGTGCTCGATCTAGTGGTATTTTTATGGGGATTCTATCTATCGCTGTAGGATTCCTATTCAAGATCACTGCAGTTCCTTTTCGGGCGGCTGTAGGACGGACGGCCGCCTATAGGTGGTAGGGTAGGGTGGGTGGTACCGCTCAGATTGTGGCCAATCTTCCTAACCGCGCGCGGGCCGAGCTTAGAGCGCGTGAAACTCATCACTACCTCGTAAGGGCGTTGAGACCATAGCATGTTACACGAAAGCGCCGCTTTCTCTGTAGTCTTGTCACACAGCAGCCCGCCTAGAAGAGCCACTCGCTCTGTAGTGTTGTCACATAAGATAAGCACTAGCCCGCCTGATGGCCGGGCGAATCTAAGTTCTCTTCCGTTCAACTGTCCACCCAGTCAAGTGCAAAAAACACAGTAGAATCACGCAAGGCATCGAGCTGGTGTGCTTCCTGCCCGCGAGGAAAGAAAGAAGAGCGACAGGGTTGAGTTCAGATTTGACTGTTTGCAGCATGGGAGCAGATTACCCAAAAAATCCCTGGGAACAATGAAAAAAAAGATATCTCGGTAACGAAAACTATGGGGGGCTGTATTGGCGAGATCCAACGGTGGACAGCTGCCCAAAAGAAAAGCCGCCTGGAAGTCCGAGGACCTTTAGTACCCTACCCCCAAACCACCTTCGCGCCAAGCAAGACCGCCCTTGTCCCTTTCCTTTCTCCATTCCGCCTCCCCTTCTTTCTGAGCTTTGTTCCAATCGAGTCTAAGGCAAAGCAAAAGTCGTTCGTATACCTCTTTTTTGACCTACTTGACGAAAGGGAACAAAGTTTCGTTTCCGGGGTTTATGGATTGGATTCAGTCAGCTAAACTCATTCCTTTTTATTATGTTGTGACGGGTTACCGGCGAACGCTTCCAAAGGCGACCCTCTCGAGTTTCCGGCGGTTTACGTCATTGAAGTAGTCTTTCGTCATCCTACCAAACTAAAGAAGTCACTATCAAGCAGCGAGCCCTTATTAGTTTAGTAAAGTCAAGCTAAAAAAACGAGAGATTAACCTGCGGTGCGCTACGCCCGGCAACTCATGAAGTAGGGCTCCTTCCTATTGACTAAAGGTGGATTCTTCGCTTTCCTTGAGAATGAAAGAAAGTAGCTATTCAGCCCCTACTACTGACTTTTGACTTTGTTTGATTCAAAAGGCGAACAGCCAGCCCCCTCAACTAGTCGTATGAGGTGGGGTGCTTGTGGTAAGCCGCTTTGGATATGAGGAATTCCTAAAAAAAAGAAAAGGCAAAGTCCGGTATTTGACGAAGATCTTTCGATCAATAGATAGGATTTAATGTTCAATATAGGGGATAGGATCCATTCTTTCTCAATTTGATTTAGAGAGAGAGATATAACGATATCAAAACAAAGAAATAGGGAGATGATAAAGAATACATAGACAGAAAAAGGGATGTCTATTCTATTCCTCTTTGTTTCTTTTTCAGTGCAAGACAGGAAACCTCCTTTTGTCATCCCTGCAGCTTTCTTTTGTATTGAACGCATGGCCTAGGACCTGAAAATTATCGCCTATGTTCACACCCCTCTTTCTTTGAATCAGGCTAGTAAAGAATGCCCGCCAAGTTCAGATAAGGAAATGCTTCCCCCAACCATTAAAGGCTCGACGAAGGGAGGGGAAGGAAAATGCTTTCGGAGATCGATATTGGATTTCTTTTGAATCAAAAACGAAAAAGGCCGAGGATGGCCTACAGTGCGTCTTATCTGAAGGGAACACGCCTTTTCGAAAGCGGTGGTATGATTGCCGGGCCCTCTCCTCGTTCTGCCCGCTGGCCTATTAGGTAGGATAGCAGTCTTCGGGCTTTGCCTGCCCTTTCTCATCAAGAATTTCGGCTTGGCCCGGGAAAGCGCTGGCAACAAAAGGAAGGGGTCCATGTAGCTGCTGCGCCCGCCCCCTTCTTAGTCAATGGGGCAGCAGGTTCGGCATCCACTAGAAAAGAGAGAATCCACTTAGATAACCATGCCTCTGCTAGGCGGCAGCGTGTGGAATAGCCGAGAGCAGACCTTTTTGGATATATAATCCAAGTCGAGAGTGGAGTTACGGAAAGAGCCGTCTGATGGAAAACAACTTTCACGTTCGGTTCAGAGAGCACTGTTTTCGTTGATCATTCCTCGTTCCCTTTCGTGTGAATTCCACAGCGGCGAATTCTCAACTTGTGGGGCCCTATCTATTCCATCTCTCAAGCCCCAAAGAAAAACCCCCTACCCTTCGGACTCCATATCTCTTTTGACTCTATATATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACACCATTCCTTTCTATTGCGCCTAAAATCTCTATTTTTGCTAATATTTCACGTCTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGTAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACTGGTTTCTCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTATCTTGATTTATGTATTAATGACAATAGATGCATTCGCTATAGTTTTAGCATTACGGCAACCTCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGTTGTTACCTTCTCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCGTTGGGCGGCCGGAAGGTTGCCACGAGTAAGTCAGTCCGTTTGGGAGACCGAAGACAGTTCTCCGTGCACCGGACACGTAGCTTACCGAATCCGTTGCGACACGGATGGGAATGCATGCTTCGAAAGATAG

mRNA sequence

ATGATCTATGGGTCTACTGGAGCTACCCACTTCGATCAATTAGCCAATATTTTGACCGGATACGAAATCACTGGTGCTCGATCTAGTGGTATTTTTATGGGGATTCTATCTATCGCTGATAGCAGTCTTCGGGCTTTGCCTGCCCTTTCTCATCAAGAATTTCGGCTTGGCCCGGGAAAGCGCTGGCAACAAAAGGAAGGGGTCCATGTAGCTGCTGCGCCCGCCCCCTTCTTAGTCAATGGGGCAGCAGGTTCGGCATCCACTAGAAAAGAGAGAATCCACTTAGATAACCATGCCTCTGCTAGGCGGCAGCGTTCGAGAGTGGAGTTACGGAAAGAGCCGTCTGATGGAAAACAACTTTCACGTTCGGTTCAGAGAGCACTGTTTTCGTTGATCATTCCTCGTTCCCTTTCGTGTGAATTCCACAGCGGCGAATTCTCAACTTGTGGGGCCCTATCTATTCCATCTCTCAAGCCCCAAAGAAAAACCCCCTACCCTTCGGACTCCATATCTCTTTTGACTCTATATATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACACCATTCCTTTCTATTGCGCCTAAAATCTCTATTTTTGCTAATATTTCACGTCTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGTAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACTGGTTTCTCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTATCTTGATTTATGTATTAATGACAATAGATGCATTCGCTATAGTTTTAGCATTACGGCAACCTCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGTTGTTACCTTCTCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCGTTGGGCGGCCGGAAGGTTGCCACGAGTAAGTCAGTCCGTTTGGGAGACCGAAGACAGTTCTCCGTGCACCGGACACGTAGCTTACCGAATCCGTTGCGACACGGATGGGAATGCATGCTTCGAAAGATAG

Coding sequence (CDS)

ATGATCTATGGGTCTACTGGAGCTACCCACTTCGATCAATTAGCCAATATTTTGACCGGATACGAAATCACTGGTGCTCGATCTAGTGGTATTTTTATGGGGATTCTATCTATCGCTGATAGCAGTCTTCGGGCTTTGCCTGCCCTTTCTCATCAAGAATTTCGGCTTGGCCCGGGAAAGCGCTGGCAACAAAAGGAAGGGGTCCATGTAGCTGCTGCGCCCGCCCCCTTCTTAGTCAATGGGGCAGCAGGTTCGGCATCCACTAGAAAAGAGAGAATCCACTTAGATAACCATGCCTCTGCTAGGCGGCAGCGTTCGAGAGTGGAGTTACGGAAAGAGCCGTCTGATGGAAAACAACTTTCACGTTCGGTTCAGAGAGCACTGTTTTCGTTGATCATTCCTCGTTCCCTTTCGTGTGAATTCCACAGCGGCGAATTCTCAACTTGTGGGGCCCTATCTATTCCATCTCTCAAGCCCCAAAGAAAAACCCCCTACCCTTCGGACTCCATATCTCTTTTGACTCTATATATGTGGGCACCTGATATCTATGAGGGTTCACCCACCCCGGTTACACCATTCCTTTCTATTGCGCCTAAAATCTCTATTTTTGCTAATATTTCACGTCTTTCTATTTATGGTTCCTATGGAGCTACATTGCAACAAATCTTCTTTTTCTGTAGCATTGCTTCTATGATCTTAGGAGCACTGGCCGCCATGGCCCAAACGAAAGTCAAAAGACCTCTAGCTCATAGTTCAATTGGACATGTAGGTTATATTCGTACTGGTTTCTCATGTGGAACCATAGAAGGAATTCAATCACTACTAATTGGTATCTTGATTTATGTATTAATGACAATAGATGCATTCGCTATAGTTTTAGCATTACGGCAACCTCGTGTAAAATATATAGCGGATTTGGGCGCTCTAGCCAAAACGAATCCTATTTCGGTTGTTACCTTCTCCATTACTATGTTCTCATACGCAGGAATACCCCCGTTAGCCGGTTTTTGTAGCAAATTCTATTTGTTTTTCGCCGCTTTGGGTTGTGGGGCTTACTTCCTAGCCCCAGTGGGAGTAGTGACTAGCGTTATAGGTCGTTGGGCGGCCGGAAGGTTGCCACGAGTAAGTCAGTCCGTTTGGGAGACCGAAGACAGTTCTCCGTGCACCGGACACGTAGCTTACCGAATCCGTTGCGACACGGATGGGAATGCATGCTTCGAAAGATAG

Protein sequence

MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGKRWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQSVWETEDSSPCTGHVAYRIRCDTDGNACFER
Homology
BLAST of CmaCh12G006700 vs. ExPASy Swiss-Prot
Match: O05000 (NADH-ubiquinone oxidoreductase chain 2 OS=Arabidopsis thaliana OX=3702 GN=ND2 PE=1 SV=2)

HSP 1 Score: 347.8 bits (891), Expect = 1.6e-94
Identity = 214/365 (58.63%), Postives = 221/365 (60.55%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGK 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGIL IA   L  + A+           
Sbjct: 196 MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAV----------- 255

Query: 61  RWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQL 120
                          PF                                           
Sbjct: 256 ---------------PF------------------------------------------- 315

Query: 121 SRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAP 180
                                                                  +MWAP
Sbjct: 316 -------------------------------------------------------HMWAP 375

Query: 181 DIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMA 240
           DIYEGSPTPVT FLSIAPKISIFANI R+ IYGSYGATLQQIFFFCSIASMILGALAAMA
Sbjct: 376 DIYEGSPTPVTAFLSIAPKISIFANILRVFIYGSYGATLQQIFFFCSIASMILGALAAMA 435

Query: 241 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRV 300
           QTKVKR LA+SSIGHVGYI  GFSCGTIEGIQSLLIGI IY LMT+DAFAIVLALRQ RV
Sbjct: 436 QTKVKRLLAYSSIGHVGYICIGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVLALRQTRV 436

Query: 301 KYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 360
           KYIADLGALAKTNPI  +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLA VGVV
Sbjct: 496 KYIADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLALVGVV 436

Query: 361 TSVIG 366
           TSVIG
Sbjct: 556 TSVIG 436

BLAST of CmaCh12G006700 vs. ExPASy Swiss-Prot
Match: P93401 (NADH-ubiquinone oxidoreductase chain 2 OS=Oenothera berteroana OX=3950 GN=ND2 PE=2 SV=2)

HSP 1 Score: 344.7 bits (883), Expect = 1.4e-93
Identity = 212/365 (58.08%), Postives = 220/365 (60.27%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGK 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGIL IA   L  + A+           
Sbjct: 185 MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAV----------- 244

Query: 61  RWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQL 120
                          PF                                           
Sbjct: 245 ---------------PF------------------------------------------- 304

Query: 121 SRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAP 180
                                                                  +MWAP
Sbjct: 305 -------------------------------------------------------HMWAP 364

Query: 181 DIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMA 240
           DIYEGSPTPVT FLSIAPKISIFANI R+ IYGSYGATLQQIFFFCSIASMILGALAAMA
Sbjct: 365 DIYEGSPTPVTAFLSIAPKISIFANILRVFIYGSYGATLQQIFFFCSIASMILGALAAMA 424

Query: 241 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRV 300
           QTKVKR LA+SSIGHVGYI  GFSCGTIEGIQSLLIG+ IY L TI+AFAIVLALRQ RV
Sbjct: 425 QTKVKRLLAYSSIGHVGYICIGFSCGTIEGIQSLLIGLFIYALTTINAFAIVLALRQTRV 425

Query: 301 KYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 360
           KYIADLGALAKTNPI  +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLA VGVV
Sbjct: 485 KYIADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLASVGVV 425

Query: 361 TSVIG 366
           TSVIG
Sbjct: 545 TSVIG 425

BLAST of CmaCh12G006700 vs. ExPASy Swiss-Prot
Match: P15688 (NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris OX=161934 GN=ND2 PE=3 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 2.6e-84
Identity = 207/332 (62.35%), Postives = 220/332 (66.27%), Query Frame = 0

Query: 64  QKEGVHVAAAPAPFLVNGAAGSASTRKERIHLD-NHASARRQRS-----------RVELR 123
           +KEGVHVAAA APFLVN AAGSA+T KERIH    +ASA                +VE  
Sbjct: 92  RKEGVHVAAASAPFLVNRAAGSATTTKERIHFKITNASAMAACGMAGSDLFGYIIQVESG 151

Query: 124 KEPSDG---KQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSD 183
              + G        SVQRALFSL I RSL               SIPS  P  KTP P  
Sbjct: 152 VTGTAGLMENNFHGSVQRALFSLRILRSLRVN-SLARIQNFWGPSIPSSSP-AKTPLPFG 211

Query: 184 SISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSI 243
                  YMWAPDIYEGSPTPVT F SIAP+ SI ANI R+ IYGSYGATLQQIFFFCSI
Sbjct: 212 LNIFFDSYMWAPDIYEGSPTPVTAFFSIAPERSISANILRVFIYGSYGATLQQIFFFCSI 271

Query: 244 ASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTI---EGIQSLLIGILIYVLMT 303
           A + L +  AMA        A SSIG + Y    F    +   EGIQSLLIG+ IY  M 
Sbjct: 272 A-LRLRSTGAMA----NEGKASSSIGQLDYGGLYFVLVLMWNREGIQSLLIGLFIYASMD 331

Query: 304 IDAFAIVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFA 363
              FAIV ALRQ RVKYIADLGALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFA
Sbjct: 332 DRCFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFA 391

Query: 364 ALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ 378
           ALGCGAYFLAPVGVVTSVIG WAAGRLPRVSQ
Sbjct: 392 ALGCGAYFLAPVGVVTSVIGCWAAGRLPRVSQ 416

BLAST of CmaCh12G006700 vs. ExPASy Swiss-Prot
Match: P26846 (NADH-ubiquinone oxidoreductase chain 2 OS=Marchantia polymorpha OX=3197 GN=ND2 PE=3 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-78
Identity = 155/195 (79.49%), Postives = 168/195 (86.15%), Query Frame = 0

Query: 170 ISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIA 229
           I+ +  +MWAPD+YEGSPT VT F SIAPKISI AN+ R+ IY  Y  T QQ+FFFCSIA
Sbjct: 231 ITAVPFHMWAPDVYEGSPTIVTAFFSIAPKISILANMLRVFIYSFYDPTWQQLFFFCSIA 290

Query: 230 SMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAF 289
           SMILGALAAMAQ KVKR LA+SSIGHVGY+  GFSCGTIEGIQSLLIGI IYVLMT++ F
Sbjct: 291 SMILGALAAMAQNKVKRLLAYSSIGHVGYLLIGFSCGTIEGIQSLLIGIFIYVLMTVNVF 350

Query: 290 AIVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGC 349
           AIVLALRQ R KYIADLGALAKTNPI  +T SITMFSYAGIPPLAGFCSKFYLFFAALGC
Sbjct: 351 AIVLALRQNRFKYIADLGALAKTNPILAITLSITMFSYAGIPPLAGFCSKFYLFFAALGC 410

Query: 350 GAYFLAPVGVVTSVI 365
           GAY LA +GVVTSVI
Sbjct: 411 GAYLLALIGVVTSVI 425

BLAST of CmaCh12G006700 vs. ExPASy Swiss-Prot
Match: Q2RU27 (NADH-quinone oxidoreductase subunit N OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=nuoN PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.1e-42
Identity = 89/199 (44.72%), Postives = 133/199 (66.83%), Query Frame = 0

Query: 170 ISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSI--YGSYGATLQQIFFFCS 229
           +S +  +MWAPD+YEG+PTPVT F ++APKI+     +R+ +  + +Y    QQ+    S
Sbjct: 222 VSAVPFHMWAPDVYEGAPTPVTAFFAVAPKIAALTLFARVMMGPFAAYADQWQQVIILIS 281

Query: 230 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTID 289
           I SM+LG  AA+ QT +KR +A+SSIGHVGY   G + GT EG++ +L+ + IY+ M + 
Sbjct: 282 ILSMLLGGFAAIVQTNIKRLMAYSSIGHVGYALIGIAAGTTEGVRGVLVYLAIYLFMNVG 341

Query: 290 AFAIVLALRQP--RVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFA 349
            F ++LA+RQ    V+ I DL  L+K +P+     ++ MFS AG+PPLAGF  KFY+F A
Sbjct: 342 TFTVILAMRQKGRAVEGINDLAGLSKQHPMMAAAMAVFMFSMAGVPPLAGFFGKFYVFMA 401

Query: 350 ALGCGAYFLAPVGVVTSVI 365
           A+  G + LA +GV++SV+
Sbjct: 402 AVNSGLFALAVIGVLSSVV 420

BLAST of CmaCh12G006700 vs. ExPASy TrEMBL
Match: A0A2P6P115 (Putative NADH:ubiquinone reductase (H(+)-translocating) OS=Rosa chinensis OX=74649 GN=RchiOBHm_MTg0498511 PE=4 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.3e-139
Identity = 274/315 (86.98%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 64  QKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRS 123
           +KEGVHVAAAPAPFLVNGAAGSAST KERI      S     S VELR++PSDGKQLSRS
Sbjct: 38  RKEGVHVAAAPAPFLVNGAAGSASTIKERIRF---LSEPLLGSVVELREQPSDGKQLSRS 97

Query: 124 VQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSL-KPQRKTPYPSDSISLLTLYMWAPDI 183
           VQRALFSL IPRSLSCEF   EF TCGALSI SL +  +KTP PSDS SLLTLYMWAPDI
Sbjct: 98  VQRALFSLRIPRSLSCEFPCNEFQTCGALSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDI 157

Query: 184 YEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 243
           YEGSPTPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQT
Sbjct: 158 YEGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 217

Query: 244 KVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKY 303
           KVKRPLAHSSIGHVGYIRTG SCGTIEGIQSLLIG+ IY LMTIDAFAIVLALRQ RVKY
Sbjct: 218 KVKRPLAHSSIGHVGYIRTGLSCGTIEGIQSLLIGLFIYALMTIDAFAIVLALRQTRVKY 277

Query: 304 IADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 363
           IADLGALAKTNPIS +TF+ITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS
Sbjct: 278 IADLGALAKTNPISAITFAITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 337

Query: 364 VIGRWAAGRLPRVSQ 378
           VIGRWAAGRLPRVS+
Sbjct: 338 VIGRWAAGRLPRVSK 349

BLAST of CmaCh12G006700 vs. ExPASy TrEMBL
Match: A0A5H2XRW6 (NADH-Ubiquinone/plastoquinone complex I protein OS=Prunus dulcis OX=3755 GN=Prudu_950S000900 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 9.5e-138
Identity = 292/429 (68.07%), Postives = 311/429 (72.49%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMG----------ILSIADSSLRALPALS 60
           MIYGSTGATHFDQLA ILTGYEITGARSSG   G          IL +     R  P ++
Sbjct: 14  MIYGSTGATHFDQLAKILTGYEITGARSSGSTKGGGRKTLSEIEILFVFHRK-RRRPRMA 73

Query: 61  H-----------------------------------------QEFRLGPGKRWQQKEGVH 120
           +                                         ++FRLGPGKRWQQ++G  
Sbjct: 74  YGAYYLRGTRFFDRGSMIAGPSPRSARWPIGIAAFGLCLPLKKKFRLGPGKRWQQQKGRG 133

Query: 121 VAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRSVQRALF 180
             +  A  L++                      +  SRVELR++PSDGK LSRSVQRALF
Sbjct: 134 PCSCCARPLLS----------------------QWGSRVELREQPSDGKLLSRSVQRALF 193

Query: 181 SLIIPRSLSCEFHSGEFSTCGA-LSIPSL-KPQRKTPYPSDSISLLTLYMWAPDIYEGSP 240
           SL IPRSLSC+F   E  TCGA LSI SL +  +KTP PSDS SLLTLYMWAPDIYEGSP
Sbjct: 194 SLRIPRSLSCKFPCNELKTCGALLSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDIYEGSP 253

Query: 241 TPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP 300
           TPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP
Sbjct: 254 TPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP 313

Query: 301 LAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKYIADLG 360
           LAHSSIGHVGYIRTG SCGTIEGIQSLLIGI IY LMTIDAFAIVLALRQ RVKYIADLG
Sbjct: 314 LAHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYALMTIDAFAIVLALRQTRVKYIADLG 373

Query: 361 ALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW 377
           ALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW
Sbjct: 374 ALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW 419

BLAST of CmaCh12G006700 vs. ExPASy TrEMBL
Match: A0A6N2L074 (Proton_antipo_M domain-containing protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS122732 PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 5.8e-135
Identity = 258/282 (91.49%), Postives = 267/282 (94.68%), Query Frame = 0

Query: 96  DNHASARRQRSRVELRKEPSDGKQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIP 155
           DNHAS+ RQRSRVELR++PSDGK+LSRSVQRALFSL IPRSLSCEF SGEF TCGALSIP
Sbjct: 17  DNHASS-RQRSRVELREQPSDGKRLSRSVQRALFSLKIPRSLSCEFPSGEFKTCGALSIP 76

Query: 156 SLKPQRKTPYPSDSISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSY 215
           SL+P+ K P PSDS+ LLTLYMWAPDIYEGSPTPVT FLSIAPKISI ANISR+SIYGSY
Sbjct: 77  SLEPRIKPPSPSDSLFLLTLYMWAPDIYEGSPTPVTAFLSIAPKISISANISRVSIYGSY 136

Query: 216 GATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLL 275
           GATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLL
Sbjct: 137 GATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLL 196

Query: 276 IGILIYVLMTIDAFAIVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAG 335
           IGI IY LMTIDAFAIV ALRQ RVKYIADLGALAKTNPIS +TFSITMFSYAGIPPLAG
Sbjct: 197 IGIFIYALMTIDAFAIVSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAG 256

Query: 336 FCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ 378
           FCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ
Sbjct: 257 FCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ 297

BLAST of CmaCh12G006700 vs. ExPASy TrEMBL
Match: A0A5N5IZ41 (Proton_antipo_M domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_030085 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 8.1e-129
Identity = 244/266 (91.73%), Postives = 252/266 (94.74%), Query Frame = 0

Query: 112 KEPSDGKQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSIS 171
           ++PSDGK+LSRSVQRALFSL IPRSLSCEF SGEF TCGALSIPSL+P+ K P PSDS+ 
Sbjct: 3   QQPSDGKRLSRSVQRALFSLKIPRSLSCEFPSGEFKTCGALSIPSLEPRIKPPSPSDSLF 62

Query: 172 LLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASM 231
           LLTLYMWAPDIYEGSPTPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASM
Sbjct: 63  LLTLYMWAPDIYEGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASM 122

Query: 232 ILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAI 291
           ILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGI IY LMTIDAFAI
Sbjct: 123 ILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTIDAFAI 182

Query: 292 VLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 351
           V ALRQ RVKYIADLGALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA
Sbjct: 183 VSALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGA 242

Query: 352 YFLAPVGVVTSVIGRWAAGRLPRVSQ 378
           YFLAPVGVVTSVIGRWAAGRLPRVSQ
Sbjct: 243 YFLAPVGVVTSVIGRWAAGRLPRVSQ 268

BLAST of CmaCh12G006700 vs. ExPASy TrEMBL
Match: A0A4D8YAJ0 (NADH-quinone oxidoreductase subunit N OS=Salvia splendens OX=180675 GN=nuoN PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 6.9e-128
Identity = 294/505 (58.22%), Postives = 300/505 (59.41%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIA--------------------- 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGIL IA                     
Sbjct: 14  MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAVPFRAAVGRTAA 73

Query: 61  -----DSSLRALPALSHQ------------------------------------------ 120
                D S      LSH                                           
Sbjct: 74  YRCMLDESAAFSVVLSHSCPPRRATRSVVLSHKISTPPACWPGESKFSSGQLSTQSSAKN 133

Query: 121 -----------------------EFR--------------LGPGKRW------------- 180
                                  +FR              LG  K+              
Sbjct: 134 TVESRNARCWCASCSQGKKSDKVQFRFDCLQHGSRLPKKSLGTMKKKDISPSGFACPLQD 193

Query: 181 ----------QQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRK 240
                      +KEGVHVAAAPAPFLVNGA                              
Sbjct: 194 SGSARESAGNNRKEGVHVAAAPAPFLVNGAQ----------------------------- 253

Query: 241 EPSDGKQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISL 300
                       QRALFSL IPRSLSCEF SGEF TCGALSIPSL+P+RK P PSDSISL
Sbjct: 254 ------------QRALFSLRIPRSLSCEFPSGEFKTCGALSIPSLEPRRKPPSPSDSISL 313

Query: 301 LTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMI 360
           LTLYMWAPDIYEGSPTPVT FLSIAPKISI ANISRLSIYGSYGATLQQIFFFCSIASMI
Sbjct: 314 LTLYMWAPDIYEGSPTPVTAFLSIAPKISISANISRLSIYGSYGATLQQIFFFCSIASMI 373

Query: 361 LGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIV 378
           LGALAAMAQTKVKR LAHSSIGHVGYIR GFSCGTIEGIQSLLIGI IY  MTIDAFAIV
Sbjct: 374 LGALAAMAQTKVKRLLAHSSIGHVGYIRIGFSCGTIEGIQSLLIGIFIYASMTIDAFAIV 433

BLAST of CmaCh12G006700 vs. NCBI nr
Match: KAG7022596.1 (NADH-ubiquinone oxidoreductase chain 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 596.3 bits (1536), Expect = 2.0e-166
Identity = 315/371 (84.91%), Postives = 323/371 (87.06%), Query Frame = 0

Query: 40  DSSLRALPAL--SHQEFRLGPGKRWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDN 99
           DSSLRALPAL  +HQEFRLGPGKRWQQKEGVHVAAAPAPFLVNG+AGSAST         
Sbjct: 39  DSSLRALPALDHTHQEFRLGPGKRWQQKEGVHVAAAPAPFLVNGSAGSASTS-------- 98

Query: 100 HASARRQRSRVELRKEPSDGKQLSRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSL 159
                      E R+  SD   L     RALFSLIIPRSLS  FHSGEFSTCGALSIPSL
Sbjct: 99  -----------EKRESTSDNHGLCLLGCRALFSLIIPRSLSSSFHSGEFSTCGALSIPSL 158

Query: 160 KPQRKTPYPSDSISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGA 219
           KP+RK P PSDSISLLTLYMWAP+IYEGSPTPVT FL  APKISIFANISRLSIYGSYGA
Sbjct: 159 KPRRKPPSPSDSISLLTLYMWAPNIYEGSPTPVTAFLPFAPKISIFANISRLSIYGSYGA 218

Query: 220 TLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIG 279
           TLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRT FSCGTIEGIQSLLIG
Sbjct: 219 TLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTSFSCGTIEGIQSLLIG 278

Query: 280 ILIYVLMTIDAFAIVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFC 339
           I IYV MTIDAFAIVLALRQ RVKY+ADLGALAKT+PIS +TFSITMFSYAGIPPLAGFC
Sbjct: 279 IFIYVFMTIDAFAIVLALRQTRVKYLADLGALAKTHPISAITFSITMFSYAGIPPLAGFC 338

Query: 340 SKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQSVWETEDSSPCTGHVAYRIR 399
           SKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQSVWETE SSPCTGHVAYRI 
Sbjct: 339 SKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQSVWETEGSSPCTGHVAYRIS 390

Query: 400 CDTDGNACFER 409
           CDTDGNAC ER
Sbjct: 399 CDTDGNACSER 390

BLAST of CmaCh12G006700 vs. NCBI nr
Match: PRQ15618.1 (putative NADH:ubiquinone reductase (H(+)-translocating) [Rosa chinensis])

HSP 1 Score: 506.1 bits (1302), Expect = 2.8e-139
Identity = 274/315 (86.98%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 64  QKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRS 123
           +KEGVHVAAAPAPFLVNGAAGSAST KERI      S     S VELR++PSDGKQLSRS
Sbjct: 38  RKEGVHVAAAPAPFLVNGAAGSASTIKERIRF---LSEPLLGSVVELREQPSDGKQLSRS 97

Query: 124 VQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSL-KPQRKTPYPSDSISLLTLYMWAPDI 183
           VQRALFSL IPRSLSCEF   EF TCGALSI SL +  +KTP PSDS SLLTLYMWAPDI
Sbjct: 98  VQRALFSLRIPRSLSCEFPCNEFQTCGALSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDI 157

Query: 184 YEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 243
           YEGSPTPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQT
Sbjct: 158 YEGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 217

Query: 244 KVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKY 303
           KVKRPLAHSSIGHVGYIRTG SCGTIEGIQSLLIG+ IY LMTIDAFAIVLALRQ RVKY
Sbjct: 218 KVKRPLAHSSIGHVGYIRTGLSCGTIEGIQSLLIGLFIYALMTIDAFAIVLALRQTRVKY 277

Query: 304 IADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 363
           IADLGALAKTNPIS +TF+ITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS
Sbjct: 278 IADLGALAKTNPISAITFAITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 337

Query: 364 VIGRWAAGRLPRVSQ 378
           VIGRWAAGRLPRVS+
Sbjct: 338 VIGRWAAGRLPRVSK 349

BLAST of CmaCh12G006700 vs. NCBI nr
Match: KAG8362758.1 (hypothetical protein BUALT_BualtUnG0039300 [Buddleja alternifolia] >KAG8363176.1 hypothetical protein BUALT_BualtMtG0005900 [Buddleja alternifolia])

HSP 1 Score: 500.4 bits (1287), Expect = 1.5e-137
Identity = 268/314 (85.35%), Postives = 277/314 (88.22%), Query Frame = 0

Query: 64  QKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRS 123
           +KEGVHVAAAPAPFLVNGA                     Q+SRVELR++PSDGK LSRS
Sbjct: 488 RKEGVHVAAAPAPFLVNGA---------------------QQSRVELREQPSDGKLLSRS 547

Query: 124 VQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAPDIY 183
           VQRALFSL IPRSLSCEF SGEF TCGALSIPSL+P+RK P PSDSISLLTLYMWAPDIY
Sbjct: 548 VQRALFSLRIPRSLSCEFPSGEFKTCGALSIPSLEPRRKPPSPSDSISLLTLYMWAPDIY 607

Query: 184 EGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 243
           EGSPTPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQTK
Sbjct: 608 EGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 667

Query: 244 VKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKYI 303
           VKR LAHSSIGHVGYIRTGFSCGTIEGIQSLLIGI IY  MTIDAFAIV ALRQ RVKYI
Sbjct: 668 VKRLLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYASMTIDAFAIVSALRQTRVKYI 727

Query: 304 ADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSV 363
           ADLGALAKTNPI  +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSV
Sbjct: 728 ADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSV 780

Query: 364 IGRWAAGRLPRVSQ 378
           IGRWAAGRLPRVS+
Sbjct: 788 IGRWAAGRLPRVSK 780

BLAST of CmaCh12G006700 vs. NCBI nr
Match: KAG8362758.1 (hypothetical protein BUALT_BualtUnG0039300 [Buddleja alternifolia] >KAG8363176.1 hypothetical protein BUALT_BualtMtG0005900 [Buddleja alternifolia])

HSP 1 Score: 75.9 bits (185), Expect = 9.2e-10
Identity = 42/60 (70.00%), Postives = 45/60 (75.00%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGK 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGIL IA   L  + A+    FR   G+
Sbjct: 238 MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAV---PFRAAVGR 294


HSP 2 Score: 500.0 bits (1286), Expect = 2.0e-137
Identity = 292/429 (68.07%), Postives = 311/429 (72.49%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMG----------ILSIADSSLRALPALS 60
           MIYGSTGATHFDQLA ILTGYEITGARSSG   G          IL +     R  P ++
Sbjct: 14  MIYGSTGATHFDQLAKILTGYEITGARSSGSTKGGGRKTLSEIEILFVFHRK-RRRPRMA 73

Query: 61  H-----------------------------------------QEFRLGPGKRWQQKEGVH 120
           +                                         ++FRLGPGKRWQQ++G  
Sbjct: 74  YGAYYLRGTRFFDRGSMIAGPSPRSARWPIGIAAFGLCLPLKKKFRLGPGKRWQQQKGRG 133

Query: 121 VAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRSVQRALF 180
             +  A  L++                      +  SRVELR++PSDGK LSRSVQRALF
Sbjct: 134 PCSCCARPLLS----------------------QWGSRVELREQPSDGKLLSRSVQRALF 193

Query: 181 SLIIPRSLSCEFHSGEFSTCGA-LSIPSL-KPQRKTPYPSDSISLLTLYMWAPDIYEGSP 240
           SL IPRSLSC+F   E  TCGA LSI SL +  +KTP PSDS SLLTLYMWAPDIYEGSP
Sbjct: 194 SLRIPRSLSCKFPCNELKTCGALLSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDIYEGSP 253

Query: 241 TPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP 300
           TPVT FLSIAPKISI ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP
Sbjct: 254 TPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRP 313

Query: 301 LAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKYIADLG 360
           LAHSSIGHVGYIRTG SCGTIEGIQSLLIGI IY LMTIDAFAIVLALRQ RVKYIADLG
Sbjct: 314 LAHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYALMTIDAFAIVLALRQTRVKYIADLG 373

Query: 361 ALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW 377
           ALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW
Sbjct: 374 ALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRW 419

BLAST of CmaCh12G006700 vs. NCBI nr
Match: KAF9660721.1 (hypothetical protein SADUNF_SadunfMtG0001800 [Salix dunnii])

HSP 1 Score: 480.7 bits (1236), Expect = 1.2e-131
Identity = 253/293 (86.35%), Postives = 266/293 (90.78%), Query Frame = 0

Query: 85  SASTRKERIHLDNHASARRQRSRVELRKEPSDGKQLSRSVQRALFSLIIPRSLSCEFHSG 144
           + + RKE +H+       +  SRVELR++PSDGK+LSRSVQRALFSL IPRSLSCEF SG
Sbjct: 260 AGNNRKEGVHVAARPLLSQWGSRVELREQPSDGKRLSRSVQRALFSLKIPRSLSCEFPSG 319

Query: 145 EFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFA 204
           E  TCGALSIPSL+P+ K P PSDS+ LLTLYMWAPDIYEGSPTPVT FLSIAPKISI A
Sbjct: 320 ELKTCGALSIPSLEPRIKPPSPSDSLFLLTLYMWAPDIYEGSPTPVTAFLSIAPKISISA 379

Query: 205 NISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFS 264
           NISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFS
Sbjct: 380 NISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFS 439

Query: 265 CGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRVKYIADLGALAKTNPISVVTFSITM 324
           CGTIEGIQSLLIGI IY LMTIDAFAIV ALRQ RVKYIADLGALAKTNPIS +TFSITM
Sbjct: 440 CGTIEGIQSLLIGIFIYALMTIDAFAIVSALRQTRVKYIADLGALAKTNPISAITFSITM 499

Query: 325 FSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ 378
           FSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ
Sbjct: 500 FSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQ 552

BLAST of CmaCh12G006700 vs. TAIR 10
Match: ATMG00285.1 (NADH dehydrogenase 2A )

HSP 1 Score: 367.5 bits (942), Expect = 1.4e-101
Identity = 223/372 (59.95%), Postives = 230/372 (61.83%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGK 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGILSIA   L  + A+           
Sbjct: 196 MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILSIAVGFLFKITAV----------- 255

Query: 61  RWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQL 120
                          PF                                           
Sbjct: 256 ---------------PF------------------------------------------- 315

Query: 121 SRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAP 180
                                                                  +MWAP
Sbjct: 316 -------------------------------------------------------HMWAP 375

Query: 181 DIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMA 240
           DIYEGSPTPVT FLSIAPKISI ANI R+SIYGSYGATLQQIFFFCSIASMILGALAAMA
Sbjct: 376 DIYEGSPTPVTAFLSIAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGALAAMA 435

Query: 241 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRV 300
           QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGI IY LMT+DAFAIV ALRQ RV
Sbjct: 436 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALRQTRV 443

Query: 301 KYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 360
           KYIADLGALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV
Sbjct: 496 KYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 443

Query: 361 TSVIGRWAAGRL 373
           TSVIGR+   RL
Sbjct: 556 TSVIGRFYYIRL 443

BLAST of CmaCh12G006700 vs. TAIR 10
Match: ATMG01320.1 (NADH dehydrogenase 2B )

HSP 1 Score: 367.5 bits (942), Expect = 1.4e-101
Identity = 223/372 (59.95%), Postives = 230/372 (61.83%), Query Frame = 0

Query: 1   MIYGSTGATHFDQLANILTGYEITGARSSGIFMGILSIADSSLRALPALSHQEFRLGPGK 60
           MIYGSTGATHFDQLA ILTGYEITGARSSGIFMGILSIA   L  + A+           
Sbjct: 196 MIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILSIAVGFLFKITAV----------- 255

Query: 61  RWQQKEGVHVAAAPAPFLVNGAAGSASTRKERIHLDNHASARRQRSRVELRKEPSDGKQL 120
                          PF                                           
Sbjct: 256 ---------------PF------------------------------------------- 315

Query: 121 SRSVQRALFSLIIPRSLSCEFHSGEFSTCGALSIPSLKPQRKTPYPSDSISLLTLYMWAP 180
                                                                  +MWAP
Sbjct: 316 -------------------------------------------------------HMWAP 375

Query: 181 DIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMA 240
           DIYEGSPTPVT FLSIAPKISI ANI R+SIYGSYGATLQQIFFFCSIASMILGALAAMA
Sbjct: 376 DIYEGSPTPVTAFLSIAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGALAAMA 435

Query: 241 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALRQPRV 300
           QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGI IY LMT+DAFAIV ALRQ RV
Sbjct: 436 QTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALRQTRV 443

Query: 301 KYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 360
           KYIADLGALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV
Sbjct: 496 KYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVV 443

Query: 361 TSVIGRWAAGRL 373
           TSVIGR+   RL
Sbjct: 556 TSVIGRFYYIRL 443

BLAST of CmaCh12G006700 vs. TAIR 10
Match: AT2G07689.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 364.8 bits (935), Expect = 9.2e-101
Identity = 188/201 (93.53%), Postives = 193/201 (96.02%), Query Frame = 0

Query: 177 MWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQQIFFFCSIASMILGAL 236
           MWAPDIYEGSPTPVT FLSIAPKISI ANI R+SIYGSYGATLQQIFFFCSIASMILGAL
Sbjct: 1   MWAPDIYEGSPTPVTAFLSIAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGAL 60

Query: 237 AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGILIYVLMTIDAFAIVLALR 296
           AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGI IY LMT+DAFAIV ALR
Sbjct: 61  AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALR 120

Query: 297 QPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAP 356
           Q RVKYIADLGALAKTNPIS +TFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAP
Sbjct: 121 QTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAP 180

Query: 357 VGVVTSVIGRWAAGRLPRVSQ 378
           VGVVTSVIGRWAAGRLPR+S+
Sbjct: 181 VGVVTSVIGRWAAGRLPRISK 201

BLAST of CmaCh12G006700 vs. TAIR 10
Match: ATCG00890.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 119.4 bits (298), Expect = 6.7e-27
Identity = 73/199 (36.68%), Postives = 112/199 (56.28%), Query Frame = 0

Query: 170 ISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQ--QIFFFCS 229
           +SL   + W PD+YEGSPTPV  FLS+  K++  A+ +R+     Y ++ +   +    +
Sbjct: 121 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDIPFYFSSNEWHLLLEILA 180

Query: 230 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIE-GIQSLLIGILIYVLMTI 289
           I SMI G L A+ QT +KR LA+SSIG +GY+  G   G    G  S++  +L Y+ M +
Sbjct: 181 ILSMIFGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNGGYASMITYMLFYIAMNL 240

Query: 290 DAFA-IVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFA 349
             FA I+L   +     I D   L   +P   ++ ++ + S  G+PPLAGF  K +LF+ 
Sbjct: 241 GTFACIILFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWC 300

Query: 350 ALGCGAYFLAPVGVVTSVI 365
               G YFL  +G++TSV+
Sbjct: 301 GWQAGLYFLVSIGLLTSVL 319

BLAST of CmaCh12G006700 vs. TAIR 10
Match: ATCG01250.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 119.4 bits (298), Expect = 6.7e-27
Identity = 73/199 (36.68%), Postives = 112/199 (56.28%), Query Frame = 0

Query: 170 ISLLTLYMWAPDIYEGSPTPVTPFLSIAPKISIFANISRLSIYGSYGATLQ--QIFFFCS 229
           +SL   + W PD+YEGSPTPV  FLS+  K++  A+ +R+     Y ++ +   +    +
Sbjct: 121 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDIPFYFSSNEWHLLLEILA 180

Query: 230 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIE-GIQSLLIGILIYVLMTI 289
           I SMI G L A+ QT +KR LA+SSIG +GY+  G   G    G  S++  +L Y+ M +
Sbjct: 181 ILSMIFGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNGGYASMITYMLFYIAMNL 240

Query: 290 DAFA-IVLALRQPRVKYIADLGALAKTNPISVVTFSITMFSYAGIPPLAGFCSKFYLFFA 349
             FA I+L   +     I D   L   +P   ++ ++ + S  G+PPLAGF  K +LF+ 
Sbjct: 241 GTFACIILFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWC 300

Query: 350 ALGCGAYFLAPVGVVTSVI 365
               G YFL  +G++TSV+
Sbjct: 301 GWQAGLYFLVSIGLLTSVL 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O050001.6e-9458.63NADH-ubiquinone oxidoreductase chain 2 OS=Arabidopsis thaliana OX=3702 GN=ND2 PE... [more]
P934011.4e-9358.08NADH-ubiquinone oxidoreductase chain 2 OS=Oenothera berteroana OX=3950 GN=ND2 PE... [more]
P156882.6e-8462.35NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris OX=161934 GN=ND2 PE=3 SV... [more]
P268461.3e-7879.49NADH-ubiquinone oxidoreductase chain 2 OS=Marchantia polymorpha OX=3197 GN=ND2 P... [more]
Q2RU271.1e-4244.72NADH-quinone oxidoreductase subunit N OS=Rhodospirillum rubrum (strain ATCC 1117... [more]
Match NameE-valueIdentityDescription
A0A2P6P1151.3e-13986.98Putative NADH:ubiquinone reductase (H(+)-translocating) OS=Rosa chinensis OX=746... [more]
A0A5H2XRW69.5e-13868.07NADH-Ubiquinone/plastoquinone complex I protein OS=Prunus dulcis OX=3755 GN=Prud... [more]
A0A6N2L0745.8e-13591.49Proton_antipo_M domain-containing protein OS=Salix viminalis OX=40686 GN=SVIM_LO... [more]
A0A5N5IZ418.1e-12991.73Proton_antipo_M domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38... [more]
A0A4D8YAJ06.9e-12858.22NADH-quinone oxidoreductase subunit N OS=Salvia splendens OX=180675 GN=nuoN PE=4... [more]
Match NameE-valueIdentityDescription
KAG7022596.12.0e-16684.91NADH-ubiquinone oxidoreductase chain 2, partial [Cucurbita argyrosperma subsp. a... [more]
PRQ15618.12.8e-13986.98putative NADH:ubiquinone reductase (H(+)-translocating) [Rosa chinensis][more]
KAG8362758.11.5e-13785.35hypothetical protein BUALT_BualtUnG0039300 [Buddleja alternifolia] >KAG8363176.1... [more]
KAG8362758.19.2e-1070.00hypothetical protein BUALT_BualtUnG0039300 [Buddleja alternifolia] >KAG8363176.1... [more]
KAF9660721.11.2e-13186.35hypothetical protein SADUNF_SadunfMtG0001800 [Salix dunnii][more]
Match NameE-valueIdentityDescription
ATMG00285.11.4e-10159.95NADH dehydrogenase 2A [more]
ATMG01320.11.4e-10159.95NADH dehydrogenase 2B [more]
AT2G07689.19.2e-10193.53NADH-Ubiquinone/plastoquinone (complex I) protein [more]
ATCG00890.16.7e-2736.68NADH-Ubiquinone/plastoquinone (complex I) protein [more]
ATCG01250.16.7e-2736.68NADH-Ubiquinone/plastoquinone (complex I) protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001750NADH:quinone oxidoreductase/Mrp antiporter, membrane subunitPFAMPF00361Proton_antipo_Mcoord: 171..365
e-value: 3.1E-51
score: 174.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..118
NoneNo IPR availablePANTHERPTHR22773NADH DEHYDROGENASEcoord: 1..376
NoneNo IPR availablePANTHERPTHR22773:SF41NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2coord: 1..376

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G006700.1CmaCh12G006700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0009579 thylakoid
molecular_function GO:0016491 oxidoreductase activity