CmaCh11G011710 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G011710
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	pectinesterase inhibitor-like
Location	Cma_Chr11: 6822379 .. 6822846 (+)
RNA-Seq Expression	CmaCh11G011710
Synteny	CmaCh11G011710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATGCAGCTTCCCAAAACGACGTCGCTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTGAAAGGACTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGCTGAAACTCGTGCCCTCGCCCAGTCCTTGACGTCCAAGGCAGCCGATCCCAAGCTTAAAGAGCGCTATGCCACCTGCGCTGAACTCTACGACAATGCCGCCGGCGACATCGAGGATGGGAAGGTCAACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCATTTGCAGCCATGACGGAGGCTGGCGACTGTCTGGACAGCTTTGCGCAGCCGCCGAAGGACCCATCGGCGCTGTCGGGCAATGGCAAGACTGTTGAAGATATTTGTAGTATCATCTTGGTTATAGCCAACCTTCTTCTTGGGCGTGTCTAA

mRNA sequence

ATGCATGCAGCTTCCCAAAACGACGTCGCTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTGAAAGGACTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGCTGAAACTCGTGCCCTCGCCCAGTCCTTGACGTCCAAGGCAGCCGATCCCAAGCTTAAAGAGCGCTATGCCACCTGCGCTGAACTCTACGACAATGCCGCCGGCGACATCGAGGATGGGAAGGTCAACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCATTTGCAGCCATGACGGAGGCTGGCGACTGTCTGGACAGCTTTGCGCAGCCGCCGAAGGACCCATCGGCGCTGTCGGGCAATGGCAAGACTGTTGAAGATATTTGTAGTATCATCTTGGTTATAGCCAACCTTCTTCTTGGGCGTGTCTAA

Coding sequence (CDS)

ATGCATGCAGCTTCCCAAAACGACGTCGCTTCCACCATCTGCAAGAAAACTAGAAACCCTTCTTTTTGCTTTAACGTGTTGAAATCTGCTGGCACCACAGACTTGAAAGGACTGGCCACCTTCACCCTCAACCTCGCCCACGACAAGGTTGCTGAAACTCGTGCCCTCGCCCAGTCCTTGACGTCCAAGGCAGCCGATCCCAAGCTTAAAGAGCGCTATGCCACCTGCGCTGAACTCTACGACAATGCCGCCGGCGACATCGAGGATGGGAAGGTCAACTTGGGGGAAGGTGACTACAATGGCGTCAACATTAAGGCATTTGCAGCCATGACGGAGGCTGGCGACTGTCTGGACAGCTTTGCGCAGCCGCCGAAGGACCCATCGGCGCTGTCGGGCAATGGCAAGACTGTTGAAGATATTTGTAGTATCATCTTGGTTATAGCCAACCTTCTTCTTGGGCGTGTCTAA

Protein sequence

MHAASQNDVASTICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVAETRALAQSLTSKAADPKLKERYATCAELYDNAAGDIEDGKVNLGEGDYNGVNIKAFAAMTEAGDCLDSFAQPPKDPSALSGNGKTVEDICSIILVIANLLLGRV

Homology

[Close]

BLAST of CmaCh11G011710 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 3.9e-28
Identity = 69/152 (45.39%), Postives = 92/152 (60.53%), Query Frame = 0


Query: 5   SQNDVASTICKKTRNPSFCFNVLKS---AGTTDLKGLATFTLNLAHDKVAETRALAQSLT 64
           ++N + S IC KTRNPS C   L+S   + + DLKGL  F++++A     +T  +  SLT
Sbjct: 32  AENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLT 91

Query: 65  SKAADPKLKERYATCAELYDNAAGDIEDGKVNLGEGDYNGVNIKAFAAMTEAGDCLDSFA 124
           ++A DPKLK RY TC+E Y +A   +   K  L  GDYN +NI A AA   AG C DSF 
Sbjct: 92  NQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFE 151

Query: 125 QPPKDPSALSGNGKTVEDICSIILVIANLLLG 154
            PP  P+ L      +ED+C I+LVI+NLL G
Sbjct: 152 GPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of CmaCh11G011710 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-18
Identity = 52/142 (36.62%), Postives = 81/142 (57.04%), Query Frame = 0


Query: 11  STICKKTRNPSFCFNVLKSA-GTTDLKGLATFTLNLAHDKVAETRALAQSLTSKAADPKL 70
           STIC KT NPSFC   L +   + +L+ LA  TL+    +  +T    QS+     DP+ 
Sbjct: 32  STICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRS 91

Query: 71  KERYATCAELYDNAAGDIEDGKVNLGEGDYNGVNIKAFAAMTEAGDCLDSFAQPPKDPSA 130
           K  Y +C + Y++A G++E+   +L  GD  G+N+K  AA+  A  CLD   +     S+
Sbjct: 92  KLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDSS 151

Query: 131 LSGNGKTVEDICSIILVIANLL 152
           +  N KT++++C I LVI+N+L
Sbjct: 152 VVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmaCh11G011710 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 94.4 bits (233), Expect = 8.8e-20
Identity = 52/142 (36.62%), Postives = 81/142 (57.04%), Query Frame = 0


Query: 11  STICKKTRNPSFCFNVLKSA-GTTDLKGLATFTLNLAHDKVAETRALAQSLTSKAADPKL 70
           STIC KT NPSFC   L +   + +L+ LA  TL+    +  +T    QS+     DP+ 
Sbjct: 32  STICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRS 91

Query: 71  KERYATCAELYDNAAGDIEDGKVNLGEGDYNGVNIKAFAAMTEAGDCLDSFAQPPKDPSA 130
           K  Y +C + Y++A G++E+   +L  GD  G+N+K  AA+  A  CLD   +     S+
Sbjct: 92  KLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSVDSS 151

Query: 131 LSGNGKTVEDICSIILVIANLL 152
           +  N KT++++C I LVI+N+L
Sbjct: 152 VVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmaCh11G011710 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.6e-05
Identity = 42/142 (29.58%), Postives = 64/142 (45.07%), Query Frame = 0


Query: 12  TICKKTRNPSFCFNVLKSAGTTDLKGLATFTLNLAHDKVAETRALAQSLTSKAADPKLKE 71
           TIC KT NP  C + +    T  LK   +  L L         A+  S+T+  A   + E
Sbjct: 132 TICGKTDNPPLCESSVSPLLTPQLKPNTSSVLIL---------AIQASITATKAAMAIVE 191

Query: 72  RY--ATCAELYDNAAGDIEDGKVNLGEGDYNGVNIKAFAAMTEAGDCLDSFAQPPKDPSA 131
           +   + C ELYD+A  ++ED    +   D   VN    AAMT+   C D F +   +P+ 
Sbjct: 192 KVDASDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGF-EESGEPNP 251

Query: 132 LSGNGKTVEDICSIILVIANLL 152
           L+     +  + S  L I+ L+
Sbjct: 252 LAYVADKLTKMVSNCLAISTLI 263

The following BLAST results are available for this feature:

BLAST of CmaCh11G011710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	3.9e-28	45.39	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	1.2e-18	36.62	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]

BLAST of CmaCh11G011710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	8.8e-20	36.62	pectin methylesterase inhibitor 1	[more]
AT3G36659.1	1.6e-05	29.58	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..155
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 4..151
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 4..151
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 5..147 e-value: 7.0E-27 score: 105.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 6..151 e-value: 1.4E-28 score: 97.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 11..147 e-value: 5.5E-22 score: 78.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 4..151 e-value: 2.5E-36 score: 126.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 11..152
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 11..151 e-value: 2.07763E-45 score: 143.717

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G011710.1	CmaCh11G011710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity