CmaCh11G011320 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh11G011320 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.9e-52
Identity = 116/287 (40.42%), Postives = 171/287 (59.58%), Query Frame = 0

Query: 176 IGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRASKVYQKPYP 235
           I  AC      L EYM+Y   S C  D ++ +KL+L GC PLPRRRC +R         P
Sbjct: 105 IASACHNYPDLLHEYMNYTPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRT--------P 164

Query: 236 VNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKVKWVRNSSVAV 295
            N S   +  + NV WS Y C++F CL +K    G++       +EK K ++    S  +
Sbjct: 165 RNPS--DSKPESNVLWSYYSCKSFDCLITKFSDLGFD-----LSLEKSKSQFSAYKS-EL 224

Query: 296 DFRISDVLGMKAGE---IRVGLDFGISTGSFGARMREENVTIVTTALNLGAPFNEFIALR 355
           D  IS +L +       +R+G+D G  TGSF A M+  NVT++TT +N  AP++E +A+R
Sbjct: 225 DLPISQLLQIAKSANSVLRLGIDVGGGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMR 284

Query: 356 GLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPGGLLWIDRF 415
           GL+PL+V L QRLP+FD  +DL+     ++ W+ + +++F  FD DR+LR GG LW+DRF
Sbjct: 285 GLVPLHVPLQQRLPVFDGVVDLVRCGRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRF 344

Query: 416 FCDRKDLDD-YIYMFLQFRYKKHKWAIAPKSKN---EVYLSAVLEKP 456
           F  + DL++ Y  M  +  YKK KWA+A K+ +   EV+L+A+L+KP
Sbjct: 345 FSKKVDLENVYAPMIGKLGYKKVKWAVANKADSKHGEVFLTALLQKP 375

BLAST of CmaCh11G011320 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 678.7 bits (1750), Expect = 3.2e-195
Identity = 322/459 (70.15%), Postives = 384/459 (83.66%), Query Frame = 0

Query: 1   MGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTKQIYEASFDAKALSD 60
           M  LQ++LG LVIT++   L++F+S G+    ED C   Y   T K++    FD KAL D
Sbjct: 15  MAGLQVILGLLVITVSLSCLYRFHSVGYFLQNEDTCNNIY---TIKEVSSEGFDLKALHD 74

Query: 61  RVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEMEVIKPLYNAHIGLR 120
           RV+EVL+++++L +KLE  V++ME+ K         + E KK+LE EV+KP Y AHIGLR
Sbjct: 75  RVDEVLEKMDNLYEKLEKTVKDMEKSKDG------SKKEMKKFLEDEVMKPFYYAHIGLR 134

Query: 121 QIRLPKVEKMNENSSMKEEPLINNFVVEEIRKYITPKKSRTGKVNMYMTESIYNTIGHAC 180
           QIRLPK E +  NS+ KEEPLIN F++EEIR+YITPK++R GK+NM+ TE +YNTIGHAC
Sbjct: 135 QIRLPKPEGI-RNSTEKEEPLINKFLIEEIRQYITPKENRVGKINMFGTERVYNTIGHAC 194

Query: 181 VLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRASKVYQKPYPVNESL 240
            L++ ELE+YM YD+G+YC DDWN+ QKLMLNGCDPLPRRRCLTRAS  YQKPYP+NESL
Sbjct: 195 ALMKMELEKYMDYDVGAYCDDDWNLAQKLMLNGCDPLPRRRCLTRASMTYQKPYPINESL 254

Query: 241 WKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKVKWVRNSSVAVDFRIS 300
           WK PDDRNVRW NYQCRNF+CLSSKNPKRGY KCSGCFEMEKEK KWV+NSS+ VDF I 
Sbjct: 255 WKLPDDRNVRWGNYQCRNFACLSSKNPKRGYTKCSGCFEMEKEKDKWVKNSSLLVDFMIE 314

Query: 301 DVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAPFNEFIALRGLIPLYVT 360
           DVL +K  EIR+GLD+G+ TG+F ARMRE+NVTIVTTALNLGAPFNE IALRGLIPLY++
Sbjct: 315 DVLRVKPSEIRIGLDYGVGTGTFAARMREKNVTIVTTALNLGAPFNEMIALRGLIPLYIS 374

Query: 361 LNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLD 420
           LNQRLP FDNTMD+IHTTG MDGW+D+LL+DF+L+DWDRVLRPGGLLWIDRFFC +KDLD
Sbjct: 375 LNQRLPFFDNTMDMIHTTGLMDGWIDLLLMDFVLYDWDRVLRPGGLLWIDRFFCKKKDLD 434

Query: 421 DYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 460
           DY+YMFLQFRYKKHKWAI+PKSK+EVYLSA+LEKPPRAI
Sbjct: 435 DYMYMFLQFRYKKHKWAISPKSKDEVYLSALLEKPPRAI 463

BLAST of CmaCh11G011320 vs. TAIR 10
Match: AT4G01240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 6.7e-116
Identity = 204/433 (47.11%), Postives = 281/433 (64.90%), Query Frame = 0

Query: 49  YEASFD------AKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKK 108
           Y  SFD         +   +E  + ++    D L+++         ++S+ +     H  
Sbjct: 234 YHGSFDGDNYNIGSQMRKTIETAIFKIHQEMDDLKALEANSSTPPSSVSSASGSMFRHVA 293

Query: 109 YLEMEVIKPLYNAHIGLRQIRLPKVEKMNENSSMKEEPLI-------NNFVVEEIRKYIT 168
           +L  +V+  + + H+ L            E  S+ + PL         +F+ EEI+KYI 
Sbjct: 294 FL-ADVLSLVQSVHMELPSF---------EERSVADHPLKQQKGDPGEHFMREEIKKYIK 353

Query: 169 PKKSRTGKVNMYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCD 228
            K +R GK N       + +IGHAC  ++K+LEEYM YD+G  C DDW + QKLM++GCD
Sbjct: 354 IKPNRLGKQNFMGANGTFTSIGHACFAMKKDLEEYMDYDVGEICNDDWRLAQKLMVHGCD 413

Query: 229 PLPRRRCLTRASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSK-NPKRGYNKC 288
           PLPRRRC +R  ++Y KP+P+NESLWK PD+RNVRW  Y+C+NF+CL+S    ++G+ KC
Sbjct: 414 PLPRRRCFSRGPQLYHKPFPINESLWKLPDNRNVRWGQYKCKNFACLASNTTARKGFFKC 473

Query: 289 SGCFEM-EKEKVKWVRNSSV------AVDFRISDVLGMKAGEIRVGLDFGISTGSFGARM 348
           + CF +   E  +W+    +        DF I++VL +K GEIR+GLDF I TG+F ARM
Sbjct: 474 TDCFNLTHHESPRWLNRGEIDPETNQTADFSIAEVLEIKPGEIRIGLDFSIGTGTFAARM 533

Query: 349 REENVTIVTTALNLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDM 408
           RE+NVTIV+  +NLGAPFNE IALRGL+PLY+T+NQRLP FD+T+D+IHTT F+DGW+D+
Sbjct: 534 REQNVTIVSATINLGAPFNEMIALRGLVPLYLTVNQRLPFFDSTLDMIHTTRFLDGWIDL 593

Query: 409 LLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAP---KSKN 458
           +LLDF+LFDWDRVLRPGGLLWID FFC ++D+ DY+  F   RY+KHKW + P   K   
Sbjct: 594 ILLDFVLFDWDRVLRPGGLLWIDGFFCLKEDVSDYMEAFKALRYRKHKWVVVPKKDKDDK 653

BLAST of CmaCh11G011320 vs. TAIR 10
Match: AT3G27230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 131/314 (41.72%), Postives = 195/314 (62.10%), Query Frame = 0

Query: 146 VVEEIRKYITPKKSRTGKVNMYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNM 205
           +V E++ ++   +   GK +      + +++GH+C      L +YMSY +   C DDW++
Sbjct: 102 IVPELKLFLQRHQLPLGKDSRTGITEMVSSVGHSCGKSTDLLSQYMSYKVFDRCPDDWSL 161

Query: 206 GQKLMLNGCDPLPRRRCLTRASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSK 265
           GQKL+L  C+PLPRRRCL +  +  Q      +SLW++  +++V WS   C++F CL  K
Sbjct: 162 GQKLILRACEPLPRRRCLAKTVQ-KQDLSKSPDSLWRSVSNKSVNWSGLGCKSFDCLKGK 221

Query: 266 NPKRGYNKCSGCFEMEKEKVKWVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGA 325
              +   +C GCF++  EK ++V+      DF I DVLG+ +G+IR+G D    +G+F A
Sbjct: 222 KLSK---ECVGCFDLGVEKDRFVKVKG-KNDFLIDDVLGLGSGKIRIGFDISGGSGTFAA 281

Query: 326 RMREENVTIVTTALNLGAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWV 385
           RM E+NVT++T  LN GAPF+EFIA RGL PL+++L+ R P  DN  DLIH +  +D   
Sbjct: 282 RMAEKNVTVITNTLNNGAPFSEFIAARGLFPLFLSLDHRFPFLDNVFDLIHASSGLDVEG 341

Query: 386 DMLLLDFILFDWDRVLRPGGLLWIDRFFC-DRKDLDDYIYMFLQFRYKKHKWAIAPKSKN 445
               L+F++FD DRVL+P GL W+D F+C + +   +   M  +F YKK KW I  K+  
Sbjct: 342 KAEKLEFLMFDLDRVLKPRGLFWLDNFYCANDEKKKELTRMIERFGYKKLKWVIGEKADA 401

Query: 446 EVYLSAVLEKPPRA 459
           +VYLSAVL+KP RA
Sbjct: 402 QVYLSAVLQKPVRA 410

BLAST of CmaCh11G011320 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-65
Identity = 135/359 (37.60%), Postives = 212/359 (59.05%), Query Frame = 0

Query: 108 VIKPLYNAHIGLRQIRLPKVEKMNENSSMKEEPLINNFVVEEIRKYITPKKSRTGKVNMY 167
           +++ + ++H  L Q+    +   + + S +E P       +E++ ++   +   GK +  
Sbjct: 71  ILREIDSSHHTLTQMEKQIIGYESLDLSQQEVP-------QELKLFLQQHQLPLGKDSRT 130

Query: 168 MTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRAS 227
               +  ++GH+C +    L +YMSY++   C DDW++ QKL+L  C+PLPRRRCL   +
Sbjct: 131 GITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCL---A 190

Query: 228 KVYQKP----YPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEM--E 287
           K   KP    +P  +SLW+   + +V WS   C++F CL  K   R    C GCF++   
Sbjct: 191 KTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKGKKLSR---DCVGCFDLATS 250

Query: 288 KEKVKWVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNL 347
            EK ++V+ +    DF I DVL +  G+IR+G D    +G+F ARM E+NV I++  LN+
Sbjct: 251 HEKDRFVKVNG-KTDFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNI 310

Query: 348 GAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDML--LLDFILFDWDR 407
            APF+EFIA RG+ PL+++L+QRLP +DN  DLIH +  +D  V      L+F++FD DR
Sbjct: 311 DAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDR 370

Query: 408 VLRPGGLLWIDRFFCDRKDLDDYIYMFLQ-FRYKKHKWAIAPKSKNEVYLSAVLEKPPR 458
           +L+PGGL W+D F+C   +    +   ++ F YKK KW +  K+  EV+LSAVL+KP R
Sbjct: 371 ILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDAEVFLSAVLQKPAR 413

BLAST of CmaCh11G011320 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-65
Identity = 135/359 (37.60%), Postives = 212/359 (59.05%), Query Frame = 0

Query: 108 VIKPLYNAHIGLRQIRLPKVEKMNENSSMKEEPLINNFVVEEIRKYITPKKSRTGKVNMY 167
           +++ + ++H  L Q+    +   + + S +E P       +E++ ++   +   GK +  
Sbjct: 71  ILREIDSSHHTLTQMEKQIIGYESLDLSQQEVP-------QELKLFLQQHQLPLGKDSRT 130

Query: 168 MTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTRAS 227
               +  ++GH+C +    L +YMSY++   C DDW++ QKL+L  C+PLPRRRCL   +
Sbjct: 131 GITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCL---A 190

Query: 228 KVYQKP----YPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEM--E 287
           K   KP    +P  +SLW+   + +V WS   C++F CL  K   R    C GCF++   
Sbjct: 191 KTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKGKKLSR---DCVGCFDLATS 250

Query: 288 KEKVKWVRNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNL 347
            EK ++V+ +    DF I DVL +  G+IR+G D    +G+F ARM E+NV I++  LN+
Sbjct: 251 HEKDRFVKVNG-KTDFLIDDVLDLGDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNI 310

Query: 348 GAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDML--LLDFILFDWDR 407
            APF+EFIA RG+ PL+++L+QRLP +DN  DLIH +  +D  V      L+F++FD DR
Sbjct: 311 DAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDR 370

Query: 408 VLRPGGLLWIDRFFCDRKDLDDYIYMFLQ-FRYKKHKWAIAPKSKNEVYLSAVLEKPPR 458
           +L+PGGL W+D F+C   +    +   ++ F YKK KW +  K+  EV+LSAVL+KP R
Sbjct: 371 ILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEKTDAEVFLSAVLQKPAR 413