CmaCh10G010460 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G010460
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAsparagine--tRNA ligase
LocationCma_Chr10: 5848617 .. 5853271 (-)
RNA-Seq ExpressionCmaCh10G010460
SyntenyCmaCh10G010460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGACTTTAAATGCCAAGTGTGGATTAGGGTTTAGAATTTCACTTCAGCAGTTGAGGTATCCACCGGCGACAGTCCAATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAGGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGTGTTGTTGTCGACGGTGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTTCGGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGGTAAAAGAAATTGAAATTGTCTTCCGAATCTGAATAATTAGAAATTGAATCAGATTATTTCGATTGGGGTTTTCACATTTGTGGGTTTCATTTATTGCATTTTTCTGTAACTGATTATCAGTACACTTTTATCCACGAGTTCATGTTTTCTCTACGATTACAGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCTTATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACCACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCGGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGATTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTATTTACTATGCTTAAATTTTGTTCCTAGTTGTATTCTTTTTTGCGTACTCAATTGGAATTGGGTTCATGTCTCTGGCTTAGAAGATTGACTACGTATAACTATCAAAAGAAACAATACCTCTGACATTTTATCTTGTAGTGGATGCTTTATGTTCTAGGATCAGTTTATCTCTTCCTTTAGTTTTGTAATTAATTTCTATAAGAAAGCCATTTTGGTTGGCCTAGTGGTCATTCGTTAATATAACTAACGATAGGAGGAAATGGGTTCAAGCCATAGTTGCCGTTTATCTAATATTTATCTTCCTATGAGTTTCTTTCGTAACTAAATGTTAATCTTGTGCACAATCTGGCCTTTCCTTAAGAACTCTAATTGAGAAGAAGTTGCTAGAGGGCTTGGTTTAAGTTTGCTGTGTTCTCTTTAACTACTATGCATATGGCAATCAACCATGGAACTGGTTAGGATAGGAATAAGAAAAGCAACGGAACTCTGCAAATAGGGATTTAGTTCTCTTTTGTTGATTGTATGCTCTTCTTCTAGAGATTTTGATTGTATGCTCTTCTTCTAGAGGTTTAATTGAATTAACCTTGTGTGACATCTTTTCTTTAATTGTTTGAAGGATGATATGAATTGTGCGGAGGCATATGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCTTAGATGACATGCAATTTATGGCCGACAAGTATGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTCGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGGTTTGTGAACTCCGATGTTTTTCATTTACATTCCACTTGAAACAATGGTGATTATTTTTCTGCTTCCTTGCTCAGATACTTGACTGAGGTAGTATTTAAAAAGCCCGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTAGTTAAGTTATCTGTGATTTGTTTCACCATTTTTGACCTTTTGTTTGAAAAAAATTGAATTCTTTGCTCTATTGAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGAGACAGGTAGAAGAACAATACATAGTTTTCGGGTTGTTTCTCCTTGGTGAACTTTGAAGTTTCAATTGTTTTTCCCTGCAGAATCAAGGAGGTAGATTTGCCGCTCGAGCCATACGAATGGTACCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTCGGCCTCGGATTCGAGCGTATGCTTCTTTTTGCCACTGGGATTGACAACATCAGAGATGTTATACCATTCCCCAGATACCCAGGACGAGCAGACCTATGATCATATATATTTACACTAAAGAGATATGTTGTTGGGTTTTAATTTATGCTCATAAATTTTGGATTGGTGGGTTTATTTTATACAGAAACTATTGCATTTCAAATGACTTCAATTGGTTTGATGTGTTAGATATTTTGGTACTAACAGATTTTAATTTGTTTACAGAGAAGATTGTAAAAAGAATGTAAAAGTGGATCAATGTCACAACAAGAGTCTATTATGGGAGCGATTACTTCGACATTCATTTCACGTAAGACCCTAATTTTGCTTATGAATTTTTCGTTGAACTTTACTCGAATTTATACCTTTTAGATTCATTCACTTTTAGCATTTTTTTGACTACAACTTCAAATTTTAAGGATTAAAATGAATATTTTTAAAGTTTAGAATATAATTAAAGTATTTTTTTCCCCTTTTAGAGAGTATATAACCCTTTTAATTGATGGAGAAGGATTTAGGACGCTCACCATTACGGTCTCAGTAGGAGGAAAAGAATTGGGTAGGGCCTACGTGATTTGGGGCGAGTATAAACATAATGCCTCTATCAATTTACAAAAAGTTGGTACCTATCACTTTAAGTAGATCATTTTTTAAAGTTGGTGGAACTCTGGTAGATGTATACAAGGGCACAATTATATTCTTAAGCTTGAATGACCAACCAATAGAATTTAACATGAATGAGGGTATGAAGTATCCACAAAGCCCATAGGGGTGATGGAAAGCCGGCACCATGCCTCCTTATAATAGGTTTTAAAATATTTCTAATCATTCTAGATTATACATTATTTTGGCAAGCGATCATACTCCTTGTGGAGCATTCGTCGAATATTTGATTGACACCAAATTTCGTAAGCTTTCATTTTTCATATAGACGGACATAATTCAAGATTTGCATCGTTTTCTCCAACCTTGCTTATGTCTCAGCTTTGTTTTAATGCACTCTTCTTGGTGTGTGGATGATGCATTGTCCTTCTCGATCACATTTATGTCACTTGTCTTGTTTTTGTCCTTACATGGTTAGATAGGTGCCATTTGAGAGTTCACACCTTGTGTGTTGCATGTGGGGGTCACAACTTACGGTCTTCATAGTGTTGTTGACTTAAACTAGTCATCTTCCTCGATGACTTATTCAAGGGCACAATCTTATCGTGGATTATGACTCTCCTCCACTTAATCTCAATATCGTCGATCCAAATATAGGATTGGTTGCTAACTTGATTCGCTGAACTTTATCGCGCCTCAGAATAAATACGAAAACTTGTGCACTATTTATGGGCTATCCCATTATCGAACCTTATCTCGGTCTCCGAGATTTCCAACATAAACACAACAAAACACAACTGCCTAATGGTTATGGGCTATCCCACTGGGAGTTAACTCGCCCACTTAGAATCGATATGGGACAAACCATGACAAAACTAGCATCGGCATTCCACGCCTCGGCTAGGGGAGTGTGCCCCAAACGTGGCCAAACTTTGGCCTCAACCATCATATTATTTGAGCCGTACAAAGATATGTATGACGACATTTCCTAGTTAACGCCACATTCCTTTATCGTTGCAAAGTTGGTTTGTGTATCATGATATTTTCTTGTCATGATCACATCTCTTTTTCAGAAGCAAGGTCTGGAAGTTTATAACATAACTCTGTATTGGCACTCCACTCCAACCAACTTCTCATTTGCCCACCTTTTGTGAAAAACGTTCTCTTTCTCGAGCAAGTGCTGGTCTTGACACTCTTATGAAGCCACCCACTTTTTATGAAGCCACCCACTTTACTGGAAATACTCAAGTCTCGTCTTGACACTCTTATGAAGCCACCCACTTTACTGGAAATACTCAAGTC

mRNA sequence

GGAGACTTTAAATGCCAAGTGTGGATTAGGGTTTAGAATTTCACTTCAGCAGTTGAGGTATCCACCGGCGACAGTCCAATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAGGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGTGTTGTTGTCGACGGTGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTTCGGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCTTATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACCACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCGGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGATTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGTGCGGAGGCATATGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCTTAGATGACATGCAATTTATGGCCGACAAGTATGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTCGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTAGTATTTAAAAAGCCCGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGAGACAGAATCAAGGAGGTAGATTTGCCGCTCGAGCCATACGAATGGTACCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTCGGCCTCGGATTCGAGCGTATGCTTCTTTTTGCCACTGGGATTGACAACATCAGAGATGTTATACCATTCCCCAGATACCCAGGACGAGCAGACCTATGATCATATATATTTACACTAAAGAGATATGTTGTTGGGTTTTAATTTATGCTCATAAATTTTGGATTGGTGGGTTTATTTTATACAGAAACTATTGCATTTCAAATGACTTCAATTGGTTTGATGTGTTAGATATTTTGGTACTAACAGATTTTAATTTGTTTACAGAGAAGATTGTAAAAAGAATGTAAAAGTGGATCAATGTCACAACAAGAGTCTATTATGGGAGCGATTACTTCGACATTCATTTCACAAGCAAGGTCTGGAAGTTTATAACATAACTCTGTATTGGCACTCCACTCCAACCAACTTCTCATTTGCCCACCTTTTGTGAAAAACGTTCTCTTTCTCGAGCAAGTGCTGGTCTTGACACTCTTATGAAGCCACCCACTTTTTATGAAGCCACCCACTTTACTGGAAATACTCAAGTCTCGTCTTGACACTCTTATGAAGCCACCCACTTTACTGGAAATACTCAAGTC

Coding sequence (CDS)

ATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAGGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGTGTTGTTGTCGACGGTGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTTCGGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCTTATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACCACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCGGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGATTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCGACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGTGCGGAGGCATATGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCTTAGATGACATGCAATTTATGGCCGACAAGTATGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTCGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTAGTATTTAAAAAGCCCGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCGAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGAGACAGAATCAAGGAGGTAGATTTGCCGCTCGAGCCATACGAATGGTACCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTCGGCCTCGGATTCGAGCGTATGCTTCTTTTTGCCACTGGGATTGACAACATCAGAGATGTTATACCATTCCCCAGATACCCAGGACGAGCAGACCTATGA

Protein sequence

MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Homology
[Close]
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 6.2e-249
Identity = 418/574 (72.82%), Postives = 495/574 (86.24%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D  T    +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ SL D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG+VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  + ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 1.1e-242
Identity = 417/576 (72.40%), Postives = 486/576 (84.38%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D  T    KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++SL DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG+VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK +++ +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-VFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK VF+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.6e-143
Identity = 272/541 (50.28%), Postives = 340/541 (62.85%), Query Frame = 0 

Query: 34  RVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS 93
           R  I+S+         L G  + + GWV+T R  ++  F F+E+NDGS   N Q+I    
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPD 61

Query: 94  LADLGQIV---PTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGSVDPARYPLPKTKLT 153
           +A   Q++    TG  V   G   +  PGK Q +E++A A+  +G  DP  Y L K + T
Sbjct: 62  IAGYDQLINQLSTGVSVSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHT 121

Query: 154 LEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMF 213
            EFLR   HLRPRTNTI AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MF
Sbjct: 122 FEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMF 181

Query: 214 QVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVME 273
           QVTT           L +N P    E                                  
Sbjct: 182 QVTT-----------LDQNNPARTPE---------------------------------- 241

Query: 274 LKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVY 333
                                    G+VDY +DFF +  +LTVSGQL  E YACALS VY
Sbjct: 242 -------------------------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVY 301

Query: 334 TFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMQF 393
           TFGPTFRAENS+TSRHLAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F
Sbjct: 302 TFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEF 361

Query: 394 MADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERY 453
                    I RL+ V +T F R SYT AV ILE+A KK   FE  V+WG+DL SEHER+
Sbjct: 362 FNKHVATDLISRLEHVINTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERF 421

Query: 454 LTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIR 513
           L E  F KPVI+ +YPK IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+ 
Sbjct: 422 LAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLE 466

Query: 514 DRIKEVDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRA 572
            ++KE +LP E Y WYL+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+A
Sbjct: 482 SKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKA 466

[Close]
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.4e-139
Identity = 257/525 (48.95%), Postives = 328/525 (62.48%), Query Frame = 0 

Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G+ DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 VKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAK 288
             NP                                             +++ + EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G  FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  E+ +P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-139
Identity = 256/525 (48.76%), Postives = 327/525 (62.29%), Query Frame = 0 

Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G+ DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 VKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAK 288
             NP                                             +++ + EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G  FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  E+ +P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 571/572 (99.83%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match: A0A6J1DMU4 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 562/572 (98.25%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAE+SAPPADQLAA TLNDDAPTY +LKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAENSAPPADQLAAATLNDDAPTYKALKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QKVELRA+AVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 QQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAE+LEKEL+KNPPPSEA+V+ A
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAEKLEKELIKNPPPSEADVEAA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGE VAKLKA+KASKEEIGASV+ELKKAKENV++LEER+KLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEDVAKLKAAKASKEEIGASVLELKKAKENVARLEERSKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEV FKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVEFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGEL+GGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELVGGSQREERYEVIRDRIKEMDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match: A0A6J1HLL2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 561/572 (98.08%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AVTLNDDA TY SLKAEFSDRVPIR+IV RPDGG+GLAGQKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKASKASK+EIGASV+ELK+AKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKEV LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match: A0A5D3CE86 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003180 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 557/572 (97.38%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAVTLN+DA  Y SLKAEFSDRVPIR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRF+CQWLLDNCLDDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. NCBI nr
Match: XP_022988059.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 571/572 (99.83%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. NCBI nr
Match: XP_023520843.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 570/572 (99.65%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEH APPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHPAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. NCBI nr
Match: KAG6590322.1 (Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 568/572 (99.30%), Postives = 570/572 (99.65%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAP ADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPTADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. NCBI nr
Match: XP_038879816.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 547/572 (95.63%), Postives = 562/572 (98.25%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLN+DAPTY+SLKAEFSDRVPIR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNNDAPTYDSLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADL QIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLSQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKASKASKEEIGASV+ELKKAKENV+KLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVANLKASKASKEEIGASVLELKKAKENVAKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRF+CQWLLDNCLDDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEV+FKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EVLEEAVKKGKEFENKVEWGIDLASEHERYLTEVIFKKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVI DRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIHDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]
BLAST of CmaCh10G010460 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 861.3 bits (2224), Expect = 4.4e-250
Identity = 418/574 (72.82%), Postives = 495/574 (86.24%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D  T    +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ SL D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG+VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  + ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]
BLAST of CmaCh10G010460 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 840.5 bits (2170), Expect = 8.0e-244
Identity = 417/576 (72.40%), Postives = 486/576 (84.38%), Query Frame = 0 

Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D  T    KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++SL DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG+VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK +++ +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-VFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK VF+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]
BLAST of CmaCh10G010460 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 491.9 bits (1265), Expect = 7.0e-139
Identity = 266/553 (48.10%), Postives = 346/553 (62.57%), Query Frame = 0 

Query: 24  YNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    GQ + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEASLADLGQI----VPTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGSVDP 143
            NLQ ++ +      Q+    + TG  V V G   +   G  QKVEL+ + ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI-VASQGTKQKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKA 263
            SDCEGAGE F VTTL+                                          +
Sbjct: 261 ASDCEGAGEQFCVTTLI-----------------------------------------PS 320

Query: 264 SKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQV 323
           S E    S+  + K                    K G +D+++DFF + AFLTVSGQL  
Sbjct: 321 SHENTDTSIDAIPKT-------------------KGGLIDWSQDFFGKPAFLTVSGQLNG 380

Query: 324 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQW 383
           ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA AY++++ ++
Sbjct: 381 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 440

Query: 384 LLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEW 443
           +LDNC +DM+F     ++  I RL  V+   F++  YT+A+EIL +A KK   F+  V+W
Sbjct: 441 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKK---FDFPVKW 500

Query: 444 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 503
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 501 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 560

Query: 504 GSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 563
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 561 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 566

Query: 564 DVIPFPRYPGRAD 572
           DVIPFPR P  A+
Sbjct: 621 DVIPFPRTPASAE 566

[Close]
BLAST of CmaCh10G010460 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 478.4 bits (1230), Expect = 8.0e-135
Identity = 264/634 (41.64%), Postives = 385/634 (60.73%), Query Frame = 0 

Query: 18  NDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDA------ 77
           ND +P   +L +++S RV +++++ R D GAGLAG++V +GGWVK+ R   K++      
Sbjct: 14  NDLSPKPITL-SKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 73

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 74  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 133

Query: 138 -------FAFLELNDGSCPQNLQVIIEASLADL--GQIVPTGTCVVVDGVFKLP--PPGK 197
                  F FL ++DGS   +LQV+++++L+ +   Q++  GTC+V +GV +LP     K
Sbjct: 134 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 193

Query: 198 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 257
           H  +EL A+ ++HVG+VDP +YPL K +L L  LRDF H RPRT T+ +V R+ +AL  A
Sbjct: 194 H-VIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLA 253

Query: 258 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAE---V 317
           +HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +
Sbjct: 254 SHTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSI 313

Query: 318 DEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGK 377
           D  + ++KEK   +  LK S +++E + A+V +LKK  +  S++E + K K G   K  K
Sbjct: 314 DTVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEK 373

Query: 378 VDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEI 437
           +D++KDFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+
Sbjct: 374 LDFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEM 433

Query: 438 AFADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYT 497
           AFA+L D M+CA+ Y +FLC+++L+N  +DM+F++ + D+T   RL+  +S+  +R SYT
Sbjct: 434 AFAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYT 493

Query: 498 EAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLN 557
           E + +L++A      FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLN
Sbjct: 494 EVISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLN 553

Query: 558 DDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKH 572
           DD KTVAA D++VPKVG +I GSQ EER+E++  RI E     E +EWYLDLRR+GTVKH
Sbjct: 554 DDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKH 613

[Close]
BLAST of CmaCh10G010460 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 6.1e-26
Identity = 90/291 (30.93%), Postives = 144/291 (49.48%), Query Frame = 0 

Query: 286 KLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  ++Q +  +Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R ++ E V++L+EA         +V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA-------GVEVDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E  +
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

The following BLAST results are available for this feature:
BLAST of CmaCh10G010460 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SW966.2e-24972.82Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK11.1e-24272.40Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC91.6e-14350.28Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
Q7MVE51.4e-13948.95Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ... [more]
B2RK424.0e-13948.76Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207... [more]
BLAST of CmaCh10G010460 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JKK10.0e+00100.00Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1[more]
A0A6J1HAP90.0e+0099.48Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1[more]
A0A6J1DMU40.0e+0094.76Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1[more]
A0A6J1HLL20.0e+0094.58Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1[more]
A0A5D3CE860.0e+0094.76Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
BLAST of CmaCh10G010460 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022988059.10.0e+00100.00asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima][more]
XP_022961000.10.0e+0099.48asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A... [more]
XP_023520843.10.0e+0099.48asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo][more]
KAG6590322.10.0e+0099.30Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. s... [more]
XP_038879816.10.0e+0095.63asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida][more]
BLAST of CmaCh10G010460 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G56680.14.4e-25072.82Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.18.0e-24472.40Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.17.0e-13948.10Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT3G07420.18.0e-13541.64asparaginyl-tRNA synthetase 2 [more]
AT4G31180.16.1e-2630.93Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 264..284
NoneNo IPR availableGENE3D2.40.50.140coord: 33..138
e-value: 4.8E-10
score: 41.2
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 143..571
e-value: 1.8E-106
score: 358.3
NoneNo IPR availableGENE3D1.10.287.10coord: 230..282
e-value: 5.1E-5
score: 25.1
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 1..572
NoneNo IPR availablePANTHERPTHR22594:SF52BNAC03G13340D PROTEINcoord: 1..572
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 148..568
e-value: 4.21985E-143
score: 414.656
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 54..135
e-value: 9.20831E-26
score: 98.7916
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 151..567
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 327..340
score: 43.83
coord: 487..503
score: 44.79
coord: 311..323
score: 40.73
coord: 527..541
score: 47.27
IPR000738WHEP-TRS domainSMARTSM00991WHEP_TRS_a_2_a_3_acoord: 240..297
e-value: 0.0065
score: 25.3
IPR000738WHEP-TRS domainPROSITEPS51185WHEP_TRS_2coord: 236..292
score: 10.268002
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 49..571
e-value: 7.5E-167
score: 553.7
IPR004522Asparagine-tRNA ligaseHAMAPMF_00534Asn_tRNA_synthcoord: 37..572
score: 42.120655
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 152..214
e-value: 3.4E-12
score: 46.1
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 301..565
e-value: 1.3E-62
score: 211.7
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 329..562
score: 13.63026
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 48..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G010460.1CmaCh10G010460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding