CmaCh10G001270 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G001270
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCASP-like protein
LocationCma_Chr10: 574310 .. 577849 (-)
RNA-Seq ExpressionCmaCh10G001270
SyntenyCmaCh10G001270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTAAATTTTGGAGAGCTTCGAGCTTCGAGCTTCGAGCTTCGAGCTTCGACTACTCCCTCAGGATGTTGAAATTACGTTCGTAAAATAATTAGTCGAGAAATTCATCTTCTAGGTTAAACTTGTCAGGTGGAGCCCGTCATTAAACTAATAACAATTAAAAACTATTTAATAATTTTCTTTTAATTGAGATATTATTTAGAGTAATTAAGGGAATATGTTATTACCTTCGAAGTTGAAAGAACAATTACGTGGCAAAAAACGACGTCGTAAAGAGTGGGAGTAAACAGAGCTCAATTTGACGATGAATCTTGAGTGAGTAGTAGTCATCTTTGACAGTGAAGGTTTTAAATGATGGAATATGTATTTAAATAAAAAAAAAAAGGAATCGTAGGCTTCGCAGCTCACTTTACTTTGTCGGAGAGGCTCTAAAATACGGTAGAATCCACTGCTATGAACGAATCAAATTCTTCCACGTCACACAAAACCTTACGTGTAACCAGACTCTTTTTTTCTATTATTCCCATTCTGAACGTAAGATTAGCTCGCCTTCTTCAGAACTTATGTACACTGTACCGCACTGGAATATATTCTCGCCGTTACTTTAAACCTACAAATAAAATGATGCCACGTGGCTTCCAAATTACGAACGCGAACTTAGAGACGAGTCCCCCTAAAGTCCTCTGCTTTCTCTACCTTTTTTTTCCCGACTTCCCAAACAGCCTTTTCCTTTTTATTTTATTTTATTTATTTCTTTTCGTTCTTTGTGAACTAATTTTTCCCGCAAATCTTTCAATAAAATCCTTCGAGGTTGGAAGAAAAATGATTGAGGCTAAATTGAAATCGAACGACTCGTCATCCATGAGGAAGTCGTCGTCGAGAAATTCCGAGTTATCGGTTCACAGTCAGATGGAATCTCCACACTCACCGCTGCGCTTCCACTCGCCGCTCCGATTCGACGTCGGCGACCTTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCAGACAGATAACTCCAAGGCGATTGTCGCCGTTGATGTTGTCGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCAGCTCCCGGAAGACGCCGGAGAATGTGAGCTTTCCCGGAGATAGGTCGACTTCGTCTAAGGTTATGTTTAATAGGGCAATGAAGGAGGATGTGCCGCAGTCGGTGACCAAAGTAGGGCCGGCTGCAGGTGGAATCGGGGAGCGCGGTGGTGGAGGTGAGAGATCGCCGAAGCAAATGCCGCCGATGTCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGTGGCGACTCCTTTGATCGTTACAAGGAATACAGGTTTTCTTCTTACGATTCTTGACTCCTCTTTACTATCTGAGTGCTATTCAACTACAGAATGAAGTAAATTAGATTTGCTTTTCGATGTTTTGTTATTTTCTTTCCGAATTGTTGTTCAATTCCTTAGGCTTAATCTTTGCAGACTTGAGTATTTTAATCTTTAACTAGTAGTATGATCTGTGGGACCTCAAGATTAATACCATACCAATTAGCTTTCCATTCTTCGGATTCTATAAATTTCCTTCCAACCAGAATCAAAACTAGAAACAATTATTGTGATTAATGGTTTTGAGATTCTTCTGTTGGTTGTACACCATATTATCCTCAAATGAGTTTGTGTCTTAGTTGCAGTCTTCAATCTCAATCTTTAATGCCATGTTAAAATGGAGCTGTCACCTTCTTCCAACCTATTGATTGTCAAGTTCTCAATGATATACACAATCCAAATGAATTCGTTCGCTACACTATTTAATTCAGTCTGACCCACAACTTTGTATTACAACTTTTATGTCCTTCGCCGAGTTTTTTGCTGCTTTGAGTTTAAGTTTTTAGTTCTCATATTGGTTTTTGGTATAATTTCGAGTGTTTGTACTGTGAGTGAAGGATTGCGGTGGAAGCAATCTCACTATCAGAGCTGTCTCTTAATTTCCATGCCTTTTTGTAATCAATCATGTGGCATTATTCTTAGCTTCCCTTGTATTGCAAGAATACATCAATATGTCATGCTATAACGTGAAAACCGTACTTAAAGGATGTATCTCATATTCTTTTTGTTCAAATTATTCTAGAAGTCTGTATGTTTGTTCCCGTCTTATATTATCTCATCAAGTCGATTTATCAACAGGTACTGCCTCACCGTGAATATAATTGCGTTCGTGTATGCAGTGTTTCAAGCATTTGATCTTGTCTATACTCTATTCAAGAAGAACCATATGATACGCCATCACTTTCGTTATCATTTTGACTTCTTCATGGATCAGGTTAGTTGTGCAAATAAGCGGGTCTGAATGAATATTATTTAATATCTATAGGCATGCTTTCACTGTTAGTCTGCTACAATGAATGTGTCTCTATGGTTCAAGGCTTGTTTAGTTTACTTAGGCTAGAGGAAATTCGCTGAATTGCATGGAGAATCTTTTTTCTGCTTCACCGCTTGAATACGGTTTACTCAATCTGAGACGATCATTCAAGTGGGGATGAACTTGATAGTTGATCTGCCTGCTGATCACTTGTCTCGTATAATGATTGATTGTGTCATTGTGTGAGTGTAGATTGTTCAGGCTTCTGATTTGCTCAAATATTCAAGGGCTTGTTTGGATTGGGTTGACTTTCTAAGTGTTGTTTGTAAGTGAAAAAATAGTGGTTTTAAGCTTTGAAATATCATATCGGTTGGAGAGGGAAATGAAGCATTCCTTATAAAAGTGTGGAAACCTCTCTCTAGTCGACACGTTTTAAAATCTTGAGGGGAAGTCCGGAGGGGAAAGTCCAAAGAAGACAATATGTGCTAGTAGTAGATTTGGGCTGTTACACCTTGAAAGTGAATCCAAACAAAACCTATTGTGGTTAATCATCAAGTCTTCTGCCTCTCGAGTCTCAGCGAGAACTTTCTTGATAACCCAAGATTGTTAGCTATTCTGCCTGCTTCGTTTCTTCATCAACTAATGATGTAATCCTGGATTATATTGGCAGGCATTGGCATATCTTCTGATATCGTCGTCTTCCTCGGCCGCAACACGTGTTGATGACTGGCAATCAAATTGGGGAAAAGATGAATTCACGCAGATGGCCAGTGCATCGGTCAGCATGTCTTTCCTTGCCTTTGTTGCTTTTGCTATTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCATGCTTGATATCAAAATCTCAGACCTCTTCGTTTTTCCTTGAAAAGAGAACAATTTTTACATTGATTGTCAACCCATTTTTTTTCTCTTGATGTTGTACAGAGATATCATAGCTAGCTTTGACCTTACAATAAAGAGGATACCAAATTGTATAATTGTTGGATTGTGTGTATTGCATATTTCATATATATATATATAAATGAAGCTTCTATTGTTGTAGCAGCAAA

mRNA sequence

TTTTAAATTTTGGAGAGCTTCGAGCTTCGAGCTTCGAGCTTCGAGCTTCGACTACTCCCTCAGGATGTTGAAATTACGTTCGTAAAATAATTAGTCGAGAAATTCATCTTCTAGGTTAAACTTGTCAGGTGGAGCCCGTCATTAAACTAATAACAATTAAAAACTATTTAATAATTTTCTTTTAATTGAGATATTATTTAGAGTAATTAAGGGAATATGTTATTACCTTCGAAGTTGAAAGAACAATTACGTGGCAAAAAACGACGTCGTAAAGAGTGGGAGTAAACAGAGCTCAATTTGACGATGAATCTTGAGTGAGTAGTAGTCATCTTTGACAGTGAAGGTTTTAAATGATGGAATATGTATTTAAATAAAAAAAAAAAGGAATCGTAGGCTTCGCAGCTCACTTTACTTTGTCGGAGAGGCTCTAAAATACGGTAGAATCCACTGCTATGAACGAATCAAATTCTTCCACGTCACACAAAACCTTACGTGTAACCAGACTCTTTTTTTCTATTATTCCCATTCTGAACGTAAGATTAGCTCGCCTTCTTCAGAACTTATGTACACTGTACCGCACTGGAATATATTCTCGCCGTTACTTTAAACCTACAAATAAAATGATGCCACGTGGCTTCCAAATTACGAACGCGAACTTAGAGACGAGTCCCCCTAAAGTCCTCTGCTTTCTCTACCTTTTTTTTCCCGACTTCCCAAACAGCCTTTTCCTTTTTATTTTATTTTATTTATTTCTTTTCGTTCTTTGTGAACTAATTTTTCCCGCAAATCTTTCAATAAAATCCTTCGAGGTTGGAAGAAAAATGATTGAGGCTAAATTGAAATCGAACGACTCGTCATCCATGAGGAAGTCGTCGTCGAGAAATTCCGAGTTATCGGTTCACAGTCAGATGGAATCTCCACACTCACCGCTGCGCTTCCACTCGCCGCTCCGATTCGACGTCGGCGACCTTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCAGACAGATAACTCCAAGGCGATTGTCGCCGTTGATGTTGTCGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCAGCTCCCGGAAGACGCCGGAGAATGTGAGCTTTCCCGGAGATAGGTCGACTTCGTCTAAGGTTATGTTTAATAGGGCAATGAAGGAGGATGTGCCGCAGTCGGTGACCAAAGTAGGGCCGGCTGCAGGTGGAATCGGGGAGCGCGGTGGTGGAGGTGAGAGATCGCCGAAGCAAATGCCGCCGATGTCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGTGGCGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACCGTGAATATAATTGCGTTCGTGTATGCAGTGTTTCAAGCATTTGATCTTGTCTATACTCTATTCAAGAAGAACCATATGATACGCCATCACTTTCGTTATCATTTTGACTTCTTCATGGATCAGGCATTGGCATATCTTCTGATATCGTCGTCTTCCTCGGCCGCAACACGTGTTGATGACTGGCAATCAAATTGGGGAAAAGATGAATTCACGCAGATGGCCAGTGCATCGGTCAGCATGTCTTTCCTTGCCTTTGTTGCTTTTGCTATTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCATGCTTGATATCAAAATCTCAGACCTCTTCGTTTTTCCTTGAAAAGAGAACAATTTTTACATTGATTGTCAACCCATTTTTTTTCTCTTGATGTTGTACAGAGATATCATAGCTAGCTTTGACCTTACAATAAAGAGGATACCAAATTGTATAATTGTTGGATTGTGTGTATTGCATATTTCATATATATATATATAAATGAAGCTTCTATTGTTGTAGCAGCAAA

Coding sequence (CDS)

ATGAACGAATCAAATTCTTCCACGTCACACAAAACCTTACGTGTAACCAGACTCTTTTTTTCTATTATTCCCATTCTGAACGTAAGATTAGCTCGCCTTCTTCAGAACTTATGTACACTGTACCGCACTGGAATATATTCTCGCCGTTACTTTAAACCTACAAATAAAATGATGCCACGTGGCTTCCAAATTACGAACGCGAACTTAGAGACGAGTCCCCCTAAAGTCCTCTGCTTTCTCTACCTTTTTTTTCCCGACTTCCCAAACAGCCTTTTCCTTTTTATTTTATTTTATTTATTTCTTTTCGTTCTTTGTGAACTAATTTTTCCCGCAAATCTTTCAATAAAATCCTTCGAGGTTGGAAGAAAAATGATTGAGGCTAAATTGAAATCGAACGACTCGTCATCCATGAGGAAGTCGTCGTCGAGAAATTCCGAGTTATCGGTTCACAGTCAGATGGAATCTCCACACTCACCGCTGCGCTTCCACTCGCCGCTCCGATTCGACGTCGGCGACCTTCCGGAGACCCCTCCTTATGCCTCTCCCGATACCTCCCCTGAGAAGCAGACAGATAACTCCAAGGCGATTGTCGCCGTTGATGTTGTCGATAAGAGTACTCAGTTCTCGCCGCTTCCGTCTCCCTACGCCAGCTCCCGGAAGACGCCGGAGAATGTGAGCTTTCCCGGAGATAGGTCGACTTCGTCTAAGGTTATGTTTAATAGGGCAATGAAGGAGGATGTGCCGCAGTCGGTGACCAAAGTAGGGCCGGCTGCAGGTGGAATCGGGGAGCGCGGTGGTGGAGGTGAGAGATCGCCGAAGCAAATGCCGCCGATGTCGAGGAGGTCGAAGGACGATGCGTTGACGAAGGCGGCTTTAGGTTTTAGAGTGTGTGAAGTGGCGGTTTGCTTGATTTCGTTTTCGGTTATGGCGTCTGATAAGACTCAGGGTTGGAGTGGCGACTCCTTTGATCGTTACAAGGAATACAGGTACTGCCTCACCGTGAATATAATTGCGTTCGTGTATGCAGTGTTTCAAGCATTTGATCTTGTCTATACTCTATTCAAGAAGAACCATATGATACGCCATCACTTTCGTTATCATTTTGACTTCTTCATGGATCAGGCATTGGCATATCTTCTGATATCGTCGTCTTCCTCGGCCGCAACACGTGTTGATGACTGGCAATCAAATTGGGGAAAAGATGAATTCACGCAGATGGCCAGTGCATCGGTCAGCATGTCTTTCCTTGCCTTTGTTGCTTTTGCTATTAGTTCGCTTATCTCTGGTTACAACCTTTGTACCCGTGATCATGCTTGA

Protein sequence

MNESNSSTSHKTLRVTRLFFSIIPILNVRLARLLQNLCTLYRTGIYSRRYFKPTNKMMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIKSFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAISSLISGYNLCTRDHA
Homology
BLAST of CmaCh10G001270 vs. ExPASy Swiss-Prot
Match: Q501G6 (CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 8.2e-68
Identity = 164/310 (52.90%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 137 MRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPDTSPEKQTDNSKAI 196
           M++++S NSE    S  ESPHSPLRFHSPL  D GDLPE+  Y SP+ SP K  +N K+I
Sbjct: 3   MKRTASSNSE--AQSYNESPHSPLRFHSPLS-DAGDLPES-RYVSPEGSPFK-IENPKSI 62

Query: 197 VAVDVVDKSTQFSPLPSPY------------ASSRKTPENVSFPGDRSTSSKVMFNRAMK 256
           VA    +K TQFSPLPSP               + + P N S   D+S SS V+ N  ++
Sbjct: 63  VA---GNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSS--SDKSPSSMVVHNSWVR 122

Query: 257 EDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCLI 316
           ED  Q+ T+   A          GE S +    ++R   DD ++  ALGFR+ EV +C+I
Sbjct: 123 EDGGQTTTRKAGAP-------INGEESTR--TTVNRARGDDLVSLTALGFRITEVILCVI 182

Query: 317 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHHF 376
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VN+IAFVY+ F+A D    + K+++MI   F
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGF 242

Query: 377 RYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAIS 435
              F F MDQ LAYLL+S+SS AATRVDDW SNWGKDEFTQMA+AS+++SFLAF AFA+S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

BLAST of CmaCh10G001270 vs. ExPASy Swiss-Prot
Match: D7MMW4 (CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_919556 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.4e-67
Identity = 163/310 (52.58%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 137 MRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPDTSPEKQTDNSKAI 196
           M+++ S NSE   +S  ESPHSPLRFHSPL  D GDLPE+  Y SP+ SP K  +N K+I
Sbjct: 3   MKRTVSSNSE--AYSYNESPHSPLRFHSPLS-DAGDLPES-RYVSPEGSPFK-IENPKSI 62

Query: 197 VAVDVVDKSTQFSPLPSPY------------ASSRKTPENVSFPGDRSTSSKVMFNRAMK 256
           VA    +K TQFSPLPSP               + + P N S   D+S SS V+ N  ++
Sbjct: 63  VA---GNKLTQFSPLPSPIPPPPPQIPPPRRQRNARVPMNSSL--DKSPSSMVVQNSWVR 122

Query: 257 EDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCLI 316
           ED  Q+ T+   A          GE S      ++R  +DD ++  ALGFR+ EV +C+I
Sbjct: 123 EDGQQNTTRKAGAP-------MNGEES--ATTAVNRARRDDLVSVTALGFRITEVILCVI 182

Query: 317 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHHF 376
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VN+IAFVY+ F+A D    + K+++M+   F
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYMAKESYMMNCGF 242

Query: 377 RYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAIS 435
              F F MDQ LAYLL+S+SS AATRVDDW SNWGKDEFTQMA+AS+++SFLAF AFA+S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

BLAST of CmaCh10G001270 vs. ExPASy Swiss-Prot
Match: Q84WP5 (CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.6e-58
Identity = 137/274 (50.00%), Postives = 179/274 (65.33%), Query Frame = 0

Query: 175 ETPPYASPDT-SPEKQ---TDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGD 234
           +T P  SP + S EK    +D S AIVA D  + +T FS  PSP  S   + E+      
Sbjct: 6   KTMPSMSPSSVSTEKSPPPSDTSMAIVAFD--NSTTHFSSSPSPPHSLDHSSESEKEDAK 65

Query: 235 RSTSSKVMFNRAMKEDVP----------QSVTKVGPA--AGGIGERGGGGERSPKQMPPM 294
               S+   N    E+ P          +SV +V P   +  +G     G+RS      +
Sbjct: 66  SKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARVGSGRSSGQRSGAVSAIL 125

Query: 295 SRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIA 354
            R  +++ +  +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++A
Sbjct: 126 RRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVA 185

Query: 355 FVYAVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNW 414
           FVY+ FQA DL Y L K+ H+I HH R  F+F +DQ LAYLL+S+S++A TRVDDW SNW
Sbjct: 186 FVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWVSNW 245

Query: 415 GKDEFTQMASASVSMSFLAFVAFAISSLISGYNL 433
           GKDEFT+MASAS++MSFLAF+AFA SSLISGYNL
Sbjct: 246 GKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of CmaCh10G001270 vs. ExPASy Swiss-Prot
Match: D7LIR2 (CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_482607 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.7e-57
Identity = 132/279 (47.31%), Postives = 176/279 (63.08%), Query Frame = 0

Query: 176 TPPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSP-----YASSRKTPENVSFPGD 235
           +P   S + SP   +D S AIVA D  + +T  S  PSP     ++S  +  +    P  
Sbjct: 5   SPSSISTEKSP-PPSDTSMAIVAFD--NSTTHLSSSPSPPHSLDHSSDSEKEDEKRRPES 64

Query: 236 RSTSSKVMF-------------NRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPP 295
           R   + V               NR++KE VP   T        +G     G+RS   +  
Sbjct: 65  RRNKNPVKIEETPSPIVVVHNHNRSVKEVVPTRKT------ARVGSGRSSGQRSGAVLAI 124

Query: 296 MSRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNII 355
           + R  +++ +   ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++
Sbjct: 125 LRRSRREEIVKFVALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVV 184

Query: 356 AFVYAVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSN 415
           AF+YA FQA DL Y L K+ H+I HH R  F+F +DQ LAYLL+ +S++A TRVDDW SN
Sbjct: 185 AFIYASFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMCASTAAVTRVDDWVSN 244

Query: 416 WGKDEFTQMASASVSMSFLAFVAFAISSLISGYNLCTRD 437
           WGKD+FT+MASAS++MSFL F+AFA SSLISGYNL  +D
Sbjct: 245 WGKDDFTEMASASIAMSFLTFLAFAFSSLISGYNLFNQD 274

BLAST of CmaCh10G001270 vs. ExPASy Swiss-Prot
Match: Q9FNE8 (CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-37
Identity = 106/272 (38.97%), Postives = 146/272 (53.68%), Query Frame = 0

Query: 172 DLPETPPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDR 231
           +L +T  +       +++ D S  I   ++  +S+  S   +   S   +P   S P   
Sbjct: 3   ELEKTQKFQKKKKQQQEKQDQSSPI-NFEMSSRSSLHSLPQTTIESPPDSPTLSSIPDSH 62

Query: 232 STSSKVMF--NRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPP-------MSRRS 291
            +S   +       K + P  VT         GE       S +Q+ P         R S
Sbjct: 63  GSSPHTIIPTPSVAKTETPFRVTN--------GEEEKKVSESRRQLRPSFSSSSSTPRES 122

Query: 292 K-DDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVY 351
           K    + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  N+I FVY
Sbjct: 123 KWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVY 182

Query: 352 AVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKD 411
           + F   DLVY L       RH+ R+  +F +DQ LAYLL S+S+SA+ RVDDWQSNWG D
Sbjct: 183 SGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGAD 242

Query: 412 EFTQMASASVSMSFLAFVAFAISSLISGYNLC 434
           +F  +A ASV++S+++FVAFA  SL SGY LC
Sbjct: 243 KFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of CmaCh10G001270 vs. ExPASy TrEMBL
Match: A0A6J1JCW1 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485636 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 4.5e-210
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 57  MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK 116
           MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK
Sbjct: 1   MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK 60

Query: 117 SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET 176
           SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET
Sbjct: 61  SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET 120

Query: 177 PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK 236
           PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK
Sbjct: 121 PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK 180

Query: 237 VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV 296
           VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV
Sbjct: 181 VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV 240

Query: 297 CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK 356
           CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK
Sbjct: 241 CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK 300

Query: 357 NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL 416
           NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL
Sbjct: 301 NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL 360

Query: 417 AFVAFAISSLISGYNLCTRDHA 439
           AFVAFAISSLISGYNLCTRDHA
Sbjct: 361 AFVAFAISSLISGYNLCTRDHA 382

BLAST of CmaCh10G001270 vs. ExPASy TrEMBL
Match: A0A6J1E1W9 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.4e-166
Identity = 308/315 (97.78%), Postives = 309/315 (98.10%), Query Frame = 0

Query: 124 MIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPD 183
           MIEAKLKS DSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLR DVGDLPETPPYASPD
Sbjct: 1   MIEAKLKSIDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRSDVGDLPETPPYASPD 60

Query: 184 TSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM 243
           TSPEKQ DNSKAIV VDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM
Sbjct: 61  TSPEKQPDNSKAIVLVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM 120

Query: 244 KEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 303
           KEDVPQSVTKVGPAAGG+ ERGGGG RSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL
Sbjct: 121 KEDVPQSVTKVGPAAGGVEERGGGGGRSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 180

Query: 304 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH 363
           ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH
Sbjct: 181 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH 240

Query: 364 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 423
           FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI
Sbjct: 241 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 300

Query: 424 SSLISGYNLCTRDHA 439
           SSLISGYNLCTRDHA
Sbjct: 301 SSLISGYNLCTRDHA 315

BLAST of CmaCh10G001270 vs. ExPASy TrEMBL
Match: A0A6J1C1D1 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 4.3e-144
Identity = 278/321 (86.60%), Postives = 288/321 (89.72%), Query Frame = 0

Query: 124 MIEAKLK---SNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYA 183
           MIE KLK   SNDSSSMRKSSSRNSE S+HS MESPHSPLRFHSPLR D GD PETPPYA
Sbjct: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 184 SPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTP-ENVSFPGDRSTSSKVMF 243
           SPDTSPEK  DNSKAIV VDVVDKSTQFSPLPSPYA SRK P EN +FP DRSTSSKVMF
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 244 NRAMKEDVPQSVTKVGPAA-GGI-GERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVC 303
           NRAM+E+VPQSVTKVGPAA GG+ G  GGGG RSPKQM P  RRSK DALTKAALGFRVC
Sbjct: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180

Query: 304 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKN 363
           EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YA FQAFDLVYTL KK 
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240

Query: 364 HMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLA 423
           HMIRH+ RYHFDFF+DQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQMA+ASVSMSFLA
Sbjct: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300

Query: 424 FVAFAISSLISGYNLCTRDHA 439
           FVAFA SSLISGYNLCTRD A
Sbjct: 301 FVAFAFSSLISGYNLCTRDPA 321

BLAST of CmaCh10G001270 vs. ExPASy TrEMBL
Match: A0A0A0LNX3 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.3e-140
Identity = 277/333 (83.18%), Postives = 288/333 (86.49%), Query Frame = 0

Query: 124 MIEAKL---KSND-SSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPY 183
           MIE KL   +SND SSSM+KSSSRNS+ SVHS MESPHSPLRFHSPLR D GD  ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 184 ASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMF 243
           ASPDTSPEK  DNSKAIV VDVVDKSTQFSPLPSPYA+SRK  ENV+F GDRSTSSK+MF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAASRKPLENVNFVGDRSTSSKIMF 120

Query: 244 NRAMKEDVPQSVTKVGP-------------AAGGIGERGGGGERSP-KQMPPMSRRSKDD 303
           NRAMKE+VPQSVTKVGP               GG G  GGGG RSP KQM PMSRRSKDD
Sbjct: 121 NRAMKEEVPQSVTKVGPVGVGGEENGGGGGGGGGAGAGGGGGGRSPVKQMAPMSRRSKDD 180

Query: 304 ALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQ 363
           AL KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYA FQ
Sbjct: 181 ALVKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQ 240

Query: 364 AFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQ 423
           AFDLV+TL KKNHMIRH FR +FDFFMDQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQ
Sbjct: 241 AFDLVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQ 300

Query: 424 MASASVSMSFLAFVAFAISSLISGYNLCTRDHA 439
           +ASASVSMSFLAFVAFA+SSLISGYNLCTRD A
Sbjct: 301 LASASVSMSFLAFVAFAVSSLISGYNLCTRDPA 333

BLAST of CmaCh10G001270 vs. ExPASy TrEMBL
Match: A0A5D3BHR8 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001760 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.7e-140
Identity = 279/330 (84.55%), Postives = 287/330 (86.97%), Query Frame = 0

Query: 124 MIEAKL---KSND-SSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPY 183
           MIE KL   +SND SSSM+KSSSRNS+ SVHS MESPHSPLRFHSPLR D GD  ETPPY
Sbjct: 1   MIETKLRAQRSNDSSSSMKKSSSRNSDSSVHSHMESPHSPLRFHSPLRSDAGDPMETPPY 60

Query: 184 ASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMF 243
           ASPDTSPEK  DNSKAIV VDVVDKSTQFSPLPSPYA SRK  ENV+F GDRSTSSKVMF
Sbjct: 61  ASPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRKPLENVNFVGDRSTSSKVMF 120

Query: 244 NRAMKEDVPQSVTKVGPAA----------GGIGERGGGGERSP-KQMPPMSRRSKDDALT 303
           NRAMKE+VPQSVTKVGPA           GG G  GGGG RSP KQM PMSRRSKDDAL 
Sbjct: 121 NRAMKEEVPQSVTKVGPAGVGGVENGGGDGGGGGGGGGGGRSPVKQMAPMSRRSKDDALM 180

Query: 304 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFD 363
           KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYA FQAFD
Sbjct: 181 KAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFD 240

Query: 364 LVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMAS 423
           LV+TL KKNHMIRH FR +FDFFMDQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQ+AS
Sbjct: 241 LVFTLIKKNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLAS 300

Query: 424 ASVSMSFLAFVAFAISSLISGYNLCTRDHA 439
           ASVSMSFLAFVAFA SSLISGYNLCTRD A
Sbjct: 301 ASVSMSFLAFVAFAFSSLISGYNLCTRDPA 330

BLAST of CmaCh10G001270 vs. NCBI nr
Match: XP_022988377.1 (CASP-like protein 4A2 [Cucurbita maxima])

HSP 1 Score: 740.3 bits (1910), Expect = 9.3e-210
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 57  MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK 116
           MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK
Sbjct: 1   MMPRGFQITNANLETSPPKVLCFLYLFFPDFPNSLFLFILFYLFLFVLCELIFPANLSIK 60

Query: 117 SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET 176
           SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET
Sbjct: 61  SFEVGRKMIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPET 120

Query: 177 PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK 236
           PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK
Sbjct: 121 PPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSK 180

Query: 237 VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV 296
           VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV
Sbjct: 181 VMFNRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRV 240

Query: 297 CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK 356
           CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK
Sbjct: 241 CEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKK 300

Query: 357 NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL 416
           NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL
Sbjct: 301 NHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFL 360

Query: 417 AFVAFAISSLISGYNLCTRDHA 439
           AFVAFAISSLISGYNLCTRDHA
Sbjct: 361 AFVAFAISSLISGYNLCTRDHA 382

BLAST of CmaCh10G001270 vs. NCBI nr
Match: XP_022921997.1 (CASP-like protein 4A2 [Cucurbita moschata] >KAG6589449.1 CASP-like protein 4A2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023131.1 CASP-like protein 4A2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595.9 bits (1535), Expect = 2.8e-166
Identity = 308/315 (97.78%), Postives = 309/315 (98.10%), Query Frame = 0

Query: 124 MIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPD 183
           MIEAKLKS DSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLR DVGDLPETPPYASPD
Sbjct: 1   MIEAKLKSIDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRSDVGDLPETPPYASPD 60

Query: 184 TSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM 243
           TSPEKQ DNSKAIV VDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM
Sbjct: 61  TSPEKQPDNSKAIVLVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM 120

Query: 244 KEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 303
           KEDVPQSVTKVGPAAGG+ ERGGGG RSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL
Sbjct: 121 KEDVPQSVTKVGPAAGGVEERGGGGGRSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 180

Query: 304 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH 363
           ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH
Sbjct: 181 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH 240

Query: 364 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 423
           FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI
Sbjct: 241 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 300

Query: 424 SSLISGYNLCTRDHA 439
           SSLISGYNLCTRDHA
Sbjct: 301 SSLISGYNLCTRDHA 315

BLAST of CmaCh10G001270 vs. NCBI nr
Match: XP_023515843.1 (CASP-like protein 4A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.6 bits (1529), Expect = 1.4e-165
Identity = 306/315 (97.14%), Postives = 308/315 (97.78%), Query Frame = 0

Query: 124 MIEAKLKSNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPD 183
           MIEAKLKS DSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLR DVGDLPETPPYASPD
Sbjct: 1   MIEAKLKSIDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRSDVGDLPETPPYASPD 60

Query: 184 TSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFNRAM 243
           TSPEKQ DNSKAIV VDVVDKSTQFSPLPSPYASSRKTPENVSFPGD+STSSKVMFNRAM
Sbjct: 61  TSPEKQPDNSKAIVVVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDKSTSSKVMFNRAM 120

Query: 244 KEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 303
           KEDVPQSVTKVGPAAGG+ ERGGGG RSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL
Sbjct: 121 KEDVPQSVTKVGPAAGGVEERGGGGGRSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCL 180

Query: 304 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHH 363
           ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNII FVYAVFQAFDLVYTLFKKNHMIRHH
Sbjct: 181 ISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIGFVYAVFQAFDLVYTLFKKNHMIRHH 240

Query: 364 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 423
           FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI
Sbjct: 241 FRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAI 300

Query: 424 SSLISGYNLCTRDHA 439
           SSLISGYNLCTRDHA
Sbjct: 301 SSLISGYNLCTRDHA 315

BLAST of CmaCh10G001270 vs. NCBI nr
Match: XP_038878399.1 (CASP-like protein 4A2 [Benincasa hispida])

HSP 1 Score: 526.6 bits (1355), Expect = 2.1e-145
Identity = 283/323 (87.62%), Postives = 289/323 (89.47%), Query Frame = 0

Query: 124 MIEAKLK---SNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYA 183
           MIE KLK   SNDSSSM+KSSSRNSE SVHS MESPHSPLRFHSPLR D GD PETPPYA
Sbjct: 1   MIETKLKAQRSNDSSSMKKSSSRNSESSVHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 184 SPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDRSTSSKVMFN 243
           SPDTSPEK  DNSKAIV VDVVDKSTQFSPLPSPYA SR  PENV+F GDRSTSSKVMFN
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYAGSRMPPENVNFTGDRSTSSKVMFN 120

Query: 244 RAMKEDVPQSVTKVGPAAGGIGER----GGGGERSP-KQMPPMSRRSKDDALTKAALGFR 303
           RAMKE+VPQSVTKVGPA GG  E     GGGG RSP KQM PMSRRSKDDALTKAALGFR
Sbjct: 121 RAMKEEVPQSVTKVGPAGGGGEENGGGGGGGGGRSPVKQMAPMSRRSKDDALTKAALGFR 180

Query: 304 VCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFK 363
           VCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYA FQAFDLV+TL K
Sbjct: 181 VCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAAFQAFDLVFTLIK 240

Query: 364 KNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSF 423
           KNHMIRH FR +FDFFMDQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQ+ASASVSMSF
Sbjct: 241 KNHMIRHQFRCYFDFFMDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQLASASVSMSF 300

Query: 424 LAFVAFAISSLISGYNLCTRDHA 439
           LAFVAFA SSLISGYNLCTRD A
Sbjct: 301 LAFVAFAFSSLISGYNLCTRDPA 323

BLAST of CmaCh10G001270 vs. NCBI nr
Match: XP_022135555.1 (CASP-like protein 4A2 [Momordica charantia])

HSP 1 Score: 521.2 bits (1341), Expect = 8.9e-144
Identity = 278/321 (86.60%), Postives = 288/321 (89.72%), Query Frame = 0

Query: 124 MIEAKLK---SNDSSSMRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYA 183
           MIE KLK   SNDSSSMRKSSSRNSE S+HS MESPHSPLRFHSPLR D GD PETPPYA
Sbjct: 1   MIETKLKTQRSNDSSSMRKSSSRNSESSLHSHMESPHSPLRFHSPLRSDAGDPPETPPYA 60

Query: 184 SPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTP-ENVSFPGDRSTSSKVMF 243
           SPDTSPEK  DNSKAIV VDVVDKSTQFSPLPSPYA SRK P EN +FP DRSTSSKVMF
Sbjct: 61  SPDTSPEKLPDNSKAIVVVDVVDKSTQFSPLPSPYADSRKPPAENANFPADRSTSSKVMF 120

Query: 244 NRAMKEDVPQSVTKVGPAA-GGI-GERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVC 303
           NRAM+E+VPQSVTKVGPAA GG+ G  GGGG RSPKQM P  RRSK DALTKAALGFRVC
Sbjct: 121 NRAMREEVPQSVTKVGPAATGGVEGGGGGGGGRSPKQMAPAPRRSKADALTKAALGFRVC 180

Query: 304 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKN 363
           EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVN+IAF+YA FQAFDLVYTL KK 
Sbjct: 181 EVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNVIAFIYAAFQAFDLVYTLIKKK 240

Query: 364 HMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLA 423
           HMIRH+ RYHFDFF+DQ LAYLLISSSSSAATRVDDWQSNWGKDEFTQMA+ASVSMSFLA
Sbjct: 241 HMIRHYLRYHFDFFVDQVLAYLLISSSSSAATRVDDWQSNWGKDEFTQMATASVSMSFLA 300

Query: 424 FVAFAISSLISGYNLCTRDHA 439
           FVAFA SSLISGYNLCTRD A
Sbjct: 301 FVAFAFSSLISGYNLCTRDPA 321

BLAST of CmaCh10G001270 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 259.2 bits (661), Expect = 5.9e-69
Identity = 164/310 (52.90%), Postives = 207/310 (66.77%), Query Frame = 0

Query: 137 MRKSSSRNSELSVHSQMESPHSPLRFHSPLRFDVGDLPETPPYASPDTSPEKQTDNSKAI 196
           M++++S NSE    S  ESPHSPLRFHSPL  D GDLPE+  Y SP+ SP K  +N K+I
Sbjct: 3   MKRTASSNSE--AQSYNESPHSPLRFHSPLS-DAGDLPES-RYVSPEGSPFK-IENPKSI 62

Query: 197 VAVDVVDKSTQFSPLPSPY------------ASSRKTPENVSFPGDRSTSSKVMFNRAMK 256
           VA    +K TQFSPLPSP               + + P N S   D+S SS V+ N  ++
Sbjct: 63  VA---GNKLTQFSPLPSPIPPPPPQFPPPRRQRNARVPMNSS--SDKSPSSMVVHNSWVR 122

Query: 257 EDVPQSVTKVGPAAGGIGERGGGGERSPKQMPPMSRRSKDDALTKAALGFRVCEVAVCLI 316
           ED  Q+ T+   A          GE S +    ++R   DD ++  ALGFR+ EV +C+I
Sbjct: 123 EDGGQTTTRKAGAP-------INGEESTR--TTVNRARGDDLVSLTALGFRITEVILCVI 182

Query: 317 SFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLFKKNHMIRHHF 376
           SFS+MA+DKTQGWSGDS+DRYKEYRYCL VN+IAFVY+ F+A D    + K+++MI   F
Sbjct: 183 SFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVIAFVYSAFEACDAACYIAKESYMINCGF 242

Query: 377 RYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMASASVSMSFLAFVAFAIS 435
              F F MDQ LAYLL+S+SS AATRVDDW SNWGKDEFTQMA+AS+++SFLAF AFA+S
Sbjct: 243 HDLFVFSMDQLLAYLLMSASSCAATRVDDWVSNWGKDEFTQMATASIAVSFLAFGAFAVS 293

BLAST of CmaCh10G001270 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 228.4 bits (581), Expect = 1.1e-59
Identity = 137/274 (50.00%), Postives = 179/274 (65.33%), Query Frame = 0

Query: 175 ETPPYASPDT-SPEKQ---TDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGD 234
           +T P  SP + S EK    +D S AIVA D  + +T FS  PSP  S   + E+      
Sbjct: 6   KTMPSMSPSSVSTEKSPPPSDTSMAIVAFD--NSTTHFSSSPSPPHSLDHSSESEKEDAK 65

Query: 235 RSTSSKVMFNRAMKEDVP----------QSVTKVGPA--AGGIGERGGGGERSPKQMPPM 294
               S+   N    E+ P          +SV +V P   +  +G     G+RS      +
Sbjct: 66  SKPESRRNKNPGKVEETPSPIVVVHNHNRSVKEVVPTRKSARVGSGRSSGQRSGAVSAIL 125

Query: 295 SRRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIA 354
            R  +++ +  +ALGFR+ EV + LISFS+MA+DKT+GWSGDSFDRYKEYR+CL+VN++A
Sbjct: 126 RRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSVNVVA 185

Query: 355 FVYAVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNW 414
           FVY+ FQA DL Y L K+ H+I HH R  F+F +DQ LAYLL+S+S++A TRVDDW SNW
Sbjct: 186 FVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDWVSNW 245

Query: 415 GKDEFTQMASASVSMSFLAFVAFAISSLISGYNL 433
           GKDEFT+MASAS++MSFLAF+AFA SSLISGYNL
Sbjct: 246 GKDEFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of CmaCh10G001270 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 159.1 bits (401), Expect = 8.3e-39
Identity = 106/272 (38.97%), Postives = 146/272 (53.68%), Query Frame = 0

Query: 172 DLPETPPYASPDTSPEKQTDNSKAIVAVDVVDKSTQFSPLPSPYASSRKTPENVSFPGDR 231
           +L +T  +       +++ D S  I   ++  +S+  S   +   S   +P   S P   
Sbjct: 3   ELEKTQKFQKKKKQQQEKQDQSSPI-NFEMSSRSSLHSLPQTTIESPPDSPTLSSIPDSH 62

Query: 232 STSSKVMF--NRAMKEDVPQSVTKVGPAAGGIGERGGGGERSPKQMPP-------MSRRS 291
            +S   +       K + P  VT         GE       S +Q+ P         R S
Sbjct: 63  GSSPHTIIPTPSVAKTETPFRVTN--------GEEEKKVSESRRQLRPSFSSSSSTPRES 122

Query: 292 K-DDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAFVY 351
           K    + KA LGFRV     CL+SFSVM SD+ +GW+ DSF  YKE+R+CL  N+I FVY
Sbjct: 123 KWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVY 182

Query: 352 AVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKD 411
           + F   DLVY L       RH+ R+  +F +DQ LAYLL S+S+SA+ RVDDWQSNWG D
Sbjct: 183 SGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGAD 242

Query: 412 EFTQMASASVSMSFLAFVAFAISSLISGYNLC 434
           +F  +A ASV++S+++FVAFA  SL SGY LC
Sbjct: 243 KFPDLARASVALSYVSFVAFAFCSLASGYALC 265

BLAST of CmaCh10G001270 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 80.5 bits (197), Expect = 3.7e-15
Identity = 58/156 (37.18%), Postives = 91/156 (58.33%), Query Frame = 0

Query: 280 RRSKDDALTKAALGFRVCEVAVCLISFSVMASDKTQGWSGDSFDRYKEYRYCLTVNIIAF 339
           R  ++D + KA+   R   +   LI+F +M S+K  G+ G +F+ Y+EYRY L ++II+ 
Sbjct: 37  RWKREDLIKKASPITRGICLLFSLIAFLIMVSNK-HGY-GRNFNDYEEYRYVLAISIIST 96

Query: 340 VYAVFQAFDLVYTLFKKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWG 399
           +Y  +Q F      F K  +         DF  DQ +AYLLIS++SSA    + ++   G
Sbjct: 97  LYTAWQTF----AHFSKREIFDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFRE--G 156

Query: 400 KDE-FTQMASASVSMSFLAFVAFAISSLISGYNLCT 435
           +D  FT  A++++SM+  AF+A A+S+L SGY L T
Sbjct: 157 QDNIFTDSAASAISMAIFAFIALALSALFSGYKLST 184

BLAST of CmaCh10G001270 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 68.2 bits (165), Expect = 1.9e-11
Identity = 48/144 (33.33%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 296 VCEVAVCLISFSVMASDKTQ-GWSGDSFDRYKEYRYCLTVNIIAFVYAVFQAFDLVYTLF 355
           +C V+   ++ +V    K     +  SF  Y E  YC  V +I FVY   Q F  V  + 
Sbjct: 66  LCFVSALSLAVNVQRPSKRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDIT 125

Query: 356 KKNHMIRHHFRYHFDFFMDQALAYLLISSSSSAATRVDDWQSNWGKDEFTQMAS---ASV 415
            +  +I      +  F  DQ + YLL+SSSS A      W  +  +D    + +    SV
Sbjct: 126 HRGVLISEPLSDYISFIFDQVICYLLVSSSSVAIA----WIQHINEDAIKTLRNNSIVSV 185

Query: 416 SMSFLAFVAFAISSLISGYNLCTR 436
           SMSF AF+   +S L+SGY LC R
Sbjct: 186 SMSFSAFLVLTLSGLLSGYKLCKR 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q501G68.2e-6852.90CASP-like protein 4A2 OS=Arabidopsis thaliana OX=3702 GN=At5g62820 PE=2 SV=1[more]
D7MMW41.4e-6752.58CASP-like protein 4A2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q84WP51.6e-5850.00CASP-like protein 4A3 OS=Arabidopsis thaliana OX=3702 GN=At2g36330 PE=2 SV=1[more]
D7LIR21.7e-5747.31CASP-like protein 4A3 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q9FNE81.2e-3738.97CASP-like protein 4A1 OS=Arabidopsis thaliana OX=3702 GN=At5g40300 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JCW14.5e-210100.00CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111485636 PE=3 SV=1[more]
A0A6J1E1W91.4e-16697.78CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111430082 PE=3 SV=1[more]
A0A6J1C1D14.3e-14486.60CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111007480 PE=3 SV=1[more]
A0A0A0LNX31.3e-14083.18CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G004970 PE=3 SV=1[more]
A0A5D3BHR81.7e-14084.55CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00... [more]
Match NameE-valueIdentityDescription
XP_022988377.19.3e-210100.00CASP-like protein 4A2 [Cucurbita maxima][more]
XP_022921997.12.8e-16697.78CASP-like protein 4A2 [Cucurbita moschata] >KAG6589449.1 CASP-like protein 4A2, ... [more]
XP_023515843.11.4e-16597.14CASP-like protein 4A2 [Cucurbita pepo subsp. pepo][more]
XP_038878399.12.1e-14587.62CASP-like protein 4A2 [Benincasa hispida][more]
XP_022135555.18.9e-14486.60CASP-like protein 4A2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G62820.15.9e-6952.90Uncharacterised protein family (UPF0497) [more]
AT2G36330.11.1e-5950.00Uncharacterised protein family (UPF0497) [more]
AT5G40300.18.3e-3938.97Uncharacterised protein family (UPF0497) [more]
AT2G38480.13.7e-1537.18Uncharacterised protein family (UPF0497) [more]
AT4G11655.11.9e-1133.33Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 285..420
e-value: 7.5E-32
score: 110.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 208..234
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..158
NoneNo IPR availablePANTHERPTHR11615:SF251CASP-LIKE PROTEINcoord: 193..436
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 193..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G001270.1CmaCh10G001270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding