CmaCh08G006770 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G006770
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRHOMBOID-like protein
LocationCma_Chr08: 4164645 .. 4167124 (-)
RNA-Seq ExpressionCmaCh08G006770
SyntenyCmaCh08G006770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTCGTCACCGACGCTATTGTGCACTCTTCCCCTCCGCCGCGCAATGGGGAAGAGGTCGCCGCCGGGCGACATCGAGTCCATGTCCTATGATGTCCGAAAGCCCCTCTCAATGCCGCCACCGCCAAGCTATTACCCTCCGCCTCCCAAGCCCTGGTTCCCATGGCTTGTCCCCCTCACTTTCCTGGCCAATATTGCCATGTTTGTTTTCACATTATATGAAAATAATTGCCCCGCGGCCATTGGCGGCAACCGCTGCCTCTTTTACCCTGAGCTCGGCCGGTTCTCCTTCCAGCCATTTCAGGAGAACCCCCTCCTCGGCCCTTCCATCAGCACGTAAGAATCTATTGATTCGGGTGTTCGTCTAATCGAAAAATTAGCATATGGGTTTGGTAAATATGGATGAAATTGGACGACCCATCAAGAGAACATGATTTTTGGGGGGAAATTCTGGTTTTAGCAGAAGGGGAAGAACAGAGGAACAGAAGAACAGGGGAGTTTTGTTTGATTTTCTTTTGTTCTTTGTATTTTGCAGTTTGAAACGATATGGGGCATTGGATAAAGAAGCAGTAGTGGAACATGGCGAAGGGTGGCGCTTGGTTTCGTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGGCTCGAACAAGAATTTGGATTTTGTAAGCACTTTCAACACTGTAAACTGCTTTGTTTTTTTTTTTTATGTGTTGATTCATAGTTTCACGTTGCACACTCTACAAACATATGTAGTTCAAACACTAAGAACTAAGAACAAGAGGGAGGGAGAGATTTTGCCTGTGTTTTTAGCAAGAGTTAAGTTACTTTGATTGTTTATTACAAGAATTTTGAAAAGTGATTTTATATTAAGTTTGCCCAAGTCAGATATGGTTCGTTTTGGCTCGTTACGTATCACCGTTAGCATCACAGTTTTAAAACATTTTTGTTAGAGAGAGGTTTTCACACCCTTATAAAGAATGTGGAGACCTCTCCAATTGATAATCCAGGGGTGGATTGTAAAATCTCACATCGGTTGGAGAGGGGACTAAGCATTTCTTACAAGGGTGTGGAAACCTCTCCCTAACAAACACATTTTAAAACTGTGAAGCTGACGGCGATATATAACGAACCGAAATGGACAATATATACTAGCAGTAGACTTGGGACATTACAAATGATATTAGAGTCAAACACCAGGCGGTGTGCCAGTGAGGACGTTGGGCCCCCAAAGGGGGTGGTTTGTGAGATCAAGTGTGGAAACTTTTCTCTAGTAAACATGTTCTAAAACCGTGAGGTTGGCGGCAATATATAATGGGCTAAAGCGGATAATATCTATTAGCAGTGAGTTTGGAGTGTTACAATCAAATATTTGTTAAGTCGAGAGTAAATATACATAAACTCATCGACATTAAATTTCTTTTTTTGTATAAAAATAATACATACGTTTTTCTTAAAAACTAATGTGTTGTCAGTTATGGTGGTGTACGTGTCATATTCATGTCTCGTATCGATATCCGTGCTTGTTAGAAAATAATGTTTGGTTCTTGTTTTTGGTTTTGGCAGTGAAAATAGGATGTTTGTATGTGCTTTCTGGATTTGGTGGGAGCTTACTTTCATCTCTGAGTATCAATCCATCTGAAAATCCAATCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGCGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATTCAAATAAGGTAACAAAAAAGAAACAAATAATCCTTTTTCCATTCCATCTTTCCATACTATACATTGGGACCTTATGGCTTGTTTGTCATTCATACTCAGTGTGCAGCCTTCATATCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTCATACCTCACATCGACAATTCAGCACACATTGGAGGCTTCGTTTCGGGCTTTCTCCTCGGCTTCATCCTCCTTATCCAACCGCAGTTCGGATACGTTAACCATAAGTACATCCCTGCAGGGGCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATCGTAGCTCTAATCCTCTTGATTTTTGGGTAAGCAAAACCGAAGCGACCGATATACTTTCCGTCTAGCTCGAGTTTTATCTTGTAACTTTTTTTGCCATTTTTGTAGATATGTATCTGGTTTAACAAGGCTTTACAAAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGAAGTGGCTGTTCGTGGGAAGCAAAAGTACTTTGATTTGGTGATCGTTTGGATGAGGAAAGCTTATATGATTTTCCTTTGAAAGTGTAATTTAAACGATAAAATCATAATGTGAAGTTTAACTTGTATGTAGAAAATACCCACTTCTCCTTTTGTTTTATAGATCTCTGTTCAGCAATAAAAGCGTTACAAGGCAAAACAAACAATTACAAAG

mRNA sequence

CCGTCGTCACCGACGCTATTGTGCACTCTTCCCCTCCGCCGCGCAATGGGGAAGAGGTCGCCGCCGGGCGACATCGAGTCCATGTCCTATGATGTCCGAAAGCCCCTCTCAATGCCGCCACCGCCAAGCTATTACCCTCCGCCTCCCAAGCCCTGGTTCCCATGGCTTGTCCCCCTCACTTTCCTGGCCAATATTGCCATGTTTGTTTTCACATTATATGAAAATAATTGCCCCGCGGCCATTGGCGGCAACCGCTGCCTCTTTTACCCTGAGCTCGGCCGGTTCTCCTTCCAGCCATTTCAGGAGAACCCCCTCCTCGGCCCTTCCATCAGCACTTTGAAACGATATGGGGCATTGGATAAAGAAGCAGTAGTGGAACATGGCGAAGGGTGGCGCTTGGTTTCGTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGGCTCGAACAAGAATTTGGATTTTTGAAAATAGGATGTTTGTATGTGCTTTCTGGATTTGGTGGGAGCTTACTTTCATCTCTGAGTATCAATCCATCTGAAAATCCAATCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGCGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATTCAAATAAGTGTGCAGCCTTCATATCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTCATACCTCACATCGACAATTCAGCACACATTGGAGGCTTCGTTTCGGGCTTTCTCCTCGGCTTCATCCTCCTTATCCAACCGCAGTTCGGATACGTTAACCATAAGTACATCCCTGCAGGGGCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATCGTAGCTCTAATCCTCTTGATTTTTGGATATGTATCTGGTTTAACAAGGCTTTACAAAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGAAGTGGCTGTTCGTGGGAAGCAAAAGTACTTTGATTTGGTGATCGTTTGGATGAGGAAAGCTTATATGATTTTCCTTTGAAAGTGTAATTTAAACGATAAAATCATAATGTGAAGTTTAACTTGTATGTAGAAAATACCCACTTCTCCTTTTGTTTTATAGATCTCTGTTCAGCAATAAAAGCGTTACAAGGCAAAACAAACAATTACAAAG

Coding sequence (CDS)

ATGGGGAAGAGGTCGCCGCCGGGCGACATCGAGTCCATGTCCTATGATGTCCGAAAGCCCCTCTCAATGCCGCCACCGCCAAGCTATTACCCTCCGCCTCCCAAGCCCTGGTTCCCATGGCTTGTCCCCCTCACTTTCCTGGCCAATATTGCCATGTTTGTTTTCACATTATATGAAAATAATTGCCCCGCGGCCATTGGCGGCAACCGCTGCCTCTTTTACCCTGAGCTCGGCCGGTTCTCCTTCCAGCCATTTCAGGAGAACCCCCTCCTCGGCCCTTCCATCAGCACTTTGAAACGATATGGGGCATTGGATAAAGAAGCAGTAGTGGAACATGGCGAAGGGTGGCGCTTGGTTTCGTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGGCTCGAACAAGAATTTGGATTTTTGAAAATAGGATGTTTGTATGTGCTTTCTGGATTTGGTGGGAGCTTACTTTCATCTCTGAGTATCAATCCATCTGAAAATCCAATCATATCAGTTGGTGCTTCTGGTGCACTTTTTGGGCTTTTGGGCGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATTCAAATAAGTGTGCAGCCTTCATATCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTCATACCTCACATCGACAATTCAGCACACATTGGAGGCTTCGTTTCGGGCTTTCTCCTCGGCTTCATCCTCCTTATCCAACCGCAGTTCGGATACGTTAACCATAAGTACATCCCTGCAGGGGCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATCGTAGCTCTAATCCTCTTGATTTTTGGATATGTATCTGGTTTAACAAGGCTTTACAAAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGA

Protein sequence

MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSGLTRLYKTGPQVLESNTF
Homology
BLAST of CmaCh08G006770 vs. ExPASy Swiss-Prot
Match: Q9SSR0 (RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-96
Identity = 182/310 (58.71%), Postives = 230/310 (74.19%), Query Frame = 0

Query: 1   MGKRSP-PGDIESMSYDVRKPLSMPPP--PSYYPPPPKPWFPWLVPLTFLANIAMFVFTL 60
           MGKR P P DIE+           PPP  P + PP P PW  WLVPL   AN   F  T+
Sbjct: 1   MGKRPPIPPDIEN---------GPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTM 60

Query: 61  YENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWR 120
           Y N+CPA    + CL +  LGR SFQP +EN LLGPSI TL++ GAL++  +VE GE WR
Sbjct: 61  YVNDCPAR--SDECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGERWR 120

Query: 121 LVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSE 180
           L+SC+WLH G +HL+ANM+SL+ IG+RLEQEFGF++IG LYV+SG GGSL+S L+ +  E
Sbjct: 121 LISCIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDSQGE 180

Query: 181 NPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSA 240
              +SVGASGALFGLLGAMLSELITNWTIY NKC A ++LILII LNL+VGF+P +DNSA
Sbjct: 181 R--VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSA 240

Query: 241 HIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVK-RKSKHKCYQYFLLIVALILLIFG 300
           H GGF++GF LGF+LL++PQ+GYVN KYIP G D+K +KSKHKCYQ+     +L +L+ G
Sbjct: 241 HFGGFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAG 296

Query: 301 YVSGLTRLYK 307
           +++G T+L +
Sbjct: 301 FIAGYTKLLR 296

BLAST of CmaCh08G006770 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.6e-89
Identity = 173/275 (62.91%), Postives = 206/275 (74.91%), Query Frame = 0

Query: 33  PPKPWFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLG 92
           P K WFPWLVP   +ANIA+F  +++ NNCP         F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 93  PSISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCA 212
           +IG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK A
Sbjct: 170 RIGLLYMISGFGGSLLSSLFNRAG----ISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 AFISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAG-AD 272
           A ++LI IIA+NLAVG +PH+DN AH+GGF SGFLLGF+ LI+PQ+GY N +  P G A 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
              KSKHK YQY L I +L+LLI GY +GL  L +
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLR 317

BLAST of CmaCh08G006770 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.6e-83
Identity = 156/273 (57.14%), Postives = 206/273 (75.46%), Query Frame = 0

Query: 37  WFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFY---PELGRFSFQPFQENPLLGP 96
           W  WLVP+  +AN+A+FV  ++ NNCP     +R   +     LGR SF+P + NPL GP
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGP 112

Query: 97  SISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLK 156
           S  TL++ GAL+   VVE  EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF++
Sbjct: 113 SSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVR 172

Query: 157 IGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAA 216
           IG +Y+LSG GGS+LSSL I  S    ISVGASGALFGLLG+MLSEL TNWTIYSNK AA
Sbjct: 173 IGVIYLLSGIGGSVLSSLFIRNS----ISVGASGALFGLLGSMLSELFTNWTIYSNKIAA 232

Query: 217 FISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVK 276
            ++L+ +I +NLA+G +PH+DN AH+GGFV+GFLLGFILL +PQF ++  +++P G  + 
Sbjct: 233 LLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL- 292

Query: 277 RKSKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
            + K+K YQY L +++L+LLI G+V  L  L++
Sbjct: 293 -RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

BLAST of CmaCh08G006770 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.5e-82
Identity = 152/271 (56.09%), Postives = 203/271 (74.91%), Query Frame = 0

Query: 37  WFPWLVPLTFLANIAMFVFTLYENNCPAAI-GGNRCLFYPELGRFSFQPFQENPLLGPSI 96
           W  WL+P   +AN+A+F+  ++ N+CP  I G N+      LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  STLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIG 156
           STL++ GAL+   VV   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+++G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFI 216
            +Y++SG GGS+LSSL +  S    ISVGASGALFGLLGAMLSEL+TNWTIY+NK AA I
Sbjct: 151 LIYLISGLGGSILSSLFLQES----ISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRK 276
           +L+ IIA+NLA+G +P +DN AHIGGF++GF LGF+LL++PQ+G+   +   +    + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASRTNTS----RTK 270

Query: 277 SKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
            K+  YQY L +V+++LL+ G    L  L+K
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

BLAST of CmaCh08G006770 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.0e-80
Identity = 148/271 (54.61%), Postives = 198/271 (73.06%), Query Frame = 0

Query: 35  KPWFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPS 94
           + WFPWL+P   +AN+A+FV T+Y NNCP   G     F   LGRFSFQ  +ENPLLGPS
Sbjct: 62  RSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGD---CFADFLGRFSFQNTRENPLLGPS 121

Query: 95  ISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKI 154
             TL+  G LD + VV+  EGWRL+SC WLH GV+HLL NML+LLFIGIR+E+EFGF++I
Sbjct: 122 SLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRI 181

Query: 155 GCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAF 214
           G LY++SGFGGS+LS+L +  +    ISVGASGA+FGLLG MLSE+  NWTIYSNK    
Sbjct: 182 GLLYLISGFGGSILSALFLRSN----ISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTI 241

Query: 215 ISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKR 274
           ++L+LI+A+NL +G +P +DN AHIGGF +GFLLGF+LLI+P +G++N +  P      +
Sbjct: 242 VTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGPGA----K 301

Query: 275 KSKHKCYQYFLLIVALILLIFGYVSGLTRLY 306
             + K YQ  L  ++L++L+ G++ GL  L+
Sbjct: 302 PHRFKIYQGILWTISLLILVAGFIVGLISLF 321

BLAST of CmaCh08G006770 vs. ExPASy TrEMBL
Match: A0A6J1KMF5 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.7e-180
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LTRLYKTGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of CmaCh08G006770 vs. ExPASy TrEMBL
Match: A0A6J1HKQ1 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 4.3e-178
Identity = 313/317 (98.74%), Postives = 314/317 (99.05%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKRSPPGDIESMSY+VRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LTRLYKTGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of CmaCh08G006770 vs. ExPASy TrEMBL
Match: A0A5A7UZ09 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002320 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.5e-157
Identity = 271/319 (84.95%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLS-MPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYE 60
           MGKR P  D+ESMSY++ KP++ MPPPPSYYPPPPKPWFPWLVPL FL N+ MF+FTLY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLV 120
           NNCPA  GGNRCL YPELGRFSFQPF ENPLLGP+IS L+RYGALDKEAVVE GEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFLKIGCLYVLSGFGGSLLSS+S+NP+ENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 IISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHI 240
            ISVGASGALFGLLGAMLSELITNWTIY+NKCAA +SLILIIALNLAVGF+PH+DNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVS 300
           GGF+SGFLLGFILLI+PQFGYVNHKYIP G+DVKRKSKHKCYQYFLLIVAL+LL+FGY+S
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFGYIS 300

Query: 301 GLTRLYKTGP-QVLESNTF 318
           GLT+LY+TGP QV +SN F
Sbjct: 301 GLTKLYRTGPIQVSKSNRF 319

BLAST of CmaCh08G006770 vs. ExPASy TrEMBL
Match: A0A6J1JR47 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488148 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.0e-156
Identity = 273/317 (86.12%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGK+ P  DIESMSY VRKPLSMPPPPSYYPPPPKPWFP+LVPL F+A+IA+FVFTLYEN
Sbjct: 1   MGKKLPRDDIESMSYGVRKPLSMPPPPSYYPPPPKPWFPFLVPLIFVADIALFVFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  GGNRCLFYPEL R+SFQPF ENPLLGPSI TLKRYGALD+EAVVE GEGWRL++
Sbjct: 61  NCPATTGGNRCLFYPELDRYSFQPFSENPLLGPSIMTLKRYGALDREAVVERGEGWRLLT 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANM+SLLFIG+RLEQEFG+L+IGCLYVLSGFGGSLLS LSINP+E   
Sbjct: 121 CMWLHAGVIHLLANMISLLFIGVRLEQEFGYLRIGCLYVLSGFGGSLLSCLSINPAET-T 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGA+LSELITNWTIYSNKCAA +SLILIIALNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGALLSELITNWTIYSNKCAAMMSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVN KYIPAG DVKRKSKH+CYQYFLL+VALILLIFGY SG
Sbjct: 241 GFLSGFLLGFILLIRPQFGYVNPKYIPAGCDVKRKSKHQCYQYFLLLVALILLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           L+RLYKTGPQ+LE+N F
Sbjct: 301 LSRLYKTGPQILEANIF 316

BLAST of CmaCh08G006770 vs. ExPASy TrEMBL
Match: A0A6J1GPL6 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111456374 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.8e-155
Identity = 273/317 (86.12%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGK+ P  DIESMSY VRKPLSMPPPPSYYPPPPKPWFP+LVPL F+A+IA+FVFTLYEN
Sbjct: 1   MGKKLPRDDIESMSYGVRKPLSMPPPPSYYPPPPKPWFPFLVPLIFVADIALFVFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  GGNRCLFYPEL R+SFQPF ENPLLGPSI TLKRYGALD+EAVVE GEGWRL++
Sbjct: 61  NCPATTGGNRCLFYPELDRYSFQPFSENPLLGPSIMTLKRYGALDREAVVERGEGWRLLT 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANM+SLLFIG+RLEQEFG+L+IGCLYVLSGFGGSLLS LSINP+E   
Sbjct: 121 CMWLHAGVIHLLANMISLLFIGVRLEQEFGYLRIGCLYVLSGFGGSLLSCLSINPAET-T 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGA+LSELITNWTIYSNKCAA +SLILIIALNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGALLSELITNWTIYSNKCAAMMSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVN KYIPAG DVKRKSKH+CYQYFLLIVALILLIFGY SG
Sbjct: 241 GFLSGFLLGFILLIRPQFGYVNPKYIPAGCDVKRKSKHQCYQYFLLIVALILLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           L+RLYKTGPQ+LE++ F
Sbjct: 301 LSRLYKTGPQILEAHFF 316

BLAST of CmaCh08G006770 vs. NCBI nr
Match: XP_023000373.1 (RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 641.0 bits (1652), Expect = 5.6e-180
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LTRLYKTGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of CmaCh08G006770 vs. NCBI nr
Match: XP_022964405.1 (RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025779.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 633.6 bits (1633), Expect = 8.9e-178
Identity = 313/317 (98.74%), Postives = 314/317 (99.05%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKRSPPGDIESMSY+VRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LTRLYKTGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of CmaCh08G006770 vs. NCBI nr
Match: XP_023514894.1 (RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633.3 bits (1632), Expect = 1.2e-177
Identity = 312/317 (98.42%), Postives = 314/317 (99.05%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKRSPPGDIESMSY+VRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFAFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSI+TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSITTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LTRLYKTGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of CmaCh08G006770 vs. NCBI nr
Match: XP_038899775.1 (RHOMBOID-like protein 5 [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 272/317 (85.80%), Postives = 295/317 (93.06%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKR P   +ESMSYDV KP+SMPPPPSYYPPPPKPWFPWLVPL F+AN+AMF+FTLYEN
Sbjct: 1   MGKRPP--SVESMSYDVHKPMSMPPPPSYYPPPPKPWFPWLVPLIFIANVAMFIFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPS++ L+ YGALDKEAVVE GEGWRLVS
Sbjct: 61  NCPATTGRNHCLLYPELGRFSFQPFQENPLLGPSMNILRIYGALDKEAVVERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           C+WLHAGVIHL+ANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLS+N +E+P 
Sbjct: 121 CIWLHAGVIHLIANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSLNSAESPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NKCAA +SLILII LNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIGLNLAVGFIPHVDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIP G+DVKRKSKHKCYQYFLLI+AL+LLIFGY SG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNHKYIPVGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           LT+LY+TGPQVL SN F
Sbjct: 301 LTKLYRTGPQVLTSNIF 315

BLAST of CmaCh08G006770 vs. NCBI nr
Match: XP_038888787.1 (RHOMBOID-like protein 5 [Benincasa hispida])

HSP 1 Score: 565.5 bits (1456), Expect = 3.0e-157
Identity = 271/317 (85.49%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60
           MGKR P  D+ESMSYDV KP+SMPPPPSYYPPPPKPWFPWLVPL F+AN+AMFVFTLYEN
Sbjct: 1   MGKRLPSVDMESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFIANVAMFVFTLYEN 60

Query: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA    NRCL YPELGRFSFQPFQENPLLGPS++ LKRYGALDK+AVVE GE WRLVS
Sbjct: 61  NCPATTRRNRCLLYPELGRFSFQPFQENPLLGPSMNILKRYGALDKDAVVEQGEAWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180
           C+WLHAGVIHLLANMLSLLFIG+RLEQEFGFLKIGCLY+LSGFGGSLLSSLS+  +E+P 
Sbjct: 121 CIWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYMLSGFGGSLLSSLSLVSAESPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NKCAA ISLILII LNLAVGF+P++DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALISLILIIGLNLAVGFVPNVDNSAHIG 240

Query: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300
           GF+SGFLLGF+LLI+PQFGYVNHKYIP GADVKRKSKHKCYQYFLLI+AL+LL+FGY SG
Sbjct: 241 GFISGFLLGFVLLIRPQFGYVNHKYIPPGADVKRKSKHKCYQYFLLIIALLLLVFGYASG 300

Query: 301 LTRLYKTGPQVLESNTF 318
           L+RLY+TGPQVL SNTF
Sbjct: 301 LSRLYRTGPQVLTSNTF 317

BLAST of CmaCh08G006770 vs. TAIR 10
Match: AT1G52580.1 (RHOMBOID-like protein 5 )

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-97
Identity = 182/310 (58.71%), Postives = 230/310 (74.19%), Query Frame = 0

Query: 1   MGKRSP-PGDIESMSYDVRKPLSMPPP--PSYYPPPPKPWFPWLVPLTFLANIAMFVFTL 60
           MGKR P P DIE+           PPP  P + PP P PW  WLVPL   AN   F  T+
Sbjct: 1   MGKRPPIPPDIEN---------GPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTM 60

Query: 61  YENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWR 120
           Y N+CPA    + CL +  LGR SFQP +EN LLGPSI TL++ GAL++  +VE GE WR
Sbjct: 61  YVNDCPAR--SDECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGERWR 120

Query: 121 LVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSE 180
           L+SC+WLH G +HL+ANM+SL+ IG+RLEQEFGF++IG LYV+SG GGSL+S L+ +  E
Sbjct: 121 LISCIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDSQGE 180

Query: 181 NPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSA 240
              +SVGASGALFGLLGAMLSELITNWTIY NKC A ++LILII LNL+VGF+P +DNSA
Sbjct: 181 R--VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSA 240

Query: 241 HIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVK-RKSKHKCYQYFLLIVALILLIFG 300
           H GGF++GF LGF+LL++PQ+GYVN KYIP G D+K +KSKHKCYQ+     +L +L+ G
Sbjct: 241 HFGGFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAG 296

Query: 301 YVSGLTRLYK 307
           +++G T+L +
Sbjct: 301 FIAGYTKLLR 296

BLAST of CmaCh08G006770 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 329.7 bits (844), Expect = 2.6e-90
Identity = 173/275 (62.91%), Postives = 206/275 (74.91%), Query Frame = 0

Query: 33  PPKPWFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLG 92
           P K WFPWLVP   +ANIA+F  +++ NNCP         F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 93  PSISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCA 212
           +IG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK A
Sbjct: 170 RIGLLYMISGFGGSLLSSLFNRAG----ISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 AFISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAG-AD 272
           A ++LI IIA+NLAVG +PH+DN AH+GGF SGFLLGF+ LI+PQ+GY N +  P G A 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
              KSKHK YQY L I +L+LLI GY +GL  L +
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLR 317

BLAST of CmaCh08G006770 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 311.6 bits (797), Expect = 7.2e-85
Identity = 171/296 (57.77%), Postives = 202/296 (68.24%), Query Frame = 0

Query: 33  PPKPWFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFYPELGRFSFQPFQENPLLG 92
           P K WFPWLVP   +ANIA+F  +++ NNCP         F   LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSAYCLARF---LGRFAFQPMKENPLLG 109

Query: 93  PSISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKC- 212
           +IG LY++SGFGGSLLSSL         ISVGASGALFGLLGAMLSEL+TNWTIY+NK  
Sbjct: 170 RIGLLYMISGFGGSLLSSLFNRAG----ISVGASGALFGLLGAMLSELLTNWTIYANKVA 229

Query: 213 ---------------------------AAFISLILIIALNLAVGFIPHIDNSAHIGGFVS 272
                                      AA ++LI IIA+NLAVG +PH+DN AH+GGF S
Sbjct: 230 KSSLVKQAALSMNDVSIMSLVFLHLQFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTS 289

Query: 273 GFLLGFILLIQPQFGYVNHKYIPAG-ADVKRKSKHKCYQYFLLIVALILLIFGYVS 300
           GFLLGF+ LI+PQ+GY N +  P G A    KSKHK YQY L I +L+LLI GY S
Sbjct: 290 GFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGYTS 338

BLAST of CmaCh08G006770 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 308.5 bits (789), Expect = 6.1e-84
Identity = 156/273 (57.14%), Postives = 206/273 (75.46%), Query Frame = 0

Query: 37  WFPWLVPLTFLANIAMFVFTLYENNCPAAIGGNRCLFY---PELGRFSFQPFQENPLLGP 96
           W  WLVP+  +AN+A+FV  ++ NNCP     +R   +     LGR SF+P + NPL GP
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFGP 112

Query: 97  SISTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLK 156
           S  TL++ GAL+   VVE  EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF++
Sbjct: 113 SSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVR 172

Query: 157 IGCLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAA 216
           IG +Y+LSG GGS+LSSL I  S    ISVGASGALFGLLG+MLSEL TNWTIYSNK AA
Sbjct: 173 IGVIYLLSGIGGSVLSSLFIRNS----ISVGASGALFGLLGSMLSELFTNWTIYSNKIAA 232

Query: 217 FISLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVK 276
            ++L+ +I +NLA+G +PH+DN AH+GGFV+GFLLGFILL +PQF ++  +++P G  + 
Sbjct: 233 LLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL- 292

Query: 277 RKSKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
            + K+K YQY L +++L+LLI G+V  L  L++
Sbjct: 293 -RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

BLAST of CmaCh08G006770 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 307.0 bits (785), Expect = 1.8e-83
Identity = 152/271 (56.09%), Postives = 203/271 (74.91%), Query Frame = 0

Query: 37  WFPWLVPLTFLANIAMFVFTLYENNCPAAI-GGNRCLFYPELGRFSFQPFQENPLLGPSI 96
           W  WL+P   +AN+A+F+  ++ N+CP  I G N+      LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  STLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIG 156
           STL++ GAL+   VV   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+++G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSSLSINPSENPIISVGASGALFGLLGAMLSELITNWTIYSNKCAAFI 216
            +Y++SG GGS+LSSL +  S    ISVGASGALFGLLGAMLSEL+TNWTIY+NK AA I
Sbjct: 151 LIYLISGLGGSILSSLFLQES----ISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFIPHIDNSAHIGGFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRK 276
           +L+ IIA+NLA+G +P +DN AHIGGF++GF LGF+LL++PQ+G+   +   +    + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASRTNTS----RTK 270

Query: 277 SKHKCYQYFLLIVALILLIFGYVSGLTRLYK 307
            K+  YQY L +V+++LL+ G    L  L+K
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SSR01.8e-9658.71RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1[more]
Q0WQX73.6e-8962.91RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
Q9LYP18.6e-8357.14RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
Q9CAN12.5e-8256.09RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
F4JBM44.0e-8054.61RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KMF52.7e-180100.00RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1[more]
A0A6J1HKQ14.3e-17898.74RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1[more]
A0A5A7UZ092.5e-15784.95RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold33... [more]
A0A6J1JR476.0e-15686.12RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488148 PE=3 SV=1[more]
A0A6J1GPL61.8e-15586.12RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111456374 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023000373.15.6e-180100.00RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima][more]
XP_022964405.18.9e-17898.74RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein... [more]
XP_023514894.11.2e-17798.42RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo][more]
XP_038899775.11.7e-15785.80RHOMBOID-like protein 5 [Benincasa hispida][more]
XP_038888787.13.0e-15785.49RHOMBOID-like protein 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G52580.11.3e-9758.71RHOMBOID-like protein 5 [more]
AT2G29050.12.6e-9062.91RHOMBOID-like 1 [more]
AT2G29050.27.2e-8557.77RHOMBOID-like 1 [more]
AT5G07250.16.1e-8457.14RHOMBOID-like protein 3 [more]
AT1G63120.11.8e-8356.09RHOMBOID-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 38..255
e-value: 1.1E-38
score: 134.9
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 35..257
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 111..255
e-value: 4.4E-41
score: 140.4
NoneNo IPR availablePANTHERPTHR22936:SF87RHOMBOID-LIKE PROTEIN 5coord: 1..306
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 1..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G006770.1CmaCh08G006770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity